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https://www.readbyqxmd.com/read/28743079/impact-of-lyophilized-lactobacillus-salivarius-dspv-001p-administration-on-growth-performance-microbial-translocation-and-gastrointestinal-microbiota-of-broilers-reared-under-low-ambient-temperature
#1
J E Blajman, C A Olivero, M L Fusari, J A Zimmermann, E Rossler, A P Berisvil, A Romero Scharpen, D M Astesana, L P Soto, M L Signorini, M V Zbrun, L S Frizzo
This study was undertaken with the aim of investigating the effects of dietary supplementation of probiotic strain Lactobacillus salivarius DSPV 001P on growth performance, microbial translocation, and gastrointestinal microbiota of broilers reared under low ambient temperature. Two hundred and forty, one-day-old male Cobb broilers were randomly distributed into two treatment groups, a probiotic group and a control group, with four replicates per treatment and 30 broilers per replicate. The temperature of the broiler house was maintained at 18-22°C during the first three weeks, after which the temperature was at range of 8°C to 12°C...
July 19, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28742822/metal-ion-coordination-in-the-e-coli-nudix-hydrolase-dihydroneopterin-triphosphate-pyrophosphatase-new-clues-into-catalytic-mechanism
#2
Shannon E Hill, Elaine Nguyen, Chiamaka U Ukachukwu, Dana M Freeman, Stephen Quirk, Raquel L Lieberman
Dihydroneopterin triphosphate pyrophosphatase (DHNTPase), a member of the Mg2+ dependent Nudix hydrolase superfamily, is the recently-discovered enzyme that functions in the second step of the pterin branch of the folate biosynthetic pathway in E. coli. DHNTPase is of interest because inhibition of enzymes in bacterial folate biosynthetic pathways is a strategy for antibiotic development. We determined crystal structures of DHNTPase with and without activating, Mg2+-mimicking metals Co2+ and Ni2+. Four metal ions, identified by anomalous scattering, and stoichiometrically confirmed in solution by isothermal titration calorimetry, are held in place by Glu56 and Glu60 within the Nudix sequence motif, Glu117, waters, and a sulfate ion, of which the latter is further stabilized by a salt bridge with Lys7...
2017: PloS One
https://www.readbyqxmd.com/read/28742106/epidemiology-of-enteroaggregative-escherichia-coli-infections-and-associated-outcomes-in-the-mal-ed-birth-cohort
#3
Elizabeth T Rogawski, Richard L Guerrant, Alexandre Havt, Ila F N Lima, Pedro H Q S Medeiros, Jessica C Seidman, Benjamin J J McCormick, Sudhir Babji, Dinesh Hariraju, Ladaporn Bodhidatta, Jasmin Shrestha, Japhat Anania, Athanasia Maro, Amidou Samie, Pablo Peñataro Yori, Shahida Qureshi, Mustafa Mahfuz, Pascal O Bessong, Margaret N Kosek, Tahmeed Ahmed, Zulfiqar A Bhutta, Dennis R Lang, Michael Gottlieb, Eric R Houpt, Aldo A M Lima
BACKGROUND: Enteroaggregative E. coli (EAEC) have been associated with mildly inflammatory diarrhea in outbreaks and in travelers and have been increasingly recognized as enteric pathogens in young children with and without overt diarrhea. We examined the risk factors for EAEC infections and their associations with environmental enteropathy biomarkers and growth outcomes over the first two years of life in eight low-resource settings of the MAL-ED study. METHODS: EAEC infections were detected by PCR gene probes for aatA and aaiC virulence traits in 27,094 non-diarrheal surveillance stools and 7,692 diarrheal stools from 2,092 children in the MAL-ED birth cohort...
July 24, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28742064/the-effect-of-biosilver-nanoparticles-on-different-bacterial-strains-metabolism-reflected-in-their-vocs-profiles
#4
Boguslaw Buszewski, Ileana Andreea Ratiu, Maciej Milanowski, Pawel Pomastowski, Tomasz Ligor
Silver nanoparticles use has spawned during the last years a growing interest for many researchers, due to their bacteriostatic and bactericidal properties and synergetic effects, when they are used together with antibiotics, for an increased efficiency and less adverse reactions in treatment of bacterial infections. GC-MS (gas chromatography coupled with mass spectrometry), which is considered "the golden standard" in chemical analysis has proven to be a reliable instrument, perfectly suitable for clinical analysis...
