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https://www.readbyqxmd.com/read/28210822/plasma-dna-methylation-of-p16-and-shp1-in-patients-with-b-cell-non-hodgkin-lymphoma
#1
Kai Ding, Xiaoshuang Chen, Yihao Wang, Hui Liu, Wenjing Song, Lijuan Li, Guojin Wang, Jia Song, Zonghong Shao, Rong Fu
BACKGROUND: Early diagnosis and treatment of non-Hodgkin lymphoma (NHL) are progressively important. It has been shown that aberrant promoter methylation contributes to the development and progression of lymphoma. We tried to explore the effect of methylation of p16 and shp1 genes in plasma in the diagnosis of B-NHL patients. METHODS: The methylation of p16 and shp1 genes in plasma were detected by methylation specific polymerase chain reaction in 103 patients with B-NHL, and compared with peripheral blood leukocytes (PBLs) and formaldehyde-fixed paraffin-embedded (FFPE) tumor tissues...
February 16, 2017: International Journal of Clinical Oncology
https://www.readbyqxmd.com/read/28205094/knockdown-of-cardiac-kir3-1-gene-with-sirna-can-improve-bradycardia-in-an-experimental-sinus-bradycardia-rat-model
#2
Yang Li, Xiaodan Fu, Zhi Zhang, Bo Yu
The objective of this study was to explore whether the inhibition of potassium inwardly rectifying channel (Kir3.1) with short interfering RNA (siRNA) can improve bradycardia in an experimental sinus bradycardia rat model. 54 Sprague Dawley (SD) rats were randomly divided into three groups: experimental group, control group, and sham group. Sinus bradycardia model was established in SD rats through chemical ablation of sinoatrial (SA) node with 20% formaldehyde. Variations of Kir3.1 expression at mRNA and protein level were examined with qPCR and Western blotting...
February 15, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28199945/contribution-of-methane-sulfonic-acid-to-new-particle-formation-in-the-atmosphere
#3
Hailiang Zhao, Xiaotong Jiang, Lin Du
Methane sulfonic acid (MSA) is present in substantial concentrations in the gas phase over oceans and coastal regions. We present an investigation into the contribution of MSA to new particle formation with the common atmospheric aerosol nucleation precursors including MSA, methanol, formic acid, acetone, dimethylether, formaldehyde, methyl formate, by making use a quantum chemical approach. Density functional theory calculations indicate that these bimolecular complexes are characterized by the presence of strong inter-molecular hydrogen bonds (SOH⋯O) with large binding energies and thermodynamic equilibrium constants...
February 7, 2017: Chemosphere
https://www.readbyqxmd.com/read/28188396/how-to-obtain-good-morphology-and-antigen-detection-in-the-same-tissue-section
#4
Daša Zupančič, Marjeta Terčelj, Bojan Štrus, Peter Veranič
Most human and animal biopsy samples are routinely embedded in paraffin since this enables the pathologist or researcher to obtain excellent morphology and simplifies storage. Nevertheless, in many cases, the antigen of interest cannot be detected in paraffin section. The alternative available for good immunohistochemistry is preparation of cryosections, which usually provide decent antigen preservation and are frequently used for immunofluorescence. However, cryosections often do not provide efficient morphological details of tissues and cells for pathologic evaluation...
February 10, 2017: Protoplasma
https://www.readbyqxmd.com/read/28186208/preclinical-evaluation-of-bacterially-produced-rsv-g-protein-vaccine-strong-protection-against-rsv-challenge-in-cotton-rat-model
#5
Sandra Fuentes, Laura Klenow, Hana Golding, Surender Khurana
In current study, we evaluated the safety and protective efficacy of recombinant unglycosylated RSV G protein ectodomain produced in E. coli (in presence and absence of oil-in-water adjuvant) in a preclinical RSV susceptible cotton rat challenge model compared to formaldehyde inactivated RSV (FI-RSV) and live RSV experimental infection. The adjuvanted G protein vaccine induced robust neutralization antibody responses comparable to those generated by live RSV infection. Importantly, adjuvanted G protein significantly reduced viral loads in both the lungs and nose at early time points following viral challenge...
