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https://www.readbyqxmd.com/read/29149777/investigating-the-association-of-adam33-single-nucleotide-polymorphisms-snps-with-susceptibility-to-allergic-asthma-in-azerbaijan-population-of-iran-a-case-control-study
#1
Ilghar Zeinaly, Mahnaz Sadeghi-Shabestrai, Zohreh Babaloo, Alireza Razavi, Mohammad Sajay-Asbaghi, Saeed Sadigh-Eteghad, Tohid Kazemi
Asthma, affecting a growing number of populations, is a clinical condition with complex cellular and genetic factors. Single nucleotide polymorphisms (SNPs) in gene coding for molecules, which play major roles in the immunopathogenesis of asthma have been considered recently as genetic predisposing factors this disease. Possible association between two SNPs in a disintegrin and metalloprotease 33 (ADAM33), which participates in airway remodeling, and susceptibility to asthma was studied in this study. 190 patients with asthma and 180 healthy controls were enrolled in this case-control study...
October 2017: Iranian Journal of Allergy, Asthma, and Immunology
https://www.readbyqxmd.com/read/29149247/quantitative-proteomic-profiling-of-bovine-follicular-fluid-during-follicle-development
#2
Rodrigo de Andrade Ferrazza, Elizabeth Moreira Dos Santos Schmidt, Monika Mihm Carmichael, Fabiana Ferreira de Souza, Richard Burchmore, Roberto Sartori, Peter David Eckersall, João Carlos Pinheiro Ferreira
Bovine follicular fluid (FF) constitutes the microenvironment of follicles and includes various biologically active proteins. We performed a study involving 18 healthy non-lactating Holstein cows to determine the protein expression profile of FF at key-stages of follicular development. Follicles were individually aspirated in vivo at pre-deviation (F1∼7.0mm); deviation (F1∼8.5mm); post-deviation (F1∼12.0mm); and pre-ovulatory stages of follicle development, which were confirmed by measurement of follicular estradiol and progesterone concentrations...
November 15, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29149222/personalized-medicine-and-hispanic-health-improving-health-outcomes-and-reducing-health-disparities-a-national-heart-lung-and-blood-institute-workshop-report
#3
M Larissa Avilés-Santa, John Heintzman, Nangel M Lindberg, Rafael Guerrero-Preston, Kenneth Ramos, Ana L Abraído-Lanza, Jonca Bull, Adolph Falcón, Mary Ann McBurnie, Ernest Moy, George Papanicolaou, Ileana L Piña, Jennifer Popovic, Shakira F Suglia, Miguel A Vázquez
Persons of Hispanic/Latino descent may represent different ancestries, ethnic and cultural groups and countries of birth. In the U.S., the Hispanic/Latino population is projected to constitute 29% of the population by 2060. A personalized approach focusing on individual variability in genetics, environment, lifestyle and socioeconomic determinants of health may advance the understanding of some of the major factors contributing to the health disparities experienced by Hispanics/Latinos and other groups in the U...
2017: BMC Proceedings
https://www.readbyqxmd.com/read/29149196/easy-xeno-free-and-feeder-free-method-for-isolating-and-growing-limbal-stromal-and-epithelial-stem-cells-of-the-human-cornea
#4
Djida Ghoubay-Benallaoua, Céline de Sousa, Raphaël Martos, Gaël Latour, Marie-Claire Schanne-Klein, Elisabeth Dupin, Vincent Borderie
Epithelial and stromal stem cells are required to maintain corneal transparency. The aim of the study was to develop a new method to isolate and grow both corneal stromal (SSC) and epithelial limbal (LSC) stem cells from small human limbal biopsies under culture conditions in accordance with safety requirements mandatory for clinical use in humans. Superficial limbal explants were retrieved from human donor corneo-scleral rims. Human limbal cells were dissociated by digestion with collagenase A, either after epithelial scraping or with no scraping...
