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Immuno sequencing

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https://www.readbyqxmd.com/read/29655625/an-improved-conjugate-vaccine-technology-induction-of-antibody-responses-to-the-tumor-vasculature
#1
Elisabeth J M Huijbers, Judy R van Beijnum, Chung T Lê, Sofya Langman, Patrycja Nowak-Sliwinska, Kevin H Mayo, Arjan W Griffioen
The induction of an antibody response against self-antigens requires a conjugate vaccine technology, where the self-antigen is conjugated to a foreign protein sequence, and the co-application of a potent adjuvant. The choice of this foreign sequence is crucial as a very strong antibody response towards it may compromise the anti-self immune response. Here, we aimed to optimize the conjugate design for application of vaccination against the tumor vasculature, using two different approaches. First, the immunogenicity of the previously employed bacterial thioredoxin (TRX) was reduced by using a truncated from (TRXtr)...
April 11, 2018: Vaccine
https://www.readbyqxmd.com/read/29625247/added-value-of-50-gene-panel-sequencing-to-distinguish-multiple-primary-lung-cancers-from-pulmonary-metastases-a-systematic-investigation
#2
Paul Roepman, Alexandra Ten Heuvel, Karen C Scheidel, Tobias Sprong, Danielle A M Heideman, Cees A Seldenrijk, Gerarda J M Herder, J Alain Kummer
Differentiation between multiple primary lung cancers or pulmonary metastases has important implications for staging, prognosis, and treatment strategies. Clinical and immuno-histopathological criteria have been standardized; however, a substantial number of cases remain difficult to classify. Using next-generation sequencing it is now possible to improve classification of multiple lung cancer lesions. This study systematically investigated the value of routine morphological and immunohistochemical characteristics, p53 protein expression, TP53 mutation analysis, and 50-gene panel sequencing on 111 lesions from 50 patients with multiple lung lesions...
April 3, 2018: Journal of Molecular Diagnostics: JMD
https://www.readbyqxmd.com/read/29619024/extracellular-ngfr-spacers-allow-efficient-tracking-and-enrichment-of-fully-functional-car-t-cells-co-expressing-a-suicide-gene
#3
Monica Casucci, Laura Falcone, Barbara Camisa, Margherita Norelli, Simona Porcellini, Anna Stornaiuolo, Fabio Ciceri, Catia Traversari, Claudio Bordignon, Chiara Bonini, Attilio Bondanza
Chimeric antigen receptor (CAR)-T cell immunotherapy is at the forefront of innovative cancer therapeutics. However, lack of standardization of cellular products within the same clinical trial and lack of harmonization between different trials have hindered the clear identification of efficacy and safety determinants that should be unveiled in order to advance the field. With the aim of facilitating the isolation and in vivo tracking of CAR-T cells, we here propose the inclusion within the CAR molecule of a novel extracellular spacer based on the low-affinity nerve-growth-factor receptor (NGFR)...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29610395/the-future-of-cancer-treatment-using-precision-oncogenomics
#4
Lukas D Wartman
Clinicians should soon have the opportunity to use precision oncogenomics to tailor the optimal cancer treatment to a specific patient. Precision oncogenomics will incorporate different sequencing platforms depending on the goal of the sequencing result. For example, the sequencing strategy used in immuno-oncology for the design of a tumor-specific vaccine may be different than that used by oncologists following a patient for clearance of mutations from circulating tumor DNA in the peripheral blood. I will provide a broad overview of several of the ways that precision oncogenomics is likely to influence the field of oncology over the next several years building off the experience at the Genomics Tumor Board at Washington University in St...
April 2018: Cold Spring Harbor Molecular Case Studies
https://www.readbyqxmd.com/read/29600443/immuno-receptors-from-recognition-to-signaling-and-function
#5
REVIEW
Israel Pecht
The vertebrate adaptive immune response is initiated by specific recognition of antigens. This is carried out by molecules, soluble or cell surface receptors that are members of the Multichain Immune Recognition Receptors (MIRR) group of proteins. The soluble arm of the response is based on antibodies. Kinetic analysis of antibody-antigenic epitope interactions pioneered insights into the complexity underlying the capacity of relatively limited repertoires of antibodies to recognize an essentially unlimited range of epitopes by employing conformational diversity of a given single sequence...
