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https://www.readbyqxmd.com/read/28343171/circulating-free-light-chain-measurement-in-the-diagnosis-prognostic-assessment-and-evaluation-of-response-of-al-amyloidosis-comparison-of-freelite-and-n-latex-flc-assays
#1
Giovanni Palladini, Arnaud Jaccard, Paolo Milani, David Lavergne, Andrea Foli, Sebastien Bender, Francesca Lavatelli, Tiziana Bosoni, Veronica Valentini, Laura Pirolini, Giovanni Ferraro, Marco Basset, Francesca Russo, Mario Nuvolone, Riccardo Albertini, Michel Cogne, Giampaolo Merlini
BACKGROUND: The measurement of circulating free light chain (FLC) is essential in the diagnosis, prognostic stratification and evaluation of response to therapy in light chain (AL) amyloidosis. For more than 10 years, this has been done with an immunonephelometric assay based on polyclonal antibodies (Freelite), and cutoffs for staging and response assessment have been validated with this method. Recently, a new assay based on monoclonal antibodies (N latex FLC) has been marketed in Europe...
March 27, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28342317/high-androgen-receptor-mrna-expression-is-independently-associated-with-prolonged-cancer-specific-and-recurrence-free-survival-in-stage-t1-bladder-cancer
#2
Danijel Sikic, Johannes Breyer, Arndt Hartmann, Maximilian Burger, Philipp Erben, Stefan Denzinger, Markus Eckstein, Robert Stöhr, Sven Wach, Bernd Wullich, Bastian Keck, Ralph M Wirtz, Wolfgang Otto
INTRODUCTION: High-risk non-muscle-invasive bladder cancer (NMIBC) remains challenging given the high probability of progression. Given that the androgen receptor (AR) has been discussed as a possible factor in the development and progression of bladder cancer, we investigated the predictive value of AR in stage pT1 NMIBC. MATERIALS AND METHODS: We retrospectively analyzed the clinical data and AR mRNA expression in 296 patients with stage pT1 NMIBC who underwent a transurethral resection of the bladder...
March 22, 2017: Translational Oncology
https://www.readbyqxmd.com/read/28339756/molecular-diagnosis-of-pneumocystis-pneumonia-in-dogs
#3
Patrizia Danesi, Silvia Ravagnan, Lynelle R Johnson, Tommaso Furlanello, Adelaide Milani, Patricia Martin, Susan Boyd, Matthew Best, Bradley Galgut, Peter Irwin, Paul J Canfield, Mark B Krockenberger, Catriona Halliday, Wieland Meyer, Richard Malik
Pneumocystis pneumonia (PCP) is a life-threatening fungal disease that can occur in dogs. The aim of this study was to provide a preliminary genetic characterisation of Pneumocystis carinii f.sp.'canis' (P. canis) in dogs and thereby develop a reliable molecular protocol to definitively diagnose canine PCP. We investigated P. canis in a variety of lung specimens from dogs with confirmed or strongly suspected PCP (Group 1, n = 16), dogs with non-PCP lower respiratory tract problems (Group 2, n = 65) and dogs not suspected of having PCP or other lower respiratory diseases (Group 3, n = 11)...
February 23, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/28339481/proteomic-analysis-of-injured-storage-roots-in-cassava-manihot-esculenta-crantz-under-postharvest-physiological-deterioration
#4
Yuling Qin, Astride Stéphanie Mouafi Djabou, Feifei An, Kaimian Li, Zhaogui Li, Long Yang, Xiaojing Wang, Songbi Chen
Postharvest physiological deterioration (PPD) is a global challenge in the improvement of cassava value chain. However, how to reduce cassava spoilage and reveal the mechanism of injured cassava storage roots in response to PPD were poorly understood. In the present study, we investigated the activities of antioxidant enzymes of cassava injured storage roots in PPD-susceptible (SC9) and PPD-tolerant (QZ1) genotypes at the time-points from 0h to 120h, and further analyzed their proteomic changes using two-dimensional electrophoresis (2-DE) in combination with MALDI-TOF-MS/MS...
