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https://www.readbyqxmd.com/read/29346398/dual-function-of-edta-with-silver-nanoparticles-for-root-canal-treatment-a-novel-modification
#1
Juan M Martinez-Andrade, Miguel Avalos-Borja, Alfredo R Vilchis-Nestor, Luis O Sanchez-Vargas, Ernestina Castro-Longoria
The chelating and antimicrobial capacity of a novel modification of 17% EDTA with silver nanoparticles (AgNPs) (EDTA-AgNPs) was evaluated in-vitro for root canal treatment (RCT). The EDTA-AgNPs solution was characterized by UV-Vis spectroscopy, ζ-potential and high-resolution transmission electron microscopy (HRTEM). Antimicrobial capacity was evaluated against Candida albicans and Staphylococcus aureus in planktonic and biofilm cells by broth macrodilution (24 h) and XTT assays, (1, 10 and 30 min) respectively...
2018: PloS One
https://www.readbyqxmd.com/read/29345720/effects-of-nicotine-administration-in-rats-on-mmp2-and-vegf-levels-in-periodontal-membrane
#2
Buşra Deveci, Buket Ayna, İbrahim Halil Tacir, Engin Deveci, Mehmet Cudi Tuncer, Ayşegül Pala
Nicotine is associated with increased incidence of periodontal disease and poor response to therapy. This article aimed at identifying the expression of Matrix metalloproteinases-2 (MMP2) and Vascular endothelial growth factor (VEGF) proteins on extracellular matrix, fibrous distribution and angiogenetic development in periodontitis caused by nicotine effects on periodontal membrane. In this experimental study, the groups were divided into nicotine and control groups. While the nicotine groups in rats (n=6) were administered 2mg/kg Nicotine sulphate for 28 days, the control group in rats (n=6) were only administered 1,5 ml physiologic saline solution subcutaneously for 28 days...
January 18, 2018: Folia Morphologica (Warsz)
https://www.readbyqxmd.com/read/29345716/endodontium-together-or-separately
#3
Adriana Kleinert, Lukas Kleinert, Monika Ozimirska, Renata Chałas
Endodontium, otherwise referred to as pulp-dentin complex or endodont. This term includes two tooth tissues: dentin and pulp, which constitute a structural and functional unity. These tissues have a huge, inseparable influence on each other - the pulp (inter alia) nourishes the dentine, while the dentin forms a protective barrier for the pulp. They develop from the papillary tissue (Latin: papilladentis) from mesenchymal tissue. Nevertheless, in clinical practice this structural-functional complex is often treated as two separate tissues, and not as a whole...
January 18, 2018: Folia Morphologica (Warsz)
https://www.readbyqxmd.com/read/29345388/dentin-bond-strength-and-nanoleakage-of-the-adhesive-interface-after-intracoronal-bleaching
#4
Vanessa Cavalli, Maicon Sebold, Mirela Sanae Shinohara, Patrícia Nóbrega Rodrigues Pereira, Marcelo Giannini
This study evaluated dentin bond strength (BS) and nanoleakage of non- and pre-etched dentin immediately (T0 ,), 7 days (T7 ), and 14 days (T14 ) after bleaching. Bovine incisors (150) were selected and half of them submitted to intrapulpal dentin etching (e). Non- and pre-etched dentin were subjected to the following (n = 15): no bleaching/control (C); 35% carbamide peroxide (CP); 35% hydrogen peroxide (35% HP); 25% hydrogen peroxide (25% HP); and sodium perborate (SP). Bleaching agents were applied to the pulp chamber four times within a 72-h interval...
January 18, 2018: Microscopy Research and Technique
https://www.readbyqxmd.com/read/29344806/comparison-of-two-observational-methods-scanning-electron-and-confocal-laser-scanning-microscopies-in-the-adhesive-interface-analysis-of-endodontic-sealers-to-root-dentine
#5
Maybell Tedesco, Marcelo Carvalho Chain, Eduardo Antunes Bortoluzzi, Lucas da Fonseca Roberti Garcia, Ana Maria Hecke Alves, Cleonice Silveira Teixeira
OBJECTIVES: To compare the accuracy of confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) during the analysis of the adhesive interface integrity and intratubular penetration of root canal sealers to radicular dentine. MATERIALS AND METHODS: Twenty roots of human maxillary incisors were prepared and distributed into two groups (n = 10), followed by filling with gutta-percha and Endofill (G1) or AH Plus (G2). After 7 days, roots were sectioned and analyzed under CLSM and SEM...
