keyword
MENU ▼
Read by QxMD icon Read
search

Bioprospecting

keyword
https://www.readbyqxmd.com/read/28058469/fungal-endophytes-an-alternative-source-for-production-of-volatile-compounds-from-agarwood-oil-of-aquilaria-subintegra
#1
Sakon Monggoot, Siam Popluechai, Eleni Gentekaki, Patcharee Pripdeevech
Fungal endophytes are microorganisms that are well-known for producing a diverse array of secondary metabolites. Recent studies have uncovered the bioprospecting potential of several plant endophytic fungi. Here, we demonstrate the presence of highly bioactive fungal endophytic species in Aquilaria subintegra, a fragrant wood plant collected from Thailand. Thirty-three fungal endophytic strains were isolated and further identified to genus level based on morphological characteristics. These genera included Colletotrichum, Pestalotiopsis, Fusarium, Russula, Arthrinium, Diaporthe and Cladosporium...
January 5, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28044276/antimicrobial-potential-and-taxonomic-investigation-of-piezotolerant-streptomyces-sp-niot-ch-40-isolated-from-deep-sea-sediment
#2
Vishnu Priya Padmanaban, Pankaj Verma, Srividhyalakshmi Venkatabaskaran, Thirupathi Keppayan, Dharani Gopal, Ashok Kumar Sekar, Kirubagaran Ramalingam
Microbial-derived natural products from extreme niches such as deepsea are known to possess structural and functional novelty. With this background, the present study was designed to investigate the bioprospecting potential and systematics of a deep-sea derived piezotolerant bacterial strain NIOT-Ch-40, showing affiliation to the genus Streptomyces based on 16S RNA gene similarity. Preliminary screening for the presence of biosynthetic genes like polyketide synthase I, polyketide synthase II, non ribosomal peptide synthase, 3-amino-5-hydroxybenzoic acid synthase and spiroindimicin followed by antibacterial activity testing confirmed the presence of potent bioactivity...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27984002/endophytes-as-sources-of-antibiotics
#3
REVIEW
Elena Martinez-Klimova, Karol Rodríguez-Peña, Sergio Sánchez
Until a viable alternative can be accessible, the emergence of resistance to antimicrobials requires the constant development of new antibiotics. Recent scientific efforts have been aimed at the bioprospecting of microorganisms' secondary metabolites, with special emphasis on the search for antimicrobial natural products derived from endophytes. Endophytes are microorganisms that inhabit the internal tissues of plants without causing apparent harm to the plant. The present review article compiles recent (2006-2016) literature to provide an update on endophyte research aimed at finding metabolites with antibiotic activities...
October 27, 2016: Biochemical Pharmacology
https://www.readbyqxmd.com/read/27975993/bioprospecting-of-cave-bacteria-with-antifungal-activity
#4
A C Almeida, P F Azevedo, R D Marques, Ect Mello-Peixoto, L S Matsumoto
No abstract text is available yet for this article.
December 2016: Planta Medica
https://www.readbyqxmd.com/read/27975387/phylogenetics-of-psychoactive-plants-in-neuro-targeted-bioprospecting-24852989
#5
M H Gramkow, M Ernst, R R Dunn, C H Saslis-Lagoudakis
No abstract text is available yet for this article.
December 2016: Planta Medica
https://www.readbyqxmd.com/read/27936316/bioprospecting-of-turbinaria-macroalgae-as-a-potential-source-of-health-protective-compounds
#6
Milena Stranska-Zachariasova, Isma Kurniatanty, Helena Gbelcova, Monika Jiru, Josep Rubert, Tjokorda Gde Tirta Nindhia, Cosimo Walter D'Acunto, Sony Heru Sumarsono, Marselina Irasonia Tan, Jana Hajslova, Tomas Ruml
The present study aims to focus on the bioprospecting of marine macroalgae of Turbinaria species, plenteous biomass of the world ocean. Three types of solvents, i.e. water, methanol:water (80:20, v/v) and hexane:isopropanol (50:50, v/v), were used for extraction. Both the biological activity, as well as the pattern of present chemicals were characterized. For the cell proliferation assay, the human embryonic kidney 293 cells, cervix / breast / pancreatic adenocarcinoma, and osteosarcoma cells were used. For the antioxidant activity determination, both intracellular assay with human embryonic kidney and cervix adenocarcinoma cells, as well as the biochemical DPPH test, were employed...
