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https://www.readbyqxmd.com/read/28063052/monitoring-of-chemically-induced-colitis
#1
Sonja Reißig, Benno Weigmann
Inflammation is a common symptom of inflammatory bowel disease (IBD). Actually, many experimental models of colitis exist and try to mimic the human situation in order to understand the pathogenesis of Crohn's disease and ulcerative colitis. These experimental models of inflammation can be characterized by specific parameters, which illustrate the proceeding inflammatory process. By use of these models potentially new reagents for improved therapeutic approaches can be analyzed. Here, we describe the TNBS-mediated colitis model and specify different parameters for the detailed characterization of the inflammatory process in experimental colitis models...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28050757/implication-of-nrf2-ho-1-pathway-in-the-coloprotective-effect-of-coenzyme-q10-against-experimentally-induced-ulcerative-colitis
#2
Ahmed E Khodir, Hoda Atef, Eman Said, Hassan A ElKashef, Hatem A Salem
PURPOSE: Given the increased incidence of ulcerative colitis worldwide, the current study was designed to investigate the coloprotective potential of CoQ10 against experimentally induced ulcerative colitis (UC) and specify the implicated mechanisms. METHODS: Ulcerative colitis was induced by intracolonic instillation of [2 ml, 3% v/v acetic acid (AA)]. Rats in the different experimental groups received CoQ10 (10 or 30 and 100 mg/kg, orally) for eight consecutive days, either in a protective or curative regimen...
January 3, 2017: Inflammopharmacology
https://www.readbyqxmd.com/read/28042320/heparin-improves-bmsc-cell-therapy-anticoagulant-treatment-by-heparin-improves-the-safety-and-therapeutic-effect-of-bone-marrow-derived-mesenchymal-stem-cell-cytotherapy
#3
Li Liao, Bingzheng Shi, Heran Chang, Xiaoxia Su, Lichao Zhang, Chunsheng Bi, Yi Shuai, Xiaoyan Du, Zhihong Deng, Yan Jin
Systemic infusion of bone marrow-derived mesenchymal stem cells (BMSCs) has become a promising strategy for disease treatment and tissue regeneration. Strategies to enhance the efficiency of BMSC cell therapy are crucial to promote its clinical application. Here, we aimed to improve BMSC cell therapy by inhibiting the BMSC-induced coagulation reaction. Intravenous injection of gradient BMSCs into mice showed that BMSCs were not fully compatible with blood. Large doses of BMSCs induced a series of symptoms of respiratory failure and heart failure...
2017: Theranostics
https://www.readbyqxmd.com/read/28039905/ultraviolet-a-eye-irradiation-ameliorates-colon-carcinoma-induced-by-azoxymethane-and-dextran-sodium-sulfate-through-%C3%AE-endorphin-and-methionine-enkephalin
#4
Keiichi Hiramoto, Satoshi Yokoyama, Yurika Yamate
BACKGROUND: We previously reported that ultraviolet (UV) A eye irradiation reduces the ulcerative colitis induced by dextran sodium sulfate (DSS). This study examined the effects of UVA on colon carcinoma induced by azoxymethane (AOM) and DSS. METHODS: We irradiated the eyes of ICR mice with UVA at a dose of 110 kJ/m(2) using an FL20SBLB-A lamp for the experimental period. RESULTS: In mice treated with these drugs, the symptom of colon carcinoma were reduced by UVA eye irradiation...
December 31, 2016: Photodermatology, Photoimmunology & Photomedicine
https://www.readbyqxmd.com/read/28039475/gpr120-a-potential-therapeutic-target-for-experimental-colitis-in-il-10-deficient-mice
#5
Jie Zhao, Honggang Wang, Peiliang Shi, Wenbo Wang, Ye Sun
It has been proved that interleukin-10-knockout (IL-10 KO) mice display the most similar characteristics to that of human Crohn's disease (CD). Docosahexaenoic acid (DHA) has well established beneficial effects on human and animal models health with potent anti-inflammatory effects with poorly understood mechanisms. This study was aimed at figuring out whether DHA could ameliorate the Crohn's colitis by activating GPR120 and whether GPR120 could be a potential therapeutic target for CD.16 week-old mice included in our present study were divided into three groups, WT group, IL-10 KO group and DHA group(IL-10 KO mice with DHA treatment, i...
