keyword
MENU ▼
Read by QxMD icon Read
search

Bioprospecting

keyword
https://www.readbyqxmd.com/read/29228814/cold-adapted-enzymes-produced-by-fungi-from-terrestrial-and-marine-antarctic-environments
#1
Alysson Wagner Fernandes Duarte, Juliana Aparecida Dos Santos, Marina Vitti Vianna, Juliana Maíra Freitas Vieira, Vitor Hugo Mallagutti, Fabio José Inforsato, Lia Costa Pinto Wentzel, Luciana Daniela Lario, Andre Rodrigues, Fernando Carlos Pagnocca, Adalberto Pessoa Junior, Lara Durães Sette
Antarctica is the coldest, windiest, and driest continent on Earth. In this sense, microorganisms that inhabit Antarctica environments have to be adapted to harsh conditions. Fungal strains affiliated with Ascomycota and Basidiomycota phyla have been recovered from terrestrial and marine Antarctic samples. They have been used for the bioprospecting of molecules, such as enzymes. Many reports have shown that these microorganisms produce cold-adapted enzymes at low or mild temperatures, including hydrolases (e...
December 11, 2017: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/29209209/nonribosomal-peptides-from-marine-microbes-and-their-antimicrobial-and-anticancer-potential
#2
REVIEW
Shivankar Agrawal, Debabrata Acharya, Alok Adholeya, Colin J Barrow, Sunil K Deshmukh
Marine environments are largely unexplored and can be a source of new molecules for the treatment of many diseases such as malaria, cancer, tuberculosis, HIV etc. The Marine environment is one of the untapped bioresource of getting pharmacologically active nonribosomal peptides (NRPs). Bioprospecting of marine microbes have achieved many remarkable milestones in pharmaceutics. Till date, more than 50% of drugs which are in clinical use belong to the nonribosomal peptide or mixed polyketide-nonribosomal peptide families of natural products isolated from marine bacteria, cyanobacteria and fungi...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29182315/determinants-and-prediction-of-esterase-substrate-promiscuity-patterns
#3
Monica Martínez-Martínez, Cristina Coscolín, Gerard Santiago, Jennifer Chow, Peter J Stogios, Rafael Bargiela, Christoph Gertler, José Navarro-Fernández, Alexander Bollinger, Stephan Thies, Celia Méndez-García, Ana Popovic, Greg Brown, Tatyana N Chernikova, Antonio García-Moyano, Gro Ek Bjergah, Pablo Pérez-García, Tran Hai, Mercedes V Del Pozo, Runar Stokke, Ida H Steen, Hong Cui, Xiaohui Xu, Boguslaw P Nocek, María Alcaide, Marco Distaso, Victoria Mesa, Ana I Pelaez, Jesús Sánchez, Patrick C F Buchholz, Juergen Pleiss, Antonio Fernández-Guerra, Frank O Glöckner, Olga V Golyshina, Michail M Yakimov, Alexei Savchenko, Karl-Erich Jaeger, Alexander F Yakunin, Wolfgang R Streit, Peter N Golyshin, Victor Guallar, Manuel Ferrer
Esterases receive special attention because their wide distribution in biological systems and environments and their importance for physiology and chemical synthesis. The prediction of esterases substrate promiscuity level from sequence data and the molecular reasons why certain such enzymes are more promiscuous than others, remain to be elucidated. This limits the surveillance of the sequence space for esterases potentially leading to new versatile biocatalysts and new insights into their role in cellular function...
November 28, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/29181803/functional-characterization-of-probiotic-potential-of-novel-pigmented-bacterial-strains-for-aquaculture-applications
#4
Sekar Jinendiran, Seenivasan Boopathi, Natesan Sivakumar, Gopal Selvakumar
The bioprospecting proficient of novel pigmented probiotic strains with respect to aquaculture industry was unexplored hitherto. In this study, we investigated the probiotic potential of novel pigmented bacterial strains isolated from the indigenous soil sediments in their vicinal habitats, which were screened for their antimicrobial activity against aquatic pathogens using agar well diffusion assay. The strains namely Exiguobacterium acetylicum (S01), Aeromonas veronii (V03), and Chryseobacterium joostei (V04) were phenotypically identified and confirmed by 16S rRNA gene sequence analysis...
