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https://www.readbyqxmd.com/read/28214753/structural-and-functional-insights-into-corrinoid-iron-sulfur-protein-from-human-pathogen-clostridium-difficile
#1
Yaozhu Wei, Xiaofei Zhu, Sixue Zhang, Xiangshi Tan
The human pathogen Clostridium difficile infection (CDI) is one of the most important healthcare-associated infections. The Wood-Ljungdahl pathway, which is responsible for Acetyl-CoA biosynthesis, is essential for the survival of the pathogen and is absent in humans. The key proteins and enzymes involved in the pathway are attractive targets for the treatment of CDI. Corrinoid iron-sulfur protein (CoFeSP) is a key protein and acts as a methyl transformer in the Wood-Ljungdahl pathway. In this study, CoFeSP from Clostridium difficile (CoFeSPCd) was cloned, expressed in E...
February 13, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28214718/alkyl-substituent-effect-on-photosensitized-inactivation-of-escherichia-coli-by-pyridinium-bonded-p-porphyrins
#2
Jin Matsumoto, Yusaku Suemoto, Hiroki Kanemaru, Kyosuke Takemori, Masato Shigehara, Akari Miyamoto, Haruhiko Yokoi, Masahide Yasuda
Activity of singlet oxygen sensitizers for photoinactivation of bacteria and photodynamic therapy of tumor cells has been evaluated using nonpathogenic model cells, such as Escherichia coli, Saccharomyces cerevisiae, and HeLa cells. Among them, E. coli, gram-negative bacterium, has complex membrane structures in the cell wall, resulting in an impermeable barrier to antimicrobial agents. Therefore, few singlet oxygen sensitizers have photoinactivation activities toward E. coli at low concentrations. Recently polycationic porphyrins have received much attention as a new type of singlet oxygen sensitizers because they have strong binding affinities for DNA and proteins...
February 6, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28214713/comparative-analysis-of-metagenomes-of-italian-top-soil-improvers
#3
Federica Gigliucci, Gianfranco Brambilla, Rosangela Tozzoli, Valeria Michelacci, Stefano Morabito
Biosolids originating from Municipal Waste Water Treatment Plants are proposed as top soil improvers (TSI) for their beneficial input of organic carbon on agriculture lands. Their use to amend soil is controversial, as it may lead to the presence of emerging hazards of anthropogenic or animal origin in the environment devoted to food production. In this study, we used a shotgun metagenomics sequencing as a tool to perform a characterization of the hazards related with the TSIs. The samples showed the presence of many virulence genes associated to different diarrheagenic E...
February 16, 2017: Environmental Research
https://www.readbyqxmd.com/read/28214695/the-effects-of-atmospheric-pressure-cold-plasma-treatment-on-microbiological-physical-chemical-and-sensory-characteristics-of-vacuum-packaged-beef-loin
#4
A Bauer, Y Ni, S Bauer, P Paulsen, M Modic, J L Walsh, F J M Smulders
Effects on vacuum packaged and non-packaged beef longissimus samples exposed to atmospheric cold plasma (ACP) generated at different powers were studied over a 10day period of vacuum-, and a subsequent 3day period of aerobic storage. Exposure of non-covered beef samples under high power ACP conditions resulted in increased a*, b*, Chroma and Hue values, but ACP treatment of packaged loins did not impact colour (L*, a*, b*, Chroma, Hue), lipid peroxidation, sarcoplasmic protein denaturation, nitrate/nitrite uptake, or myoglobin isoform distribution...
February 8, 2017: Meat Science
https://www.readbyqxmd.com/read/28214589/enhanced-expression-and-purification-of-camelid-single-domain-vhh-antibodies-from-classical-inclusion-bodies
#5
Maristella Maggi, Claudia Scotti
Single domain antibodies (sdAbs) are small antigen-binding domains derived from naturally occurring, heavy chain-only immunoglobulins isolated from camelid and sharks. They maintain the same binding capability of full-length IgGs but with improved thermal stability and permeability, which justifies their scientific, medical and industrial interest. Several described recombinant forms of sdAbs have been produced in different hosts and with different strategies. Here we present an optimized method for a time-saving, high yield production and extraction of a poly-histidine-tagged sdAb from Escherichia coli classical inclusion bodies...
