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https://www.readbyqxmd.com/read/28485891/%C3%AE-syntrophin-stabilises-catalase-to-reduce-endogenous-reactive-oxygen-species-levels-during-myoblast-differentiation
#1
Jae Yun Moon, Su Jin Choi, Cheol Ho Heo, Hwan Myung Kim, Hye Sun Kim
α-Syntrophin is a component of the dystrophin-glycoprotein complex that interacts with various intracellular signaling proteins in muscle cells. The α-syntrophin knock-down C2 cell line (SNKD), established by infecting lentivirus particles with α-syntrophin shRNA, is characterized by a defect in terminal differentiation and increase in cell death. Since myoblast differentiation is accompanied by intensive mitochondrial biogenesis, the generation of intracellular reactive oxygen species (ROS) is also increased during myogenesis...
May 9, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28484439/amino-acid-residues-68-71-contribute-to-influenza-a-virus-pb1-f2-protein-stability-and-functions
#2
Yi-Ying Cheng, Shih-Rang Yang, Ying-Ting Wang, Yu-Hsin Lin, Chi-Ju Chen
Influenza A virus PB1-F2, encoding a multi-functional protein, is regarded as a virulent gene. Variation in expression pattern and protein stability among PB1-F2 proteins derived from different strains may explain why PB1-F2 functions in a strain- and cell type-specific manner. Because the protein stability of PB1-F2 affects its biological functions, we looked for sequences important for this property. By comparing variants and chimeric of PB1-F2 proteins from A/Hong Kong/156/1997 (H5N1) and A/Puerto Rico/8/1934 (H1N1), we identified amino acid residues 68-71 affect its protein stability...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28465088/nrf2-are-signaling-provides-neuroprotection-in-traumatic-brain-injury-via-modulation-of-the-ubiquitin-proteasome-system
#3
Hui Ding, Xiaoliang Wang, Handong Wang, Lin Zhu, Qiang Wang, Yue Jia, Wuting Wei, Chenhui Zhou, Heming Wu, Ke Ding
The nuclear factor erythroid 2-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway exhibits protective effects in a variety of neurological diseases. However, the role of this pathway in traumatic brain injury (TBI) is not fully understood. This study investigates whether the Nrf2-ARE pathway provides neuroprotection following TBI via regulation of the ubiquitin proteasome system (UPS), and examines the involvement of this pathway in redox homeostasis. We found that activation the Nrf2-ARE pathway can mitigate secondary brain injury induced by TBI...
April 29, 2017: Neurochemistry International
https://www.readbyqxmd.com/read/28446729/wp1130-attenuates-cisplatin-resistance-by-decreasing-p53-expression-in-non-small-cell-lung-carcinomas
#4
Xiang Wang, Ying Bao, Zhaohui Dong, Qiuqiang Chen, Huihui Guo, Charlie Xiang, Jianzhong Shao
Cisplatin-based combination chemotherapy significantly improves the survival outcomes in non-small cell lung carcinomas (NSCLCs), but drug resistance commonly contributes to disease progression and relapse. Recently, accumulating evidence has indicated that deubiquitinases (DUBs) are involved in regulating tumor cell proliferation, apoptosis, and chemoresistance. We designed this study to investigate the role of WP1130, a DUB inhibitor, in regulating cisplatin cytotoxicity in NSCLCs. After being combined with WP1130, cisplatin sensitivity was significantly increased in A549 and HCC827 cells with decreased p53 expression, inhibiting their proliferation, but not in p53-deficient NCI-H1299 cells...
April 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28438434/connective-tissue-growth-factor-decreases-mitochondrial-metabolism-through-ubiquitin-mediated-degradation-of-mitochondrial-transcription-factor-a-in-oral-squamous-cell-carcinoma
#5
Wei-Ting Lai, Yue-Ju Li, Shi-Bei Wu, Cheng-Ning Yang, Tai-Sheng Wu, Yau-Huei Wei, Yi-Ting Deng
BACKGROUND/PURPOSE: Deregulation of metabolic pathways is one of the hallmarks of cancer progression. Connective tissue growth factor (CTGF/CCN2) acts as a tumor suppressor in oral squamous cell carcinoma (OSCC). However, the role of CTGF in modulating cancer metabolism is still unclear. METHODS: OSCC cells stably overexpressing CTGF (SAS/CTGF) and shRNA against CTGF (TW2.6/shCTGF) were established. Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were examined by the Seahorse XF24 analyzer...
