keyword
MENU ▼
Read by QxMD icon Read
search

Maldi bacteria resistance

keyword
https://www.readbyqxmd.com/read/29225047/maldi-tof-ms-analysis-as-a-useful-tool-for-an-identification-of-legionella-pneumophila-a-facultatively-pathogenic-bacterium-interacting-with-free-living-amoebae-a-case-study-from-water-supply-system-of-hospitals-in-bratislava-slovakia
#1
Katarína Trnková, Martina Kotrbancová, Margita Špaleková, Miriam Fulová, Jana Boledovičová, Matej Vesteg
Legionellae, i.e. Legionella pneumophila, are human bacterial hydrophilic facultative pathogens causing pneumonia (Legionnaires' disease). Free-living amoebae (FLA) can serve as natural hosts and thus as reservoirs of many amoebae-resistant bacteria. An encysted amoeba can contribute to the resistance of intracellular L. pneumophila to various chemical and physical treatments. Humans can be infected by droplets containing bacteria from an environmental source or human-made devices such as shower heads, bathtubs, air-conditioning units or whirlpools...
December 7, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/29203405/inactivation-of-arn-operon-and-loss-of-aminoarabinose-on-lipopolysaccharide-as-the-cause-of-susceptibility-to-colistin-in-an-atypical-clinical-isolate-of-proteus-vulgaris
#2
Sophie Baron, Zineb Leulmi, Claude Villard, Abiola Olumuyiwa Olaitan, Amar A Telke, Jean-Marc Rolain
Colistin has become a last-line antibiotic for the treatment of multidrug resistant bacterial infections, but resistance to colistin has emerged in recent years. Some bacteria, such as Proteus sp. or Serratia sp., are intrinsically resistant to colistin but the exact mechanism of this resistance is not known. Here, we have identified the molecular support for the intrinsic colistin resistance in Proteus by comparative genomic transcriptomic and proteomic analysis of colistin susceptible (CSUR P1868_S) strain and a colistin resistant (CSUR P1867_R) strain of an atypical Proteus vulgaris...
December 1, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29188069/simultaneous-isolation-of-chryseobacterium-gleum-from-bloodstream-and-respiratory-tract-first-case-report-from-india
#3
Vidhi Jain, Nayani Amrin Fatema Afzal Hussain, Tasneem Siddiqui, Chinmoy Sahu, Malay Ghar, Kashi Nath Prasad
Introduction. Species of the genus Chryseobacterium are emerging healthcare-associated pathogens, often colonizing the hospital environment. There are no clear guidelines available for antimicrobial susceptibility of this organism. In this report we present the first case, to our knowledge, of simultaneous central-line-associated bloodstream infection (CLABSI) and ventilator-associated pneumonia (VAP) due to Chryseobacterium gleum from India. Case presentation. A 62 years old man with a history of a road traffic accident 1 month previously was referred to our center for further management...
October 2017: JMM Case Reports
https://www.readbyqxmd.com/read/29175433/detection-of-ampc-%C3%AE-lactamase-producing-gram-negative-bacteria-by-maldi-tof-ms-analysis
#4
Cong Li, Shoufu Ding, Ying Huang, Zhongxin Wang, Jilu Shen, Huazhi Ling, Yuanhong Xu
BACKGROUND: Rapid detection of AmpC-producing strains of Gram-negative bacteria is beneficial for patient care. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a novel method that has demonstrated the resistance of a strain to β-lactam antibiotics by determining the molecular structure of an antibiotic and its degradation products. AIM: To study the detection of AmpC-producing Gram-negative bacteria by MALDI-TOF MS and to determine if the method can be used in clinical practice...
November 22, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/29146249/composition-of-lactic-acid-bacteria-during-spontaneous-curly-kale-brassica-oleracea-var-sabellica-fermentation
#5
Magdalena Michalak, Klaudia Gustaw, Adam Waśko, Magdalena Polak-Berecka
The present work is the first report on spontaneous fermentation of curly kale and characteristics of autochthonous lactic acid bacteria (LAB). Our results indicate that curly kale fermentation is the new possibility of the technological use of this vegetable. Bacteria representing ten different species were isolated from three phases of curly kale fermentation and identified by MALDI-TOF mass spectrometry and 16S rRNA gene sequencing. Among them, four species were identified as Lactobacillus spp. (Lb. plantarum 332, Lb...
