keyword
MENU ▼
Read by QxMD icon Read
search

NBD

keyword
https://www.readbyqxmd.com/read/28511912/inhibition-of-kdm4a-activity-as-a-strategy-to-suppress-interleukin-6-production-and-attenuate-colitis-induction
#1
Kazuhiro Ishiguro, Osamu Watanabe, Masanao Nakamura, Takeshi Yamamura, Masanobu Matsushita, Hidemi Goto, Yoshiki Hirooka
4-Chloro-7-nitro-2,1,3-benzoxadiazole (NBD-Cl) functions as a hapten and fluoresces upon binding to proteins. Therefore, fluorescence visualization of hapten-proteins is a feature of the colitis induced by NBD-Cl. Using this colitis model, we located activated fibroblasts in the vicinity of hapten-proteins upon colitis induction and observed interleukin (IL)-6 production in the activated fibroblasts. We screened herbal ingredients using primary fibroblasts stimulated with tumor necrosis factor α (TNF-α) and found the suppressive action of Atractylodin on IL-6 production...
May 13, 2017: Clinical Immunology: the Official Journal of the Clinical Immunology Society
https://www.readbyqxmd.com/read/28508550/photoluminescent-peptide-based-nanostructures-as-fret-donor-for-fluorophore-dye
#2
Antonella Accardo, Giancarlo Morelli, Carlo Diaferia, Luigi Vitagliano, Cinzia Giannini, Teresa Sibillano, Valentina Roviello
A great interest has been recently generated by the discovery that peptide-based nanostructures (NSs) endowed with cross-β structure may show interesting photoluminescent (PL) properties. In this scenario, we have shown that NSs formed by PEGylated hexaphenylalanine (PEG8-F6) are able to emit at 460 nm when excited at 370 or at 410 nm. Here, we explored the possibility to transfer the fluorescence of these PEG8-F6-based NSs by Foster Resonance Electron Transfer (FRET) phenomenon to a fluorescent dye. To achieve this aim, we encapsulated in these NSs the 4-Chloro-7-nitrobenzofurazan (NBD) dye...
May 16, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28505454/perturbation-response-scanning-reveals-key-residues-for-allosteric-control-in-hsp70
#3
David Penkler, Ozge Sensoy, Canan Atilgan, Ozlem Tastan Bishop
Hsp70 molecular chaperones play an important role in maintaining cellular homeostasis, and are implicated in a wide array of cellular processes including protein recovery from aggregates, cross membrane protein translocation, and protein biogenesis. Hsp70 consists of two domains, a nucleotide binding domain (NBD) and a substrate binding domain (SBD), each of which communicates via an allosteric mechanism such that the protein interconverts between two functional states, an ATP bound open conformation and an ADP bound closed conformation...
May 15, 2017: Journal of Chemical Information and Modeling
https://www.readbyqxmd.com/read/28503061/use-of-4-fr-versus-6-fr-nasobiliary-catheter-for-biliary-drainage-a-prospective-multicenter-randomized-controlled-study
#4
Tomofumi Tsuboi, Masahiro Serikawa, Tamito Sasaki, Yasutaka Ishii, Yoshifumi Fujimoto, Atsushi Yamaguchi, Takashi Ishigaki, Akinori Shimizu, Keisuke Kurihara, Yumiko Tatsukawa, Eisuke Miyaki, Kazuaki Chayama
Background and Aim. Endoscopic nasobiliary drainage (NBD) effects according to diameter remain unclear. We aimed to assess the drainage effects of the 4-Fr and 6-Fr NBD catheters. Methods. This prospective, multicenter, randomized, controlled study was conducted at Hiroshima University Hospital and related facilities within Hiroshima Prefecture. Endoscopic retrograde cholangiopancreatography (ERCP) in 246 patients revealed acute cholangitis, obstructive jaundice, and/or extrahepatic cholestasis; 4-Fr or 6-Fr NBD catheters were randomly allocated and placed in these patients...
2017: Diagnostic and Therapeutic Endoscopy
https://www.readbyqxmd.com/read/28494384/attentional-blink-to-alcohol-cues-in-binge-drinkers-versus-non-binge-drinkers
#5
Francesco M DePalma, Natalie Ceballos, Reiko Graham
Previous studies have shown alcohol-related attentional biases in social drinkers; however, the temporal dynamics of these biases are not well understood. The current study examined this issue in 94 participants (27 male) categorized as binge drinkers (BD) or non-binge drinkers (NBD). Two versions of an alcohol-related attentional blink (AB) paradigm were used: one with words and one with images. It was predicted that BDs (versus NBDs) would exhibit reduced AB for alcohol cues, which would be enhanced for the pictorial version of the task (versus words)...
