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https://www.readbyqxmd.com/read/28108411/production-and-characterization-of-medium-chain-length-polyhydroxyalkanoates-copolymer-from-arctic-psychrotrophic-bacterium-pseudomonas-sp-pamc-28620
#1
Ganesan Sathiyanarayanan, Shashi Kant Bhatia, Hun-Suk Song, Jong-Min Jeon, Jun-Young Kim, Yoo Kyung Lee, Yun-Gon Kim, Yung-Hun Yang
Arctic psychrotrophic bacterium Pseudomonas sp. PAMC 28620 was found to produce a distinctive medium-chain-length polyhydroxyalkanoates (MCL-PHAs) copolymer when grown on structurally unrelated carbon sources including glycerol. The maximum MCL-PHAs copolymer yield was obtained about 52.18±4.12% from 7.95±0.66g/L of biomass at 144h of fermentation when 3% glycerol was used as sole carbon and energy source during the laboratory-scale bioreactor process. Characterization of the copolymer was carried out using fourier transform infrared spectroscopy (FTIR), gas chromatography-mass spectrometry (GC-MS), proton ((1)H) and carbon ((13)C) nuclear magnetic resonance spectroscopy (NMR), gel permeation chromatography (GPC), differential scanning calorimeter (DSC) and thermo-gravimetric analysis (TGA)...
January 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28108002/reduced-order-model-based-active-disturbance-rejection-control-of-hydraulic-servo-system-with-singular-value-perturbation-theory
#2
Chengwen Wang, Long Quan, Shijie Zhang, Hongjun Meng, Yuan Lan
Hydraulic servomechanism is the typical mechanical/hydraulic double-dynamics coupling system with the high stiffness control and mismatched uncertainties input problems, which hinder direct applications of many advanced control approaches in the hydraulic servo fields. In this paper, by introducing the singular value perturbation theory, the original double-dynamics coupling model of the hydraulic servomechanism was reduced to a integral chain system. So that, the popular ADRC (active disturbance rejection control) technology could be directly applied to the reduced system...
January 17, 2017: ISA Transactions
https://www.readbyqxmd.com/read/28106723/inhibition-of-autophagic-degradation-process-contributes-to-claudin-2-expression-increase-and-epithelial-tight-junction-dysfunction-in-tnf-%C3%AE-treated-cell-monolayers
#3
Cong Zhang, Junkai Yan, Yongtao Xiao, Yujie Shen, Jiazheng Wang, Wensong Ge, Yingwei Chen
Tight junction dysfunction plays a vital role in some chronic inflammatory diseases. Pro-inflammatory cytokines, especially tumor necrosis factor alpha (TNF-α), act as important factors in intestinal epithelial tight junction dysfunction during inflammatory conditions. Autophagy has also been shown to be crucial in tight junction function and claudin-2 expression, but whether autophagy has an effect on the change of claudin-2 expression and tight junction function induced by TNF-α is still unknown. To answer this question, we examined the expression of claudin-2 protein, transepithelial electrical resistance (TER), and permeability of cell monolayers, autophagy flux change, and lysosomal pH after TNF-α with or without PP242 treatment...
January 17, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28106228/expression-of-long-non-coding-rna-pandar-and-its-prognostic-value-in-colorectal-cancer-patients
#4
Xiangke Li, Feng Wang, Yan Sun, Qingxia Fan, Guangfei Cui
BACKGROUND: Long noncoding RNAs (lncRNAs) are emerging as key molecules in human cancer. In the present study, we explored the role of the lncRNA PANDAR in colorectal cancer (CRC). METHODS: The relative expression level of lncRNA PANDAR in CRC tissues and cell lines was determined by quantitative real-time polymerase chain reaction (qRT-PCR). The associations between PANDAR expression and clinicopathological features of CRC patients were further analyzed. Kaplan-Meier survival analysis was performed to evaluate the value of PANDAR in the prognosis of CRC patients...
