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Kyung Mi Kim, Ki Hwa Lee, Yong Han Kim, Myoung Jin Ko, Jae-Wook Jung, Eunsu Kang
OBJECTIVE: A prospective, double-blind, randomized controlled trial to compare the effect of preoperative midazolam or ketamine on the incidence of emergence agitation (EA) following sevoflurane anaesthesia in children. METHODS: Paediatric patients (2-6 years old) undergoing ophthalmic surgery were allocated to receive premedication with either 0.1 mg/kg midazolam or 1 mg/kg ketamine. Incidence of EA and postoperative pain scores were recorded at 10-min intervals in the postanaesthetic care unit (PACU)...
April 2016: Journal of International Medical Research
Yasushi Kishimoto, Moritoshi Hirono, Ryuichiro Atarashi, Suehiro Sakaguchi, Tohru Yoshioka, Shigeru Katamine, Yutaka Kirino
Mice lacking the prion protein (PrP(C)) gene (Prnp), Ngsk Prnp (0/0) mice, show late-onset cerebellar Purkinje cell (PC) degeneration because of ectopic overexpression of PrP(C)-like protein (PrPLP/Dpl). Because PrP(C) is highly expressed in cerebellar neurons (including PCs and granule cells), it may be involved in cerebellar synaptic function and cerebellar cognitive function. However, no studies have been conducted to investigate the possible involvement of PrP(C) and/or PrPLP/Dpl in cerebellum-dependent discrete motor learning...
2013: PloS One
Hiroyuki Moriuchi, Hideaki Masuzaki, Hiroshi Doi, Shigeru Katamine
No abstract text is available yet for this article.
February 2013: Pediatric Infectious Disease Journal
Yoshitaka Yamaguchi, Hironori Miyata, Keiji Uchiyama, Akira Ootsuyama, Sachiko Inubushi, Tsuyoshi Mori, Naomi Muramatsu, Shigeru Katamine, Suehiro Sakaguchi
Accumulating lines of evidence indicate that the N-terminal domain of prion protein (PrP) is involved in prion susceptibility in mice. In this study, to investigate the role of the octapeptide repeat (OR) region alone in the N-terminal domain for the susceptibility and pathogenesis of prion disease, we intracerebrally inoculated RML scrapie prions into tg(PrPΔOR)/Prnp(0/0) mice, which express mouse PrP missing only the OR region on the PrP-null background. Incubation times of these mice were not extended. Protease-resistant PrPΔOR, or PrP(Sc)ΔOR, was easily detectable but lower in the brains of these mice, compared to that in control wild-type mice...
2012: PloS One
Toshiya Kubota, Yuta Hamazoe, Shuhei Hashiguchi, Daisuke Ishibashi, Kazuyuki Akasaka, Noriyuki Nishida, Shigeru Katamine, Suehiro Sakaguchi, Ryota Kuroki, Toshihiro Nakashima, Kazuhisa Sugimura
We prepared β-sheet-rich recombinant full-length prion protein (β-form PrP) (Jackson, G. S., Hosszu, L. L., Power, A., Hill, A. F., Kenney, J., Saibil, H., Craven, C. J., Waltho, J. P., Clarke, A. R., and Collinge, J. (1999) Science 283, 1935-1937). Using this β-form PrP and a human single chain Fv-displaying phage library, we have established a human IgG1 antibody specific to β-form but not α-form PrP, PRB7 IgG. When prion-infected ScN2a cells were cultured with PRB7 IgG, they generated and accumulated PRB7-binding granules in the cytoplasm with time, consequently becoming apoptotic cells bearing very large PRB7-bound aggregates...