January 2018: Microbiological Research
https://www.readbyqxmd.com/read/29112122/identification-and-antimicrobial-susceptibility-testing-of-anaerobic-bacteria-rubik-s-cube-of-clinical-microbiology
#6
REVIEW
Márió Gajdács, Gabriella Spengler, Edit Urbán
Anaerobic bacteria have pivotal roles in the microbiota of humans and they are significant infectious agents involved in many pathological processes, both in immunocompetent and immunocompromised individuals. Their isolation, cultivation and correct identification differs significantly from the workup of aerobic species, although the use of new technologies (e.g., matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, whole genome sequencing) changed anaerobic diagnostics dramatically...
November 7, 2017: Antibiotics
https://www.readbyqxmd.com/read/29102775/blandm-1-producing-multidrug-resistant-escherichia-coli-isolated-from-a-companion-dog-in-china
#7
Lanqing Cui, Lei Lei, Yuan Lv, Rongmin Zhang, Xiaoyu Liu, Mei Li, Fan Zhang, Yang Wang
OBJECTIVES: This sudy aimed to characterize a blaNDM-1-producing Escherichia coli from a companion dog. METHODS: This strain was isolated from a surveillance study of carbapenem-resistant Gram-negative bacteria and the specie was identified by API 20E system and MALDI-TOF MS. MICs of various antimicrobial agents were determined by agar dilution. Multilocus sequence typing (MLST) and various carbapenemase genes including blaNDM, blaIMP, blaVIM, blaSME, blaIMI, blaKPC, blaGES, blaSPM, blaGIM, blaNMC, blaDIM, blaSIM, blaOXA-23, blaOXA-24, blaOXA-48, blaOXA-51 and blaOXA-58 were screened by PCR...
November 1, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29101081/molecular-characterization-of-multidrug-resistant-bacteroides-isolates-from-hungarian-clinical-samples
#8
Károly Péter Sárvári, József Sóki, Katalin Kristóf, Emese Juhász, Cecilia Miszti, Szilvia Zsóka Melegh, Krisztina Latkóczy, Edit Urbán
INTRODUCTION: Members of the Bacteroides fragilis group are the most important components of the normal human gut microbiota, but these bacteria can cause severe infections as well. Due to the frequent usage of antibiotics, the spreading of the MDR strains is a real threat worldwide. MATERIALS AND METHODS: In a multicentre study 400 Bacteroides isolates from five Hungarian microbiological laboratories were cultured and identified by MALDI TOF-MS. The MIC values of ten antibiotics were determined by the agar dilution method and evaluated by the breakpoints of EUCAST or CLSI...
October 31, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29079617/staphylococcus-edaphicus-sp-nov-isolated-in-antarctica-harbours-mecc-gene-and-genomic-islands-with-suspected-role-in-adaptation-to-extreme-environment
#9
Roman Pantůček, Ivo Sedláček, Adéla Indráková, Veronika Vrbovská, Ivana Mašlaňová, Vojtěch Kovařovic, Pavel Švec, Stanislava Králová, Lucie Krištofová, Jana Kekláková, Petr Petráš, Jiří Doškař
Two Gram-stain-positive, coagulase-negative staphylococcal strains were isolated from abiotic sources, stone fragments and sandy soil in James Ross Island, Antarctica. Here we describe properties of novel species of the genus Staphylococcus that has near identical 16S rRNA gene sequence to Staphylococcus saprophyticus However, compared to S. saprophyticus and the next closest relatives, the new species demonstrates considerable phylogenetic distance at whole genome level, average nucleotide identity <85 %, and inferred DNA-DNA hybridization <30%...
October 27, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29079147/rapid-detection-of-antibiotic-resistance-by-maldi-tof-mass-spectrometry-using-a-novel-direct-on-target-microdroplet-growth-assay
#10
E A Idelevich, K Sparbier, M Kostrzewa, K Becker
OBJECTIVES: We aimed to develop a universal phenotypic method, which allows easy and rapid antimicrobial susceptibility testing independently of underlying resistance mechanisms. METHODS: We established a novel direct-on-target microdroplet growth assay for the detection of antibiotic resistance within a few hours, which is based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The microorganisms were incubated with and without meropenem in nutrient broth as microdroplets directly on MALDI-TOF MS target...
