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https://www.readbyqxmd.com/read/28646761/regulation-on-the-aggregation-induced-emission-aie-of-dna-templated-silver-nanoclusters-by-bsa-and-its-hydrolysates
#1
Wei-Xian Wang, Yuqing Wu, Hong-Wei Li
The aggregation-induced emission (AIE) phenomenon of the metal nanoclusters (NCs) has been discovered quite recently, which is considered to be creative to improve the optical properties of NCs upon ligand aggregation. In the present study, we report an AIE system for double-stranded DNA-templated silver nanoclusters (AgNCs-dsDNA) by using bovine serum albumin (BSA) in solution, which induce a moderate emission enhancement of AgNCs (5-fold) and a blue-shift through a loosen interaction. In addition, significant luminescence enhancement (30-fold) is further stimulated by the addition of digestive enzyme to the system by altering the surface structure of the aggregated particles...
June 10, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28646659/analysis-of-recombinant-monoclonal-antibodies-in-hydrophilic-interaction-chromatography-a-generic-method-development-approach
#2
Balázs Bobály, Valentina D'Atri, Alain Beck, Davy Guillarme, Szabolcs Fekete
Hydrophilic interaction liquid chromatography (HILIC) is a well-established technique for the separation and analysis of small polar compounds. A recently introduced widepore stationary phase expanded HILIC applications to larger molecules, such as therapeutic proteins. In this paper, we present some generic HILIC conditions adapted for a wide range of FDA and EMA approved recombinant monoclonal antibody (mAb) species and for an antibody-drug conjugate (ADC). Seven approved mAbs possessing various isoelectric point (pI) and hydrophobicity as well as a cysteine conjugated ADC were used in this study...
June 15, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28646608/fluid-filling-of-the-digestive-tract-for-improved-proton-resonance-frequency-shift-based-mr-thermometry-in-the-pancreas
#3
Cyril J Ferrer, Lambertus W Bartels, Marijn van Stralen, Baudouin Denis de Senneville, Chrit T W Moonen, Clemens Bos
PURPOSE: To demonstrate that fluid filling of the digestive tract improves the performance of respiratory motion-compensated proton resonance frequency shift (PRFS)-based magnetic resonance (MR) thermometry in the pancreas. MATERIALS AND METHODS: In seven volunteers (without heating), we evaluated PRFS thermometry in the pancreas with and without filling of the surrounding digestive tract. All data acquisition was performed at 1.5T, then all datasets were analyzed and compared with three different PRFS respiratory motion-compensated thermometry methods: gating, multibaseline, and referenceless...
June 24, 2017: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/28646111/a-bacillus-paralicheniformis-iron-containing-urease-reduces-urea-concentrations-in-rice-wine
#4
Qingtao Liu, Yuqi Chen, Minglai Yuan, Guocheng Du, Jian Chen, Zhen Kang
Urease, as a nickel-containing metalloenzyme, was the first enzyme to be crystallized, and it has a prominent position in the history of biochemistry. In the present study, we identified a nickel urease gene cluster, ureABCEFGDH, from Bacillus paralicheniformis ATCC 9945a and characterized it in Escherichia coli Enzymatic assays demonstrate that this oxygen-stable urease is also an iron-containing acid urease. UreH heterologous expression assays suggest that this accessory protein is involved in transmembrane transportation of nickel and iron ions...
June 23, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28646033/implementing-a-reference-measurement-system-for-c-peptide-successes-and-lessons-learned
#5
Randie R Little, Robert I Wielgosz, Ralf Josephs, Tomoya Kinumi, Akiko Takatsu, Hongmei Li, Daniel Stein, Chris Burns
BACKGROUND: Assessment of endogenous insulin secretion by measuring C-peptide concentrations is widely accepted. Recent studies have shown that preservation of even small amounts of endogenous C-peptide production in patients with type 1 diabetes reduces risks for diabetic complications. Harmonization of C-peptide results will facilitate comparison of data from different research studies and later among clinical laboratory results at different sites using different assay methods. CONTENT: This review provides an overview of the general process of harmonization and standardization and the challenges encountered with implementing a reference measurement system for C-peptide...
