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https://www.readbyqxmd.com/read/29333321/periodontal-wound-healing-following-reciprocal-autologous-root-transplantation-in-class-iii-furcation-defects
#1
Naoshi Takeuchi, Yoshinori Shirakata, Yukiya Shinohara, Kotaro Sena, Kazuyuki Noguchi
Purpose: Furcation involvement in the molars is difficult to treat, and has been recognized as a risk factor for tooth loss. Although periodontal regenerative therapies, including guided tissue regeneration and various types of bone grafts, have been applied to furcation defects, the effects of these treatments are limited, especially in large class III furcation defects. The purpose of this pilot study was to investigate the effect of reciprocal autologous root transplantation on periodontal wound healing and regeneration in class III furcation defects in dogs...
December 2017: Journal of Periodontal & Implant Science
https://www.readbyqxmd.com/read/29328966/cementum-as-a-source-of-dna-in-challenging-forensic-cases
#2
Hussam Mansour, Oliver Krebs, Jan Peter Sperhake, Christa Augustin, Till Koehne, Michael Amling, Klaus Püschel
Each forensic case is characterized by its own uniqueness. Deficient forensic cases require additional sources of human identifiers to assure the identity. We report on two different cases illustrating the role of teeth in answering challenging forensic questions. The first case involves identification of an adipocere male found in a car submersed in water for approximately 2 years. The second scenario, which involves paternity DNA testing of an exhumed body, was performed approximately 2.8 years post-mortem...
January 3, 2018: Journal of Forensic and Legal Medicine
https://www.readbyqxmd.com/read/29321271/incremental-distribution-of-strontium-and-zinc-in-great-ape-and-fossil-hominin-cementum-using-synchrotron-x-ray-fluorescence-mapping
#3
Christopher Dean, Adeline Le Cabec, Kathryn Spiers, Yi Zhang, Jan Garrevoet
Cementum and the incremental markings it contains have been widely studied as a means of ageing animals and retrieving information about diet and nutrition. The distribution of trace elements in great ape and fossil hominin cementum has not been studied previously. Synchrotron X-ray fluorescence (SXRF) enables rapid scanning of large tissue areas with high resolution of elemental distributions. First, we used SXRF to map calcium, phosphorus, strontium and zinc distributions in great ape dentine and cementum...
January 2018: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/29313816/osteopontin-regulates-dentin-and-alveolar-bone-development-and-mineralization
#4
B L Foster, M Ao, C R Salmon, M B Chavez, T N Kolli, A B Tran, E Y Chu, K R Kantovitz, M Yadav, S Narisawa, J L Millán, F H Nociti, M J Somerman
The periodontal complex is essential for tooth attachment and function and includes the mineralized tissues, cementum and alveolar bone, separated by the unmineralized periodontal ligament (PDL). To gain insights into factors regulating cementum-PDL and bone-PDL borders and protecting against ectopic calcification within the PDL, we employed a proteomic approach to analyze PDL tissue from progressive ankylosis knock-out (Ank-/-) mice, featuring reduced PPi, rapid cementogenesis, and excessive acellular cementum...
December 4, 2017: Bone
https://www.readbyqxmd.com/read/29311578/promoting-induced-pluripotent-stem-cell-driven-biomineralization-and-periodontal-regeneration-in-rats-with-maxillary-molar-defects-using-injectable-bmp-6-hydrogel
#5
Ke-Hung Chien, Yuh-Lih Chang, Mong-Lien Wang, Jen-Hua Chuang, Ya-Chi Yang, Ming-Cheng Tai, Chien-Ying Wang, Yung-Yang Liu, Hsin-Yang Li, Jiang-Torng Chen, Shou-Yen Kao, Hen-Li Chen, Wen-Liang Lo
Periodontal disease may cause considerable destruction of alveolar bone, periodontal ligaments (PDLs) and cementum and even lead to progressive oral dysfunction. Periodontal tissue regeneration is the ultimate goal of periodontal disease treatment to reconstruct both structures and functions. However, the regenerative efficiency is low, possibly due to the lack of a proper periodontal microenvironment. In this study, we applied an injectable and thermosensitive chitosan/gelatin/glycerol phosphate hydrogel to provide a 3D environment for transplanted stem cells and to enhance stem cell delivery and engraftment...
