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Lilian Vieira Oliveira, Thais Souza Maia, Karla Zancopé, Murilo de Souza Menezes, Carlos José Soares, Camilla Christian Gomes Moura
The presence of residues within the root canal after post-space preparation can influence the bond strength between resin cement and root dentin when using fiberglass posts (FGPs). Currently, there is no consensus in the literature regarding what is the best solution for the removal of debris after post-space preparation. This systematic review involved "in vitro" studies to investigate if cleaning methods of the root canal after post-space preparation can increase the retention of FGPs evaluated by the push-out test...
March 15, 2018: Brazilian Oral Research
Karl-Anton Hiller, Wolfgang Buchalla, Isabel Grillmeier, Christina Neubauer, Gottfried Schmalz
This in vitro study evaluated the effect of toothpastes with different active ingredients on dentin permeability using an extended protocol including multiple applications and several thermal ageing cycles in the presence or absence of human saliva. The Null hypothesis was that dentin permeability of a hydroxyapatite containing toothpaste (BR), a potassium nitrate (SP) and an arginine and calcium carbonate (EH) containing toothpaste were similar. Dentin permeability was measured as hydraulic conductance using a commercially available capillary flow system (Flodec, Geneva) and results were expressed as % relative to matching controls...
March 20, 2018: Scientific Reports
Ines Kallel, Eya Moussaoui, Fadwa Chtioui, Nabiha Douki
According to the American Association of Endodontists, "a 'true' vertical root fracture is defined as a complete or incomplete fracture initiated from the root at any level, usually directed buccolingually." Vertical root fracture (VRF) usually starts from an internal dentinal crack and develops over time, due to masticatory forces and occlusal loads. When they occur in teeth, those types of fractures can present difficulties in diagnosis, and there are however many clinic and radiographical signs which can guide clinicians to the existence of the fracture...
2018: Case Reports in Dentistry
Luiz Filipe Barbosa-Martins, Jossaria Pereira de Sousa, Aline Rogéria Freire de Castilho, Julia Puppin-Rontani, Robert P W Davies, Regina Maria Puppin-Rontani
Bonding to demineralized dentin of a diseased tooth has shown to be a significant clinical issue. This study evaluated the effect of 0.2% NaF-(NaF), MI Paste™-(CPP-ACP) and the self-assembling peptide 'P11 - 4 ' (Ace-QQRFEWEFEQQ-NH2 ) contained in Curodont™ Repair, have on microtensile bond strength-(µTBS) of two different adhesive systems (Adper™ Single Bond-(SB) or Clearfil™ SE Bond (CSE)) and wettability of demineralized dentin slices after remineralising agents were applied. The highest µTBS were found for the demineralized dentin-(DD) treated with CPP-ACP; both adhesives systems (p < 0...
March 10, 2018: Journal of the Mechanical Behavior of Biomedical Materials
Zhejun Wang, Ya Shen, Markus Haapasalo
INTRODUCTION: Conventional endodontic treatment includes instrumentation of the canals in most cases to size #25/.06 or larger, which changes the original canal wall anatomy. In recent years, energy-driven equipment, such as photon-induced photoacoustic streaming (Fotona LLC, Dallas, TX) and a multisonic GentleWave system (Sonendo Inc, Laguna Hills, CA), have been introduced to facilitate cleaning of minimally instrumented canals or even uninstrumented canals. The purpose of this study was to examine root canal wall anatomy in premolar teeth cleaned by a noninstrumentation method after #10 K-file patency examination...
March 14, 2018: Journal of Endodontics
Sanupong Prompreecha, Thanapat Sastraruji, Phumisak Louwakul, Tanida Srisuwan
INTRODUCTION: The use of dentin preconditioning techniques in regenerative endodontic procedures is currently promising. Several growth factors have been detected on dentin after ultrasonic irrigation with EDTA. This study aimed to evaluate the effects of dynamic irrigation with different solution regimens on apical papilla cell (APC) attachment in an ex vivo immature tooth model. METHODS: Various dynamic irrigation techniques, needle irrigation (NI), NI with EndoActivator, and NI with passive ultrasonic irrigation, were used with different solution regimens, normal saline solution (NSS), EDTA, and chlorhexidine digluconate followed by EDTA, in enlarged root canal models where calcium hydroxide-medicated dentin slices were inserted...
March 14, 2018: Journal of Endodontics
Stefanie Amend, Roland Frankenberger, Susanne Lücker, Eugen Domann, Norbert Krämer
OBJECTIVES: The present study aimed to establish Lactobacillus casei in a completely automated Streptococcus mutans-based artificial mouth model and to investigate secondary caries inhibiting properties of glass ionomer cements / resin composite groups in vitro. METHODS: Sixty extracted, caries-free human third molars were used for preparation of standardized class-V-cavities. Specimens were restored with a resin-modified (Photac Fil; PF) as well as a conventional glass ionomer cement (Ketac Molar; KM) and one resin composite bonded with and without conduction of etch-and-rinse technique (Prime&Bond NT, Ceram X mono; C+ERT, C)...