December 26, 2016: Oncotarget
https://www.readbyqxmd.com/read/28035546/disequilibrium-in-the-cd8-cd28-cd8-cd28-t-lymphocyte-balance-is-related-to-prognosis-in-rats-with-trinitrobenzenesulfonic-acid-induced-colitis
#6
Shixue Dai, Hongxiang Gu, Qianyi Lin, Tiaosi Xing, Minhua Chen, Tao Zhong, Gang Wu, Yanling Feng, Hongbo Liu, Yong Gao, Hongjian Jian, Minhai Zhang, Hongmei Mo, Huanjie Zhu, Dongsheng Chen, Jun Xu, Ying Zou, Honggang Chi, Yuzhen Zhu
PURPOSE: The CD8(+)CD28(+)/CD8(+)CD28(-) T lymphocyte balance is vital for human ulcerative colitis (UC) but has not been defined in experimental colitis. This investigation will try to identify the changes that occur in the CD8(+)CD28(+)/CD8(+)CD28(-) T lymphocyte balance during the progression of trinitrobenzenesulfonic acid (TNBS)-induced colitis in rats. METHODS: The frequencies of blood CD8(+)CD28(+) and CD8(+)CD28(-) T lymphocytes were detected in the rats belonging to the normal, model, and treated groups on five days using flow cytometry...
December 29, 2016: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/28035211/induction-of-indoleamine-2-3-dioxygenase-by-pre-treatment-with-poly-i-c-may-enhance-the-efficacy-of-msc-treatment-in-dss-induced-colitis
#7
Da-Bin Ryu, Ji-Young Lim, Sung-Eun Lee, Gyeongsin Park, Chang-Ki Min
Mesenchymal stem cells (MSCs) have been used experimentally for treating inflammatory disorders, partly owing to their immunosuppressive properties. The goal of the study was to determine whether TLR ligands can enhance the therapeutic efficacy of bone marrow-derived MSCs for the treatment of inflammatory bowel disease. Mice (C57BL6) were administered with 4% dextran sulfate sodium (DSS) in drinking water for 7 days and injected with MSCs on days 1 and 3 following DSS ingestion. Our results demonstrated that among various TLR ligands, MSCs treated with polyinosinic-polycytidylic acid [poly(I:C)], which is a TLR3 ligand, more profoundly induced IDO, which is a therapeutically relevant immunosuppressive factor, without any observable phenotype change in vitro...
December 2016: Immune Network
https://www.readbyqxmd.com/read/28034757/vancomycin-pre-treatment-impairs-tissue-healing-in-experimental-colitis-importance-of-innate-lymphoid-cells
#8
Di Zhao, Chenwen Cai, Qing Zheng, Shuang Jin, Dongjuan Song, Jun Shen, Zhihua Ran
BACKGROUND AND AIMS: The interplay between luminal microbes and innate immunity during colonic epithelial repair has been well noted. At the same time, antibiotic has widely been used during flare-ups of ulcerative colitis. The possible effects of luminal microbiota disruption caused by antibiotics usage on epithelial repairing have been scarcely discussed. Innate lymphoid cells (ILCs) embedded in the lamina propria can be modulated by gut microbes, resulting in altered colonic IL-22/pSTAT3 levels, which is considered a prominent molecular axis in tissue repairing after epithelium damage...
