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Maldi bacteria resistance

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https://www.readbyqxmd.com/read/28433670/evaluation-of-the-routine-antimicrobial-susceptibility-testing-results-of-clinically-significant-anaerobic-bacteria-in-a-slovenian-tertiary-care-hospital-in-2015
#1
Samo Jeverica, Urša Kolenc, Manica Mueller-Premru, Lea Papst
The aim of our study was to determined antimicrobial susceptibility profiles of 2673 clinically significant anaerobic bacteria belonging to the major genera, isolated in 2015 in a large tertiary-care hospital in Slovenia. The species identification was performed by MALDI-TOF mass spectrometry. Antimicrobial susceptibility was determined immediately at the isolation of the strains against: penicillin, co-amoxiclav, imipenem, clindamycin and metronidazole, using gradient diffusion methodology and EUCAST breakpoints...
April 19, 2017: Anaerobe
https://www.readbyqxmd.com/read/28418253/species-level-discrimination-of-psychrotrophic-pathogenic-and-spoilage-gram-negative-raw-milk-isolates-using-a-combined-maldi-tof-ms-proteomics-bioinformatics-based-approach
#2
Nuwan R Vithanage, Thomas Richard Yeager, Jeevana Bhongir, Snehal R Jadhav, Chaminda Senaka Ranadheera, Enzo A Palombo, Nivedita Datta
Identification of psychrotrophic pathogenic and spoilage Gram-negative bacteria using rapid and reliable techniques is important in commercial milk processing, as these bacteria can produce heat-resistant proteases and act as post-processing contaminants in pasteurised milk. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) is a proven technology for identification of bacteria in food, however may require optimisation for identification of pathogenic and spoilage bacteria in milk and dairy products...
April 18, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28408060/bacterial-epidemiology-and-antimicrobial-resistance-profiles-of-urinary-specimens-of-the-elderly
#3
F Gravey, G Loggia, A de La Blanchardière, V Cattoir
OBJECTIVE: Although urinary tract infections are the second leading cause of infections among patients aged above 65 years, data on bacterial epidemiology of urinary specimens in these patients is scarce. Our aim was to describe the main bacterial species found at significant levels in urine specimens of the elderly and to determine their antimicrobial resistance profiles. METHODS: From October 2012 to October 2015, all urinary specimens (catheter-related or not) received at the laboratory of microbiology of the university hospital of Caen (France) were retrospectively studied...
April 10, 2017: Médecine et Maladies Infectieuses
https://www.readbyqxmd.com/read/28400760/aeromonas-diversity-and-antimicrobial-susceptibility-in-freshwater-an-attempt-to-set-generic-epidemiological-cut-off-values
#4
Sandrine Baron, Sophie A Granier, Emeline Larvor, Eric Jouy, Maelan Cineux, Amandine Wilhelm, Benoit Gassilloud, Sophie Le Bouquin, Isabelle Kempf, Claire Chauvin
The importance of the role of environment in the dissemination of antimicrobial resistant bacteria is now well recognized. Thus, bacterial indicators to monitor the phenomena are required. The Aeromonas genus is autochthonous in the aquatic environment and easy to detect in any water type, such as freshwater, or wastewater. These microorganisms are also causing infections in humans and animals (including fish). Furthermore, as Aeromonas spp. is able to acquire antimicrobial resistance mechanisms, it is candidate for indicator bacteria to follow antimicrobial resistance dissemination in aquatic environments...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28384185/evaluation-of-a-modified-meropenem-hydrolysis-assay-on-a-large-cohort-of-kpc-and-vim-carbapenemase-producing-enterobacteriaceae
#5
Adriana Calderaro, Mirko Buttrini, Maddalena Piergianni, Sara Montecchini, Monica Martinelli, Silvia Covan, Giovanna Piccolo, Maria Cristina Medici, Maria Cristina Arcangeletti, Carlo Chezzi, Flora De Conto
Carbapenem-resistant Enterobacteriaceae (CRE) have spread globally and represent a serious and growing threat to public health. The introduction of rapid and sensitive methods for the detection of carbapenemase-producing bacteria is of increasing importance. The carbapenemase production can be detected using non-molecular methods (such as the modified Hodge test, the synergy test, the Carba NP test and the antibiotic hydrolysis assays) and DNA-based methods. In this study, we propose a modified version of a previously described meropenem hydrolysis assay (MHA) by MALDI-TOF MS for the phenotypic detection in 2h of carbapenemase-producing Enterobacteriaceae...