2017: PloS One
https://www.readbyqxmd.com/read/29149068/a-new-uplc-ms-ms-method-for-the-characterization-and-discrimination-of-polysaccharides-from-genus-ephedra-based-on-enzymatic-digestions
#5
Yong-Gang Xia, Tian-Long Wang, Li-Ming Sun, Jun Liang, Bing-You Yang, Hai-Xue Kuang
Ephedra sinica polysaccharides have been reported to possess important activities, so quality evaluation of polysaccharides from the genus Ephedra is urgent. In this study, enzymatic digestions were performed to establish multiple saccharide fingerprints by ultra-performance liquid chromatography with electrospray ionization triple quadrupole linear ion trap mass spectrometry (UPLC-ESI-TQ-MS/MS) based on a multiple-reaction monitoring in negative mode. Under optimum UPLC-ESI⁻-TQ-MS/MS conditions, excellent separation and quantification of 21 constituents were achieved within 20 min on a solid core column with a 1...
November 17, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29148971/convergence-of-topological-domain-boundaries-insulators-and-polytene-interbands-revealed-by-high-resolution-mapping-of-chromatin-contacts-in-the-early-drosophila-melanogaster-embryo
#6
Michael R Stadler, Jenna E Haines, Michael Eisen
High-throughput assays of three-dimensional interactions of chromosomes have shed considerable light on the structure of animal chromatin. Despite this progress, the precise physical nature of observed structures and the forces that govern their establishment remain poorly understood. Here we present high resolution Hi-C data from early Drosophila embryos. We demonstrate that boundaries between topological domains of various sizes map to DNA elements that resemble classical insulator elements: short genomic regions sensitive to DNase digestion that are strongly bound by known insulator proteins and are frequently located between divergent promoters...
November 17, 2017: ELife
https://www.readbyqxmd.com/read/29148801/neuropeptidomics-of-the-bed-bug-cimex-lectularius
#7
Reinhard Predel, Susanne Neupert, Christian Derst, Klaus Reinhardt, Christian Wegener
The bed bug Cimex lectularius is a globally distributed human ectoparasite with fascinating biology. It has recently acquired resistance against a broad range of insecticides, causing a worldwide increase in bed bug infestations. The recent annotation of the bed bug genome revealed a full complement of neuropeptide and neuropeptide receptor genes in this species. With regard to the biology of C. lectularius, neuropeptide signalling is especially interesting since it regulates feeding, diuresis, digestion as well as reproduction, and also provides potential new targets for chemical control...
November 17, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29148730/the-effect-of-different-treatment-technologies-on-the-fate-of-antibiotic-resistance-genes-and-class-1-integrons-when-residual-municipal-wastewater-solids-are-applied-to-soil
#8
Tucker Burch, Michael J Sadowsky, Timothy M LaPara
Residual wastewater solids are a significant reservoir of antibiotic resistance genes (ARGs). While treatment technologies can reduce ARG levels in residual wastewater solids, the effects of these technologies on ARGs in soil during subsequent land-application are unknown. In this study we investigated the use of numerous treatment technologies (air drying, aerobic digestion, mesophilic anaerobic digestion, thermophilic anaerobic digestion, pasteurization, and alkaline stabilization) on the fate of ARGs and class 1 integrons in wastewater solids-amended soil microcosms...
November 17, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/29147970/effect-of-different-salinities-on-gene-expression-and-activity-of-digestive-enzymes-in-the-thick-lipped-grey-mullet-chelon-labrosus
#9
REVIEW
I M Pujante, F J Moyano, J A Martos-Sitcha, J M Mancera, G Martínez-Rodríguez
The effects of different environmental salinities (0, 12, 40, and 55 ppt) on pepsinogen 2 (pga2), trypsinogen 2 (try2), chymotrypsinogen (ctr), and pancreatic alpha-amylase (amy2a) gene expression, and on the total activities of their corresponding enzymes, were assessed in Chelon labrosus juveniles, after their corresponding full-complementary DNA sequences were cloned. Furthermore, the quantitative effect of different salinities on the hydrolysis of feed protein by fish digestive enzymes was evaluated using an in vitro system...