February 15, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28214542/several-hpept1-transported-drugs-are-substrates-of-the-e-coli-proton-coupled-oligopeptide-transporter-ydgr
#6
Bala K Prabhala, Nanda G Aduri, Mazhar Iqbal, Moazur Rahman, Michael Gajhede, Paul R Hansen, Osman Mirza
Proton-dependent oligopeptide transporters (POTs) are secondary active transporters found in all kingdoms of life. POTs utilize the proton electrochemical gradient for the uptake of nutrient dipeptides and tripeptides. The human POT hPepT1 is known to transport a number of drugs. As part of ongoing studies on substrate specificities of POTs from E. coli, our aim in this study was to investigate whether bacterial POTs could also transport these drugs. For this, we selected the common orally administered drugs sulpiride, bestatin, valacyclovir, ampicillin and oseltamivir, that are all transported by hPepT1...
February 15, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28214340/molecular-tiling-on-the-surface-of-a-bacterial-spore-the-exosporium-of-the-bacillus-anthracis-cereus-thuringiensis-group
#7
Cassandra Terry, Shuo Jiang, David S Radford, Wan Qiang, Svetomir Tzokov, Anne Moir, Per A Bullough
Bacteria of the genera Bacillus and Clostridium form highly resistant spores, which in the case of some pathogens act as the infectious agents. An exosporium forms the outermost layer of some spores; it plays roles in protection, adhesion, dissemination, host targeting in pathogens, and germination control. The exosporium of the Bacillus cereus group, including the anthrax pathogen, contains a 2D-crystalline basal layer, overlaid by a hairy nap. BclA and related proteins form the hairy nap, and require ExsFA (BxpB) for their localisation on the basal layer...
February 18, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28214242/injury-and-recovery-of-escherichia-coli-atcc25922-cells-treated-by-high-hydrostatic-pressure-at-400-600%C3%A2-mpa
#8
Keitarou Kimura, Kazuya Morimatsu, Takashi Inaoka, Kazutaka Yamamoto
Escherichia coli cells were inactivated by high hydrostatic pressure (HHP) at 400-600 MPa and their recovery under various conditions was evaluated by colony counting and flow cytometer (FCM) analyses. The lag time in colony formation and an improved recovery of cells under less oxidative conditions (pyruvate addition to the medium and incubation in anaerobic conditions) were observed for HHP treated cells, which indicated that a significant portion of cells were injured and recovered during incubation after HHP treatment...
February 14, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28214232/new-nitrofurans-amenable-by-isocyanide-multicomponent-chemistry-are-active-against-multidrug-resistant-and-poly-resistant-mycobacterium-tuberculosis
#9
Mikhail Krasavin, Vladislav Parchinsky, Grigory Kantin, Olga Manicheva, Marine Dogonadze, Tatiana Vinogradova, Bianka Karge, Mark Brönstrup
A set of structurally diverse N-amino δ-lactams decorated with a 5-nitro-2-furyl moiety was synthesized using isocyanide-based multicomponent chemistry and evaluated for antibacterial activity. Three compounds displayed a selective and potent (MIC 22-33μM) inhibition of M. tuberculosis H37Rv strain growth, while other Gram-positive (MRSA and E. faecium) or Gram-negative (E. coli, P. aeruginosa, A. baumannii, K. pneumoniae) pathogens were not affected. The compounds also displayed moderate-low cytotoxicity, as demonstrated in cell line viability assays...
February 4, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28214224/escherichia-coli-sequence-type-73-as-a-cause-of-community-acquired-urinary-tract-infection-in-men-and-women-in-rio-de-janeiro-brazil
#10
Ana Paula de Souza da-Silva, Viviane Santos de Sousa, Natacha Martins, Rubens Clayton da Silva Dias, Raquel Regina Bonelli, Lee W Riley, Beatriz Meurer Moreira
Escherichia coli clones ST131, ST69, ST95, and ST73 are frequent causes of urinary tract infections (UTI) and bloodstream infections. Specific clones and virulence profiles of E. coli causing UTI in men has been rarely described. The aim of this study was to characterize patient and clonal characteristics of community-acquired UTI caused by E. coli in men (n=12) and women (n=127) in Rio de Janeiro, Brazil, complementing a previous work. We characterized isolates in phylogenetic groups, ERIC2-PCR and PFGE types, MLST, genome similarity and virulence gene-profiles...
