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https://www.readbyqxmd.com/read/28814644/subgenome-dominance-in-an-interspecific-hybrid-synthetic-allopolyploid-and-a-140-year-old-naturally-established-neo-allopolyploid-monkeyflower
#1
Patrick P Edger, Ronald D Smith, Michael R McKain, Arielle M Cooley, Mario Vallejo-Marin, Yao-Wu Yuan, Adam J Bewick, Lexiang Ji, Adrian E Platts, Megan J Bowman, Kevin Childs, Jacob D Washburn, Robert Schmitz, Gregory D Smith, J Chris Pires, Joshua R Puzey
Recent studies have shown that one of the parental subgenomes in ancient polyploids is generally more dominant - having retained more genes and being more highly expressed - a phenomenon termed subgenome dominance. The genomic features that determine how quickly and which subgenome dominates within a newly formed polyploid remain poorly understood. To investigate the rate of emergence of subgenome dominance, we examined gene expression, gene methylation, and transposable element (TE) methylation in a natural, less than 140 year old allopolyploid (Mimulus peregrinus), a resynthesized inter-species triploid hybrid (M...
August 16, 2017: Plant Cell
https://www.readbyqxmd.com/read/28811632/atomic-scale-study-of-the-amorphous-to-crystalline-phase-transition-mechanism-in-gete-thin-films
#2
R Mantovan, R Fallica, A Mokhles Gerami, T E Mølholt, C Wiemer, M Longo, H P Gunnlaugsson, K Johnston, H Masenda, D Naidoo, M Ncube, K Bharuth-Ram, M Fanciulli, H P Gislason, G Langouche, S Ólafsson, G Weyer
The underlying mechanism driving the structural amorphous-to-crystalline transition in Group VI chalcogenides is still a matter of debate even in the simplest GeTe system. We exploit the extreme sensitivity of (57)Fe emission Mössbauer spectroscopy, following dilute implantation of (57)Mn (T½ = 1.5 min) at ISOLDE/CERN, to study the electronic charge distribution in the immediate vicinity of the (57)Fe probe substituting Ge (FeGe), and to interrogate the local environment of FeGe over the amorphous-crystalline phase transition in GeTe thin films...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28809132/impact-of-primary-tracheoesophageal-puncture-on-outcomes-after-total-laryngectomy
#3
Aru Panwar, Oleg Militsakh, Robert Lindau, Andrew Coughlin, Harlan Sayles, Katherine R Rieke, William Lydiatt, Daniel Lydiatt, Russell Smith
Objectives To identify differences in postoperative wound complications associated with a primary tracheoesophageal puncture (TEP) at the time of laryngectomy versus no TEP. Study Design Retrospective review of large national data set. Setting Academic and nonacademic health care facilities in United States, contributing de-identified, risk-adjusted clinical data to the American College of Surgeons National Surgical Quality Improvement Program. Subjects and Methods The National Surgical Quality Improvement Program data set for years 2006 to 2012 identified 430 patients who underwent total laryngectomy with or without a primary TEP...
August 1, 2017: Otolaryngology—Head and Neck Surgery
https://www.readbyqxmd.com/read/28807203/utilizing-stricture-indices-to-predict-dilation-of-strictures-after-esophageal-atresia-repair
#4
Rachel M Landisch, Sheila Foster, David Gregg, Thomas Chelius, Laura D Cassidy, Diana Lerner, Dave R Lal
BACKGROUND: Anastomotic stricture is the most common postoperative complication in infants undergoing repair of esophageal atresia with or without tracheoesophageal fistula (EA/TEF). Stricture indices (SIs) are used to predict infants at risk for stricture requiring dilation. We sought to determine the most accurate SI and optimal timing for predicting anastomotic dilation. MATERIALS AND METHODS: A retrospective study of infants undergoing repair of EA/TEF between 2008 and 2013 was performed...
August 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/28795562/axially-bound-ruthenium-phthalocyanine-monolayers-on-indium-tin-oxide-structure-energetics-and-charge-transfer-properties
#5
Ramanan Ehamparam, Luis E Oquendo, Michael W Liao, Ambjorn Karl Brynnel, Kai-Lin Ou, Neal R Armstrong, Dominic V McGrath, S Scott Saavedra
The efficiency of charge collection at the organic/transparent conducting oxide (TCO) interface in organic photovoltaic (OPV) devices affects overall device efficiency. Modifying the TCO with an electrochemically active molecule may enhance OPV efficiency by providing a charge-transfer pathway between the electrode and the organic active layer, and may also mitigate surface recombination. The synthesis and characterization of phosphonic acid-ruthenium phthalocyanine (RuPcPA) monolayer films on indium tin oxide (ITO), designed to facilitate charge harvesting at ITO electrodes, is presented in this work...
