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https://www.readbyqxmd.com/read/28441544/enrichment-of-resolving-power-improves-ion-peak-quantification-on-a-lipidomics-platform
#1
Kosuke Saito, Yasuo Ohno, Yoshiro Saito
In this study, we delineated the importance of MS resolving power on the ion-peak quantification of lipids using an Orbitrap Fusion instrument and established a liquid chromatography-based, high-performance lipidomics platform. The ion-peak recognition of several lipids in human plasma, such as LPC(15:0), LPE(22:5), and PC(35:0), was clearly improved by increasing the MS resolving power. In addition, we evaluated the impact of resolving power on the quantitative detection of lipids by automatic ion-peak recognition with calculation of the coefficient of variance (CV)...
April 13, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28441017/equilibration-of-micelle-polyelectrolyte-complexes-mechanistic-differences-between-static-and-annealed-charge-distributions
#2
Jennifer E Laaser, Michael McGovern, Yaming Jiang, Elise Lohmann, Theresa M Reineke, David C Morse, Kevin D Dorfman, Timothy P Lodge
The role of charge density and charge annealing in polyelectrolyte complexation was investigated through systematic comparison of two micelle-polyelectrolyte systems. First, poly(dimethylaminoethyl methacrylate)-block-poly(styrene) (PDMAEMA-b-PS) micelles were complexed with poly(styrenesulfonate) (PSS) at pH values above and below the pKa of PDMAEMA to investigate the role of charge annealing in the complexation process. Second, complexes of poly(DMAEMA-stat-oligo(ethylene glycol) methyl ether methacrylate)-block-poly(styrene) (P(DMAEMA-stat-OEGMA)-b-PS) micelles with the same PSS at low pH were used to investigate how the complexation process differs when the charged sites are in fixed positions along the polymer chains...
April 25, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28440612/association-between-xrcc1-and-wrn-as-genetic-markers-of-stability-and-susceptibility-to-cancer-in-patients-with-hiv-aids-and-cancer-a-cross-sectional-study
#3
Gabriel de Carvalho Maldonado, Orlando Nascimento Terra Júnior, Adriano Arnóbio, Guilherme Rohem Alfradique, Maria Helena Ornellas, Roberto Irineu da Silva, Dirce Bonfim de Lima
Background: HIV-induced immunodeficiency has been implicated as a key factor for risk of cancer. Neoplasia is considered to result from accumulation of damage to the genome. Polymorphisms in repair genes, such as the XRCC1 and WRN, have been associated with susceptibility to development of cancer in patients with HIV/AIDS. The aim of this study was to analyze the frequency of polymorphisms in XRCC1 (Arg399Gln) and WRN (Cys1367Arg) in patients with HIV/AIDS with or without cancer. Materials and Methods: Genotyping for analysis of polymorphisms was carried out by PCR (Polymerase Chain Reaction) and RFLP (Restriction Fragment Length Polymorphism)...
March 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28440415/udp-and-ntf2-are-the-most-consistently-expressed-genes-in-panax-ginseng-roots-at-different-growth-stages
#4
Meichen Liu, Qun Wang, Hongmei Xie, Shichao Liu, Siming Wang, Hui Zhang, Yu Zhao
Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis relies on normalization against a consistently expressed reference gene. However, it has been reported that reference gene expression levels often vary markedly between samples as they are usually selected based solely on convention. The advent of RNA sequencing technology offers the opportunity to select reference genes with the least variability in steady‑state transcript levels. To identify the most consistently stable genes, which are a prerequisite for obtaining reliable gene expression data, the present study analyzed transcriptomes from six Panax ginseng transcriptome data sets, representing six growth stages, and selected 21 candidate reference genes for screening using RT‑qPCR...
April 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28440201/investigation-of-the-ph-dependent-cytotoxicity-of-paclitaxel-conjugated-gold-nanoparticles
#5
Zahrah Alhalili, Nur Hikmatul Auliya, Barbara Sandersonb, J G Shapter
Breast cancer, apart from skin cancer, is the most common cancer among women accounting for nearly 1 in 3 cancers and the second leading cause of cancer-related death among women after lung cancer. Finding new approaches to treat such cancers is critically important. OBJECTIVE: This work investigated the ability of a simple system based on paclitaxel conjugated gold nanoparticles (AuNP) to induce efficient cytotoxicity against T47D breast cancerous cells at different pH values. METHOD: The synthesis and characterization of nanoparticles using two approaches is presented...
