Wei-Jiun Tsai, Yi-Hsin Lai, Yong-An Shi, Michal Hammel, Anthony P Duff, Andrew E Whitten, Karyn L Wilde, Chun-Ming Wu, Robert Knott, U-Ser Jeng, Chia-Yu Kang, Chih-Yu Hsu, Jian-Li Wu, Pei-Jane Tsai, Chuan Chiang-Ni, Jiunn-Jong Wu, Yee-Shin Lin, Ching-Chuan Liu, Toshiya Senda, Shuying Wang
Group A Streptococcus (GAS) is a strict human pathogen possessing a unique pathogenic trait that utilizes the cooperative activity of NAD+ -glycohydrolase (NADase) and Streptolysin O (SLO) to enhance its virulence. How NADase interacts with SLO to synergistically promote GAS cytotoxicity and intracellular survival is a long-standing question. Here, the structure and dynamic nature of the NADase/SLO complex are elucidated by X-ray crystallography and small-angle scattering, illustrating atomic details of the complex interface and functionally relevant conformations...
January 31, 2023: Communications Biology