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https://www.readbyqxmd.com/read/29785536/wnt3a-promotes-differentiation-of-human-bone-marrow-derived-mesenchymal-stem-cells-into-cementoblast-like-cells
#1
Yusuke Aida, Hidemi Kurihara, Koichi Kato
Cementum is a calcified, avascular connective tissue that laminates the root of a tooth and plays a pivotal role in the development, homeostasis, and regeneration of a periodontal tissue. As a potential treatment for periodontal tissue defects in the patient with chronic periodontitis, much attention has been paid to tissue engineering combined with mesenchymal stem cells for regenerating periodontal tissues including cementum. However, limited information is available for the molecular factors that have impacts on the differentiation of mesenchymal stem cells into cementoblasts...
May 21, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29780246/remineralization-potential-of-nano-hydroxyapatite-on-enamel-and-cementum-surrounding-margin-of-computer-aided-design-and-computer-aided-manufacturing-ceramic-restoration
#2
Niwut Juntavee, Apa Juntavee, Preeyarat Plongniras
Objective: This study investigates the effects of nano-hydroxyapatite (NHA) gel and Clinpro (CP) on remineralization potential of enamel and cementum at the cavosurface area of computer-aided design and computer-aided manufacturing ceramic restoration. Materials and methods: Thirty extracted human mandibular third molars were sectioned at 1 mm above and below the cemento-enamel junction to remove the cemento-enamel junction portions and replaced them with zirconia ceramic disks by bonding them to the crown and root portions with resin cement...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29779281/-effects-of-tumor-necrosis-factor-%C3%AE-on-osteogenic-differentiation-and-notch-signaling-pathway-in-human-periodontal-ligament-stem-cells
#3
Yu Ma, Shu-Hui Li, Xin-Xin Ding, Pei-Ling Wu
OBJECTIVE: To evaluate the effects of tumor necrosis factor-α (TNF-α) on osteogenic differentiation and Notch signaling pathway of periodontal ligament stem cells (PDLSCs) and to investigate the regulatory role of Notch signaling pathway on the osteogenic differentiation of PDLSCs under the influence of TNF-α. METHODS: PDLSCs were obtained through enzyme digestion and tissue block method. The expression levels of stem cell surface markers CD105, CD90, CD146, CD45, and CD31 were detected by fluorescence activated cell sorter (FACS)...
April 1, 2018: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/29765167/the-mineral-content-of-the-hard-dental-tissue-of-mesiodens
#4
Sanja Tanaskovic-Stankovic, Irena Tanaskovic, Nemanja Jovicic, Marina Miletic-Kovacevic, Tatjana Kanjevac, Zoran Milosavljevic
OBJECTIVE: Mesiodens is the most common form of supernumerary tooth mainly located between the maxillary central incisors. Its etiology is not completely understood but both genetic and environmental factors are assumed. The degree of mineralization and inorganic element content in hard tooth tissues is poorly understood as well as is the durability and suitability for allo- and auto-transplantation. Therefore aim of this study was to examine the content of inorganic elements. MATERIALS AND METHODS: This study included 26 mesiodens teeth and 26 normal central incisor teeth as controls...
May 15, 2018: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/29756133/biology-of-teeth-and-implants-host-factors-pathology-regeneration-and-the-role-of-stem-cells
#5
F-Michael Eggert, Liran Levin
In chronic periodontitis and peri-implantitis, cells of the innate and adaptive immune systems are involved directly in the lesions within the tissues of the patient. Absence of a periodontal ligament around implants does not prevent a biologic process similar to that of periodontitis from affecting osseointegration. Our first focus is on factors in the biology of individuals that are responsible for the susceptibility of such individuals to chronic periodontitis and to peri-implantitis. Genetic factors are of significant importance in susceptibility to these diseases...
