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Lin Cheng, Matthew D Blackledge, David J Collins, Matthew R Orton, Neil P Jerome, Thorsten Feiweier, Mihaela Rata, Veronica Morgan, Nina Tunariu, Martin O Leach, Dow-Mu Koh
PURPOSE: To introduce T2-adjusted computed DWI (T2-cDWI), a method that provides synthetic images at arbitrary b-values and echo times (TEs) that improve tissue contrast by removing or increasing T2 contrast in diffusion-weighted images. MATERIALS AND METHODS: In addition to the standard DWI acquisition protocol T2-weighted echo-planar images at multiple (≥2) echo times were acquired. This allows voxelwise estimation of apparent diffusion coefficient (ADC) and T2 values, permitting synthetic images to be generated at any chosen b-value and echo time...
October 1, 2016: Computers in Biology and Medicine
Te-Chih Wong, Hsiu-Yueh Su, Yu-Tong Chen, Pei-Yu Wu, Hsi-Hsien Chen, Tso-Hsiao Chen, Yung-Ho Hsu, Shwu-Huey Yang
Recent studies have indicated that the ratio of C-reactive protein to albumin (CRP-Alb ratio) is associated with clinical outcomes in patients with disease. We examined the predictive value of this ratio in patients undergoing hemodialysis (HD). In this cross-sectional study, 91 eligible adult HD patients were analyzed, and the correlation between the CRP-Alb ratio and skeletal muscle mass normalized for body weight (SMM/wt; estimated using a bioelectrical impedance analyzer) was investigated. The mean age of the study participants was 54...
2016: PloS One
Jingzhong Xie, Naxin Huo, Shenghui Zhou, Yi Wang, Guanghao Guo, Karin R Deal, Shuhong Ouyang, Yong Liang, Zhenzhong Wang, Lichan Xiao, Tingting Zhu, Tiezhu Hu, Vijay Tiwari, Jianwei Zhang, Hongxia Li, Zhongfu Ni, Yingyin Yao, Huiru Peng, Shengli Zhang, Olin D Anderson, Patrick E McGuire, Jan Dvorak, Ming-Cheng Luo, Zhiyong Liu, Yong Q Gu, Qixin Sun
Bread wheat (Triticum aestivum, AABBDD) is an allohexaploid species derived from two rounds of interspecific hybridizations. A high-quality genome sequence assembly of diploid Aegilops tauschii, the donor of the wheat D genome, will provide a useful platform to study polyploid wheat evolution. A combined approach of BAC pooling and next-generation sequencing technology was employed to sequence the minimum tiling path (MTP) of 3176 BAC clones from the short arm of Ae. tauschii chromosome 3 (At3DS). The final assembly of 135 super-scaffolds with an N50 of 4...
October 5, 2016: Journal of Genetics and Genomics, Yi Chuan Xue Bao
John R Broughton, Herenia P Lawrence, Lisa Jamieson
: Early childhood caries is a global health issue for Indigenous populations. The study, "Reducing disease burden and health inequalities arising from chronic dental disease among Indigenous children: an early childhood caries intervention," is being conducted in Australia, Canada, and Aotearoa/New Zealand. OBJECTIVE: To conduct the research in New Zealand using a kaupapa Māori (Māori philosophy) approach. METHODS: This is a mixed-method study incorporating quantitative and qualitative data whilst acknowledging Māori cultural practices by the utilization of Te Whare Tapa Whā, a model for Māori health and well-being...
2016: Journal of Health Care for the Poor and Underserved
Theresia M Westers, Eline M P Cremers, Uta Oelschlaegel, Ulrika Johansson, Peter Bettelheim, Sergio Matarraz, Alberto Orfao, Bijan Moshaver, Lisa Eidenschink Brodersen, Michael R Loken, Denise A Wells, Dolores Subira', Matthew Cullen, Jeroen G Te Marvelde, Vincent H J Van der Velden, Frank W M B Preijers, Sung-Chao Chu, Jean Feuillard, Estelle Guérin, Katherina Psarra, Anna Porwit, Leonie Saft, Robin Ireland, Timothy Milne, Marie C Béné, Birgit I Witte, Matteo G Della Porta, Wolfgang Kern, Arjan A van de Loosdrecht
Current recommendations for diagnosing myelodysplastic syndromes endorse flow cytometry as an informative tool. Most flow cytometry protocols focus on the analysis of progenitor cells and the evaluation of the maturing myelomonocytic lineage. However, one of the most frequently observed features of myelodysplastic syndromes is anemia, which may be associated with dyserythropoiesis. Therefore, analysis of changes in flow cytometry features of nucleated erythroid cells may complement current flow cytometry tools...
