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Ke-Chuan Wang, Chih-Hung Huang, Shih-Min Ding, Ching-Kuo Chen, Hsu-Wei Fang, Ming-Te Huang, Shiuh-Bin Fang
The yqiC gene of Salmonella enterica serovar Typhimurium (S. Typhimurium) regulates bacterial growth at different temperatures and mice survival after infection. However, the role of yqiC in bacterial colonization and host immunity remains unknown. We infected human LS174T, Caco-2, HeLa, and THP-1 cells with S. Typhimurium wild-type SL1344, its yqiC mutant, and its complemented strain. Bacterial colonization and internalization in the four cell lines significantly reduced on yqiC depletion. Post-infection production of interleukin-8 and human β-defensin-3 in LS174T cells significantly reduced because of yqiC deleted in S...
2016: Frontiers in Microbiology
Bing-Huo Zhang, Nimaichand Salam, Juan Cheng, Han-Quan Li, Jian-Yuan Yang, Dai-Ming Zha, Qi-Gen Guo, Wen-Jun Li
A novel actinobacterium, designated strain JXJ CY 01(T), was isolated from a mucilaginous sheath of Microcystis aeruginosa FACHB-905 collected from Lake Dianchi, south-west China. Taxonomic position of the isolate was determined by polyphasic approaches. Strain JXJ CY 01(T) shared 16S rRNA sequence similarities of 98.9 and 98.0% with Microbacterium marinilacus YM11-607(T) and Microbacterium paludicola US15(T), and less than 98% with other members of the genus Microbacterium. The DNA-DNA relatedness values between strains JXJ CY 01(T) and M...
October 19, 2016: Journal of Antibiotics
Renwen Zhang, Xiaoxue Han, Zhanfeng Xia, Xiaoxia Luo, Chuanxing Wan, Lili Zhang
A novel actinomycete strain, designated TRM 49605(T), was isolated from a desert soil sample from Lop Nur, Xinjiang, north-west China, and characterised using a polyphasic taxonomic approach. The strain exhibited antifungal activity against the following strains: Saccharomyces cerevisiae, Curvularia lunata, Aspergillus flavus, Aspergillus niger, Fusarium oxysporum, Penicillium citrinum, Candida albicans and Candida tropicalis; Antibacterial activity against Bacillus subtilis, Staphylococcus epidermidis and Micrococcus luteus; and no antibacterial activity against Escherichia coli...
October 17, 2016: Antonie Van Leeuwenhoek
Takuya Kaji, Motoki Murai, Hiroaki Itoh, Jyunichiro Yasukawa, Hiroshi Hamamoto, Kazuhisa Sekimizu, Masayuki Inoue
Lysocin E (1) is a structurally complex 37-membered depsipeptide comprising 12 amino-acid residues with an N-methylated amide and an ester linkage. Compound 1 binds to menaquinone (MK) in the bacterial membrane to exert its potent bactericidal activity. To decipher the biologically important functionalities within this unique antibiotic, we performed a comprehensive structure-activity relationship (SAR) study by systematically changing the side-chain structures of l-Thr-1, d-Arg-2, N-Me-d-Phe-5, d-Arg-7, l-Glu-8, and d-Trp-10...
October 14, 2016: Chemistry: a European Journal
Jun Zhang, Xiao-Tong Ma, Jun-Sheng Gao, Cai-Wen Zhang, Juan-Juan Zhao, Rui-Jie Zhang, Li-An Ma, Xiao-Xia Zhang
A novel bacterium, strain 1ZS3-15(T), was isolated from rhizosphere of rice. Its taxonomic position was investigated using a polyphasic approach. The novel strain was observed to be Gram-stain positive, spore-forming, aerobic, motile and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 1ZS3-15(T) was recovered within the genus Paenibacillus. It is closely related to Paenibacillus pectinilyticus KCTC 13222(T) (97.9 % similarity), Paenibacillus frigoriresistens CCTCC AB 2011150(T) (96...