January 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29288504/cell-therapy-with-allogenic-canine-periodontal-ligament-derived-cells-in-periodontal-regeneration-of-critical-size-defects
#6
J Nuñez, N Sanchez, F Vignoletti, I Sanz-Martin, R Caffesse, S Santamaria, J A Garcia-Sanz, M Sanz
AIM: The objective of this in-vivo experimental study to evaluate the regenerative potential of a cell therapy combining allogenic periodontal ligament derived cells with in a xenogeneic bone substitute in a similar experimental model. METHODS: In 9 beagle dogs, critical-size 6 mm supra-alveolar periodontal defects were created around the PIII and PIV. The resulting supra-alveolar defects were randomly treated with either 1.4 x 106 allogenic canine periodontal ligament-derived cells seeded on de-proteinized bovine bone mineral with 10% collagen (DBBM-C) (test group), or DBBM-C without cells (control group)...
December 29, 2017: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/29287702/scutellaria-baicalensis-ameliorates-the-destruction-of-periodontal-ligament-via-inhibition-of-inflammatory-cytokine-expression
#7
Mi Hye Kim, Haesu Lee, You Yeon Choi, Dae Hoon Lee, Woong Mo Yang
BACKGROUND: Scutellaria baicalensis (SB) is widely used as a medicinal plant to treat various inflammatory diseases. In the present study, we investigated the effects of SB on periodontitis in ligature-induced experimental rat model. METHODS: Rats were subjected to a ligature placement around the first molar of the mandible to induce periodontitis. 100 mg/kg SB extracts were orally administered for 14 days. The molar tissues were stained with 1% methylene blue...
December 26, 2017: Journal of the Chinese Medical Association: JCMA
https://www.readbyqxmd.com/read/29280412/tooth-root-and-alveolar-bone-grey-values-derived-from-cone-beam-ct-imaging-in-maxillary-incisor-tooth-with-and-without-apical-root-resorption
#8
Marcio José da Silva Campos, Karine Simões Silva, Paula Moraes Lupatini, Marcelo Reis Fraga, Robert Willer Farinazzo Vitral
INTRODUCTION: In orthodontic treatment, an increased bone mineral density of the alveolar bone is considered as a risk factor for apical root resorption (ARR), whereas the mineral density of cementum has been associated with root protection against resorption. METHODS: This study aimed at evaluating the grey values (GVs) of the apical third of the root and of the alveolar bone adjacent to maxillary incisors with and without ARR in orthodontic patients. Twenty-one patients under treatment who presented one incisor with ARR and its corresponding contralateral without ARR were selected and submitted to cone-beam computed tomography...
December 27, 2017: Journal of Orthodontics
https://www.readbyqxmd.com/read/29279571/transplantation-of-mature-adipocyte-derived-dedifferentiated-fat-cells-into-three-wall-defects-in-the-rat-periodontium-induces-tissue-regeneration
#9
Daigo Suzuki, Daisuke Akita, Niina Tsurumachi, Koichiro Kano, Katsuyuki Yamanaka, Tadashi Kaneko, Eisuke Kawano, Shinya Iguchi, Taku Toriumi, Yoshinori Arai, Taro Matsumoto, Shuichi Sato, Masaki Honda
The transplantation of dedifferentiated fat (DFAT) cells in combination with poly(d,l-lactic-co-glycolic acid) (PLGA) scaffolds has previously been proven as an effective approach in promoting periodontal tissue regeneration in a rat fenestration defect model. The aim of this study was to assess the regenerative potential of DFAT cells in a rat model of three-wall periodontal bone defect. Three-wall bone defects were created bilaterally on the mesial side of rat maxillary first molars and were either left untreated or treated by implantation of PLGA scaffolds with DFAT cells or PLGA alone...
2017: Journal of Oral Science
https://www.readbyqxmd.com/read/29244957/hypercementosis-associated-with-enpp1-mutations-and-gaci
#10
V Thumbigere-Math, A Alqadi, N I Chalmers, M B Chavez, E Y Chu, M T Collins, C R Ferreira, K FitzGerald, R I Gafni, W A Gahl, K S Hsu, M S Ramnitz, M J Somerman, S G Ziegler, B L Foster
Mineralization of bones and teeth is tightly regulated by levels of extracellular inorganic phosphate (Pi) and pyrophosphate (PPi). Three regulators that control pericellular concentrations of Pi and PPi include tissue-nonspecific alkaline phosphatase (TNAP), progressive ankylosis protein (ANK), and ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1). Inactivation of these factors results in mineralization disorders affecting teeth and their supporting structures. This study for the first time analyzed the effect of decreased PPi on dental development in individuals with generalized arterial calcification of infancy (GACI) due to loss-of-function mutations in the ENPP1 gene...