August 10, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28765607/suppression-of-transposable-elements-in-leukemic-stem-cells
#6
Anthony R Colombo, Asif Zubair, Devi Thiagarajan, Sergey Nuzhdin, Timothy J Triche, Giridharan Ramsingh
Genomic transposable elements (TEs) comprise nearly half of the human genome. The expression of TEs is considered potentially hazardous, as it can lead to insertional mutagenesis and genomic instability. However, recent studies have revealed that TEs are involved in immune-mediated cell clearance. Hypomethylating agents can increase the expression of TEs in cancer cells, inducing 'viral mimicry', causing interferon signalling and cancer cell killing. To investigate the role of TEs in the pathogenesis of acute myeloid leukaemia (AML), we studied TE expression in several cell fractions of AML while tracking its development (pre-leukemic haematopoietic stem cells, leukemic stem cells [LSCs], and leukemic blasts)...
August 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28759632/significant-liver-fibrosis-assessed-using-liver-transient-elastography-is-independently-associated-with-low-bone-mineral-density-in-patients-with-non-alcoholic-fatty-liver-disease
#7
Gyuri Kim, Kwang Joon Kim, Yumie Rhee, Sung-Kil Lim
BACKGROUND: Metabolic bone disorders frequently occur in patients with chronic liver disease; however, the association between liver fibrosis and bone mineral density in patients with non-alcoholic fatty liver disease (NAFLD) is unclear. METHODS: This is a cross-sectional analysis of 231 asymptomatic subjects (160 women, 61.6 years old) from a university hospital setting, between February 2012 and December 2014. Bone mineral density (BMD) was measured at the lumbar spine, femur neck, and total hip using dual-energy X-ray absorptiometry (DXA)...
2017: PloS One
https://www.readbyqxmd.com/read/28751108/processing-of-mutant-n-acetyl-%C3%AE-glucosaminidase-in-mucopolysaccharidosis-type-iiib-fibroblasts-cultured-at-low-temperature
#8
O L M Meijer, H Te Brinke, R Ofman, L IJlst, F A Wijburg, N van Vlies
BACKGROUND: The autosomal recessive, neurodegenerative disorder mucopolysaccharidosis type IIIB (MPSIIIB) is caused by a deficiency of the lysosomal enzyme N-acetyl-α-glucosaminidase (NAGLU), resulting in accumulation of heparan sulfate. The disease spectrum comprises a severe, rapidly progressing (RP) phenotype and a more attenuated, slowly progressing (SP) phenotype. Previous studies showed significantly higher NAGLU activity in skin fibroblasts of SP patients when cultured at 30°C which may be relevant for development of novel therapeutic strategies...
July 12, 2017: Molecular Genetics and Metabolism
https://www.readbyqxmd.com/read/28747133/retroperitoneal-laparoscopic-nephrotomy-along-the-brodel-line-and-tumor-enucleation-te-for-complete-intra-parenchymal-renal-tumors-a-single-institution-experience
#9
Baojun Wang, Huijie Gong, Xin Ma, Hong Zhao Li, Yongji Yan, Wei Cai, Lixin Shi, Xintao Li, Xu Zhang
PURPOSE: To evaluate the feasibility and outcomes of retroperitoneal laparoscopic nephrotomy along the Brodel line and tumor enucleation (TE) for complete intra-parenchymal renal tumors. PATIENTS AND METHODS: We retrospectively analysed the medical charts of patients with complete endophytic tumors and who underwent retroperitoneal laparoscopic nephrotomy along the Brodel line and TE between 2012 and 2015. Peri-operative data, surgical technique, pathological variables, complications, functional and oncological outcomes were reviewed...
July 26, 2017: Journal of Endourology
https://www.readbyqxmd.com/read/28740794/tissue-expanders-and-proton-beam-radiotherapy-what-you-need-to-know
#10
Ashley L Howarth, Joshua R Niska, Kenneth Brooks, Aman Anand, Martin Bues, Carlos E Vargas, Raman C Mahabir
Proton beam radiotherapy (PBR) has gained acceptance for the treatment of breast cancer because of unique beam characteristics that allow superior dose distributions with optimal dose to the target and limited collateral damage to adjacent normal tissue, especially to the heart and lungs. To determine the compatibility of breast tissue expanders (TEs) with PBR, we evaluated the structural and dosimetric properties of 2 ex vivo models: 1 model with internal struts and another model without an internal structure...
