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https://www.readbyqxmd.com/read/28231619/connective-tissue-graft-and-tunnel-or-trapezoidal-flap-for-the-treatment-of-single-maxillary-gingival-recessions-a-randomized-clinical-trial
#1
Mauro Pedrine Santamaria, Felipe Lucas da Silva Neves, Camila Augusto Silveira, Ingrid Fernandes Mathias, Stephanie Botti Fernandes-Dias, Maria Aparecida Neves Jardini, Dimitris N Tatakis
BACKGROUND: Although ample evidence supports connective tissue graft (CTG) use for root coverage, there is need for research on recipient site preparation approaches. The purpose of this study was to compare the outcomes of trapezoidal coronally advanced flap (CAF) and coronally advanced tunnel flap (TUN) when used in conjunction with CTG. METHODS: Forty-two patients presenting 42 single maxillary, Miller Class I and II, gingival recession defects were randomly assigned to receive either CAF+CTG (N=21) or TUN+CTG (N=21)...
February 23, 2017: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/28229586/chlorhexidine-loaded-amorphous-calcium-phosphate-nanoparticles-for-inhibiting-degradation-and-inducing-mineralization-of-type-i-collagen
#2
Xue Cai, Bing Han, Yan Liu, Fucong Tian, Fuxin Liang, Xiaoyan Wang
A major shortcoming of contemporary dentin adhesives is their limited durability. Exposed collagen fibrils within the bonding interface are degraded by matrix metalloproteinases (MMPs), resulting in aging of the resin-dentin bond. In this study, chlorhexidine-loaded amorphous calcium phosphate (ACP) nanoparticles were synthesized to induce the mineralization of collagen fibrils. The nanoparticles sustainably released chlorhexidine, to inhibit MMPs during mineralization. Three types of ACP nanoparticles were prepared: N-ACP containing no chlorhexidine, C-ACP containing chlorhexidine acetate, and G-ACP containing chlorhexidine gluconate, which had a higher drug-loading than C-ACP...
February 23, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28229417/a-simplified-and-systematic-method-to-isolate-culture-and-characterize-multiple-types-of-human-dental-stem-cells-from-a-single-tooth
#3
Mohammed Bakkar, Younan Liu, Dongdong Fang, Camille Stegen, Xinyun Su, Murali Ramamoorthi, Li-Chieh Lin, Takako Kawasaki, Nicholas Makhoul, Huan Pham, Yoshinori Sumita, Simon D Tran
This chapter describes a simplified method that allows the systematic isolation of multiple types of dental stem cells such as dental pulp stem cells (DPSC), periodontal ligament stem cells (PDLSC), and stem cells of the apical papilla (SCAP) from a single tooth. Of specific interest is the modified laboratory approach to harvest/retrieve the dental pulp tissue by minimizing trauma to DPSC by continuous irrigation, reduction of frictional heat from the bur rotation, and reduction of the bur contact time with the dentin...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28228165/determining-optimal-fluorescent-agent-concentrations-in-dental-adhesive-resins-for-imaging-the-tooth-restoration-interface
#4
Odair Bim Júnior, Marco A Cebim, Maria T Atta, Camila M Machado, Luciana F Francisconi-Dos-Rios, Linda Wang
Fluorescent dyes like Rhodamine B (RB) have been used to identify the spatial distribution of adhesive restorative materials in the tooth/restoration interface. Potential effects of the addition of RB to dental adhesives were addressed in the past, but no further information is available on how to determine suitable concentrations of RB in these bonding agents for imaging in the confocal laser scanning microscope. This study provides systematical strategies for adding RB to viscous dental adhesive resins, focusing on the determination of the lowest range of dye concentrations necessary to achieve an acceptable image of the dentin/adhesive interface...
February 2017: Microscopy and Microanalysis
https://www.readbyqxmd.com/read/28223198/novel-bioactive-root-canal-sealer-to-inhibit-endodontic-multispecies-biofilms-with-remineralizing-calcium-phosphate-ions
#5
Lin Wang, Xianju Xie, Chunyan Li, Huaibing Liu, Ke Zhang, Yanmin Zhou, Xiaofeng Chang, Hockin H K Xu
OBJECTIVE: The objectives of this study were to: (1) develop a bioactive endodontic sealer via dimethylaminohexadecyl methacrylate (DMAHDM), 2-methacryloyloxyethyl phosphorylcholine (MPC) and nanoparticles of amorphous calcium phosphate (NACP) for the first time; and (2) evaluate inhibition of early-stage and mature multispecies endodontic biofilm, bond strength to root canal dentin, and calcium (Ca) and phosphate (P) ion release. METHODS: A series of bioactive endodontic sealers were formulated with DMAHDM, MPC, and NACP...
