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Formaldehyde

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https://www.readbyqxmd.com/read/29356267/cleavable-crosslinkers-as-tissue-fixation-reagents-for-proteomic-analysis
#1
Sara Ongay, Miriam Langelaar-Makkinje, Marcel P Stoop, Nora Liu, Hermen Overkleeft, Theo M Luider, Geny M M Groothuis, Rainer Bischoff
Formaldehyde fixation is widely used for long-term storage of tissue. However, due to formaldehyde-induced cross-links, fixated tissue proteins are difficult to extract hampering mass spectrometry (MS)-proteomic analyses. In the present work we evaluate cleavable protein crosslinkers as fixatives that allow tissue preservation as well as efficient protein extraction for MS-proteomics under mild conditions. We investigated disuccinimidyl tartrate (DST) and dithiobis[succinimidylpropionate] (DSP) as cleavable fixating reagents...
January 22, 2018: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/29354896/-10-year-series-studies-of-chronic-radiation-proctopathy-from-the-sixth-affiliated-hospital-of-sun-yat-sen-university
#2
Lei Wang, Tenghui Ma, Jianping Wang
Chronic radiation proctopathy (CRP) is the delayed adverse effect after radiotherapy for pelvic malignancies. It is characterized as progressive fibrosis in pathology. The symptoms are usually unspecific which include rectal bleeding, perianal pain, tenesmus, stool frequencies and so on. The course of CRP can present from months to years after radiation. Severe complications can occur in the late stage and will greatly affect the quality of life. Currently, there is no standard guides for diagnosis and treatment of CRP...
January 25, 2018: Zhonghua Wei Chang Wai Ke za Zhi, Chinese Journal of Gastrointestinal Surgery
https://www.readbyqxmd.com/read/29350520/one-step-synthesis-of-co-doped-in2o3-nanorods-for-high-response-of-formaldehyde-sensor-at-low-temperature
#3
Zhihua Wang, Changliang Hou, Qinma De, Fubo Gu, Dongmei Han
Uniform and monodisperse Co-doped In2O3 nanorods were fabricated by a facile and environmentally friendly hydrothermal strategy that combined the subsequent annealing process, and their morphology, structure and formaldehyde (HCHO) gas sensing performance were investigated systematically. Both pure and Co-doped In2O3 nanorods had a high specific surface area, which could offer abundant reaction sites to gas molecular diffusion and improve the response of gas sensor. Results revealed that the In2O3/1%Co nanorods exhibited a higher response of 23...
January 19, 2018: ACS Sensors
https://www.readbyqxmd.com/read/29347944/expression-of-ugp2-and-cfl1-expression-levels-in-benign-and-malignant-pancreatic-lesions-and-their-clinicopathological-significance
#4
Lingxiang Wang, Li Xiong, Zhengchun Wu, Xiongying Miao, Ziru Liu, Daiqiang Li, Qiong Zou, Zhulin Yang
BACKGROUND: This study investigated UGP2 (uridine diphosphate-glucose pyrophosphorylase-2) and CFL1 (cofilin-1) expression in pancreatic ductal carcinoma (PDC), paracancerous tissue (PT), benign lesions (BL), and normal tissue (NT) and their clinicopathological significance. METHODS: Surgical specimens, which were collected from 106 cases of pancreatic ductal carcinoma, 35 cases of paracancerous tissues, 55 cases of benign lesions and 13 cases of normal pancreatic tissues, were fixed with 4% formaldehyde to prepare conventional paraffin-embedded sections...
January 18, 2018: World Journal of Surgical Oncology
https://www.readbyqxmd.com/read/29345378/self-calibrating-mechanochromic-fluorescent-polymers-based-on-encapsulated-excimer-forming-dyes
#5
Céline Calvino, Anirvan Guha, Christoph Weder, Stephen Schrettl
While mechanochemical transduction principles are omnipresent in nature, mimicking these in artificial materials is challenging. The ability to reliably detect the exposure of man-made objects to mechanical forces is, however, of great interest for many applications, including structural health monitoring and tamper-proof packaging. A useful concept to achieve mechanochromic responses in polymers is the integration of microcapsules, which rupture upon deformation and release a payload causing a visually detectable response...
