keyword
MENU ▼
Read by QxMD icon Read
search

mg132

keyword
https://www.readbyqxmd.com/read/29678153/radiosensitization-of-clioquinol-and-zinc-in-human-cancer-cell-lines
#1
Shan Lu, Yuan Ke, Chaoyan Wu, Yahua Zhong, Conghua Xie, Yunfeng Zhou, Fuxiang Zhou, Haijun Yu
BACKGROUND: We previously reported that clioquinol acts as a zinc ionophore and inhibits the NF-κB signalling pathway. Other research has demonstrated that zinc deficiency plays a vital role in the occurrence and development of some solid tumours, and intracellular zinc supplementation may reverse this process and enhance the tumour sensitivity to anticancer treatment. Thus, we investigated the radiosensitization effects of clioquinol combined with zinc on HeLa and MCF-7 cells in vitro...
April 20, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29669812/tankyrase-modulates-insulin-sensitivity-in-skeletal-muscle-cells-by-regulating-the-stability-of-glut4-vesicle-proteins
#2
Zhiduan Su, Vinita Deshpande, David E James, Jacqueline Stöckli
Tankyrase1 and 2, members of the poly(ADP-ribose) polymerase family, have previously been shown to play a role in insulin-mediated glucose uptake in adipocytes. However, their precise mechanism of action, and their role in insulin action in other cell types, such as myocytes, remains elusive. Treatment of differentiated L6 myotubes with the small molecule tankyrase inhibitor XAV939 resulted in insulin resistance as determined by impaired insulin-stimulated glucose uptake. Proteomic analysis of XAV939-treated myotubes identified down-regulation of several glucose transporter GLUT4 storage vesicle (GSV) proteins including RAB10, VAMP8, SORT1 and GLUT4...
April 18, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29669333/the-role-of-proteasome-inhibitor-mg132-in-2-4-dinitrofluorobenzene-induced-atopic-dermatitis-in-nc-nga-mice
#3
Kozo Ohkusu-Tsukada, Daiki Ito, Kimimasa Takahashi
BACKGROUND: Although immunosuppressants for therapy of atopic dermatitis (AD) are still being sought, proteasome inhibitors are also potential candidates for the treatment of AD. Proteasome inhibitors exert various effects by blocking proteasomal degradation and help regulate processes such as apoptosis induction via caspase-9, cell cycle progression via cyclins, NF-κB inactivation via IκB, and downregulation of antigen cross-presentation. The cells targeted by proteasome inhibitors are therefore activated cells undergoing proliferation or differentiation, and antigen-presenting cells carrying out protein degradation...
April 18, 2018: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/29668859/kribb53-binds-to-oct4-and-enhances-its-degradation-through-the-proteasome-causing-apoptotic-cell-death-of-oct4-positive-testicular-germ-cell-tumors
#4
Jiyae Jung, Youngmi Kim, Jinhoi Song, Yae Jin Yoon, Da-Eun Kim, Joo Ae Kim, Yena Jin, Yu-Jin Lee, Seokho Kim, Byoung-Mog Kwon, Dong Cho Han
We hypothesized that Octamer-binding transcription factor 4 (OCT4) inhibition would have therapeutic benefits in Testicular germ cell tumors (TGCT). To identify inhibitors of OCT4, a chemical library was screened using a luciferase reporter system under the control of an OCT4 response element. A compound named KRIBB53 was identified based on its blocking of OCT4-dependent luciferase activation. When NCCIT cells were exposed to KRIBB53, the expression levels of OCT4 target genes, such as NANOG and USP44, were inhibited with an IC50 of 13 and 15 μM, respectively...