April 29, 2017: Addictive Behaviors
https://www.readbyqxmd.com/read/28483280/stacking-cyclodextrin-microchip-electrokinetic-chromatographic-determination-of-gabapentinoid-drugs-in-pharmaceutical-and-biological-matrices
#6
Abdallah M Zeid, Noritada Kaji, Jenny Jeehan M Nasr, Fathalla F Belal, Yoshinobu Baba, Mohamed I Walash
A facile, rapid, and highly sensitive microchip-based electrokinetic chromatographic method was developed for the simultaneous analysis of two gabapentinoid drugs, gabapentin (GPN) and pregabalin (PGN). Both drugs were first reacted with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) via nucleophilic substitution reactions to yield highly fluorescent products with λex/em 470/540nm. Analyses of both fluorescently labeled compounds were achieved within 200s in a poly(methyl methacrylate) (PMMA) microchip with a 30mm separation channel...
April 26, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28467281/chronic-refractory-constipation-due-to-neurogenic-bowel-dysfunction-can-be-successfully-treated-by-sacral-neurostimulation
#7
Frederiek Nuytens, Mathieu D'Hondt
INTRODUCTION: In the last few decades, sacral neurostimulation (SNS) has proven to be an effective treatment option for functional bowel disorders. Experience concerning the role of SNS in the treatment of chronic constipation due to neurogenic bowel dysfunction (NBD) however is limited. METHODS: In this report, we present the case of a 44-year old patient, with chronic refractory neurogenic constipation after a spontaneous cerebral hemorrhage, who was treated with SNS...
May 3, 2017: Acta Chirurgica Belgica
https://www.readbyqxmd.com/read/28456950/a-rapid-fluorescence-assay-for-measuring-sphingosine-1-phosphate-transporter-activity-in-erythrocytes
#8
Naoki Kobayashi, Tsuyoshi Nishi
Sphingosine-1-phosphate (S1P) is an intercellular signaling molecule that is present in the plasma and plays an important role in recruiting lymphocytes from the thymus and secondary lymphoid organs. Erythrocytes are the most abundant cells in the blood and substantially contribute to the S1P supply in the plasma by releasing intracellularly synthesized S1P via an S1P transporter. Thus, the S1P transporter in erythrocytes is a potential target for immuno-suppressing drugs.In this chapter, we describe a rapid method for measuring the activity of the erythrocyte S1P transporter by using the fluorescent S1P analog, 7-nitro-2-1,3-benzoxadiazol-4-yl (NBD)-labeled S1P...
April 30, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28422165/structural-basis-of-inhibition-of-lipid-linked-oligosaccharide-flippase-pglk-by-a-conformational-nanobody
#9
Camilo Perez, Martin Köhler, Daniel Janser, Els Pardon, Jan Steyaert, Renato Zenobi, Kaspar P Locher
PglK is an ABC transporter that flips a lipid-linked oligosaccharide (LLO) that serves as a donor in protein N-glycosylation. Previous structures revealed two inward-facing conformations, both with very large separations of the nucleotide binding domains (NBDs), and a closed, ADP-bound state that featured an occluded cavity. To investigate additional states, we developed conformation-sensitive, single-domain camelid nanobodies (Nb) and studied their effect on PglK activity. Biochemical, structural, and mass spectrometric analyses revealed that one inhibitory Nb binds as a single copy to homodimeric PglK...
April 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28418896/nfkb-is-essential-for-activin-induced-colorectal-cancer-migration-via-upregulation-of-pi3k-mdm2-pathway
#10
Arundhati Jana, Nancy L Krett, Grace Guzman, Ahmer Khalid, Ozkan Ozden, Jonas J Staudacher, Jessica Bauer, Seung Hyun Baik, Timothy Carroll, Cemal Yazici, Barbara Jung
Colorectal cancer (CRC) remains a common and deadly cancer due to metastatic disease. Activin and TGFB (TGFβ) signaling are growth suppressive pathways that exert non-canonical pro-metastatic effects late in CRC carcinogenesis. We have recently shown that activin downregulates p21 via ubiquitination and degradation associated with enhanced cellular migration independent of SMADs. To investigate the mechanism of metastatic activin signaling, we examined activated NFkB signaling and activin ligand expression in CRC patient samples and found a strong correlation...