October 6, 2016: Haematologica
Norbert Garcia-Henriquez, Eric M Toloza, Farah Khalil, Maria F Echavarria, Joseph R Garrett, Carla C Moodie, Frank J Kaszuba, Jacques P Fontaine
We present the case of a 55-year-old man who developed extensive occlusive bronchial casts after trachea-esophageal (TE) fistula repair. The bronchial casts were treated by bronchoscopic extraction, high dose steroids, antibiotics, and antifungals. Despite this multi-modality treatment, the rapid formation of these occlusive bronchial casts was very aggressive and could not be controlled even with a series of five rigid bronchoscopic extractions within a 48-hour period. The patient quickly deteriorated and succumbed to the inflammatory state...
September 2016: Journal of Thoracic Disease
Shu Harn Te, Boon Fei Tan, Janelle R Thompson, Karina Yew-Hoong Gin
Genomes of two filamentous benthic cyanobacteria were obtained from cocultures obtained from two freshwater lakes. The cultures were obtained by first growing cyanobacterial trichome on solid medium, followed by subculturing in freshwater media. Subsequent shotgun sequencing, de novo assembly, and genomic binning yielded almost complete genomes of Oscillatoriales USR 001 and Nostoc sp. MBR 210.
October 13, 2016: Genome Announcements
Joyce Kelly R da Silva, Laine C Pinto, Rommel M R Burbano, Raquel C Montenegro, Eloísa Helena A Andrade, José Guilherme S Maia
BACKGROUND: Piper aequale Vahl is a small shrub that grows in the shadow of large trees in the Carajás National Forest, Municipality of Parauapebas, Para state, Brazil. The local people have used the plant against rheumatism and inflammation. METHODS: The essential oil of the aerial parts was extracted and analyzed by GC and GC-MS. The MTT colorimetric assay was used to measuring the cytotoxic activity of the oil against human cancer lines. The determination of antioxidant activity of the oil was conducted by DPPH radical scavenging assay...
October 7, 2016: Lipids in Health and Disease
Douglas R Marques, Luís A L Dos Santos, Marie A O'Brien, Sarah H Cartmell, Julie E Gough
The polymeric blend of poly (lactic-co-glycolic acid) (PLGA) and polyisoprene (PI) has recently been explored for application as stents for tracheal stenosis and spring for the treatment of craniosynostosis. From the positive results presented in other biomedical applications comes the possibility of investigating the application of this material as scaffold for tissue engineering (TE), acquiring a deeper knowledge about the polymeric blend by exploring a new processing technique while attending to the most fundamental demands of TE scaffolds...
October 6, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Yi Du, Hirohito Yamaguchi, Yongkun Wei, Jennifer L Hsu, Hung-Ling Wang, Yi-Hsin Hsu, Wan-Chi Lin, Wen-Hsuan Yu, Paul G Leonard, Gilbert R Lee, Mei-Kuang Chen, Katsuya Nakai, Ming-Chuan Hsu, Chun-Te Chen, Ye Sun, Yun Wu, Wei-Chao Chang, Wen-Chien Huang, Chien-Liang Liu, Yuan-Ching Chang, Chung-Hsuan Chen, Morag Park, Philip Jones, Gabriel N Hortobagyi, Mien-Chie Hung
No abstract text is available yet for this article.
October 6, 2016: Nature Medicine
R C R Noronha, L M R Barros, R E F Araújo, D F Marques, C Y Nagamachi, C Martins, J C Pieczarka
BACKGROUND: Cytogenetic studies were conducted in the Brazilian Amazon turtles, Podocnemis expansa Schweigger, 1912 (PEX) and Podocnemis unifilis Troschel, 1848 (PUN) to understand their karyoevolution. Their chromosomal complements were compared using banding techniques (C, G-, Ag-NOR and Chromomycin A3) and fluorescence in situ hybridization (FISH), and efforts were made to establish evolutionary chromosomal relationships within the Podocnemidae family. RESULTS: Our results revealed that both species have a chromosome complement of 2n = 28...
2016: Molecular Cytogenetics
Michael E J Lean, Lisa Te Morenga
BACKGROUND: Consumption of sugar, specifically sugar-sweetened beverages, has been widely held responsible by the media for the global rise in Type 2 diabetes (T2DM). SOURCES OF DATA: Systematic reviews and dietary guidelines relating dietary sugars to T2DM. AREAS OF AGREEMENT: Weight gain and T2DM incidence are associated with diet and lifestyle patterns characterized by high consumptions of any sweetened beverages. High sugar intakes impair risk factors for macrovascular complications of T2DM...
October 5, 2016: British Medical Bulletin
M Huang, X J Sun, Y Zhou, X M Wang
In this study, we compared the quality of DNA extracted using the modified CTAB method, from Rheum palmatum leaves preserved using fourteen different methods, including ones used commonly in other species: under ultra-cold (-80°C) temperatures, after drying with an absorbent paper, desiccating using a silica gel, drying at 60°C, in 70% ethanol, absolute ethanol, 70% ethanol supplemented with 50 mM EDTA, SDS-DNA extracting solution, nuclear separation buffer, improved NaCl-CTAB solution, TE-buffer, I-solution, or II-solution...
