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https://www.readbyqxmd.com/read/27906439/in-vitro-neural-differentiation-of-bone-marrow-stromal-cells-induced-by-hepatocyte-growth-factor-and-glial-cell-derived-neurotrophic-factor
#1
Q Ma, M Cai, J-W Shang, J Yang, X-Y Gu, W-B Liu, Q Yang
OBJECTIVE: Bone marrow stromal cells (BMSCs) have great potential for cell-based transplantation therapy in treating neurological disease. However, the best combination of various trophic factors to produce full neural differentiation of BMSCs was still unclear. In our study, we aimed to investigate the neural differentiation capacity of rat BMSCs induced by growth factors including hepatocyte growth factor (HGF) and glial cell-derived neurotrophic factor (GDNF). MATERIALS AND METHODS:   Cell counting kit-8 (CCK-8) assay, BrdU cell proliferation assay and flow cytometry were implemented to evaluate whether GDNF and HGF had positive effects on the proliferation of BMSCs...
November 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/27889959/fumaric-acid-esters-attenuate-secondary-degeneration-following-spinal-cord-injury
#2
Marika Cordaro, Giovanna Casili, Irene Paterniti, Salvatore Cuzzocrea, Emanuela Esposito
Spinal cord injury (SCI) causes permanent changes in motor, sensory and autonomic functions. Unfortunately, there are not a stable cures and current treatments include surgical decompression, methylprednisolone and hemodynamic control that lead to modest function recovery. Fumaric acid esters (FAEs) were firstly used in the management of an immunological skin disorder, such as psoriasis. Because of their potent anti-inflammatory effects, they have been introduced in multiple sclerosis. Investigation shown not only anti-inflammatory, but also supposed neuroprotective mechanism of action...
November 27, 2016: Journal of Neurotrauma
https://www.readbyqxmd.com/read/27888042/changes-in-neurotrophic-and-inflammatory-factors-in-the-cerebrospinal-fluid-of-patients-with-postherpetic-neuralgia
#3
Wenxing Zhao, Yong Wang, Qiwu Fang, Jianping Wu, Xinyou Gao, Hui Liu, Liu Cao, Jianxiong An
Inflammatory and neurotrophic factors are involved in postherpetic neuralgia (PHN), but the association of these factors in the cerebrospinal fluid (CSF) with the level of pain is poorly known. The present study aimed to examine the changes in neurotrophic and inflammatory factors in the CSF of patients with PHN and to study the correlation between these factors and the degree of pain. Fifty patients with PHN and 28 patients with hemifacial spasm (as controls) were recruited between May 2015 and March 2016...
November 23, 2016: Neuroscience Letters
https://www.readbyqxmd.com/read/27884342/optimizing-neurogenic-potential-of-enteric-neurospheres-for-treatment-of-neurointestinal-diseases
#4
Lily S Cheng, Hannah K Graham, Wei Hua Pan, Nandor Nagy, Alfonso Carreon-Rodriguez, Allan M Goldstein, Ryo Hotta
BACKGROUND: Enteric neurospheres derived from postnatal intestine represent a promising avenue for cell replacement therapy to treat Hirschsprung disease and other neurointestinal diseases. We describe a simple method to improve the neuronal yield of spontaneously formed gut-derived neurospheres. MATERIALS AND METHODS: Enteric neurospheres were formed from the small and large intestines of mouse and human subjects. Neurosphere size, neural crest cell content, cell migration, neuronal differentiation, and neuronal proliferation in culture were analyzed...
December 2016: Journal of Surgical Research
https://www.readbyqxmd.com/read/27877111/polymerizing-pyrrole-coated-poly-l-lactic-acid-co-%C3%AE%C2%B5-caprolactone-plcl-conductive-nanofibrous-conduit-combined-with-electric-stimulation-for-long-range-peripheral-nerve-regeneration
#5
Jialin Song, Binbin Sun, Shen Liu, Wei Chen, Yuanzheng Zhang, Chunyang Wang, Xiumei Mo, Junyi Che, Yuanming Ouyang, Weien Yuan, Cunyi Fan
Electrospinning and electric stimulation (ES) are both promising methods to support neuron adhesion and guide extension of neurons for nerve regeneration. Concurrently, all studies focus on either electrospinning for conduits material or ES in vitro study to accelerate nerve regeneration; few work on the combined use of these two strategies or ES in vivo study. Therefore, this study aimed to investigate the abilities of direct current ES through electrospinning conductive polymer composites composed of polypyrrole and Poly (l-lactic acid-co-ε-caprolactone) (PPY/PLCL) in peripheral nerve regeneration...