March 29, 2018: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/29567779/mass-spectrometry-assisted-identification-of-adamts13-derived-peptides-presented-on-hla-dr-and-hla-dq
#6
Johana Hrdinová, Fabian C Verbij, Paul H P Kaijen, Robin B Hartholt, Floris van Alphen, Neubury Lardy, Anja Ten Brinke, Karen Vanhoorelbeke, Pooja J Hindocha, Anne S De Groot, Alexander B Meijer, Jan Voorberg, Ivan Peyron
Formation of microthrombi is a hallmark of acquired thrombotic thrombocytopenic purpura. These microthrombi originate from insufficient processing of ultra large von Willebrand factor multimers by ADAMTS13 due to the development of anti-ADAMTS13 autoantibodies. Several studies have identified the major histocompatibility complex class II alleles HLA-DRB1*11, HLA-DQB1*03 and HLA-DQB1*02:02 as risk factors for acquired thrombotic thrombocytopenic purpura development. Previous research in our department indicated that ADAMTS13 CUB2 domain-derived peptides FINVAPHAR and LIRDTHSLR are presented on HLA-DRB1*11 and HLA-DRB1*03, respectively...
March 22, 2018: Haematologica
https://www.readbyqxmd.com/read/29540473/an-activating-mutation-of-interferon-regulatory-factor-4-irf4-in-adult-t-cell-leukemia
#7
Mathew A Cherian, Sydney Olson, Hemalatha Sundaramoorthi, Kitra Cates, Xiaogang Cheng, John Harding, Andrew Martens, Grant A Challen, Manoj Tyagi, Lee Ratner, Daniel Rauch
The human T cell leukemia virus-1 (HTLV-1) oncoprotein Tax drives cell proliferation and resistance to apoptosis early in the pathogenesis of adult T-cell leukemia (ATL). Subsequently, likely as a result of specific immuno-editing, Tax expression is downregulated and functionally replaced by somatic driver mutations of the host genome. Both amplification and point mutations of interferon regulatory factor 4 (IRF4) have been previously detected in ATL, and the K59R mutation is the most common single-nucleotide variation in IRF4 and is found exclusively in ATL...
March 14, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29520448/targeting-the-tumor-microenvironment-with-immunotherapy-for-genitourinary-malignancies
#8
REVIEW
Ariel E Marciscano, Ravi A Madan
Bacillus Calmette-Guérin in urothelial carcinoma, high-dose interleukin-2 in renal cell carcinoma, and sipuleucel-T in prostate cancer serve as enduring examples that the host immune response can be harnessed to promote effective anti-tumor immunity in genitourinary malignancies. Recently, cancer immunotherapy with immune checkpoint inhibitors has transformed the prognostic landscape leading to durable responses in a subset of urothelial carcinoma and renal cell carcinoma patients with traditionally poor prognosis...
March 8, 2018: Current Treatment Options in Oncology
https://www.readbyqxmd.com/read/29486799/radiation-and-pd-l-1-treatment-combinations-immune-response-and-dose-optimization-via-a-predictive-systems-model
#9
Yuri Kosinsky, Simon J Dovedi, Kirill Peskov, Veronika Voronova, Lulu Chu, Helen Tomkinson, Nidal Al-Huniti, Donald R Stanski, Gabriel Helmlinger
BACKGROUND: Numerous oncology combination therapies involving modulators of the cancer immune cycle are being developed, yet quantitative simulation models predictive of outcome are lacking. We here present a model-based analysis of tumor size dynamics and immune markers, which integrates experimental data from multiple studies and provides a validated simulation framework predictive of biomarkers and anti-tumor response rates, for untested dosing sequences and schedules of combined radiation (RT) and anti PD-(L)1 therapies...
February 27, 2018: Journal for Immunotherapy of Cancer
https://www.readbyqxmd.com/read/29467494/alternative-transcription-of-a-shorter-non-anti-angiogenic-thrombospondin-2-variant-in-cancer-associated-blood-vessels
#10
Filip Roudnicky, Sun Young Yoon, Susanna Poghosyan, Simon Schwager, Cedric Poyet, Giorgia Vella, Samia B Bachmann, Sinem Karaman, Jay W Shin, Vivianne I Otto, Michael Detmar
Thrombospondin-2 (TSP2) is an anti-angiogenic matricellular protein that inhibits tumor growth and angiogenesis. Tumor-associated blood vascular endothelial cells (BECs) were isolated from human invasive bladder cancers and from matched normal bladder tissue by immuno-laser capture microdissection. Exon expression profiling analyses revealed a particularly high expression of a short TSP2 transcript containing only the last 9 (3') exons of the full-length TSP2 transcript. Using 5' and 3' RACE (rapid amplification of cDNA ends) and Sanger sequencing, we confirmed the existence of the shorter transcript of TSP2 (sTSP2) and determined its sequence which completely lacked the anti-angiogenic thrombospondin type 1 repeats domain...