February 18, 2017: Journal of Dentistry
https://www.readbyqxmd.com/read/28222907/fatigue-testing-of-biomaterials-and-their-interfaces
#6
Dwayne Arola
OBJECTIVE: The objective of this article is to describe the importance of fatigue to the success of restorative dentistry, with emphasis on the methods for evaluating the fatigue properties of materials in this field, and the durability of their bonded interfaces. METHODS: The stress-life fatigue and fatigue crack growth approaches for evaluating the fatigue resistance of dental biomaterials are introduced. Emphasis is placed on in vitro studies of the hard tissue foundation, restorative materials and their bonded interfaces...
February 17, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/28222875/comparison-of-push-out-bond-strength-of-fiber-reinforced-composite-resin-posts-according-to-cement-thickness
#7
Jun-Seong Park, Jeong-Sub Lee, Jeong-Won Park, Won-Gyun Chung, Eun-Hee Choi, Yoon Lee
STATEMENT OF PROBLEM: Post space size and cement thickness can differ because of variations in root canal morphology, such as an oval shape, and because the entire canal space cannot be included in the post space preparation. As a result, increased cement thickness around the post may affect the bond strength between the post and the dentin. PURPOSE: The purpose of this in vitro study was to evaluate the push-out bond strength of fiber-reinforced composite resin posts to root dentin with cement layers of varying thickness...
February 17, 2017: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/28222429/effect-of-5-000-ppm-fluoride-dentifrice-or-1-100-ppm-fluoride-dentifrice-combined-with-acidulated-phosphate-fluoride-on-caries-lesion-inhibition-and-repair
#8
Constanza E Fernández, Livia Maria Andaló Tenuta, Altair Antoninha Del Bel Cury, Diego Figueiredo Nóbrega, Jaime Aparecido Cury
High fluoride dentifrice (FD; 5,000 ppm F) has been recommended to arrest root dentine lesions and to control enamel caries in high-risk patients. Also, standard FD (1,100 ppm F) in combination with professional fluoride application has been recommended to control dentine caries, but the effect of this combination on enamel has been considered modest. Considering the lack of evaluation comparing the use of 5,000 ppm FD (5,000-FD) versus acidulated phosphate fluoride (APF) application combined with 1,100 ppm FD (1,100-FD) on the inhibition and repair of caries lesions in both enamel and dentine, we conducted this in situ, double-blind, crossover study of 3 phases of 14 days...
February 22, 2017: Caries Research
https://www.readbyqxmd.com/read/28222209/effect-of-different-intermediary-bases-on-microleakage-of-a-restorative-material-in-class-ii-box-cavities-of-primary-teeth
#9
Faika Y Abdelmegid, Fouad S Salama, Waleed M Al-Mutairi, Saud K Al-Mutairi, Sultan O Baghazal
INTRODUCTION: The aim of this in vitro study was to assess and compare the effect of different intermediary bases on microleakage between tooth and a nanocomposite interface in Class II box cavities in primary teeth. METHODS: Standard Class II box cavities were prepared in 52 primary molars and randomly divided into 9 groups according to the intermediary base used (Multicore Flow, Fuji II LC, SDR, Smart Dentin Replacement, and Biodentine). All specimens were subjected to thermocycling and prepared for microleakage testing and evaluation...
February 21, 2017: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/28222156/toothbrush-abrasivity-in-a-long-term-simulation-on-human-dentin-depends-on-brushing-mode-and-bristle-arrangement
#10
Mozhgan Bizhang, Ilka Schmidt, Yong-Hee Patricia Chun, Wolfgang H Arnold, Stefan Zimmer
OBJECTIVE: The aim of this study was to evaluate the susceptibility of dentin to brushing abrasion using four different toothbrushes (rotating-oscillating, sonic and two types of manual toothbrushes) with the same brushing forces. METHODS: Dentin samples (n = 72) were selected from 72 impacted third molars. Half of the surface of dentin samples was covered with an adhesive tape, creating a protected and a freely exposed area in the same specimen. Brushing was performed with either a: sonic (Sonicare PowerUp, Philips GmbH, Hamburg, Germany), b: oscillating-rotating (Oral B Vitality Precisions Clean, Procter & Gamble, Schwalbach am Taunus, Germany) or two different manual toothbrushes c: flat trim brush head toothbrush (Dr...