April 13, 2018: Carcinogenesis
https://www.readbyqxmd.com/read/29665476/bioactive-norditerpenoids-from-cephalotaxus-fortunei-var-alpina-and-c-lanceolata
#5
Ling Ni, Xiu-Hong Zhong, Xiao-Jiao Chen, Bing-Jie Zhang, Mei-Fen Bao, Xiang-Hai Cai
Twenty-eight naturally occurring Cephalotaxus tropone analogues, including 19 previously undescribed ones, were identified from Cephalotaxus fortunei Hook. var. alpina H. L. Li and C. lanceolata K. M. Feng. The presence of the C20 cephinoids A-E revealed that these tropones were assigned to the norditerpenoids and were perhaps derived from labdane-type diterpenoids. These norditerpenoids showed excellent cytotoxicity against human cancer cells (IC50 , 20-0.1 μM) in vitro. The SAR (structure-activity relationship) analysis disclosed that the tropone moiety and the lactone ring were crucial structural features for the observed activities...
April 14, 2018: Phytochemistry
https://www.readbyqxmd.com/read/29655799/stage-specific-expression-of-the-proline-alanine-transporter-in-the-human-pathogen-leishmania
#6
T Liburkin-Dan, D Schlisselberg, R Fischer-Weinberger, P Pescher, E Inbar, M Ephros, D Rentsch, G F Späth, D Zilberstein
Leishmania are obligatory intracellular parasites that cycle between the sand fly midgut (extracellular promastigotes) and mammalian macrophage phagolysosomes (intracellular amastigotes). They have developed mechanisms of adaptation to the distinct environments of host and vector that favor utilization of both proline and alanine. LdAAP24 is the L. donovani proline-alanine transporter. It is a member of Leishmania system A that translocates neutral amino acids. Since system A is promastigote-specific, we aimed to assess whether LdAAP24 is also expressed exclusively in promastigotes...
April 12, 2018: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/29650159/expression-of-clavata3-fusions-indicates-rapid-intracellular-processing-and-a-role-of-erad
#7
Francesca De Marchis, Sara Colanero, Eva M Klein, Davide Mainieri, Viviana M Prota, Michele Bellucci, Giampiero Pagliuca, Elisa Zironi, Teresa Gazzotti, Alessandro Vitale, Andrea Pompa
The 12 amino acid peptide derived from the Arabidopsis soluble secretory protein CLAVATA3 (CLV3) acts at the cell surface in a signalling system that regulates the size of apical meristems. The subcellular pathway involved in releasing the peptide from its precursor is unknown. We show that a CLV3-GFP fusion expressed in transfected tobacco protoplasts or transgenic tobacco plants has very short intracellular half-life that cannot be extended by the secretory traffic inhibitors brefeldin A and wortmannin. The fusion is biologically active, since the incubation medium of protoplasts from CLV3-GFP-expressing tobacco contains the CLV3 peptide and inhibits root growth...
June 2018: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/29645000/neotuberostemonine-inhibits-the-differentiation-of-lung-fibroblasts-into-myofibroblasts-in-mice-by-regulating-hif-1%C3%AE-signaling
#8
Xin-Miao Lv, Ming-Dan Li, Si Cheng, Bao-Lin Liu, Kang Liu, Chao-Feng Zhang, Xiang-Hong Xu, Mian Zhang
Pulmonary fibrosis may be partially the result of deregulated tissue repair in response to chronic hypoxia. In this study we explored the effects of hypoxia on lung fibroblasts and the effects of neotuberostemonine (NTS), a natural alkaloid isolated from Stemona tuberosa, on activation of fibroblasts in vitro and in vivo. PLFs (primary mouse lung fibroblasts) were activated and differentiated after exposure to 1% O2 or treatment with CoCl2 (100 μmol/L), evidenced by markedly increased protein or mRNA expression of HIF-1α, TGF-β, FGF2, α-SMA and Col-1α/3α, which was blocked after silencing HIF-1α, suggesting that the activation of fibroblasts was HIF-1α-dependent...