2018: Quintessence International
https://www.readbyqxmd.com/read/29753151/isolation-of-dna-from-small-amounts-of-elephant-ivory-sampling-the-cementum-with-total-demineralization-extraction
#6
M Winters, A Torkelson, R Booth, C Mailand, Y Hoareau, S Tucker, S K Wasser
Genotyping ivory samples can determine the geographic origin of poached ivory as well as the legality of ivory being sold in ivory markets. We conducted a series of experiments to determine where the DNA is most concentrated in ivory samples and how best to increase DNA yield from groups of samples likely to vary in DNA concentration. We examined variation in DNA amplification success from: the layer(s) of the tusk (cementum and/or dentine) being extracted, demineralization temperature and time, and the concentration of eluates...
April 27, 2018: Forensic Science International
https://www.readbyqxmd.com/read/29748557/recombinant-amelogenin-regulates-the-bioactivity-of-mouse-cementoblasts-in-vitro
#7
Sema S Hakki, S Buket Bozkurt, Emre Türkay, Michel Dard, Nuhan Purali, Werner Götz
Amelogenin (AMG) is a cell adhesion molecule that has an important role in the mineralization of enamel and regulates events during dental development and root formation. The purpose of the present study was to investigate the effects of recombinant human AMG (rhAMG) on mineralized tissue-associated genes in cementoblasts. Immortalized mouse cementoblasts (OCCM-30) were treated with different concentrations (0.1, 1, 10, 100, 1000, 10,000, 100,000 ng · mL-1 ) of recombinant human AMG (rhAMG) and analyzed for proliferation, mineralization and mRNA expression of bone sialoprotein (BSP), osteocalcin (OCN), collagen type I (COL I), osteopontin (OPN), runt-related transcription factor 2 (Runx2), cementum attachment protein (CAP), and alkaline phosphatase (ALP) genes using quantitative RT-PCR...
May 9, 2018: International Journal of Oral Science
https://www.readbyqxmd.com/read/29745817/impaired-mineral-quality-in-dentin-in-x-linked-hypophosphatemia
#8
Benjamin R Coyac, Guillaume Falgayrac, Guillaume Penel, Alain Schmitt, Thorsten Schinke, Agnès Linglart, Marc D McKee, Catherine Chaussain, Claire Bardet
X-linked hypophosphatemia (XLH) is a skeletal disorder arising from mutations in the PHEX gene, transmitted in most cases as an X-linked dominant trait. PHEX deficiency leads to renal phosphate wasting and hypophosphatemia, as well as impaired mineralization of bone and dentin, resulting in severe skeletal and dental complications. Dentin mineralization defects appear as characteristic, large interglobular spaces resulting from the lack of fusion of calculospherites in the circumpulpal region during the mineralization process...
December 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29731944/exploring-the-gingival-recession-surgical-treatment-modalities-a-literature-review
#9
REVIEW
Mirsad Shkreta, Aneta Atanasovska-Stojanovska, Blerta Dollaku, Zlatanka Belazelkoska
Gingival recessions present complex soft tissue pathology, with a multiple aetiology and a high prevalence which increases with age. They are defined as an exposure of the root surface of the teeth as a result of the apical migration of the gingival margin beyond the cementum-enamel junction, causing functional and aesthetic disturbances to the affected individuals. Aiming to ensure complete root coverage and satisfying aesthetic outcomes, a wide range of surgical techniques have been proposed through the decades for the treatment of the gingival recessions...
April 15, 2018: Open Access Macedonian Journal of Medical Sciences
https://www.readbyqxmd.com/read/29731572/quantitative-analysis-of-dental-age-estimation-by-incremental-line-of-cementum
#10
G Swetha, Kiran Kumar Kattappagari, Chandra Shekar Poosarla, Lalith Prakash Chandra, Sridhar Reddy Gontu, Venkata Ramana Reddy Badam
Aim: This study aims to examine the correlation between age and number of incremental lines in human dental cementum among single-rooted teeth (incisors and canines) and to assess the best tooth to estimate age group by studying cementum under phase-contrast microscope and to assess the use of cementum in age estimation. Materials and Methods: The present study was carried out in the Department of Oral Pathology and Microbiology. A sample of eighty single-rooted undecalcified longitudinal ground sections is prepared from freshly collected teeth with age noted separately and observed under phase-contrast microscope, photographed and counted...