December 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/29243397/effect-of-strong-tooth-bleaching-with-38-hydrogen-peroxide-on-marginal-seal-of-dental-restorations-using-self-etch-and-total-etch-adhesives
#11
Morakot Piemjai, Spun Lenglerdphol
The purpose of this study was to evaluate the distance and/or thickness of dye penetration at enamel and cementum/dentin margins of restorations before and after vital bleaching. Class V cavities were prepared on extracted human premolars and restored with light-cured resin composite using either AQ-Bond or Single-Bond2 adhesives. Four conditions: no bleaching (control), restored before bleaching (RB), bleached and immediately restored (BIR), bleached and stored in artificial saliva for 7 days then restored (B7R), were used for each adhesive group...
December 15, 2017: Journal of Esthetic and Restorative Dentistry
https://www.readbyqxmd.com/read/29211309/statins-in-nonsurgical-and-surgical-periodontal-therapy-a-systematic-review-and-meta-analysis-of-preclinical-in-vivo-trials
#12
REVIEW
K Bertl, I Steiner, N Pandis, K Buhlin, B Klinge, A Stavropoulos
The cholesterol-lowering drugs, statins, possess anti-inflammatory, antimicrobial and pro-osteogenic properties, and thus have been tested as an adjunct to periodontal treatment. The present systematic review aimed to answer the following focused research question: What is the effect of local and/or systemic statin use on periodontal tissues in preclinical in vivo studies of experimentally induced periodontitis (EIP) and/or acute/chronified periodontal defect (ACP) models? A literature search (of Medline/PubMed, Embase/Ovid, CENTRAL/Ovid) using the following main eligibility criteria was performed: (i) English or German language; (ii) controlled preclinical in vivo trials; (iii) local and/or systemic statin use in EIP and/or ACP models; and (iv) quantitative evaluation of periodontal tissues (i...
December 6, 2017: Journal of Periodontal Research
https://www.readbyqxmd.com/read/29194924/microleakage-around-zirconia-crown-margins-after-ultrasonic-scaling-with-self-adhesive-resin-or-resin-modified-glass-ionomer-cement
#13
Bright Chang, Ronald Goldstein, Chee Paul Lin, Sudha Byreddy, Nathaniel C Lawson
OBJECTIVES: To measure microleakage around zirconia crown margins cemented with self-adhesive resin or resin modified glass ionomer (RMGI) cement after ultrasonic scaling. METHODS: 16 molars were prepared for crowns (margin 0.5 mm coronal of cementum-enamel junction). Preparations were digitally scanned and zirconia crowns milled. Specimens were divided into two groups (n = 8): self-adhesive resin (RelyX Unicem 2) or resin modified glass ionomer (RMGI) (RelyX Luting Plus) cements...
December 1, 2017: Journal of Esthetic and Restorative Dentistry
https://www.readbyqxmd.com/read/29193334/comparison-of-peri-implant-and-periodontal-marginal-soft-tissues-in-health-and-disease
#14
REVIEW
Saso Ivanovski, Ryan Lee
The integrity of the peri-implant soft-tissue seal is crucial for maintaining peri-implant tissue health. Whilst the transmucosal component of the restored implant shares some common features with teeth, namely the presence of a junctional epithelium and a connective tissue component, there are some important differences. A key difference is the nature of the relationship of the connective tissue with the implant surface, whereby there is 'adaptation' of collagen fibers in a parallel orientation in relation to the implant, but insertion of fiber attachment perpendicularly into cementum in the case of teeth...
February 2018: Periodontology 2000
https://www.readbyqxmd.com/read/29178755/prognosis-test-by-visualization-of-demineralized-dentin-under-restorations-to-prevent-initial-wall-lesions-initiated-by-lactic-acid
#15
Morakot Piemjai, Pareewan Chantarawej, Nobuo Nakabayashi, Franklin Garcia-Godoy
PURPOSE: To visualize the complete protection of restored dentin in the acidic environment by the complete impregnation of resin into demineralized dentin. METHODS: Class V cavities prepared in extracted human molars composed of enamel, cementum and dentin surfaces were sealed using either 4-META/MMA-TBB resin with conditioning periods of 10, 30, 60 seconds with 10% citric acid and 3% ferric chloride mixed aqueous solution (10-3) or Single-Bond 2 adhesives prior to restoration with light-cured resin-composite...
