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https://www.readbyqxmd.com/read/28324850/sludge-management-modeling-to-enhance-p-recovery-as-struvite-in-wastewater-treatment-plants
#1
N Martí, R Barat, A Seco, L Pastor, A Bouzas
Interest in phosphorus (P) recovery and reuse has increased in recent years as supplies of P are declining. After use, most of the P remains in wastewater, making Wastewater Treatment Plants (WWTPs) a vital part of P recycling. In this work, a new sludge management operation was studied by modeling in order to recover P in the form of struvite and minimize operating problems due to uncontrolled P precipitation in WWTPs. During the study, intensive analytical campaigns were carried out on the water and sludge lines...
March 15, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28324833/simplified-purification-approach-of-urinary-neutrophil-gelatinase-associated-lipocalin-by-tangential-flow-filtration-and-ion-exchange-chromatography
#2
Kunal K Shukla, Shamkant B Badgujar, Paresh B Bhanushali, Sushma G Sabharwal
This investigation reports a simplified approach for the purification of urinary siderocalin known as neutrophil gelatinase-associated lipocalin (NGAL). Urinary NGAL was purified by tangential flow filtration and ion exchange chromatography. Isolated NGAL was analyzed by SDS-PAGE, immunoblotting and mass spectrometry (MS). The relative molecular mass of NGAL is 23674Da. Peptide mass fingerprinting of the purified NGAL yielded peptides that partially matched with known sequence of P80188 (NGAL_HUMAN). The tryptic digestion profile of isolated NGAL infers that it may be unique and additive molecule in the dictionary of urinary proteins...
March 11, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28324712/comparison-of-gaseous-polycyclic-aromatic-hydrocarbon-metabolites-according-to-their-specificity-as-biomarkers-of-occupational-exposure-selection-of-2-hydroxyfluorene-and-2-hydroxyphenanthrene
#3
Damien Barbeau, Simon Lutier, Marie Marques, Renaud Persoons, Anne Maitre
Exposure to Polycyclic Aromatic Hydrocarbons (PAHs) occurs by respiratory, digestive and dermal absorption. Biomonitoring takes all pathways into account but sensitive and specific biomarkers are required. Different gaseous PAHs metabolites were used due to their abundance in the atmospheric mixtures but none of them were selected as better biomarker than the others. To identify the best candidates for assessing occupational airborne exposure, relation between atmospheric levels of Naphtalene, Fluorene and Phenanthrene and urinary metabolites concentrations was studied in a carbon electrode workers group...
March 6, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28324497/telomere-g-rich-overhang-length-measurement-dsn-method
#4
Yong Zhao, Jerry W Shay, Woodring E Wright
Telomeres terminate in 3' single-stranded G-rich overhangs that function in telomere end protection and telomerase action. An accurate measurement of overhang length is challenging due to the presence of many kilobases of double-stranded telomere DNA. Here, a simple method is described that utilizes duplex specific nuclease (DSN) to digest all double-stranded DNA including telomeres to fragments of <10 bp, leaving the single-stranded overhangs intact. Their length can then be determined by Southern blot using super-sensitive telomere C-rich probes...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28324211/comparison-of-in-vitro-osteogenic-potential-of-iliac-crest-and-degenerative-facet-joint-bone-autografts-for-intervertebral-fusion-in-lumbar-spinal-stenosis
#5
Jeroen Geurts, Daniela Ramp, Stefan Schären, Cordula Netzer
PURPOSE: The promotion of spinal fusion using bone autografts is largely mediated by the osteoinductive potential of progenitors/mesenchymal stem cells (MSC) that reside in the marrow spaces of cancellous bone. Iliac crest is the common autograft donor site, but its use presents an increased risk for donor site pain, morbidity and infection. Degenerative bone samples harvested during facetectomy might provide an alternative viable source of osteoinductive autografts. In this study, we conducted an intra-individual comparison of the osteogenic potential of isolated low passage MSC from both sources...
