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Seung Jae Lee, Eun-Mi Jeong, Ah Young Ki, Kyung-Seo Oh, Joseph Kwon, Jae-Hyuk Jeong, Nam-Jin Chung
High salinity is a major abiotic stress that affects the growth and development of plants. This type of stress can influence flowering, the production of crops, defense mechanisms and other physiological processes. Previous studies have attempted to elucidate salt-tolerance mechanisms to improve plant growth and productivity in the presence of sodium chloride. One such plant that has been studied in detail is Salicornia, a well-known halophyte, which has adapted to grow in the presence of high salt. To further the understanding of how Salicornia grows and develops under high saline conditions, Salicornia herbacea (S...
September 29, 2016: Journal of Plant Physiology
Macc Rigdon, Yen-Con Hung, Alexander M Stelzleni
Sixty-four pork loins were randomly assigned to one of four treatments to evaluate the use of alkaline electrolyzed reduced water as a replacement for traditional enhancement solutions. Treatments included: alkaline electrolyzed reduced water (EOH; pH≈11.5), EOH plus 2.5% potassium-lactate (EOK), industry standard (IS; 0.35% sodium tri-polyphosphate, 0.14% sodium chloride, 2.5% potassium-lactate), and no enhancement (CON). After enhancement (targeting 110%) and rest period, chops were cut (2.54-cm) to test treatment effects on water holding capacity, Warner-Bratzler shear force (WBSF), and sensory attributes...
October 14, 2016: Meat Science
Narendra Kumar, Vijay Kumar, Rohit Panwar, Chand Ram
The toxic heavy metal cadmium (Cd) appears as one of the major global threats to human and animal health. Human being and aquatic life are exposed to Cd by breathing, eating, or drinking when industrial effluents released into environment. The study was aimed to identify cadmium-binding Lactobacillus strain to reduce its bioaccessibility in in vitro digestion model. In this context, forty-eight lactobacilli strains isolated and characterized from fermented dairy products and human origin were screened for their Cd biosorption potential using Flame Atomic Absorption Spectroscopy (FAAS)...
October 21, 2016: Environmental Science and Pollution Research International
Zhongbing Chen, Jan Vymazal, Peter Kuschk
Three different flow regimes were carried out in a pilot-scale horizontal subsurface flow constructed wetland-treating sulfate rich wastewater contaminated with monochlorobenzene (MCB) and perchloroethene (PCE). The three regimes were continuous flow, 7-day cycle discontinuous flow, and 2.5-day cycle discontinuous flow. The results show that intensifying the tidal regime (2.5-day cycle) significantly enhanced MCB removal before 2 m from the inlet and increasing PCE removal efficiency at 0.5 m. The PCE dechlorination process was promoted with tidal operation, especially under the 2...
October 21, 2016: Environmental Science and Pollution Research International
Islam Ghazi, Yukihiro Hamada, David P Nicolau
PURPOSE: The physical compatibility of commonly used agents that could be coadministered in the clinical setting with tedizolid phosphate during Y-site administration was evaluated. METHODS: Tedizolid phosphate vials were reconstituted to a final concentration of 0.8 mg/mL. All other drugs were prepared according to manufacturers' recommendations and diluted with 0.9% sodium chloride injection (where applicable) to the highest standard concentrations used clinically...
November 1, 2016: American Journal of Health-system Pharmacy: AJHP
Gabriela Debom, Marta Gazal, Mayara Sandrielly Pereira Soares, Carlus Augustu Tavares do Couto, Bruna Mattos, Claiton Lencina, Manuella Pinto Kaster, Gabriele Codenonzi Ghisleni, Rejane Tavares, Elizandra Braganhol, Vitor Clasen Chaves, Flávio Henrique Reginatto, Francieli Stefanello, Roselia Maria Spanevello
The aim of the present study was to evaluate the protective effects of blueberry extract on oxidative stress and inflammatory parameters in a model of mania induced by ketamine administration in rats. Male rats were pretreated with blueberry extract (200mg/kg, once a day for 14 days) lithium chloride (45mg/kg, mood stabilizer used as a positive control, twice a day for 14 days), or vehicle. Between the 8th and 14th days, rats also received an injection of ketamine (25mg/kg) or vehicle. In the 15th day, thirty minutes after ketamine administration the hyperlocomotion of the animals was assessed in the open - field apparatus...
October 18, 2016: Brain Research Bulletin
Stephanie Lemos Martins Sicuro, Marilisa Carneiro Leão Gabardo, Carla Castiglia Gonzaga, Nathaly Dias Morais, Flares Baratto-Filho, Gisele Maria Correr Nolasco, Denise Piotto Leonardi
INTRODUCTION: Different materials have been used for intervening in root perforations. These materials are often in contact with resin cements used for cementation of intraradicular retainers. The aim of this study was to evaluate the bond strength of self-adhesive resin cement to different materials used to treat root perforations (mineral trioxide aggregate [MTA], Portland cement [PORT], and glass ionomer cement [GIC]). METHODS: Four discs (10 × 1 mm) of each material (the MTA, PORT, and GIC groups) were embedded into polyvinyl chloride tubes using acrylic resin, ground, and polished until a flat surface was exposed...