April 24, 2017: Pharmaceutical Nanotechnology
https://www.readbyqxmd.com/read/28439924/prevalence-and-predictors-of-thyroid-functional-abnormalities-in-newly-diagnosed-al-amyloidosis
#6
E Muchtar, D S Dean, A Dispenzieri, D Dingli, F K Buadi, M Q Lacy, S R Hayman, P Kapoor, N Leung, S Russell, J A Lust, Yi Lin, R Warsame, W Gonsalves, T V Kourelis, R S Go, R Chakraborty, S Zeldenrust, R A Kyle, S Vincent Rajkumar, S K Kumar, M A Gertz
BACKGROUND: Data on the effect of systemic immunoglobulin light chain amyloidosis (AL amyloidosis) on thyroid function are limited. OBJECTIVE: To assess the prevalence of hypothyroidism in AL amyloidosis patients and determine its predictors. METHODS: 1142 newly diagnosed AL amyloidosis patients were grouped based on the thyroid-stimulating hormone (TSH) measurement at diagnosis: hypothyroid group (TSH above upper normal reference; >5 mIU L(-1) ; n = 217, 19% of study participants) and euthyroid group (n = 925, 81%)...
April 24, 2017: Journal of Internal Medicine
https://www.readbyqxmd.com/read/28439316/epigenetic-assays-for-chemical-biology-and-drug-discovery
#7
REVIEW
Sheraz Gul
The implication of epigenetic abnormalities in many diseases and the approval of a number of compounds that modulate specific epigenetic targets in a therapeutically relevant manner in cancer specifically confirms that some of these targets are druggable by small molecules. Furthermore, a number of compounds are currently in clinical trials for other diseases including cardiovascular, neurological and metabolic disorders. Despite these advances, the approved treatments for cancer only extend progression-free survival for a relatively short time and being associated with significant side effects...
2017: Clinical Epigenetics
https://www.readbyqxmd.com/read/28437552/bcr-abl1-transcript-types-showed-distinct-laboratory-characteristics-in-patients-with-chronic-myeloid-leukemia
#8
A P Vasconcelos, I F Azevedo, F C B C Melo, W B Neves, A C A C Azevedo, R A M Melo
In chronic myeloid leukemia (CML) two main types of messenger RNA (e14a2 and e13a2) can be produced by BCR-ABL1 gene rearrangement. Due to conflicting results, the clinical value of these transcripts remains controversial. The aim of this study was to identify associations of e14a2 and e13a2 transcripts with laboratory variables and also the response to treatment. This study included 203 adult patients with CML treated with Imatinib as first-line drug in a reference hematology center in Northeast Brazil. Clinical and laboratory data were obtained after informed consent...
April 20, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28436387/predictive-value-of-microrna-143-in-evaluating-the-prognosis-of-patients-with-hepatocellular-carcinoma
#9
Jian Zhang, Hai Lin, Xiao-Ying Wang, Dong-Qin Zhang, Jian-Xia Chen, Yan Zhuang, Xiao-Li Zheng
OBJECTIVE: The study aims to evaluate the predictive value of microRNA-143 (miR-143) for the prognosis of patients with hepatocellular carcinoma (HCC). METHODS: Between October 2010 and October 2012, 131 HCC patients were selected as a case group; meanwhile, 122 healthy controls were enrolled as a control group. The miR-143 expression in serum was detected by quantitative real-time polymerase chain reaction (qRT-PCR). These HCC patients were divided into the high miR-143 expression group and the low miR-143 expression group based on the threshold of receiver operating characteristic (ROC) curve...
March 31, 2017: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/28435731/crystal-structure-and-absolute-configuration-of-4s-5r-6s-4-5-6-trihy-droxy-3-methyl-cyclo-hex-2-enone-gabosine-h
#10
Gaurao D Tibhe, Mario A Macías, Enrique Pandolfi, Valeria Schapiro, Leopoldo Suescun
The mol-ecule of the title keto carbasugar, C7H10O4, is formed by a cyclo-hexene skeleton with an envelope conformation, substituted by carbonyl, methyl and hydroxyl groups. The crystal structure is controlled mainly by a combination of strong O-H⋯O and weak C-H⋯O hydrogen bonds, forming nearly perpendicular chains running parallel to the [110] and [-110] directions. This perpendicularity is caused by a tetra-gonal pseudosymmetry influenced by the similarity between the a and b axes, the value of 90.9770 (10)° of the β angle and the action of a 21 screw axis, which transform each chain into its corresponding nearly orthogonal one...