December 9, 2016: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/27931827/selection-of-an-endogenous-2-3-butanediol-pathway-in-escherichia-coli-by-fermentative-redox-balance
#7
Keming Liang, Claire R Shen
Fermentative redox balance has long been utilized as a metabolic evolution platform to improve efficiency of NADH-dependent pathways. However, such system relies on the complete recycling of NADH and may become limited when the target pathway results in excess NADH stoichiometrically. In this study, endogenous capability of Escherichia coli for 2,3-butanediol (2,3-BD) synthesis was explored using the anaerobic selection platform based on redox balance. To address the issue of NADH excess associated with the 2,3-BD pathway, we devised a substrate-decoupled system where a pathway intermediate is externally supplied in addition to the carbon source to decouple NADH recycling ratio from the intrinsic pathway stoichiometry...
December 6, 2016: Metabolic Engineering
https://www.readbyqxmd.com/read/27914891/antioxidant-capacities-of-fucoxanthin-producing-algae-as-influenced-by-their-carotenoid-and-phenolic-contents
#8
Su Chern Foo, Fatimah Md Yusoff, Maznah Ismail, Mahiran Basri, Sook Kun Yau, Nicholas M H Khong, Kim Wei Chan, Mahdi Ebrahimi
Natural antioxidants from sustainable sources are favoured to accommodate worldwide antioxidant demand. In addition to bioprospecting for natural and sustainable antioxidant sources, this study aimed to investigate the relationship between the bioactives (i.e. carotenoid and phenolic acids) and the antioxidant capacities in fucoxanthin-producing algae. Total carotenoid, phenolic acid, fucoxanthin contents and fatty acid profile of six species of algae (five microalgae and one macroalga) were quantified followed by bioactivity evaluation using four antioxidant assays...
January 10, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/27900683/constructing-and-screening-a-metagenomic-library-of-a-cold-and-alkaline-extreme-environment
#9
Mikkel A Glaring, Jan K Vester, Peter Stougaard
Natural cold or alkaline environments are common on Earth. A rare combination of these two extremes is found in the permanently cold (less than 6 °C) and alkaline (pH above 10) ikaite columns in the Ikka Fjord in Southern Greenland. Bioprospecting efforts have established the ikaite columns as a source of bacteria and enzymes adapted to these conditions. They have also highlighted the limitations of cultivation-based methods in this extreme environment and metagenomic approaches may provide access to novel extremophilic enzymes from the uncultured majority of bacteria...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27896615/bioprospecting-hot-spring-metagenome-lipase-for-the-production-of-biodiesel
#10
Rajesh Kumar Sahoo, Mohit Kumar, Lala Behari Sukla, Enketeswara Subudhi
Screening of metagenomic library from Taptapani Hot Spring (Odisha) yielded a positive lipase clone (pUC-lip479). Sequence analysis showed an ORF (RK-lip479) of 416 amino acid residues which was overexpressed in Escherichia coli BL21 (DE3). Optimum pH and temperature of purified lipase RK-lip479 were 8.0 and 65 °C, respectively, and found to be stable over a pH range of 7.0-9.0 and temperatures 55-75 °C. RK-lip479 could hydrolyse a wide range of 4-nitrophenyl esters (4-nitrophenyoctanoate, 4-nitrophenyldodecanoate, 4-nitrophenylpalmitate, 4-nitrophenylmyristate and 4-nitrophenylstearate), and maximum activity was observed with 4-nitrophenyldodecanoate...
November 28, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27890726/sources-of-antibiotics-hot-springs
#11
REVIEW
Girish B Mahajan, Lakshmi Balachandran
The discovery of antibiotics heralded an era of improved health care. However, the over-prescription and misuse of antibiotics resulted in the development of resistant strains of various pathogens. Since then, there has been an incessant search for discovering novel compounds from bacteria at various locations with extreme conditions. The soil is one of the most explored locations for bioprospecting. In recent times, hypersaline environments and symbiotic associations have been investigated for novel antimicrobial compounds...