2017: PloS One
https://www.readbyqxmd.com/read/28342095/-advances-in-diagnostic-microbiology-opportunities-and-limitations
#6
F P Maurer, M Hentschke, H Rohde
In the light of ever increasing problems related to the emergence of multidrug-resistant bacteria, rapid microbiological diagnostics are of growing importance. Timely pathogen detection and availability of susceptibility data are essential for optimal treatment, but are even more crucial for de-escalation of broad spectrum empiric therapies. Medical microbiology is, thus, an integral part of antimicrobial stewardship programs. Traditional microbiological techniques for species identification and susceptibility testing rely on bacterial growth and are, thus, characterized by inherent slowness...
March 24, 2017: Medizinische Klinik, Intensivmedizin und Notfallmedizin
https://www.readbyqxmd.com/read/28294933/differences-in-sheep-and-goats-milk-microbiological-profile-between-conventional-and-organic-farming-systems-in-greece
#7
Eleni Malissiova, Theofilos Papadopoulos, Aikaterini Kyriazi, Maria Mparda, Christina Sakorafa, Antonios Katsioulis, Anna Katsiaflaka, Maria Kyritsi, Antonios Zdragas, Christos Hadjichristodoulou
The aim of this study was to examine differences in the microbiological profile and antimicrobial resistance of bacteria isolated from milk from organic and conventional sheep and goat farms. Twenty-five organic and 25 conventional sheep and goat farms in the region of Thessaly, Greece participated in this study. A standardised detailed questionnaire was used to describe farming practices. A total of 50 samples were collected and analysed for total viable count (TVC), total coliform count (TCC) and somatic cell count (SCC), while Staphylococcus aureus and Escherichia coli were isolated using standard methods...
March 15, 2017: Journal of Dairy Research
https://www.readbyqxmd.com/read/28284599/the-oral-microbiota-of-domestic-cats-harbors-a-wide-variety-of-staphylococcus-species-with-zoonotic-potential
#8
Ciro César Rossi, Ingrid da Silva Dias, Igor Mansur Muniz, Walter Lilenbaum, Marcia Giambiagi-deMarval
This study aimed to characterize the species, antimicrobial resistance and dispersion of CRISPR systems in staphylococci isolated from the oropharynx of domestic cats in Brazil. Staphylococcus strains (n=75) were identified by MALDI-TOF and sequencing of rpoB and tuf genes. Antimicrobial susceptibility was assessed by disk diffusion method and PCR to investigate the presence of antimicrobial-resistance genes usually present in mobile genetic elements (plasmids), in addition to plasmid extraction. CRISPR - genetic arrangements that give the bacteria the ability to resist the entry of exogenous DNA - were investigated by the presence of the essential protein Cas1 gene...
March 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28283005/-comparison-of-maldi-tof-and-16s-rrna-methods-in-identification-of-viridans-group-streptococci
#9
Serap Süzük Yıldız, Banu Kaşkatepe, Salih Altınok, Mustafa Çetin, Alper Karagöz, Sümeyra Savaş
Accurate identification of viridans group streptococci (VGS) frequently encountered as a causative agent of infective endocarditis is always a challenge for the clinical microbiology laboratory. Clinical microbiology laboratories generally use semi automatic/full automatic systems, molecular methods and also conventional methods for the identification of these bacteria. There are recent published studies that have used MALDI-TOF (Matrix Assisted Laser Ionization Mass Spectrometry-Time of Flight) systems in the identification of VGS...