July 25, 2017: Journal of Breath Research
https://www.readbyqxmd.com/read/28742000/high-prevalence-of-multidrug-resistant-escherichia-coli-isolates-from-children-with-and-without-diarrhoea-and-their-susceptibility-to-the-antibacterial-activity-of-extracts-fractions-of-fruits-native-to-mexico
#5
Magdalena de Jesús Uribe-Beltrán, Yesmi Patricia Ahumada-Santos, Sylvia Páz Díaz-Camacho, Carlos Alberto Eslava-Campos, Jesús Ernesto Reyes-Valenzuela, María Elena Báez-Flores, Ignacio Osuna-Ramírez, Francisco Delgado-Vargas
PURPOSE: This paper aims to evaluate the antimicrobial resistance of Esherichia coli isolates from children under 5 years old, with and without diarrhoea, who were hospital outpatients in Culiacan, Sinaloa, Mexico. It also looks at the antimicrobial activity of fruit extracts against selected multidrug-resistant (MDR) E. coli strains. METHODOLOGY: A total of 205 E. coli isolates from stool samples were collected from 94 children under 5 years old who were outpatients from two hospitals in the city of Culiacan, Sinaloa, Mexico, during the autumn/winter of 2003/04; their resistance profiles to 19 commercial antimicrobials were investigated using the Kirby-Bauer method...
July 26, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28741927/rapid-antibiotic-susceptibility-testing-of-uropathogenic-e-coli-by-tracking-sub-micron-scale-motion-of-single-bacterial-cells
#6
Karan Syal, Simon Shen, Yunze Yang, Shaopeng Wang, Shelley E Haydel, Nongjian Tao
To combat antibiotic resistance, a rapid antibiotic susceptibility testing (AST) technology that can identify resistant infections at disease onset is required. Current clinical AST technologies take 1-3 days, which is often too slow for accurate treatment. Here we demonstrate a rapid AST method by tracking sub-µm scale bacterial motion with an optical imaging and tracking technique. We apply the method to clinically relevant bacterial pathogens, Escherichia coli O157: H7 and uropathogenic E. coli (UPEC) loosely tethered to a glass surface...
July 25, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28741705/house-dust-mites-as-potential-carriers-for-ige-sensitisation-to-bacterial-antigens
#7
Sheron Dzoro, Irene Mittermann, Yvonne Resch, Susanne Vrtala, Marion Nehr, Alexander M Hirschl, Gustav Wikberg, Lena Lundeberg, Catharina Johansson, Annika Scheynius, Rudolf Valenta
BACKGROUND: IgE-reactivity to antigens from gram-positive and negative bacteria is common in patients suffering from respiratory and skin manifestations of allergy, but the routes and mechanisms of sensitisation are not fully understood. The analysis of the genome, transcriptome and microbiome of house dust mites (HDM) has shown that S. aureus and E. coli species are abundant bacteria within the HDM microbiome. Therefore, our aim was to investigate if HDM are carriers of bacterial antigens leading to IgE sensitisation in patients suffering from atopic dermatitis...
July 25, 2017: Allergy
https://www.readbyqxmd.com/read/28741465/engineering-of-chimeric-protein-based-on-e-protein-domain-iii-of-tick-borne-encephalitis-virus-and-ompf-porin-of-yersinia-pseudotuberculosis
#8
Anna M Stenkova, Natalia S Chopenko, Ludmila A Davydova, Andrey N Mazeika, Evgeniya P Bystritskaya, Olga Y Portnyagina, Stanislav D Anastyuk, Dmitrii S Kulbatskii, Ekaterina N Lyukmanova, Dmitriy A Dolgikh, Eduard Y Kostetsky, Nina M Sanina
Tick-borne encephalitis poses a serious public health threat in the endemic regions. The disease treatment is restricted to symptomatic therapy, so great expectations are in the development of the prophylactic and therapeutic vaccines. The domain III of E protein of the tick-borne encephalitis virus is the main antigenic domain which includes virus-specific epitopes recognized by neutralizing antibodies. We have expressed, isolated and characterized the chimeric protein based on the fusion of domain III of E protein of the tick-borne encephalitis virus and bacterial porin OmpF from Yersinia pseudotuberculosis...
July 24, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28741464/expression-of-mastoparan-b-a-venom-peptide-via-escherichia-coli-c43-de3-coupled-with-an-artificial-oil-body-cyanogen-bromide-technology-platform
#9
Tzyy-Rong Jinn, Sheng-Kuo Hsieh, Yu-Jen Yu, Nou-Ying Tang, Jhao-Ren Lin
Here, we successfully use the oleosin-based fusion expression strategy coupled with the artificial oil body (AOB)-cyanogen bromide (CNBr) platform to produce bioactive MPB peptide which, in a manner identical to that of its native counterpart. In this study, the MPB peptide was interlinked with recombinant oleosin (rOle(127M→L)) by a methionine residue, a CNBr cleavage site. The recombinant rOle(127M→L)-MPB fusion gene was cloned into the pET30a(+) vector and was expressed in E. coli (DE3) after IPTG induction under optimized conditions...