December 27, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28031321/inflammatory-hypoxia-induces-syndecan-2-expression-through-il-1%C3%AE-mediated-foxo3a-activation-in-colonic-epithelia
#9
Sojoong Choi, Heesung Chung, Heejeong Hong, So Yeon Kim, Seong-Eun Kim, Ju-Young Seoh, Chang Mo Moon, Eun Gyeong Yang, Eok-Soo Oh
Chronic inflammation is known to be a key causative factor in tumor progression, but we do not yet fully understand the molecular mechanism through which inflammation leads to cancer. Here, we report that the dextran sulfate sodium (DSS)-induced mouse model of chronic colitis is associated with increases in the serum level of IL-1β and the colonic epithelial expression of the cell-surface heparan sulfate proteoglycan, syndecan-2. We further show that IL-1β stimulated the transcription of syndecan-2 via NF-κB-dependent FOXO3a activation in normal colonic epithelial cells (CCD841CoN cells) and early-stage colon cancer cells (HT29 cells)...
December 28, 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28009291/myeloid-derived-suppressor-cells-are-controlled-by-regulatory-t-cells-via-tgf-%C3%AE-during-murine-colitis
#10
Cho-Rong Lee, Yewon Kwak, Taewoo Yang, Jung Hyun Han, Sang-Heon Park, Michael B Ye, Wongeun Lee, Kyu-Young Sim, Jung-Ah Kang, Yong-Chul Kim, Sarkis K Mazmanian, Sung-Gyoo Park
Myeloid-derived suppressor cells (MDSCs) are well known regulators of regulatory T cells (Treg cells); however, the direct regulation of MDSCs by Treg cells has not been well characterized. We find that colitis caused by functional deficiency of Treg cells leads to altered expansion and reduced function of MDSCs. During differentiation of MDSCs in vitro from bone marrow cells, Treg cells enhanced MDSC function and controlled their differentiation through a mechanism involving transforming growth factor-β (TGF-β)...
December 20, 2016: Cell Reports
https://www.readbyqxmd.com/read/28008786/a-probiotic-combination-attenuates-experimental-colitis-through-inhibition-of-innate-cytokine-production
#11
M S Kim, J S Byun, Y S Yoon, D Y Yum, M J Chung, J C Lee
Inflammatory bowel disease (IBD) is a severe immune cell-mediated syndrome characterised by extensive inflammatory and effector mucosal responses leading to tissue destruction in the colon and small intestine. The leading hypothesis is that dysbiosis of the gut flora causes an excessive immune response and inflammation in the gastrointestinal track. Lactic acid bacteria (LAB) can correct dysbiosis of the normal microbiota. In the current study, the therapeutic potential of seven LAB strains in combination to treat IBD was evaluated using experimental colitis model...
December 23, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27995407/the-anti-inflammatory-effect-and-intestinal-barrier-protection-of-hu210-differentially-depend-on-tlr4-signaling-in-dextran-sulfate-sodium-induced-murine-colitis
#12
Sisi Lin, Yongyu Li, Li Shen, Ruiqin Zhang, Lizhi Yang, Min Li, Kun Li, Jakub Fichna
BACKGROUND: Ulcerative colitis (UC) is strongly associated with inflammation and intestinal barrier disorder. The nonselective cannabinoid receptor agonist HU210 has been shown to ameliorate inflamed colon in colitis, but its effects on intestinal barrier function and extraintestinal inflammation are unclear. AIMS: To investigate the effects and the underlying mechanism of HU210 action on the UC in relation to a role of TLR4 and MAP kinase signaling. METHODS: Wild-type (WT) and TLR4 knockout (Tlr4 (-/-)) mice were exposed to 4% dextran sulfate sodium (DSS) for 7 days...
December 19, 2016: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/27989839/cilostazol-and-enzymatically-modified-isoquercitrin-attenuate-experimental-colitis-and-colon-cancer-in-mice-by-inhibiting-cell-proliferation-and-inflammation
#13
Yumi Kangawa, Toshinori Yoshida, Kiyoshi Maruyama, Minako Okamoto, Tohru Kihara, Michi Nakamura, Masako Ochiai, Yoshitaka Hippo, Shim-Mo Hayashi, Makoto Shibutani
We previously reported the anti-inflammatory effects of cilostazol, a selective inhibitor of phosphodiesterase 3, and two antioxidants, enzymatically modified isoquercitrin and α-lipoic acid in a dextran sodium sulphate-induced colitis mouse model. We further examined the chemopreventive effects of these substances in a murine azoxymethane/dextran sodium sulphate -induced colorectal carcinoma model and compared the effects with those of the well-known anticancer natural plant pigment, anthocyanin. In addition, the effects on cell proliferation activity were evaluated in colon cancer cell lines and mucosal epithelial cells in a model of acute dextran sodium sulphate-induced colitis...