October 18, 2016: Journal of Endodontics
Susumu Ishiguro, Nabil A Alhakamy, Deepthi Uppalapati, Jennifer Delzeit, Cory J Berkland, Masaaki Tamura
To evaluate the potential of cell-penetrating peptide-based delivery of apoptosis-inducer gene in cancer therapy, a modified HIV-1 TAT peptide (dimerized TAT peptide, dTAT) was studied. The dTAT and plasmid DNA (pDNA) complexes (dTAT-pDNA) were condensed using calcium chloride (dTAT-pDNA-Ca(2+)). This simple nonviral formulation approach showed high levels of gene expression in vitro without any cytotoxicity. In mouse studies, a single intratracheal (IT) aerosol spray or 2 intravenous (IV) injections of the dTAT, apoptosis-inducer gene, angiotensin II type 2 receptor (AT2R), and Ca(2+) complexes (dTAT-pAT2R-Ca(2+)) significantly attenuated the acutely growing mouse Lewis lung carcinoma allografts in mouse lungs...
October 18, 2016: Journal of Pharmaceutical Sciences
Huichao Li, Lu Wang, Kai Sheng, Lina Zou, Baoxian Ye
The nanocomposites of titanium dioxide nanoparticles decorated poly(diallyldimethylammonium chloride)-functionalized graphene (TiO2-PDDA-Gr) were successfully synthesized via a facile and green strategy. The prepared nanocomposites were characterized by X-ray diffraction (XRD), UV-visible spectrophotometry (UV-vis), Fourier Transform infrared spectroscopy (FT-IR) and transmission electron microscopy (TEM). By combining the merits of the PDDA-Gr and TiO2 NPs, a highly sensitive electrochemical sensor was erected to detect esculetin based on the TiO2-PDDA-Gr nanocomposites...
December 1, 2016: Talanta
Cihan Topcu
A novel polyvinyl chloride membrane chlorate (ClO3(-)) selective electrode based on modified smectite was developed for the direct determination of chlorate ions and the potentiometric performance characteristics of its were examined. The best selectivity and sensitivity for chlorate ions were obtained for the electrode membrane containing ionophore/polyvinylchloride/o-nitrophenyloctylether in composition of 12/28/60 (w/w%). The proposed electrode showed a Nernstian response toward chlorate ions at pH=7 in the concentration range of 1×10(-7)-1×10(-1)M and the limit of detection was calculated as 9×10(-8)M from the constructed response plot...
December 1, 2016: Talanta
Xuan Zhang, Yi-Chi Zhang, Jia-Wei Zhang
Chloramphenicol (CAP), as a broad-spectrum antibiotic, has been worldwide banned for using in the food producing animals due to its overuse may cause severe threats to public health. It is therefore highly desirable to develop facile, selective and sensitive biosensor for CAP detection and monitoring in drug and foodstuff samples. In this work, three-dimensional reduced graphene oxide (3DRGO) architectures were prepared through a green and template-free approach and used as active electrode materials to develop a highly selective electrochemical sensor for CAP detection...
December 1, 2016: Talanta
Zhigang Sui, Yejing Weng, Qun Zhao, Nan Deng, Fei Fang, Xudong Zhu, Yichu Shan, Lihua Zhang, Yukui Zhang
The cartilage zone of the velvet antler is richly vascularized, this being a major difference to the classical cartilage, in which there are no blood vessels. Angiogenesis and rapid growth of vasculature in velvet antler cartilage (VAC) make it an ideal model for discovering the novel angiogenic regulatory factors. However, the proteomic analysis of VAC is challenging due to the serious interference of proteoglycans (PGs) and collagens. To achieve a comprehensive proteome characterization of VAC, herein, we developed an ionic liquid-based method using 1-dodecyl-3-methylimidazolium chloride ([C12-mim]Cl) for selective extraction of cellular proteins from VAC...
December 1, 2016: Talanta
Mohsen Asadnia, Matthew Myers, N D Akhavan, Kane O'Donnell, Gilberto A Umana-Membreno, U K Mishra, Brett Nener, Murray Baker, Giacinta Parish
This work presents the first polymer approach to detect metal ions using AlGaN/GaN transistor-based sensor. The sensor utilised an AlGaN/GaN high electron mobility transistor-type structure by functionalising the gate area with a polyvinyl chloride (PVC) based ion selective membrane. Sensors based on this technology are portable, robust and typically highly sensitive to the target analyte; in this case Hg(2+). This sensor showed a rapid and stable response when it was introduced to solutions of varying Hg(2+) concentrations...
November 2, 2016: Analytica Chimica Acta
Tommaso Michele Moles, Antonio Pompeiano, Thais Huarancca Reyes, Andrea Scartazza, Lorenzo Guglielminetti
Landraces represent an important part of the biodiversity well-adapted under limiting environmental conditions. We investigated the response of two Southern Italy tomato landraces, the well-known San Marzano (our commercial standard) and a local accession called "Ciettaicale", to different levels of sodium chloride in water irrigation (from 0 up to 600 mM) for a short-time exposure (one week). The combination of the chlorophyll a fluorescence and gas exchange analyses suggested that Ciettaicale maintained a higher efficiency of photosystem II (PSII) photochemistry and CO2 utilization at high salinity concentrations than San Marzano...
