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https://www.readbyqxmd.com/read/28714559/how-we-make-dna-origami
#1
Klaus F Wagenbauer, Floris A S Engelhardt, Evi K Stahl, Vera K Hechtl, Pierre Stömmer, Fabian Seebacher, Letizia Meregalli, Philip Ketterer, Thomas Gerling, Hendrik Dietz
DNA ORIGAMI has attracted substantial attention since its invention ten years ago due to the seemingly infinite possibilities that it affords for creating customized nanoscale objects. While the basic concept of DNA origami is easy to understand, using custom DNA origami in practical applications requires detailed know-how for designing and producing the particles with sufficient quality, and preparing them at appropriate concentrations with the necessary degree of purity in custom environments. Such know-how is not readily available for newcomers to the field, thus slowing down the rate at which new applications outside the field of DNA nanotechnology may emerge...
July 17, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28711679/reproducibility-of-studies-on-text-mining-for-citation-screening-in-systematic-reviews-evaluation-and-checklist
#2
Babatunde Kazeem Olorisade, Pearl Brereton, Peter Andras
CONTEXT: Independent validation of published scientific results through study replication is a pre-condition for accepting the validity of such results. In computation research, full replication is often unrealistic for independent results validation, therefore, study reproduction has been justified as the minimum acceptable standard to evaluate the validity of scientific claims. The application of text mining techniques to citation screening in the context of systematic literature reviews is a relatively young and growing computational field with high relevance for software engineering, medical research and other fields...
July 12, 2017: Journal of Biomedical Informatics
https://www.readbyqxmd.com/read/28710838/enzyme-free-ligation-of-5-phosphorylated-oligodeoxynucleotides-in-a-dna-nanostructure
#3
Markus Kramer, Clemens Richert
Multicomponent reactions are difficult synthetic transformations. For DNA, there is a special opportunity to align multiple strands in a folded nanostructure, so that they are preorganized to give a specific sequence. Multistrand reactions in DNA origami structures have previously been performed using photochemical crosslinking, 1,3-diploar cycloadditions or phosphoramidate-forming reactions. Here we report carbodiimide-driven phosphodiester formation in a small origami sheet that produces DNA strands up to 600 nucleotides in length in a single step...
July 15, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/28698936/creation-of-an-aesthetic-male-nipple-areolar-complex-in-female-to-male-transgender-chest-reconstruction
#4
Cori A Agarwal, Vanessa T Wall, Sagar T Mehta, Daniel P Donato, Norelle K Walzer
BACKGROUND: Female-to-male chest wall reconstruction is becoming more common, but while there is a growing body of the literature describing technique and algorithms, little detail is written on methods for creating a male appearing nipple areolar complex (NAC) from a female NAC utilizing free nipple graft techniques. Incorrect positioning of the NAC on the chest wall and suboptimal shaping and sizing of the NAC are common pitfalls in male NAC creation. METHODS: With this paper, we present techniques for nipple grafting to achieve improved male appearing NACs, as well as a simple, reproducible method for appropriate placement of the NAC relative to the borders of the pectoralis muscle...
July 11, 2017: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/28693541/is-hpa-axis-reactivity-in-childhood-gender-specific-a-systematic-review
#5
REVIEW
Jonneke J Hollanders, Bibian van der Voorn, Joost Rotteveel, Martijn J J Finken
BACKGROUND: In adults, hypothalamus-pituitary-adrenal (HPA) axis activity shows sexual dimorphism, and this is thought to be a mechanism underlying sex-specific disease incidence. Evidence is scarce on whether these sex differences are also present in childhood. In a meta-analysis, we recently found that basal (non-stimulated) cortisol in saliva and free cortisol in 24-h urine follow sex-specific patterns. We explored whether these findings could be extended with sex differences in HPA axis reactivity...
