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https://www.readbyqxmd.com/read/28531913/methods-of-isolation-and-characterization-of-stem-cells-from-different-regions-of-oral-cavity-using-markers-a-systematic-review
#1
REVIEW
Kavarthapu Avinash, Sankari Malaippan, Jayakumar Nadathur Dooraiswamy
Background: Periodontitis is a destructive inflammatory disorder of the periodontium caused by the destruction of periodontal tissues namely the PDL, cementum, alveolar bone, and gingiva. Once these tissues are lost, the foremost goal of periodontal therapy is to regenerate the diseased tissues if possible to their original form, architecture, and function. Various regenerative procedures were employed and still a gap was found in achieving the goal. As stem cells are characterized by their ability to self-renew and differentiate to produce specialized cells, there could be a possibility of using them for regenerative therapy...
May 30, 2017: International Journal of Stem Cells
https://www.readbyqxmd.com/read/28528307/extracellular-atp-is-a-key-modulator-of-alveolar-bone-loss-in-periodontitis
#2
REVIEW
Itzhak Binderman, Nasir Gadban, Avinoam Yaffe
Periodontal diseases are initiated by pathogenic bacterial biofilm activity that induces a host inflammatory cells immune response, degradation of dento gingival fibrous tissue and its detachment from root cementum. It is well accepted, that osteoclastic alveolar bone loss is governed exclusively through secretion of proinflammatory cytokines. Nevertheless, our findings suggest that once degradation of collagen fibers by MMPs occurs, a drop of cellular strains cause immediate release of ATP from marginal gingival fibroblasts, cell deformation and influx of Ca+2...
May 13, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28528141/portland-cement-induces-human-periodontal-ligament-cells-to-differentiate-by-upregulating-mir-146a
#3
Min-Ching Wang, Li-Yin Yeh, Wen-Yu Shih, Wan-Chun Li, Kuo-Wei Chang, Shu-Chun Lin
BACKGROUND/PURPOSE: Bioaggregates such as Portland cement (PC) can be an economical alternative for mineral trioxide aggregate (MTA) with additional benefit of less discoloration. MTA has been known to induce differentiations of several dental cells. MicroRNAs are important regulators of biological processes, including differentiation, physiologic homeostasis, and disease progression. This study is to explore how PC enhances the differentiation of periodontal ligament (PDL) cells in microRNAs level...
May 18, 2017: Journal of the Formosan Medical Association, Taiwan Yi Zhi
https://www.readbyqxmd.com/read/28527949/microproteome-of-dentoalveolar-tissues
#4
Cristiane R Salmon, Ana Paula O Giorgetti, Adriana F Paes Leme, Romênia R Domingues, Tamara N Kolli, Brian L Foster, Francisco H Nociti
Proteomic analysis of extracellular matrices (ECM) of dentoalveolar tissues can provide insights into developmental, pathological, and reparative processes. However, targeted dissection of mineralized tissues, dental cementum (DC), alveolar bone (AB), and dentin (DE), presents technical difficulties. We demonstrate an approach combining EDTA decalcification and laser capture microdissection (LCM), followed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), to analyze proteome profiles of these tissues...
May 17, 2017: Bone
https://www.readbyqxmd.com/read/28517970/osteoblast-progenitors-enhance-osteogenic-differentiation-of-periodontal-ligament-stem-cells
#5
Miao Yu, Limei Wang, Pengfei Ba, Linxia Li, Long Sun, Xiaoqi Duan, Pishan Yang, Chengzhe Yang, Qinfeng Sun
BACKGROUND: Osteoblasts and periodontal ligament stem cells (PDLSCs) play an important role in maintaining physiological function of periodontal tissues and participating in periodontal regeneration. Elucidation of interactions between osteoblasts and PDLSCs will aid understanding of periodontal regeneration mechanisms. This study aimed to determine whether pre-osteoblasts can promote osteoblastic/cementoblastic differentiation of PDLSCs. METHODS: PDLSCs were cultured alone (control group), or co-cultured indirectly with human gingival fibroblasts (HGFs) (HGFs group) or MC3T3-E1 cells (OB groups)...