April 1, 2017: Acta Crystallographica. Section E, Crystallographic Communications
https://www.readbyqxmd.com/read/28435526/rita-mimics-synthesis-and-mechanistic-evaluation-of-asymmetric-linked-trithiazoles
#11
Adrian L Pietkiewicz, Yuqi Zhang, Marwa N Rahimi, Michael Stramandinoli, Matthew Teusner, Shelli R McAlpine
The established cytotoxic agent RITA contains a thiophene-furan-thiophene backbone and two terminal alcohol groups. Herein we investigate the effect of using thiazoles as the backbone in RITA-like molecules and modifying the terminal groups of these trithiazoles, thereby generating 41 unique structures. Incorporating side chains with varied steric bulk allowed us to investigate how size and a stereocenter impacted biological activity. Subjecting compounds to growth inhibition assays on HCT-116 cells showed that the most potent compounds 7d, 7e, and 7h had GI50 values of 4...
April 13, 2017: ACS Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28434809/real-world-performance-of-the-new-us-hiv-testing-algorithm-in-medical-settings
#12
Kara G Marson, Robert Marlin, Phong Pham, Stephanie E Cohen, Diane Jones, Marguerite Roemer, Philip J Peters, Barbara Haller, Christopher D Pilcher
BACKGROUND: Our medical center laboratory recently adapted its 24/7, two-hourly testing program to use an ARCHITECT-Multispot-viral load (AR-MS-VL) algorithm in place of a previous rapid test-immunofluorescence (RT-IF) algorithm. OBJECTIVES: We evaluated screening test performance, acute case detection, turnaround time and ability to resolve HIV status under the new algorithm. STUDY DESIGN: We considered consecutive HIV tests from January to November 2015...
April 7, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28434722/prediction-of-milk-fatty-acid-content-with-mid-infrared-spectroscopy-in-canadian-dairy-cattle-using-differently-distributed-model-development-sets
#13
A Fleming, F S Schenkel, J Chen, F Malchiodi, V Bonfatti, R A Ali, B Mallard, M Corredig, F Miglior
The fatty acid profile of milk is a prevailing issue due to the potential negative or positive effects of different fatty acids to human health and nutrition. Mid-infrared spectroscopy can be used to obtain predictions of otherwise costly fatty acid phenotypes in a widespread and rapid manner. The objective of this study was to evaluate the prediction of fatty acid content for the Canadian dairy cattle population from mid-infrared spectral data and to compare the results produced by altering the partial least squares (PLS) model development set used...
April 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28433770/preparation-of-low-digestible-and-viscoelastic-tigernut-cyperus-esculentus-starch-by-bacillus-acidopullulyticus-pullulanase
#14
Xiaolei Li, Jingchao Fu, Yujuan Wang, Fumin Ma, Dan Li
Tigernut starch is an underutilized food resource. In this study, pullulanase (PUL) hydrolysis was used to change its physiochemical properties for different food applications. The content of low digestible fractions, resistant starch and slow digestible starch, in PUL modified tigernut starch significantly increased from 2.03% to 25.08% (P <0.05) using 120U/g starch of PUL in the debranching reaction. The paste or dispersion of PUL modified tigernut starch had a significantly decreased viscoelasticity (P <0...
April 19, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28433512/effect-of-1-2-3-triazole-salts-non-classical-bioisosteres-of-miltefosine-on-leishmania-amazonensis
#15
Pedro H F Stroppa, Luciana M R Antinarelli, Arturene M L Carmo, Jacy Gameiro, Elaine S Coimbra, Adilson D da Silva
Here, we report the effect of new non-classical bioisosteres of miltefosine on Leishmania amazonensis. Fifteen compounds were synthesized and the compound dhmtAc, containing an acetate anion, a side chain of 10 carbon atoms linked to N-1 and a methyl group linked to N-3, showed high and selective biological activity against L. amazonensis. On the intracellular amastigotes, stages of the parasite related to human disease, the IC50 values were near or similar to the 1.0μM (0.9, 0.8 and 1.0μM on L. amazonensis-WT, and two transgenic L...