June 23, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28645702/use-of-adipose-tissue-and-stromal-vascular-fraction-in-hand-surgery
#6
REVIEW
Iad Nseir, Flore Delaunay, Charles Latrobe, Albane Bonmarchand, Dorothée Coquerel-Beghin, Isabelle Auquit-Auckbur
Adipose tissue is an abundant source of various cell types including not only adipocytes, but also progenitor and endothelial cells from thestroma. Interest in adipose tissue has surged since the identification in 2001 of adipose-derived stem cells (ADSCs) and of the stromal vascular fraction (SVF) obtained from adipose tissue by enzymatic digestion and centrifugation. SVF has been proven effective in ensuring tissue regeneration, thus improving tissue trophicityand vascularisation. These effects have generated strong interest among both physicians and surgeons, particularly in the field of hand surgery...
June 20, 2017: Orthopaedics & Traumatology, Surgery & Research: OTSR
https://www.readbyqxmd.com/read/28645424/occurrence-of-selected-trace-metals-and-their-oral-bioaccessibility-in-urban-soils-of-kindergartens-and-parks-in-bratislava-slovak-republic-as-evaluated-by-simple-in-vitro-digestion-procedure
#7
Edgar Hiller, Martin Mihaljevič, Lenka Filová, Lucia Lachká, Ľubomír Jurkovič, Tatsiana Kulikova, Katarína Fajčíková, Mária Šimurková, Veronika Tatarková
A total of eighty surface soil samples were collected from public kindergartens and urban parks in the city of Bratislava, and the <150µm soil fraction was evaluated for total concentrations of five metals, Cd, Cu, Hg, Pb and Zn, their oral bioaccessibilities, non-carcinogenic and carcinogenic health risks to children, and lead isotopic composition. The mean metal concentrations in urban soils (0.29, 36.1, 0.13, 30.9 and 113mg/kg for Cd, Cu, Hg, Pb and Zn, respectively) were about two times higher compared with background soil concentrations...
June 20, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28645369/sequencing-dna-for-the-oxidatively-modified-base-8-oxo-7-8-dihydroguanine
#8
Aaron M Fleming, Yun Ding, Cynthia J Burrows
The DNA base guanine (G) can be oxidatively modified to 8-oxo-7,8-dihydroguanine (OG). Extraction of genomic DNA followed by nuclease digestion and mass spectrometry analysis has found OG is present at background levels of ~1 out of 10(6) Gs; however, this approach cannot determine the locations for the OGs in the genome. Thus, in this methods report, we outline three different methods (A, B, and C) for sequencing OG in DNA. Method A sequences OG by utilizing the base excision repair pathway to delete the OG nucleotide from the DNA that is then detected by Sanger sequencing as a deletion signature...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28645315/role-of-inhibitors-of-serine-peptidases-in-protecting-leishmania-donovani-against-the-hydrolytic-peptidases-of-sand-fly-midgut
#9
Sudha Verma, Sushmita Das, Abhishek Mandal, Md Yousuf Ansari, Sujata Kumari, Rani Mansuri, Ajay Kumar, Ruby Singh, Savita Saini, Kumar Abhishek, Vijay Kumar, Ganesh Chandra Sahoo, Pradeep Das
BACKGROUND: In vector-borne diseases such as leishmaniasis, the sand fly midgut is considered to be an important site for vector-parasite interaction. Digestive enzymes including serine peptidases such as trypsin and chymotrypsin, which are secreted in the midgut are one of the obstacles for Leishmania in establishing a successful infection. The presence of some natural inhibitors of serine peptidases (ISPs) has recently been reported in Leishmania. In the present study, we deciphered the role of these ISPs in the survival of Leishmania donovani in the hostile sand fly midgut environment...
June 23, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28644862/high-intensity-corneal-collagen-crosslinking-with-riboflavin-and-uva-in-rat-cornea
#10
Yirui Zhu, Peter S Reinach, Hanlei Zhu, Qiufan Tan, Qinxiang Zheng, Jia Qu, Wei Chen
Corneal collagen cross-linking (CXL) halts human corneal ectasias progression by increasing stromal mechanical stiffness. Although some reports describe that this procedure is effective in dealing with some infectious and immunologic corneal thinning diseases, there is a need for more animal models whose corneal thickness more closely resemble those occurring in these patients. To meet this need, we describe here high-intensity protocols that are safe and effective for obtaining CXL in rat corneas. Initially, a range of potentially effective UVA doses were evaluated based on their effectiveness in increasing tissue enzymatic resistance to dissolution...