May 18, 2017: Journal of Periodontology
https://www.readbyqxmd.com/read/28476468/does-the-removal-of-cementum-facilitate-bacterial-penetration-into-dentinal-tubules-in%C3%A2-vitro
#6
Asma Alyahya, Athbi Alqareer
INTRODUCTION: The importance of an intact layer of cementum on the root surface in preventing bacterial penetration into radicular dentin has not been sufficiently investigated. The aim of this in vitro study was to determine the effect of the absence of cementum from the root surface and the length of the infection period (2 or 4 weeks) on the maximum depth of bacterial penetration and the percentage of sectors lined with bacteria. METHODS: Sound, single-rooted extracted teeth with closed apices were randomly assigned to either a control group (cementum present [CP]) or an experimental group (cementum removed [CR])...
May 2, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28476202/periodontal-ligament-entheses-and-their-adaptive-role-in-the-context-of-dentoalveolar-joint-function
#7
REVIEW
Jeremy D Lin, Andrew T Jang, Michael P Kurylo, Jonathan Hurng, Feifei Yang, Lynn Yang, Arvin Pal, Ling Chen, Sunita P Ho
OBJECTIVE: The dynamic bone-periodontal ligament (PDL)-tooth fibrous joint consists of two adaptive functionally graded interfaces (FGI), the PDL-bone and PDL-cementum that respond to mechanical strain transmitted during mastication. In general, from a materials and mechanics perspective, FGI prevent catastrophic failure during prolonged cyclic loading. This review is a discourse of results gathered from literature to illustrate the dynamic adaptive nature of the fibrous joint in response to physiologic and pathologic simulated functions, and experimental tooth movement...
May 2, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/28447749/dental-and-periodontal-phenotypes-of-dlx2-overexpression-in-mice
#8
Jiewen Dai, Jiawen Si, Ningjuan Ouyang, Jianfei Zhang, Dandan Wu, Xudong Wang, Guofang Shen
Distal-less homeobox 2 (Dlx2) is a member of the homeodomain family of transcription factors and is important for the development of cranial neural crest cells (CNCCs)‑derived craniofacial tissues. Previous studies revealed that Dlx2 was expressed in the cementum and a targeted null mutation disrupted tooth development in mice. However, whether Dlx2 overexpression may impair in vivo tooth morphogenesis remains to be elucidated. The present study used a transgenic mouse model to specifically overexpress Dlx2 in neural crest cells in order to identify the dental phenotypes in mice by observation, micro‑computed tomography and histological examination...
May 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28446370/microcarrier-culture-enhances-osteogenic-potential-of-human-periodontal-ligament-stromal-cells
#9
Alina Čebatariūnienė, Akvilė Jarmalavičiūtė, Virginijus Tunaitis, Alina Pūrienė, Algirdas Venalis, Augustas Pivoriūnas
Regeneration of periodontal tissue represents a major challenge to modern tissue engineering, since cell-based therapies require large amounts of periodontal ligament stromal cells (PLSC), which can be obtained only by in vitro expansion. Ideally, the period of the in vitro expansion should be optimized for the generation of large enough numbers of pre-specified progenitor cells ready to contribute to the restoration of periodontal tissues. In the present study, we used a commercially available, three-dimensional culturing platform and alginate microcarrier cell culture system for the propagation of human PLSCs, which were derived using the explant outgrowth method...
March 29, 2017: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/28440742/leptin-overexpression-in-bone-marrow-stromal-cells-promotes-periodontal-regeneration-in-a-rat-model-of-osteoporosis
#10
Baoyu Zheng, Jun Jiang, Yuling Chen, Minkui Lin, Zhibin Du, Yin Xiao, Kai Luo, Fuhua Yan
BACKGROUND: Osteoporosis (OP) is associated with widespread periodontitis and impaired periodontal healing. However, there is a lack of information about the outcomes of regenerative approaches under the influence of OP. This study investigated the effect of leptin (LEP) overexpression on the regenerative potential of bone marrow stromal cells (BMSCs) in an osteoporotic rat periodontal fenestration defect model. METHODS: Rat BMSCs were transfected with adenoviruses harboring the human (h) LEP gene...
