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https://www.readbyqxmd.com/read/28714845/bacillus-mangrovi-sp-nov-isolated-from-a-sediment-sample-from-a-mangrove-forest
#1
Vasundhera Gupta, Pradip Kumar Singh, Suresh Korpole, Naga Radha Srinivas Tanuku, Anil Kumar Pinnaka
A facultatively anaerobic, endospore forming, alkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK61T, was isolated from a sediment sample collected from Coringa mangrove forest, India. Colonies were circular, 1.5 mm in diameter, shiny, smooth, yellowish and convex with entire margins after 48 h growth at 30 °C. Growth occurred at 15-42 °C, with 0-3 % (w/v) NaCl and at pH 6-9. AK61T was positive for amylase activity and negative for oxidase, catalase, aesculinase, caseinase, cellulase, DNase, gelatinase, lipase and urease activities...
July 17, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28701039/aminofutalosine-synthase-evidence-for-captodative-and-aryl-radical-intermediates-using-beta-scission-and-srn1-trapping-reactions
#2
Sumedh Joshi, Nilkamal Mahanta, Dmytro Fedoseyenko, Howard J Williams, Tadhg P Begley
Aminofutalosine synthase (MqnE) is a radical SAM enzyme involved in the menaquinone biosynthetic pathway. In this communication, we propose a novel mechanism for this reaction involving the addition of the adenosyl radical to the substrate double bond to form a captodative radical followed by rearrangement and decarboxylation to form an aryl radical anion which is then oxidized by the [4Fe-4S]+2 cluster. Consistent with this proposal, we describe the trapping of the captodative radical and the aryl radical anion using radical triggered C-Br fragmentation reactions...
July 12, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28699880/paenibacillus-konkukensis-sp-nov-isolated-from-animal-feed
#3
Wan-Taek Im, Kwon-Jung Yi, Sang-Suk Lee, Hyung In Moon, Che Ok Jeon, Dong-Woon Kim, Soo-Ki Kim
A Gram-stain-positive, oxidase- and catalase-positive, aerobic, rod-shaped bacterium, designated strain SK-3146T, was isolated from animal feed. Phylogenetic analysis, based on 16S rRNA gene sequence comparisons, revealed that the strain formed a distinct lineage within the genus Paenibacillus that was closely related to Paenibacillusyunnanensis JCM 30953T (98.6 %), Paenibacillusvulneris CCUG 53270T (98.0 %) and Paenibacilluschinjuensis DSM 15045T (96.9 %). Cells were non-motile, endospore-forming and formed milky colonies on NA and R2A agar media...
July 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28699874/algoriphagus-marinus-sp-nov-isolated-from-marine-sediment-and-emended-description-of-the-genus-algoriphagus
#4
Ji-Ru Han, Qing-Li Geng, Feng-Qing Wang, Zong-Jun Du, Guan-Jun Chen
A Gram-stain-negative, facultatively anaerobic coccobacilli approximately 0.4-0.5×0.5-1.1 µm, non-motile, orange-pigmented bacterial strain, designated am2T, was isolated from the coastal area of Weihai, PR China (121° 57' E, 37° 29' N). The temperature, pH and NaCl ranges for growth were 4-40 °C, pH 6.5-8.5 and 0.0-6.0 % (w/v) NaCl. The results of phylogenetic analyses based on 16S rRNA gene sequences revealed that am2T was phylogenetically related to members of the genus Algoriphagus and was closely related to Algoriphagus boritolerans, Algoriphagus namhaensis and Algoriphagus antarcticus with 96...
July 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28699868/flaviramulus-aestuariivivens-sp-nov-isolated-from-a-tidal-flat
#5
Sooyeon Park, Min-Ji Ha, Sun Young Yoon, Jung-Hoon Yoon
A Gram-stain-negative, non-flagellated, non-gliding, aerobic and ovoid or rod-shaped bacterium, designated OITF-51T, was isolated from a tidal flat in Oido, an island of South Korea, and subjected to a polyphasic taxonomic study. Strain OITF-51T grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain OITF-51T forms a cluster with the type strains of three species of the genus Flaviramulus...