February 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28183150/effects-of-dietary-supplementation-of-acanthopanax-senticosus-on-gastrointestinal-tract-development-in-calves
#6
Zhiwei Kong, Hongdong Jie, Chuanshe Zhou, Lianyu Yang, Zhiliang Tan, Wenyan Yang
Twenty-four newborn Holstein dairy male calves (with initial body weight of 38 ± 3.0 kg) were used in a randomized block design experiment to determine effects of dietary supplementation of Acanthopanax senticosus (AS) on gastrointestinal tract development. Calves were fed milk (10%/body weight) three times at 06.00, 12.00 and 18.00 hours daily with one to four treatments during the experimental periods (4 to 28 days): no supplementation of AS (control group, CG); 1.0 g/L•time of micro-powder AS (MP); 1...
February 9, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28183025/melamine-and-or-formaldehyde-exposures-affect-steroidogenesis-via-alteration-of-star-protein-and-testosterone-synthetic-enzyme-expression-in-male-mice
#7
Samah R Khalil, Ashraf Awad, Sozan A Ali
The reproductive effects of melamine and formaldehyde, either alone or in combination, on mature male Swiss mice were investigated. The animals were orally administered melamine (50mg/kg/day), formaldehyde (25mg/kg/day), a mixture of melamine and formaldehyde, or a vehicle control for 65 consecutive days. As a result, the deterioration of sperm characteristics and inhibition of testicular enzyme activity were observed in the melamine- and formaldehyde-exposed groups. In addition, testosterone and luteinizing hormone levels were significantly reduced in the melamine but not in the formaldehyde-exposed group, which correlated with down-regulation of transcription levels of steroidogenic-related genes...
February 6, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/28183013/base-driven-sunlight-oxidation-of-silver-nanoprisms-for-label-free-visual-colorimetric-detection-of-hexahydro-1-3-5-trinitro-1-3-5-triazine-explosive
#8
Yi He, Li Wang
Here we report a label-free method for visual colorimetric detection of hexahydro-1,3,5-trinitro-1,3,5-triazine (HTT) explosive based on base-driven sunlight oxidation of silver nanoprisms (AgNPRs). Under natural sunlight illumination, the surface plasmon of AgNPRs is excited, which populates O2 antibonding orbitals to generate negative-ion state (O2(-)). The resultant O2(-) with a strong oxidation activity can etch AgNPRs to smaller nanodisks with the aid of NaOH aqueous solution, leading to a blue shift of the absorption peak and color change from blue to pink...
January 25, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28181601/ag-lafeo3-fibers-spheres-and-cages-for-ultrasensitive-detection-of-formaldehyde-at-low-operating-temperatures
#9
Yumin Zhang, Jin Zhang, Jianhong Zhao, Zhongqi Zhu, Qingju Liu
Metal oxide semiconductors with special structures and morphologies have attracted considerable attention because of their promising applications in gas sensors. In this paper, Ag-LaFeO3 fibers, spheres and cages have been prepared. Based on X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray spectrometry (EDS), Brunauer-Emmett-Teller (BET) nitrogen adsorption and transmission electron microscopy (TEM) observations, the structure and morphology of the products were characterized...
February 9, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28169473/fluorescence-detection-of-microcapsule-type-self-healing-based-on-aggregation-induced-emission
#10
Young Kyu Song, Beomjin Kim, Tae Hee Lee, Jin Chul Kim, Joon Hyun Nam, Seung Man Noh, Young Il Park
An extrinsic self-healing coating system containing tetraphenylethylene (TPE) in microcapsules was monitored by measuring aggregation-induced emission (AIE). The core healing agent comprised of methacryloxypropyl-terminated polydimethylsiloxane, styrene, benzoin isobutyl ether, and TPE was encapsulated in a urea-formaldehyde shell. The photoluminescence of the healing agent in the microcapsules was measured that the blue emission intensity dramatically increased and the storage modulus also increased up to 10(5) Pa after the photocuring...