April 20, 2012: Journal of Biological Chemistry
Ikuro Takakura, Kohtaro Miyazawa, Takashi Kanaya, Wataru Itani, Kouichi Watanabe, Shyuichi Ohwada, Hitoshi Watanabe, Tetsuya Hondo, Michael T Rose, Tsuyoshi Mori, Suehiro Sakaguchi, Noriyuki Nishida, Shigeru Katamine, Takahiro Yamaguchi, Hisashi Aso
Transmissible spongiform encephalopathies are fatal neurodegenerative diseases. Infection by the oral route is assumed to be important, although its pathogenesis is not understood. Using prion protein (PrP) knockout mice, we investigated the sequence of events during the invasion of orally administered PrPs through the intestinal mucosa and the spread into lymphoid tissues and the peripheral nervous system. Orally administered PrPs were incorporated by intestinal epitheliocytes in the follicle-associated epithelium and villi within 1 hour...
September 2011: American Journal of Pathology
Kohtaro Miyazawa, Takashi Kanaya, Ikuro Takakura, Sachi Tanaka, Tetsuya Hondo, Hitoshi Watanabe, Michael T Rose, Haruki Kitazawa, Takahiro Yamaguchi, Shigeru Katamine, Noriyuki Nishida, Hisashi Aso
Transmissible spongiform encephalopathies (TSE), including bovine spongiform encephalopathy (BSE), are fatal neurodegenerative disorders in humans and animals. BSE appears to have spread to cattle through the consumption of feed contaminated with BSE/scrapie agents. In the case of an oral infection, the agents have to cross the gut-epithelial barrier. We recently established a bovine intestinal epithelial cell line (BIE cells) that can differentiate into the M cell type in vitro after lymphocytic stimulation (K...
December 2010: Journal of Virology
Khaleque Newaz Khan, Michio Kitajima, Koichi Hiraki, Naohiro Yamaguchi, Shigeru Katamine, Toshifumi Matsuyama, Masahiro Nakashima, Akira Fujishita, Tadayuki Ishimaru, Hideaki Masuzaki
To test the hypothesis that bacterial contamination of menstrual blood could be a local biologic event in the development of endometriosis, menstrual blood was cultured and bacterial endotoxin was measured in menstrual blood and peritoneal fluid. Our results suggest that compared with control women, higher colony formation of Escherichia coli in menstrual blood and endotoxin levels in menstrual fluid and peritoneal fluid in women with endometriosis may promote Toll-like receptor 4-mediated growth of endometriosis...
December 2010: Fertility and Sterility
Takako Shimada, Naohiro Yamaguchi, Noriyuki Nishida, Kentaro Yamasaki, Kiyonori Miura, Shigeru Katamine, Hideaki Masuzaki
OBJECTIVES: The squamous cell carcinoma antigen is considered the most accurate serologic tumor marker for uterine cervical carcinoma. However, serum squamous cell carcinoma antigen levels were found to correlate significantly with clinical severity of atopic dermatitis and chronic renal failure. The present study was conducted in patients with human papillomavirus 16 DNA-positive uterine cervical cancer to determine the plasma level of human papillomavirus 16 DNA and the diagnostic values of plasma human papillomavirus DNA in these patients...
May 2010: Japanese Journal of Clinical Oncology
Cheng-bin Zhou, Jian Zhuang, Shu-sheng Wen, Zhou-cuo Qi, Li-ming Yao
OBJECTIVE: To summarize the anesthetic management in fetal lamb cardiac bypass. METHODS: Five ewes at 120-140 days of gestation were anesthetized intramuscularly with katamine hydrochloride, intubated and ventilated with a respirator. Anesthesia was maintained with fentanyl and vecuronium. Lactated Ringer's solution and magnesium sulfate were infused to maintain the mean blood pressure (MAP) over 70 mmHg and uterine relaxation. The fetal lambs received anesthesia with fentanyl and vecuronium intramuscularly via the uterine wall...