January 20, 2017: International Journal of Biological Markers
https://www.readbyqxmd.com/read/28106221/the-magnetoresistance-effect-in-a-conducting-molecular-crystal-consisting-of-dicyano-phthalocyaninato-manganese-iii
#5
Masaki Matsuda, Gosuke Yoshida, Jun-Ichi Yamaura, Tamotsu Inabe, Hiroyuki Tajima
A conducting molecular crystal TPP[Mn(III)(Pc)(CN)2]2 (Mn(III): d(4), S = 1, TPP = tetraphenylphosphonium and Pc = phthalocyanine) was fabricated. In its crystal structure, the [Mn(III)(Pc)(CN)2] units formed a one-dimensional regular chain along the c-axis with an overlap integral value of 8.6 × 10(-3). TPP[Mn(III)(Pc)(CN)2]2 showed a semiconducting behaviour that also has been observed for isostructural TPP[Co(III)(Pc)(CN)2]2 (Co(III): d(6), S = 0) and TPP[Fe(III)(Pc)(CN)2]2 (Fe(III): d(5), S = 1/2) whose ground states are charge-ordered states...
January 20, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28105565/what-are-the-socioeconomic-implications-of-the-value-chain-of-biodiversity-products-a-case-study-in-northeastern-brazil
#6
Rafael Ricardo Vasconcelos da Silva, Laura Jane Gomes, Ulysses Paulino Albuquerque
The trade in biodiversity products has gained increasing importance in conservation and livelihood strategies. The aim of this study is to describe and analyze the socioeconomic aspects and dynamics of the production and commercialization of two important products of Brazilian biodiversity from the fruits of Caryocar coriaceum Wittm. and Dimorphandra gardneriana Tul. Interviews were conducted with who were involved in the various stages of value chains. Data were analyzed under an analytical/descriptive approach...
February 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28105353/isolated-extramedullary-cutaneous-relapse-despite-concomitant-severe-graft-vs-host-disease-and-tissue-chimerism-analysis-in-a-patient-with-acute-lymphoblastic-leukemia-after-allogeneic-hematopoietic-stem-cell-transplantation-a-case-report
#7
Bulent Kantarcioglu, Huseyin Saffet Bekoz, Yeliz Duvarci Ogret, Asli Cakir, Demet Kivanc, Fatma Savran Oguz, Deniz Sargin
Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is a potentially curative treatment option for patients with acute lymphoblastic leukemia (ALL). The curative potential of allo-HSCT for ALL is, in part, due to the graft-vs.-leukemia (GVL) effect, in addition to the intensive conditioning chemo-radiotherapy. However, relapse remains the major cause of treatment failure following allo-HSCT for ALL. In the allo-HSCT setting, testing for genetic markers of hematopoietic chimerism has become a part of the routine diagnostic program...
December 2016: Molecular and Clinical Oncology
https://www.readbyqxmd.com/read/28105215/role-of-axl-expression-in-non-small-cell-lung-cancer
#8
Xiaohan Qu, Jinlu Liu, Xinwen Zhong, Xi Li, Qigang Zhang
The present study aimed to investigate the expression profile of AXL in non-small cell lung cancer (NSCLC) and its clinical significance. The current study included 257 NSCLC patients, tyrosine-protein kinase receptor UFO (AXL) expression in paired lung cancer and adjacent normal lung tissues of NSCLC patients were compared by immunohistochemistry, western blot analysis and quantitative polymerase chain reaction (qPCR). These methods were used to detect the expression of the AXL gene and protein in fresh tissues from 35 patients...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28105191/plasma-lncrna-gacat2-is-a-valuable-marker-for-the-screening-of-gastric-cancer
#9
Lin Tan, Yunben Yang, Yongfu Shao, Haiqiang Zhang, Junming Guo
Long non-coding RNAs (lncRNAs) are crucial in contributing to gastric tumorigenesis and development. However, the diagnostic value of the majority of lncRNAs in gastric cancer (GC) are not clear. The present study investigated the diagnostic value of gastric cancer associated transcript 2 (GACAT2), a lncRNA that is aberrantly expressed in GC tissues. A total of 343 plasma samples from 80 healthy individuals, 29 patients with gastric dysplasia (GD) and 117 paired preoperative and postoperative patients with GC were collected...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28105190/prognostic-significance-of-akr1b10-gene-expression-in-hepatocellular-carcinoma-and-surrounding-non-tumorous-liver-tissue
#10
Fuminori Sonohara, Yoshikuni Inokawa, Mitsuhiro Hishida, Mitsuro Kanda, Yoko Nishikawa, Suguru Yamada, Tsutomu Fujii, Hiroyuki Sugimoto, Yasuhiro Kodera, Shuji Nomoto
When assessing outcome in hepatocellular carcinoma (HCC), it is important to consider prognostic factors in background non-tumorous liver tissue as well as in the tumor, since multiple occurrence is associated with background liver status such as hepatitis. The current study aimed to elucidate molecular prognostic predictors that have an association with HCC background non-tumorous tissue. Microarray expression profiling identified aldo-keto reductase family 1, member B10 (AKR1B10) as a putative non-tumorous prognostic factor, and AKR1B10 gene expression was investigated in 158 curatively resected HCC cases by reverse transcription-quantitative polymerase chain reaction...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28105140/role-of-mir-145-in-chronic-constriction-injury-in-rats
#11
Xiaolin Pang, Yuanzhang Tang, Dongya Zhang
The present study aims to investigate the effects and underlying mechanisms of miRNA-145 (miR-145) in rat models of chronic constriction injury (CCI). Rats were randomly divided into control, sham, CCI, agomiRNA (agomiR)-normal control (NC) and agomiR-145 groups (n=25 in each group); in addition, 30 rats with CCI were divided into small hairpin (sh)RNA-NC and shRNA-ras responsive element binding protein 1 (RREB1) groups. Paw withdrawal thermal latency (PWTL) and paw withdrawal mechanical threshold (PWMT) were detected...