June 2017: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/28737809/hybrid-density-functional-theory-modeling-of-ca-zn-and-al-ion-batteries-using-the-chevrel-phase-mo6s8-cathode
#11
Taylor R Juran, Manuel Smeu
Hybrid density functional theory (DFT) is used to study the Chevrel phase Mo6X8 (X = S, Se, Te) as a promising cathode material intercalated with various metal ions (M = Li, Na, Be, Mg, Ca, Sr, Ba, Zn, Al). Electronic properties and voltages are calculated for each case. Ca ions are predicted to produce a voltage output ranging from 1.8-2.1 V, comparable to the voltage calculated for Li ions while providing two electrons per transferred ion. The highest voltage is determined to result when the chalcogen X in Mo6X8 is S, over Se or Te...
August 9, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28726382/interparticle-reactions-an-emerging-direction-in-nanomaterials-chemistry
#12
K R Krishnadas, Ananya Baksi, Atanu Ghosh, Ganapati Natarajan, Anirban Som, Thalappil Pradeep
Nanoparticles exhibit a rich variety in terms of structure, composition, and properties. However, reactions between them remain largely unexplored. In this Account, we discuss an emerging aspect of nanomaterials chemistry, namely, interparticle reactions in solution phase, similar to reactions between molecules, involving atomically precise noble metal clusters. A brief historical account of the developments, starting from the bare, gas phase clusters, which led to the synthesis of atomically precise monolayer protected clusters in solution, is presented first...
July 20, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28718991/learning-curve-for-the-thoracoscopic-repair-of-esophageal-atresia-with-tracheoesophageal-fistula
#13
Hiroomi Okuyama, Yuko Tazuke, Takehisa Ueno, Hiroaki Yamanaka, Yuichi Takama, Ryuta Saka, Noriaki Usui, Hideki Soh, Takeo Yonekura
AIM: Thoracoscopic repair (TR) of esophageal atresia with tracheoesophageal fistula (EA/TEF) remains a considerable challenge, even for the most experienced pediatric surgeons. The aim of this study is to report the outcomes of our experience with TR of EA/TEF and to determine the learning curve for this procedure. METHODS: Eleven consecutive cases that had undergone TR of EA/TEF at our institutes were included in this study. The medical charts were reviewed retrospectively...
July 18, 2017: Asian Journal of Endoscopic Surgery
https://www.readbyqxmd.com/read/28714539/safety-and-efficacy-of-functional-laryngectomy-for-end-stage-dysphagia
#14
Michael C Topf, Linda C Magaña, Kelly Salmon, James Hamilton, William M Keane, Adam Luginbuhl, Joseph M Curry, David M Cognetti, Maurits Boon, Joseph R Spiegel
OBJECTIVES/HYPOTHESIS: To evaluate functional outcomes and complication rate after total laryngectomy (TL) for dysfunctional larynx with end-stage dysphagia. STUDY DESIGN: Retrospective chart review. METHODS: Chart review was performed on all patients who underwent TL from January 2008 to July 2016 at a single tertiary academic medical center. Patients who underwent TL for dysfunctional larynx without preoperative evidence of malignancy were included...
July 17, 2017: Laryngoscope
https://www.readbyqxmd.com/read/28709660/effect-of-gastrointestinal-malformations-on-the-outcomes-of-patients-with-congenital-heart-disease
#15
Carlos M Mery, Luis E De León, J Rubén Rodriguez, R Michael Nieto, Wei Zhang, Iki Adachi, Jeffrey S Heinle, Lauren C Kane, E Dean McKenzie, Charles D Fraser
BACKGROUND: The goal of this study was to assess the effect of associated gastrointestinal malformations (GI) on the outcomes of patients undergoing congenital heart operations. METHODS: Neonates and infants with thoracic (esophageal atresia, tracheoesophageal fistula) and abdominal (duodenal stenosis/atresia, imperforate anus, Hirschsprung disease) GI malformations undergoing congenital heart operations between 1995 and 2015 were included. Two control groups were created, one for each group...