January 18, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29344904/mgmt-assessment-in-pituitary-adenomas-comparison-of-different-immunohistochemistry-fixation-chemicals
#6
Alexander S G Micko, Romana Höftberger, Adelheid Wöhrer, Matthias Millesi, Engelbert Knosp, Stefan Wolfsberger
PURPOSE: Despite the established role of O6-methyl-guanine-DNA methyltransferase (MGMT) as a marker for temozolomide response, consensus of the most reliable method to assess MGMT expression in pituitary adenomas is still missing. Currently, immunohistochemistry (IHC) assessment of formaldehyde fixed tissue samples is most widely used in a semiquantitative description. As formaldehyde fails to completely preserve nucleic acids, RCL2, an alcohol-based formaldehyde-free fixative, has been proposed as a more reliable alternative in terms of cell stability...
January 17, 2018: Pituitary
https://www.readbyqxmd.com/read/29341859/an-indoor-air-quality-evaluation-in-a-residential-retrofit-project-using-spray-polyurethane-foam
#7
Shen Tian, Scott Ecoff, John Sebroski, Jason Miller, Harold Rickenbacker, Melissa Bilec
Understanding of indoor air quality (IAQ) during and after spray polyurethane foam (SPF) application is essential to protect the health of both workers and building occupants. Previous efforts such as field monitoring, micro-chamber/spray booth emission studies and fate/transport modeling have been conducted to understand the chemical exposure of SPF and guide risk mitigation strategies. However, each type of research has its limitation and can only reveal partial information on the relationship between SPF and IAQ...
January 17, 2018: Journal of Occupational and Environmental Hygiene
https://www.readbyqxmd.com/read/29341499/recommendations-on-diagnostic-tools-for-batrachochytrium-salamandrivorans
#8
V Thomas, M Blooi, P Van Rooij, S Van Praet, E Verbrugghe, E Grasselli, M Lukac, S Smith, F Pasmans, A Martel
Batrachochytrium salamandrivorans (Bsal) poses a major threat to amphibian, and more specifically caudata, diversity. Bsal is currently spreading through Europe, and mitigation measures aimed at stopping its spread and preventing its introduction into naïve environments are urgently needed. Screening for presence of Bsal and diagnosis of Bsal-induced disease in amphibians are essential core components of effective mitigation plans. Therefore, the aim of this study was to present an overview of all Bsal diagnostic tools together with their limitations and to suggest guidelines to allow uniform interpretation...
January 17, 2018: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29339722/the-deep-subsurface-sulfate-reducer-desulfotomaculum-kuznetsovii-employs-two-methanol-degrading-pathways
#9
Diana Z Sousa, Michael Visser, Antonie H van Gelder, Sjef Boeren, Mervin M Pieterse, Martijn W H Pinkse, Peter D E M Verhaert, Carsten Vogt, Steffi Franke, Steffen Kümmel, Alfons J M Stams
Methanol is generally metabolized through a pathway initiated by a cobalamine-containing methanol methyltransferase by anaerobic methylotrophs (such as methanogens and acetogens), or through oxidation to formaldehyde using a methanol dehydrogenase by aerobes. Methanol is an important substrate in deep-subsurface environments, where thermophilic sulfate-reducing bacteria of the genus Desulfotomaculum have key roles. Here, we study the methanol metabolism of Desulfotomaculum kuznetsovii strain 17T, isolated from a 3000-m deep geothermal water reservoir...
January 16, 2018: Nature Communications
https://www.readbyqxmd.com/read/29338790/morphometric-characterisation-of-human-tracheas-focus-on-cartilaginous-ring-variation
#10
Y Premakumar, M F Griffin, M Szarko
PURPOSE: Details regarding tracheal anatomy are currently lacking, with existing literature focussing mainly on the cricoid-tracheal region or the carina. External gross anatomy and internal morphology throughout the entire trachea is important for normal physiological functioning and various clinical applications such as designs for tracheal implants or endotracheal devices. OBJECTIVE: To determine quantitative and qualitative characteristics of gross tracheal and individual tracheal ring anatomy...
