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liver lncRNA

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https://www.readbyqxmd.com/read/28814771/rna-sequencing-of-db-db-mice-liver-identifies-lncrna-h19-as-a-key-regulator-of-gluconeogenesis-and-hepatic-glucose-output
#1
Neha Goyal, Ambily Sivadas, K V Shamsudheen, Rijith Jayarajan, Ankit Verma, Sridhar Sivasubbu, Vinod Scaria, Malabika Datta
Liver plays a key role in maintaining glucose homeostasis and impaired hepatic glucose metabolism is associated with type 2 diabetes. In the present study, we used RNA sequencing to profile the transcriptome of the livers of diabetic db/db mice as compared to the normal db/+ mice and identified 218 differentially expressed genes. Amongst these, there were 3 lncRNAs that were significantly downregulated and H19 was the most altered lncRNA in the livers of db/db mice. H19 expression significantly correlated with the expression of genes of the glycolysis and gluconeogenesis pathways, which suggest that altered hepatic H19 levels can directly or indirectly modulate their expression...
August 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28810927/stat3-mediated-upregulation-of-lncrna-hoxd-as1-as-a-cerna-facilitates-liver-cancer-metastasis-by-regulating-sox4
#2
Hui Wang, Xisong Huo, Xin-Rong Yang, Jia He, Lijun Cheng, Na Wang, Xuan Deng, Haojie Jin, Ning Wang, Cun Wang, Fangyu Zhao, Jingyuan Fang, Ming Yao, Jia Fan, Wenxin Qin
BACKGROUND: Several of the thousands of human long noncoding RNAs (lncRNAs) have been functionally characterized, yet their potential involvement in hepatocellular carcinoma (HCC) remains poorly understood. METHODS: LncRNA-HOXD-AS1 was identified by microarray and validated by real-time PCR. The clinicopathological significance of HOXD-AS1 was analyzed by Kaplan-Meier method. Chromatin immunoprecipitation was conducted to examine the mechanism of HOXD-AS1 upregulation...
August 14, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28803234/rna-sequencing-and-bioinformatics-analysis-implicate-the-regulatory-role-of-a-long-noncoding-rna-mrna-network-in-hepatic-stellate-cell-activation
#3
Can-Jie Guo, Xiao Xiao, Li Sheng, Lili Chen, Wei Zhong, Hai Li, Jing Hua, Xiong Ma
BACKGROUND/AIMS: To analyze the long noncoding (lncRNA)-mRNA expression network and potential roles in rat hepatic stellate cells (HSCs) during activation. METHODS: LncRNA expression was analyzed in quiescent and culture-activated HSCs by RNA sequencing, and differentially expressed lncRNAs verified by quantitative reverse transcription polymerase chain reaction (qRT-PCR) were subjected to bioinformatics analysis. In vivo analyses of differential lncRNA-mRNA expression were performed on a rat model of liver fibrosis...
August 11, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28771824/down-regulation-of-lncrna-neat1-alleviated-the-non-alcoholic-fatty-liver-disease-via-mtor-s6k1-signaling-pathway
#4
Xiang Wang
BACKGROUND: Without effective medical interventions for complete reverse of NAFLD, it needs to urgently explore the underlying molecular mechanisms of non-alcoholic fatty liver disease (NAFLD) to offer a novel therapeutic strategy for people suffering from NAFLD. METHODS: Sprague-Dawley (SD) rats were used to establish the NAFLD animal model. Lipofectamine 2000 was used to silence or over-express NEAT1. The expression of NEAT1 and the mRNA levels of ACC and FAS were determined by qRT-PCR...
August 3, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28747678/the-liver-enriched-lnc-lfar1-promotes-liver-fibrosis-by-activating-tgf%C3%AE-and-notch-pathways
#5
Kun Zhang, Xiaohui Han, Zhen Zhang, Lina Zheng, Zhimei Hu, Qingbin Yao, Hongmei Cui, Guiming Shu, Maojie Si, Chan Li, Zhemin Shi, Ting Chen, Yawei Han, Yanan Chang, Zhi Yao, Tao Han, Wei Hong
Long noncoding RNAs (lncRNAs) play important roles in various biological processes such as proliferation, cell death and differentiation. Here, we show that a liver-enriched lncRNA, named liver fibrosis-associated lncRNA1 (lnc-LFAR1), promotes liver fibrosis. We demonstrate that lnc-LFAR1 silencing impairs hepatic stellate cells (HSCs) activation, reduces TGFβ-induced hepatocytes apoptosis in vitro and attenuates both CCl4- and bile duct ligation-induced liver fibrosis in mice. Lnc-LFAR1 promotes the binding of Smad2/3 to TGFβR1 and its phosphorylation in the cytoplasm...