February 7, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28213899/human-influence-and-biotic-homogenization-drive-the-distribution-of-escherichia-coli-virulence-genes-in-natural-habitats
#11
Adriana Cabal, Joaquin Vicente, Julio Alvarez, Jose Angel Barasona, Mariana Boadella, Lucas Dominguez, Christian Gortazar
Cattle are the main reservoirs for Shiga-toxin-producing Escherichia coli (STEC), the only known zoonotic intestinal E. coli pathotype. However, there are other intestinal pathotypes that can cause disease in humans, whose presence has been seldom investigated. Thus, our aim was to identify the effects of anthropic pressure and of wild and domestic ungulate abundance on the distribution and diversity of the main human E. coli pathotypes and nine of their representative virulence genes (VGs). We used a quantitative real-time PCR (qPCR) for the direct detection and quantification of the genus-specific gene uidA, nine E...
February 18, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28213539/critical-concentration-of-lecithin-enhances-the-antimicrobial-activity-of-eugenol-against-escherichia-coli
#12
Haoshu Zhang, Edward G Dudley, P Michael Davidson, Federico Harte
Lecithin is a natural emulsifier used in a wide range of food and non-food applications to improve physical stability with no known bioactive effects. In this study, the effect of lecithin on the antimicrobial performance of a constant eugenol concentration was tested against three Escherichia coli strains (C600, 0.1229, and O157:H7 strain ATCC 700728). This was the first study focusing on lecithin at concentrations below those commonly used in foods to improve the stability of oil in water emulsions (≤ 10mg/100ml)...
February 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28213537/ketoacylsynthase-domains-of-a-pufa-synthase-in-thraustochytrium-can-effectively-function-as-stand-alone-enzymes-in-escherichia-coli
#13
Xi Xie, Dauenpen Meesapyodsuk, Xiao Qiu
Thraustochytrium sp. 26185 accumulates a high level of docosahexaenoic acid (DHA), a nutritionally important ω-3 very long chain unsaturated fatty acid (VLCPUFA) synthesized primarily by polyunsaturated fatty acid (PUFA) synthase, a Type I polyketide synthase-like mega-enzyme. The PUFA synthase in this species comprises three large subunits each with multiple catalytic domains. It was hypothesized that among these domains, ketoacylsynthase (KS) domains might be critical for catalyzing the condensation of specific unsaturated acyl-ACPs with malonyl-ACP, whereby retaining double bonds in an extended acyl chain...
February 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28213234/dna-wrapped-multi-walled-carbon-nanotube-modified-electrochemical-biosensor-for-the-detection-of-escherichia-coli-from-real-samples
#14
Dilsat Ozkan-Ariksoysal, Yasin Ugur Kayran, Fethiye Ferda Yilmaz, Anton Alexandru Ciucu, Iulia Gabriela David, Vasile David, Mine Hosgor-Limoncu, Mehmet Ozsoz
This paper introduces DNA-wrapped multi-walled carbon nanotube (MWCNT)-modified genosensor for the detection of Escherichia coli (E. coli) from polymerase chain reaction (PCR)-amplified real samples while Staphylococcus aureus (S. aureus) was used to investigate the selectivity of the biosensor. The capture probe specifically recognizing E. coli DNA and it was firstly interacted with MWCNTs for wrapping of single-stranded DNA (ssDNA) onto the nanomaterial. DNA-wrapped MWCNTs were then immobilised on the surface of disposable pencil graphite electrode (PGE) for the detection of DNA hybridization...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28213165/hbvcircle-a-novel-tool-to-investigate-hepatitis-b-virus-covalently-closed-circular-dna
#15
Zhipeng Yan, Jing Zeng, Youjun Yu, Kunlun Xiang, Hui Hu, Xue Zhou, Lili Gu, Li Wang, Jie Zhao, John A T Young, Lu Gao
BACKGROUND & AIMS: Hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) persists as a stable episome in infected hepatocytes and serves as a template for the transcription of all viral genes. Due to the narrow host range of HBV, the development of a robust mouse model that supports cccDNA-dependent viral replication is a key hurdle in the development of novel HBV therapeutics. This study aimed to develop a novel tool to investigate HBV cccDNA. METHODS: Through minicircle technology, HBVcircle, a recombinant cccDNA, was easily generated and extracted from a genetically engineered E...
