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https://www.readbyqxmd.com/read/28092721/use-of-maldi-tof-mass-spectrometry-in-the-battle-against-bacterial-infectious-diseases-recent-achievements-and-future-perspectives
#1
Vassilios Sandalakis, Ioannis Goniotakis, Iosif Vranakis, Dimosthenis Chochlakis, Anna Psaroulaki
Advancements in microbial identification occur increasingly faster as more laboratories explore, refine and extend the use of mass spectrometry in the field of microbiology. Areas covered: This review covers the latest knowledge found in the literature for quick identification of various classes of bacterial pathogens known to cause human infection by the use of MALDI-TOF MS technology. Except for identification of bacterial strains, more researchers try to "battle time" in favor of the patient. These novel approaches to identify bacteria directly from clinical samples and even determine antibiotic resistance are extensively revised and discussed...
January 16, 2017: Expert Review of Proteomics
https://www.readbyqxmd.com/read/28018582/carriage-of-esbl-ampc-producing-or-ciprofloxacin-non-susceptible-escherichia-coli-and-klebsiella-spp-in-healthy-people-in-norway
#2
Charlotte R Ulstad, Margrete Solheim, Sophie Berg, Morten Lindbæk, Ulf R Dahle, Astrid L Wester
BACKGROUND: Asymptomatic carriage has been recognised as an important risk factor for infection caused by antibiotic resistant bacteria. A 14% global prevalence of Extended-Spectrum Beta-lactamase (ESBL) carriage was recently reported, but large intra-and interregional variations were observed. We investigated the faecal carriage rates of ESBL-, AmpC-producing and ciprofloxacin non-susceptible Escherichia coli and Klebsiella spp. in healthy Norwegians. METHODS: Rectal samples were obtained from 284 volunteers, together with demographic data and information on recent travel history...
2016: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/27942042/faecal-carriage-of-gram-negative-multidrug-resistant-bacteria-among-patients-hospitalized-in-two-centres-in-ulaanbaatar-mongolia
#3
Bayaraa Baljin, Ganbaatar Baldan, Battogtokh Chimeddorj, Khosbayar Tulgaa, Batbaatar Gunchin, Tsogtsaikhan Sandag, Klaus Pfeffer, Colin R MacKenzie, Andreas F Wendel
Gram-negative multidrug-resistant organisms (GN-MDRO) producing β-lactamases (ESBL, plasmid-mediated AmpC β-lactamases and carbapenemases) are increasingly reported throughout Asia. The aim of this surveillance study was to determine the rate of bacterial colonization in patients from two hospitals in the Mongolian capital Ulaanbaatar. Rectal swabs were obtained from patients referred to the National Traumatology and Orthopaedics Research Centre (NTORC) or the Burn Treatment Centre (BTC) between July and September 2014, on admission and again after 14 days...
2016: PloS One
https://www.readbyqxmd.com/read/27889878/same-day-identification-and-antibiotic-susceptibility-testing-on-positive-blood-cultures-a-simple-and-inexpensive-procedure
#4
K Maelegheer, E Nulens
Fast diagnostic tools are becoming a hot topic in microbiology, especially in the case of septic patients. Therefore, we attempted to develop a fast, inexpensive, accurate and easy method to identify bacteria and perform an antibiotic susceptibility test directly on positive blood cultures that could be used in a routine laboratory. A procedure based on centrifugation and washing steps was performed on 110 non-duplicated (including nine seeded) positive blood culture bottles. Direct identification (DID) and antimicrobial susceptibility testing (AST) was conducted on the pellet with the MALDI Biotyper and Phoenix, respectively...
November 26, 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27888481/rapid-bacteria-identification-from-environmental-mining-samples-using-maldi-tof-ms-analysis
#5
Ingrid Regina Avanzi, Louise Hase Gracioso, Marcela Dos Passos Galluzzi Baltazar, Bruno Karolski, Elen Aquino Perpetuo, Claudio Augusto Oller do Nascimento
Copper mining has polluted soils and water, causing a reduction of the microbial diversity and a change in the structure of the resident bacterial communities. In this work, selective isolation combined with MALDI-TOF MS and the 16S rDNA method were used for characterizing cultivable bacterial communities from copper mining samples. The results revealed that MALDI-TOF MS analysis can be considered a reliable and fast tool for identifying copper-resistant bacteria from environmental samples at the genera level...