July 11, 2017: Biology of Sex Differences
https://www.readbyqxmd.com/read/28677262/high-resolution-phylogenetic-analysis-of-endomicrobia-reveals-multiple-acquisitions-of-endosymbiotic-lineages-by-termite-gut-flagellates
#6
Aram Mikaelyan, Claire L Thompson, Katja Meuser, Hao Zheng, Pinki Rani, Rudy Plarre, Andreas Brune
Bacteria of the class Endomicrobia form a deep-branching clade in the Elusimicrobia phylum. They are found almost exclusively in the intestinal tract of animals and are particularly abundant in many termites, where they reside as intracellular symbionts in the cellulolytic gut flagellates. Although small populations of putatively free-living lineages have been detected in faunated and flagellate-free hosts, the evolutionary origin of the endosymbionts is obscured by the limited amount of phylogenetic information provided by the 16S rRNA gene fragment amplified with Endomicrobia-specific primers...
July 5, 2017: Environmental Microbiology Reports
https://www.readbyqxmd.com/read/28671890/management-of-infected-mesh-after-abdominal-hernia-repair-systematic-review-and-single-institution-experience
#7
Valeriy Shubinets, Martin J Carney, David L Colen, Michael N Mirzabeigi, Jason M Weissler, Michael A Lanni, Benjamin M Braslow, John P Fischer, Stephen J Kovach
BACKGROUND: Mesh infection after abdominal hernia repair is a devastating complication that affects general and plastic surgeons alike. The purpose of this study was 3-fold: (1) to determine current evidence for treatment of infected abdominal wall mesh via systematic review of literature, (2) to analyze our single-institution experience with treatment of infected mesh patients, and (3) to establish a framework for how to approach this complex clinical problem. METHODS: Literature search was performed using the Preferred Reporting Items for Systematic Reviews and Meta-analysis guidelines, followed by single-institution retrospective analysis of infected mesh patients...
June 1, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28667831/systematic-review-of-immigrant-women-s-experiences-with-perinatal-care-in-north-america
#8
REVIEW
Anika Winn, Erin Hetherington, Suzanne Tough
OBJECTIVE: To understand the perinatal care experiences of pregnant immigrant women in North America. DATA SOURCES: We searched five electronic databases: MEDLINE, PsycINFO, SocINDEX, CINAHL, and Social Work Abstracts. Two categories of search terms, pregnancy and immigrant, were used to conduct a title/abstract and subject heading search. We manually searched the reference lists of all relevant articles to identify additional articles. STUDY SELECTION: Inclusion criteria were qualitative or mixed methods study design, focus on immigrant women's experiences of accessing perinatal care, and data collection in North America...
June 28, 2017: Journal of Obstetric, Gynecologic, and Neonatal Nursing: JOGNN
https://www.readbyqxmd.com/read/28659510/swedish-massage-a-systematic-review-of-its-physical-and-psychological-benefits
#9
Débora M Barreto, Marcus V A Batista
Context • Swedish massage is the systematic application of manual pressure and the movement of soft tissue, with rhythmical pressure and stroking to obtain or maintain health. Studies have revealed its many benefits. Objective • The aim of the current study was to determine the results of past studies that evaluated the benefits of Swedish massage for various populations and to highlight its relevance as an alternative medical practice in health promotion and disease prevention. Methods • The research team performed a review of the literature using the key terms massage, relaxation, and benefits of massage...
April 2017: Advances in Mind-body Medicine
https://www.readbyqxmd.com/read/28658460/-ten-years-of-the-who-framework-convention-on-tobacco-control-progress-in-the-americas
#10
Adriana Blanco, Rosa Carolina Sandoval, Leticia Martínez-López, Roberta de Betânia Caixeta
The objective of this article is to analyze the progress made in the Americas in the implementation of the World Health Organization Framework Convention on Tobacco Control (FCTC) after its tenth anniversary of entry into force. At the time of the analysis, 30 of the 35 countries of the Americas are Parties to the FCTC. While progress has been made in implementing the measures contained in the FCTC, the level of implementation has not been homogeneous either across mandates or across countries. Forty percent of Parties to the Convention in the Americas are yet to implement any of the measures at their highest level of implementation according to the WHO classification...