April 21, 2017: Journal of the Formosan Medical Association, Taiwan Yi Zhi
https://www.readbyqxmd.com/read/28427998/nitric-oxide-regulated-proteolysis-of-human-cyp2b6-via-the-ubiquitin-proteasome-system
#6
Choon-Myung Lee, Shweta Tripathi, Edward T Morgan
We showed previously that rat cytochrome P450 CYP2B1 undergoes NO-dependent proteasomal degradation in response to inflammatory stimuli, and that the related human enzyme CYP2B6 is also down-regulated by NO in primary human hepatocytes. To investigate the mechanism of CYP2B6 down-regulation, we made several cell lines (HeLa and HuH7 cells) in which native CYP2B6 or CYP2B6 with a C-terminal V5 tag (CYP2B6V5) are expressed from a lentiviral vector with a cytomegalovirus promoter. Native CYP2B6 protein was rapidly down-regulated in HeLa cells within 3h of treatment with the NO donor (Z)-1-[2-(2-Aminoethyl)-N-(2-ammonioethyl)amino]diazen-1-ium-1,2-diolate, while its mRNA level was not down-regulated...
April 17, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28424976/the-involvement-of-nr2b-and-tau-protein-in-mg132-induced-creb-dephosphorylation
#7
Min Xie, Yuan Li, Shao-Hui Wang, Qun-Tao Yu, Xin Meng, Xiao-Mei Liao
Transcription factor cAMP response element-binding protein (CREB) plays a critical role in memory formation. Ubiquitin-proteasome system-dependent protein degradation affects the upstream signaling pathways which regulate CREB activity. However, the molecular mechanisms of proteasome inhibition on reductive CREB activity are still unclear. The current study demonstrated that MG132-inhibited proteasome activity resulted in a dose dependence of CREB dephosphorylation at Ser133 as well as decreased phosphorylation of N-methyl-D-aspartate (NMDA) receptor subunit NR2B (Tyr1472) and its tyrosine protein kinase Fyn (Tyr416)...
April 19, 2017: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/28419994/degradation-of-mcl-1-through-gsk-3%C3%AE-activation-regulates-apoptosis-induced-by-bufalin-in-non-small-cell-lung-cancer-h1975-cells
#8
Xiao-Hong Kang, Jing-Hang Zhang, Qing-Qin Zhang, Yan-Hui Cui, Ying Wang, Wei-Zheng Kou, Zhan-Hui Miao, Ping Lu, Li-Fang Wang, Zhen-Ye Xu, Fei Cao
BACKGROUND/AIMS: Mcl-1, an anti-apoptotic Bcl-2 family member, is often overexpressed in non-small cell lung cancer (NSCLC). Bufalin has been reported to induce apoptosis in various tumor cells. However, there is no report showing that bufalin could downregulate Mcl-1 expression in NSCLC. METHODS: Cell proliferation was analyzed by cell counting kit-8 (CCK-8) assay in H1975 cells. Cell apoptosis was detected by flow cytometry. Mcl-1 mRNA was detected by RT-PCR. The expression of apoptosis-associated proteins in H1975 cells was detected by western blotting...
April 14, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28418925/syntenin-promotes-vegf-induced-vegfr2-endocytosis-and-angiogenesis-by-increasing-ephrin-b2-function-in-endothelial-cells
#9
Nara Tae, Suhyun Lee, Okwha Kim, Juhee Park, Sunghun Na, Jeong-Hyung Lee
Syntenin, a tandem PDZ-domain-containing scaffold protein, is involved in the regulation of diverse biological functions, including protein trafficking, exosome biogenesis, and cancer metastasis. Here, we present the first study to explore the significance of syntenin in endothelial cells. Syntenin knockdown in human umbilical vein endothelial cells (HUVECs) impaired vascular endothelial growth factor (VEGF)-mediated proliferation, migration, invasion, vascular permeability, and nitric oxide (NO) production...
March 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28412401/proteasome-inhibitor-loaded-micelles-enhance-antitumor-activity-through-macrophage-reprograming-by-nf-%C3%AE%C2%BAb-inhibition
#10
Hailiang Wu, Anqi Tao, John D Martin, Sabina Quader, Xueying Liu, Kei Takahashi, Louise Hespel, Yutaka Miura, Yoshihiro Hayakawa, Tatsuro Irimura, Horacio Cabral, Kazunori Kataoka
Macrophage reprogramming towards a tumor attacking phenotype is a promising treatment strategy, yet such strategies are scarce and it is not clear how to combine them with cytotoxic therapies that are often used to treat solid tumors. Here, we evaluate whether a micelle-encapsulated proteasome inhibitor, i.e. the peptide aldehyde drug MG132, which is cytotoxic to cancer cells, can reprogram macrophages to attack the tumor. Through in vitro studies, we demonstrated that the proteasome inhibition reduces nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling - a known promoter of tumor-supporting macrophages and chemoresistance - in both cancer cells and macrophages...