December 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28104409/gigantol-from-dendrobium-chrysotoxum-lindl-binds-and-inhibits-aldose-reductase-gene-to-exert-its-anti-cataract-activity-an-in-vitro-mechanistic-study
#12
Jie Wu, Xue Li, Wencheng Wan, Qiaohong Yang, Weifeng Ma, Dan Chen, Jiangmiao Hu, C-Y Oliver Chen, Xiaoyong Wei
ETHNOPHARMACOLOGICAL RELEVANCE: Dendrobium. chrysotoxum Lindl is a commonly used species of medicinal Dendrobium which belongs to the family of Orchidaceae, locally known as "Shihu" or "Huangcao". D. chrysotoxum Lindl is widely known for medicinal values in traditional Chinese medicine as it possesses anti-inflammatory, anti-hyperglycemic induction, antitumor and antioxidant properties. STUDY AIM: To characterize the interaction between gigantol extracted from D...
January 16, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28103883/performance-of-loop-mediated-isothermal-amplification-lamp-for-the-diagnosis-of-malaria-among-malaria-suspected-pregnant-women-in-northwest-ethiopia
#13
Banchamlak Tegegne, Sisay Getie, Wossenseged Lemma, Abu Naser Mohon, Dylan R Pillai
BACKGROUND: Malaria is a major public health problem and an important cause of maternal and infant morbidity in sub-Saharan Africa, including Ethiopia. Early and accurate diagnosis of malaria with effective treatment is the best strategy for prevention and control of complications during pregnancy and infant morbidity and mortality. However, laboratory diagnosis has relied on the identification of malaria parasites and parasite antigens in peripheral blood using Giemsa-stained microscopy or rapid diagnostic tests (RDTs) which lack analytical and clinical sensitivity...
January 19, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28103395/influence-of-lipid-content-in-a-corn-oil-preparation-on-the-bioaccessibility-of-%C3%AE-carotene-a-comparison-of-low-fat-and-high-fat-samples
#14
Ziyuan Xia, David Julian McClements, Hang Xiao
Some individuals with fat maldigestion have compromised digestive systems, which causes the incomplete hydrolyzation of ingested lipids within the gastrointestinal tract (GIT). We studied the influence of high-fat (20%) and low-fat (4%) contents on the bioaccessibility of a highly hydrophobic nutraceutical (β-carotene) through a simulated GIT model consisting of mouth, stomach, and small intestine phases. The low-fat and high-fat values were chosen to simulate low-fat and high-fat diets. The triglycerides in the low-fat system were fully digested, whereas those in the high-fat system were only partially digested, thereby mimicking the digestive systems of individuals who exhibit fat maldigestion...
January 19, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28103296/production-of-fatty-acids-and-protein-by-nannochloropsis-in-flat-plate-photobioreactors
#15
Chris J Hulatt, René H Wijffels, Sylvie Bolla, Viswanath Kiron
Nannochloropsis is an industrially-promising microalga that may be cultivated for alternative sources of nutrition due to its high productivity, protein content and lipid composition. We studied the growth and biochemical profile of Nannochloropsis 211/78 (CCAP) in optimized flat-plate photobioreactors. Eighteen cultivations were performed at two nutrient concentrations. The fatty acid, protein content and calorific values were analyzed after 8, 12 and 16 days. Neutral lipids were separated and the changes in fatty acids in triglycerides (TAGs) during nutrient depletion were recorded...