2017: Salud Pública de México
https://www.readbyqxmd.com/read/28650586/a-complete-thermodynamic-analysis-of-enzyme-turnover-links-the-free-energy-landscape-to-enzyme-catalysis
#11
Hannah Bl Jones, Stephen A Wells, Erica J Prentice, Anthony Kwok, Liyin L Liang, Vickery L Arcus, Christopher R Pudney
Our understanding of how enzymes work is colored by static structure depictions where the enzyme scaffold is presented as either immobile, or in equilibrium between well-defined static conformations. Proteins however exhibit a large degree of motion over a broad range of timescales and magnitudes and this is defined thermodynamically by the enzyme free energy landscape (FEL). The role and importance of enzyme motion is extremely contentious. Much of the challenge is in the experimental detection of so called 'conformational sampling' involved in enzyme turnover...
June 26, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28648580/flowmetry-evolution-in-microvascular-surgery-a-systematic-review
#12
REVIEW
Georgios Pafitanis, Maria Raveendran, Simon Myers, Ali M Ghanem
This review aims to provide a summary of the flowmeter devices used in microvascular surgery and assesses their contribution to improving the clinical outcomes of free tissue transfer. Flowmeters are widely accepted as the standard method of intraoperative assessment of the patency of coronary vascular anastomoses, providing thresholds that predict outcome. There is limited evidence regarding the use of flow measurements in plastic surgery microvascular anastomoses; however, flowmetry appears to have some role in postoperative free flap monitoring and prevention of complications...
May 20, 2017: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/28634701/the-50-most-cited-articles-in-facial-plastic-surgery
#13
Michael T Chang, Zachary G Schwam, Christopher A Schutt, Emily M Kamen, Boris Paskhover
Bibliometric analysis is a common method to determine the most influential articles in medical specialties, as it is an objective measure of peer recognition of scientific work. This study is the first bibliometric analysis of the literature in facial plastic surgery, to determine the most cited papers in the field. Bibliometric analysis was performed using the Science Citation Index from the Institute for Scientific Information, accessed through the Web of Science™. Filter terms relevant to the field of facial plastic surgery were used to identify the 50 most cited journal articles between 1900 and 2016...
June 20, 2017: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/28632322/reduced-intensity-conditioning-allogeneic-transplantation-after-salvage-treatment-with-dt-pace-in-myeloma-patients-relapsing-early-after-autologous-transplant
#14
K Randall, M Kaparou, E Xenou, S Paneesha, B Kishore, A Kanellopoulos, R Lovell, K Holder, J Suhr, L Baker, L Ryan, E Nikolousis
OBJECTIVE: In this retrospective single center study we have looked into the transplant outcomes(overall survival OS, progression free survival PFS,GvHD) and the role of chimerism, DLI and pre-transplant characteristics in patients who had a suboptimal response (<12 months)to an autologous stem cell transplant for myeloma and underwent an Alemtuzumab T-cell depleted reduced intensity allograft(RIC). METHODS: 24 patients were salvaged with 2 cycles of DT-PACE and received a RIC transplant with Fludarabine, Melphalan and Alemtuzumab...
June 20, 2017: European Journal of Haematology
https://www.readbyqxmd.com/read/28619296/understanding-north-american-yoga-therapists-attitudes-skills-and-use-of-evidence-based-practice-a-cross-national-survey
#15
Marlysa Sullivan, Matthew Leach, James Snow, Steffany Moonaz
INTRODUCTION: Little is known about the adoption of evidence-based practice (EBP) by yoga therapists (YTs). OBJECTIVE: To determine the attitudes, skills, training, use, barriers and facilitators to the use of EBP amongst North American YTs DESIGN: Cross-sectional, descriptive survey METHODS: Self-identified YTs practicing in North America were invited to participate in an online survey. YT attitudes, skills, training, utilisation, barriers to use, and facilitators of EBP use were measured using the 84-item Evidence-Based practice Attitude and utilization SurvEy (EBASE)...