November 27, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29157056/the-intron-landscape-of-the-mtdna-cytb-gene-among-the-ascomycota-introns-and-intron-encoded-open-reading-frames
#5
Tuhin K Guha, Alvan Wai, Sahra-Taylor Mullineux, Georg Hausner
Fungal mitochondrial genes are frequently noted for the presence of introns. These introns are self-splicing and can be assigned to either group I or II introns and they can encode open reading frames (ORFs). This study examines the introns present within the cytochrome b (cytb) gene of ascomycetes fungi. Cytochrome b gene sequences were sampled from GenBank and supplemented with our own data for species of Leptographium and Ophiostoma. Group I introns were encountered most frequently, many encoding either LAGLIDADG or GIY-YIG homing endonucleases (HEs)...
November 20, 2017: Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
https://www.readbyqxmd.com/read/29152156/revisiting-the-linkage-between-ethnomedical-use-and-development-of-new-medicines-a-novel-plant-collection-strategy-towards-the-discovery-of-anticancer-agents
#6
Joshua M Henkin, Kongmany Sydara, Mouachanh Xayvue, Onevilay Souliya, A Douglas Kinghorn, Joanna E Burdette, Wei-Lun Chen, Bethany G Elkington, Djaja D Soejarto
The Vietnam-Laos International Cooperative Biodiversity Group (ICBG) based at the University of Illinois at Chicago (UIC) catalyzed a country-wide network of medicinal plant preserves (MPP) and medicinal biodiversity preserves (MBP) now established in ten provinces of the Lao People's Democratic Republic (Lao PDR), which are relied upon as protected sources of ethnomedicines for local villagers and traditional healers. In collaboration with the Lao PDR's Institute of Traditional Medicine (ITM), our ongoing P01 Program Project (Ohio State University) examined the anticancer bioprospecting potential for two of the most exhaustively inventoried of these sites: the Bolikhamxay MPP and the Xiengkhouang MBP...
October 2017: Journal of Medicinal Plant Research
https://www.readbyqxmd.com/read/29124514/anaerobic-microplate-assay-for-direct-microbial-conversion-of-switchgrass-and-avicel-using-clostridium-thermocellum
#7
Gbekeloluwa B Oguntimein, Miguel Rodriguez, Alexandru Dumitrache, Todd Shollenberger, Stephen R Decker, Brian H Davison, Steven D Brown
OBJECTIVE: To develop and prototype a high-throughput microplate assay to assess anaerobic microorganisms and lignocellulosic biomasses in a rapid, cost-effective screen for consolidated bioprocessing potential. RESULTS: Clostridium thermocellum parent Δhpt strain deconstructed Avicel to cellobiose, glucose, and generated lactic acid, formic acid, acetic acid and ethanol as fermentation products in titers and ratios similar to larger scale fermentations confirming the suitability of a plate-based method for C...
November 9, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/29093750/bioprospecting-of-novel-thermostable-%C3%AE-glucosidase-from-bacillus-subtilis-ra10-and-its-application-in-biomass-hydrolysis
#8
Rameshwar Tiwari, Puneet Kumar Singh, Surender Singh, Pawan K S Nain, Lata Nain, Pratyoosh Shukla
Background: Saccharification is the most crucial and cost-intensive process in second generation biofuel production. The deficiency of β-glucosidase in commercial enzyme leads to incomplete biomass hydrolysis. The decomposition of biomass at high temperature environments leads us to isolate thermotolerant microbes with β-glucosidase production potential. Results: A total of 11 isolates were obtained from compost and cow dung samples that were able to grow at 50 °C...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29077533/the-ethnopharmacologic-contribution-to-bioprospecting-natural-products
#9
Eric J Buenz, Rob Verpoorte, Brent A Bauer
Descriptions of the use of natural products in traditional medicine have served as starting points for new therapeutics. The details of the traditional use of these organisms can provide important information for future drug discovery and development efforts. Recent technologic advances provide the framework to leverage ethnopharmacologic data in the drug discovery process. Information on the traditional harvest, preparation, storage, and administration of the organisms, and the natural products they contain, provides valuable details regarding characteristics of the active compounds...