November 25, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27866257/detection-of-methicillin-resistant-staphylococcus-aureus-using-phage-amplification-combined-with-matrix-assisted-laser-desorption-ionization-mass-spectrometry
#6
Jon C Rees, John R Barr
Antibiotic resistance continues to contribute significantly to morbidity and mortality across the world. Developing new tests for antibiotic-resistant bacteria is a core action to combat resistant infections. We describe a method that uses phage amplification detection (PAD) combined with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) to rapidly identify Staphylococcus aureus and determine phenotypic susceptibility to cefoxitin. Samples tested for S. aureus are incubated together with bacteriophage in the presence and absence of cefoxitin and subjected to rapid trypsin digestion followed by MALDI-MS analysis...
November 19, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27836381/universal-protocol-for-the-rapid-automated-detection-of-carbapenem-resistant-gram-negative-bacilli-directly-from-blood-cultures-by-matrix-assisted-laser-desorption-ionisation-time-of-flight-mass-spectrometry-maldi-tof-ms
#7
Marina Oviaño, Katrin Sparbier, Maria José Barba, Markus Kostrzewa, Germán Bou
Detection of carbapenemase-producing bacteria directly from blood cultures is a major challenge, as patients with bacteraemia are critically ill. Early detection can be helpful for selection of the most appropriate antibiotic therapy as well as adequate control of outbreaks. In the current study, a novel matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF)-based method was developed for the rapid, automated detection of carbapenemase-producing Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter baumannii directly from blood cultures...
December 2016: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/27826358/use-of-molecular-typing-to-investigate-bacterial-translocation-from-the-intestinal-tract-of-chlorpyrifos-exposed-rats
#8
Claire Joly Condette, Bertin Elion Dzon, Farida Hamdad, Maurice Biendo, Véronique Bach, Hafida Khorsi-Cauet
BACKGROUND: Human are confronted on a daily basis with contaminant pesticide residues in food, water and other components of the environment. Although the digestive system is the first organ to come into contact with food contaminants, very few data are available on the impact of low-dose pesticide exposure during the in utero and postnatal periods on intestinal bacterial translocation (BT). Previous studies have revealed that chlorpyrifos (CPF) exposure is associated with intestinal dysbiosis and the contamination of sterile organs...
2016: Gut Pathogens
https://www.readbyqxmd.com/read/27794265/molecular-epidemiology-of-environmental-and-clinical-carbapenemase-producing-gram-negative-bacilli-from-hospitals-in-guelma-algeria-multiple-genetic-lineages-and-first-report-of-oxa-48-in-enterobacter-cloacae
#9
Widad Bouguenoun, Sofiane Bakour, Ahmed Aimen Bentorki, Charbel Al Bayssari, Tarek Merad, Jean-Marc Rolain
This study was designed to investigate environmental colonisation in Algerian hospitals by carbapenem-resistant Gram-negative bacilli (GNB), including molecular characterisation of their resistance, and to perform a comparative molecular analysis between clinical and environmental strains. GNB isolated from hospitalised patients and the hospital environment were identified using microbiological methods and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS). Antibiotic susceptibility testing was performed by disk diffusion and Etest methods...
December 2016: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/27790857/a-self-immobilizing-and-fluorogenic-probe-for-%C3%AE-lactamase-detection
#10
Wuyu Mao, Lingying Xia, Yaqun Wang, Hexin Xie
The spread of antibiotic resistance in pathogenic bacteria has become one of the major concerns to public health. Improved monitoring of drug resistance is of high importance for infectious disease control. One of the major mechanisms for bacteria to overcome treatment of antibiotics is the production of β-lactamases, which are enzymes that hydrolyze the β-lactam ring of the antibiotic. In this study, we have developed a self-immobilizing and fluorogenic probe for the detection of β-lactamase activity. This fluorogenic reagent, upon activation by β-lactamases, turns on a fluorescence signal and, more importantly, generates a covalent linkage to the target enzymes or the nearby proteins...
