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Christian Alflen, Marc Kriege, Irene Schmidtmann, Rüdiger R Noppens, Tim Piepho
BACKGROUND: Orogastric tube placement is a common procedure routinely used in clinical anesthesiology and intensive care medicine. Nevertheless high failure rates and severe complications have been reported. We conducted this study to evaluate if the usage of the new gastric tube guide would speed up the placement of orogastric tubes and ease the procedure. METHODS: Thirty one professionals were given a hands-on-training in orogastric tube placement in a simulation manikin without and with the gastric tube guide...
April 4, 2017: BMC Anesthesiology
Rik Janssens, Anneleen Mortier, Daiane Boff, Pieter Ruytinx, Mieke Gouwy, Bo Vantilt, Olav Larsen, Viktorija Daugvilaite, Mette M Rosenkilde, Marc Parmentier, Sam Noppen, Sandra Liekens, Jo Van Damme, Sofie Struyf, Mauro M Teixeira, Flávio A Amaral, Paul Proost
The chemokine CXCL12 or stromal cell-derived factor 1/SDF-1 attracts hematopoietic progenitor cells and mature leukocytes through the G protein-coupled CXC chemokine receptor 4 (CXCR4). In addition, it interacts with atypical chemokine receptor 3 (ACKR3 or CXCR7) and glycosaminoglycans. CXCL12 activity is regulated through posttranslational cleavage by CD26/dipeptidyl peptidase 4 that removes two NH2-terminal amino acids. CD26-truncated CXCL12 does not induce calcium signaling or chemotaxis of mononuclear cells...
May 15, 2017: Biochemical Pharmacology
Koen Willekens, Geert Reyns, Marjan Diricx, Marc Vanhove, Bernard Noppen, Walter Coudyzer, Yicheng Ni, Jean H M Feyen, Peter Stalmans
Purpose: The purpose of this study was to evaluate the dispersion of intravitreally injected solutions and investigate the influence of varying injection techniques. Methods: This was a prospective study using enucleated porcine eyes and ultra-high-resolution computed tomography (UHRCT) scanning to visualize iomeprol intravitreal dispersion. Sixty eyes were divided over 12 different groups according to the injection procedure: fast (2 seconds) or slow (10 seconds) injection speed and needle tip location (6- and 12-mm needle shaft insertion or premacular tip placement verified by indirect ophthalmoscopy)...
March 1, 2017: Investigative Ophthalmology & Visual Science
T Ott, A Barth, M Kriege, A Jahn-Eimermacher, T Piepho, R R Noppens
BACKGROUND: The novel Totaltrack combines a supraglottic airway device with video laryngoscopic tracheal intubation. The intubation laryngeal mask Fastrach is an established device without visual control of intubation. We hypothesized that supraglottic ventilation success with Totaltrack would be similar to Fastrach, but intubation would be performed faster due to visual control of the procedure. METHODS: Fifty-five anaesthesiologists were randomized into one of two study arms: Fastrach Totaltrack...
April 2017: Acta Anaesthesiologica Scandinavica
T Piepho, T Härer, L Ellermann, R R Noppens
BACKGROUND: Securing the airway in severely ill patients is associated with a high rate of complications. So far, no information exists about the equipment readily available for airway management in German intensive care units (ICUs). It is also unknown if the range of material has improved over time. OBJECTIVES: In the present trial the availability of equipment for airway management in ICUs in Rhineland-Palatinate was evaluated at two different times. MATERIALS AND METHODS: Using a structured questionnaire, all ICUs in the state were contacted in the years 2010 and 2015...
February 13, 2017: Der Anaesthesist
Marc Kriege, Christian Alflen, Johannes Eisel, Thomas Ott, Tim Piepho, Ruediger R Noppens
BACKGROUND: Recent case reports have indicated significant cuff overinflation when using the standard filling volume based on the manufacturer's recommendations in older models of laryngeal tubes. The aim of this study was to determine the minimum cuff pressure needed to perform standardized ventilation without leakage in the new, revised model of the laryngeal tube "LTS-D". METHODS: After ethical approval, LTS-D was placed for ventilation in 60 anesthetized patients...
February 2, 2017: BMC Anesthesiology
Nina Pirlich, Jana A Lohse, Rüdiger R Noppens
A patient's willingness to cooperate is an absolute precondition for successful awake intubation of the trachea. Whilst drug-sedation of patients can jeopardize their spontaneous breathing, topical anesthesia of the airway is a popular technique. The spray-as-you-go technique represents one of the simplest opportunities to anesthetize the airway mucosa. The application of local anesthetic through the working channel of the flexible endoscope is a widespread practice for anesthetists as well as pulmonologists...