December 15, 2016: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/27983971/immune-protective-effect-of-echinococcosis-on-colitis-experimental-model-is-dependent-of-down-regulation-of-tnf-%C3%AE-and-no-production
#14
Lila Khelifi, Imene Soufli, Moussa Labsi, Chafia Touil-Boukoffa
Hydatid disease (echinococcosis) is a chronic, endemic helminthic disease caused by the larval stage of the tapeworm, Echinococcus granulosus. This disease is endemic in many parts of the world, such as the Mediterranean area, and in particular in Algeria. Helminth parasites have developed complex strategies to modulate the immune responses of their hosts through versatile immune-regulatory mechanisms. These mechanisms may regulate immune responses associated with inflammatory diseases such as inflammatory bowel diseases (IBD)...
February 2017: Acta Tropica
https://www.readbyqxmd.com/read/27982124/the-human-intestinal-microbiota-of-constipated-predominant-irritable-bowel-syndrome-patients-exhibits-anti-inflammatory-properties
#15
Alain P Gobert, Giulia Sagrestani, Eve Delmas, Keith T Wilson, Thomas G Verriere, Michel Dapoigny, Christophe Del'homme, Annick Bernalier-Donadille
The intestinal microbiota of patients with constipated-predominant irritable bowel syndrome (C-IBS) displays chronic dysbiosis. Our aim was to determine whether this microbial imbalance instigates perturbation of the host intestinal mucosal immune response, using a model of human microbiota-associated rats (HMAR) and dextran sulfate sodium (DSS)-induced experimental colitis. The analysis of the microbiota composition revealed a decrease of the relative abundance of Bacteroides, Roseburia-Eubacterium rectale and Bifidobacterium and an increase of Enterobacteriaceae, Desulfovibrio sp...
December 16, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27982094/huangqin-tang-ameliorates-dextran-sodium-sulphate-induced-colitis-by-regulating-intestinal-epithelial-cell-homeostasis-inflammation-and-immune-response
#16
Ying Zou, Jiantao Lin, Wenyang Li, Zhuguo Wu, Zhiwei He, Guoliang Huang, Jian Wang, Caiguo Ye, Xiaoyan Cheng, Congcong Ding, Xuebao Zheng, Honggang Chi
Huangqin-tang (HQT) is a traditional Chinese medicine (TCM) formula widely used for the treatment of inflammatory bowel disease in China. However, the molecular mechanisms by which HQT protects the colon are unclear. We studied the protective effects of HQT and the underlying mechanisms in an experimental mouse model and in vitro. In vivo, dextran sodium sulphate (DSS)-induced acute and chronic colitis were significantly ameliorated by HQT as gauged by phenotypic, histopathologic and inflammatory manifestations of the disease...
December 16, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27981571/haematopoietic-prolyl-hydroxylase-1-deficiency-promotes-m2-macrophage-polarization-and-is-both-necessary-and-sufficient-to-protect-against-experimental-colitis
#17
Sophie Van Welden, Martine De Vos, Ben Wielockx, Simon J Tavernier, Melissa Dullaers, Sara Neyt, Benedicte Descamps, Lindsey Devisscher, Sarah Devriese, Lien Van den Bossche, Tom Holvoet, Ann Baeyens, Carmen Correale, Silvia D'Alessio, Christian Vanhove, Filip De Vos, Bruno Verhasselt, Georg Breier, Bart N Lambrecht, Sophie Janssens, Peter Carmeliet, Silvio Danese, Dirk Elewaut, Debby Laukens, Pieter Hindryckx
Prolyl hydroxylase domain-containing proteins (PHD) regulate the adaptation of cells to hypoxia. Pan-hydroxylase inhibition is protective in experimental colitis in which PHD1 plays a prominent role. However, it is currently unknown how PHD1 targeting regulates this protection and which cell type(s) are involved. Here we demonstrated that Phd1 deletion in endothelial and haematopoietic cells (Phd1(f/)(f) Tie2:cre) protected mice from dextran sulphate sodium (DSS)-induced colitis with reduced epithelial erosions, immune cell infiltration and colonic microvascular dysfunction, whereas the response of Phd2(f/+) Tie2:cre and Phd3(f/)(f) Tie2:cre mice to DSS was similar to their littermate controls...
