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Liying Jin, Junwei Zhao, Shanwen Jiang, Yang Zhao, Xiaoyang Han, Xiaowei Guo, Xiangjing Wang, Wensheng Xiang
Two Gram-stain positive, aerobic actinomycete strains, designated NEAU-JGR1T and NEAU-JGC41, were isolated from soil collected from Fairy Lake Botanical Garden in Shenzhen, Guangdong Province, south of China. The 16S rRNA gene sequences analysis showed that the two strains exhibited 99.5% 16S rRNA gene sequence similarity with each other and were closely related to Promicromonospora thailandica JCM 17130T (99.4, 99.3%) and Promicromonospora citrea DSM 43110T (99.2, 99.2%). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the two strains clustered together and formed a cluster with P...
May 19, 2018: Antonie Van Leeuwenhoek
Soo-Jeong Lim, Leonid N Ten, Byung-Oh Kim, In-Kyu Kang, Hee-Young Jung
Strain S12-2-1T was isolated from a soil sample collected in the Gyeongsangnam-do province of the Republic of Korea. The isolate is a Gram-stain-negative, aerobic, short, rod-shaped bacterium, and its colonies are red to pink in colour. Analysis of the 16S rRNA gene identified strain S12-2-1T as a member of the genus Hymenobacter in the family Cytophagaceae, with high levels of 16S rRNA gene sequence similarity to Hymenobacter arizonensis OR362-8T (97.7 %), Hymenobacter sedentarius DG5BT (97.4 %) and Hymenobacter humi DG31AT (97...
May 16, 2018: International Journal of Systematic and Evolutionary Microbiology
Salih Saricaoglu, Imen Nouioui, Hilal Ay, Hayrettin Saygin, Kadriye Inan Bektas, Kiymet Guven, Demet Cetin, Hans-Peter Klenk, Kamil Isik, Nevzat Sahin
Strain H2R21T , a novel actinobacterium, isolated from a forest soil sample collected from Heybeliada, Istanbul, Turkey, and a polyphasic approach was used for characterisation of the strain. Chemotaxonomic and morphological characterisation of strain H2R21T indicated that it belongs to the genus Nonomuraea. 16S rRNA gene sequence similarity showed that the strain is closely related to Nonomuraea purpurea 1SM4-01T (99.1%) and Nonomuraea solani CGMCC 4.7037T (98.4%). DNA-DNA relatedness values were found to be lower than 70% between the isolate and its phylogenetic neighbours N...
May 16, 2018: Antonie Van Leeuwenhoek
Dinali Ranmadugala, Alireza Ebrahiminezhad, Merilyn Manley-Harris, Younes Ghasemi, Aydin Berenjian
The effect of milking menaquinone-7 (MK 7) with biocompatible organic solvents on MK 7 production in B. subtilis fermentation was studied. Periodic milking of MK-7 from the fermentation medium by n-hexane significantly enhanced the total MK 7 production ~ 1.7 fold at the end of 72 hours of fermentation (p < 0.05) as compared to the control medium. Milking of MK 7 with a mixture of n-hexane phase modified with n-butanol was also explored. Although milking of MK-7 by a mixture of n-hexane and n-butanol (1:2, v/v), was found to be appropriate in terms of high extraction capacity, no significant increase in total MK-7 concentration was observed...
May 14, 2018: Current Pharmaceutical Biotechnology
Sooyeon Park, Sung-Min Won, Jung-Hoon Yoon
A Gram-stain-negative, aerobic, non-motile, and carrageenan-degrading bacterial strain, designated OISW-17T , was isolated from seawater around Oido, an island of South Korea, and its taxonomic position was determined using a polyphasic study. Optimal growth of the novel strain occurred at 20-25 °C and in the presence of 2.0% (w/v) NaCl. Phylogenetic trees using 16S rRNA gene sequences showed that strain OISW-17T forms a cluster with the type strains of Dokdonia species. The novel strain exhibited 16S rRNA gene sequence similarities of 95...
May 14, 2018: Current Microbiology
Inwoo Baek, Mincheol Kim, Imchang Lee, Seong-In Na, Michael Goodfellow, Jongsik Chun
The genus Turicella was proposed to harbor clinical strains isolated from middle-ear fluids of patients with otitis media. 16S rRNA phylogeny showed that it belonged to the mycolic acid-containing actinobacteria, currently classified in the order Corynebacteriales , and was closely related to the genus Corynebacterium . A new genus was proposed for the organisms as unlike corynebacteria they lacked mycolic acids and had different menaquinones. Here, we carried out large-scale comparative genomics on representative strains of the genera Corynebacterium and Turicella to check if this chemotaxonomic classification is justified...
2018: Frontiers in Microbiology
Koji Mori, Kaoru Yamaguchi, Satoshi Hanada
A novel marine sulfur-oxidizing bacterium, designated strain eps51T , was isolated from a surface rock sample collected from the hydrothermal field of Suiyo Seamount on the Izu-Bonin Arc in the Western Pacific Ocean. This bacterium was Gram-staining-negative, non-motile and rod-shaped. Strain eps51T grew chemolithoautotrophically, by sulfur-oxidizing respiration with elemental sulfur and thiosulfate as electron donors and used only carbon dioxide as a carbon source. Oxygen and nitrate were used as its electron acceptors...
