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https://www.readbyqxmd.com/read/28097666/understanding-external-cervical-resorption-patterns-in-endodontically-treated-teeth
#1
M Mavridou, E Hauben, M Wevers, E Schepers, L Bergmans, P Lambrechts
AIM: To understand the pattern of external cervical resorption (ECR) in endodontically treated teeth. To compare characteristics and mechanisms of ECR in root filled teeth with those established in teeth with vital pulps. METHODOLOGY: Seven cases of endodontically treated permanent teeth displaying ECR were investigated. ECR diagnosis was based on clinical findings and radiographic examination with cone-beam computed tomography (CBCT). The extracted teeth were further analysed by a nano-focus computed tomographic (nano-CT) system, hard tissue histology and scanning electron microscopy (SEM)...
January 18, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28095448/distribution-of-cathepsin-k-in-late-stage-of-tooth-germ-development-and-its-function-in-degrading-enamel-matrix-proteins-in-mouse
#2
Tao Jiang, Fen Liu, Wei-Guang Wang, Xin Jiang, Xuan Wen, Kai-Jin Hu, Yang Xue
Cathepsin K (CTSK) is a member of cysteine proteinase family, and is predominantly expressed in osteoclastsfor degradationof bone matrix proteins. Given the similarity in physical properties of bone and dental mineralized tissues, including enamel, dentin and cementum, CTSK is likely to take part in mineralization process during odontogenesis. On the other hand, patients with pycnodysostosis caused by mutations of the CTSK gene displayedmultipledental abnormalities, such as hypoplasia of the enamel, obliterated pulp chambers, hypercementosis and periodontal disease...
2017: PloS One
https://www.readbyqxmd.com/read/28070701/use-of-a-continual-sweep-motion-to-compare-air-polishing-devices-powders-and-exposure-time-on-unexposed-root-cementum
#3
Mandy L Herr, Ralph DeLong, Yuping Li, Scott A Lunos, Jill L Stoltenberg
Low abrasive air polishing powders are a viable method for subgingival biofilm removal. This in vitro study evaluated the effects of air polishing using a standard tip on cementum following clinically recommended protocols. Forty-eight teeth were randomly divided into eight groups with six teeth per group. Teeth were treated using either a Hu-Friedy EMS or DENTSPLY Cavitron(®) air polishing device. One of three glycine powders (Air-flow 25 µm, Clinpro 45 μm, Clinpro+TCP 45 μm) or a sodium bicarbonate powder (NaHCO3  85 μm) was sprayed on cementum using a clinically relevant sweeping motion...
January 9, 2017: Odontology
https://www.readbyqxmd.com/read/28068493/bmp1-and-tll1-are-required-for-maintaining-periodontal-homeostasis
#4
J Wang, D Massoudi, Y Ren, A M Muir, S E Harris, D S Greenspan, J Q Feng
Mutations in bone morphogenetic protein 1 (BMP1) in humans or deletion of BMP1 and related protease tolloid like 1 (TLL1) in mice lead to osteogenesis imperfecta (OI). Here, we show progressive periodontal defects in mice in which both BMP1 and TLL1 have been conditionally ablated, including malformed periodontal ligament (PDL) (recently shown to play key roles in normal alveolar bone formation), significant loss in alveolar bone mass ( P < 0.01), and a sharp reduction in cellular cementum. Molecular mechanism studies revealed a dramatic increase in the uncleaved precursor of type I collagen (procollagen I) and a reduction in dentin matrix protein 1 (DMP1), which is partially responsible for defects in extracellular matrix (ECM) formation and mineralization...
January 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28042268/comparing-the-effects-of-manual-and-ultrasonic-instrumentation-on-root-surface-mechanical-properties
#5
Muhammad Sohail Zafar
OBJECTIVE: The aim of the current study is to analyze the surface profiles of healthy and periodontal-treated roots. In addition, manual and ultrasonic instrumentation methods have been compared in terms of surface mechanical properties of root surfaces including surface roughness, hardness, and elastic modulus. MATERIALS AND METHODS: This study was conducted using extracted teeth that were randomly divided into two study groups (1 and 2). Root planing was performed using either Gracey curettes (Group 1) or ultrasonic scaler (Group 2)...