October 12, 2016: Plant Physiology and Biochemistry: PPB
Kirilka Mladenova, Svetla D Petrova, Tonya D Andreeva, Veselina Moskova-Doumanova, Tanya Topouzova-Hristova, Yuri Kalvachev, Konstantin Balashev, Shomi S Bhattacharya, Christina Chakarova, Zdravko Lalchev, Jordan A Doumanov
Human bestrophin-1 (hBest1) is a transmembrane calcium-activated chloride channel protein - member of the bestrophin family of anion channels, predominantly expressed in the membrane of retinal pigment epithelium (RPE) cells. Mutations in the protein cause ocular diseases, named Bestrophinopathies. Here, we present the first Fourier transform infrared (FTIR) study of the secondary structure elements of hBest1, π/A isotherms and hysteresis, Brewster angle microscopy (BAM) and atomic force microscopy (AFM) visualization of the aggregation state of protein molecules dispersed as Langmuir and Langmuir-Blodgett films...
October 13, 2016: Colloids and Surfaces. B, Biointerfaces
Qian Lu, Jihong Wang, Junshu Jiang, Shengnan Wang, Qilan Jia, Yue Wang, Weiping Li, Qin Zhou, Li Lv, Qingwei Li
BACKGROUND: The RGD-toxin protein Lj-RGD3 is a naturally occurring 118 amino acid peptide that can be obtained from the salivary gland of the Lampetra japonica fish. This unique peptide contains 3 RGD (Arg-Gly-Asp) motifs in its primary structure. Lj-RGD3 is available in recombinant form (rLj-RGD3) and can be produced in large quantities using DNA recombination techniques. The pharmacology of the three RGD motif-containing peptides has not been studied. This study investigated the protective effects of rLj-RGD3, a novel polypeptide, against ischemia/reperfusion-induced damage to the brain caused by middle cerebral artery occlusion (MCAO) in a rat stroke model...
2016: PloS One
Yuji Kaneko, Colleen Pappas, Naoki Tajiri, Cesar V Borlongan
Oxytocin protects against ischemia-induced inflammation and oxidative stress, and is associated with GABA (γ-aminobutyric acid, an inhibitory neurotransmitter) signaling transduction in neurons. However, the molecular mechanism by which oxytocin affords neuroprotection, especially the interaction between oxytocin receptor and GABAA receptor (GABAAR), remains to be elucidated. Primary rat neural cells were exposed to oxytocin before induction of experimental acute stroke model via oxygen-glucose deprivation-reperfusion (OGD/R) injury...
October 21, 2016: Scientific Reports
Agostino Biafora, Thilo Krause, Dagmar Hackenberger, Florian Belitz, Lukas J Gooßen
A system consisting of catalytic amounts of [(p-cym)RuCl2 ]2 /PEt3 ⋅HBF4 , K2 CO3 as the base, and NMP as the solvent efficiently mediates the ortho-C-H arylation of benzoic acids with aryl bromides at 100 °C. Replacing the phosphine ligand with the amino acid dl-pipecolinic acid enables the analogous transformation with aryl chlorides. The key advantage of this broadly applicable transformation is the use of an inexpensive ruthenium catalyst in combination with simple carboxylates as directing groups, which can either be tracelessly removed or used as anchor points for decarboxylative ipso substitutions...
October 21, 2016: Angewandte Chemie
Liang Liu, Junnian Wei, Yue Chi, Wen-Xiong Zhang, Zhenfeng Xi
The chemistry of magnesium organocuprates, including their synthesis, structures, and reactions, remains underexplored. In this work, by taking advantage of the high reactivity and ready availability of magnesiacyclopentadienes, a series of magnesiacyclopentadiene-based organocuprates were synthesized and structurally characterized. A variety of CuX salts (X=Cl, Br, I, or alkynyl) were successfully applied to react with magnesiacyclopentadienes. Besides CuX salts, AgX salts (X=Cl, alkynyl) also undergwent the above reaction to afford the corresponding magnesium organoargentates...
October 21, 2016: Angewandte Chemie
Sunday A Adebusoye
Cupriavidus sp. strain SK-3, previously isolated on polychlorinated biphenyl mixtures, was found to aerobically utilize a wide spectrum of substituted aromatic compounds including 4-fluoro-, 4-chloro- and 4-bromobenzoic acids as a sole carbon and energy source. Other chlorobenzoic acid (CBA) congeners such as 2-, 3-, 2,3-, 2,5-, 3,4- and 3,5-CBA were all rapidly transformed to respective chlorocatechols (CCs). Under aerobic conditions, strain SK-3 grew readily on 4-CBA to a maximum concentration of 5 mM above which growth became impaired and yielded no biomass...
October 20, 2016: Biodegradation
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