November 17, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29147852/enhanced-peptide-detection-toward-single-neuron-proteomics-by-reversed-phase-fractionation-capillary-electrophoresis-mass-spectrometry
#10
Sam B Choi, Camille Lombard-Banek, Pablo Muñoz-LLancao, M Chiara Manzini, Peter Nemes
The ability to detect peptides and proteins in single cells is vital for understanding cell heterogeneity in the nervous system. Capillary electrophoresis (CE) nanoelectrospray ionization (nanoESI) provides high-resolution mass spectrometry (HRMS) with trace-level sensitivity, but compressed separation during CE challenges protein identification by tandem HRMS with limited MS/MS duty cycle. Here, we supplemented ultrasensitive CE-nanoESI-HRMS with reversed-phase (RP) fractionation to enhance identifications from protein digest amounts that approximate to a few mammalian neurons...
November 16, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29147091/evaluation-of-the-digestibility-of-korean-hanwoo-beef-cuts-using-the-in-vitro-physicochemical-upper-gastrointestinal-system
#11
Ji-Hye Jeon, Michelle Yoo, Tae-Hwan Jung, Woo-Min Jeon, Kyoung-Sik Han
The aim of this study was to investigate the digestibility of different Korean Hanwoo beef cuts using an in vitro digestion model, in vitro physicochemical upper gastrointestinal system (IPUGS). The four most commonly consumed cuts - tenderloin, sirloin, brisket and flank, and bottom round - were chosen for this study. Beef samples (75 g) were cooked and ingested into IPUGS, which was composed of mouth, esophagus, and stomach, thereby simulating the digestion conditions of humans. Digested samples were collected every 15 min for 4 h of simulation and their pH monitored...
2017: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/29147019/identification-and-characterization-of-serine-protease-inhibitors-in-a-parasitic-wasp-pteromalus-puparum
#12
Lei Yang, Yaotian Mei, Qi Fang, Jiale Wang, Zhichao Yan, Qisheng Song, Zhe Lin, Gongyin Ye
Serine protease inhibitors (SPIs) regulate protease-mediated activities by inactivating their cognate proteinases, and are involved in multiple physiological processes. SPIs have been extensively studied in vertebrates and invertebrates; however, little SPI information is available in parasitoids. Herein, we identified 57 SPI genes in total through the genome of a parasitoid wasp, Pteromalus puparum. Gene structure analyses revealed that these SPIs contain 7 SPI domains. Depending on their mode of action, these SPIs can be categorized into serpins, canonical inhibitors and alpha-2-macroglobulins (A2Ms)...
November 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29146964/effects-of-waste-sources-on-performance-of-anaerobic-co-digestion-of-complex-organic-wastes-taking-food-waste-as-an-example
#13
Xingang Lu, Wengang Jin, Shengrong Xue, Xiaojiao Wang
Few studies have addressed how to blend wastes for anaerobic co-digestion. This study investigated the effects of waste sources on anaerobic co-digestion (AcoD) performance, by varying the quality of food wastes (FWs) from six sources in Xi'an region, China that were individually co-digested with pre-treated corn straw and cattle manure. These effects were analysed in terms of their volatile solid (VS) ratios, C/N ratios, and the chemical composition of the FWs. The results indicated that the VS ratios were not suitable as a common mixture method because the VS ratios at which the best methane potentials occurred differed significantly among the six FW groups...
November 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29146584/microrna-tissue-atlas-of-the-malaria-mosquito-anopheles-gambiae
#14
Lena Lampe, Elena A Levashina
Anopheles gambiae mosquitoes transmit the human malaria parasite Plasmodium falciparum, which causes the majority of fatal malaria cases worldwide. The hematophagous life style defines the mosquito reproductive biology and is exploited by P. falciparum for its own sexual reproduction and transmission. The two main phases of the mosquito reproductive cycle, pre-vitellogenic (PV) and post-blood meal (PBM) shape its capacity to transmit malaria. Transition between these phases is tightly coordinated to ensure homeostasis between mosquito tissues and successful reproduction...
November 16, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/29146501/antibacterial-activity-and-probiotic-characterization-of-autochthonous-paenibacillus-polymyxa-isolated-from-anabas-testudineus-bloch-1792
#15
Sebastian J Midhun, Sahadevan Neethu, A Vysakh, Damodaran Arun, E K Radhakrishnan, Mathew Jyothis
Microbial fish pathogens are prevalent in aquaculture. Control of Bacterial fish pathogens is important and bio control of pathogenic bacteria is a novel field of study. The aim of this study was to evaluate the antagonistic activity of bacteria isolated from Anabas testudineus against potent fish pathogens. The cellular components/preparations and filtered cell free culture supernatants were effective against six fish pathogens. Altogether 110 strains were isolated from fish proximal and distal intestine, out of which 10 strains were selected through well diffusion method...