October 2016: European Journal of Dentistry
https://www.readbyqxmd.com/read/27995978/three-dimensional-ultrastructural-analysis-of-cells-in-the-periodontal-ligament-using-focused-ion-beam-scanning-electron-microscope-tomography
#6
Shingo Hirashima, Keisuke Ohta, Tomonoshin Kanazawa, Satoko Okayama, Akinobu Togo, Naohisa Uchimura, Jingo Kusukawa, Kei-Ichiro Nakamura
The accurate comprehension of normal tissue provides essential data to analyse abnormalities such as disease and regenerative processes. In addition, understanding the proper structure of the target tissue and its microenvironment may facilitate successful novel treatment strategies. Many studies have examined the nature and structure of periodontal ligaments (PDLs); however, the three-dimensional (3D) structure of cells in normal PDLs remains poorly understood. In this study, we used focused ion beam/scanning electron microscope tomography to investigate the whole 3D ultrastructure of PDL cells along with quantitatively analysing their structural properties and ascertaining their orientation to the direction of the collagen fibre...
December 20, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27994316/evaluation-of-enterococcus-faecalis-adhesion-penetration-and-method-to-prevent-the-penetration-of-enterococcus-faecalis-into-root-cementum-confocal-laser-scanning-microscope-and-scanning-electron-microscope-analysis
#7
Rahul S Halkai, Mithra N Hegde, Kiran R Halkai
AIM: To ascertain the role of Enterococcus faecalis in persistent infection and a possible method to prevent the penetration of E. faecalis into root cementum. METHODOLOGY: One hundred and twenty human single-rooted extracted teeth divided into five groups. Group I (control): intact teeth, Group II: no apical treatment done, Group III divided into two subgroups. In Groups IIIa and IIIb, root apex treated with lactic acid of acidic and neutral pH, respectively. Group IV: apical root cementum exposed to lactic acid and roughened to mimic the apical resorption...
November 2016: Journal of Conservative Dentistry: JCD
https://www.readbyqxmd.com/read/27994314/comparative-evaluation-of-the-microtensile-bond-strength-of-bulk-fill-and-low-shrinkage-composite-for-different-depths-of-class-ii-cavities-with-the-cervical-margin-in-cementum-an-in-vitro-study
#8
Sonali Taneja, Pragya Kumar, Avnish Kumar
AIM: The aim of this study was to evaluate the microtensile bond strength (μTBS) of bulk fill and low shrinkage composite for different depths of Class II cavities with the cervical margin in cementum. MATERIALS AND METHODS: Standardized conservative box-shaped Class II cavities were prepared on sixty sound-impacted human third molars. The samples were randomly divided into two groups: Group I (n = 30) - horizontal incremental technique and Group II (n = 30) - bulk fill technique (SonicFill)...
November 2016: Journal of Conservative Dentistry: JCD
https://www.readbyqxmd.com/read/27982190/dragon-s-blood-sap-croton-lechleri-as-storage-medium-for-avulsed-teeth-in-vitro-study-of-cell-viability
#9
Christine Men Martins, Elizane Ferreira Hamanaka, Thayse Yumi Hoshida, Ana Maria Sell, Mirian Marubayashi Hidalgo, Catarina Soares Silveira, Wilson Roberto Poi
Tooth replantation success depends on the condition of cementum periodontal ligament after tooth avulsion; which is influenced by storage medium. The dragon's blood (Croton lechleri) sap has been suggested as a promising medium because it supports collagen formation and exhibits healing, anti-inflammatory and antimicrobial properties. Thus, the aim of this study was to evaluate the efficacy of dragon's blood sap as a storage medium for avulsed teeth through evaluation of functional and metabolic cell viability...
October 2016: Brazilian Dental Journal
https://www.readbyqxmd.com/read/27978604/effects-of-emd-liquid-osteogain-on-periodontal-healing-in-class-iii-furcation-defects-in-monkeys
#10
Yoshinori Shirakata, Richard J Miron, Toshiaki Nakamura, Kotaro Sena, Yukiya Shinohara, Naoto Horai, Dieter D Bosshardt, Kazuyuki Noguchi, Anton Sculean
AIM: To evaluate the effect of a novel liquid carrier system of enamel matrix derivative (Osteogain) soaked on an absorbable collagen sponge (ACS) upon periodontal wound healing/regeneration in furcation defects in monkeys. MATERIALS AND METHODS: The stability of the conventional enamel matrix derivative (Emdogain) and Osteogain adsorbed onto ACS was evaluated by ELISA. Chronic class III furcation defects were created at teeth 36, 37, 46, 47 in three monkeys (Macaca fascicularis)...