May 14, 2018: International Journal of Systematic and Evolutionary Microbiology
Mustafa Alhaji Isa, Rita Singh Majumdhar, Shazia Haider
The shikimate pathway is as an attractive target because it is present in bacteria, algae, fungi, and plants but does not occur in mammals. In Mycobacterium tuberculosis (MTB), the shikimate pathway is integral to the biosynthesis of naphthoquinones, menaquinones, and mycobactin. In these study, novel inhibitors of 3-dehydroquinate synthase (DHQS), an enzyme that catalyzes the second step of the shikimate pathway in MTB, were determined. 12,165 compounds were selected from two public databases through virtual screening and molecular docking analysis using PyRx 8...
May 11, 2018: Journal of Molecular Modeling
Decai Jin, Xiao Kong, Jie Wang, Jiajun Sun, Xiangyang Yu, Xuliang Zhuang, Ye Deng, Zhihui Bai
A novel Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, strictly aerobic bacterium, designated strain 13T , was isolated from an activated sludge wastewater treatment plant in Beijing, China. 16S rRNA gene sequence analysis placed this organism within the genus Chitinophaga of the class Bacteroidetes, with Chitinophaga skermanii CC-SG1B (92.4 % gene sequence similarity) and Chitinophaga rupis CS5-B1 (91.2 %) as its closest relatives. This isolate contained meso-diaminopimelic acid as the diagnostic diamino acid and the whole-cell hydrolysate was ribose...
May 11, 2018: International Journal of Systematic and Evolutionary Microbiology
Tilak Saha, Biswanath Chakraborty, Sayak Das, Nagendra Thakur, Ranadhir Chakraborty
A Gram-stain-positive, rod-shaped, slightly halotolerant, nitrate-reducing bacterial strain, designated ET03T , was isolated from the cast of an earthworm (Eisenia fetida) reared at the Centre of Floriculture and Agribusiness Management, University of North Bengal at Siliguri, West Bengal, India. On the basis of 16S rRNA gene sequence phylogeny, the closest relative of strain ET03T was Chryseomicrobium palamuruense PU1T (99.1 % similarity). The DNA G+C content of strain ET03T was 42.9 mol%. Strain ET03T contained menaquinone-8 as the most predominant menaquinone and phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and phosphatidylglycerol as the main polar lipids...
May 11, 2018: International Journal of Systematic and Evolutionary Microbiology
Katarzyna Tyśkiewicz, Roman Gieysztor, Izabela Maziarczyk, Paweł Hodurek, Edward Rój, Krystyna Skalicka-Woźniak
In the presented study for the first time a new, optimized, fast SFC (supercritical fluid chromatography) method was applied to separate in one run fat-soluble vitamins from waste fish oil, including cis -and tran s-retinyl palmitate, cis - and trans -retinyl acetate, retinol, &alpha;-tocopherol, &beta;-tocopherol, &gamma;‑tocopherol, &delta;-tocopherol, ergocalciferol (D₂), cholecalciferol (D₃), cis - and trans -phylloquinone (K₁) and menaquinone-4 (K₂-MK4). Vitamins were baseline separated on an Acquity UPC² (ultra performance convergence chromatography) HSS C18 SB (highly strength chemically modified silica) column within 13 min...
May 10, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Junwei Zhao, Shan Mu, Qiannan Zhao, Shanwen Jiang, Peng Cao, Xiaowei Guo, Xiangjing Wang, Wensheng Xiang
A novel actinomycete, designated strain NEAU-mq18T , was isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell. Arg) collected from Xianglu Mountain in Heilongjiang Province, northeast China, and subjected to a polyphasic taxonomic study. The 16S rRNA gene sequence analysis showed that the isolate belongs to the genus Nonomuraea with high sequence similarity to Nonomuraea guangzhouensis NEAU-ZJ3T (98.5%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain clusters phylogenetically with N...
May 9, 2018: Antonie Van Leeuwenhoek
Arkadiusz Szterk, Katarzyna Bus, Adam Zmysłowski, Karol Ofiara
Rapid, global technological development has caused the food industry to use concentrated food component sources like dietary supplements ever more commonly as part of the human diet. This study analysed the menaquinone-7 (MK-7) content of dietary supplements in oil capsule and hard tablet forms. A novel method for separating and measuring geometric isomers of MK-7 in dietary supplements was developed and validated. Eleven different isomers of cis / trans- menaquinone-7 were identified. Identification of cis / trans isomers was performed by combination of HPLC, UPLC and HRMS-QTOF detection, whereas their quantities were determined by DAD detection...