March 21, 2017: European Spine Journal
https://www.readbyqxmd.com/read/28324209/molecular-cloning-structural-modeling-and-the-production-of-soluble-triple-mutated-diphtheria-toxoid-k51e-g52e-e148k-co-expressed-with-molecular-chaperones-in-recombinant-escherichia-coli
#6
Naphatsamon Uthailak, Pornpimol Mahamad, Pamorn Chittavanich, Somchai Yanarojana, Wassana Wijagkanalan, Jean Petre, Watanalai Panbangred
CRM197 is a diphtheria toxin (DT) mutant (G52E) which has been used as a carrier protein for conjugate vaccines. However, it still possesses cytotoxicity toward mammalian cells. The goal of this project was to produce a non-toxic and soluble CRM197EK through introduction of triple amino acid substitutions (K51E/G52E/E148K) in Escherichia coli. The expression of CRM197EKTrxHis was optimized and co-expressed with different molecular chaperones. The soluble CRM197EKTrxHis was produced at a high concentration (97...
March 21, 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/28323908/effects-of-protease-and-non-starch-polysaccharide-enzyme-on-performance-digestive-function-activity-and-gene-expression-of-endogenous-enzyme-of-broilers
#7
Lin Yuan, Mingfa Wang, Xiaotu Zhang, Zhixiang Wang
Three hundred one-day-old male broiler chickens (Ross-308) were fed corn-soybean basal diets containing non-starch polysaccharide (NSP) enzyme and different levels of acid protease from 1 to 42 days of age to investigate the effects of exogenous enzymes on growth performance, digestive function, activity of endogenous digestive enzymes in the pancreas and mRNA expression of pancreatic digestive enzymes. For days 1-42, compared to the control chickens, average daily feed intake (ADFI) and average daily gain (ADG) were significantly enhanced by the addition of NSP enzyme in combination with protease supplementation at 40 or 80 mg/kg (p<0...
2017: PloS One
https://www.readbyqxmd.com/read/28323901/characterization-of-stromal-vascular-fraction-and-adipose-stem-cells-from-subcutaneous-preperitoneal-and-visceral-morbidly-obese-human-adipose-tissue-depots
#8
Karina Ribeiro Silva, Isis Côrtes, Sally Liechocki, João Regis Ivar Carneiro, Antônio Augusto Peixoto Souza, Radovan Borojevic, Clarissa Menezes Maya-Monteiro, Leandra Santos Baptista
BACKGROUND/OBJECTIVES: The pathological condition of obesity is accompanied by a dysfunctional adipose tissue. We postulate that subcutaneous, preperitoneal and visceral obese abdominal white adipose tissue depots could have stromal vascular fractions (SVF) with distinct composition and adipose stem cells (ASC) that would differentially account for the pathogenesis of obesity. METHODS: In order to evaluate the distribution of SVF subpopulations, samples of subcutaneous, preperitoneal and visceral adipose tissues from morbidly obese women (n = 12, BMI: 46...
2017: PloS One
https://www.readbyqxmd.com/read/28323486/enterolactone-induces-g1-phase-cell-cycle-arrest-in-nonsmall-cell-lung-cancer-cells-by-downregulating-cyclins-and-cyclin-dependent-kinases
#9
Shireen Chikara, Kaitlin Lindsey, Harsharan Dhillon, Sujan Mamidi, Jeffrey Kittilson, Melpo Christofidou-Solomidou, Katie M Reindl
Flaxseed is a rich source of the plant lignan secoisolariciresinol diglucoside (SDG), which is metabolized into mammalian lignans enterodiol (ED) and enterolactone (EL) in the digestive tract. The anticancer properties of these lignans have been demonstrated for various cancer types, but have not been studied for lung cancer. In this study, we investigated the anticancer effects of EL for several nonsmall cell lung cancer (NSCLC) cell lines of various genetic backgrounds. EL inhibited the growth of A549, H441, and H520 lung cancer cells in concentration- and time-dependent manners...
March 21, 2017: Nutrition and Cancer
https://www.readbyqxmd.com/read/28323452/how-to-manage-plant-biomass-originated-from-phytotechnologies-gathering-perceptions-from-end-users
#10
V Bert, S Neu, I Zdanevitch, W Friesl-Hanl, S Collet, R Gaucher, M Puschenreiter, I Müller, J Kumpiene
A questionnaire survey was carried out in 4 European countries to gather end-user's perceptions of using plants from phytotechnologies in combustion and anaerobic digestion (AD). 9 actors of the wood energy sector from France, Germany and Sweden, and 11 AD platform operators from France, Germany and Austria were interviewed. Questions related to installation, input materials, performed analyses, phytostabilization and phytoextraction. Although the majority of respondents did not know phytotechnologies, results suggested that plant biomass from phytomanaged areas could be used in AD and combustion, under certain conditions...