April 12, 2018: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/29628506/zinc-finger-protein-746-promotes-colorectal-cancer-progression-via-c-myc-stability-mediated-by-glycogen-synthase-kinase-3%C3%AE-and-f-box-and-wd-repeat-domain-containing-7
#9
Ji Hoon Jung, Deok-Beom Jung, Hyunseok Kim, Hyemin Lee, Shi-Eun Kang, Sanjay K Srivastava, Miyong Yun, Sung-Hoon Kim
To elucidate the underlying oncogenic mechanism of zinc finger protein 746 (ZNF746), current study was conducted in colorectal cancers (CRCs). Herein, ZNF746 was overexpressed in HCT116, SW620, and SW480 cells, which was supported by CRC tissue microarray and TCGA analysis. Also, DNA microarray revealed the differentially expressed gene profile particularly related to cell cycle genes and c-Myc in ZNF746 depleted HCT116 cells. Furthermore, ZNF746 enhanced the stability of c-Myc via their direct binding through nuclear colocalization by immunoprecipitation and immunofluorescence, while ZNF746 and c-Myc exist mainly in nucleoplasm...
April 9, 2018: Oncogene
https://www.readbyqxmd.com/read/29625985/importance-of-proteasome-gene-expression-during-model-dough-fermentation-after-freezing-preservation-of-baker-s-yeast-cells
#10
Daisuke Watanabe, Hiroshi Sekiguchi, Yukiko Sugimoto, Atsushi Nagasawa, Naotaka Kida, Hiroshi Takagi
Freeze-thaw stress causes various types of cellular damages, survival and/or proliferation defects, and metabolic alterations. However, the mechanisms underlying how cells cope with freeze-thaw stress are poorly understood. Here, model dough fermentations using two baker's yeast strains, 45 and YF, of Saccharomyces cerevisiae were compared after 2-week cell preservation in a refrigerator or freezer. YF exhibited slow fermentation after exposure to freeze-thaw stress due to the low cell viability. A DNA microarray analysis of the YF cells during fermentation revealed that the genes involved in oxidative phosphorylation were relatively strongly expressed, suggesting a decrease in the glycolytic capacity...
April 6, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29580840/hiv-1-promotes-the-degradation-of-components-of-the-type-1-ifn-jak-stat-pathway-and-blocks-anti-viral-isg-induction
#11
Siobhan Gargan, Suaad Ahmed, Rebecca Mahony, Ciaran Bannan, Silvia Napoletano, Cliona O'Farrelly, Persephone Borrow, Colm Bergin, Nigel J Stevenson
Anti-retroviral therapy successfully suppresses HIV-1 infection, but fails to provide a cure. During infection Type 1 IFNs normally play an essential role in viral clearance, but in vivo IFN-α only has a modest impact on HIV-1 infection, suggesting its possible targeting by HIV. Here, we report that the HIV protein, Vif, inhibits effective IFN-α signalling via degradation of essential JAK/STAT pathway components. We found that STAT1 and STAT3 are specifically reduced in HEK293T cells expressing Vif and that full length, infectious HIV-1 IIIB strain promotes their degradation in a Vif-dependent manner...
March 9, 2018: EBioMedicine
https://www.readbyqxmd.com/read/29576851/autophagy-stimulus-promotes-early-hur-protein-activation-and-p62-sqstm1-protein-synthesis-in-arpe-19-cells-by-triggering-erk1-2-p38-mapk-and-jnk-kinase-pathways
#12
Nicoletta Marchesi, Natthakan Thongon, Alessia Pascale, Alessandro Provenzani, Ali Koskela, Eveliina Korhonen, Adrian Smedowski, Stefano Govoni, Anu Kauppinen, Kai Kaarniranta, Marialaura Amadio
RNA-binding protein dysregulation and altered expression of proteins involved in the autophagy/proteasome pathway play a role in many neurodegenerative disease onset/progression, including age-related macular degeneration (AMD). HuR/ELAVL1 is a master regulator of gene expression in human physiopathology. In ARPE-19 cells exposed to the proteasomal inhibitor MG132, HuR positively affects at posttranscriptional level p62 expression, a stress response gene involved in protein aggregate clearance with a role in AMD...