2017: PloS One
https://www.readbyqxmd.com/read/28339190/luminescent-zn-ii-coordination-polymers-for-highly-selective-sensing-of-cr-iii-and-cr-vi-in-water
#5
Tian-Yi Gu, Ming Dai, David James Young, Zhi-Gang Ren, Jian-Ping Lang
Three photoluminescent zinc coordination polymers (CPs), {[Zn2(tpeb)2(2,5-tdc)(2,5-Htdc)2]·2H2O}n (1), {[Zn2(tpeb)2(1,4-ndc)(1,4-Hndc)2]·2.6H2O}n (2), and {[Zn2(tpeb)2(2,3-ndc)2]·H2O}n (3) (tpeb = 1,3,5-tri-4-pyridyl-1,2-ethenylbenzene, 2,5-tdc = 2,5-thiophenedicarboxylic acid, 1,4-ndc = 1,4-naphthalenedicarboxylic acid, and 2,3-ndc = 2,3-naphthalenedicarboxylic acid) were prepared from reactions of Zn(NO3)2·6H2O with tpeb and 2,5-H2tdc, 1,4-H2ndc, or 2,3-H2ndc under solvothermal conditions. Compound 1 has a two-dimensional (2D) grid-like network formed from bridging 1D [Zn(tpeb)]n chains via 2,5-tdc dianions...
March 24, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28339187/accurate-mirna-analysis-in-crude-cell-lysate-by-capillary-electrophoresis-based-hybridization-assay-in-comparison-with-quantitative-reverse-transcription-polymerase-chain-reaction
#6
Liang Hu, Alexander S Stasheuski, David W Wegman, Nan Wu, Burton B Yang, Heyam Hayder, Chun Peng, Stanley K Liu, George M Yousef, Sergey N Krylov
Accurate quantitation of microRNA (miRNA) in tissue samples is required for validation and clinical use of miRNA-based disease biomarkers. Since sample processing, such as RNA extraction, introduces undesirable biases, it is advantageous to measure miRNA in a crude cell lysate. Here we report on accurate miRNA quantitation in crude cell lysate by a CE-based hybridization assay termed direct quantitative analysis of multiple miRNAs (DQAMmiR). Accuracy and precision of miRNA quantitation were determined for miRNA samples in a crude cell lysate, RNA extract from the lysate, and a pure buffer...
March 24, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28338966/a-comparison-of-sensitivity-specificity-imputation-direct-imputation-and-fully-bayesian-analysis-to-adjust-for-exposure-misclassification-when-validation-data-are-unavailable
#7
Marine Corbin, Stephen Haslett, Neil Pearce, Milena Maule, Sander Greenland
Purpose: Measurement error is an important source of bias in epidemiological studies. We illustrate three approaches to sensitivity analysis for the effect of measurement error: imputation of the 'true' exposure based on specifying the sensitivity and specificity of the measured exposure (SS); direct imputation (DI) using a regression model for the predictive values; and adjustment based on a fully Bayesian analysis. Methods: We deliberately misclassify smoking status in data from a case-control study of lung cancer...
March 14, 2017: International Journal of Epidemiology
https://www.readbyqxmd.com/read/28338902/assessment-of-the-status-of-measles-elimination-in-the-united-states-2001-2014
#8
Paul A Gastañaduy, Prabasaj Paul, Amy Parker Fiebelkorn, Susan B Redd, Ben A Lopman, Manoj Gambhir, Gregory S Wallace
We assessed the status of measles elimination in the United States using outbreak notification data. Measles transmissibility was assessed by estimation of the reproduction number, R, the average number of secondary cases per infection, using 4 methods; elimination requires maintaining R at <1. Method 1 estimates R as 1 minus the proportion of cases that are imported. Methods 2 and 3 estimate R by fitting a model of the spread of infection to data on the sizes and generations of chains of transmission, respectively...
March 15, 2017: American Journal of Epidemiology
https://www.readbyqxmd.com/read/28338201/the-clinical-relationship-between-the-slug-mediated-puma-p53-signaling-pathway-and-radiotherapy-resistance-in-nasopharyngeal-carcinoma
#9
T Xu, Y Yuan, D-J Xiao
OBJECTIVE: To explore the clinical relationship between the Slug-mediated Puma/p53 signaling pathway and radiotherapy resistance in nasopharyngeal carcinoma. PATIENTS AND METHODS: Forty surgical specimens were collected from nasopharyngeal carcinoma patients treated at our hospital between February 2010 and February 2015. Twenty patients with poorly differentiated nasopharyngeal carcinoma with and without radiotherapy resistance were included in the experimental and control groups, respectively...
March 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28338199/mir-576-3p-is-a-novel-marker-correlated-with-poor-clinical-outcome-in-bladder-cancer
#10
F-M Meng, F-M Meng, X-L Song
OBJECTIVE: MicroRNAs play important roles in the regulation of the initiation and progression of bladder cancer. To our best knowledge, the prognostic value of miR-576-3p in bladder cancer has not been reported. Thus, the purpose of the current study was to determine the expression of miR-576-3p and assess the clinical significance of miR-576-3p in bladder cancer. PATIENTS AND METHODS: Quantitative real-time polymerase chain reaction (RT-PCR) was performed to evaluate miR-576-3p levels in 232 pairs of bladder cancer specimens and adjacent noncancerous tissues...