March 21, 2017: International Journal of Phytoremediation
https://www.readbyqxmd.com/read/28323417/construction-of-a-database-of-collision-cross-section-values-for-glycopeptides-glycans-and-peptides-determined-by-im-ms
#11
Rebecca S Glaskin, Kshitij Khatri, Qi Wang, Joseph Zaia, Catherine E Costello
An ion mobility quadrupole time-of-flight mass spectrometer was used to examine the gas-phase structures of a set of glycopeptides resulting from proteolytic digestion of the well-characterized glycoproteins bovine ribonuclease B, human transferrin, bovine fetuin and human α1-acid glycoprotein, the corresponding deglycosylated peptides and the glycans released by the endoglycosidase PNGase F. When closely related glycoforms did not occur naturally, exoglycosidases were used to achieve stepwise removal of individual saccharide units from the non-reducing termini of the multi-antennary structures...
March 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28320060/clinical-value-of-octamer-binding-transcription-factor-4-as-a-prognostic-marker-in-patients-with-digestive-system-cancers-a-systematic-review-and-meta-analysis
#12
Zhiqiang Chen, Long Zhang, Qin Zhu, Xiaowei Wang, Jindao Wu, Xuehao Wang
BACKGROUND AND AIM: The role of octamer-binding transcription factor 4 (Oct4) has been implicated in the clinical prognosis of various kinds of digestive system cancers, but the results remain controversial. The purpose of this meta-analysis is to assess the potential role of Oct4 as a prognostic marker in digestive system tumors. METHODS: Relevant articles were retrieved from Pubmed, Web of Science, and Cochrane Library up to July 2016. The software Stata 12.0 was used to analyze the outcomes, including overall survival (OS), disease-free survival, recurrence-free survival, and clinicopathological characteristics...
March 2017: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28319893/role-of-macrophages-in-age-related-oxidative-stress-and-lipofuscin-accumulation-in-mice
#13
Carmen Vida, Irene Martínez de Toda, Julia Cruces, Antonio Garrido, Mónica Gonzalez-Sanchez, Mónica De la Fuente
The age-related changes in the immune functions (immunosenescence) may be mediated by an increase of oxidative stress and damage affecting leukocytes. Although the "oxidation-inflammation" theory of aging proposes that phagocytes are the main immune cells contributing to "oxi-inflamm-aging", this idea has not been corroborated. The aim of this work was to characterize the age-related changes in several parameters of oxidative stress and immune function, as well as in lipofuscin accumulation ("a hallmark of aging"), in both total peritoneal leukocyte population and isolated peritoneal macrophages...
March 9, 2017: Redox Biology
https://www.readbyqxmd.com/read/28319775/dilute-alkali-pretreatment-of-softwood-pine-a-biorefinery-approach
#14
Ali Safari, Keikhosro Karimi, Marzieh Shafiei
Dilute alkali pretreatment was performed on softwood pine to maximize ethanol and biogas production via a biorefinery approach. Alkali pretreatments were performed with 0-2% w/v NaOH at 100-180°C for 1-5h. The liquid fraction of the pretreated substrates was subjected to anaerobic digestion. The solid fraction of the pretreatment was used for separate enzymatic hydrolysis and fermentation. High ethanol yields of 76.9‒78.0% were achieved by pretreatment with 2% (w/v) NaOH at 180°C. The highest biogas yield of 244mL/g volatile solid (at 25°C, 1bar) was achieved by the pretreatment with 1% (w/v) NaOH at 180°C...
March 7, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28319770/mechanisms-and-effects-of-arsanilic-acid-on-antibiotic-resistance-genes-and-microbial-communities-during-pig-manure-digestion
#15
Wei Sun, Xun Qian, Jie Gu, Xiao-Juan Wang, Li Zhang, Ai-Yun Guo
High concentrations of residual arsanilic acid occur in pig manure due to its use in feed to promote growth and control diseases. This study compared the effects of arsanilic acid at three concentrations (0, 325, and 650mg/kg dry pig manure) on the abundance of antibiotic resistance genes (ARGs) and the microbial community during anaerobic digestion. Addition of 650mg/kg arsanilic acid enhanced the absolute abundances of tetC, sul2, ermB, and gyrA more than twofold in the digestion product. Redundancy analysis indicated that the change in the microbial community structure was the main driver of variation in the ARGs profile...