April 12, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28407860/effects-of-mg132-on-the-in%C3%A2-vitro-development-and-epigenetic-modification-of-debao-porcine-somatic-cell-nuclear-transfer-embryos
#11
Kaiyuan Shen, Xiangping Li, Xiaoli Dai, Ping Wang, Sheng Li, Zhaocheng Xiong, Peifang Chen, Qingyou Liu, Deshun Shi
The present study was undertaken to examine the effect of MG132, a proteasome inhibitor, on the in vitro development, zygotic genome activation (ZGA) and epigenetic modification of Debao porcine somatic cell nuclear transfer (SCNT) embryos. Treatment of oocytes with 1 μM MG132 from 30 h to 42 h of maturation and SCNT embryos with 5 μM MG132 for 2 h after fusion resulted in higher blastocyst yield (36.5%) of SCNT embryos compared with the control group (11.0%). The ZGA of SCNT embryos at 2- and 4-cell stages was also enhanced by MG132 treatment through altering the RNA pol II status and increasing the expression of eIF3A and TFIIA...
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28407690/the-suppressing-effects-of-btg3-expression-on-aggressive-behaviors-and-phenotypes-of-colorectal-cancer-an-in-vitro-and-vivo-study
#12
Hua-Chuan Zheng, Hao-Yu He, Ji-Cheng Wu, Jing Li, Shuang Zhao, Gui-Feng Zhao, Hua-Mao Jiang, Xue-Wen Yu, Zhi-Jie Li
Here, we found that down-regulated expression of BTG3 might be positively correlated with colorectal carcinogenesis and its overexpression suppressed proliferation, glycolysis, mitochondrial respiration, cell cycle progression, migration, and invasion, and induced apoptosis, senescence and differentiation in SW480 and SW620 cells. After treated with cisplatin, MG132, paclitaxel and SAHA, BTG3 transfectants exhibited lower viability and higher apoptosis than the control in both time- and dose-dependent manners...
March 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/28404547/the-role-of-mir-320a-and-il-1%C3%AE-in-human-chondrocyte-degradation
#13
Y Jin, X Chen, Z Y Gao, K Liu, Y Hou, J Zheng
OBJECTIVES: This study aimed to explore the role of miR-320a in the pathogenesis of osteoarthritis (OA). METHODS: Human cartilage cells (C28/I2) were transfected with miR-320a or antisense oligonucleotides (ASO)-miR-320a, and treated with IL-1β. Subsequently the expression of collagen type II alpha 1 (Col2α1) and aggrecan (ACAN), and the concentrations of sulfated glycosaminoglycans (sGAG) and matrix metallopeptidase 13 (MMP-13), were assessed. Luciferase reporter assay, qRT-PCR, and Western blot were performed to explore whether pre-B-cell leukemia Homeobox 3 (PBX3) was a target of miR-320a...
April 2017: Bone & Joint Research
https://www.readbyqxmd.com/read/28392346/uchl1-regulates-melanogenesis-through-controlling-mitf-stability-in-human-melanocytes
#14
Eun Young Seo, Seon-Pil Jin, Kyung-Cheol Sohn, Chi-Hyun Park, Dong Hun Lee, Jin Ho Chung
Ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) is involved in many signaling pathways via the ubiquitin-proteasome system. UCHL1 is expressed in the human skin and serves as a neuronal marker; however, its functions in melanogenesis remain unknown. Here, we investigated the role of UCHL1 in melanogenesis, and elucidated the underlying mechanism using human melanocytes. UCHL1 downregulation by siRNA resulted in upregulation of microphthalmia-associated transcription factor (MITF), tyrosinase, dopachrome tautomerase (DCT), tyrosinase-related protein 1 (TRP-1), and melanin...
April 6, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28388298/nf-%C3%AE%C2%BAb-is-a-potential-molecular-drug-target-in-triple-negative-breast-cancers
#15
Paola Poma, Manuela Labbozzetta, Natale D'Alessandro, Monica Notarbartolo
Breast cancer continues to cause significant burden in global health morbidity and mortality. Triple-negative breast cancers (TNBCs) are highly aggressive with poor prognosis and are characterized by lack of expression of estrogen receptor, progesterone receptor, and human epidermal growth factor receptor (Her-2). TNBCs are often resistant to cytotoxic chemotherapy and pose major difficulty in achieving personalized medicine due to their molecular heterogeneity. There is increasing evidence that the aberrant activation of nuclear factor (NF)-κB signaling is a frequent characteristic of TNBCs...