July 26, 2017: Nature Communications
https://www.readbyqxmd.com/read/28743867/using-rna-sequencing-to-identify-putative-competing-endogenous-rnas-cernas-potentially-regulating-fat-metabolism-in-bovine-liver
#6
Ruobing Liang, Bo Han, Qian Li, Yuwei Yuan, Jianguo Li, Dongxiao Sun
RNA sequencing has been extensively used to study specific gene expression patterns to discover potential key genes related to complex traits of interest in animals. Of note, a new regulatory mechanism builds a large-scale regulatory network among transcriptome, where lncRNAs act as competing endogenous RNAs (ceRNAs) to sponge miRNAs to regulate the expression of miRNA target genes post-transcriptionally. In this study, we sequenced the cDNA and sRNA libraries of nine liver samples from three Holstein cows during dry period, early lactation, and peak of lactation with HiSeq platform...
July 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28732670/long-non-coding-rna-neat1-overexpression-is-associated-with-unfavorable-prognosis-in-patients-with-hepatocellular-carcinoma-after-hepatectomy-a-chinese-population-based-study
#7
Z Liu, Q Chang, F Yang, B Liu, H-W Yao, Z-G Bai, C-S Pu, X-M Ma, Y Yang, T-T Wang, W Guo, X-N Zhou, Z-T Zhang
AIM: To identify the association between the expression of lncRNA NEAT1 and clinicopathological characteristics of patients with HCC, and to explore the prognostic significance of lncRNA NEAT1 in predicting prognosis of HCC. METHODS: We retrospectively reviewed 86 patients with HCC (35 female, 51 male) managed in our institution between 2009 and 2014. The expression level of lncRNA NEAT1 was detected by real-time PCR. Prognostic factors were evaluated using Kaplan-Meier curves and Cox proportional hazards models...
June 28, 2017: European Journal of Surgical Oncology
https://www.readbyqxmd.com/read/28729955/identification-of-four-prognostic-lncrnas-for-survival-prediction-of-patients-with-hepatocellular-carcinoma
#8
Zhonghao Wang, Qian Wu, Shu Feng, Yanhua Zhao, Chuanmin Tao
BACKGROUND: As the fifth most common cancer worldwide, Hepatocellular carcinoma (HCC) is also the third most common cause of cancer-related death in China. Several lncRNAs have been demonstrated to be associated with occurrence and prognosis of HCC. However, identification of prognostic lncRNA signature for HCC with expression profiling data has not been conducted yet. METHODS: With the reuse of public available TCGA data, expression profiles of lncRNA for 371 patients with HCC were obtained and analyzed to find the independent prognostic lncRNA...
2017: PeerJ
https://www.readbyqxmd.com/read/28722813/knockdown-of-long-non-coding-rna-malat1-inhibits-growth-and-motility-of-human-hepatoma-cells-via-modulation-of-mir-195
#9
Dingli Liu, Yun Zhu, Jinke Pang, Xie Weng, Xiaorong Feng, Yabing Guo
The metastasis-associated lung adenocarcinoma transcription 1 (Malat1) is a long non-coding RNA (lncRNA), exerts oncogenic role in multiple cancers, including hepatocellular carcinoma (HCC). This study was aimed to investigate its posttranscriptional regulation in HCC cells. RT-PCR was performed to monitor the expression levels of Malat1 in normal liver and HCC cell lines. The expression of Malat1, microRNA (miR)-195 and epidermal growth factor receptor (EGFR) in HepG2 and MHCC97 cells was respectively or synchronously altered by transfection...
July 19, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28713968/inhibition-of-proliferation-and-invasion-of-hepatocellular-carcinoma-cells-by-lncrna-aslnc02525-silencing-and-the-mechanism
#10
Zi Chen, Dongwen Xu, Tao Zhang
One of the most differentially expressed long non-coding RNAs (lncRNAs) that we identified by high throughput screening from liver cancer and para-carcinoma tissues, ASLNC02525, was highly expressed in the tissues and cell lines of liver cancer but not in adjacent tissues or normal hepatic cells. Knockdown of ASLNC02525 in hepatocellular carcinoma cells inhibited the proliferation and invasion. In the process, expression level of transcription factor twist1 (twist‑related protein 1) was reduced, but no change at transcription level was observed...