2016: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/27874834/recovery-of-erectile-function-comparing-autologous-nerve-grafts-unseeded-conduits-schwann-cell-seeded-guidance-tubes-and-gdnf-overexpressing-schwann-cell-grafts
#6
Florian May, Alexander Buchner, Kaspar Matiasek, Boris Schlenker, Christian Stief, Norbert Weidner
Dissection of the cavernous nerves during radical prostatectomy for prostate cancer eliminates spontaneous erections. Using the rat as an experimental model, we compared the regenerative capacity of autologous nerve grafts and Schwann cell seeded nerve guides.After bilateral excision of cavernous nerve segments, cavernous nerves were reconstructed using unseeded silicon tubes (UT), nerve autografts (NA) and silicon tubes seeded with either Glial cell line-derived (GDNF)-overexpressing or green fluorescent protein (GFP)-expressing Schwann cells (SCs) (16 study nerves per group)...
November 17, 2016: Disease Models & Mechanisms
https://www.readbyqxmd.com/read/27867665/dose-dependent-differential-effect-of-neurotrophic-factors-on-in-vitro-and-in-vivo-regeneration-of-motor-and-sensory-neurons
#7
Daniel Santos, Francisco Gonzalez-Perez, Xavier Navarro, Jaume Del Valle
Although peripheral axons can regenerate after nerve transection and repair, functional recovery is usually poor due to inaccurate reinnervation. Neurotrophic factors promote directional guidance to regenerating axons and their selective application may help to improve functional recovery. Hence, we have characterized in organotypic cultures of spinal cord and dorsal root ganglia the effect of GDNF, FGF-2, NGF, NT-3, and BDNF at different concentrations on motor and sensory neurite outgrowth. In vitro results show that GDNF and FGF-2 enhanced both motor and sensory neurite outgrowth, NGF and NT-3 were the most selective to enhance sensory neurite outgrowth, and high doses of BDNF selectively enhanced motor neurite outgrowth...
2016: Neural Plasticity
https://www.readbyqxmd.com/read/27864362/identification-of-lysophosphatidic-acid-receptor-1-in-astroglial-cells-as-a-target-for-glial-cell-line-derived-neurotrophic-factor-expression-induced-by-antidepressants
#8
Naoto Kajitani, Kanako Miyano, Mami Okada-Tsuchioka, Hiromi Abe, Kei Itagaki, Kazue Hisaoka-Nakashima, Norimitsu Morioka, Yasuhito Uezono, Minoru Takebayashi
Preclinical and clinical evidence suggests that glial cell line-derived neurotrophic factor (GDNF) is important in the therapeutic effect of antidepressants. A previous study demonstrated that the tricyclic antidepressant amitriptyline induces Gαi/o activation, which leads to GDNF expression in astrocytes. However, the specific target expressed in astrocytes that mediates antidepressant-evoked Gαi/o activation has yet to be identified. Thus, the current study explored the possibility that antidepressant-induced Gαi/o activation depends on lysophosphatidic acid receptor 1 (LPAR1), a Gαi/o-coupled receptor...
November 18, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27862224/escalating-methamphetamine-regimen-induces-compensatory-mechanisms-mitochondrial-biogenesis-and-gdnf-expression-in-substantia-nigra
#9
Neda Valian, Abolhassan Ahmadiani, Leila Dargahi
Methamphetamine (MA) produces long-lasting deficits in dopaminergic neurons in the long-term use via several neurotoxic mechanisms. The effects of MA on mitochondrial biogenesis is less studied currently. So, we evaluated the effects of repeated escalating MA regimen on transcriptional factors involved in mitochondrial biogenesis and glial-derived neurotrophic factor (GDNF) expression in substantia nigra (SN) and striatum of rat. In male wistar rats, increasing doses of MA (1-14 mg/kg) were administrated twice a day for 14 days...
November 15, 2016: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/27844282/morphological-changes-in-a-severe-model-of-parkinson-s-disease-and-its-suitability-to-test-the-therapeutic-effects-of-microencapsulated-neurotrophic-factors
#10
C Requejo, J A Ruiz-Ortega, H Bengoetxea, A García-Blanco, E Herrán, A Aristieta, M Igartua, J L Pedraz, L Ugedo, R M Hernández, J V Lafuente
The unilateral 6-hydroxydopamine (6-OHDA) lesion of medial forebrain bundle (MFB) in rats affords us to study the advanced stages of Parkinson's disease (PD). Numerous evidences suggest synergic effects when various neurotrophic factors are administered in experimental models of PD. The aim of the present work was to assess the morphological changes along the rostro-caudal axis of caudo-putamen complex and substantia nigra (SN) in the referred model in order to test the suitability of a severe model to evaluate new neurorestorative therapies...