January 17, 2018: Clinical Oral Investigations
https://www.readbyqxmd.com/read/29344653/a-novel-variant-of-osteogenesis-imperfecta-type-iv-and-low-serum-phosphorus-level-caused-by-a-val94asp-mutation-in-col1a1
#6
Qi Yang, Hong Xu, Jinsi Luo, Qinle Zhang, Bobo Xie, Sheng Yi, Xiuliang Rong, Jin Wang, Zailong Qin, Tingting Jiang, Li Lin, Yangjin Zuo, Xin Fan
Osteogenesis imperfecta (OI) is a rare congenital disorder characterized by bone fragility and fractures, and associated with bone deformity, short stature, dentin, ligament and blue‑gray eye sclera. OI is caused by a heterozygous mutation in collagen α‑1(I) chain (COL1A1) or collagen α‑2(I) chain (COL1A2) genes that encode α chains of type I collagen. Collagen α chain peptide contains an N‑propeptide, which has a role in assembly and processing of collagen. Point mutations in the N‑propeptide domain appear to trigger OI...
January 16, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29342454/sodium-trimetaphosphate-as-a-novel-strategy-for-matrix-metalloproteinase-inhibition-and-dentin-remineralization
#7
Rafael Simões Gonçalves, Polliana Mendes Candia Scaffa, Marina Ciccone Giacomini, Cristina de Mattos Pimenta Vidal, Heitor Marques Honório, Linda Wang
The effect of sodium trimetaphosphate (STMP) as an antiproteolytic and remineralizing agent on demineralized dentin was evaluated in vitro. The inhibitory potential of STMP at 0.5, 1.5, 3.5, and 5% against recombinant matrix metalloproteinases (MMPs) MMPs-2 and -9 was assessed by zymography. To investigate its remineralization potential, 40 bovine root specimens were obtained and subjected to a demineralization protocol to produce caries-like dentin lesions. After that, dentin surfaces were divided into 3 areas: (1) mineralized (no treatment); (2) demineralized; and (3) demineralized/treated with STMP and submitted to a pH-cycling associated or not with STMP (1...
January 18, 2018: Caries Research
https://www.readbyqxmd.com/read/29342093/hydroxyl-ion-diffusion-through-radicular-dentine-when-calcium-hydroxide-is-used-under-different-conditions
#8
Michael Cai, Paul Abbott, Jacqueline Castro Salgado
Calcium hydroxide's anti-bacterial action relies on high pH. The aim here was to investigate hydroxyl ion diffusion through dentine under different conditions. Teeth were divided into control (n = 4) and four experimental groups (n = 10): Group 1-no medicament; Group 2-Calmix; Group 3-Calmix/Ledermix; Group 4-Calasept Plus/Ledermix; Group 5-Pulpdent/smear layer. Deep (inner dentine) and shallow (outer dentine) cavities were cut into each root. pH was measured in these cavities for 12 weeks. The inner and outer dentine pH in Group 2 was significantly higher than all groups...
January 17, 2018: Materials
https://www.readbyqxmd.com/read/29341381/effect-of-ethanol-on-the-antimicrobial-properties-of-chlorhexidine-over-oral-biofilm
#9
Pedro Henrique Marks Duarte, Paula Barcellos da Silva, Ricardo Abreu da Rosa, Francisco Montagner, Marco Antônio Húngaro Duarte, Milton Carlos Kuga, Marcus Vinícius Reis Só
The aim of this study was to evaluate the effect of 95% ethanol irrigation, with 5 or 10 min of action, on the antibacterial properties of 2% chlorhexidine (CHX), on oral biofilm, evaluated with confocal laser scanning microscopy (CLSM). Oral biofilm development was induced in 80 sterilized bovine dentin blocks, distributed in two groups (5 or 10 min) and 4 subgroups, according to time and the solution used: Saline (SALINE5, SALINE10); Saline followed by CHX (SALINE/CHX5, SALINE/CHX10); Ethanol (ETHANOL5, ETHANOL10), Ethanol followed by CHX (ETHANOL/CHX5, ETHANOL/CHX10)...
January 17, 2018: Microscopy Research and Technique
https://www.readbyqxmd.com/read/29341308/stiffness-regulates-the-proliferation-and-osteogenic-odontogenic-differentiation-of-human-dental-pulp-stem-cells-via-the-wnt-signalling-pathway
#10
Nanxin Liu, Mi Zhou, Qi Zhang, Tao Zhang, Taoran Tian, Quanquan Ma, Changyue Xue, Shiyu Lin, Xiaoxiao Cai
OBJECTIVES: Researches showed that stiffness of the extracellular matrix can affect the differentiation of many stem cells. Dental pulp stem cells (DPSCs) are a promising type of adult stem cell. However, we know little about whether and how the behaviour of DPSCs is influenced by stiffness. MATERIALS AND METHODS: We carried out a study that cultured DPSCs on tunable elasticity polydimethylsiloxane substrates to investigate the influence on morphology, proliferation, osteogenic/odontogenic differentiation and its possible mechanism...