May 1, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Pei Lin, Zheng-Fei Yan, Tae-Hoo Yi
A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium (THG-YT1T ) was isolated from green tea. Growth occurred at 10-40 °C (optimum, 25-30 °C), at pH 6-8 (optimum, 7) and at 0-2 % NaCl (optimum, 0 %). Based on 16S rRNA gene sequences, phylogenetic analyses showed that strain THG-YT1T formed a distinct lineage with respect to closely related genera in the family Bacillaceae. Strain THG-YT1T was most closely related to the genera within the families Pullulanibacillus, Scopulibacillus, Tuberibacillus and Caenibacillu, with levels of 16S rRNA gene sequence similarity to the type species of members of these genera of less than 95...
April 27, 2018: International Journal of Systematic and Evolutionary Microbiology
Heeyoung Kang, Inseong Cha, Haneul Kim, Kiseong Joh
A novel strain, designated HMF3095T , isolated from freshwater of a mesotrophic artificial lake in the Republic of Korea, was characterized by polyphasic taxonomy. The cells were Gram-stain-negative, aerobic, non-motile, straight rods and formed reddish colonies. Phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain HMF3095T fell within the cluster of the genus Hymenobacterand was most closely related to Hymenobacter seoulensis 16F7GT and Hymenobacter tenuis POB6T (96.7 % sequence similarity)...
April 25, 2018: International Journal of Systematic and Evolutionary Microbiology
Bingxue Xu, Beiyu Hu, Jian Wang, Ying Lan, Yaxin Zhu, Xin Dai, Li Huang, Ying Huang, Wenbin Du
Three Gram-variable, moderately halophilic, motile, endospore-forming rods, designated P2-C2T , P3-H5T and P3-B8, were isolated from marine sediment of the Southwest Indian Ocean by using the microfluidic streak plate method. Phylogeny based on 16S rRNA gene sequences showed that strains P2-C2T and P3-H5T formed a robust cluster within the genus Virgibacillus and exhibited 16S rRNA gene similarity levels of 95.3-96.8 and 94.9-96.3 % to the type strains of Virgibacillus species, respectively. The 16S rRNA gene similarity between P2-C2T and P3-H5T was 97...
April 24, 2018: International Journal of Systematic and Evolutionary Microbiology
Yuhang Gao, Chenyu Piao, Han Wang, Linlin Shi, Xiaowei Guo, Jia Song, Wensheng Xiang, Junwei Zhao, Xiangjing Wang
A novel actinomycete, designated strain NEAU-G57T was isolated from a soil sample collected from the bottom of a river in Chad. A polyphasic approach was employed to determine the status of strain NEAU-G57T . Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the organism should be assigned to the genus Pseudonocardia and formed a monophyletic clade with its closest relatives Pseudonocardia yuanmoensis YIM 75926T (98.8 %), Pseudonocardia halophobica DSM 43089T (98.2 %) and Pseudonocardia kujensis A 4038T (97...
April 23, 2018: International Journal of Systematic and Evolutionary Microbiology
Chun-Gon Kim, Jong-Pyo Kang, Yue Huo, Mohan Chokkalingam, Yeon-Ju Kim, Dong-Hyun Kim, Deok-Chun Yang
A novel bacterium, designated DCY114T , was isolated from ginseng-cultivated soil in Gochang-gun, Republic of Korea. This isolate was assigned to the genus Paenibacillus and is closely related to Paenibacillus amylolyticus NRRL NRS-290T (98.3%), P. dongdonensis KUDC0114T (98.0%), P. tylopili MK2T (97.9%), P. tundrae A10bT (97.8%), and P. xylanexedens B22aT (97.5%) based on 16S rRNA gene sequence analysis. Strain DCY114T is a Gram-reaction positive, catalase and oxidase positive, facultatively aerobic rod that is motile by peritrichous flagella...
April 20, 2018: Archives of Microbiology
Shuai-Bo Han, Yang-Huan Yu, Zhao Ju, Yu Li, Ran Zhang, Xin-Jun Hou, Xin-Yuan Ma, Xiao-Yun Yu, Cong Sun, Min Wu
A Gram-stain-negative, non-motile, aerobic, rod-shaped bacterium, designated 15182T , was isolated from a saline lake in China. The novel strain 15182T was able to grow at 10-40 °C (optimum, 37 °C), pH 7.0-8.0 (optimum, 7.5) and with 0.5-4 % NaCl (optimum, 2-3 %, w/v). The phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 15182T was most closely related to the genus Rhodohalobacter by sharing the highest sequence similarity of 97.0 % with Rhodohalobacter halophilus JZ3C29T ...
April 20, 2018: International Journal of Systematic and Evolutionary Microbiology
Yunho Lee, Che Ok Jeon
A novel orange-pigmented, Gram-stain-negative and strictly aerobic bacterium, designated strain HR-UT , was isolated from a water sample of the Han River located in the Republic of Korea. Cells were catalase-positive and oxidase-positive, and non-motile curved rods without flagella. The strain grew at 5-35 °C (optimum 25 °C), pH 6-10 (optimum of 7-8) and 0-3 % (w/v) NaCl (optimum 0 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HR-UT formed a tight phylogenic lineage with Siphonobacter aquaeclarae DSM 21668T and 'Siphonobacter intestinalis' 63MJ-2...
April 20, 2018: International Journal of Systematic and Evolutionary Microbiology
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