November 14, 2016: Molecular Neurobiology
https://www.readbyqxmd.com/read/27836239/atypical-antipsychotic-treatment-increases-glial-cell-line-derived-neurotrophic-factor-serum-levels-in-drug-free-schizophrenic-patients-along-with-improvement-of-psychotic-symptoms-and-therapeutic-effects
#11
Wenhuan Xiao, Fei Ye, Li Ma, Xiaowei Tang, Jin Li, Hui Dong, Weiwei Sha, Xiaobin Zhang
Glial cell line-derived neurotrophic factor (GDNF) plays an increasingly vital role in the pathogenesis of neuropsychiatric illnesses. Antipsychotic medications were shown to stimulate GDNF secretion from C6 glioma cells. The aims of this study were to investigate the serum concentration of GDNF, to monitor the therapeutic effect of atypical antipsychotics related to GDNF levels in drug-free schizophrenia patients, and to examine these levels in relation to psychotic symptoms. We recruited 138 drug-free schizophrenic patients and compared them with 77 matched healthy subjects...
November 3, 2016: Psychiatry Research
https://www.readbyqxmd.com/read/27827452/gdnf-expressing-sto-feeder-layer-supports-the-long-term-propagation-of-undifferentiated-mouse-spermatogonia-with-stem-cell-properties
#12
Xiang Wei, Yuanyuan Jia, Yuanyuan Xue, Lei Geng, Min Wang, Lufan Li, Mei Wang, Xuemei Zhang, Xin Wu
The development of a stem cell culture system would expedite our understanding of the biology of tissue regeneration. Spermatogonial stem cell (SSC) is the foundation for lifelong male spermatogenesis and the SSC culture has been optimized continuously in recent years. However, there have been many inconveniences to reconstruct SSC self-renewal and proliferation in vitro, such as the frequent refreshment of recombinant cytokines, including GDNF, the essential growth factor for SSC maintenance. In the present study, we observed that both STO and MEF cells, which were previously used as feeders for SSC growth, did not express GDNF, but a GDNF-expressing STO feeder could support undifferentiated mouse spermatogonia propagation in vitro for three months without the refreshment of recombinant growth factor GDNF...
November 9, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27825404/comparsion-between-intravenous-delivered-human-fetal-bone-marrow-br-mesenchymal-stromal-cells-and-mononuclear-cells-in-br-the-treatment-of-rat-cerebral-infarct
#13
Ai-Hua Huang, Ping-Ping Zhang, Bin Zhang, Bu-Qing Ma, Yun-Qian Guan, Yi-Dan Zhou
Objective To compare the effecacy of human mesenchymal stromal cell (hMSC) with human mononuclear cell (hMNC) in treating rat cerebral infarct.Methods The SD rat models of cerebral infarct were established by distal middle cerebral artery occlusion (dMCAO). Rats were divided into four groups: sham,ischemia vehicle,MSC,and MNC transplantation groups. For the transplantation group,1×10(6) hMSCs or hMNCs were intravascularly transplanted into the tail vein 1 hour after the ischemia onset. The ischemia vehicle group received dMCAO surgery and intravascular saline injection 1,3,5,and 7 days after the ischemia onset,and then behavioral tests were performed...
October 10, 2016: Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
https://www.readbyqxmd.com/read/27813586/effects-of-glial-cell-line-derived-neurotrophic-factor-and-leukemia-inhibitory-factor-on-the-behavior-of-two-calf-testis-germline-stem-cell-colony-types
#14
P Zheng, H Huang, Y G Tian, Y Zeng, E O Adegoke, X Wang, G X Zhang
Germline stem cells are the only such capable of transmitting genetic information in vivo. The isolation and culture of these cells in vitro provide a unique model to understand sperm differentiation and hence, spermatogenesis and male fertility. In this study, we isolated, purified, and cultured germline stem cells from the testes of newborn calves. Moreover, we investigated the effects of glial cell line-derived neurotrophic factor (GDNF) and leukemia-inhibitory factor (LIF) on their proliferation. Male calf germline stem cells were found to be pluripotent, and able to form grape-like and embryonic stem cell (ES)-like colonies when cultured...
October 17, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27803784/the-expression-implication-of-gdnf-in-ventral-horn-and-associated-remote-cortex-in-rhesus-monkeys-with-hemisected-spinal-cord-injury
#15
De-Lu Qiu, Ting-Hua Wang
OBJECTIVES: Glial cell line-derived neurotrophic factor (GDNF) can effectively promote axonal regeneration, limit axonal retraction, and produce a statistically significant improvement in motor recovery after spinal cord injury (SCI). However, the role in primate animals with SCI is not fully cognized. MATERIALS AND METHODS: 18 healthy juvenile rhesuses were divided randomly into six groups, observed during the periods of 24 hr, 7 days, 14 days, 1 month, 2 months, and 3 months after T11 hemisecting...