January 17, 2018: Cell Proliferation
https://www.readbyqxmd.com/read/29340524/efficacy-of-removal-of-cariogenic-bacteria-and-carious-dentin-by-ablation-using-different-modes-of-er-yag-lasers
#11
A Baraba, L Kqiku, D Gabrić, Ž Verzak, K Hanscho, I Miletić
The primary objective of this in vitro study was to evaluate the efficiency of removal of cariogenic bacteria and carious dentin by ablation using two lasers: fluorescence-feedback controlled (FFC) Er:YAG laser and different pulses of Er:YAG laser based on variable square pulse technology (VSPt). The secondary objective was to measure the temperature during laser ablation of carious tissue. Seventy-two extracted human molars were used in this study. Sixty teeth with carious dentin were randomly divided into four experimental groups according to the treatment for caries removal: group 1: 400 µs (FFC group); group 2: super short pulse (SSP group, 50 µs pulse); group 3: medium short pulse (MSP group, 100 µs pulse); group 4: short pulse (SP group, 300 µs pulse) and one positive control group with no treatment...
January 11, 2018: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/29340481/evaluation-of-the-sealing-ability-of-different-root-canal-sealers-a-combined-sem-and-micro-ct-study
#12
Yan Huang, Kaan Orhan, Berkan Celikten, Ayşe Işıl Orhan, Pelin Tufenkci, Semra Sevimay
OBJECTIVE: The purpose of this study was to analyze the ability of multiple compounds to seal the dental tubules using scanning electron microscopy (SEM) and micro-computed tomogra-phy (micro-CT). MATERIAL AND METHODS: Twenty-four single-root human mandibular premolars were selected and instrumented with nickel-titanium rotary file and the final file size was #40/06. They were then randomly allocated into 2 groups, and all samples were filled with single cone gutta-percha (#40/06) and one of the tested sealers (AH Plus and EndoSequence BC sealers)...
January 15, 2018: Journal of Applied Oral Science: Revista FOB
https://www.readbyqxmd.com/read/29340355/human-histologic-evidence-of-new-bone-formation-and-osseointegration-between-root-dentin-unplanned-socket-shield-and-dental-implant-case-report
#13
Charles Schwimer, Gregory A Pette, Howard Gluckman, Maurice Salama, Jonathan Du Toit
The socket-shield technique described 7 years ago has since grown in its reporting in the literature as a valid method of ridge preservation at immediate implant placement. To date, large clinical cohorts with up-to-4-year follow-up have been reported. Additionally, evidence of tissue histology at the dental implant and socket-shield has been demonstrated in the animal model. However, human histologic evidence has not yet been available, and the clinician's uncertainty regarding the tissues that may form between the socket-shield and dental implant may remain unanswered until now...
January 2018: International Journal of Oral & Maxillofacial Implants
https://www.readbyqxmd.com/read/29339648/caries-preventive-effect-of-naf-naf-plus-tcp-naf-plus-cpp-acp-and-sdf-varnishes-on-sound-dentin-and-artificial-dentin-caries-in-vitro
#14
Richard J Wierichs, Sabrina Stausberg, Julian Lausch, Hendrik Meyer-Lueckel, Marcella Esteves-Oliveira
The aim of this study was to compare the caries-preventive effect of different fluoride varnishes on sound dentin as well as on artificial dentin caries-like lesions. Bovine dentin specimens (n = 220) with one sound surface (ST) and one artificial caries lesion (DT) were prepared and randomly allocated to 11 groups. The interventions before pH cycling were as follows: application of a varnish containing NaF (22,600 ppm F-; Duraphat [NaF0/NaF1]), NaF plus tricalcium phosphate (22,600 ppm F-; Clinpro White Varnish Mint [TCP0/TCP1]), NaF plus casein phosphopeptide-stabilized amorphous calcium phosphate complexes (CPP-ACP; 22,600 ppm F-; MI Varnish [CPP0/CPP1]), or silver diamine fluoride (SDF; 35,400 ppm F-; Cariestop 30% [SDF0/SDF1]) and no intervention (NNB/N0/N1)...
January 17, 2018: Caries Research
https://www.readbyqxmd.com/read/29338011/validation-of-growth-layer-group-glg-depositional-rate-using-daily-incremental-growth-lines-in-the-dentin-of-beluga-delphinapterus-leucas-pallas-1776-teeth
#15
David A Waugh, Robert S Suydam, Joseph D Ortiz, J G M Thewissen
Counts of Growth Layer Groups (GLGs) in the dentin of marine mammal teeth are widely used as indicators of age. In most marine mammals, observations document that GLGs are deposited yearly, but in beluga whales, some studies have supported the view that two GLGs are deposited each year. Our understanding of beluga life-history differs substantially depending on assumptions regarding the timing of GLG deposition; therefore, resolving this issue has important considerations for population assessments. In this study, we used incremental lines that represent daily pulses of dentin mineralization to test the hypothesis that GLGs in beluga dentin are deposited on a yearly basis...