December 2009: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
Aiko Fujihara, Ryuichiro Atarashi, Takayuki Fuse, Kaori Ubagai, Takehiro Nakagaki, Naohiro Yamaguchi, Daisuke Ishibashi, Shigeru Katamine, Noriyuki Nishida
Abnormal forms of prion protein (PrP(Sc)) accumulate via structural conversion of normal PrP (PrP(C)) in the progression of transmissible spongiform encephalopathy. Under cell-free conditions, the process can be efficiently replicated using in vitro PrP(Sc) amplification methods, including protein misfolding cyclic amplification. These methods enable ultrasensitive detection of PrP(Sc); however, there remain difficulties in utilizing them in practice. For example, to date, several rounds of protein misfolding cyclic amplification have been necessary to reach maximal sensitivity, which not only take several weeks, but also result in an increased risk of contamination...
May 2009: FEBS Journal
Daisuke Yoshikawa, Naohiro Yamaguchi, Daisuke Ishibashi, Hitoki Yamanaka, Nobuhiko Okimura, Yoshitaka Yamaguchi, Tsuyoshi Mori, Hironori Miyata, Kazuto Shigematsu, Shigeru Katamine, Suehiro Sakaguchi
Prion protein-like protein/doppel is neurotoxic, causing ataxia and Purkinje cell degeneration in mice, whereas prion protein antagonizes doppel-induced neurodegeneration. Doppel is homologous to the C-terminal half of prion protein but lacks the amino acid sequences corresponding to the N-terminal half of prion protein. We show here that transgenic mice expressing a fusion protein consisting of the N-terminal half, corresponding to residues 1-124, of prion protein and doppel in neurons failed to develop any neurological signs for up to 730 days in a background devoid of prion protein...
August 29, 2008: Journal of Biological Chemistry
Kazuo Kuwata, Noriyuki Nishida, Tomoharu Matsumoto, Yuji O Kamatari, Junji Hosokawa-Muto, Kota Kodama, Hironori K Nakamura, Kiminori Kimura, Makoto Kawasaki, Yuka Takakura, Susumu Shirabe, Jiro Takata, Yasufumi Kataoka, Shigeru Katamine
Prion proteins are key molecules in transmissible spongiform encephalopathies (TSEs), but the precise mechanism of the conversion from the cellular form (PrP(C)) to the scrapie form (PrP(Sc)) is still unknown. Here we discovered a chemical chaperone to stabilize the PrP(C) conformation and identified the hot spots to stop the pathogenic conversion. We conducted in silico screening to find compounds that fitted into a "pocket" created by residues undergoing the conformational rearrangements between the native and the sparsely populated high-energy states (PrP*) and that directly bind to those residues...
July 17, 2007: Proceedings of the National Academy of Sciences of the United States of America
Tsuyoshi Mori, Ryozo Moriuchi, Eiko Okazaki, Kenji Yamada, Shigeru Katamine
We identified RECK, a membrane-anchored glycoprotein negatively regulating the activities of MMPs, as a molecule interacting with Tgat oncoprotein consisting of RhoGEF domain and the unique C-terminal 15 amino acids. The Tgat increased the invasive potential of NIH3T3 cells expressing endogenous mouse RECK and this effect was partially inhibited by the co-expression of human RECK. On the contrary, the expression of exogenous human RECK in HT1080 cell line lacking the endogenous RECK expression reduced its invasive activity, which was recovered by the Tgat co-expression...
April 20, 2007: Biochemical and Biophysical Research Communications
Kenji Yamada, Ryozo Moriuchi, Tsuyoshi Mori, Eiko Okazaki, Tomoko Kohno, Takeshi Nagayasu, Toshifumi Matsuyama, Shigeru Katamine
Constitutive activity of NF-kappaB is associated with various human cancers including adult T-cell leukemia (ATL). In this study, we have found Tgat that activates NF-kappaB by screening a cDNA expression library derived from ATL cells. We previously identified Tgat as the oncogene, which consists of the Rho-guanine nucleotide exchange factor (Rho-GEF) domain and the unique C-terminal region, as a consequence of alternative splicing of the Trio transcript. Tgat activated the IKK activity by binding with the IkappaB kinase (IKK) complex...