September 2016: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/27796870/gpr30-activation-contributes-to-the-puerarin-mediated-neuroprotection-in-mpp-induced-sh-sy5y-cell-death
#16
Yue-Fa Cheng, Guoqi Zhu, Qing-Wen Wu, Yue-Sheng Xie, Yan Jiang, Lan Guo, Ya-Li Guan, Ying-Shuo Liu, Jun Zhang
The neuroprotective action of puerarin in Parkinson's disease (PD) models has been well investigated. However, the mechanisms involved in protection have not been completely understood. G protein-coupled receptor 30 (GPR30) is a G protein-coupled estrogen receptor and considered a potential target in the neuroprotection against PD. In this study, we investigated whether puerarin prevented against 1-methyl-4-phenylpyridinium (MPP(+))-induced cell death via GPR30. Our results showed that the GPR30 agonist, G1, exhibited puerarin-mediated neuroprotection against MPP(+)-induced cell death of SH-SY5Y cells...
October 30, 2016: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/27778122/fk962-induces-neurite-outgrowth-in-cultured-monkey-trigeminal-ganglion-cells
#17
Emi Nakajima, Ryan D Walkup, Thomas R Shearer, Mitsuyoshi Azuma
PURPOSE: Corneal sensation, cell proliferation, and wound healing all depend on adequate corneal innervation. Disruption of corneal innervation can lead to dry eye and delayed wound healing. Our studies in rats and rabbits show that the substituted fluorobenzamide drug FK962 accelerates the extension of neuronal processes and recovery of corneal sensitivity. The purpose of the present study was 1) to determine whether FK962 induces sprouting and elongation of neurites in cultured monkey trigeminal ganglion cells, and 2) to investigate the involvement of the neurotrophic peptide GDNF in FK962-induced neurite elongation...
October 24, 2016: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/27775476/alteration-of-selected-neurotrophic-factors-and-their-receptor-expression-in-mouse-brain-response-to-whole-brain-irradiation
#18
Ewa Pius-Sadowska, Miłosz Piotr Kawa, Patrycja Kłos, Dorota Rogińska, Michał Rudnicki, Boehlke Marek, Piotr Waloszczyk, Bogusław Machaliński
Ionizing radiation can significantly affect brain function in children and young adults, particularly in the hippocampus where neurogenic niches are located. Injury to normal tissue is a major concern when whole-brain irradiation (WBI) is used to treat central nervous system (CNS) tumors, and the pathogenesis of this injury remains poorly understood. We assessed the expression of selected neurotrophins (NTs) and NT receptors (NTRs) in brains of young mice after a single 10 Gy gamma-ray exposure using morphological and molecular analyses [qRT-PCR, Western blot, immunohistochemistry (IHC)] to evaluate WBI-induced injury in its acute phase...
October 24, 2016: Radiation Research
https://www.readbyqxmd.com/read/27772714/necl1-coated-plga-as-favorable-conduits-for-repair-of-injured-peripheral-nerve
#19
Fuben Xu, Kun Zhang, Peizhen Lv, Rongbin Lu, Li Zheng, Jinmin Zhao
Restoration of normal neurological function of transected peripheral nerve challenged regenerative medicine and surgery. Previous studies showed that Nectin-like molecule 1 (NECL1) is one of the important adhesion molecules on the axons and Schwann cells is located along the internodes in direct apposition to NECL1. In this study, we fabricated PLGA membrane pre-coated with NECL1, mimicking the natural axons to enhance the adhesion of Schwann cells. Investigation of the cellular response in vitro was performed by detecting cytotoxicity, proliferation, morphology, viability, specific markers and Scanning Electron Microscopy (SEM) of Schwann cells cultured in PLGA...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27771993/identification-prokaryote-expression-of-medaka-gdnfa-b-and-their-biological-activity-in-a-spermatogonial-cell-line
#20
Jing Wei, Linyan Liu, Zhenhua Fan, Yunhan Hong, Yang Zhao, Linyan Zhou, Deshou Wang
The origin and evolution of molecular mechanisms underlying the self-renewal and differentiation of spermatogonial stem cells (SSC) are fundamental questions in stem cell biology as well as reproduction medicine. In mammals, glial cell line-derived neurotrophic factor (GDNF) is crucial for SSC self-renewal and maintenance. However, in non-mammals, the role of Gdnf in SSC still remains unknown. Here we report that the two GDNF homologs from medaka fish (Oryzias latipes), namely OlGdnfa and OlGdnfb, can promote the proliferation activity and retain the spermatogonial property of SG3, a spermatogonial cell line derived from adult medaka showing the intrinsic property of SSC by self-renewal and differentiation potential during 2 years of culture...
October 22, 2016: Stem Cells and Development
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