December 19, 2016: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/27726502/resistance-in-bacteria-and-indirect-beta-lactamase-detection-in-e-coli-isolated-from-culex-pipiens-detected-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#11
Lukáš Hleba, Vladimír Kmeť, Tomáš Tóth, Miroslava Kačániová
The aim of this study was detections of antibiotic resistance and resistance mechanism in bacteria isolated from mosquitos (Culex pipiens) living near humans. Therefore, antibiotic resistance in bacteria isolated from Culex pipiens was investigated by disk diffusion test and MIC E-test in this study. MALDI-TOF mass spectrometry was used for detection of resistant mechanism. In this study, hydrolytic breakdown products after a few hours of incubation of the bacteria isolated from Culex pipiens were detected...
January 2, 2017: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/27707676/erysipelothrix-rhusiopathiae-bacteremia-a-challenging-diagnosis
#12
Maïté Micaelo, Pascal Rasmy, Marlène Amara, Juliette Lambert, Aymeric Coutard, Béatrice Pangon
Erysipelothrix rhusiopathiae, a Gram-positive bacillus, is reported to cause for cutaneous infections and endocarditis. We report a case of E. rhusiopathiae bacteremia without severe clinical illness. The patient, a 74-year-old man, is suffering from a chronic lymphoid leukemia (LLC). Following a trauma, the patient developed a bruise on the left inch. Because the site of shock seemed clinically infected, oral amoxicilline-acid clavulanic (AAC) treatment was started after withdrawn 1 set of blood cultures. These blood culture specimens yielded a Gram-positive bacillus identified as E...
October 1, 2016: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/27703855/accuracy-of-conventional-identification-methods-used-for-enterobacteriaceae-isolates-in-three-nigerian-hospitals
#13
Christiana Jesumirhewe, Peter Oladejo Ogunlowo, Mitsan Olley, Burkhard Springer, Franz Allerberger, Werner Ruppitsch
BACKGROUND: Enterobacteriaceae are ubiquitously present in nature and can be found in the intestinal tract of humans and animals as commensal flora. Multidrug-resistant Enterobacteriaceae are increasingly reported and are a threat to public health implicating a need for accurate identification of the isolates to species level. In developing countries, identification of bacteria basically depends on conventional methods: culture and phenotypic methods that hamper the accurate identification of bacteria...
2016: PeerJ
https://www.readbyqxmd.com/read/27688001/evaluation-of-bacteriocinogenic-activity-safety-traits-and-biotechnological-potential-of-fecal-lactic-acid-bacteria-lab-isolated-from-griffon-vultures-gyps-fulvus-subsp-fulvus
#14
Sara Arbulu, Juan J Jiménez, Loreto Gútiez, Cristina Campanero, Rosa Del Campo, Luis M Cintas, Carmen Herranz, Pablo E Hernández
BACKGROUND: Lactic acid bacteria (LAB) are part of the gut microbiota and produce ribosomally synthesized antimicrobial peptides or bacteriocins with interest as natural food preservatives and therapeutic agents. Bacteriocin-producing LAB are also attractive as probiotics. Griffon vultures (Gyps fulvus subspecies fulvus) are scavenger birds that feed almost exclusively on carrion without suffering apparent ill effects. Therefore, griffon vultures might be considered a reservoir of bacteriocin-producing lactic acid bacteria (LAB) with potential biotechnological applications...
September 29, 2016: BMC Microbiology
https://www.readbyqxmd.com/read/27676238/oxidative-dna-damage-and-repair-in-the-radioresistant-archaeon-thermococcus-gammatolerans
#15
Ewa Barbier, Arnaud Lagorce, Amine Hachemi, Murielle Dutertre, Aurore Gorlas, Lucie Morand, Christine Saint-Pierre, Jean-Luc Ravanat, Thierry Douki, Jean Armengaud, Didier Gasparutto, Fabrice Confalonieri, Jean Breton
The hyperthermophilic archaeon Thermococcus gammatolerans can resist huge doses of γ-irradiation, up to 5.0 kGy, without loss of viability. The potential to withstand such harsh conditions is probably due to complementary passive and active mechanisms, including repair of damaged chromosomes. In this work, we documented the formation and repair of oxidative DNA lesions in T. gammatolerans. The basal level of the oxidized nucleoside, 8-oxo-2'-deoxyguanosine (8-oxo-dGuo), was established at 9.2 (± 0.9) 8-oxo-dGuo per 10(6) nucleosides, a higher level than those usually measured in eukaryotic cells or bacteria...