November 24, 2016: Biochemical Pharmacology
https://www.readbyqxmd.com/read/27866010/the-botanical-explorer-s-legacy-a-promising-bioprospecting-tool
#12
Axel Helmstädter
Records about the traditional uses of medicinal plants can be considered useful in bioprospecting (i.e., the search for new active agents or lead structures in nature). Several sources like Egyptian papyri, early modern herbals and pharmacopoeias have been studied in this respect. It is proposed to use recordings of botanically interested explorers of the 19th and early 20th centuries as well. Some of them give detailed information about traditionally used medicinal plants and analysis shows that a considerable number of these have never been scientifically investigated...
November 16, 2016: Drug Discovery Today
https://www.readbyqxmd.com/read/27856231/bioprospecting-thermotolerant-ethanologenic-yeasts-for-simultaneous-saccharification-and-fermentation-from-diverse-environments
#13
Jairam Choudhary, Surender Singh, Lata Nain
Lignocellulosic biomass, a promising renewable energy source, can be used for the production of second generation bioethanol. Simultaneous saccharification and fermentation (SSF), the process which alleviates the problem of separate hydrolysis and fermentation (SHF), requires thermotolerant ethanologenic yeast for bioethanol production. Therefore, ten yeast strains isolated from diverse sources, belonging to various genera like Saccharomyces, Candida, Pichia and Wickerhamomyces were evaluated for their thermotolerance, sugar utilization pattern, inhibitor tolerance and ethanol production potential with glucose, xylose and alkali pretreated paddy straw...
November 14, 2016: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/27844243/analysis-of-chemical-constituents-antimicrobial-and-anticancer-activities-of-dichloromethane-extracts-of-sordariomycetes-sp-endophytic-fungi-isolated-from-strobilanthes-crispus
#14
Elaine Chin Jinfeng, Mohamed Ikhtifar Mohamad Rafi, Khoo Chai Hoon, Ho Kok Lian, Cheah Yoke Kqueen
Plants are primary source of natural product drugs. However, with every new bioactive molecule reported from a plant source, there follows reports of endangered status or even extinction of a medicinally important plant due to over-harvesting. Hence, the attention turned towards fungi namely the endophytes, which reside within medicinally important plants and thus may have acquired their medicinal properties. Strobilanthes crispus is a traditional medicinal plant which has been used traditionally to treat kidney stones, diabetes, hypertension and cancer as well as having antimicrobial activities...
January 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27822535/an-integrated-metabolomic-and-genomic-mining-workflow-to-uncover-the-biosynthetic-potential-of-bacteria
#15
Maria Maansson, Nikolaj G Vynne, Andreas Klitgaard, Jane L Nybo, Jette Melchiorsen, Don D Nguyen, Laura M Sanchez, Nadine Ziemert, Pieter C Dorrestein, Mikael R Andersen, Lone Gram
Microorganisms are a rich source of bioactives; however, chemical identification is a major bottleneck. Strategies that can prioritize the most prolific microbial strains and novel compounds are of great interest. Here, we present an integrated approach to evaluate the biosynthetic richness in bacteria and mine the associated chemical diversity. Thirteen strains closely related to Pseudoalteromonas luteoviolacea isolated from all over the Earth were analyzed using an untargeted metabolomics strategy, and metabolomic profiles were correlated with whole-genome sequences of the strains...