December 15, 2016: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/27977855/effect-of-mta-particle-size-on-periapical-healing
#11
M Torabinejad, M Moazami, J Hawkins, C Gustefson, H Faras, H Moaddel, K Wright, S Shabahang
AIM: To examine the effect of reduction of MTA particle size on dento-alveolar and osseous healing in dogs. METHODOLOGY: Root canals of 24 mandibular premolars in four 2-year-old beagles were prepared andfilled with gutta-percha and sealer. Two to four weeks later, during periapical surgery, the root-end cavity preparations in these teeth were filled with either gray ProRoot MTA or modified (reduced particle sizes with faster setting time) MTA. The animals were sacrificed four months later...
December 15, 2016: International Endodontic Journal
https://www.readbyqxmd.com/read/27941605/screening-the-expression-changes-in-micrornas-and-their-target-genes-in-mature-cementoblasts-stimulated-with-cyclic-tensile-stress
#12
Liao Wang, Haikun Hu, Ye Cheng, Jianwei Chen, Chongyun Bao, Shujuan Zou, Gang Wu
Cementum is a thin layer of cementoblast-produced mineralized tissue covering the root surfaces of teeth. Mechanical forces, which are produced during masticatory activity, play a paramount role in stimulating cementoblastogenesis, which thereby facilitates the maintenance, remodeling and integrity of cementum. However, hitherto, the extent to which a post-transcriptional modulation mechanism is involved in this process has rarely been reported. In this study, a mature murine cementoblast cell line OCCM-30 cells (immortalized osteocalcin positive cementoblasts) was cultured and subjected to cyclic tensile stress (0...
December 7, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27939733/mesoporous-calcium-silicate-nanoparticles-with-drug-delivery-and-odontogenesis-properties
#13
Ching-Yuang Huang, Tsui-Hsien Huang, Chia-Tze Kao, Yuan-Haw Wu, Wan-Chen Chen, Ming-You Shie
INTRODUCTION: Calcium silicate (CS) -based materials play an important role in the development of endodontic materials that induce bone/cementum tissue regeneration and inhibit bacterial viability. The aim of this study was to prepare novel mesoporous CS (MesoCS) nanoparticles that have osteogenic, drug delivery, and antibacterial characteristics for endodontic materials and also have an excellent ability to develop apatite mineralization. METHODS: The MesoCS nanoparticles were prepared using sol-gel methods...
January 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/27936010/new-insights-on-the-composition-and-the-structure-of-the-acellular-extrinsic-fiber-cementum-by-raman-analysis
#14
Thomas Colard, Guillaume Falgayrac, Benoit Bertrand, Stephan Naji, Olivier Devos, Clara Balsack, Yann Delannoy, Guillaume Penel
Acellular extrinsic fiber cementum is a mineralized tissue that covers the cervical half of the tooth root surface. It contains mainly extrinsic or Sharpey's fibers that run perpendicular to the root surface to anchor the tooth via the periodontal ligament. Acellular cementum is continuously and slowly produced throughout life and exhibits an alternating bright and dark pattern under light microscopy. However, although a better understanding of the structural background of acellular cementum is relevant to many fields, such as cementochronology, periodontology and tissue engineering, acellular cementum remains rarely studied and poorly understood...
2016: PloS One
https://www.readbyqxmd.com/read/27924608/a-method-to-isolate-purify-and-characterize-human-periodontal-ligament-stem-cells
#15
Krzysztof Mrozik, Stan Gronthos, Songtao Shi, P Mark Bartold
Human periodontal ligament stem cells (PDLSCs) are a unique population of mesenchymal stem cells (MSCs) that demonstrate the capacity to generate cementum- and periodontal ligament-like structures in vivo. As such, PDLSCs represent a promising cell-based therapy in reconstructive dentistry for the treatment of periodontal disease. The present chapter describes two methods for isolating PDLSCs from human PDL tissue including traditional plastic adherence, and immunomagnetic selection based on the expression of MSC-associated surface markers STRO-1 antigen, CD146 (MUC-18), CD29 (Integrin β-1), CD44, and CD106 (VCAM-1)...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27909868/dental-age-estimation-in-the-living-after-completion-of-third-molar-mineralization-new-data-for-gustafson-s-criteria
#16
M Timme, W H Timme, A Olze, C Ottow, S Ribbecke, H Pfeiffer, R Dettmeyer, A Schmeling
There is a need for dental age estimation methods after completion of the third molar mineralization. Degenerative dental characteristics appear to be suitable for forensic age diagnostics beyond the 18th year of life. In 2012, Olze et al. investigated the criteria studied by Gustafson using orthopantomograms. The objective of this study was to prove the applicability and reliability of this method with a large cohort and a wide age range, including older individuals. For this purpose, 2346 orthopantomograms of 1167 female and 1179 male Germans aged 15 to 70 years were reviewed...