March 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28338198/the-expression-of-sirt3-in-primary-hepatocellular-carcinoma-and-the-mechanism-of-its-tumor-suppressing-effects
#11
Y Liu, Y-L Liu, W Cheng, X-M Yin, B Jiang
OBJECTIVE: To observe the SIRT 3 expression in primary hepatocellular carcinoma (HCC), and then establish the eukaryotic expression vector of SIRT3 to observe the proliferation and apoptosis of pZsGreen-c1-SIRT3 HepG2 cells. Furthermore, we explored the mechanism of SIRT3 in inhibiting HCC. PATIENTS AND METHODS: Immunohistochemistry was used to detect the expression of SIRT3 in the tumor tissue and para-tumor tissue in 32 patients with HCC and the normal liver tissue in 10 patients...
March 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28338138/a-novel-colorimetric-assay-for-%C3%AE-methylacyl-coa-racemase-1a-amacr-p504s-utilizing-the-elimination-of-2-4-dinitrophenolate
#12
Maksims Yevglevskis, Guat L Lee, Amit Nathubhai, Yoana D Petrova, Tony D James, Michael D Threadgill, Timothy J Woodman, Matthew D Lloyd
α-Methylacyl-CoA racemase (AMACR; P504S) regulates branched-chain fatty acid degradation, activates Ibuprofen and is a recognised cancer drug target. A novel, facile colorimetric assay was developed based on elimination of 2,4-dinitrophenolate. The assay was used to test 5 known inhibitors, determining IC50 and Ki values, reversibility and characterizing irreversible inhibition.
March 24, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28337476/cerebrospinal-fluid-biomarker-examination-as-a-tool-to-discriminate-behavioral-variant-frontotemporal-dementia-from-primary-psychiatric%C3%A2-disorders
#13
Everard G B Vijverberg, Annemiek Dols, Welmoed A Krudop, Marta Del Campo Milan, Cora J Kerssens, Flora Gossink, Niels D Prins, Max L Stek, Philip Scheltens, Charlotte E Teunissen, Yolande A L Pijnenburg
INTRODUCTION: To prospectively determine the diagnostic value of cerebrospinal fluid (CSF) levels total-tau (tau) to amyloid-β1-42 ratio (Aβ1-42) ratio (tau/Aβ1-42 ratio), phosphorylated-tau (p-tau) to tau ratio (p-tau/tau ratio), neurofilament light chain (NfL) and YKL40 in the late-onset frontal lobe syndrome, in particular for the differential diagnosis of behavioral variant frontotemporal dementia (bvFTD) versus primary psychiatric disorders (PSY). METHOD: We included patients with a multidisciplinary 2-year-follow-up diagnosis of probable/definite bvFTD (n = 22) or PSY (n = 25), who underwent a detailed neuropsychiatric clinical examination, neuropsychological test battery, and magnetic resonance imaging at baseline...
2017: Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring
https://www.readbyqxmd.com/read/28337362/analysis-of-g3bp1-and-vezt-expression-in-gastric-cancer-and-their-possible-correlation-with-tumor-clinicopathological-factors
#14
Mohammadreza Beheshtizadeh, Elham Moslemi
PURPOSE: This study aimed to analyze G3BP1 and VEZT expression profiles in patients with gastric cancer, and examine the possible relationship between the expressions of each gene and clinicopathological factors. MATERIALS AND METHODS: Expression of these genes in formalin-fixed paraffin embedded (FFPE) tissues, collected from 40 patients with gastric cancer and 40 healthy controls, was analyzed. Differences in gene expression among patient and normal samples were identified using the GraphPad Prism 5 software...
March 2017: Journal of Gastric Cancer
https://www.readbyqxmd.com/read/28336434/vav1-expression-is-increased-in-esophageal-squamous-cell-carcinoma-and-indicates-poor-prognosis
#15
Xiaolei Zhu, Huan Jin, Ziwei Xia, Xianxian Wu, Mingjian Yang, Hongdian Zhang, Xiaobin Shang, Runfen Cheng, Zhongli Zhan, Zhentao Yu
Recently, Vav1 has been suggested to play an essential role in the progression of human cancers. However, the correlation between Vav1 expression and prognosis of esophageal squamous cell carcinoma (ESCC) is still unknown. The aim of this study was to investigate Vav1 expression and its prognostic value in ESCC. The expression of Vav1 was detected by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blotting in ESCC tissues and matched nontumorous tissues. Immunohistochemistry (IHC) was carried out to detect Vav1 expression in paraffin samples from 112 primary ESCC patients...