2018: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29565467/berberine-decelerates-glucose-metabolism-via-suppression-of-mtor%C3%A2-dependent-hif%C3%A2-1%C3%AE-protein-synthesis-in-colon-cancer-cells
#13
Liyuan Mao, Qiongyun Chen, Ke Gong, Xiaolin Xu, Yurou Xie, Wenqing Zhang, Hanwei Cao, Tianhui Hu, Xiaoting Hong, Yan-Yan Zhan
Hyperactivated glucose uptake and glycolytic metabolism are considered as a hallmark of cancer. Berberine, a natural alkaloid with tumor‑selective anticancer effects, has been shown to promote glucose uptake in metabolic tissues and cells. However, whether and how berberine regulates the glucose metabolism of cancer cells are still poorly understood. In the present study, we revealed that berberine, which suppressed the growth of colon cancer cell lines HCT116 and KM12C, greatly inhibited the glucose uptake and the transcription of glucose metabolic genes, GLUT1, LDHA and HK2 in these two cell lines as assessed by RT‑qPCR...
May 2018: Oncology Reports
https://www.readbyqxmd.com/read/29536250/role-of-the-ubiquitin-proteasome-system-on-acth-turnover-in-rat-corticotropes
#14
Antonella Sesta, Maria Francesca Cassarino, Francesco Cavagnini, Francesca Pecori Giraldi
PURPOSE: A large number of studies has investigated proopiomelanocortin processing in anterior pituitary corticotropes but little is known on proopiomelanocortin/ACTH degradation within these cells. The ubiquitin-proteasome system is an intracellular protein degradation pathway which has garnered considerable interest in recent times, given its role in maintenance of protein homeostasis. Aim of the present study was to evaluate the role of the ubiquitin-proteasome system in proopiomelanocortin/ACTH turnover in pituitary corticotropes...
March 13, 2018: Endocrine
https://www.readbyqxmd.com/read/29533961/first-in-class-combination-therapy-of-a-copper-ii-metallo-phosphorus-dendrimer-with-cytotoxic-agents
#15
Serge Mignani, Nabil El Brahmi, Thierry Cresteil, Jean-Pierre Majoral
The metallo-phosphorus dendrimer 1G3-Cu (generation 3 dendrimer bearing 48 conjugated copper(II) on its surface) has antiproliferative activity related to its capacity to activate Bax translocation. In the present study, we evaluate the activity of an association of 1G3-Cu with 5 cytotoxic agents used in chemotherapy having different modes of action. Data show no additive effect with camptothecin and cisplatin, additivity with paclitaxel and MG132, and synergy with doxorubicin. Results suggest that the multivalent Cu-conjugated dendrimer 1G3-Cu (activator of Bax translocation) plays an important role in boosting the clinical impact of Bax accumulation stimulated by the proteasome inhibitor MG132, antimitotic taxanes, and the topo II inhibitor doxorubicin...
March 13, 2018: Oncology
https://www.readbyqxmd.com/read/29531806/quantitative-peptidomics-of-endogenous-peptides-involved-in-tgf-%C3%AE-1-induced-epithelial-mesenchymal-transition-of-renal-epithelial-cells
#16
Rattiyaporn Kanlaya, Visith Thongboonkerd
TGF-β1 is a key fibrotic factor mediating epithelial mesenchymal transition (EMT) of epithelial cells through various signaling pathways. However, roles of proteolytic cleavage and endogenous peptide dynamics in TGF-β1-induced EMT remain unknown. We therefore performed quantitative peptidomics of TGF-β1-induced EMT in renal tubular epithelial cells. The acquired mesenchymal characteristics were confirmed, including morphological change (from cobblestone-like to fibroblast-like), decreased epithelial marker (ZO-1), and increased mesenchymal marker (vimentin)...