December 1, 2016: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/27905816/cementocyte-cell-death-occurs-in-rat-cellular-cementum-during-orthodontic-tooth-movement
#17
Humihiro Matsuzawa, Naoko Toriya, Yuya Nakao, Moe Konno-Nagasaka, Toshiya Arakawa, Miki Okayama, Itaru Mizoguchi
OBJECTIVE: To clarify the mechanism of root resorption during orthodontic treatment, we examined cementocyte cell death and root resorption in the cellular cementum on the pressure side during experimental tooth movement. MATERIALS AND METHODS: Using 8-week-old male Wistar rats, the right first molar was pushed mesiobuccally with a force of 40 g by a Ni-Ti alloy wire while the contralateral first molar was used as a control. Localization and number of cleaved caspase-3-positive and single-stranded DNA (ssDNA) - positive cells were evaluated using dual-label immunohistochemistry with anticleaved caspase-3 and anti-ssDNA antibodies...
December 1, 2016: Angle Orthodontist
https://www.readbyqxmd.com/read/27897460/current-evidence-on-the-socket-shield-technique-a-systematic-review
#18
Amit Srikant Gharpure, Neel B Bhatavadekar
The recently popularised socket shield technique involves intentional retention of a thin buccal section of the remnant root at the time of immediate implant placement to preserve the buccal crestal bone from resorption. The objective of this systematic review was to assess the literature available on the socket-shield technique and weigh its biological plausibility and long-term clinical prognosis. A Systematic Search was performed in PubMed-Medline, Embase, Web of Knowledge, Google Scholar and Cochrane Central for clinical/ animal studies up to May 2016 without restrictions of language, duration, and follow-up...
November 29, 2016: Journal of Oral Implantology
https://www.readbyqxmd.com/read/27871286/maxillary-calcifying-epithelial-odontogenic-tumor-with-sinus-and-buccal-vestibule-extension-a-case-report-and-immunohistochemical-study
#19
Cristina Munteanu, Daniel Pirici, Alex Emilian Stepan, Adrian Camen, Claudiu Margaritescu
BACKGROUND: Calcifying epithelial odontogenic tumor (CEOT) is a rare benign neoplasia, locally aggressive, that tends to invade bone and adjacent soft tissues. This case report describes the thirteenth known case of CEOT with maxillary sinus extension and the second one that also involves the buccal vestibule mucosa with peculiar histopathological and immunohistochemical data. CASE PRESENTATION: Here we report the case of a 45-year-old female with a CEOT diagnosed and treated at the Oral & Maxillofacial Surgery Department, County Clinical Emergency Hospital of Craiova, Romania...
November 21, 2016: Diagnostic Pathology
https://www.readbyqxmd.com/read/27860247/preservation-of-root-cementum-a-comparative-evaluation-of-power-driven-versus-hand-instruments
#20
E Bozbay, F Dominici, A Y Gokbuget, S Cintan, L Guida, M S Aydin, A Mariotti, A Pilloni
OBJECTIVES: The purpose of this study was to evaluate the effects of three distinct periodontal treatment methods in comparison with hand instrumentation on residual cementum of periodontal diseased teeth. Cementum can influence the activities of periodontal cells and may play an important regulatory role in periodontal treatment. The ideal method for periodontal therapy involves removal of biofilm, calculus and endotoxin while preserving root cementum. MATERIAL AND METHODS: Forty-eight caries free, single-rooted teeth in patients diagnosed with severe chronic periodontitis were treated using four different methods prior to extraction...
November 16, 2016: International Journal of Dental Hygiene
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