March 30, 2007: Biochemical and Biophysical Research Communications
Kohtaro Miyazawa, Takashi Kanaya, Sachi Tanaka, Ikuro Takakura, Kouichi Watanabe, Shyuichi Ohwada, Haruki Kitazawa, Michael T Rose, Suehiro Sakaguchi, Shigeru Katamine, Takahiro Yamaguchi, Hisashi Aso
The gastrointestinal tract is thought to be the main site of entry for the pathological isoform of the prion protein (PrP(Sc)). Prion diseases are believed to result from a conformational change of the cellular prion protein (PrP(c)) to PrP(Sc). Therefore, PrP(c) expression is a prerequisite for the infection and spread of the disease to the central nervous system. However, the distribution of PrP(c) in the gut is still a matter of controversy. We therefore investigated the localization of PrP(c) in the bovine and murine small intestine...
March 2007: Histochemistry and Cell Biology
Daisuke Ishibashi, Hitoki Yamanaka, Naohiro Yamaguchi, Daisuke Yoshikawa, Risa Nakamura, Nobuhiko Okimura, Yoshitaka Yamaguchi, Kazuto Shigematsu, Shigeru Katamine, Suehiro Sakaguchi
Host tolerance to endogenous prion protein (PrP) has hampered the development of prion vaccines as PrP is a major component of prions. Indeed, we show that immunization of mice with mouse recombinant PrP elicited no prophylactic effect against a mouse-adapted prion. However, interestingly, mice immunized with recombinant bovine PrP developed the disease significantly later than non-immunized mice after inoculation of a mouse prion. Sheep recombinant PrP exhibited variable prophylactic effects. Mouse recombinant PrP stimulated only very weak antibody responses...
January 22, 2007: Vaccine
Daisuke Yoshikawa, Juraj Kopacek, Naohiro Yamaguchi, Daisuke Ishibashi, Hitoki Yamanaka, Yoshitaka Yamaguchi, Shigeru Katamine, Suehiro Sakaguchi
We and others previously showed that, in some lines of prion protein (PrP)-knockout mice, the downstream PrP-like protein (PrPLP/Dpl) was abnormally expressed in brains partly due to impaired cleavage/polyadenylation of the residual PrP promoter-driven pre-mRNA despite the presence of a poly(A) signal. In this study, we newly established an in vitro transient transfection system in which abnormal expression of PrPLP/Dpl can be visualized by expression of the green fluorescence protein, EGFP, in cultured cells...
January 15, 2007: Gene
Ryuichiro Atarashi, Valerie L Sim, Noriyuki Nishida, Byron Caughey, Shigeru Katamine
Although the protein-only hypothesis proposes that it is the conformation of abnormal prion protein (PrP(Sc)) that determines strain diversity, the molecular basis of strains remains to be elucidated. In the present study, we generated a series of mutations in the normal prion protein (PrP(C)) in which a single glutamine residue was replaced with a basic amino acid and compared their abilities to convert to PrP(Sc) in cultured neuronal N2a58 cells infected with either the Chandler or 22L mouse-adapted scrapie strain...
August 2006: Journal of Virology
Katsuya Satoh, Susumu Shirabe, Hiroto Eguchi, Akira Tsujino, Katsumi Eguchi, Akira Satoh, Mitsuhiro Tsujihata, Masami Niwa, Shigeru Katamine, Saiko Kurihara, Hidenori Matsuo
1. Sporadic Creutzfeldt-Jakob disease (CJD) is a rapidly progressive and fatal disease. Patients with CJD usually become akinetic mutism within approximately 6 months. In addition, clinical signs and symptoms at early stage of sporadic CJD may not be easy to distinguish from other neurodegenerative diseases by neurological findings. However, diagnostic biochemical parameters including 14-3-3 protein, S100, neuron-specific enorase in cerebrospinal fluid (CSF) have been used as diagnostic markers, elevated titers of these markers can also be observed in CSF in other neurodegenerative diseases...
February 2006: Cellular and Molecular Neurobiology
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