March 18, 2017: Oncotarget
https://www.readbyqxmd.com/read/28417533/split-tasks-of-asymmetric-nucleotide-binding-sites-in-the-heterodimeric-abc-exporter-efrcd
#11
Lea M Hürlimann, Michael Hohl, Markus A Seeger
Many heterodimeric ATP-binding cassette (ABC) exporters evolved asymmetric ATP-binding sites containing a degenerate site incapable of ATP hydrolysis due to noncanonical substitutions in conserved sequence motifs. Recent studies revealed that nucleotide binding to the degenerate site stabilizes contacts between the nucleotide-binding domains (NBDs) of the inward-facing transporter and regulates ATP hydrolysis at the consensus site via allosteric coupling mediated by the D-loops. However, it is unclear whether nucleotide binding to the degenerate site is strictly required for substrate transport...
April 18, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28399365/tin-ii-hydrides-as-intermediates-in-rearrangements-of-tin-ii-alkyl-derivatives
#12
Shuai Wang, Madison L McCrea-Hendrick, Cory M Weinstein, Christine A Caputo, Elke Hoppe, James C Fettinger, Marilyn M Olmstead, Philip P Power
Reactions of the Sn(II) hydrides [ArSn(μ-H)]2 (1) (Ar = Ar(iPr4) (1a), Ar(iPr6) (1b); Ar(iP4) = C6H3-2,6-(C6H3-2,6-(i)Pr2)2, Ar(iPr6) = C6H3-2,6-(C6H2-2,4,6-(i)Pr3)2) with norbornene (NB) or norbornadiene (NBD) readily generate the bicyclic alkyl-/alkenyl-substituted stannylenes, ArSn(norbornyl) (2a or 2b) and ArSn(norbornenyl) (3a or 3b), respectively. Heating a toluene solution of 3a or 3b at reflux afforded the rearranged species ArSn(3-tricyclo[2.2.1.0(2,6)]heptane) (4a or 4b), in which the norbornenyl ligand is transformed into a nortricyclyl group...
May 5, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28383632/measuring-change-in-symptoms-of-neurobehavioural-disability-responsiveness-of-the-st-andrew-s-swansea-neurobehavioural-outcome-scale
#13
Nick Alderman, Claire Williams, Caroline Knight, Rodger Ll Wood
Objective: Neurobehavioural disability (NBD) after acquired brain injury (ABI) is often associated with poor outcome. The "St Andrew's-Swansea Neurobehavioural Outcome Scale" (SASNOS) was developed to measure NBD in a range of applications. Two of the "holy trinity" of psychometric properties, reliability and validity, have been comprehensively mapped, but the extent to which SASNOS meets the third, responsiveness, has not been investigated. Demonstrating responsiveness is essential in instruments employed in repeated measurement scenarios to confirm their ability to discriminate real change from error...
April 5, 2017: Archives of Clinical Neuropsychology: the Official Journal of the National Academy of Neuropsychologists
https://www.readbyqxmd.com/read/28358164/thiolysis-of-nbd-based-dyes-for-colorimetric-and-fluorescence-detection-of-h2s-and-biothiols-design-and-biological-applications
#14
REVIEW
Long Yi, Zhen Xi
Hydrogen sulfide (H2S) is an important endogenous signaling molecule with multiple biological functions. The development of colorimetric and fluorescent probes for the visualization of H2S is useful for studying H2S biology. This review details up-to-date research on H2S probes based on thiolysis reactions of NBD (7-nitro-1,2,3-benzoxadiazole) amines or ethers (thioethers). The reactivity and selectivity of NBD-based dyes are discussed. The design principles of NBD-based probes are highlighted. The advantages and disadvantages of NBD-based probes are compared, and their biological applications are discussed...
May 10, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28358005/structural-intermediates-and-directionality-of-the-swiveling-motion-of-pyruvate-phosphate-dikinase
#15
Alexander Minges, Daniel Ciupka, Christian Winkler, Astrid Höppner, Holger Gohlke, Georg Groth
Pyruvate phosphate dikinase (PPDK) is a vital enzyme in cellular energy metabolism catalyzing the ATP- and Pi-dependent formation of phosphoenolpyruvate from pyruvate in C4 -plants, but the reverse reaction forming ATP in bacteria and protozoa. The multi-domain enzyme is considered an efficient molecular machine that performs one of the largest single domain movements in proteins. However, a comprehensive understanding of the proposed swiveling domain motion has been limited by not knowing structural intermediates or molecular dynamics of the catalytic process...