February 7, 2017: Macromolecular Rapid Communications
https://www.readbyqxmd.com/read/28168649/combined-orcein-and-martius-scarlet-blue-omsb-staining-for-qualitative-and-quantitative-analyses-of-atherosclerotic-plaques-in-brachiocephalic-arteries-in-apoe-ldlr-mice
#11
Mariusz Gajda, Agnieszka Jasztal, Tomasz Banasik, Ewa Jasek-Gajda, Stefan Chlopicki
Numerous cellular and extracellular components should be analyzed in sections of atherosclerotic plaques to assess atherosclerosis progression and vulnerability. Here, we combined orcein (O) staining for elastic fibers and martius scarlet blue (MSB) polychrome to visualize various morphological contents of plaque in brachiocephalic arteries (BCA) of apoE/LDLR(-/-) mice. Elastic fibers (including broken elastic laminae and 'buried' fibrous caps) were stained purple and they could be easily distinguished from collagen fibers (blue)...
February 6, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28168422/contact-allergy-to-preservatives-in-patients-with-occupational-contact-dermatitis-and-exposure-analysis-of-preservatives-in-registered-chemical-products-for-occupational-use
#12
Jakob Ferløv Schwensen, Ulrik Fischer Friis, Torkil Menné, Mari-Ann Flyvholm, Jeanne Duus Johansen
PURPOSE: The aim of the study is to investigate risk factors for sensitization to preservatives and to examine to which extent different preservatives are registered in chemical products for occupational use in Denmark. METHODS: A retrospective epidemiological observational analysis of data from a university hospital was conducted. All patients had occupational contact dermatitis and were consecutively patch tested with 11 preservatives from the European baseline series and extended patch test series during a 5-year period: 2009-2013...
February 6, 2017: International Archives of Occupational and Environmental Health
https://www.readbyqxmd.com/read/28166624/self-assembly-directed-aerogel-and-membrane-formation-from-a-magnetic-composite-an-approach-to-develop-multifunctional-materials
#13
Balachandran Vivek, Edamana Prasad
Herein, we report the preparation of an aerogel and a membrane from a magnetic composite material by tuning the self-assembly at the molecular level. The gel exhibits excellent oil absorption property and the membrane shows remarkable autonomous self-healing property. The composite is formed from organosilicon modified poly(amido amine) {PAMAM} dendrimer, which are linked with iron oxide nanoparticles and poly vinyl alcohol (PVA). Upon addition of a cross-linker (formaldehyde), the system undergoes a fast self-assembly and gelation process...
February 6, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28159592/the-hematopoiesis-in-gill-and-its-role-in-the-immune-response-of-pacific-oyster-crassostrea-gigas-against-secondary-challenge-with-vibrio-splendidus
#14
Yiqun Li, Xiaorui Song, Weilin Wang, Lingling Wang, Qilin Yi, Shuai Jiang, Zhihao Jia, Xinyu Du, Limei Qiu, Linsheng Song
Increasing evidences have demonstrated that the invertebrate gill is a predominant tissue participating in the immune response during pathogen challenge. In the present study, the hematopoiesis and immune activities in gill of Pacific oyster Crassostrea gigas were investigated. Stem-like cells with big nuclei and thin cytoplasm were found in the tubules of gill filaments, where DNA synthesis is active and hemocytes production are exuberant. The oysters primarily stimulated by formaldehyde-killed Vibrio splendidus exhibited stronger immune responses and enhanced cell regeneration in gill when they encountered the secondary challenge of live V...
February 1, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28158219/tissue-specific-features-of-the-x-chromosome-and-nucleolus-spatial-dynamics-in-a-malaria-mosquito-anopheles-atroparvus
#15
Semen M Bondarenko, Gleb N Artemov, Igor V Sharakhov, Vladimir N Stegniy
Spatial organization of chromosome territories is important for maintenance of genomic stability and regulation of gene expression. Recent studies have shown tissue-specific features of chromosome attachments to the nuclear envelope in various organisms including malaria mosquitoes. However, other spatial characteristics of nucleus organization, like volume and shape of chromosome territories, have not been studied in Anopheles. We conducted a thorough analysis of tissue-specific features of the X chromosome and nucleolus volume and shape in follicular epithelium and nurse cells of the Anopheles atroparvus ovaries using a modern open-source software...