March 12, 2018: Dental Materials: Official Publication of the Academy of Dental Materials
Victor Feliz Pedrinha, Juliana Melo da Silva Brandão, Oscar Faciola Pessoa, Patrícia de Almeida Rodrigues
Advances in endodontics have enabled the evolution of file manufacturing processes, improving performance beyond that of conventional files. In the present study, systems manufactured using state of the art methods and possessing special properties related to NiTi alloys ( i.e ., CM-Wire, M-Wire and R-Phase) were selected. The aim of this review was to provide a detailed analysis of the literature about the relationship between recently introduced NiTi files with different movement kinematics and shaping ability, apical extrusion of debris and dentin defects in root canal preparations...
2018: Open Dentistry Journal
Jorge Henrique Stefaneli Marques, Yara Teresinha Corrêa Silva-Sousa, Fuad Jacob Abi Rached-Júnior, Luciana Martins Domingues de Macedo, Jardel Francisco Mazzi-Chaves, Josette Camilleri, Manoel Damião Sousa-Neto
The aim of this study was to evaluate the bond strength of different triccalcium silicate cements to retrograde cavity using a push out test. Thirty maxillary central incisors were shaped using #80 hand files and sectioned transversally. Root slices were obtained from the apical 4 mm after eliminating the apical extremity. The specimens were embedded in acrylic resin and positioned at 45° to the horizontal plane for preparation of root-end cavities with a diamond ultrasonic retrotip. The samples were divided into three groups according to the root-end filling material (n = 10): MTA Angelus, ProRoot MTA and Biodentine...
March 8, 2018: Brazilian Oral Research
Takahiko Niwa, Yasuo Yamakoshi, Hajime Yamazaki, Takeo Karakida, Risako Chiba, Jan C-C Hu, Takatoshi Nagano, Ryuji Yamamoto, James P Simmer, Henry C Margolis, Kazuhiro Gomi
Transforming growth factor-beta (TGF-β) is critical for cell proliferation and differentiation in dental pulp. Here, we show the dynamic mechanisms of TGF-β in porcine dental pulp, odontoblasts and dentin. The mRNA of latent TGF-β1 and TGF-β3 is predominantly expressed in odontoblasts, whereas the mRNA expression level of latent TGF-β2 is high in dental pulp. TGF-β1 is a major isoform of TGF-β, and latent TGF-β1, synthesized in dental pulp, is primarily activated by matrix metalloproteinase 11 (MMP11)...
March 13, 2018: Scientific Reports
Anamaria Balic
The tooth is an intricate composition of precisely patterned, mineralized matrices and soft tissues. Mineralized tissues include enamel (produced by the epithelial cells called ameloblasts), dentin and cementum (produced by mesenchymal cells called odontoblasts and cementoblasts, respectively), and soft tissues, which include the dental pulp and the periodontal ligament along with the invading nerves and blood vessels. It was perceived for a very long time that teeth primarily serve an esthetical function. In recent years, however, the role of healthy teeth, as well as the impact of oral health on general well-being, became more evident...
March 13, 2018: Gerontology
M Wolf, M Ao, M B Chavez, T N Kolli, V Thumbigere-Math, K Becker, E Y Chu, A Jäger, M J Somerman, B L Foster
Previous studies revealed that cementum formation is tightly regulated by inorganic pyrophosphate (PPi ), a mineralization inhibitor. Local PPi concentrations are determined by regulators, including ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), which increases PPi concentrations by adenosine triphosphate hydrolysis. Orthodontic forces stimulate alveolar bone remodelling, leading to orthodontic tooth movement (OTM). To better understand how disturbed mineral metabolism and the resulting altered periodontal structures affect OTM, we employed Enpp1 mutant mice that feature reduced PPi and increased cervical cementum in a model of OTM induced by a stretched closed-coil spring ligated between the maxillary left first molar and maxillary incisors...
March 1, 2018: Journal of Dental Research
Severine L. Staub, Eckart Teubner, Nicola U. Zitzmann
The aim of this retrospective study was to investigate the clinical outcome of Dalbo®-Rotex® retention elements in a private practice. The majority of the retention elements was fixed with self-adhesive composite cement, and in almost 40% the exposed root surface was additionally covered with composite. The success rate of 96.3% after 24 months in a total of 50 included patients (response rate 48.5%) demonstrated that a simple and cost-effective fixation of removable dentures using prefabricated, chair-side inserted retention elements is feasible...