July 7, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28688193/knockdown-of-lncrna-hulc-inhibits-proliferation-migration-invasion-and-promotes-apoptosis-by-sponging-mir-122-in-osteosarcoma
#11
Daliang Kong, Yang Wang
Osteosarcoma is a rare malignant bone tumor with high degree of malignancy. HULC (highly upregulated in liver cancer), a long noncoding RNA (lncRNA) was involved in hepatocellular carcinoma development and progression, but its underlying mechanism in osteosarcoma is unknown. The aim of this study was to explore the functional role of HULC in osteosarcoma. The study was conducted in human osteosarcoma cell lines and the expression of HULC in the cell lines was detected by qRT-PCR. Furthermore, the effects of HULC on tumorigenicity of osteosarcoma cells were evaluated by in vitro assays...
July 8, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28651931/upregulated-long-non-coding-rna-loc90784-promotes-cell-proliferation-and-invasion-and-is-associated-with-poor-clinical-features-in-hcc
#12
Jian-Hua Xu, Wei-Hua Chang, Hang-Wei Fu, Wei-Qun Shu, Tao Yuan, Ping Chen
A growing amount of literature has indicated that long non-coding RNAs (lncRNAs) are important factors in hepatocellular carcinoma (HCC) progression. However, the significance of lncRNAs in the progression and prognosis of liver cancer is largely unknown. In the present study, upregulated lncRNA LOC90784 was identified through integrative analysis of GSE58043 and GSE55191. Furthermore, associations between LOC90784 expression and the clinicopathological characteristics of patients were analyzed with a validated cohort 1 and the Cancer Genome Atlas (TCGA) cohort 2...
August 26, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28634076/lncrna-hulc-promotes-the-growth-of-hepatocellular-carcinoma-cells-via-stabilizing-cox-2-protein
#13
Haojun Xiong, Bo Li, Jintao He, Yijun Zeng, Yan Zhang, Fengtian He
Highly upregulated in liver cancer (HULC), a lncRNA overexpressed in hepatocellular carcinoma (HCC), has been demonstrated to be involved in the carcinogenesis and progression of HCC. However, the mechanisms of HULC promoting the abnormal growth of HCC cells are still not well elucidated. In the present study, we for the first time demonstrated that HULC promoted the growth of HCC cells through elevating COX-2 protein. Moreover, the study of the corresponding mechanism by which HULC upregulated COX-2 showed that HULC enhanced the level of ubiquitin-specific peptidase 22 (USP22), which decreased ubiquitin-mediated degradation of COX-2 protein by removing the conjugated polyubiquitin chains from COX-2 and finally stabilized COX2 protein...
August 26, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28611301/lncrna-svugp2-suppresses-progression-of-hepatocellular-carcinoma
#14
Jiangfeng Hu, Chenlin Song, Bensong Duan, Xiaoyan Zhang, Dongliang Li, Liang Zhu, Hengjun Gao
Numerous studies indicate that long noncoding RNAs (lncRNAs) are dysregulated in hepatocellular carcinoma (HCC) and might serve as potential diagnostic biomarkers and therapeutic targets of HCC. Therefore, it is interesting to globally identify the lncRNAs altered in HCC. In our study, we used microarray to profile the levels of lncRNAs and mRNAs in three pairs of HCC and their adjacent noncancerous samples. We found lncRNA-SVUGP2, which is a splice variant of the UGP2 gene, was down-regulated in HCC samples and correlates with a better prognosis in patients with HCC...
May 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28586153/mir-19a-affects-hepatocyte-autophagy-via-regulating-lncrna-nbr2-and-ampk-ppar%C3%AE-in-d-galn-lipopolysaccharide-stimulated-hepatocytes
#15
Yan-Min Liu, Jin-Hui Ma, Qing-Lei Zeng, Jun Lv, Xu-Hua Xie, Ya-Jie Pan, Zu-Jiang Yu
Objective This study aims to evaluate the potential involvement and regulatory mechanism of miR-19a in hepatocytes autophagy of acute liver failure (ALF). Material and methods The in vitro hepatocytes injury model of primary hepatocyte and hepatocytes line HL-7702 was established by D-galactosamine (D-GalN) and lipopolysaccharide (LPS) co-treatment. Relative expression level of miR-19a and NBR2 was determined by qRT-PCR. Protein expression of AMPK/PPARα and autophagy-related gene was determined by western blot...