2017: PloS One
https://www.readbyqxmd.com/read/28644752/expression-and-purification-of-swine-rag2-in-e-coli-for-production-of-porcine-rag2-polyclonal-antibodies
#11
Yu-Bei Jin, Wen-Tao Yang, Ke-Yan Huang, Hong-Liang Chen, Seria-Masole Shonyela, Jing Liu, Qiong Liu, Bo Feng, You Zhou, Shu-Li Zhi, Yan-Long Jiang, Jian-Zhong Wang, Hai-Bin Huang, Chun-Wei Shi, Gui-Lian Yang, Chun-Feng Wang
Recombination activating gene 2 (RAG2) is necessary for immature B cell differentiation. Antibodies to human and rabbit RAG2 are currently commercially available, but antibodies to swine RAG remain unavailable to date. In this study, the swine RAG2 genes sequence was synthesized and then cloned into a pET-28a vector. The recombinant fusion protein was successfully expressed in E. coli, purified through nickel column chromatography, and further digested with Tobacco Etch Virus protease. The cleaved protein was purified by molecular-exclusion chromatography and named pRAG2...
June 23, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28644442/linking-the-development-and-functioning-of-a-carnivorous-pitcher-plant-s-microbial-digestive-community
#12
David W Armitage
Ecosystem development theory predicts that successional turnover in community composition can influence ecosystem functioning. However, tests of this theory in natural systems are made difficult by a lack of replicable and tractable model systems. Using the microbial digestive associates of a carnivorous pitcher plant, I tested hypotheses linking host age-driven microbial community development to host functioning. Monitoring the yearlong development of independent microbial digestive communities in two pitcher plant populations revealed a number of trends in community succession matching theoretical predictions...
June 23, 2017: ISME Journal
https://www.readbyqxmd.com/read/28644081/effects-of-added-lipids-on-digestibility-and-nitrogen-balance-in-oiled-common-murres-uria-aalge-and-western-grebes-aechmophorus-occidentalis-fed-four-formulations-of-a-critical-care-diet
#13
Rebecca S Duerr, Kirk C Klasing
Nutritional support is a primary therapy administered to oiled animals during responses to oil spills, but data informing nutritional decision-making during events are limited. In this study, 44 common murres ( Uria aalge ) and 6 Western grebes ( Aechmophorus occidentalis ), naturally oiled by oceanic seeps off the coast of Ventura and Santa Barbara Counties, CA, USA, were assigned to 1 of 4 groups fed diets with varying levels (6.8% [no added oil], 11%, and 20%) and types (salmon, corn) of oil added to a partially purified basal diet...
June 2017: Journal of Avian Medicine and Surgery
https://www.readbyqxmd.com/read/28643898/identification-of-cross-reactive-b-cell-epitopes-between-bos-d-9-0101-bos-taurus-and-gly-m-5-0101-glycine-max-by-epitope-mapping-maldi-tof-ms
#14
Ángela María Candreva, Mario Ferrer-Navarro, Silvia Bronsoms, Alejandra Quiroga, Renata Curciarello, Ana Cauerhff, Silvana Petruccelli, Guillermo Horacio Docena, Sebastián Alejandro Trejo
Exposure to cow's milk constitutes one of the most common causes of food allergy. In addition, exposure to soy proteins has become relevant in a restricted proportion of milk allergic pediatric patients treated with soy formulae as a dairy substitute, because of the cross-allergenicity described between soy and milk proteins . We have previously identified several cross-reactive allergens between milk and soy that may explain this intolerance. The purpose of the present work was to identify epitopes in the purified αS1-casein and the recombinant soy allergen Gly m 5...
June 23, 2017: Proteomics
https://www.readbyqxmd.com/read/28643668/how-to-perform-a-suture-with-a-5-mm-trocar-by-laparoscopy
#15
E Nohuz, M Albaut, L De Simone, G Chêne
During laparoscopy using only 5mm trocars, it may sometimes be necessary to perform a suture, which usually requires the use of a 10mm or 12mm trocar with a reducer. Thus, the 5-mm trocar has to be replaced by a larger diameter device. In order to avoid the trocar change in these situations, a trick that is easily realizable for performing one or more stitches is described. This technique can be used in various surgical specialties, whether it is gynecological, digestive or urological surgery.