March 12, 2018: Swiss Dental Journal
Pune N Tawakoli, Klaus Becker, Thomas Attin
This study was to analyse the abrasive wear of differently composed diamond dentifrices loaded with 2.4 μm diamond particles on dentine and enamel surfaces in vitro. Bovine specimens were brushed with a diamond-loaded dentifrice (DD2; 2 g particles/kg), a diamond-loaded dentifrice (1.5 g/kg) containing 20% hydrated silica as extra abrasive (DD1.5+S), or a diamond-loaded dentifrice (3 g/kg) containing 20% hydrated silica abrasive (DD3+S). Values were compared to those obtained with Colgate Total (CT) and Elmex Sensitive plus (ES)...
January 15, 2018: Swiss Dental Journal
Yaling Liu, Tulika Sharma, I-Ping Chen, Ernst Reichenberger, Yasuyoshi Ueki, Yumna Arif, Daniel Parisi, Peter Maye
We utilized a bone marrow stromal culture system to investigate changes in TGFβ signaling in a mouse model for cherubism (Sh3bp2KI/KI ). Interestingly, bone marrow cultures derived from cherubism mice not only displayed impaired osteoblast differentiation, but also had spontaneous osteoclast formation. PAI1, a target gene of TGFβ signaling, was elevated 2-fold in cherubism CD11b- ,CD45- cells compared to wild type cells, while the expression of BAMBI, an inhibitor of TGFβ signaling, was down-regulated. We also discovered that treatment of cherubism cultures with antagonists of the TGFβ signaling pathway could largely rescue osteoblast differentiation and markedly reduce spontaneous osteoclast formation...
March 9, 2018: Bone
Priyam Jani, Chao Liu, Hua Zhang, Khaled Younes, M Douglas Benson, Chunlin Qin
OBJECTIVE: The bone morphogenetic proteins (BMPs) play crucial roles in tooth development. However, several BMPs retain expression in the dentin of the fully patterned and differentiated tooth. We hypothesized that BMP signaling therefore plays a role in the function of the differentiated odontoblast, the job of which is to lay down and mineralize the dentin matrix. DESIGN: We generated mice deficient in Bmp2 and 4 using a dentin matrix protein 1 (Dmp1) promoter-driven cre recombinase that was expressed in differentiated odontoblasts...
February 7, 2018: Archives of Oral Biology
Karim Elhennawy, Haitham Askar, Paul-Georg Jost-Brinkmann, Seif Reda, Allam Al-Abdi, Sebastian Paris, Falk Schwendicke
OBJECTIVES: To assess the accuracy of near-infrared-light transillumination (DIAGNO) compared to visual-tactile (VT) and radiographic (RA) evaluation of proximal carious lesions adjacent to composite restorations in vitro. METHODS: Two hundred extracted posterior permanent human teeth with occluso-proximal composite restorations were allocated to 50 groups of four posterior teeth, and mounted in a pilot-tested diagnostic model in a dummy head. Teeth were independently assessed by two examiners...
March 8, 2018: Journal of Dentistry
Sang-Joun Yu, Yoon Seon Lee, Han-Wool Choung, Yeoung-Hyun Park, Byung-Ock Kim, Joo-Cheol Park
Preameloblast-conditioned medium (PACM) has been reported as a potent dentin regenerative material, but its effects as a mixture on periodontal regeneration and the role of CPNE7 in PACM are not known. The purpose of this study is to evaluate the histologic and histomorphometric effects of preameloblast-conditioned medium (PACM) and CPNE7 on periodontal tissue healing in dogs. Seventy-two mandibular premolar roots from ten dogs were extracted and randomly divided into six groups (n = 12 each): (1) positive control group; (2) negative control group; (3) cementum-removed and PACM-treated group; (4) cementum-preserved and PACM-treated group; (5) CPNE7-inactivated PACM-treated group; and (6) recombinant CPNE7-treated group...
March 10, 2018: Journal of Molecular Histology
Renata Nunes Cabral, Jorge Faber, Simone Auxiliadora Morais Otero, Leandro Augusto Hilgert, Soraya Coelho Leal
OBJECTIVES: The aim of this study was to compare the caries-preventive effect and the retention rates of sealants prepared with a new modified and a high-viscosity glass-ionomer cement (GIC) in recently erupted first permanent molars.  MATERIALS AND METHODS: Fifty-six children (224 teeth) were included in a split-mouth randomised clinical trial. All children had their four first permanent molars sealed with either Clinpro XT Varnish (CXT) or Fuji IX GP FAST (FJ). FJ sealants were placed according to the ART protocol...
March 9, 2018: Clinical Oral Investigations
Bingbing An, Dongsheng Zhang
Dentin is a biocomposite possessing complex hierarchical structure, which endows this hard tissue with excellent damage tolerance. In this study, crack growth in dentin at the microstructural scale is investigated and the synergistic effects of plastic deformation of intertubular dentin (ITD), elasticity and fracture properties of peritubular dentin (PTD), and fracture properties of PTD/ITD interface on the fracture of dentin are explored. A micromechanical model is developed, which captures the experimentally observed fracture process of dentin, i...
February 26, 2018: Journal of the Mechanical Behavior of Biomedical Materials
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