June 2017: Complementary Therapies in Medicine
https://www.readbyqxmd.com/read/28567154/angulating-distraction-ulnar-osteotomy-and-interpositional-phosphocalcic-ceramic-wedge-graft-for-a-chronic-monteggia-lesion
#16
Jagadish Prabhu, Mohammed K Faqi, Fahad Al Khalifa, Rashad K Awad
BACKGROUND: Various types of osteotomies have been used to facilitate reduction of the radial head and to prevent recurrent subluxation. The Bouyala technique - open reduction of radial head associated with open wedge ulnar osteotomy with or without annular ligament reconstruction, is presently the most widely used treatment for long- standing traumatic dislocation of the radial head, independently of age, in the absence of osteoarthritis remodeling, and should preferably be performed within 1 year of trauma...
2017: Open Orthopaedics Journal
https://www.readbyqxmd.com/read/28559753/photogrammetric-assessment-of-upper-body-posture-using-postural-angles-a-literature-review
#17
REVIEW
Deepika Singla, Zubia Veqar, Mohammed Ejaz Hussain
OBJECTIVE: The purpose of this paper was to review the literature regarding the measurement properties of various angles used for postural assessment of the head, neck, shoulder, and thorax and to discuss the utility of these measures. METHODS: The inclusion criteria for this literature review were use of postural angles to assess posture, measurement of upper body posture, and research studies conducted in last 3 decades that had free full-text available online entirely in the English language...
June 2017: Journal of Chiropractic Medicine
https://www.readbyqxmd.com/read/28551858/cost-effectiveness-of-drug-treatments-for-advanced-melanoma-a-systematic-literature-review
#18
REVIEW
Darío Rubio-Rodríguez, Silvia De Diego Blanco, Maite Pérez, Carlos Rubio-Terrés
BACKGROUND: Until recently, advanced melanoma (unresectable and metastatic) has had a poor prognosis and has been treated with chemotherapy. The introduction of new treatments (BRAF and MEK inhibitors and immunotherapy) has improved overall survival and progression-free survival of some patients. OBJECTIVE: The objective of this study was to review the published evidence on the cost-effectiveness of pharmacological treatments for advanced melanoma. METHODS: A systematic literature search was conducted, without date or language restrictions, in PubMed, EMBASE, Scopus, the Cochrane Library, the UK National Institute for Health and Care Excellence databases and the Health Technology Assessment journal...
May 27, 2017: PharmacoEconomics
https://www.readbyqxmd.com/read/28531339/limtox-a-web-tool-for-applied-text-mining-of-adverse-event-and-toxicity-associations-of-compounds-drugs-and-genes
#19
Andres Cañada, Salvador Capella-Gutierrez, Obdulia Rabal, Julen Oyarzabal, Alfonso Valencia, Martin Krallinger
A considerable effort has been devoted to retrieve systematically information for genes and proteins as well as relationships between them. Despite the importance of chemical compounds and drugs as a central bio-entity in pharmacological and biological research, only a limited number of freely available chemical text-mining/search engine technologies are currently accessible. Here we present LimTox (Literature Mining for Toxicology), a web-based online biomedical search tool with special focus on adverse hepatobiliary reactions...
May 22, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28526905/effectiveness-of-acellular-dermal-matrix-on-autologous-split-thickness-skin-graft-in-treatment-of-deep-tissue-defect-esthetic-subjective-and-objective-evaluation
#20
Yoo Jung Lee, Myong Chul Park, Dong Ha Park, Hyung Min Hahn, Sue Min Kim, Il Jae Lee
BACKGROUND: A split-thickness skin graft (STSG) is performed to cover a large full-thickness skin defect. Esthetic and functional deficits can result, and many studies have sought to overcome them. This study compared the effectiveness of the acellular dermal matrix (ADM) graft and STSG concerning esthetic and functional effectiveness of ADM on scar quality. METHODS: Of the patients who underwent anterolateral thigh free flap from 2011 to 2015, patients who received skin graft only (n = 10) or skin graft with ADM (n = 20) for coverage of the donor site were enrolled...
May 19, 2017: Aesthetic Plastic Surgery
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