January 16, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/29332788/turn-on-fluorescent-sensor-for-the-detection-of-glucose-using-manganese-dioxide-phenol-formaldehyde-resin-nanocomposite
#11
Zhong Feng Gao, Asmerom Yohannes Ogbe, Ei Ei Sann, Xudong Wang, Fan Xia
Monitoring blood glucose has attracted considerable attention because diabetes mellitus is a global public health problem. Herein, we reported a turn-on fluorescence detection strategy based on manganese dioxide (MnO2)-phenol formaldehyde resin (PFR) nanocomposite for rapid, sensitive, and selective detection of glucose levels in human blood. In this biosensing system, MnO2 nanoshell on the PFR nanoparticle surfaces serve as a quencher. PFR fluorescence can make a recovery in the presence of H2O2, reducing MnO2 to Mn2+...
April 1, 2018: Talanta
https://www.readbyqxmd.com/read/29330529/the-interaction-network-of-the-yidc-insertase-with-the-secyeg-translocon-srp-and-the-srp-receptor-ftsy
#12
Narcis-Adrian Petriman, Benjamin Jauß, Antonia Hufnagel, Lisa Franz, Ilie Sachelaru, Friedel Drepper, Bettina Warscheid, Hans-Georg Koch
YidC/Oxa1/Alb3 are essential proteins that operate independently or cooperatively with the Sec machinery during membrane protein insertion in bacteria, archaea and eukaryotic organelles. Although the interaction between the bacterial SecYEG translocon and YidC has been observed in multiple studies, it is still unknown which domains of YidC are in contact with the SecYEG translocon. By in vivo and in vitro site-directed and para-formaldehyde cross-linking we identified the auxiliary transmembrane domain 1 of E...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29326091/erratum-regulation-of-chromatin-assembly-and-cell-transformation-by-formaldehyde-exposure-in-human-cells
#13
Danqi Chen, Lei Fang, Shenglin Mei, Hongjie Li, Xia Xu, Thomas L Des Marais, Kun Lu, X Shirley Liu, Chunyuan Jin
[This corrects the article DOI: 10.1289/EHP1275.].
January 3, 2018: Environmental Health Perspectives
https://www.readbyqxmd.com/read/29324749/brand-switching-and-toxic-chemicals-in-cigarette-smoke-a-national-study
#14
Jennifer R Mendel, Sabeeh A Baig, Marissa G Hall, Michelle Jeong, M Justin Byron, Jennifer C Morgan, Seth M Noar, Kurt M Ribisl, Noel T Brewer
INTRODUCTION: US law requires disclosure of quantities of toxic chemicals (constituents) in cigarette smoke by brand and sub-brand. This information may drive smokers to switch to cigarettes with lower chemical quantities, under the misperception that doing so can reduce health risk. We sought to understand past brand-switching behavior and whether learning about specific chemicals in cigarette smoke increases susceptibility to brand switching. METHODS: Participants were US adult smokers surveyed by phone (n = 1,151, probability sample) and online (n = 1,561, convenience sample)...
2018: PloS One
https://www.readbyqxmd.com/read/29324378/impact-evaluation-of-environmental-factors-on-respiratory-function-of-asthma-patients-living-in-urban-territory
#15
Lyudmila V Veremchuk, Konstantinos Tsarouhas, Tatyana I Vitkina, Elena E Mineeva, Tatyana A Gvozdenko, Marina V Antonyuk, Valeri N Rakitskii, Karolina A Sidletskaya, Aristidis M Tsatsakis, Kirill S Golokhvast
BACKGROUND: Environmental pollution, local climatic conditions and their association with the prevalence and exacerbation of asthma are topics of intense current medical investigation. MATERIALS AND METHODS: Air pollution in the area of Vladivostock was estimated both by the index of emission volumes of "air gaseous components" (nitrogen oxide and nitrogen dioxide, formaldehyde, hydrogen sulfide, carbon monoxide) in urban atmosphere and by mass spectrometric analysis of precipitates in snow samples...