February 22, 2018: Oncogene
https://www.readbyqxmd.com/read/29451204/conundrums-and-confusions-regarding-how-polyethylene-glycol-fusion-produces-excellent-behavioral-recovery-after-peripheral-nerve-injuries
#11
REVIEW
George D Bittner, Dale R Sengelaub, Cameron L Ghergherehchi
Current Neuroscience dogma holds that transections or ablations of a segment of peripheral nerves produce: (1) Immediate loss of axonal continuity, sensory signaling, and motor control; (2) Wallerian rapid (1-3 days) degeneration of severed distal axons, muscle atrophy, and poor behavioral recovery after many months (if ever, after ablations) by slowly-regenerating (1 mm/d), proximal-stump outgrowths that must specifically reinnervate denervated targets; (3) Poor acceptance of microsutured nerve allografts, even if tissue-matched and immune-suppressed...
January 2018: Neural Regeneration Research
https://www.readbyqxmd.com/read/29448958/antigenic-and-functional-profiles-of-a-lawsonia-intracellularis-protein-that-shows-a-flagellin-like-trait-and-its-immuno-stimulatory-assessment
#12
Gayeon Won, John Hwa Lee
The obligate intracellular Lawsonia intracellularis (LI), the etiological agent of proliferative enteropathy (PE), is an economically important disease in the swine industry. Due to extreme difficulty of in vitro culture of the pathogen, molecular characterization of protein components of LI that are targets of the immune system, is difficult; thus, the scientific evidence to drive the development of preventive measures is lacking. In this work, we investigated the antigenic and functional characteristics of a putative flagellar-associated protein, LI0570, using in silico computational approaches for epitope prediction and an in vitro protein-based molecular assay...
February 15, 2018: Veterinary Research
https://www.readbyqxmd.com/read/29433542/smurf1-facilitates-estrogen-receptor-%C3%A9-signaling-in-breast-cancer-cells
#13
Huijie Yang, Na Yu, Juntao Xu, Xiaosheng Ding, Wei Deng, Guojin Wu, Xin Li, Yingxiang Hou, Zhenhua Liu, Yan Zhao, Min Xue, Sifan Yu, Beibei Wang, Xiumin Li, Gang Niu, Hui Wang, Jian Zhu, Ting Zhuang
BACKGROUND: Estrogen receptor alpha (ER alpha) is expressed in the majority of breast cancers and promotes estrogen-dependent cancer progression. ER alpha positive breast cancer can be well controlled by ER alpha modulators, such as tamoxifen. However, tamoxifen resistance is commonly observed by altered ER alpha signaling. Thus, further understanding of the molecular mechanisms, which regulates ER alpha signaling, is important to improve breast cancer therapy. METHODS: SMURF1 and ER alpha protein expression levels were measured by western blot, while the ER alpha target genes were measured by real-time PCR...
February 12, 2018: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/29430272/surface-enhanced-raman-scattering-sers-based-biomicrofluidics-systems-for-trace-protein-analysis
#14
REVIEW
Chun-Wei Lee, Fan-Gang Tseng
In recent years, Surface Enhanced Raman Scattering (SERS) has been widely applied to many different areas, including chemical analysis, biomolecule detection, bioagent diagnostics, DNA sequence, and environmental monitor, due to its capabilities of unlabeled fingerprint identification, high sensitivity, and rapid detection. In biomicrofluidic systems, it is also very powerful to integrate SERS based devices with specified micro-fluid flow fields to further focusing/enhancing/multiplexing SERS signals through molecule registration, concentration/accumulation, and allocation...
January 2018: Biomicrofluidics
https://www.readbyqxmd.com/read/29423100/proteomic-profiling-of-antibody-inducing-immunogens-in-tumor-tissue-identifies-psma1-lap3-anxa3-and-maspin-as-colon-cancer-markers
#15
Qian Yang, Michael H Roehrl, Julia Y Wang
We hypothesized that cancer tissue immunogens - antigens capable of inducing specific antibody production in patients - are promising targets for development of precision diagnostics and humoral immunotherapies. We developed an innovative immuno-proteomic strategy and identified new immunogenic markers of colon cancer. Proteins from cancers and matched normal tissues were separated by 2D gel electrophoresis and blotted with serum antibodies from the same patients. Antibody-reactive proteins were sequenced by mass spectrometry and validated by Western blotting and immunohistochemistry...