January 13, 2017: Journal of Visualized Experiments: JoVE
Belén Martínez-Gualda, Liang Sun, Eva Rivero-Buceta, Aida Flores, Ernesto Quesada, Jan Balzarini, Sam Noppen, Sandra Liekens, Dominique Schols, Johan Neyts, Pieter Leyssen, Carmen Mirabelli, María-José Camarasa, Ana San-Félix
We have recently described a new class of dendrimers with tryptophan (Trp) on the surface that show dual antiviral activities against HIV and EV71 enterovirus. The prototype compound of this family is a pentaerythritol derivative with 12 Trps on the periphery. Here we complete the structure-activity relationship studies of this family to identify key features that might be significant for the antiviral activity. With this aim, novel dendrimers containing different amino acids (aromatic and non-aromatic), tryptamine (a "decarboxylated" analogue of Trp) and N-methyl Trp on the periphery have been prepared...
December 23, 2016: Antiviral Research
Jana Lohse, Rüdiger Noppens
INTRODUCTION: Awake video laryngoscopy is a novel option in airway management that is drawing more and more attention as an alternative to awake endoscopic guided intubation.Main issues: Intubation under preserved spontaneous breathing is the safest method to secure the expected difficult airway. In direct comparisons to awake flexible endoscopic intubation, awake video laryngoscopy achieves satisfactory intubation times and a high acceptance of patients and anesthesiologists. Specific cases, in particular very limited mouth opening or sub-glottic masses, require awake flexible endoscopic intubation...
November 2016: Anästhesiologie, Intensivmedizin, Notfallmedizin, Schmerztherapie: AINS
Shweta Malik, Mariya I Petrova, Nicole C E Imholz, Tine L A Verhoeven, Sam Noppen, Els J M Van Damme, Sandra Liekens, Jan Balzarini, Dominique Schols, Jos Vanderleyden, Sarah Lebeer
To characterize the interaction potential of the human vaginal isolate Lactobacillus plantarum CMPG5300, its genome was mined for genes encoding lectin-like proteins. cmpg5300.05_29 was identified as the gene encoding a putative mannose-binding lectin. Phenotypic analysis of a gene knock-out mutant of cmpg5300.05_29 showed that expression of this gene is important for auto-aggregation, adhesion to the vaginal epithelial cells, biofilm formation and binding to mannosylated glycans. Purification of the predicted lectin domain of Cmpg5300...
November 17, 2016: Scientific Reports
Leon H Oosterik, Huruma N Tuntufye, Jessica Tsonos, Tom Luyten, Sam Noppen, Sandra Liekens, Rob Lavigne, Patrick Butaye, Bruno M Goddeeris
Avian pathogenic Escherichia coli (APEC) are responsible for significant economic losses in the poultry industry. In this study, a model for investigating the pathogenesis of APEC infections was established. APEC strain CH2 (O78) was marked with the luciferase operon (luxCDABE) using a Tn7 transposon and tissues of experimentally infected chickens were analysed for a correlation between the bioluminescent signal and the number of bacteria. Transposition of the lux operon into the chromosome of the APEC isolate did not affect sensitivity to lytic bacteriophages and there was no effect on virulence in an intratracheal infection model in 1-day-old chicks, although results with a subcutaneous infection model were inconclusive...
October 2016: Veterinary Journal
Marc Kriege, Tim Piepho, Susanne Zanker, Christian Alflen, Florian Heid, Rüdiger R Noppens
BACKGROUND: Second-generation laryngeal masks with gastric access are increasingly used in daily practice and expand the indications for laryngeal masks in the OR. Only limited data exist comparing different types of laryngeal masks. We investigated the second-generation laryngeal masks LMA Supreme™ and Ambu® AuraGain™ in a clinical setting. We hypothesized that the two devices would be comparable in terms of success rate and airway complications. METHODS: After approval from the local ethics committee, data were collected in a prospective trial...
February 2017: Minerva Anestesiologica
Sebastian A Wirz, Claudine A Blum, Philipp Schuetz, Werner C Albrich, Christoph Noppen, Beat Mueller, Mirjam Christ-Crain, Philip E Tarr
In a double-blind, randomised, placebo-controlled trial of hospitalised patients with community-acquired pneumonia (CAP), we demonstrated shorter time to clinical stability (TTCS) with adjunct corticosteroid therapy compared with placebo.We did a pre-planned, exploratory analysis of any association between microbiological diagnosis, antibiotic treatment and procalcitonin level and effect of prednisone on TTCS, mortality, and CAP complications (n=726 participants, enrolled between December 2009 and May 2014)...
October 2016: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
María-Dolores Canela, Sam Noppen, Oskía Bueno, Andrea E Prota, Katja Bargsten, Gonzalo Sáez-Calvo, María-Luisa Jimeno, Mohammed Benkheil, Domenico Ribatti, Sonsoles Velázquez, María-José Camarasa, J Fernando Díaz, Michel O Steinmetz, Eva-María Priego, María-Jesús Pérez-Pérez, Sandra Liekens
We investigated the microtubule-destabilizing, vascular-targeting, anti-tumor and anti-metastatic activities of a new series of chalcones, whose prototype compound is (E)-3-(3''-amino-4''-methoxyphenyl)-1-(5'-methoxy-3',4'-methylendioxyphenyl)-2-methylprop-2-en-1-one (TUB091). X-ray crystallography showed that these chalcones bind to the colchicine site of tubulin and therefore prevent the curved-to-straight structural transition of tubulin, which is required for microtubule formation. Accordingly, TUB091 inhibited cancer and endothelial cell growth, induced G2/M phase arrest and apoptosis at 1-10 nM...