July 24, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28741460/discovery-and-biochemical-characterization-of-udp-glucose-dehydrogenase-from-akkermansia-muciniphila
#10
Shuang Wei, Xiao-Yang Zhang, Yue Sun, Louis Patrick Conway, Li Liu
We report the discovery and characterization of a UDP-glucose dehydrogenase (AmUGD) from Akkermansia muciniphila, a bacterium originally isolated from human faeces in an anaerobic medium containing gastric mucin as the sole carbon source. The recombinant form of the protein was expressed in E. coli and the purified enzyme exhibited optimum levels of activity at 37°C and pH 9.0. While the enzyme is active in the absence of metal ions, the presence of Zn2+ ions results in markedly enhanced levels of catalysis...
July 24, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28741348/super-resolution-far-field-infrared-imaging-by-photothermal-heterodyne-imaging
#11
Zhongming Li, Kyle Aleshire, Masaru Kuno, Gregory V Hartland
Infrared (IR) imaging provides chemical-specific information without the need for exogenous labels. Conventional far-field IR imaging techniques are diffraction limited, which means an effective spatial resolution of > 5 μm with currently available optics. In this paper we present a novel far-field IR imaging technique based on photothermal heterodyne imaging (IR-PHI). In our version of IR-PHI an IR pump laser excites the sample, causing a small temperature rise that is detected by a counter-propagating visible probe beam...
July 25, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28741290/biochemical-characterization-of-lysine-acetylation-of-tyrosyl-trna-synthetase-in-escherichia-coli
#12
Sumana Venkat, Caroline Gregory, Qinglei Gan, Chenguang Fan
Aminoacyl-tRNA synthetases (AARSs) play essential roles in protein synthesis. As a member of the AARS family, the tyrosyl-tRNA synthetase (TyrRS) in Escherichia coli has been shown to be acetylated at multiple lysine residues by proteomic studies. However, these putative acetylation targets have not been biochemically characterized yet. In this study, we applied the genetic code expansion strategy to site-specifically incorporate Nε-acetyl-L-lysine into selected positions of TyrRS for in vitro characterization...
July 24, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28740758/identification-and-characterization-of-streptomyces-flavogriseus-nj-4-as-a-novel-producer-of-actinomycin-d-and-holomycin
#13
Zhaohui Wei, Chao Xu, Juan Wang, Fengxia Lu, Xiaomei Bie, Zhaoxin Lu
This paper is the first public report that Streptomyces flavogriseus can produce both actinomycin D and holomycin. The actinomycete strain NJ-4 isolated from the soil of Nanjing Agricultural University was identified as S. flavogriseus. This S. flavogriseus strain was found for the first time to produce two antimicrobial compounds that were identified as actinomycin D and holomycin. GS medium, CS medium and GSS medium were used for the production experiments. All three media supported the production of actinomycin D, while holomycin was detected only in GS medium and was undetectable by HPLC in the CS and GSS media...
2017: PeerJ
https://www.readbyqxmd.com/read/28740729/role-of-the-inserted-%C3%AE-helical-domain-in-e-coli-atp-dependent-lon-protease-function
#14
A M Kudzhaev, A G Andrianova, E S Dubovtseva, O V Serova, T V Rotanova
Multidomain ATP-dependent Lon protease of E. coli (Ec-Lon) is one of the key enzymes of the quality control system of the cellular proteome. A recombinant form of Ec-Lon with deletion of the inserted characteristic α-helical HI(CC) domain (Lon-dHI(CC)) has been prepared and investigated to understand the role of this domain. A comparative study of the ATPase, proteolytic, and peptidase activities of the intact Lon protease and Lon-dHI(CC) has been carried out. The ability of the enzymes to undergo autolysis and their ability to bind DNA have been studied as well...
April 2017: Acta Naturae
https://www.readbyqxmd.com/read/28739824/epigenetic-mechanisms-regulate-innate-immunity-against-uropathogenic-and-commensal-like-escherichia-coli-in-the-surrogate-insect-model-galleria-mellonella
#15
Miriam Heitmueller, André Billion, Ulrich Dobrindt, Andreas Vilcinskas, Krishnendu Mukherjee
Innate immunity-related genes in humans are activated during urinary tract infections (UTIs) caused by pathogenic strains of Escherichia coli but are suppressed by commensals. Epigenetic mechanisms play a pivotal role in the regulation of gene expression in response to environmental stimuli. To determine whether epigenetic mechanisms can explain the different behaviors of pathogenic and commensal bacteria, we infected larvae of the greater wax moth Galleria mellonella, a widely-used model insect host, with a uropathogenic E...