2017: PloS One
https://www.readbyqxmd.com/read/28222099/morphological-study-of-tooth-development-in-podoplanin-deficient-mice
#11
Kenyo Takara, Naoki Maruo, Kyoko Oka, Chiaki Kaji, Yuji Hatakeyama, Naruhiko Sawa, Yukinari Kato, Junro Yamashita, Hiroshi Kojima, Yoshihiko Sawa
Podoplanin is a mucin-type highly O-glycosylated glycoprotein identified in several somatyic cells: podocytes, alveolar epithelial cells, lymphatic endothelial cells, lymph node stromal fibroblastic reticular cells, osteocytes, odontoblasts, mesothelial cells, glia cells, and others. It has been reported that podoplanin-RhoA interaction induces cytoskeleton relaxation and cell process stretching in fibroblastic cells and osteocytes, and that podoplanin plays a critical role in type I alveolar cell differentiation...
2017: PloS One
https://www.readbyqxmd.com/read/28219674/exploratory-randomised-controlled-clinical-study-to-evaluate-the-comparative-efficacy-of-two-occluding-toothpastes-a-5-calcium-sodium-phosphosilicate-toothpaste-and-an-8-arginine-calcium-carbonate-toothpaste-for-the-longer-term-relief-of-dentine-hypersensitivity
#12
Claire Hall, Stephen Mason, Jonathan Cooke
OBJECTIVE: To compare the longer-term clinical efficacy of two occlusion-technology toothpastes - a 5% calcium sodium phosphosilicate (CSPS) toothpaste and a commercially available 8% arginine/calcium carbonate toothpaste - in relieving dentine hypersensitivity (DH). Efficacy was also compared with that of a regular fluoride toothpaste control. METHODS: This was an exploratory, randomised, examiner-blind, parallel-group, 11-week, controlled study in healthy adults with self-reported and clinically diagnosed DH...
February 17, 2017: Journal of Dentistry
https://www.readbyqxmd.com/read/28219056/anticaries-potential-of-a-sodium-monofluorophosphate-dentifrice-containing-calcium-sodium-phosphosilicate-exploratory-in-situ-randomized-trial
#13
Charles R Parkinson, Muhammad Siddiqi, Stephen Mason, Frank Lippert, Anderson T Hara, Domenick T Zero
Calcium sodium phosphosilicate (CSPS) is a bioactive glass material that alleviates dentin hypersensitivity and is postulated to confer remineralization of caries lesions. This single-centre, randomized, single (investigator)-blind, placebo-controlled, crossover, in situ study explored whether the addition of 5% CSPS to a nonaqueous fluoride (F) such as sodium monofluorophosphate (SMFP)-containing dentifrice affects its cariostatic ability. Seventy-seven subjects wore 4 gauze-covered enamel specimens with preformed lesions (2 surface-softened and 2 subsurface) placed buccally on their mandibular bilateral dentures for up to 4 weeks...
February 21, 2017: Caries Research
https://www.readbyqxmd.com/read/28216274/micro-computed-tomography-assessment-of-dentinal-micro-cracks-after-root-canal-preparation-with-trushape-and-self-adjusting-file-systems
#14
Mario Luis Zuolo, Gustavo De-Deus, Felipe Gonçalves Belladonna, Emmanuel João Nogueira Leal da Silva, Ricardo Tadeu Lopes, Erick Miranda Souza, Marco Aurélio Versiani, Alexandre Augusto Zaia
INTRODUCTION: The aim of the present study was to evaluate the percentage frequency of dentinal micro-cracks observed after root canal preparation with TRUShape and Self-Adjusting File (SAF) systems by means of micro-computed tomography imaging analysis. A conventional full-sequence rotary system (BioRace) and a single-file reciprocation system (Reciproc) were used as reference techniques for comparison because of their known assertive cutting efficiency. METHODS: Forty anatomically matched mandibular incisors were selected, scanned at a resolution of 14...
February 16, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28216271/comparative-analysis-of-2-calcium-silicate-based-cements-biodentine-and-mineral-trioxide-aggregate-as-direct-pulp-capping-agent-in-young-permanent-molars-a-split-mouth-study
#15
Farhin A Katge, Devendra P Patil
INTRODUCTION: The purpose of this study was to compare Biodentine and mineral trioxide aggregate (MTA) for direct pulp capping in young permanent molars by clinical and radiographic evaluation in 7- to 9-year-old children. METHODS: In 50 patients, 29 patients with bilateral asymptomatic first permanent molars with carious involvement were selected. According to split mouth design, these patients were then divided into 2 groups, Biodentine group (right side) and MTA group (left side)...