August 19, 2016: Genetics and Molecular Research: GMR
Ericky C A Araujo, N Azzabou, A Vignaud, G Guillot, P G Carlier
PURPOSE: To introduce an ultrashort echo time (UTE) based method for quantitative mapping of short-T2 signals in skeletal muscle (SKM) in the presence of fat, with the aim of monitoring SKM fibrosis. METHODS: From a set of at least five UTE images of the same slice, a long- T2* map, a fat-fraction map, and a map of short-T2 -signal fraction are extracted. The method was validated by numerical simulations and in vitro studies on collagen solutions. Finaly, the method was applied to image the short-T2 signals in the leg of eight healthy volunteers...
October 3, 2016: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
K Yasuda, A Tsukazaki, R Yoshimi, K S Takahashi, M Kawasaki, Y Tokura
We report current-direction dependent or unidirectional magnetoresistance (UMR) in magnetic or nonmagnetic topological insulator (TI) heterostructures, Cr_{x}(Bi_{1-y}Sb_{y})_{2-x}Te_{3}/(Bi_{1-y}Sb_{y})_{2}Te_{3}, that is several orders of magnitude larger than in other reported systems. From the magnetic field and temperature dependence, the UMR is identified to originate from the asymmetric scattering of electrons by magnons. In particular, the large magnitude of UMR is an outcome of spin-momentum locking and a small Fermi wave number at the surface of TI...
September 16, 2016: Physical Review Letters
Abdolreza Yazdani, Holly Bilton, Alyssa Vito, Afaf R Genady, Stephanie M Rathmann, Zainab Ahmad, Nancy Janzen, Shannon Czorny, Brian M Zeglis, Lynn C Francesconi, John F Valliant
A high yield synthesis of a novel, small molecule, bisphosphonate-modified trans-cyclooctene (TCO-BP, 2) that binds to regions of active bone metabolism and captures functionalized tetrazines in vivo, via the bioorthogonal inverse electron demand Diels-Alder (IEDDA) cycloaddition, was developed. A (99m)Tc-labeled derivative of 2 demonstrated selective localization to shoulder and knee joints in a biodistribution study in normal mice. Compound 2 reacted rapidly with a (177)Lu-labeled tetrazine in vitro, and pretargeting experiments in mice, using 2 and the (177)Lu-labeled tetrazine, yielded high activity concentrations in shoulder and knee joints, with minimal uptake in other tissues...
October 5, 2016: Journal of Medicinal Chemistry
T Brazdauskas, L Montero, P R Venskutonis, E Ibañez, M Herrero
In this work, a new alternative for the downstream processing and valorization of black chokeberry pomace (Aronia melanocarpa) which could be potentially coupled to a biorefinery process is proposed. This alternative is based on the application of pressurized liquid extraction (PLE) to the residue obtained after the supercritical fluid extraction of the berry pomace. An experimental design is employed to study and optimize the most relevant extraction conditions in order to attain extracts with high extraction yields, total phenols content and antioxidant activity...
October 14, 2016: Journal of Chromatography. A
Nirav Pipaliya, Dattatray Solanke, Pathik Parikh, Meghraj Ingle, Ratna Sharma, Sujata Sharma, Prabha Sawant
BACKGROUND & AIMS: We investigated whether tissue elastography (TE) can be used as an alternative to magnetic resonance imaging (MRI) T2* analysis to determine the degree of iron overload in patients with thalassemia major. METHODS: We conducted a prospective study 154 patients (99 male; mean age, 12±3.6 years) with thalassemia major requiring chronic blood transfusion and on iron chelator therapy. The study was performed at a tertiary hospital in India from January 2015 through June 2015...
September 17, 2016: Clinical Gastroenterology and Hepatology
Chu-Ning Huang, Chan-Pin Lin, Feng-Chia Hsieh, Sook-Kuan Lee, Kuan-Chen Cheng, Chi-Te Liu
Bacillus amyloliquefaciens strain WF02, isolated from soil collected at Wufeng Mountain, Taiwan, has siderophore-producing ability and in vitro antagonistic activity against bacterial wilt pathogen. To determine the impact of plant genotype on biocontrol effectiveness, we treated soil with this strain before infecting susceptible (L390) and moderately resistant (Micro-Tom) tomato cultivars with Ralstonia solanacearum strain Pss4. We also compared the efficacy of this strain with that of commercial Bacillus subtilis strain Y1336...
November 2016: World Journal of Microbiology & Biotechnology
H E Dekter, C C Orelio, M C Morsink, S Tektas, B Vis, R Te Witt, W B van Leeuwen
Patients suffering from bacterial bloodstream infections have an increased risk of developing systematic inflammatory response syndrome (SIRS), which can result in rapid deterioration of the patients' health. Diagnostic methods for bacterial identification and antimicrobial susceptibility tests are time-consuming. The aim of this study was to investigate whether Raman spectroscopy would be able to rapidly provide an antimicrobial susceptibility profile from bacteria isolated directly from positive blood cultures...
September 8, 2016: European Journal of Clinical Microbiology & Infectious Diseases
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