November 21, 2016: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/27672388/uv-b-radiation-stress-causes-alterations-in-whole-cell-protein-profile-and-expression-of-certain-genes-in-the-rice-phyllospheric-bacterium-enterobacter-cloacae
#16
Jay Kumar, Piyoosh K Babele, Divya Singh, Ashok Kumar
Among the different types of UV radiation, UV-B radiation (280-315 nm) has gained much attention mainly due to its increasing incidence on the Earth's surface leading to imbalances in natural ecosystems. This study deals with the effects of UV-B radiation on the proteome and gene expression in a rice phyllospheric bacterium, Enterobacter cloacae. Of the five bacteria isolated from rice leaves, E. cloacae showed the highest level of resistance to UV-B and total killing occurred after 8 h of continuous exposure to UV-B...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27629893/evaluation-of-a-semiquantitative-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-method-for-rapid-antimicrobial-susceptibility-testing-of-positive-blood-cultures
#17
Jette S Jung, Christina Hamacher, Birgit Gross, Katrin Sparbier, Christoph Lange, Markus Kostrzewa, Sören Schubert
With the increasing prevalence of multidrug-resistant Gram-negative bacteria, rapid identification of the pathogen and its individual antibiotic resistance is crucial to ensure adequate antiinfective treatment at the earliest time point. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry for the identification of bacteria directly from the blood culture bottle has been widely established; however, there is still an urgent need for new methods that permit rapid resistance testing...
November 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27602434/sensitivity-to-enterocins-of-thermophilic-campylobacter-spp-from-different-poultry-species
#18
Jana Ščerbová, Andrea Lauková
Campylobacter spp. have been isolated from various animals, including poultry. They are rapidly transmitted throughout broiler sheds by the fecal-oral route. A promising strategy to reduce Campylobacter spp. in poultry may be done due to the beneficial properties of probiotic bacteria and their bacteriocins. In this study, inhibition spectrum/activity of different enterocins was evaluated against Campylobacter spp. (isolated from different poultry) to indicate further practical use of enterocins. Enterocins are antimicrobial proteinaceous substances produced mostly by enterococci...
December 2016: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/27598775/low-enteric-colonization-with-multidrug-resistant-pathogens-in-soldiers-returning-from-deployments-experience-from-the-years-2007-2015
#19
Hagen Frickmann, Dorothea Wiemer, Claudia Frey, Ralf Matthias Hagen, Rebecca Hinz, Andreas Podbielski, Thomas Köller, Philipp Warnke
This assessment describes the enteric colonization of German soldiers 8-12 weeks after returning from mostly but not exclusively subtropical or tropical deployment sites with third-generation cephalosporin-resistant Enterobacteriaceae, vancomycin-resistant enterococci (VRE), and methicillin-resistant Staphylococcus aureus (MRSA). Between 2007 and 2015, 828 stool samples from returning soldiers were enriched in nonselective broth and incubated on selective agars for Enterobacteriaceae expressing extended-spectrum beta-lactamases (ESBL), VRE and MRSA...
2016: PloS One
https://www.readbyqxmd.com/read/27552433/bacterial-diversity-and-antibiotic-resistances-of-abundant-aerobic-culturable-bacteria-in-input-and-output-samples-of-15-german-biogas-plants
#20
T Schauss, T K Wings, J S Brunner, S P Glaeser, W Dott, P Kämpfer
AIM: Determination of the diversities and antibiotic resistances of the most abundant aerobic culturable bacteria and their survival in 15 German biogas plants (BGPs). METHODS AND RESULTS: Microbiological standard media (complex and selective media) were applied to enumerate mesophilic and oligotrophic bacteria (Enterobacteriaceae, Salmonella, staphylococci and enterococci) in input and output samples of BGPs. Concentrations of aerobic culturable bacteria in outputs were mostly one to two orders of magnitude lower than in input samples...
December 2016: Journal of Applied Microbiology
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