March 28, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28433472/the-influence-of-platelet-rich-plasma-on-synovial-fluid-volumes-protein-concentrations-and-severity-of-pain-in-patients-with-knee-osteoarthritis
#16
Carl P C Chen, Chih-Hsiu Cheng, Chih-Chin Hsu, Hsin-Chin Lin, Yi-Rong Tsai, Jean-Lon Chen
Knee pain is commonly seen in orthopedic and rehabilitation outpatient clinical settings. Patients with knee osteoarthritis (OA) are often complicated with joint soreness, swelling, weakness, and pain. These complaints are often caused by the excessive amount of synovial fluid (SF) accumulated in the bursae around the knee joint. This study was aimed to evaluate the effectiveness of platelet rich plasma (PRP) in treating patients with minor to moderate knee osteoarthritis (OA) combined with supra-patellar bursitis using a proteomic approach and clinical evaluation tool...
April 19, 2017: Experimental Gerontology
https://www.readbyqxmd.com/read/28431371/past-present-and-future-land-use-changes-and-their-impact-on-water-balance
#17
Sananda Kundu, Deepak Khare, Arun Mondal
Landuse change influences the water balance of a region affecting the available water along with the change in the evapotranspiration (ET). The major objectives of this study are to assess the landuse change and its impact on the water balance of the study area, which is a part of the Narmada river basin in Madhya Pradesh, India. Landuse changes of 1990, 2000 and 2011 have been analyzed and the Markov Chain model has been used to predict decadal change of 2020, 2030, 2040 and 2050 landuse. The influence of the past, present and future landuse change on water balance has been analyzed with the SWAT (Soil and Water Analysis Tool) model in the study area...
April 17, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28430524/mirna-related-polymorphisms-in-mir-423-rs6505162-and-pex6-rs1129186-and-risk-of-esophageal-squamous-cell-carcinoma-in-an-iranian-cohort
#18
Ziba Nariman-Saleh-Fam, Milad Bastami, Mohammad Hossein Somi, Farkhondeh Behjati, Yaser Mansoori, Abdolreza Daraei, Zahra Saadatian, Lida Nariman-Saleh-Fam, Habibollah Mahmoodzadeh, Yashar Makhdoumi, Fatemeh Varshoee Tabrizi, Bahador Ebrahimi-Sharif, Azam Hezarian, Shahnaz Naghashi, Mohammad Reza Abbaszadegan, Javad Tavakkoly-Bazzaz
AIMS: Iran is located in the Asian esophageal cancer belt. It is a high-risk region for esophageal squamous cell carcinoma (ESCC). The extent to which genetic components, especially variants within miRNAs or their binding sites, contribute to risk of ESCC in the region is not yet fully understood. Herein, tests were done on an Iranian cohort to evaluate the association of miRNA-related polymorphisms in miR-423 (rs6505162) and peroxisomal biogenesis factor 6 (PEX6) (rs1129186 within a miR-149-5p-binding site) with risk of ESCC risk...
April 21, 2017: Genetic Testing and Molecular Biomarkers
https://www.readbyqxmd.com/read/28428721/proposed-criteria-to-differentiate-heterogeneous-eosinophilic-gastrointestinal-disorders-of-the-esophagus-including-eosinophilic-esophageal-myositis
#19
Hiroki Sato, Nao Nakajima, Kazuya Takahashi, Go Hasegawa, Ken-Ichi Mizuno, Satoru Hashimoto, Satoshi Ikarashi, Kazunao Hayashi, Yutaka Honda, Junji Yokoyama, Yuichi Sato, Shuji Terai
AIM: To define clinical criteria to differentiate eosinophilic gastrointestinal disorder (EoGD) in the esophagus. METHODS: Our criteria were defined based on the analyses of the clinical presentation of eosinophilic esophagitis (EoE), subepithelial eosinophilic esophagitis (sEoE) and eosinophilic esophageal myositis (EoEM), identified by endoscopy, manometry and serum immunoglobulin E levels (s-IgE), in combination with histological and polymerase chain reaction analyses on esophageal tissue samples...
April 7, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28428133/epidemiological-study-of-common-diseases-and-their-risk-factors-in-camels-in-south-punjab-pakistan
#20
Aneela Zameer Durrani, Zubair Bashir, Imran Rasheed, Noor-Ul-Ain Sarwar
Bacteriological study of mastitis along with common blood protozoan diseases were studied in dromedary camels in Cholistan, Dera Ismail Khan and Rahim Yar Khan districts in South Punjab, Pakistan. For this purpose 300 camels were sampled randomly at different common grazing and watering point. For study of blood parasites clinically suspected and apparently healthy camels, 150 each, were sampled. An overall prevalence of 15%and 5% was recorded for trypanosomiasis and Anaplasmosis respectively. Trypanosoma evansi was identified with 280 bp product on polymerase chain reaction test...
April 17, 2017: Microbial Pathogenesis
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