January 9, 2018: Oncotarget
https://www.readbyqxmd.com/read/29410628/reticuloendotheliosis-virus-inhibits-the-immune-response-acting-on-lymphocytes-from-peripheral-blood-of-chicken
#16
Yulin Bi, Lu Xu, Lingling Qiu, Shasha Wang, Xiangping Liu, Yani Zhang, Yang Chen, Yang Zhang, Qi Xu, Guobin Chang, Guohong Chen
Chicken reticuloendotheliosis virus (REV) causes the atrophy of immune organs and immuno-suppression. The pathogenic mechanisms of REV are poorly understood. The aim of this study was to use RNA sequencing to analyse the effect of REV on immunity and cell proliferation in chicken lymphocytes from peripheral blood in vitro . Overall, 2977 differentially expressed genes (DEGs) were examined from cells between infected with REV or no; 56 DEGs related to cell proliferation and 130 DEGs related to immunity were identified...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29392733/the-role-of-gut-microbiome-and-associated-metabolome-in-the-regulation-of-neuroinflammation-in-multiple-sclerosis-and-its-implications-in-attenuating-chronic-inflammation-in-other-inflammatory-and-autoimmune-disorders
#17
REVIEW
Nicholas Dopkins, Prakash S Nagarkatti, Mitzi Nagarkatti
The importance of the gut microbiome in the regulation of non-infectious diseases has earned recently an unprecedented interest from biomedical researchers. Widespread use of next generation sequencing techniques have prepared a foundation for further research by correlating the presence of specific bacterial species with an onset or severity of a disease state, heralded paradigm-shifting results. This review covers the mechanisms through which a dysbiotic gut microbiota contributes to the pathological symptoms in an autoimmune neurodegenerative disorder, Multiple Sclerosis (MS)...
February 2, 2018: Immunology
https://www.readbyqxmd.com/read/29361649/peptide-selection-for-the-quantification-of-p-iii-np-in-human-serum-by-mass-spectrometry
#18
Danielle Moncrieffe, Mark C Parkin, David Cowan
RATIONALE: Procollagen III amino-terminal propeptide (P-III-NP) is currently monitored in human doping control as a biomarker for growth hormone administration and also in clinical diagnostics using immunoassays. Drawbacks to this approach have been highlighted, and research is ongoing to develop a mass spectrometric method to complement these methods. However, a lack of traceable reference material, the presence of post translational modifications (PTMs), and small blood concentration implicate the development of targeted analytical methods for P-III-NP quantification...
January 23, 2018: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29345757/whole-genome-sequencing-analysis-for-cancer-genomics-and-precision-medicine
#19
REVIEW
Hidewaki Nakagawa, Masashi Fujita
Explosive advances of next-generation sequencer (NGS) and computational analyses have been exploring somatic protein-altered mutations in most cancer types and these coding mutation data are intensively accumulated. However, there is limited information on somatic mutations in non-coding regions including introns, regulatory elements, and non-coding RNAs, structural variants and pathogen in cancer genomes remain widely unexplored. Whole genome sequencing (WGS) approaches can comprehensively explore all types of genomic alterations in cancer and help us to better understand the whole landscape of driver mutations and mutational signature in cancer genomes and elucidate functional or clinical implications of these unexplored genomic regions and mutational signature...
January 18, 2018: Cancer Science
https://www.readbyqxmd.com/read/29333681/myotropic-activity-and-immunolocalization-of-selected-neuropeptides-of-the-burying-beetle-nicrophorus-vespilloides-coleoptera-silphidae
#20
Arkadiusz Urbański, Jan Lubawy, Paweł Marciniak, Grzegorz Rosiński
Burying beetles (Nicrophorus sp.) are necrophagous insects with developed parental care. Genome of Nicrophorus vespilloides has been recently sequenced, which makes them interesting model organism in behavioral ecology. However, we know very little about their physiology, including the functioning of their neuroendocrine system. In this study, one of the physiological activities of proctolin, myosuppressin (Nicve-MS), myoinhibitory peptide (Trica-MIP-5) and the short neuropeptide F (Nicve-sNPF) in N. vespilloides have been investigated...
January 15, 2018: Insect Science
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