April 2017: Journal of gynecology obstetrics and human reproduction
https://www.readbyqxmd.com/read/28642676/international-society-of-sports-nutrition-position-stand-protein-and-exercise
#16
REVIEW
Ralf Jäger, Chad M Kerksick, Bill I Campbell, Paul J Cribb, Shawn D Wells, Tim M Skwiat, Martin Purpura, Tim N Ziegenfuss, Arny A Ferrando, Shawn M Arent, Abbie E Smith-Ryan, Jeffrey R Stout, Paul J Arciero, Michael J Ormsbee, Lem W Taylor, Colin D Wilborn, Doug S Kalman, Richard B Kreider, Darryn S Willoughby, Jay R Hoffman, Jamie L Krzykowski, Jose Antonio
The International Society of Sports Nutrition (ISSN) provides an objective and critical review related to the intake of protein for healthy, exercising individuals. Based on the current available literature, the position of the Society is as follows:An acute exercise stimulus, particularly resistance exercise, and protein ingestion both stimulate muscle protein synthesis (MPS) and are synergistic when protein consumption occurs before or after resistance exercise.For building muscle mass and for maintaining muscle mass through a positive muscle protein balance, an overall daily protein intake in the range of 1...
2017: Journal of the International Society of Sports Nutrition
https://www.readbyqxmd.com/read/28642078/experimental-tests-on-commercial-sweet-product-residue-spr-as-a-suitable-feed-for-anaerobic-bioenergy-h2-ch4-production
#17
Andrea C Luongo Malavè, Debora Fino, Carlos E Gómez Camacho, Bernardo Ruggeri
Food stores can find themselves in the position of having to dispose of different types of products, such as snacks, confectionery, prepackaged food, drinks and others, Sweet Product Residue (SPR), which presents a great opportunity to produce energy through Anaerobic Digestion (AD), due to its high sugar, carbohidrate and fat contents. In order to valorise SPR, this paper takes into consideration the all necessary treatments; owing the fact that the refuses are constitute by an organic part and packaging (plastic, paper and their combinations), a pretreatment able to remove the latter is necessary...
June 19, 2017: Waste Management
https://www.readbyqxmd.com/read/28642075/greenhouse-gas-emissions-from-different-municipal-solid-waste-management-scenarios-in-china-based-on-carbon-and-energy-flow-analysis
#18
Yili Liu, Weixin Sun, Jianguo Liu
Waste management is a major source of global greenhouse gas (GHG) emissions and many opportunities exist to reduce these emissions. To identify the GHG emissions from waste management in China, the characteristics of MSW and the current and future treatment management strategies, five typical management scenarios were modeled by EaseTech software following the principles of life cycle inventory and analyzed based on the carbon and energy flows. Due to the high organic fraction (50-70%) and moisture content (>50%) of Chinese municipal solid waste (MSW), the net GHG emissions in waste management had a significant difference from the developed countries...
June 19, 2017: Waste Management
https://www.readbyqxmd.com/read/28642062/lrh-1-senses-signaling-from-phosphatidylcholine-to-regulate-the-expansion-growth-of-digestive-organs-via-synergy-with-wnt-%C3%AE-catenin-signaling-in-zebrafish
#19
Gang Zhai, Jia Song, Tingting Shu, Junjun Yan, Xia Jin, Jiangyan He, Zhan Yin
Liver receptor homolog-1 (LRH-1) is an orphan nuclear receptor that is critical for the growth and proliferation of cancer cells and other biological processes, including lipid transportation and metabolism, sexual determination and steroidogenesis. However, because homozygous lrh-1(-/-) mice die in utero, the regulatory mechanisms involved in embryonic development mediated by this receptor are poorly understood. In the present study, we performed transcription activator-like effector nuclease (TALEN)-mediated loss-of-function assays, taking advantage of zebrafish external fertilization, to investigate the function of lrh-1...
April 27, 2017: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/28642052/impact-of-planetary-ball-mills-on-corn-stover-characteristics-and-enzymatic-digestibility-depending-on-grinding-ball-properties
#20
Jin Hyung Lee, Jeong Heo Kwon, Tae Hyun Kim, Won Il Choi
The impact of planetary mill pretreatment on corn stover size, crystallinity, and enzyme digestibility was investigated based on the grinding ball properties in an effort to reduce energy consumption. Alumina, zirconia, and steel balls were used, of which steel balls had the highest specific gravity but the lowest surface roughness while alumina balls had the lowest specific gravity and the highest roughness. The planetary mill pretreatment effectively reduced the size and crystallinity of the corn stover for all the ball types...
June 12, 2017: Bioresource Technology
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