October 27, 2017: Annual Review of Pharmacology and Toxicology
https://www.readbyqxmd.com/read/29062114/bioprospecting-on-invasive-plant-species-to-prevent-seed-dispersal
#10
Lorenzo Guzzetti, Andrea Galimberti, Ilaria Bruni, Chiara Magoni, Maura Ferri, Annalisa Tassoni, Enrico Sangiovanni, Mario Dell' Agli, Massimo Labra
The most anthropized regions of the world are characterized by an impressive abundance of invasive plants, which alter local biodiversity and ecosystem services. An alternative strategy to manage these species could be based on the exploitation of their fruits in a framework of bioprospecting to obtain high-added value compounds or phytocomplexes that are useful for humans. Here we tested this hypothesis on three invasive plants (Lonicera japonica Thunb., Phytolacca americana L., and Prunus serotina Ehrh.) in the Po plain (northern Italy) which bear fruits that are highly consumed by frugivorous birds and therefore dispersed over large distances...
October 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29026943/quorum-sensing-inhibitors-can-endophytes-be-prospective-sources
#11
Abhirup Mookherjee, Shivangi Singh, Mrinal K Maiti
Endophytes are microbes which reside inside the plant tissues asymptomatically or causing pathogenicity to the host plant for a brief period. Owing to their presence in a specialized niche, endophytes are capable of synthesizing diverse types of bioactive molecules. Continuous development of resistance mechanism by pathogens to the currently available health treatments and pharmaceuticals has led researchers to explore new therapeutic agents. Quorum sensing has a role in the development of microbial pathogenic traits including biofilm formation...
October 12, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/29025413/carbohydrate-active-enzymes-in-trichoderma-harzianum-a-bioinformatic-analysis-bioprospecting-for-key-enzymes-for-the-biofuels-industry
#12
Jaire Alves Ferreira Filho, Maria Augusta Crivelente Horta, Lilian Luzia Beloti, Clelton Aparecido Dos Santos, Anete Pereira de Souza
BACKGROUND: Trichoderma harzianum is used in biotechnology applications due to its ability to produce powerful enzymes for the conversion of lignocellulosic substrates into soluble sugars. Active enzymes involved in carbohydrate metabolism are defined as carbohydrate-active enzymes (CAZymes), and the most abundant family in the CAZy database is the glycoside hydrolases. The enzymes of this family play a fundamental role in the decomposition of plant biomass. RESULTS: In this study, the CAZymes of T...
October 12, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28993256/biochemical-characterization-and-low-resolution-saxs-structure-of-an-exo-polygalacturonase-from-bacillus-licheniformis
#13
Danilo Elton Evangelista, Evandro A de Araújo, Mario Oliveira Neto, Marco Antonio Seiki Kadowaki, Igor Polikarpov
Among the structural polymers present in the plant cell wall, pectin is the main component of the middle lamella. This heterogeneous polysaccharide has an α-1,4 galacturonic acid backbone, which can be broken by the enzymatic action of pectinases, such as exo-polygalacturonases, that sequentially cleave pectin from the non-reducing ends, releasing mono or di-galacturonic acid residues. Constant demand for pectinases that better suit industrial requirements has motivated identification and characterization of novel enzymes from diverse sources...
October 6, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28989862/bioprospecting-north-atlantic-microalgae-with-fast-growth-and-high-polyunsaturated-fatty-acid-pufa-content-for-microalgae-based-technologies
#14
Pia Steinrücken, Svein Rune Erga, Svein Are Mjøs, Hans Kleivdal, Siv Kristin Prestegard
Microalgae are considered to be an important and sustainable alternative to fish oil as a source for the polyunsaturated fatty acids (PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Due to their health benefits, there is an increasing interest in the commercial application of these fatty acids (FA) to health and dietary products, and to aquaculture feeds. However, FA from microalgae are still expensive to produce compared to fish or plant oils. With only a few microalgal strains being cultivated on a large scale for commercial PUFA production, prospecting for new, robust and fast-growing strains with increased PUFA content is essential in order to reduce production costs...
September 2017: Algal Research
https://www.readbyqxmd.com/read/28955634/bioprospecting-endophytic-diazotrophic-lysinibacillus-sphaericus-as-biocontrol-agents-of-rice-sheath-blight-disease
#15
S Shabanamol, J Sreekumar, M S Jisha
The present study tried to explore the possible in vitro biocontrol mechanisms of Lysinibacillus sphaericus, a diazotrophic endophyte from rice against the rice sheath blight pathogen Rhizoctonia solani. The in vivo biocontrol potential of the isolate and the induction of systemic resistance under greenhouse conditions have also been experimented employing different treatments with positive control carbendazim, the chemical fungicide. The endophytic isolate showed 100% growth inhibition of the fungal pathogen via volatile organic compound production and was positive for the production of siderophores, biosurfactants, HCN, and ammonia...