October 24, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29054663/characterization-of-bacterial-diversity-in-contaminated-groundwater-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#11
Misty S Martin, Inês C Santos, Doug D Carlton, Paula Stigler-Granados, Zacariah L Hildenbrand, Kevin A Schug
Groundwater is a major source for drinking water in the United States, and therefore, its quality and quantity is of extreme importance. One major concern that has emerged is the possible contamination of groundwater due to the unconventional oil and gas extraction activities. As such, the impacts of exogenous contaminants on microbial ecology is an area to be explored to understand what are the chemical and physical conditions that allow the proliferation of pathogenic bacteria and to find alternatives for water treatment by identifying organic-degrading bacteria...
October 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29038283/ctx-m-15-producing-shewanella-sp-clinical-isolate-expressing-oxa-535-a-chromosome-encoded-oxa-48-variant-putative-progenitor-of-the-plasmid-encoded-oxa-436
#12
Agnès B Jousset, Laura Dabos, Rémy A Bonnin, Delphine Girlich, Anaïs Potron, Nicolas Cabanel, Laurent Dortet, Philippe Glaser, Thierry Naas
Shewanella spp. constitute a reservoir of antibiotic resistance determinants. In a bile sample, we have identified three Extended Spectrum β-lactamase (ESBL)-producing bacteria (Escherichia coli, Klebsiella pneumoniae and Shewanella sp. JAB-1) isolated from a child suffering from cholangitis. Our objectives were to characterize the genome and the resistome of the first ESBL-producing isolate of the genus Shewanella and determine whether plasmidic exchange occurred between the three-bacterial species. Bacterial isolates were characterized using MALDI-TOF, standard biochemical tools and antimicrobial susceptibility testing...
October 16, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29027580/screening-for-amyloid-proteins-in-the-yeast-proteome
#13
Tatyana A Ryzhova, Julia V Sopova, Sergey P Zadorsky, Vera A Siniukova, Aleksandra V Sergeeva, Svetlana A Galkina, Anton A Nizhnikov, Aleksandr A Shenfeld, Kirill V Volkov, Alexey P Galkin
The search for novel pathological and functional amyloids represents one of the most important tasks of contemporary biomedicine. Formation of pathological amyloid fibrils in the aging brain causes incurable neurodegenerative disorders such as Alzheimer's, Parkinson's Huntington's diseases. At the same time, a set of amyloids regulates vital processes in archaea, prokaryotes and eukaryotes. Our knowledge of the prevalence and biological significance of amyloids is limited due to the lack of universal methods for their identification...
October 11, 2017: Current Genetics
https://www.readbyqxmd.com/read/28929961/-national-antimicrobial-resistance-surveillance-system-namrss-external-quality-assessment-studies-2011-2016
#14
Serap Süzük Yıldız, Hüsniye Şimşek, Nilay Çöplü, Zeynep Gülay
Establishment of sustainable and evidence-based surveillance systems are recommended for prevention of microbial resistance by the World Health Organization (WHO). As a necessity of these surveillance systems, participants are recommended to implement an external quality assessment (EQA) program. In this scope, National Antimicrobial Resistance Surveillance System (NARSS) has been established within the Public Health Institute of Turkey (PHIT) in our country since 2011. In the scope of this surveillance, NARSS EQA program has been implemented in a cycle per year and four isolates were sent to participants per cycle every year since 2011...
July 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28903168/-sharing-bacterial-microbiota-between-owners-and-their-pets-dogs-cats
#15
Jan Wipler, Zuzana Čermáková, Tomáš Hanzálek, Hana Horáková, Helena Žemličková
INTRODUCTION: The microbiological aspect of a relationship between pets (dogs/cats) and their owners is mainly concerned with the incidence of shared bacterial species, in particular potential pathogens. Given the great popularity of sharing homes with pets (dogs/cats) in the Czech Republic, there is an increased possibility of communication between microbiota of the two macroorganisms (pet and owner). The aim of the study was to determine the biodiversity of shared bacteria and possibility of exchange of genes of resistance to antimicrobial agents between potential pathogens based on the close relationship between pets and humans...