July 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28699867/sporolactobacillus-shoreicorticis-sp-nov-a-lactic-acid-producing-bacterium-isolated-from-tree-bark
#6
Vasana Tolieng, Budsabathip Prasirtsak, Mika Miyashita, Chiyo Shibata, Naoto Tanaka, Nuttha Thongchul, Somboon Tanasupawat
A Gram-stain-positive, lactic acid-producing bacterium designed strain MK21-7T, was isolated from tree bark collected from the north east of Thailand. This strain was a facultatively anaerobic spore-forming rod that was catalase-negative. It contained meso-diaminopimelic acid in the cell wall peptidoglycan and had seven isoprene units (MK-7) as the predominant menaquinone. Major fatty acids of MK21-7T were anteiso-C17 : 0, iso-C16 : 0, anteiso-C15 : 0 and C18 : 1ω9c. Polar lipids were phosphatidglycerol, diphosphatidylglycerol, an unknown phospholipid, three unknown glycolipids and an unknown lipid...
July 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28699866/description-of-lysinibacillus-telephonicus-sp-nov-isolated-from-the-screen-of-a-cellular-phone
#7
Praveen Rahi, Rashmi Kurli, Mitesh Khairnar, Shubhangi Jagtap, Aabeejjeet N Pansare, Syed G Dastager, Yogesh S Shouche
A novel bacterial strain, designated S5H2222T, was isolated form the screen of a cellular phone. The cells were Gram-stain-positive, rod-shaped, aerobic and motile, and endospores are formed. S5H2222T grew as pale white colonies on trypticase soy agar and the best growth was observed at 37 °C (10-55 °C) and at pH 7.0 (5.0-9.0). S5H2222T could tolerate up to 10 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences placed this strain within the genus Lysinibacillus and it exhibited high 16S rRNA gene sequence similarity to Lysinibacillus halotolerans LAM612T (97...
July 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28699861/olleya-algicola-sp-nov-a-marine-bacterium-isolated-from-the-green-alga-ulva-fenestrata
#8
Olga I Nedashkovskaya, Song-Gun Kim, Natalia V Zhukova, Valery V Mikhailov
A strictly aerobic, Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 3Alg 18T, was isolated from the Pacific green alga Ulva fenestrata. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain was affiliated to the family Flavobacteriaceae of the phylum Bacteroidetes, being most closely related to the type strains of recognized species of the genus Olleya, with 16S rRNA gene sequence similarity of 97.9-99.3 %. Strain 3Alg 18T grew in the presence of 0...
July 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28699860/leucobacter-corticis-sp-nov-isolated-from-symptomatic-bark-of-populus-%C3%A3-euramericana-canker
#9
Yong Li, Wei Fang, Shou-Jiang Xie, Xuqi Yang, Lai-Fa Wang
A Gram-stain-positive, aerobic, non-motile, rod-shaped bacterial strain, designated 2C-7T, was isolated from symptomatic bark of a Populus × euramericana canker. Growth occurred between 10 and 37 °C and between pH 6 and 10, with optimal growth at 30 °C and pH 7.0-8.0. Growth was present under 0-8 % (w/v) salinity conditions (optimum 1-2 %). Growth occurred in the presence of 10 mM chromium (Cr6+). The major fatty acids (≥10 %) of the novel strain were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0...
July 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28699855/paenibacillus-tritici-sp-nov-isolated-from-wheat-roots
#10
Esther Menéndez, José David Flores-Félix, Rebeca Mulas, Fernando González Andrés, Mercedes Fernández-Pascual, Alvaro Peix, Encarna Velázquez
A bacterial strain designated RTAE36T was isolated from wheat roots in northern Spain. Phylogenetic analyses based on 16S rRNA gene sequence placed the isolate into the genus Paenibacillus with its closest relative being Paenibacillus borealis DSM 13188T with 97.7 % sequence similarity. Cells of the isolate were facultatively anaerobic, Gram-stain-positive, motile and sporulating rods. Catalase and oxidase were positive. Gelatin, casein and starch were not hydrolysed. Growth was supported by many carbohydrates and organic acids as carbon sources...
July 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28699577/sphingobacteriumsoli-sp-nov-isolated-from-soil
#11
Yu-Song Fu, Firasat Hussain, Neeli Habib, Inam Ullah Khan, Xiao Chu, Yan-Qing Duan, Xiao-Yang Zhi, Xing Chen, Wen-Jun Li
A Gram-stain-negative, strictly aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated YIM X0211T, was isolated from a soil sample of Shiling County, Yunnan Province, south-west China. The new isolate was characterized taxonomically by using a polyphasic approach. The strain grew optimally at 30 °C, at pH 7.0 and with 0-3 % (w/v) NaCl. It was positive for catalase and oxidase but negative for H2S production. Comparative 16S rRNA gene sequence analysis showed that strain YIM X0211T fell within the cluster comprising Sphingobacterium species and clustered with Sphingobacterium mizutaii DSM 11724T (97...