December 2018: Cell Death Discovery
https://www.readbyqxmd.com/read/29524646/cigarette-smoke-constituents-cause-endothelial-nitric-oxide-synthase-dysfunction-and-uncoupling-due-to-depletion-of-tetrahydrobiopterin-with-degradation-of-gtp-cyclohydrolase
#17
Tamer M Abdelghany, Raed S Ismail, Fatma A Mansoor, Joseph R Zweier, Frazer Lowe, Jay L Zweier
Cigarette smoking (CS) is a well-established risk factor for cardiovascular disease (CVD). Endothelial dysfunction (ED) with loss of nitric oxide (NO) production is a central mechanism leading to the advent of CVD. Despite many prior studies of this major health problem, the exact mechanism by which CS induces ED is not well understood. This study examines the mechanism by which CS induces ED with altered endothelial NO synthase (eNOS) function in aortic endothelial cells (AECs). Exposure of AECs to cigarette smoke extract (CSE) resulted in a marked decrease in NO production with concomitant increase in superoxide (O2 ...
March 7, 2018: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/29522752/rna-helicase-ddx5-participates-in-oxldl-induced-macrophage-scavenger-receptor-1-expression-by-suppressing-mrna-degradation
#18
Wenting Zhao, Zhen Wang, Zewei Sun, Yuxian He, Dongdong Jian, Xiaotong Hu, Wang Zhang, Liangrong Zheng
The DEAD box protein DDX5, an ATP-dependent RNA helicase, plays an important role in transcriptional regulation and is associated with solid tumors and leukemia. However, its role in oxLDL-induced lipid uptake in macrophages remains unclear. In this study, we detected the expression of DDX5 mRNA and protein in oxidized low-density lipoprotein (oxLDL)-treated human primary macrophages that were induced from monocytes isolated from human peripheral blood with or without several chemical inhibitors using quantitative real-time PCR (qRT-PCR) or Western blotting...
March 6, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29515774/unbiased-compound-protein-interface-mapping-and-prediction-of-chemoresistance-loci-through-forward-genetics-in-haploid-stem-cells
#19
Moritz Horn, Virginia Kroef, Kira Allmeroth, Nicole Schuller, Stephan Miethe, Martin Peifer, Josef M Penninger, Ulrich Elling, Martin S Denzel
Forward genetic screens in haploid mammalian cells have recently emerged as powerful tools for the discovery and investigation of recessive traits. Use of the haploid system provides unique genetic tractability and resolution. Upon positive selection, these screens typically employ analysis of loss-of-function (LOF) alleles and are thus limited to non-essential genes. Many relevant compounds, including anti-cancer therapeutics, however, target essential genes, precluding positive selection of LOF alleles. Here, we asked whether the use of random and saturating chemical mutagenesis might enable screens that identify essential biological targets of toxic compounds...
February 9, 2018: Oncotarget
https://www.readbyqxmd.com/read/29491214/bisphenol-a-and-its-derivatives-induce-degradation-of-hif-1alpha-via-the-lysosomal-pathway-in-human-hepatocarcinoma-cell-line-hep3b
#20
Yukino Kobayashi, Ami Oguro, Susumu Imaoka
Bisphenol A (BPA, 2,2-bis(4-hydroxyphenyl)propane), one of the phenolic compounds widely used in the manufacture of plastic and epoxy resins, is known as an endocrine disruptor. In a previous study, we found that BPA induced hypoxia inducible factor-1alpha (HIF-1alpha) degradation by dissociation from heat shock protein 90 (Hsp90). In this study, to investigate the structural requirements for degradation of HIF-1alpha, we estimated the effect of BPA derivatives (BPE, BPF, BPB, Dimethyl butylidene diphenol (DMBDP), Ethyl hexylidene diphenol (EHDP), Bishydroxyphenyl cyclohexane (BHCH), and Methyl benzylidene bisphenol (MBBP)) on HIF-1alpha protein degradation, using human hepatocarcinoma cell line, Hep3B...
2018: Biological & Pharmaceutical Bulletin
keyword
keyword
50658
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"