December 16, 2016: Journal of Pathology
https://www.readbyqxmd.com/read/27978459/le-carbone-a-charcoal-supplement-modulates-dss-induced-acute-colitis-in-mice-through-activation-of-ampk%C3%AE-and-downregulation-of-stat3-and-caspase-3-dependent-apoptotic-pathways
#18
Mst Rejina Afrin, Somasundaram Arumugam, Md Azizur Rahman, Vengadeshprabhu Karuppagounder, Remya Sreedhar, Meilei Harima, Hiroshi Suzuki, Takashi Nakamura, Shizuka Miyashita, Kenji Suzuki, Kazuyuki Ueno, Kenichi Watanabe
Le Carbone (LC) is a charcoal supplement, which contains a large amount of dietary fibers. Several studies suggested that charcoal supplement may be beneficial for stomach disorders, diarrhea, gas and indigestion. But no studies address whether LC intake would suppress inflammation, cell proliferation or disease progression in colitis. In the present study, the effect of LC on experimental colitis induced by dextran sulfate sodium (DSS) in mice and its possible mechanism of action were examined. A study was designed for 8days, using C57BL/6 female mice that were administered with 3% DSS in drinking water for 7days followed by another 1day consumption of normal water with or without treatment...
February 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/27965154/dysfunction-of-hepatic-regulatory-t-cells-in-experimental-sclerosing-cholangitis-is-related-with-il-12-signaling
#19
Dorothee Schwinge, Franziska von Haxthausen, Alexander Quaas, Antonella Carambia, Benjamin Otto, Fabian Glaser, Benedikt Höh, Nina Thiele, Tanja Schoknecht, Samuel Huber, Niklas Steffens, Ansgar W Lohse, Johannes Herkel, Christoph Schramm
BACKGROUND AND AIMS: Reduced numbers of regulatory T cells (Treg) have been reported in patients with primary sclerosing cholangitis (PSC); therefore, Treg expansion might serve as a therapeutic approach. Here, we explored whether treatment with IL-2/ IL-2 monoclonal antibody complex (IL-2/IL-2Ab complex) could provide in vivo Treg expansion and treatment of experimental sclerosing cholangitis. METHODS: Treg were expanded by repeated injection of IL-2/IL-2Ab complex in mouse models of cholangitis (Mdr2(-/-), DDC) or colitis (DSS) as control...
December 10, 2016: Journal of Hepatology
https://www.readbyqxmd.com/read/27959399/abnormalities-in-endocrine-and-immune-cells-are-correlated-in-dextran%C3%A2-sulfate%C3%A2-sodium%C3%A2-induced-colitis-in-rats
#20
Magdy El-Salhy, Jan Gunnar Hatlebakk, Odd Helge Gilja
The interaction between the gut hormones and the immune system has been suggested to serve an important role in the pathophysiology of inflammatory bowel disease. The aims of the present study were to elucidate the possible abnormalities in the colonic endocrine cells in rats with dextran sodium sulfate (DSS)‑induced colitis, and to determine whether they are correlated with alterations in the immune cells. A total of 24 male Wistar rats were divided into two groups: Control and DSS‑induced colitis. Colonic tissues were harvested via postmortem laparotomy from all of the animals at the end of the experimental period, and fixed and sectioned for histology...
January 2017: Molecular Medicine Reports
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