2018: PloS One
https://www.readbyqxmd.com/read/29337188/abrogation-of-fam20c-altered-cell-behaviors-and-bmp-signaling-of-immortalized-dental-mesenchymal-cells
#16
Chao Liu, Nan Zhou, Yu Wang, Hua Zhang, Priyam Jani, Xiaofang Wang, Yongbo Lu, Nan Li, Jing Xiao, Chunlin Qin
FAM20C mutations compromise the mineralization of skeleton and tooth in both human and mouse. Putatively, the mineralization disorder is attributed to the elevated fibroblast growth factor 23 (FGF23), which reduced the serum phosphorus by suppressing the reabsorption of phosphorus in kidney. Besides the regulation on systemic phosphorus homeostasis, FAM20C was also implicated to regulate cell behaviors and gene expression through a cell-autonomous manner. To identify the primary effects of Fam20c on dental mesenchymal cells, mouse Fam20c-deficient dental mesenchymal cells were generated by removing the floxed alleles from the immortalized mouse Fam20cf/f dental mesenchymal cells with Cre-expressing lentivirus...
January 11, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29336881/influence-of-contracted-endodontic-access-on-root-canal-geometry-an-in%C3%A2-vitro-study
#17
Mario Alovisi, Damiano Pasqualini, Edoardo Musso, Elena Bobbio, Carlotta Giuliano, Davide Mancino, Nicola Scotti, Elio Berutti
INTRODUCTION: Contracted endodontic cavities (CECs) have developed from the concept of minimally invasive dentistry and provide an alternative to traditional endodontic cavities (TECs). They have been designed in an effort to preserve the mechanical stability of teeth. The contracted cavity design preserves more of the dentin but may influence the geometric shaping parameters. The aim of this micro-computed tomographic study was to evaluate the influence of contracted endodontic cavities on the preservation of the original root canal anatomy after shaping with nickel-titanium rotary instruments...
January 12, 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29336880/marginal-gaps-between-2-calcium-silicate-and-glass-ionomer-cements-and-apical-root-dentin
#18
Vladimir Biočanin, Đorđe Antonijević, Srđan Poštić, Dragan Ilić, Zorica Vuković, Marija Milić, Yifang Fan, Zhiyu Li, Božidar Brković, Marija Đurić
INTRODUCTION: The outcome of periapical surgery has been directly improved with the introduction of novel material formulations. The aim of the study was to compare the retrograde obturation quality of the following materials: calcium silicate (Biodentine; Septodont, Saint-Maur-des-Fosses, France), mineral trioxide aggregate (MTA+; Cerkamed Company, Stalowa Wola, Poland), and glass ionomer cement (Fuji IX; GC Corporation, Tokyo, Japan). METHODS: Materials' wettability was calculated concerning the contact angles of the cements measured using a glycerol drop...
January 12, 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29335687/structure-of-the-bacterial-community-in-different-stages-of-early-childhood-caries
#19
Marcos Ximenes, Rafael Dutra de Armas, Thaisa Cezária Triches, Mariane Cardoso, Ricardo de Souza Vieira
PURPOSE: To characterise in vivo the structure of bacterial communities in decayed and sound primary teeth. MATERIALS AND METHODS: Samples of biofilms were collected from three groups of patients with complete and exclusively primary dentition (n = 45): G1: sound teeth (n = 15); G2: enamel lesion (n = 15); G3: dentin lesion (n = 15). DNA was extracted (CTAB 2%) from the biofilm, the partial 16S rRNA gene was amplified with Bacteria Universal Primers (BA338fGC - UN518r) and subjected to DGGE (denaturing gradient gel electrophoresis)...
January 15, 2018: Oral Health & Preventive Dentistry
https://www.readbyqxmd.com/read/29335686/synthetic-hydroxyapatite-as-a-biomimetic-oral-care-agent
#20
Joachim Enax, Matthias Epple
PURPOSE: Human tooth enamel consists mostly of minerals, primarily hydroxyapatite, Ca10(PO4)6(OH)2, and thus synthetic hydroxyapatite can be used as a biomimetic oral care agent. This review describes the synthesis and characterization of hydroxyapatite from a chemist's perspective and provides an overview of its current use in oral care, with a focus on dentin hypersensitivity, caries, biofilm management, erosion, and enamel lesions. SOURCES: Reviews and original research papers published in English and German were included...
January 15, 2018: Oral Health & Preventive Dentistry
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