July 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28699576/flavobacterium-olei-sp-nov-a-novel-psychrotolerant-bacterium-isolated-from-oil-contaminated-soil
#12
Dhiraj Kumar Chaudhary, Jaisoo Kim
During a study of psychrophilic hydrocarbon-degrading bacteria, two yellow, Gram-staining-negative, non-motile, and rod shaped bacteria, designated R-10-9T and K-4-2 were isolated from oil-contaminated soil from Ulaanbaatar, Mongolia. Both strains were able to grow at 0-32 °C, pH 5.0-9.5, and 0-1 % (w/v) NaCl concentration. These strains were taxonomically characterized by a polyphasic approach. On the basis of the results of 16S rRNA gene sequence analysis, R-10-9T and K-4-2 belong to the genus Flavobacterium and are closely related to Flavobacterium oncorhynchi 631-08T (97...
July 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28698912/spirosoma-areae-sp-nov-isolated-from-soil
#13
Jun Hyeong Jang, Seho Cha, Dongwook Lee, Sathiyaraj Srinivasan, Weonhwa Jheong, Ha Sung Hwang, Myung Kyum Kim, Taegun Seo
A yellow-colored Gram-negative strain, Arct 1-12(T), was isolated from a soil sample collected in Seoul Women's University, South Korea, and grown on R2A agar at 25 °C. Growth of strain Arct 1-12(T) was observed at a temperature range of 15-30 °C (optimal 25 °C), but not at 4 or 42 °C. The strain tolerated up to 1% NaCl (w/v) and displayed optimal growth in the absence of NaCl. Growth occurred at pH 6.0-9.0 (optimally at pH 7). According to the 16S rRNA gene sequence, the strain is moderately related to Spirosoma spitsbergense DSM 19989(T) (93...
July 12, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28698675/virgisporangium-myanmarense-sp-nov-a-novel-motile-actinomycete-isolated-from-an-anthill-soil-in-myanmar
#14
Hideki Yamamura, Shoya Miyazaki, Kodai Ikoma, Youji Nakagawa, Moriyuki Hamada, Misa Otoguro, Tomohiko Tamura, Katsuhiko Ando, Nyunt Phay, Masayuki Hayakawa
An actinomycete strain, designated MM04-1133(T), was isolated from an anthill soil sample collected in Bagan, Myanmar. To establish the taxonomic status of this strain, the isolate was subjected to a polyphasic approach. Strain MM04-1133(T) was Gram-staining positive, aerobic, motile and formed long and narrow sporangia directly above the surface of the substrate mycelium. Whole-cell hydrolysates of the strain contained 3-OH-diaminopimelic acid, arabinose, glucose, galactose, mannose, rhamnose and xylose. The predominant menaquinones were MK-10(H6) and MK-10(H8)...
July 12, 2017: Journal of Antibiotics
https://www.readbyqxmd.com/read/28693664/parafilimonas-rhizosphaerae-sp-nov-isolated-from-the-rhizosphere-of-tomato-plant-solanum-lycopersicum-l
#15
Hayoung Cho, Jae-Hyung Ahn, Hang-Yeon Weon, Jae-Ho Joa, Seung-Beom Hong, Soon-Ja Seok, Jeong-Seon Kim, Soon-Wo Kwon, Soo-Jin Kim
A bacterial strain, designated T16E-198T, was isolated from the rhizosphere of tomato plant collected from a farm on Buyeo-gun, Chungcheongnam-do, South Korea. The strain was aerobic, Gram-stain-negative, rod-shaped, non-flagellated and yellow-pigmented. Strain T16E-198T was mesophilic, catalase- and oxidase-positive and with flexirubin-type pigments. A phylogenetic tree based on 16S rRNA gene sequences showed that strain T16E-198T formed a lineage with Parafilimonas terrae 5GHs7-2T, sharing highest sequence similarity of 98...