March 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28319766/sequential-ultrasound-and-low-temperature-thermal-pretreatment-process-optimization-and-influence-on-sewage-sludge-solubilization-enzyme-activity-and-anaerobic-digestion
#16
Patricio Neumann, Zenón González, Gladys Vidal
The influence of sequential ultrasound and low-temperature (55°C) thermal pretreatment on sewage sludge solubilization, enzyme activity and anaerobic digestion was assessed. The pretreatment led to significant increases of 427-1030% and 230-674% in the soluble concentrations of carbohydrates and proteins, respectively, and 1.6-4.3 times higher enzymatic activities in the soluble phase of the sludge. Optimal conditions for chemical oxygen demand solubilization were determined at 59.3kg/L total solids (TS) concentration, 30,500kJ/kg TS specific energy and 13h thermal treatment time using response surface methodology...
March 9, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28319707/treatment-of-anaerobic-digested-effluent-in-biochar-packed-vertical-flow-constructed-wetland-columns-role-of-media-and-tidal-operation
#17
Simon Kizito, Tao Lv, Shubiao Wu, Zeeshan Ajmal, Hongzhen Luo, Renjie Dong
Three types of vertical flow constructed wetland columns (VFCWs), packed with corn cob biochar (CB-CW), wood biochar (WB-CW) and gravel (G-CW) under tidal flow operations, were comparatively evaluated to investigate anaerobic digested effluent treatment performance and mechanisms. It was demonstrated that CB-CW and WB-CW provide significantly higher removal efficiencies for organic matter (>59%), NH4(+)-N (>76%), TN (>37%) and phosphorus (>71%), compared with G-CW (22%-49%). The higher pollutants removal ability of biochar-packed VFCWs was mainly attribute to the higher adsorption ability and microbial cultivation in the porous biochar media...
March 15, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28319582/trends-in-the-purchase-of-surgical-care-in-the-community-by-the-veterans-health-administration-va
#18
Amy K Rosen, William O'Brien, Qi Chen, Michael Shwartz, Kamal F M Itani, William Gunnar
BACKGROUND: The 2014 implementation of the Veterans Choice Program increased opportunities for Veterans to receive care in the community. Although surgical care is a Veterans Health Administration (VA) priority, little is known about the types of surgeries provided in the VA versus those referred to community care (CC), and whether Veterans are increasing their use of surgical care through CC with these additional opportunities. OBJECTIVES: To examine national trends across VA facilities in the frequencies and types of surgeries provided in the VA and through CC, and explore the association between facilities' purchase of care with rurality and surgical complexity designation...
March 17, 2017: Medical Care
https://www.readbyqxmd.com/read/28319020/in%C3%A2-vitro-preparation-and-assessment-of-radical-reducing-peptide-from-octopus-aegina-using-digestive-proteases
#19
Sekar Sudhakar, Rasool Abdul Nazeer
Antioxidant peptides protect biological macromolecules against radical damages. The use of these peptides was evaluated using free radicals scavenging assays [2,2-diphenyl-1 picrylhydrazyl (DPPH) and hydroxyl] with the help of UV-visible and electron spin resonance (ESR) spectroscopy methods. The Octopus aegina mantle protein were tested upon hydrolysis using gastrointestinal enzymes up to 12 h, where pepsin hydrolysate exhibited superior properties (DPPH: 44.39±0.67% and hydroxyl: 38.84±1.07%) compared with trypsin and α-chymotrypsin...
March 16, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28318963/an-investigation-of-ultrasound-effect-on-digestate-solubilization-and-methane-yield
#20
Temesgen Garoma, David Pappaterra
In this study, different ultrasound power intensities of 0.05-0.21kW/L (4.13-16.52kWh/kg TS) were applied at a frequency of 20kHz and for durations of 5-20min to digestate obtained from a domestic wastewater treatment plant. The ultrasonic effect on digestate solubilization was revealed by increased levels of soluble Chemical Oxygen Demand (sCOD), soluble Total Organic Carbon (sTOC), and soluble Total Nitrogen (sTN) released into the solution. The highest material release was achieved at an ultrasonic energy intensity of 0...
March 16, 2017: Waste Management
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