June 6, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28536419/the-long-non-coding-rna-linc00152-is-essential-for-cell-cycle-progression-through-mitosis-in-hela-cells
#16
Linda Nötzold, Lukas Frank, Minakshi Gandhi, Maria Polycarpou-Schwarz, Matthias Groß, Manuel Gunkel, Nina Beil, Holger Erfle, Nathalie Harder, Karl Rohr, Jakob Trendel, Jeroen Krijgsveld, Thomas Longerich, Peter Schirmacher, Michael Boutros, Sylvia Erhardt, Sven Diederichs
In recent years, long non-coding RNA (lncRNA) research has identified essential roles of these transcripts in virtually all physiological cellular processes including tumorigenesis, but their functions and molecular mechanisms are poorly understood. In this study, we performed a high-throughput siRNA screen targeting 638 lncRNAs deregulated in cancer entities to analyse their impact on cell division by using time-lapse microscopy. We identified 26 lncRNAs affecting cell morphology and cell cycle including LINC00152...
May 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28508397/why-are-females-more-susceptible-to-cholestasis-induced-liver-injury-could-it-be-lncrna-h19
#17
EDITORIAL
Grace L Guo
No abstract text is available yet for this article.
May 15, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28469957/hbx-related-long-non-coding-rna-malat1-promotes-cell-metastasis-via-up-regulating-ltbp3-in-hepatocellular-carcinoma
#18
Zhouhua Hou, Xuwen Xu, Xiaoyu Fu, Shuhui Tao, Jiebin Zhou, Shuiping Liu, Deming Tan
Though it is widely known that hepatitis B virus X protein (HBx) is involved in the progression of hepatocellular carcinoma (HCC), the underlying mechanisms are not entirely clear. In recent years, metastasis associated with lung adenocarcinoma transcript 1 (MALAT1), which is an oncogenic long non-coding RNA (lncRNA), has been proved to be associated with many kinds of tumors, including liver cancer. In this study, we demonstrated that MALAT1 was involved in the HBx-mediated hepatocarcinogenesis. Firstly, we found that expression of MALAT1 was strongly up-regulated in HCC tissues and was directly proportional to the expression of HBx...
2017: American Journal of Cancer Research
https://www.readbyqxmd.com/read/28445086/long-noncoding-rna-highly-upregulated-in-liver-cancer-activates-p53-p21-pathway-and-promotes-nasopharyngeal-carcinoma-cell-growth
#19
Xue Jiang, Weiwei Liu
Dysfunction of lncRNA has been found in the nasopharyngeal carcinoma (NPC); however, the effect of lncRNA expression on NPC tumorigenesis as well as the molecular mechanism of lncRNA in the pathogenesis of NPC remain largely unknown. In this study, we showed that highly upregulated in liver cancer (HULC), the first identified lncRNA in hepatocellular carcinoma, is highly expressed in NPC patients and correlated with a poor prognosis of cancer patients. Overexpressed HULC promotes NPC cell growth. Downregulation of HULC activated p53 and induced the increased expression of p21, which finally caused cell cycle arrest and cell apoptosis...
July 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28430582/lncrna-chrf-induced-mir-489-loss-promotes-metastasis-of-colorectal-cancer-via-twist1-emt-signaling-pathway
#20
Youmao Tao, Tao Han, Tao Zhang, Chong Ma, Caixia Sun
microRNA-489 (miR-489) is a novel cancer-related miRNAs and functions as a tumor suppressor in human cancers. While, the clinical significance of miR-489 and its role in colorectal cancer (CRC) remain rarely known. Here, we found that the levels of miR-489 in CRC tissues were significantly lower than those in matched tumor-adjacent tissues. Furthermore, decreased levels of miR-489 also observed in CRC cell lines compared to HIEC cells. Clinicopathological analysis revealed that miR-489 underexpression was positively correlated with advanced pT stage, pN stage and AJCC stage...
May 30, 2017: Oncotarget
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