October 12, 2016: Antonie Van Leeuwenhoek
Maria Fusaro, Maurizio Gallieni, Maria Antonietta Rizzo, Andrea Stucchi, Pierre Delanaye, Etienne Cavalier, Rosa M A Moysés, Vanda Jorgetti, Giorgio Iervasi, Sandro Giannini, Fabrizio Fabris, Andrea Aghi, Stefania Sella, Francesco Galli, Valentina Viola, Mario Plebani
Vitamin K (phylloquinone or vitamin K1 and menaquinones or vitamin K2) plays an important role as a cofactor in the synthesis of hepatic blood coagulation proteins, but recently has also aroused an increasing interest for its action in extra-hepatic tissues, in particular in the regulation of bone and vascular metabolism. The accurate measurement of vitamin K status in humans is still a critical issue. Along with indirect assays, such as the undercarboxylated fractions of vitamin K-dependent proteins [prothrombin, osteocalcin (OC), and matrix gla protein], the direct analysis of blood levels of phylloquinone and menaquinones forms might be considered a more informative and direct method for assessing vitamin K status...
October 12, 2016: Clinical Chemistry and Laboratory Medicine: CCLM
Arshia Amin, Iftikhar Ahmed, Nauman Khalid, Ghenijan Osman, Inam Ullah Khan, Min Xiao, Wen-Jun Li
A Gram-staining positive, non-motile, rod-shaped, catalase positive and oxidase negative bacterium, designated NCCP-1331(T), was isolated from a hot water spring soil collected from Tatta Pani, Kotli, Azad Jammu and Kashmir, Pakistan. The isolate grew at a temperature range of 18-40 °C (optimum 30 °C), pH 6.0-9.0 (optimum 7.0) and with 0-6 % NaCl (optimum 2 % NaCl (w/v)). The phylogenetic analysis based on 16S rRNA gene sequence revealed that strain NCCP-1331(T) belonged to the genus Streptomyces and is closely related to Streptomyces brevispora BK160(T) with 97...
October 11, 2016: Antonie Van Leeuwenhoek
Hani M Khojah, Sameh Ahmed, Mahran S Abdel-Rahman, Khaled M Alkhalil, Al-Badr Hamza
The aim of this study was to evaluate the possible role of vitamin K homologs as potential biomarkers for disease activity in patients with rheumatoid arthritis (RA). In this study, 42 patients with RA and 40 healthy controls were enrolled. Serum levels of vitamin K homologs were measured using a high-performance liquid chromatography-fluorescence method. Different biochemical and clinical markers for disease activity were measured and correlated with serum levels of vitamin K homologs. There were no significant differences between RA patients and healthy subjects in demographic data...
October 8, 2016: Journal of Bone and Mineral Metabolism
Luis Franca, Luciana Albuquerque, De-Chao Zhang, Imen Nouioui, Hans-Peter Klenk, Milton S da Costa, Rosa Margesin
A positive Gram-staining, non-motile, catalase-positive and cytochrome c oxidase-negative bacterium, designated strain S20-107T, was isolated from alpine forest soil. Growth occurred at a temperature range of 0-30 °C, at pH 6-9 and in the presence of 0-3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-107T was related to the genus Nakamurella and had the highest 16S rRNA gene sequence similarity with the type strain of Nakamurella flavida DS-52T (96.1 %). Strain S20-107T showed <96...
October 4, 2016: International Journal of Systematic and Evolutionary Microbiology
Sooyeon Park, Min-Ji Ha, Sun Young Yoon, Yong-Taek Jung, Jung-Hoon Yoon
A Gram-stain-negative, non-flagellated, non-gliding, aerobic and rod-shaped bacterium, designated strain OITF-19T, was isolated from a tidal flat in oido, an island of South Korea, and subjected to polyphasic taxonomic study. Strain OITF-19T grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain OITF-19T belonged to the genus Algoriphagus, clustering with the type strain of A. namhaensis showing 96...