January 2018: Journal of Oral and Maxillofacial Pathology: JOMFP
https://www.readbyqxmd.com/read/29714697/evaluation-of-the-marginal-integrity-of-a-bioactive-restorative-material
#11
Barry M Owens, Jeffrey G Phebus, William W Johnson
This in vitro study evaluated the marginal microleakage of a bioactive restorative with other restorative materials in standard Class V preparations. Sixty previously extracted, noncarious human molars were randomly assigned to 3 experimental groups (n = 20): a bioactive composite resin, a universal hybrid composite resin, and a resin-modified glass ionomer restorative. Class V cavities were prepared on the facial or lingual surface of each tooth so that coronal margins were located in enamel and apical margins in cementum (dentin)...
May 2018: General Dentistry
https://www.readbyqxmd.com/read/29713351/the-role-of-fibroblast-growth-factors-in-tooth-development-and-incisor-renewal
#12
REVIEW
Wen Du, Wei Du, Haiyang Yu
The mineralized tissue of the tooth is composed of enamel, dentin, cementum, and alveolar bone; enamel is a calcified tissue with no living cells that originates from oral ectoderm, while the three other tissues derive from the cranial neural crest. The fibroblast growth factors (FGFs) are critical during the tooth development. Accumulating evidence has shown that the formation of dental tissues, that is, enamel, dentin, and supporting alveolar bone, as well as the development and homeostasis of the stem cells in the continuously growing mouse incisor is mediated by multiple FGF family members...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29713167/characterization-and-evaluation-of-graphene-oxide-scaffold-for-periodontal-wound-healing-of-class-ii-furcation-defects-in-dog
#13
Kohei Kawamoto, Hirofumi Miyaji, Erika Nishida, Saori Miyata, Akihito Kato, Akito Tateyama, Tomokazu Furihata, Kanako Shitomi, Toshihiko Iwanaga, Tsutomu Sugaya
Introduction: The 3-dimensional scaffold plays a key role in volume and quality of repair tissue in periodontal tissue engineering therapy. We fabricated a novel 3D collagen scaffold containing carbon-based 2-dimensional layered material, named graphene oxide (GO). The aim of this study was to characterize and assess GO scaffold for periodontal tissue healing of class II furcation defects in dog. Materials and methods: GO scaffolds were prepared by coating the surface of a 3D collagen sponge scaffold with GO dispersion...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29704248/cementum-protein-1-transfection-does-not-lead-to-ultrastructural-changes-in-nucleolar-organization-of-human-gingival-fibroblasts
#14
C E Villegas-Mercado, L T Agredano-Moreno, M Bermúdez, M L Segura-Valdez, H Arzate, E F Del Toro-Rangel, L F Jiménez-García
BACKGROUND AND OBJECTIVE: Transfection of cementum protein 1 (CEMP1) into human gingival fibroblasts (HGFs) notably increases cell metabolism and results in overexpression of molecules related to biomineralization at transcriptional and protein levels. Therefore, HGF-CEMP1 cells are considered as putative cementoblasts. This represents a significant advance in periodontal research because cementum neoformation is a key event in periodontal regeneration. In addition, it is well known that important changes in cell metabolism and protein expression are related to nucleolar structure and the function of this organelle, which is implicated in ribosome biogenesis...
April 27, 2018: Journal of Periodontal Research
https://www.readbyqxmd.com/read/29704238/microrna-3064-3p-regulates-the-differentiation-of-cementoblasts-through-targeting-dkk1
#15
C Wang, H Liao, H Sun, Y Zhang, Z Cao
BACKGROUND AND OBJECTIVE: MicroRNAs (miRNAs) are short, noncoding RNAs that interfere with translation of target mRNAs and thereby play a pivotal role in a variety of biological processes. Cementoblasts are the cells that build up cementum. They share a similar gene expression pattern with osteoblasts. Recent studies have suggested that miRNAs are able to control osteoblast-mediated bone formation. However, the effects of miRNA on cementoblast differentiation still remain unsolved. Herein, we wanted to elucidate the role of miR-3064-3p in cementoblast differentiation...