June 2017: American Journal of Dentistry
https://www.readbyqxmd.com/read/29170176/rare-presentation-of-cementoblastoma-associated-with-the-deciduous-maxillary-second-molar
#16
Shruti Nagvekar, Shaheen Syed, Anita Spadigam, Anita Dhupar
Cementoblastoma is a benign odontogenic neoplasm accounting for less than 0.69%-8% of all odontogenic tumours and is characterised by the presence of sheets of cementum-like tissue demonstrating large number of reversal lines. It shows an unlimited growth potential and a recurrence rate as high as 37.1%. It most commonly affects the permanent mandibular molars. This paper presents the third reported case of cementoblastoma affecting the deciduous maxillary posterior dentition. A 12-year-old male patient reported to the Department of Oral and Maxillofacial Pathology with a chief complaint of pain and swelling in relation to the deciduous maxillary left second molar...
November 23, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/29161180/comparison-of-verdeluz-orange-g-and-modified-gallego-stains
#17
A Kunche, M A Kiresur, A Ananthaneni, V S Guduru, H K Puneeth, B Bagalad
Tumors of the oral cavity include combinations of hard and soft tissues that may be difficult to identify using routine hematoxylin and eosin (H & E) staining. Although combination stains can demonstrate hard and soft tissues, trichrome stains, such as VanGieson and Masson, cannot differentiate dental hard tissues, such as dentin, cementum and osteoid. Modified Gallegos (MGS) and verdeluz orange G-acid fuchsin (VOF) stains can differentiate components of teeth. We used 10 tissue sections of decalcified bone and 10 pathologic tissue sections that contained different calcified tissues including peripheral ossifying fibroma, odontoma, central ossifying fibroma and cemento-ossifying fibroma...
November 21, 2017: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29120400/fibrin-enhanced-canonical-wnt-signaling-directs-plasminogen-expression-in-cementoblasts
#18
Saeed Ur Rahman, Chan Ho Park, Jeong-Hwa Baek, Hyun-Mo Ryoo, Kyung Mi Woo
Cementum is a mineralized layer on the tooth's root surface and facilitates the biomechanical anchoring of fibrous connective tissues as a part of tooth-supportive complexes. Previously, we observed that OCCM30 cementoblasts cultured on fibrin matrices underwent apoptosis due to fibrin degradation through the expression of proteases. Here, we demonstrated that OCCM30 on fibrin matrices (OCCM30-fibrin) enhanced canonical Wnt signaling, which directed to plasminogen expression. The OCCM30-fibrin showed higher levels of Wnt3a expression, nuclear translocation of β-catenin, and T-cell factor (TCF) optimal motif (TOP) reporter activity than the cells on tissue culture dishes (OCCM30-TCD), indicating that the OCCM30-fibrin enhanced canonical Wnt/β-catenin signaling...
November 9, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29109319/histological-evaluation-of-the-effect-of-platelet-rich-plasma-on-pulp-regeneration-in-nonvital-open-apex-teeth-an-animal-study
#19
Jamileh Ghoddusi, Amir Maghsudlu, Hamid Jafarzadeh, Amirhossein Jafarian, Maryam Forghani
AIM: Platelet-rich plasma (PRP), which is a concentration of growth factors found in platelets, may be a suitable material for pulp regeneration. The aim of this animal study was a histological evaluation of PRP on pulp regeneration in nonvital teeth with immature apices. MATERIALS AND METHODS: A total of 40 premolar dogs' teeth were chosen for this study. After general anesthesia, the teeth were exposed, and subsequently, pulps were removed and the cavities were opened to the oral cavity...
November 1, 2017: Journal of Contemporary Dental Practice
https://www.readbyqxmd.com/read/29098237/-comparative-evaluation-of-marginal-microleakage-of-three-different-resins-in-class-v-composite-restorations
#20
Zheng Zhu, Ya-Qin Zhu
PURPOSE: To compare and evaluate microleakage at the occlusal wall and cervical wall in Class V cavities restored with Ivoclar Tetric N Ceram Bulk Fill composite, Tetric N Flow composite and N Ceram nanocomposite. METHODS: Sixty-six extracted human maxillary premolars, which were intact and healthy, were randomly assigned into 3 groups (n=22). Standardized Class V cavities were prepared on the buccal surface of maxillary premolars. The occlusal walls of the cavities were located on the enamel and the cervical walls were located on dentin and cementum...
June 2017: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
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