April 25, 2017: Journal of Periodontology
https://www.readbyqxmd.com/read/28437177/periodontal-derived-mesenchymal-cell-sheets-promote-periodontal-regeneration-in-inflammatory-microenvironment
#11
Shujuan Guo, Jian Kang, Baohui Ji, Weihua Guo, Yi Ding, Yafei Wu, Weidong Tian
In this study, we investigated the periodontal regenerative potential of dental follicle cell (DFC) sheets and periodontal ligament cell (PDLC) sheets in the simulating inflammatory microenvironment of periodontitis, to confirm their regenerative potential for clinical application and explain the possible mechanism. The biological characteristics of DFC sheets and PDLC sheets were explored in lipopolysaccharide from Porphyromonas gingivalis (P. gingivalis LPS)-induced inflammation microenvironment in vitro, then cell sheets were transplanted into canine periodontal defects with experimental periodontitis in situ for 3 months...
May 18, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28429478/the-nexus-between-periodontics-and-oral-pathology
#12
REVIEW
Alison M Rich, Benedict Seo, Venkata Parachuru, Haizal M Hussaini
A wide variety of lesions may arise from the oral mucosa, fibrous connective tissue, bone and cementum of the periodontium. The commonest pathology occurs as a result of bacterial infection and is very well known to dentists and periodontists, but rarer conditions present as gingival pathology. The pathogenesis of these conditions ranges from genetic to traumatic to immunological to neoplastic, and includes benign, malignant and metastatic lesions. This paper outlines some of these conditions and describes how the periodontist and oral pathologist can work together using a framework, and how with careful consideration of the clinical features and the use of appropriate special tests, including obtaining an adequate tissue specimen, a timely and accurate diagnosis can be obtained...
June 2017: Periodontology 2000
https://www.readbyqxmd.com/read/28427342/intermittent-parathyroid-hormone-pth-promotes-cementogenesis-and-alleviates-the-catabolic-effects-of-mechanical-strain-in-cementoblasts
#13
Yuyu Li, Zhiai Hu, Chenchen Zhou, Yang Xu, Li Huang, Xin Wang, Shujuan Zou
BACKGROUND: External root resorption, commonly starting from cementum, is a severe side effect of orthodontic treatment. In this pathological process and repairing course followed, cementoblasts play a significant role. Previous studies implicated that parathyroid hormone (PTH) could act on committed osteoblast precursors to promote differentiation, and inhibit apoptosis. But little was known about the role of PTH in cementoblasts. The purpose of this study was to investigate the effects of intermittent PTH on cementoblasts and its influence after mechanical strain treatment...
April 20, 2017: BMC Cell Biology
https://www.readbyqxmd.com/read/28422843/hypoxia-regulated-human-periodontal-ligament-cells-via-wnt-%C3%AE-catenin-signaling-pathway
#14
Zhili Xiao, Yineng Han, Yan Zhang, Xiaonan Zhang
BACKGROUND: The aim of this study is to investigate the effects of hypoxia on the proliferation, morphology, migration ability, hypoxia inducible factor (HIF) 1 (HIF-1) expression, and the relationship with Wnt/β-catenin signaling of human periodontal ligament cells (hPDLCs) in vitro. METHODS: hPDLCs (4th passage) cultured by the tissue culture method were randomly assigned to slight (5% O2), severe hypoxia (1% O2) groups, and the control (21% O2) group, respectively...
April 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28416302/effect-of-bacterial-biofilm-on-the-osteogenic-differentiation-of-stem-cells-of-apical-papilla
#15
Lakshmi Vishwanat, Rose Duong, Koyo Takimoto, Linda Phillips, Claudia O Espitia, Anibal Diogenes, Shivani B Ruparel, David Kolodrubetz, Nikita B Ruparel
INTRODUCTION: Although clinical success in regenerative endodontics is substantially high, histological success is limited to finding bone/cementum-like tissue instead of dentin within the canal space. The aims of this study were to investigate (1) the effect of bacterial biofilm on osteogenic gene expression in stem cells of the apical papilla (SCAP) and (2) the effect of bacterial antigens on the functional differentiation of SCAP into a mineralizing phenotype. METHODS: Using an ex vivo organotypic root canal model and an American Association of Endontists-recommended regenerative endodontic procedures, we evaluated SCAP differentiation in the presence and absence of an Enterococcus faecalis biofilm...