July 11, 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28700459/the-microbiome-in-posttraumatic-stress-disorder-and-trauma-exposed-controls-an-exploratory-study
#16
Sian M J Hemmings, Stefanie Malan-Muller, Leigh L van den Heuvel, Brittany A Demmitt, Maggie A Stanislawski, David G Smith, Adam D Bohr, Christopher E Stamper, Embriette R Hyde, James T Morton, Clarisse A Marotz, Philip H Siebler, Maarten Braspenning, Wim Van Criekinge, Andrew J Hoisington, Lisa A Brenner, Teodor T Postolache, Matthew B McQueen, Kenneth S Krauter, Rob Knight, Soraya Seedat, Christopher A Lowry
OBJECTIVE: Inadequate immunoregulation and elevated inflammation may be risk factors for posttraumatic stress disorder (PTSD), and microbial inputs are important determinants of immunoregulation; however, the association between the gut microbiota and PTSD is unknown. This study investigated the gut microbiome in a South African sample of PTSD-affected individuals and trauma-exposed (TE) controls, to identify potential differences in microbial diversity or microbial community structure...
July 11, 2017: Psychosomatic Medicine
https://www.readbyqxmd.com/read/28698025/bifurcation-of-the-respiratory-response-to-lung-inflation-in-anesthetized-dogs
#17
Jaroslaw R Romaniuk, Thomas E Dick, Eugene N Bruce, Anthony F DiMarco, Krzysztof E Kowalski
Numerous studies have demonstrated the effect of lung volume on prolongation of duration of expiration (TE) with limited understanding of the TE shortening and termination of expiration as observed in newborn. In 14 dogs, the effects of varied onset of lung inflation during expiration on the TE were evaluated. When lung inflation was applied in the first part of expiration (20-60% of TE) TE was lengthened. However, in the second portion (60-80% of TE) of expiration, lung inflation either terminated or prolonged TE; whereas in the last portion of expiration (80-90% of TE), lung inflation tended to terminate expiration prematurely...
July 8, 2017: Respiratory Physiology & Neurobiology
https://www.readbyqxmd.com/read/28690313/the-hdac-inhibitor-panobinostat-lbh589-exerts-in-vivo-anti-leukaemic-activity-against-mll-rearranged-acute-lymphoblastic-leukaemia-and-involves-the-rnf20-rnf40-wac-h2b-ubiquitination-axis
#18
P G Castro, E H J van Roon, S S M Pinhanços, L Trentin, P Schneider, M Kerstjens, G Te Kronnie, O Heidenreich, R Pieters, R W Stam
MLL-rearranged acute lymphoblastic leukaemia (ALL) represents an aggressive malignancy in infants (<1 year of age), associated with poor outcome. Current treatment intensification is not further possible, and novel therapy strategies are needed. Notably, MLL-rearranged ALL is characterised by a strongly deregulated epigenome and shows sensitivity to epigenetic perturbators. Here, we demonstrate the in vivo efficacy of the histone deacetylase inhibitor Panobinostat (LHB589) using xenograft mouse models of MLL-rearranged ALL...
July 10, 2017: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
https://www.readbyqxmd.com/read/28683354/known-knowns-known-unknowns-and-unknown-unknowns-in-prokaryotic-transposition
#19
REVIEW
Patricia Siguier, Edith Gourbeyre, Michael Chandler
Although the phenomenon of transposition has been known for over 60 years, its overarching importance in modifying and streamlining genomes took some time to recognize. In spite of a robust understanding of transposition of some TE, there remain a number of important TE groups with potential high genome impact and unknown transposition mechanisms and yet others, only recently identified by bioinformatics, yet to be formally confirmed as mobile. Here, we point to some areas of limited understanding concerning well established important TE groups with DDE Tpases, to address central gaps in our knowledge of characterised Tn with other types of Tpases and finally, to highlight new potentially mobile DNA species...
July 3, 2017: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/28683188/photochemical-water-splitting-by-bismuth-chalcogenide-topological-insulators
#20
Catherine R Rajamathi, Uttam Gupta, Koushik Pal, Nitesh Kumar, Hao Yang, Yan Sun, Chandra Shekhar, Binghai Yan, Stuart Parkin, Umesh V Waghmare, Claudia Felser, C N R Rao
As one of the major areas of interest in catalysis revolves around 2D materials based on molybdenum sulfide, we have examined the catalytic properties of bismuth selenides and tellurides which are among the first chalcogenides to be proven as topological insulators (TIs). We find significant photochemical H₂ evolution activity with these TIs as catalysts. H₂ evolution increases drastically in nanosheets of Bi₂Te₃ compared to single crystals. First-principles calculations show that due to the topology, surface states participate and promote the hydrogen evolution...
July 6, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
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