September 30, 2016: International Journal of Systematic and Evolutionary Microbiology
Marvin Dörries, Lars Wöhlbrand, Ralf Rabus
The marine sulfate-reducing bacterium Desulfobacterium autotrophicum HRM2 belongs to the deltaproteobacterial family Desulfobacteraceae and stands out for its capacity of facultative chemolithoautotrophic growth (next to heterotrophy). Here, proteomics-driven metabolic reconstruction was based on a combination of 2D DIGE, shotgun proteomics and analysis of the membrane protein-enriched fraction applied to 8 different substrate adaptation conditions (7 aliphatic compounds plus H2 /CO2 ). In total 1,344 different proteins were identified (∼27% of the 4,947 genome-predicted) from which a complex metabolic network was reconstructed consisting of 136 proteins (124 detected; ∼91%)...
October 4, 2016: Proteomics
Long Zhang, Xiao-Long Chen, Qiang Hu, Zhe-Pu Ruan, Kai Chen, Shun-Peng Li, Jian-Dong Jiang
A novel Gram-stain positive, non-motile, rod-shaped bacterial strain designated as LIP-1T was isolated from a contaminated soil of a pesticide factory in Xinyi, China, was investigated for its taxonomic allocation by a polyphasic approach. Cell growth occurred at 16-37 oC (optimum, 30 oC), in the presence of 0-2.0 % (w/v) NaCl (optimum, 0 %) and at pH 6.0-9.0 (optimum, pH 7.0). The major fatty acids of strain LIP-1T were anteiso-C15:0 (50.8 %), iso-C16:0 (17.6 %) and anteiso-C17:0 (17.4 %). The cell-wall peptidoglycan type was B2δ with 2,4-diaminobutyric acid as the diagnostic diamino acid...
September 29, 2016: International Journal of Systematic and Evolutionary Microbiology
David K Cundiff, Paul S Agutter
BACKGROUND: Nutrition researchers recently recognized that deficiency of vitamin K2 (menaquinone: MK-4-MK-13) is widespread and contributes to cardiovascular disease (CVD). The deficiency of vitamin K2 or vitamin K inhibition with warfarin leads to calcium deposition in the arterial blood vessels. METHODS: Using publicly available sources, we collected food commodity availability data and derived nutrient profiles including vitamin K2 for people from 168 countries...
August 24, 2016: Curēus
Sung-Jin Cho, Sang-Seob Lee
A novel Gram-stain positive, aerobic, short rod-shaped, non-motile bacterium, designated strain CHO1(T), was isolated from rhizosphere soil from a ginseng agriculture field. Strain CHO1(T) was observed to form yellow colonies on R2A agar medium. The cell wall peptidoglycan was found to contain alanine, glycine, glutamic acid, D-ornithine and serine. The cell wall sugars were identified as galactose, mannose, rhamnose and ribose. Strain CHO1(T) was found to contain MK-11, MK-12, MK-13 as the predominant menaquinones and anteiso-C15:0, iso-C16:0, and anteiso-C17:0 as the major fatty acids...
September 29, 2016: Antonie Van Leeuwenhoek
Sooyeon Park, Yong-Taek Jung, Sona Kim, Jung-Hoon Yoon
A Gram-stain-negative, facultative aerobic, non-flagellated, and rod-shaped bacterium, designated AR-13(T), was isolated from a seawater on the East Sea in South Korea, and subjected to a polyphasic taxonomic study. Strain AR-13(T) grew optimally at 30°C, at pH 7.0-8.0 and in the presence of 0-0.5% (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain AR-13(T) fell within the clade comprising the type strains of Arcobacter species, clustering coherently with the type strain of Arcobacter venerupis...