June 2017: Klinická Mikrobiologie a Infekc̆ní Lékar̆ství
https://www.readbyqxmd.com/read/28757016/characterization-of-corynebacterium-diphtheriae-c-confusum-and-c-amycolatum-isolated-from-sows-with-genitourinary-infection
#16
André P Poor, Luisa Z Moreno, Carlos E C Matajira, Beatriz M Parra, Vasco T M Gomes, Ana Paula S Silva, Mauricio C Dutra, Ana Paula G Christ, Mikaela R F Barbosa, Maria Inês Z Sato, Andrea M Moreno
Porcine Corynebacterium infection is still poorly studied, even though the pig has been described as an asymptomatic carrier of Corynebacterium species, including the zoonotic species C. ulcerans, C. confusum and C. amycolatum. Here we present the identification, molecular and antimicrobial susceptibility characterization of coryneform bacteria isolated from sows with urinary tract infection. C. diphtheriae, C. confusum and C. amycolatum were isolated from sows with urinary infection and metritis. Corynebacterium species were identified by MALDI-TOF MS and confirmed by 16S rRNA and rpoB sequencing...
August 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28704596/silver-nanoparticles-functionalized-with-ampicillin
#17
Agnieszka Rogowska, Katarzyna Rafińska, Paweł Pomastowski, Justyna Walczak, Viorica Railean-Plugaru, Magdalena Buszewska-Forajta, Bogusław Buszewski
The resistance of pathogenic bacteria to antibiotics has become a serious problem. The emphasis is placed on the development of new, effective antimicrobial strategies. One of them is the use of AgNPs in association with antibiotic drugs. The aim of this study was to obtain silver nanoparticles functionalized with ampicillin and to investigate the mechanism of binding antibiotics to nanoparticle using high-performance liquid chromatography approach. To confirm the occurrence of silver nanoparticles functionalization, FTIR, MALDI-TOF MS, and DLS analysis and zeta potential measurements were performed...
November 2017: Electrophoresis
https://www.readbyqxmd.com/read/28592862/identification-of-unique-cardiolipin-and-monolysocardiolipin-species-in-acinetobacter-baumannii
#18
Patrizia Lopalco, Julia Stahl, Cosimo Annese, Beate Averhoff, Angela Corcelli
Acidic glycerophospholipids play an important role in determining the resistance of Gram-negative bacteria to stress conditions and antibiotics. Acinetobacter baumannii, an opportunistic human pathogen which is responsible for an increasing number of nosocomial infections, exhibits broad antibiotic resistances. Here lipids of A. baumannii have been analyzed by combined MALDI-TOF/MS and TLC analyses; in addition GC-MS analyses of fatty acid methyl esters released by methanolysis of membrane phospholipids have been performed...
June 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28584333/bacteria-contamination-of-touch-surfaces-in-polish-hospital-wards
#19
Anna Różańska, Dorota Romaniszyn, Agnieszka Chmielarczyk, Małgorzata Bulanda
BACKGROUND: The objective of the study has been to evaluate the pathogenic bacteria contamination of touch surfaces in hospital wards. MATERIAL AND METHODS: Samples were taken from frequently touched surfaces in the hospital environment in 13 units of various types. Culturing was carried out on solid blood agar and in growth broth (tryptic soy broth - TSB). Species identification was performed using the analytical profile index (API) biochemical testing and confirmed with matrix assisted laser desorption ionization time-flight mass spectrometry (MALDI-TOF-MS) system...
June 27, 2017: Medycyna Pracy
https://www.readbyqxmd.com/read/28487432/deciphering-mcr-2-colistin-resistance
#20
Jian Sun, Yongchang Xu, Rongsui Gao, Jingxia Lin, Wenhui Wei, Swaminath Srinivas, Defeng Li, Run-Shi Yang, Xing-Ping Li, Xiao-Ping Liao, Ya-Hong Liu, Youjun Feng
Antibiotic resistance is a prevalent problem in public health worldwide. In general, the carbapenem β-lactam antibiotics are considered a final resort against lethal infections by multidrug-resistant bacteria. Colistin is a cationic polypeptide antibiotic and acts as the last line of defense for treatment of carbapenem-resistant bacteria. Very recently, a new plasmid-borne colistin resistance gene, mcr-2, was revealed soon after the discovery of the paradigm gene mcr-1, which has disseminated globally. However, the molecular mechanisms for MCR-2 colistin resistance are poorly understood...
May 9, 2017: MBio
keyword
keyword
50537
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"