January 8, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29323422/community-interest-and-feasibility-of-using-a-novel-smartphone-based-formaldehyde-exposure-detection-technology
#16
Jessica Castner, Gretchen E Gehrke, Nicholas Shapiro, Karen C Dannemiller
OBJECTIVE: This study is the first community engagement phase of a project to develop a residential formaldehyde detection system. The objectives were to conduct a feasibility assessment for device use, and identify factors associated with concerns about environmental exposure and community interest in this device. DESIGN AND SAMPLE: A cross-sectional, internet-based survey employing community-based participatory research principles was utilized. 147 individuals participated from a focused Waycross, Georgia (58...
January 11, 2018: Public Health Nursing
https://www.readbyqxmd.com/read/29322584/effects-of-fixation-on-electrophysiology-and-structure-of-human-jejunal-villi
#17
Anna Casselbrant, Herbert F Helander
The villi of human jejunum vary in size and shape during different functional conditions. In the base the lamina propria is isotonic with blood, in the tip hyperosmotic. Here we study electrophysiological and morphological effects of incubation in hypotonic, isotonic, or hypertonic solutions, and to test various isotonic fixatives for microscopy. Samples of jejunal mucosae, obtained during surgery in obese patients, were studied in Ussing chambers where electrical parameters were registered during incubation in Krebs solution at various osmolarities, and during fixation in formaldehyde, glutaraldehyde, or osmium tetroxide (OsO4 )...
January 11, 2018: Microscopy Research and Technique
https://www.readbyqxmd.com/read/29322416/tissue-processing-for-stereological-analyses-of-lung-structure-in-chronic-obstructive-pulmonary-disease
#18
Mohamed Saad, Saleela M Ruwanpura
Quantitative data on lung structure, such as volume, surface area and length, are used for assessment of the functional performance of the lung during normal development and inflammatory-related diseases such as chronic obstructive pulmonary disorder (COPD), and carcinogenesis, in animal models. Stereology is considered as the gold standard to obtain quantitative data on lung structure, with a key advantage being to quantify irregular three-dimensional structures on the basis of measurement made on two-dimensional sections...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29321305/arabidopsis-rna-polymerase-v-mediates-enhanced-compaction-and-silencing-of-geminivirus-and-transposon-chromatin-during-host-recovery-from-infection
#19
Tami Coursey, Elizabeth Regedanz, David M Bisaro
Plants employ RNA-directed DNA methylation (RdDM) and dimethylation of histone 3 lysine 9 (H3K9me2) to silence geminiviruses and transposable elements (TEs). We previously showed that canonical RdDM (Pol IV-RdDM) involving RNA polymerases IV and V (Pol IV and Pol V) is required for Arabidopsis thaliana to recover from infection with Beet curly top virus lacking a suppressor protein that inhibits methylation (BCTV L2-). Recovery, which is characterized by reduced viral DNA levels and symptom remission, allows normal floral development...
January 10, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29320926/brain-perfusion-fixation-in-male-pigs-using-a-safer-closed-system
#20
Gracia U Musigazi, Stéphanie De Vleeschauwer, Raf Sciot, Eric Verbeken, Bart Depreitere
Tissue fixation methods are well established for rodents, but not for large animals. We present a simple technique for in situ brain perfusion fixation in a male porcine model, using cervical vessels for inflow and outflow and achieving a closed system. Thirty-four pigs, aged 4.7 ± 0.6 months and weighing 60.7 ± 10.9 kg, were anaesthetised and mechanically ventilated. The ipsilateral common carotid artery and external jugular vein were dissected and constituted the inflow and outflow access, respectively...
January 1, 2018: Laboratory Animals
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