October 2016: Journal of Microbiology / the Microbiological Society of Korea
Donyez Frikha-Dammak, Marie-Laure Fardeau, Jean-Luc Cayol, Lilia Ben Fguira-Fourati, Soumaya Najeh, Bernard Ollivier, Sami Maalej
A novel filamentous, halophilic, thermotolerant bacterium, strain SMBg3T was isolated from superficial sediment of a solar saltern in Sfax, Tunisia. The isolate is Gram-staining-positive, aerobic, catalase andoxidase-positive. Its optimum growth occurred at 40-45 °C, 10 % (w/v) NaCl and pH 8.0-9.0. Long and well developed aerial and substrate mycelia, with long chains of fluorescent and circular spores, were observed on all tested media. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SMBg3T belongs to an independent phylogenetic lineage of the family Thermoactinomycetaceae and shows a gene sequence similarity of 94 % with Desmospora activa, 94...
September 25, 2016: International Journal of Systematic and Evolutionary Microbiology
Tong-Wei Guan, Peng-Hao Wang, Lei Tian, Shu-Kun Tang, Hui-Ping Xiang
A novel actinomycete strain, designated XHU 5089T, was isolated from a hypersaline habitat, China. The strain was aerobic, Gram-stain positive and the optimum NaCl concentration for growth was 1-3% (w/v). Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence of strain XHU 5089T showed that the organism was most closely related to Glycomyces albus (97.7%). However, it had relatively low mean DNA-DNA relatedness values with the Glycomyces albus (18.9± 5.8%). The whole-cell sugar pattern consisted of glucose and galactose...
September 25, 2016: International Journal of Systematic and Evolutionary Microbiology
Srinivas A, Divyasree B, Sasikala Ch, Tushar L, Dave Bharti, Ramana Ch V
A Gram-stain-positive, non-motile, rod shaped bacterium (strain JC303T) isolated from a salt pan was identified based on 16S rRNA gene sequence analysis as a member of the genus Jeotgalibacillus. It was most closely related to J. salarius ASL-1T (99.1%), J. alimentarius YKJ-13T (97.9%), J. soli JSM 081008 (97.9%), J. malaysiensis D5T (97.8%), J. marinus DSM 1297T (96.3%), J. campisalis SF-57T (96.1%) and J. soli P9T (94.9%). Genome relatedness based on DNA-DNA hybridization of strain JC303T with the type strains of the closest related species was less than 40%...
September 23, 2016: International Journal of Systematic and Evolutionary Microbiology
Judit Makk, Erika M Tóth, Dóra Anda, Sára Pál, Péter Schumann, Attila L Kovács, Judit Mádl-Szőnyi, Károly Márialigeti, Andrea K Borsodi
Following the exposure of a biofilm sample from a hydrothermal spring cave (Gellért Hill, Budapest, Hungary) to gamma radiation, a strain designated FeSTC15-38T was isolated and studied by polyphasic taxonomic methods. The spherical-shaped cells stained Gram-negative, were aerobic and non-motile. The pH range for growth was 6.0-9.0, with an optimum at pH 7.0. The temperature range for growth was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis based on the 16S rRNA gene sequence of the isolate indicated that the organism belongs to the genus Deinococcus...
September 23, 2016: International Journal of Systematic and Evolutionary Microbiology
Jong-Pyo Kang, Yeon-Ju Kim, Ngoc-Lan Nguyen, Van-An Hoang, Mohamed El-Agamy Farh, Sung-Chul Joo, Lin-Hu Quan, Deok-Chun Yang
Ginseng-cultivated soil is an excellent habitat for soil-borne bacteria to proliferate. A novel strain DCY87T was isolated from ginseng-cultivated soil in Gochang County, Republic of Korea and subsequently characterized by polyphasic approach. Cells were rod-shaped, non-motile, aerobic, Gram-reaction-positive, oxidase-negative and catalase-positive. 16S rRNA gene sequence analysis showed that strain DCY87T shared highest similarity to Phycicoccus ochangensis L1b-b9T (98.7 %). Closely phylogenetic relatives of strain DCY87T were identified: Phycicoccus ginsenosidimutans BXN5-13T (97...
September 23, 2016: International Journal of Systematic and Evolutionary Microbiology
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