April 2017: Omics: a Journal of Integrative Biology
https://www.readbyqxmd.com/read/28373900/prevention-of-streptozotocin-induced-diabetic-nephropathy-by-mg132-possible-roles-of-nrf2-and-i%C3%AE%C2%BAb
#16
Lili Kong, Yangwei Wang, Manyu Luo, Yi Tan, Wenpeng Cui, Lining Miao
Our previous study showed that proteasomal inhibitor MG132 can prevent diabetic nephropathy (DN) along with upregulation of nuclear factor (erythroid-derived 2)-like 2 (Nrf2). The present study was to investigate whether MG132 can prevent DN in wild-type and Nrf2-KO mice. Type 1 diabetes was induced in wild-type and Nrf2-KO mice by multiple low doses of streptozotocin. Two weeks after streptozotocin injection, both wild-type and Nrf2-KO mice were randomly divided into four groups: control, MG132, DM, and DM/MG132...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28369599/ring-h2-type-e3-gene-vprh2-from-vitis-pseudoreticulata-improves-resistance-to-powdery-mildew-by-interacting-with-vpgrp2a
#17
Lei Wang, Xiaoqing Xie, Wenkong Yao, Jie Wang, Fuli Ma, Chen Wang, Yazhou Yang, Weihuo Tong, Jianxia Zhang, Yan Xu, Xiping Wang, Chaohong Zhang, Yuejin Wang
Grapevine is one of the world's most important fruit crops. European cultivated grape species have the best fruit quality but show almost no resistance to powdery mildew (PM). PM caused by Uncinula necator is a harmful disease that has a significant impact on the economic value of the grape crop. In this study, we examined a RING-H2-type ubiquitin ligase gene VpRH2 that is associated with significant PM-resistance of Chinese wild-growing grape Vitis pseudoreticulata accession Baihe-35-1. The expression of VpRH2 was clearly induced by U...
March 1, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28369484/nbs1-is-regulated-by-two-kind-of-mechanisms-atm-dependent-complex-formation-with-mre11-and-rad50-and-cell-cycle-dependent-degradation-of-protein
#18
Hui Zhou, Kasumi Kawamura, Hiromi Yanagihara, Junya Kobayashi, Qiu-Mei Zhang-Akiyama
Nijmegen breakage syndrome (NBS), a condition similar to Ataxia-Telangiectasia (A-T), is a radiation-hypersensitive genetic disorder showing chromosomal instability, radio-resistant DNA synthesis, immunodeficiency, and predisposition to malignances. The product of the responsible gene, NBS1, forms a complex with MRE11 and RAD50 (MRN complex). The MRN complex is necessary for the DNA damage-induced activation of ATM. However, the regulation of MRN complex formation is still unclear. Here, we investigated the regulatory mechanisms of MRN complex formation...
March 22, 2017: Journal of Radiation Research
https://www.readbyqxmd.com/read/28363601/methylglyoxal-induced-ampk-activation-leads-to-autophagic-degradation-of-thioredoxin-1-and-glyoxalase-2-in-ht22-nerve-cells
#19
Alcir Luiz Dafre, Ariana Ern Schmitz, Pamela Maher
Methylglyoxal (MGO) is a major glycating agent that reacts with basic residues of proteins and promotes the formation of advanced glycation end products which are believed to play key roles in a number of pathologies, such as diabetes, Alzheimer's disease, and inflammation. We previously showed that MGO treatment targets the thioredoxin and the glyoxalase systems, leading to a decrease in Trx1 and Glo2 proteins in immortalized mouse hippocampal HT22 nerve cells. Here, we propose that autophagy is the underlying mechanism leading to Glo2 and Trx1 loss induced by MGO...
March 29, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28359145/neuroprotective-effects-of-protein-tyrosine-phosphatase-1b-inhibition-against-er-stress-induced-toxicity
#20
Yu-Mi Jeon, Shinrye Lee, Seyeon Kim, Younghwi Kwon, Kiyoung Kim, Chang Geon Chung, Seongsoo Lee, Sung Bae Lee, Hyung-Jun Kim
Several lines of evidence suggest that endoplasmic reticulum (ER) stress plays a critical role in the pathogenesis of many neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis. Protein tyrosine phosphatase 1B (PTP1B) is known to regulate the ER stress signaling pathway, but its role in neuronal systems in terms of ER stress remains largely unknown. Here, we showed that rotenone-induced toxicity in human neuroblastoma cell lines and mouse primary cortical neurons was ameliorated by PTP1B inhibition...
April 2017: Molecules and Cells
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