April 27, 2018: Journal of Periodontal Research
https://www.readbyqxmd.com/read/29698557/a-simplified-silver-phosphate-extraction-method-for-oxygen-isotope-analysis-of-bioapatite
#16
Brandi M Shabaga, Hilary Gough, Mostafa Fayek, Robert D Hoppa
RATIONALE: Although phosphatic materials are chemically complex and are prone to exchange oxygen isotopes with their environments, the phosphate (PO4 3- ) component of these materials is robust and retains its original oxygen isotopic composition. As a result, there are currently several methods for the isolation of phosphate oxygen through the precipitation of silver phosphate (Ag3 PO4 ). However, some of these techniques produce Ag3 PO4 of questionable purity, while nearly all are lengthy and/or require relatively large sample sizes...
April 26, 2018: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29695816/comparative-study-on-differentiation-of-cervical-loop-cells-and-hertwig-s-epithelial-root-sheath-cells-under-the-induction-of-dental-follicle-cells-in-rat
#17
Yongwen Guo, Weihua Guo, Jie Chen, Ye Tian, Guoqing Chen, Weidong Tian, Ding Bai
Cervical loop cells (CLC) and Hertwig's epithelial root sheath (HERS) cells are believed to play critical roles in distinct developmental patterns between rodent incisors and molars, respectively. However, the differences in differentiation between CLC and HERS cells, and their response to inductions from dental follicle cells, remain largely unknown. In present study, CLC and HERS cells, as well as incisor dental follicle (IF) cells and molar dental follicle (MF) cells were isolated from post-natal 7-day rats...
April 25, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29695118/effect-of-amelogenin-coating-of-a-nano-modified-titanium-surface-on-bioactivity
#18
Chisato Terada, Satoshi Komasa, Tetsuji Kusumoto, Takayoshi Kawazoe, Joji Okazaki
The interactions between implants and host tissues depend on several factors. In particular, a growing body of evidence has demonstrated that the surface texture of an implant influences the response of the surrounding cells. The purpose of this study is to develop new implant materials aiming at the regeneration of periodontal tissues as well as hard tissues by coating nano-modified titanium with amelogenin, which is one of the main proteins contained in Emdogain® . We confirmed by quartz crystal microbalance evaluation that amelogenin is easy to adsorb onto the nano-modified titanium surface as a coating...
April 24, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29694875/influence-of-micropatterning-on-human-periodontal-ligament-cells-behavior
#19
Lisha Zheng, Jingyi Jiang, Jinpeng Gui, Lingyu Zhang, Xiaoyi Liu, Yan Sun, Yubo Fan
The periodontal ligament (PDL) is highly ordered connective tissue located between the alveolar bone and cementum. An aligned and organized architecture is required for its physiological function. We applied micropatterning technology to arrange PDL cells in 10- or 20-μm-wide extracellular protein patterns. Cell and nuclear morphology, cytoskeleton, proliferation, differentiation, and matrix metalloproteinase system expression were investigated. Micropatterning clearly elongated PDL cells with a low cell-shape index and low spreading area...
April 24, 2018: Biophysical Journal
https://www.readbyqxmd.com/read/29692662/changes-in-the-chemical-composition-of-mineralised-teeth-in-children-after-antineoplastic-treatment
#20
Ewa Krasuska-Sławińska, Bożenna Dembowska-Bagińska, Agnieszka Brożyna, Dorota Olczak-Kowalczyk, Elżbieta Czarnowska, Agnieszka Sowińska
Introduction: Chemotherapy, neoplasms, and their complications linked to malabsorption, malnutrition, and metabolic disorders may lead to improper tooth development and frequent severe caries in patients during/after antineoplastic treatment and to a more frequent improper tooth development in patients undergoing chemotherapy during odontogenesis. However, the causes of these abnormalities remain unknown; there are no studies on the impact of antineoplastic treatment and its complications on the chemical composition of mineralised teeth...
2018: Contemporary Oncology Współczesna Onkologia
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