July 24, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28739804/role-of-the-disulfide-bond-in-stabilizing-and-folding-of-the-fimbrial-protein-drae-from-uropathogenic-escherichia-coli
#16
Justyna Pilipczuk, Beata Zalewska-Piątek, Piotr Bruździak, Jacek Czub, Milosz Wieczór, Marcin Olszewski, Marta Wanarska, Bogdan Nowicki, Danuta Augustin-Nowacka, Rafal Piątek
Dr fimbriae are homopolymeric adhesive organelles of uropathogenic Escherichia coli composed of DraE subunits, responsible for the attachment to host cells. These structures are characterized by enormously high stability resulting from the structural properties of an Ig-like fold of DraE. One feature of DraE and other fimbrial subunits that makes them peculiar among Ig-like domain-containing proteins is a conserved disulfide bond that joins their A and B strands. Here, we investigated how this disulfide bond affects the stability and folding/unfolding pathway of DraE...
July 24, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28739800/cell-free-production-of-a-functional-oligomeric-form-of-a-chlamydia-major-outer-membrane-protein-momp-for-vaccine-development
#17
Wei He, Martina Felderman, Angela C Evans, Jia Geng, David Homan, Feliza Bourguet, Nicholas O Fischer, Yuanpei Li, Kit S Lam, Aleksandr Noy, Li Xing, R Holland Cheng, Amy Rasley, Craig D Blanchette, Kurt Kamrud, Nathanial Wang, Heather Gouvis, Todd C Peterson, Bolyn Hubby, Matthew A Coleman
Chlamydia is a prevalent sexually transmitted disease that infects more than 100 million people worldwide. Although most individuals infected with Chlamydia trachomatis are initially asymptomatic, symptoms can arise if left undiagnosed. Long-term infection can result in debilitating conditions such as pelvic inflammatory disease, infertility, and blindness. Chlamydia infection, therefore, constitutes a significant public health threat, underscoring the need for a Chlamydia-specific vaccine. Chlamydia strains express a major outer-membrane protein (MOMP) that has been shown to be an effective vaccine antigen...
July 24, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28739488/nanosilver-pmma-composite-coating-optimized-to-provide-robust-antibacterial-efficacy-while-minimizing-human-bone-marrow-stromal-cell-toxicity
#18
Peter E Petrochenko, Jiwen Zheng, Brendan J Casey, Reza Bayati, Roger J Narayan, Peter L Goering
Porous PMMA is a versatile biomaterial with good biocompatibility but high susceptibility to bacterial colonization, which we mitigated by utilizing immobilized antimicrobial silver nanoparticles (AgNPs). A uniform porous thin film was deposited onto silicon wafers by simultaneously ablating PMMA and silver (Ag) using pulsed laser deposition (PLD) optimized for minimal human cell toxicity and antibacterial efficacy. PMMA without Ag became heavily colonized by E. coli in simulated dynamic conditions, while Ag-containing samples prevented all colonization...
July 21, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28739428/novel-class-1-integrons-and-sequence-types-in-vim-2-and-vim-11-producing-clinical-strains-of-enterobacter-cloacae
#19
D De Belder, D Faccone, N Tijet, R Melano, M Rapoport, A Petroni, C Lucero, F Pasteran, A Corso, S A Gomez
All VIM-producing Enterobacteriaceae (six Enterobacter cloacae) submitted to the Argentinian Reference Laboratory in Antimicrobial Resistance in the period 2008-13 were characterized. The isolates were referred from 6 nosocomial institutions located in 5 different cities across the country. All isolates showed carbapenem disk diffusion inhibition zones ≤22mm and synergism between a carbapenem disk and EDTA/SMA. The six isolates were PCR positive for blaVIM. Imipenem MICs were ≤1 to 8μg/ml. Typing by PFGE and MLST distinguished six pulsotypes and sequence types with blaVIM located on novel class 1 integron arrays: ECL-1: ST182, In883; ECL-2, ST90, In885; ECL-3, ST88, In346 with blaVIM-11; ECL-4, ST184, In900; ECL-5, ST749-new, In900; ECL-6, ST91 and uncharacterized In...
July 21, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28739224/esbl-producing-escherichia-coli-and-klebsiella-pneumoniae-the-most-prevalent-clinical-isolates-obtained-between-2005-and-2012-in-mexico
#20
Humberto Barrios, Ulises Garza-Ramos, Ilse Mejia-Miranda, Fernando Reyna-Flores, Alejandro Sánchez-Perez, Dalila Mosqueda-García, Jesus Silva-Sanchez
OBJECTIVES: To identify the prevalence of ESBL genes in the principal group of Enterobacteriaceae causing nosocomial infections and to identify the phylogenetic group in E. coli isolates. METHODS: There were collected 1084 ESBL-producing Enterobacteriaceae isolates during 2005 to 2012 from adult patients from 14 hospitals and corresponding to eight states and five regions (SE, S, N, W and NW) in Mexico. The CTX-M-(CTX-M-1 group), SHV-, TLA- and GES-type ESBLs genes were screened...
July 21, 2017: Journal of Global Antimicrobial Resistance
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