2017: PloS One
https://www.readbyqxmd.com/read/28153745/development-verification-of-methods-for-measurement-of-exhaled-breath-and-environmental-e-vapor-product-aerosol
#16
Michael J Oldham, Karl A Wagner, I Gene Gilman, James B Beach, Jianmin Liu, Ali A Rostami, Mohamadi A Sarkar
Concerns have been raised about the potential health effects of potential bystander exposure to exhaled aerosols from e-vapor products (EVPs). An exhaled breath collection system (EBS) was developed and analytical methods were verified for collection and analysis of exhaled breath from users of EVPs. Analytical methods were adapted and verified for collection of environmental air samples during EVP use in an exposure chamber. Analysis of constituents in exhaled breath focused on nicotine, propylene glycol, and glycerin (because these are reported as the major constituents in EVPs) and selected carbonyl compounds (acetaldehyde, acrolein, and formaldehyde)...
January 30, 2017: Regulatory Toxicology and Pharmacology: RTP
https://www.readbyqxmd.com/read/28152336/the-indoor-volatile-organic-compounds-vocs-characteristics-and-source-identification-in-a-new-university-campus-in-tianjin-china
#17
Jian Kang, Junjie Liu, Jingjing Pei
This study investigates the VOCs constituents and concentration levels on a new university campus, where all of the buildings including classrooms and student dormitories were newly built and decorated within one year. Investigated indoor environments, include dormitories, classrooms and the library. About 30 dormitory buildings with different furniture loading ratio were measured. The characteristics of the indoor VOCs species are analysed and possible sources are identified. The VOCs are analysed with GC-MS method...
February 2, 2017: Journal of the Air & Waste Management Association
https://www.readbyqxmd.com/read/28145691/spatial-distribution-of-ozone-formation-in-china-derived-from-emissions-of-speciated-volatile-organic-compounds
#18
Rongrong Wu, Shaodong Xie
Ozone (O3) pollution is becoming increasingly severe in China. In addition, our limited understanding of the relationship between O3 and volatile organic compounds (VOCs), is an obstacle to improving air quality. By developing an improved source-oriented speciated VOC emission inventory in 2013, we estimated the ozone formation potential (OFP) and investigated its characteristics in China. Besides, a comparison was made between our estimates and space-based observations from the ozone monitoring instrument (OMI) on the National Aeronautics and Space Administration (NASA)'s Aura satellite...
February 15, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28145034/development-of-a-simple-and-effective-silver-staining-protocol-for-detection-of-dna-fragments
#19
Wenjie Liu, Ronghua Li, Habtamu Ayalew, Yanshi Xia, Guihua Bai, Guijun Yan, Kadambot H M Siddique, Peiguo Guo
Silver staining is one of the widely used methods for DNA fragment detection in biological research. Silver staining protocols have been steadily optimized to improve detection efficiency. This research reports a continuous effort to simplify the existing silver staining protocols, lower experiment cost, and improve DNA detection sensitivity and image clarity. The new method only requires three reagents (silver nitrate, sodium hydroxide and formaldehyde) and 6-7 minutes with high detection sensitivity to visualize as low as 14...
January 31, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28139014/encapsulation-and-dynamic-behavior-of-methanol-and-formaldehyde-inside-open-cage-c60-derivatives
#20
Tsukasa Futagoishi, Michihisa Murata, Atsushi Wakamiya, Yasujiro Murata
Methanol (CH3 OH) and formaldehyde (H2 CO) molecules were inserted into an open-cage C60 derivative with a large opening, under high-pressure and high-temperature conditions in solution. Isolation of their molecular complexes in pure form was achieved by the use of recycling HPLC with Buckyprep columns. (1) H NMR spectroscopy, single-crystal X-ray diffraction studies, and DFT calculations revealed the orientation of the encapsulated CH3 OH and H2 CO, both in solution and in the solid state, and the results show that the CH3 group of the CH3 OH and the carbonyl group of the H2 CO point to the bottom of the cages...
January 31, 2017: Angewandte Chemie
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