July 10, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28691666/hymenobacter-tenuis-sp-nov-isolated-from-wastewater-of-an-acidic-water-neutralization-facility
#16
Joo Won Kang, Ji Hee Lee, Han Na Choe, Chi Nam Seong
A non-motile, red-pink-coloured, rod-shaped bacterium, designated strain POB6T, was isolated from a wastewater treatment facility, Republic of Korea. Cells were Gram-stain-negative, aerobic, catalase-positive and oxidase-negative. The major fatty acids were iso-C15 : 0, C16 : 1ω5c, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B). The strain contained menaquinone MK-7 as the only isoprenoid quinone, phosphatidylethanolamine as the major polar lipid and sym-homospermidine as the major polyamine...
July 8, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28691658/bizionia-sediminis-sp-nov-isolated-from-coastal-sediment
#17
Hui Zhang, Ming-Jing Shi, Hai-Feng Xia, Guan-Jun Chen, Zong-Jun Du
A Gram-stain-negative, aerobic, non-gliding, rod-shaped and orange-coloured bacterium, designated strain P131T, was isolated from marine sediment of the coast of Weihai, China, and subjected to a polyphasic study. Strain P131T was found to grow optimally at 28-30 °C, at pH 7.0-7.5 and in the presence of 2-3 % (w/v) NaCl. In a phylogenetic analysis based on 16S rRNA gene sequences, strain P131T was found to belong to the genus Bizionia and exhibited 94.6-97.0 % 16S rRNA gene sequence similarity with recognized Bizionia species...
July 8, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28686548/roles-of-vitamins-d-and-k-nutrition-and-lifestyle-in-low-energy-bone-fractures-in-children-and-young-adults
#18
Michał Karpiński, Janusz Popko, Katarzyna Maresz, Vladimir Badmaev, Sidney J Stohs
The research on skeletal system health in children and young adults, while recognizing the important role of calcium and vitamin D, goes beyond these nutritional standards. This review focuses on the role of vitamin K in combination with vitamin D and other factors in bone health. The current understanding is that maintaining bone health and prevention of low-energy fractures in any pediatric population includes nutritional factors combined with an active lifestyle. Calcium, vitamin D, and vitamin K supplementation contribute independently and collectively to bone health...
July 2017: Journal of the American College of Nutrition
https://www.readbyqxmd.com/read/28681474/probing-the-menasemiquinone-binding-mode-to-nitrate-reductase-a-by-selective-2h-15n-labelling-hyscore-spectroscopy-and-dft-modeling
#19
Maryam Seif Eddine, Frédéric Biaso, Rodrigo Arias-Cartin, Eric Pilet, Julia Rendon, Sevdalina Lyubenova, Farida Seduk, Bruno Guigliarelli, Axel Magalon, Stephane Grimaldi
In vivo specific isotope labelling at the residue or substituent level is used to probe menasemiquinone (MSK) binding to the quinol oxidation site of respiratory nitrate reductase A (NarGHI) from E. coli. 15N selective labelling of His15Nδ or Lys15Nξ in combination with Hyperfine sublevel correlation, (HYSCORE) spectroscopy unambiguously identified His15N as direct hydrogen bond donor to the radical. In contrast, an essentially anisotropic coupling to Lys15Nξ consistent with a through space magnetic interaction was resolved...
July 5, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28671536/paenibacillus-polysaccharolyticus-sp-nov-a-xylanolytic-and-cellulolytic-bacteria-isolated-from-leaves-of-bamboo-phyllostachys-aureosulcata
#20
Munusamy Madhaiyan, Selvaraj Poonguzhali, Venkatakrishnan Sivaraj Saravanan, Veeramuthu Duraipandiyan, Naif Abdullah Al-Dhabi, Soon-Wo Kwon, William B Whitman
A novel xylanolytic and cellulolytic strain, BL9T, was isolated from leaves of the Bamboo plants maintained at the Tamil Nadu Agricultural University Campus, Coimbatore, India. On the basis of the results of 16S rRNA gene analysis, it was determined to be phylogenetically close to the type strains of Paenibacillus amylolyticus NRRL NRS-290T (98.3 %), Paenibacillus barcinonensis BP-23T (98.1 %), Paenibacillus tundrae A10bT (98.0 %) and Paenibacillus xylanexedens B22aT (97.6 %). The strain stained Gram variable and was aerobic, motile and catalase- and oxidase-positive, with rod-shaped cells...
July 3, 2017: International Journal of Systematic and Evolutionary Microbiology
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