May 2016: MSystems
https://www.readbyqxmd.com/read/27774089/the-madeira-archipelago-as-a-significant-source-of-marine-derived-actinomycete-diversity-with-anticancer-and-antimicrobial-potential
#16
Alejandra Prieto-Davó, Tiago Dias, Sofia E Gomes, Sara Rodrigues, Yessica Parera-Valadez, Pedro M Borralho, Florbela Pereira, Cecilia M P Rodrigues, Ilda Santos-Sanches, Susana P Gaudêncio
Marine-derived actinomycetes have demonstrated an ability to produce novel compounds with medically relevant biological activity. Studying the diversity and biogeographical patterns of marine actinomycetes offers an opportunity to identify genera that are under environmental pressures, which may drive adaptations that yield specific biosynthetic capabilities. The present study describes research efforts to explore regions of the Atlantic Ocean, specifically around the Madeira Archipelago, where knowledge of the indigenous actinomycete diversity is scarce...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27770865/bioprospects-of-microbial-enzymes-from-mangrove-associated-fungi-and-bacteria
#17
K Saravanakumar, N Rajendran, K Kathiresan, J Chen
Mangroves ecosystem provides the unique habitat for the colonization of fungi and bacteria. Interestingly, the enzymes derived from mangroves associated microorganisms have enormous economic value in industries of agriculture, pulp, paper, medicine, sewage treatments, etc. Microbial enzyme activity is required for the metabolism of plants and animals. In addition, the enzymes are also involved in aquatic animal food cycle and degradation of mangroves detritus. However, the understanding of current status of mangroves associated microorganism-derived enzymes and its application is required to improve the future omics studies...
2016: Advances in Food and Nutrition Research
https://www.readbyqxmd.com/read/27767051/isolation-of-a-euryhaline-microalgal-strain-tetraselmis-sp-ctp4-as-a-robust-feedstock-for-biodiesel-production
#18
Hugo Pereira, Katkam N Gangadhar, Peter S C Schulze, Tamára Santos, Carolina Bruno de Sousa, Lisa M Schueler, Luísa Custódio, F Xavier Malcata, Luísa Gouveia, João C S Varela, Luísa Barreira
Bioprospecting for novel microalgal strains is key to improving the feasibility of microalgae-derived biodiesel production. Tetraselmis sp. CTP4 (Chlorophyta, Chlorodendrophyceae) was isolated using fluorescence activated cell sorting (FACS) in order to screen novel lipid-rich microalgae. CTP4 is a robust, euryhaline strain able to grow in seawater growth medium as well as in non-sterile urban wastewater. Because of its large cell size (9-22 μm), CTP4 settles down after a six-hour sedimentation step. This leads to a medium removal efficiency of 80%, allowing a significant decrease of biomass dewatering costs...
October 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27757268/in-vitro-antileishmanial-and-antimalarial-activity-of-selected-plants-of-nepal
#19
Bishnu Joshi, Sarah Hendrickx, Lila Bahadur Magar, Niranjan Parajuli, Pierre Dorny, Louis Maes
BACKGROUND: Nepal is very rich in biodiversity, and no extensive effort has yet been carried out to screen plants that are used by traditional healers against parasitic diseases. The aim of this study was to evaluate the in vitro antileishmanial and antimalarial activity of crude methanolic or ethanolic extracts of 29 plant species that are currently used by local people of Nepal for treating different ailments. METHODS: Crude extracts of leaves, twigs, aerial parts, and/or roots of the selected plants were evaluated for in vitro inhibitory activity against intracellular amastigotes of Leishmania infantum and against erythrocytic stages of Plasmodium falciparum...
September 2016: Journal of Intercultural Ethnopharmacology
https://www.readbyqxmd.com/read/27702563/bioprospecting-for-secondary-metabolites-in-the-entomopathogenic-bacterium-photorhabdus-luminescens-subsp-sonorensis
#20
Rousel A Orozco, István Molnár, Helge Bode, S Patricia Stock
Crude extracts of in vitro and in vivo cultures of two strains of Photorhabdus l. sonorensis (Enterobacteriaceae) were analyzed by TLC, HPLC-UV and LC-MS. Nine unique compounds with mass/charge ratios (m/z) ranging from 331.3 to 713.5 were found in MS analyses. Bioactivity of extracts was assessed on a selection of plant pathogens/pests and non-target species. Caborca strain extracts showed the highest activity against Helicoverpa zea (Lepidoptera: Noctuidae) neonates at all concentrations tested. Mortality ranged from 11% (at 10μg/ml) to 37% (at 40μg/ml)...
November 2016: Journal of Invertebrate Pathology
keyword
keyword
5077
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"