October 2017: 3 Biotech
https://www.readbyqxmd.com/read/28953997/antimycobacterial-and-antimalarial-activities-of-endophytic-fungi-associated-with-the-ancient-and-narrowly-endemic-neotropical-plant-vellozia-gigantea-from-brazil
#16
Mariana C Ferreira, Charles L Cantrell, David E Wedge, Vívian N Gonçalves, Melissa R Jacob, Shabana Khan, Carlos A Rosa, Luiz H Rosa
BACKGROUND: Endophytic fungi, present mainly in the Ascomycota and Basidiomycota phyla, are associated with different plants and represent important producers of bioactive natural products. Brazil has a rich biodiversity of plant species, including those reported as being endemic. Among the endemic Brazilian plant species, Vellozia gigantea (Velloziaceae) is threatened by extinction and is a promising target to recover endophytic fungi. OBJECTIVE: The present study focused on bioprospecting of bioactive compounds of the endophytic fungi associated with V...
October 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28952531/molecular-diagnostic-for-prospecting-polyhydroxyalkanoate-producing-bacteria
#17
Eduarda Morgana da Silva Montenegro, Gabriela Scholante Delabary, Marcus Adonai Castro da Silva, Fernando Dini Andreote, André Oliveira de Souza Lima
The use of molecular diagnostic techniques for bioprospecting and microbial diversity study purposes has gained more attention thanks to their functionality, low cost and quick results. In this context, ten degenerate primers were designed for the amplification of polyhydroxyalkanoate synthase (phaC) gene, which is involved in the production of polyhydroxyalkanoate (PHA)-a biodegradable, renewable biopolymer. Primers were designed based on multiple alignments of phaC gene sequences from 218 species that have their genomes already analyzed and deposited at Biocyc databank...
May 25, 2017: Bioengineering
https://www.readbyqxmd.com/read/28930185/bioprospecting-for-exopolysaccharides-from-deep-sea-hydrothermal-vent-bacteria-relationship-between-bacterial-diversity-and-chemical-diversity
#18
Christine Delbarre-Ladrat, Marcia Leyva Salas, Corinne Sinquin, Agata Zykwinska, Sylvia Colliec-Jouault
Many bacteria biosynthesize structurally diverse exopolysaccharides (EPS) and excrete them into their surrounding environment. The EPS functional features have found many applications in industries such as cosmetics and pharmaceutics. In particular, some EPS produced by marine bacteria are composed of uronic acids, neutral sugars, and N-acetylhexosamines, and may also bear some functional sulfate groups. This suggests that they can share common structural features with glycosaminoglycans (GAG) like the two EPS (HE800 and GY785) originating from the deep sea...
September 20, 2017: Microorganisms
https://www.readbyqxmd.com/read/28918535/bioprospecting-of-novel-and-bioactive-compounds-from-marine-actinomycetes-isolated-from-south-china-sea-sediments
#19
Na Yang, Fuhang Song
Marine actinomycetes are less investigated compared to terrestrial strains as potential sources of natural products. To date, few investigations have been performed on culturable actinomycetes associated with South China Sea sediments. In the present study, twenty-eight actinomycetes were recovered from South China Sea sediments after dereplication by traditional culture-dependent method. The 16S rRNA gene sequences analyses revealed that these strains related to five families and seven genera. Twelve representative strains possessed at least one of the biosynthetic genes coding for polyketide synthase I, II, and nonribosomal peptide synthetase...
September 16, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28892512/grape-microbiome-as-a-reliable-and-persistent-signature-of-field-origin-and-environmental-conditions-in-cannonau-wine-production
#20
Valerio Mezzasalma, Anna Sandionigi, Ilaria Bruni, Antonia Bruno, Gianni Lovicu, Maurizio Casiraghi, Massimo Labra
Grape berries harbor a wide range of microbes originating from the vineyard environment, many of which are recognized for their role in the must fermentation process shaping wine quality. To better clarify the contribution of the microbiome of grape fruits during wine fermentation, we used high-throughput sequencing to identify bacterial and fungi communities associated with berries and musts of Cannonau. This is the most important cultivar-wine of Sardinia (Italy) where most vineyards are cultivated without phytochemical treatments...
2017: PloS One
keyword
keyword
5077
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"