February 28, 2017: Oncotarget
N Pirlich, J A Lohse, I Schmidtmann, N Didion, T Piepho, R R Noppens
We compared the Enk Fiberoptic Atomizer Set(™) with boluses of topical anaesthesia administered via the working channel during awake fibreoptic tracheal intubation in 96 patients undergoing elective surgery. Patients who received topical anaesthesia via the atomiser, compared with boluses via the fibreoptic scope, reported a better median (IQR [range]) level of comfort: 1 (1-3 [1-10]) vs. 4 (2-6 [1-10]), p < 0.0001; experienced a reduced total number of coughs: 6 (3-10 [0-34]) vs. 11 (6-13 [0-25]), p = 0...
July 2016: Anaesthesia
Kathrin Reiser, Leen Mathys, Sophie Curbo, Christophe Pannecouque, Sam Noppen, Sandra Liekens, Lars Engman, Mathias Lundberg, Jan Balzarini, Anna Karlsson
BACKGROUND: The entry of HIV into its host cell is an interesting target for chemotherapeutic intervention in the life-cycle of the virus. During entry, reduction of disulfide bridges in the viral envelope glycoprotein gp120 by cellular oxidoreductases is crucial. The cellular thioredoxin reductase-1 plays an important role in this oxidoreduction process by recycling electrons to thioredoxin-1. Therefore, thioredoxin reductase-1 inhibitors may inhibit gp120 reduction during HIV-1 entry...
2016: PloS One
Giorgia Oliviero, Mariano Stornaiuolo, Valentina D'Atri, Fabrizia Nici, Ali Munaim Yousif, Stefano D'Errico, Gennaro Piccialli, Luciano Mayol, Ettore Novellino, Luciana Marinelli, Paolo Grieco, Alfonso Carotenuto, Sam Noppen, Sandra Liekens, Jan Balzarini, Nicola Borbone
By using a new rapid screening platform set on molecular docking simulations and fluorescence quenching techniques, three new anti-HIV aptamers targeting the viral surface glycoprotein 120 (gp120) were selected, synthesized, and assayed. The use of the short synthetic fluorescent peptide V35-Fluo mimicking the V3 loop of gp120, as the molecular target for fluorescence-quenching binding affinity studies, allowed one to measure the binding affinities of the new aptamers for the HIV-1 gp120 without the need to obtain and purify the full recombinant gp120 protein...
February 16, 2016: Analytical Chemistry
Florian Heid, Carolin Bender, Hendrik Gervais, Joachim Schmeck, Wolfgang Kohnen, Rüdiger Noppens
This single-center study prospectively assessed the microbial contamination of anesthetic syringes handled perioperatively under different conditions. We documented high rates of bacterial contamination, with strong but statistically nonsignificant differences between handling groups. Our results identify skin contact as the main source of contamination, and thus we emphasize the impact of proper hand hygiene.
March 1, 2016: American Journal of Infection Control
Vincent Vanheule, Peter Vervaeke, Anneleen Mortier, Sam Noppen, Mieke Gouwy, Robert Snoeck, Graciela Andrei, Jo Van Damme, Sandra Liekens, Paul Proost
Chemokines attract leukocytes to sites of infection in a G protein-coupled receptor (GPCR) and glycosaminoglycan (GAG) dependent manner. Therefore, chemokines are crucial molecules for proper functioning of our antimicrobial defense mechanisms. In addition, some chemokines have GPCR-independent defensin-like antimicrobial activities against bacteria and fungi. Recently, high affinity for GAGs has been reported for the positively charged COOH-terminal region of the chemokine CXCL9. In addition to CXCL9, also CXCL12γ has such a positively charged COOH-terminal region with about 50% positively charged amino acids...
January 15, 2016: Biochemical Pharmacology
Eva Rivero-Buceta, Paula Carrero, Elena Casanova, Elisa G Doyagüez, Andrés Madrona, Ernesto Quesada, María Jesús Peréz-Pérez, Raquel Mateos, Laura Bravo, Leen Mathys, Sam Noppen, Evgeny Kiselev, Christophe Marchand, Yves Pommier, Sandra Liekens, Jan Balzarini, María José Camarasa, Ana San-Félix
The glycoprotein gp120 of the HIV-1 viral envelope has a high content in mannose residues, particularly α-1,2-mannose oligomers. Compounds that interact with these high-mannose type glycans may disturb the interaction between gp120 and its (co)receptors and are considered potential anti-HIV agents. Previously, we demonstrated that a tripodal receptor (1), with a central scaffold of 1,3,5-triethylbenzene substituted with three 2,3,4-trihydroxybenzoyl groups, selectively recognizes α-1,2-mannose polysaccharides...
December 1, 2015: European Journal of Medicinal Chemistry
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