November 13, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29146347/effect-of-tea-products-on-the-in-vitro-enzymatic-digestibility-of-starch
#16
Haihua Zhang, Yulan Jiang, Junxian Pan, Yangjun Lv, Jun Liu, Shikang Zhang, Yuejin Zhu
The importance of postprandial hyperglycemia in the treatment of diabetes has been recognized recently. Tea products, such as tea polyphenols (TP), epigallocatechin gallate (EGCG), matcha, and instant tea, were chosen as constituents of tea-flour food, aimed at regulating the release of glucose from starchy foods in the postprandial period. Six starches were chosen for internal composition analysis and hydrolysis studies in vitro. Corn starch, wheat starch, and lily root flour appeared to have higher resistant starch content, slower digestion profiles, and lower kinetic constants, implying sustained release of glucose in the gastrointestinal tract...
March 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29146341/oxidation-and-nitrosation-of-meat-proteins-under-gastro-intestinal-conditions-consequences-in-terms-of-nutritional-and-health-values-of-meat
#17
Diane de La Pomélie, Véronique Santé-Lhoutellier, Thierry Sayd, Philippe Gatellier
The chemical changes (oxidation/nitrosation) of meat proteins during digestion lead to a decrease in their nutritional value. Moreover, oxidized and nitrosated amino acids are suspected to promote various human pathologies. To investigate the mechanisms and the kinetics of these endogenous protein modifications, we used a dynamic artificial digestive system (DIDGI®) that mimics the physicochemical conditions of digestion. The combined effect of meat cooking and endogenous addition of ascorbate and nitrite was evaluated on protein oxidation (by measuring carbonyl groups), protein nitrosation (by measuring nitrosamines), and proteolysis...
March 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29146324/antioxidative-potentially-anti-inflammatory-and-antidiabetic-properties-as-well-as-oxidative-stability-and-acceptability-of-cakes-supplemented-with-elicited-basil
#18
Urszula Złotek
The aim of the study was to investigate the effect of addition of basil elicited with jasmonic acid (JA) on the biological properties, oxidative stability, and sensory quality of cakes. Simulated gastrointestinal digestion was used for determination of bioavailability. The antioxidant, anti-inflammatory and antidiabetic potential of fortified cakes were significantly higher than those of the control cakes. The antioxidant activity of the tested cakes was increased after addition of basil, proportionally to the amount of the additive...
March 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29146311/microbial-communities-involved-in-biogas-production-exhibit-high-resilience-to-heat-shocks
#19
Christian Abendroth, Sarah Hahnke, Claudia Simeonov, Michael Klocke, Sonia Casani-Miravalls, Patrice Ramm, Christoph Bürger, Olaf Luschnig, Manuel Porcar
We report here the impact of heat-shock treatments (55 and 70 °C) on the biogas production within the acidification stage of a two-stage reactor system for anaerobic digestion and biomethanation of grass. The microbiome proved both taxonomically and functionally very robust, since heat shocks caused minor community shifts compared to the controls, and biogas yield was not decreased. The strongest impact on the microbial profile was observed with a combination of heat shock and low pH. Since no transient reduction of microbial diversity occured after the shock, biogas keyplayers, but also potential pathogens, survived the treatment...
October 31, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29146098/characterization-of-polymorphism-in-the-fsh-receptor-gene-and-its-impact-on-some-reproductive-indices-in-dairy-cows
#20
Hassan Sharifiyazdi, Abdolah Mirzaei, Zahra Ghanaatian
Follicle stimulating hormone (FSH) is released from the anterior pituitary gland and has an important role in female fertility. As FSH is a glycoprotein polypeptide hormone which cannot pass through the cell membrane, its influence on target cells must be mediated by the FSH receptor (FSHR). Accordingly, any kind of mutation in FSHR can affect reproduction in dairy cows. In this study, the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used for recognition of a point mutation (A/G: position -278) located in the FSHR gene in Iranian dairy cows...
November 6, 2017: Animal Reproduction Science
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