February 16, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28216269/antibacterial-effects-of-antimicrobials-used-in-regenerative-endodontics-against-biofilm-bacteria-obtained-from-mature-and-immature-teeth-with-necrotic-pulps
#16
Jordon C Jacobs, Alex Troxel, Ygal Ehrlich, Kenneth Spolnik, Josef S Bringas, Richard L Gregory, Ghaeth H Yassen
INTRODUCTION: We investigated the direct and residual antibacterial effects of intracanal antimicrobials against bacterial biofilms obtained from infected mature and immature teeth with necrotic pulps. METHODS: Sterile dentin slabs (n = 100) were inoculated with bacterial biofilms obtained from root canals of an immature or a mature tooth with pulpal necrosis and incubated anaerobically for 3 weeks (n = 50 per biofilm). Dentin infected with each type of biofilm received 1 week of treatment with 1 or 5 mg/mL double antibiotic paste (DAP) in methylcellulose hydrogels, calcium hydroxide, or placebo paste or received no treatment (n = 10)...
February 16, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28216268/dental-pulp-tissue-regeneration-using-dental-pulp-stem-cells-isolated-and-expanded-in-human-serum
#17
Evandro Piva, Susan A Tarlé, Jacques E Nör, Duohong Zou, Elizabeth Hatfield, Tyler Guinn, Emily J Eubanks, Darnell Kaigler
INTRODUCTION: Dental pulp-derived stem cells (DPSCs) have the potential to regenerate dentin and dental pulp tissue because of their differentiation capacity and angiogenic properties. However, for regenerative approaches to gain regulatory and clinical acceptance, protocols are needed to determine more feasible ways to cultivate DPSCs, namely, without the use of xenogeneic-derived components (animal sera) and exogenous growth factors. METHODS: In this study, human DPSCs were isolated from third molars and expanded in standard culture conditions containing fetal bovine serum (DPSCs-FBS) or conditions containing human serum (DPSCs-HS)...
February 16, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28216267/dentinal-tubule-penetration-of-a-calcium-silicate-based-root-canal-sealer-with-different-obturation-methods
#18
Ji Wook Jeong, Anna DeGraft-Johnson, Samuel O Dorn, Peter M Di Fiore
INTRODUCTION: The purpose of this study was to investigate the depths of penetration of a calcium silicate-based sealer in dentinal tubules by using 3 different obturation methods. METHODS: One hundred extracted human permanent anterior teeth were endodontically prepared and divided equally into 3 experimental groups and 1 control group as follows: CPoint single cone (CPSC), gutta-percha single cone (GPSC), gutta-percha vertical condensation (GPVC), all with a calcium silicate-based sealer and calcium indicator Fluo-3, and CPoint single cone with a calcium indicator Fluo-3 (CPF3) without sealer as the control...
February 16, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28215628/symmetric-multiquadrant-isolated-dentin-dysplasia-smidd-a-unique-presentation-mimicking-dentin-dysplasia-type-1b
#19
Hiba Qari, Harvey Kessler, Nagamani Narayana, Sundaralingam Premaraj
Dentin dysplasia (DD) is a rare developmental dentin disorder that causes root malformation. It is divided into radicular DD type 1 (DD-1) and coronal DD type 2 (DD-2). Recently, a new entity causing localized root malformation of permanent first molars that resembles DD-1b has been described as molar-incisor malformation (MIM). We report and compare 4 new cases that exhibit similar clinical, histologic, and radiographic features to the new entity, MIM. We believe MIM and our 4 cases to be the same entity, which is nonhereditary and, because of the isolated but bilaterally symmetric pattern of involvement, may be caused by a short-duration environmental insult that disrupts normal development/function of Hertwig's epithelial root sheath...
December 7, 2016: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
https://www.readbyqxmd.com/read/28215344/rac1-regulates-the-proliferation-adhesion-migration-and-differentiation-of-mdpc-23-cells
#20
Jing Ren, Guobin Liang, Li Gong, Bing Guo, Hongwei Jiang
INTRODUCTION: Stem cells are responsible for replacing damaged pulp tissue; therefore, promoting their survival and inducing their adhesion to dentin are vital. As a member of the Rho family of guanosine triphosphatases, Rac1 is an important regulator of osteoblast functions. However, little is known about its role in regenerative endodontic procedures. The current study examined the role of Rac1 in the proliferation, migration, and odontoblastic differentiation of MDPC-23 cells. METHODS: MDPC-23 cells were transfected with small interfering RNA to knock down Rac1 expression, and then their proliferation, migration, adhesion, and odontoblastic differentiation were examined in vitro...
February 15, 2017: Journal of Endodontics
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