2017: PloS One
https://www.readbyqxmd.com/read/28101948/influence-of-the-marine-feeding-area-on-the-muscle-and-egg-fatty-acid-composition-of-atlantic-salmon-salmo-salar-spawners-estimated-from-the-scale-stable-isotopes
#16
J Torniainen, M J Kainz, R I Jones, M Keinänen, P J Vuorinen, M Kiljunen
Fatty acids in muscle tissue and eggs of female Atlantic salmon Salmo salar spawners were analysed to evaluate the dietary quality of their final feeding areas in the Baltic Sea. The final likely feeding area was identified by comparing stable carbon and nitrogen isotope composition of the outermost growth region (final annulus) of scales of returned S. salar with that of reference S. salar caught from different feeding areas. Some overlap of stable-isotope reference values among the three areas, in addition to prespawning fasting, decreased the ability of muscle tri-acylglycerols to discriminate the final likely feeding area and the area's dietary quality...
January 18, 2017: Journal of Fish Biology
https://www.readbyqxmd.com/read/28101518/is-it-time-to-include-ct-reverse-halo-sign-and-qpcr-targeting-mucorales-in-serum-to-eortc-msg-criteria-for-the-diagnosis-of-pulmonary-mucormycosis-in-leukemia-patients
#17
Denis Caillot, Stéphane Valot, Ingrid Lafon, Louise Basmaciyan, Marie Lorraine Chretien, Marc Sautour, Laurence Million, Caroline Legouge, Alexandre Payssot, Frédéric Dalle
In 23 leukemia patients with proven (n = 17) or possible (n = 6) pulmonary mucormycosis (PM), the presence of reversed halo sign on computed tomography was strongly associated with the positivity of quantitative polymerase chain reaction assays targeting Mucorales in the serum, confirming the value of these two tools for the diagnosis of PM in this setting.
October 2016: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/28101227/downregulation-of-mir-23b-in-plasma-is-associated-with-poor-prognosis-in-patients-with-colorectal-cancer
#18
Chang-Hua Kou, Tian Zhou, Xi-Lin Han, Hui-Jie Zhuang, Hai-Xin Qian
MicroRNAs (miRNAs) are short, non-coding RNA molecules that act as regulators of gene expression. Circulating blood miRNAs have potential as cancer biomarkers. The main objective of the present study was to assess the effect of miRNA-23b (miR-23b) expression in plasma on the diagnosis and prognosis of colorectal cancer (CRC). Reverse transcription-quantitative polymerase chain reaction (PCR) was used to measure miR-23b expression levels, and methylation-specific PCR was used to test the promoter methylation status...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28101226/evaluation-of-plasma-microrna-levels-to-predict-insensitivity-of-patients-with-advanced-lung-adenocarcinomas-to-pemetrexed-and-platinum
#19
Jinghua Zhu, Yuhua Qi, Jianzhong Wu, Meiqi Shi, Jifeng Feng, Longbang Chen
Pemetrexed combined with platinum is a first-line therapy used to treat patients with advanced non-small cell lung cancer (NSCLC) that exhibit negative or unknown epidermal growth factor receptor (EGFR) mutational status or anaplastic lymphoma kinase (ALK) rearrangements. Lung adenocarcinoma (LAC) is the primary type of NSCLC. In order to prevent overtreatment, it is necessary to identify patients with LAC who may not benefit from certain chemotherapies. Patients recruited in the present study (n=129) were diagnosed with advanced LAC and received first-line pemetrexed and platinum-based chemotherapy...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28099753/correlation-between-rapid-hiv-testing-and-fourth-generation-elisa-results-for-hiv-detection-among-pregnant-patients-in-the-delivery-room
#20
Alejandro G Almaguer, Lidia Mendoza-Flores, Luis A Sánchez-López, Laura A Palau-Dávila, Magaly Padilla-Orozco, Adrián Camacho-Ortiz
OBJECTIVE: To analyze the usefulness of rapid HIV testing in pregnant patients in the delivery room. METHODS: This prospective study compared a rapid test and a fourth-generation enzyme-linked immunoassay (ELISA) for HIV screening among pregnant patients admitted in labor with an unknown HIV status at a university hospital in Mexico between July 2015 and February 2016. Pearson correlation analysis was performed, and the diagnostic accuracy of the two tests was assessed with HIV RNA polymerase chain reaction (PCR) as the reference method...
January 5, 2017: International Journal of Gynaecology and Obstetrics
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