March 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28356133/abdominal-massage-for-neurogenic-bowel-dysfunction-in-people-with-multiple-sclerosis-amber-abdominal-massage-for-bowel-dysfunction-effectiveness-research-study-protocol-for-a-randomised-controlled-trial
#16
Doreen McClurg, Kirsteen Goodman, Suzanne Hagen, Fional Harris, Sean Treweek, Anton Emmanuel, Christine Norton, Maureen Coggrave, Selina Doran, John Norrie, Peter Donnan, Helen Mason, Sarkis Manoukian
BACKGROUND: Multiple sclerosis (MS) is a life-long condition primarily affecting younger adults. Neurogenic bowel dysfunction (NBD) occurs in 50-80% of these patients and is the term used to describe constipation and faecal incontinence, which often co-exist. Data from a pilot study suggested feasibility of using abdominal massage for the relief of constipation, but the effectiveness remains uncertain. METHODS/DESIGN: This is a multi-centred patient randomised superiority trial comparing an experimental strategy of once daily abdominal massage for 6 weeks against a control strategy of no massage in people with MS who have stated that their constipation is bothersome...
March 29, 2017: Trials
https://www.readbyqxmd.com/read/28315324/nucleolin-protects-macrophages-from-oxldl-induced-foam-cell-formation-through-up-regulating-abca1-expression
#17
Yuanbin Li, Bimei Jiang, Pengfei Liang, Zhongyi Tong, Meidong Liu, Qinglan Lv, Yanjuan Liu, Xuanyou Liu, Yuting Tang, Xianzhong Xiao
Our recent studies have indicated that nucleolin, as a multifunctional RNA-binding protein, exerts protective effects in the myocardial cells and endothelial cells under the condition of oxidative stress. However, the function of nucleolin and its potential mechanism in macrophage-derived foam cell formation remain largely unexplored. ApoE-/- mice were fed with a high-fat diet (HFD) for 10-24 weeks. Protein expression was measured by western blotting or immunofluorescence, and gene expression at the mRNA level was detected by qRT-PCR...
April 29, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28306394/measuring-discourse-coherence-in-anomic-aphasia-using-rhetorical-structure-theory
#18
Anthony Pak-Hin Kong, Anastasia Linnik, Sam-Po Law, Waisa Wai-Man Shum
PURPOSE: The existing body of work regarding discourse coherence in aphasia has provided mixed results, leaving the question of coherence being impaired or intact as a result of brain injury unanswered. In this study, discourse coherence in non-brain-damaged (NBD) speakers and speakers with anomic aphasia was investigated quantitatively and qualitatively. METHOD: Fifteen native speakers of Cantonese with anomic aphasia and 15 NBD participants produced 60 language samples...
March 17, 2017: International Journal of Speech-language Pathology
https://www.readbyqxmd.com/read/28293837/treatment-of-plants-with-gaseous-ethylene-and-gaseous-inhibitors-of-ethylene-action
#19
Mark L Tucker, Joonyup Kim, Chi-Kuang Wen
The gaseous nature of ethylene affects not only its role in plant biology but also how you treat plants with the hormone. In many ways, it simplifies the treatment problem. Other hormones have to be made up in solution and applied to some part of the plant hoping the hormone will be taken up into the plant and translocated throughout the plant at the desired concentration. Because all plant cells are connected by an intercellular gas space the ethylene concentration you treat with is relatively quickly reached throughout the plant...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28286085/hspa5-gene-encoding-hsp70-chaperone-bip-in-the-endoplasmic-reticulum
#20
REVIEW
Jie Wang, Jessica Lee, David Liem, Peipei Ping
The HSPA5 gene encodes the binding immunoglobulin protein (BiP), an Hsp70 family chaperone localized in the ER lumen. As a highly conserved molecular chaperone, BiP assists in a wide range of folding processes via its two structural domains, a nucleotide-binding domain (NBD) and substrate-binding domain (SBD). BiP is also an essential component of the translocation machinery for protein import into the ER, a regulator for Ca(2+) homeostasis in the ER, as well as a facilitator of ER-associated protein degradation (ERAD) via retrograde transportation of aberrant proteins across the ER membrane...
June 30, 2017: Gene
keyword
keyword
50687
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"