April 14, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28408958/histology-of-human-cementum-its-structure-function-and-development
#16
REVIEW
Tsuneyuki Yamamoto, Tomoka Hasegawa, Tomomaya Yamamoto, Hiromi Hongo, Norio Amizuka
Cementum was first demonstrated by microscopy, about 180 years ago. Since then the biology of cementum has been investigated by the most advanced techniques and equipment at that time in various fields of dental sciences. A great deal of data on cementum histology have been accumulated. These data have been obtained from not only human, but also non-human animals, in particular, rodents such as the mouse and rat. Although many dental histologists have reviewed histology of human cementum, some descriptions are questionable, probably due to incorrect comparison of human and rodent cementum...
August 2016: Japanese Dental Science Review
https://www.readbyqxmd.com/read/28407986/intravenous-bisphosphonate-therapy-does-not-thicken-cementum-or-change-periodontal-ligaments-of-cancer-patients
#17
Mariana de Pauli Paglioni, Wagner Gomes Silva, Juliana Pereira, César Augusto Migliorati, Marcio Ajudarte Lopes, Oslei Paes de Almeida, Mario Fernando Goes, Ana Carolina Prado Ribeiro, Thais Bianca Brandão, Alan Roger Santos-Silva
OBJECTIVE: To test the hypothesis that intravenous (IV) bisphosphonate (BP) therapy thickens or alters the micromorphology of cementum and periodontal ligament (PDL) in cancer patients. STUDY DESIGN: Thirty-two teeth extracted from 24 cancer patients and separated into test (patients who have undergone IV BP therapy, n = 16) and control (patients naive to BP therapy, n = 16) groups were studied. Cementum thickness was measured in 3 different areas of the dental root with polarized light microscopy...
May 2017: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
https://www.readbyqxmd.com/read/28395716/-insulin-like-growth-factor-1-promotes-the-proliferation-and-differentiation-of-human-periodontal-ligament-stem-cells-into-periodontal-ligament-fibroblasts
#18
Junxiu Qu, Lihui Jia, Caixin Che, Yuanyuan Yue, Jiangna Zhu, Chao Liu
Objective To investigate the effect of insulin-like growth factor 1 (IGF-1) on the proliferation and differentiation potential of human periodontal ligament stem cells (hPDLSCs). Methods The hPDLSCs were isolated by the limited dilution method. Surface markers of hPDLSCs were detected by flow cytometry. Cell clones were tested by crystal violet staining. The hPDLSCs were induced into osteoblasts and adipogenic by osteogenic and adipogenic medium. The influence of different concentrations of IGF-1 (1, 10, 30, 50, 100 ng/mL) on hPDLSCs was analyzed by CCK-8 assay...
April 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28394043/efficacy-of-stem-cells-on-periodontal-regeneration-systematic-review-of-pre-clinical-studies
#19
REVIEW
S A Tassi, N Z Sergio, M Y O Misawa, C C Villar
This systematic review aims to evaluate mesenchymal stem cells (MSC) periodontal regenerative potential in animal models. MEDLINE, EMBASE and LILACS databases were searched for quantitative pre-clinical controlled animal model studies that evaluated the effect of local administration of MSC on periodontal regeneration. The systematic review was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses statement guidelines. Twenty-two studies met the inclusion criteria. Periodontal defects were surgically created in all studies...
April 10, 2017: Journal of Periodontal Research
https://www.readbyqxmd.com/read/28361377/alveolar-ridge-preservation-with-autologous-particulated-dentin-a-case-series
#20
Silvio Valdec, Pavla Pasic, Alex Soltermann, Daniel Thoma, Bernd Stadlinger, Martin Rücker
INTRODUCTION: Ridge preservation can be performed with autologous bone, alloplastic bone substitute material or a combination of both. Dentin is similar to bone in its chemical composition. In its use as bone substitute material, it undergoes a remodelling process and transforms to bone. The presented case report introduces a technique in which the extraction socket is augmented with autologous, particulated dentin. MATERIAL AND METHODS: The fractured, non-savable mesial incisor of the upper jaw was carefully extracted in axial direction...
December 2017: International Journal of Implant Dentistry
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