March 20, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28335773/oral-administration-of-propionic-acid-during-lactation-enhances-the-colonic-barrier-function
#16
Zhaobo Xia, Yijiang Han, Ke Wang, Shikun Guo, Dazhou Wu, Xiaozhong Huang, Zhongrong Li, Libin Zhu
BACKGROUND: Propionic acid is a three-carbon short chain fatty acid (SCFA) that has various effects on colonic functions. Although several studies have shown the effects of propionic acid on intestinal mucosal barrier function, studies of the promotion effect during pre-weaning are rare in the literature as far as we know. METHODS: Pre-weaning male Sprague-Dawley rats 7 days after birth were given an oral 0.2 mL/10 g of 200 mM propionic acid solution in the propionic acid group or normal saline solution in the control group by gavage twice a day for ten days...
March 23, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28335103/correlation-between-gastric-emptying-and-gastric-adaptive-relaxation-influenced-by-amino-acids
#17
Masayuki Uchida, Orie Kobayashi, Chizuru Saito
Background/Aims: Amino acids have many physiological activities. We report the correlation between gastric emptying and gastric adaptive relaxation using tryptophan and amino acids with a straight alkyl chain, hydroxylated chain, and branched chain. Here we sought to further clarify the correlation between gastric emptying and gastric adaptive relaxation by using other amino acids. Methods: In Sprague-Dawley rats, gastric emptying was evaluated by a breath test using [1-¹³C] acetic acid...
March 23, 2017: Journal of Neurogastroenterology and Motility
https://www.readbyqxmd.com/read/28334647/arsenic-load-in-rice-ecosystem-and-its-mitigation-through-deficit-irrigation
#18
Arkabanee Mukherjee, M Kundu, B Basu, B Sinha, M Chatterjee, M Das Bairagya, U K Singh, S Sarkar
Rice the staple food is a notable intake source of arsenic to the rural population of eastern India through food-chain. A field survey was carried out to study the variation of arsenic load in different parts of rice genotype Shatabdi (most popular genotype of the region) exposed to varying level of arsenic present in the irrigation water and soil. As irrigation is the primary source of arsenic contamination, a study was conducted to assess arsenic load in rice ecosystem under deficit irrigation practices like intermittent ponding (IP), saturation (SAT) and aerobic (AER) imposed during stress allowable stage (16-40 days after transplanting) of the crop (genotype Shatabdi)...
March 20, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28333931/differential-gene-expression-in-patients-with-subsyndromal-symptomatic-depression-and-major-depressive-disorder
#19
Chengqing Yang, Guoqin Hu, Zezhi Li, Qingzhong Wang, Xuemei Wang, Chengmei Yuan, Zuowei Wang, Wu Hong, Weihong Lu, Lan Cao, Jun Chen, Yong Wang, Shunying Yu, Yimin Zhou, Zhenghui Yi, Yiru Fang
BACKGROUND: Subsyndromal symptomatic depression (SSD) is a subtype of subthreshold depressive and can lead to significant psychosocial functional impairment. Although the pathogenesis of major depressive disorder (MDD) and SSD still remains poorly understood, a set of studies have found that many same genetic factors play important roles in the etiology of these two disorders. Nowadays, the differential gene expression between MDD and SSD is still unknown. In our previous study, we compared the expression profile and made the classification with the leukocytes by using whole-genome cRNA microarrays among drug-free first-episode subjects with SSD, MDD and matched healthy controls (8 subjects in each group), and finally determined 48 gene expression signatures...
2017: PloS One
https://www.readbyqxmd.com/read/28333167/a-complex-intragenic-rearrangement-of-ercc8-in-chinese-siblings-with-cockayne-syndrome
#20
Hua Xie, Xiaoyan Li, Jiping Peng, Qian Chen, ZhiJie Gao, Xiaozhen Song, WeiYu Li, Jianqiu Xiao, Caihua Li, Ting Zhang, James F Gusella, Jianmin Zhong, Xiaoli Chen
Cockayne syndrome is an autosomal recessive disorder principally characterized by postnatal growth failure and progressive neurological dysfunction, due primarily to mutations in ERCC6 and ERCC8. Here, we report our diagnostic experience for two patients in a Chinese family suspected on clinical grounds to have Cockayne syndrome. Using multiple molecular techniques, including whole exome sequencing, array comparative genomic hybridization and quantitative polymerase chain reaction, we identified compound heterozygosity for a maternal splicing variant (chr5:60195556, NM_000082:c...
March 23, 2017: Scientific Reports
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