February 14, 2017: Journal of Hepatology
https://www.readbyqxmd.com/read/28213103/expression-and-characterization-of-haemathrins-madanin-like-thrombin-inhibitors-isolated-from-the-salivary-gland-of-tick-haemaphysalis-bispinosa-acari-ixodidae
#16
Rajeev Kungur Brahma, Guillaume Blanchet, Simran Kaur, R Manjunatha Kini, Robin Doley
Saliva of hematophagous animals, such as ticks, is an excellent source of anticoagulant proteins and polypeptides. Here we describe the identification and characterization of two thrombin inhibitors named as haemathrin 1 and 2 from the salivary gland of tick Haemaphysalis bispinosa using genomic approach. Haemathrins are cysteine-less peptide anticoagulants, which share about 65-70% identity with madanins, and belong to inhibitor I53 superfamily of inhibitors of the MEROPS database. Haemathrins were overexpressed in E...
January 31, 2017: Thrombosis Research
https://www.readbyqxmd.com/read/28213040/the-locus-of-heat-resistance-lhr-mediates-heat-resistance-in-salmonella-enterica-escherichia-coli-and-enterobacter-cloacae
#17
Ryan G Mercer, Brian D Walker, Xianqin Yang, Lynn M McMullen, Michael G Gänzle
Enterobacteriaceae comprise food spoilage organisms as well as food-borne pathogens including Escherichia coli. Heat resistance in E. coli was attributed to a genomic island called the locus of heat resistance (LHR). This genomic island is also present in several other genera of Enterobacteriaceae, but its function in the enteric pathogens Salmonella enterica and Enterobacter cloacae is unknown. This study aimed to determine the frequency of the LHR in food isolates of E. coli, and its influence on heat resistance in S...
June 2017: Food Microbiology
https://www.readbyqxmd.com/read/28213035/influence-of-electro-activated-solutions-of-weak-organic-acid-salts-on-microbial-quality-and-overall-appearance-of-blueberries-during-storage
#18
Viacheslav Liato, Riadh Hammami, Mohammed Aïder
The aim of this work was to study the potential of diluted electro-activated solutions of weak organic acid salts (potassium acetate, potassium citrate and calcium lactate) to extend the shelf life of blueberries during post-harvest storage. The sanitizing capacity of these solutions was studied against pathogenic bacteria Listeria monocytogenes and E. coli O157:H7 as well as phytopathogenic fungi A. alternata, F. oxysporum and B. cinerea. The results showed that a 5-min treatment of inoculated blueberries with electro-activated solutions resulted in a 4 log CFU/g reduction in Listeria monocytogenes for all solutions...
June 2017: Food Microbiology
https://www.readbyqxmd.com/read/28213028/influence-of-application-sequence-and-timing-of-eugenol-and-lauric-arginate-lae-on-survival-of-spoilage-organisms
#19
Yudith Manrique, Monika Gibis, Herbert Schmidt, Jochen Weiss
The effectiveness of sequential applications of the antimicrobials eugenol and lauric arginate (LAE) was investigated against Staphylococcus carnosus, Listeria innocua, Escherichia coli K12, and Pseudomonas fluorescens. The antimicrobials were applied simultaneously at half of their minimum lethal concentrations (MLC) or sequentially at t = 0 h and t = 3, 4, 6 or 8 h. Bacterial survival was determined by direct plate counts. Survivals kinetic were fitted to a growth and mortality model to obtain characteristic parameters that described time-dependent changes from growth to mortality or vice versa...
June 2017: Food Microbiology
https://www.readbyqxmd.com/read/28213025/influence-of-controlled-atmosphere-on-thermal-inactivation-of-escherichia-coli-atcc-25922-in-almond-powder
#20
Teng Cheng, Rui Li, Xiaoxi Kou, Shaojin Wang
Heat controlled atmosphere (CA) treatments hold potential to pasteurize Salmonella enteritidis PT 30 in almonds. Nonpathogenic Escherichia coli ATCC 25922 was used as a surrogate species of pathogenic Salmonella for validation of thermal pasteurization to meet critical safety requirements. A controlled atmosphere/heating block system (CA-HBS) was used to rapidly determine thermal inactivation of E. coli ATCC 25922. D- and z-values of E. coli ATCC 25922 inoculated in almond powder were determined at four temperatures between 65 °C and 80 °C under different gas concentrations and heating rates...
June 2017: Food Microbiology
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