January 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28219823/proteomic-analysis-of-a-carbapenem-resistant-klebsiella-pneumoniae-strain-in-response-to-meropenem-stress
#10
Arbab Khan, Divakar Sharma, Mohammad Faheem, Deepa Bisht, Asad U Khan
OBJECTIVES: Antibiotic resistance has become a major problem in treating bacterial infections. The aim of this study was to elucidate the effects of meropenem on a blaKPC-2-harbouring multidrug-resistant clinical strain of Klebsiella pneumoniae through a proteomics approach in order to attain a deeper understanding of bacterial resistance strategies. METHODS: Analysis was performed by two-dimensional gel electrophoresis of whole-cell extracts of bacteria exposed to a sublethal concentration of meropenem compared with the untreated control...
February 20, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28192992/protein-adsorbed-magnetic-nanoparticle-mediated-assay-for-rapid-detection-of-bacterial-antibiotic-resistance
#11
Taku A Cowger, Yaping Yang, David E Rink, Trever Todd, Hongmin Chen, Ye Shen, Yajun Yan, Jin Xie
Antibiotic susceptibility tests have been used for years as a crucial diagnostic tool against antibiotic-resistant bacteria. However, due to a lack of biomarkers specific to resistant types, these approaches are often time-consuming, inaccurate, and inflexible in drug selections. Here, we present a novel susceptibility test method named protein-adsorbed nanoparticle-mediated matrix-assisted laser desorption-ionization mass spectrometry, or PANMS. Briefly, we adsorb five different proteins (β-casein, α-lactalbumin, human serum albumin, fibrinogen, and avidin) onto the surface of Fe3O4...
February 17, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28173787/first-time-identification-of-pandoraea-sputorum-from-a-patient-with-cystic-fibrosis-in-argentina-a-case-report
#12
Pablo F Martina, Mónica Martínez, Guillermo Frada, Florencia Alvarez, Lorena Leguizamón, Claudia Prieto, Carolina Barrias, Marisa Bettiol, Antonio Lagares, Alejandra Bosch, Julián Ferreras, Martha Von Specht
BACKGROUND: Pandoraea species are considered emerging pathogens in the context of cystic fibrosis (CF) and are difficult to identify by conventional biochemical methods. These multidrug resistant bacteria remain poorly understood particularly in terms of natural resistance, mechanisms of acquired resistance and impact on the prognosis of the disease and the lung function. Among them, Pandoraea sputorum has been previously described in few cases of CF patients from Spain, Australia, France and United States, underlining the need of more clinical data for a better knowledge of its pathogenicity...
February 7, 2017: BMC Pulmonary Medicine
https://www.readbyqxmd.com/read/28170113/helicoverpa-zea-gut-associated-bacteria-indirectly-induce-defenses-in-tomato-by-triggering-a-salivary-elicitor-s
#13
Jie Wang, Michelle Peiffer, Kelli Hoover, Cristina Rosa, Rensen Zeng, Gary W Felton
Insect gut-associated microbes modulating plant defenses have been observed in beetles and piercing-sucking insects, but the role of caterpillar-associated bacteria in regulating plant induced defenses has not been adequately examined. We identified bacteria from the regurgitant of field-collected Helicoverpa zea larvae using 16S ribosomal RNA (rRNA) gene sequencing and matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry. A combination of biochemical, molecular, and confocal electron microscopy methods were used to determine the role of caterpillar-associated bacteria in mediating defenses in Solanum lycopersicum (tomato)...
February 7, 2017: New Phytologist
https://www.readbyqxmd.com/read/28134321/rapid-sputum-multiplex-detection-of-the-m-tuberculosis-complex-mtbc-and-resistance-mutations-for-eight-antibiotics-by-nucleotide-maldi-tof-ms
#14
Kang-Yi Su, Bo-Shiun Yan, Hao-Chieh Chiu, Chong-Jen Yu, So-Yi Chang, Ruwen Jou, Jia-Long Liu, Po-Ren Hsueh, Sung-Liang Yu
The increasing incidence of multidrug-resistant (MDR) and extensively drug-resistant (XDR) Mycobacterium tuberculosis (MTB) adds further urgency for rapid and multiplex molecular testing to identify the MTB complex and drug susceptibility directly from sputum for disease control. A nucleotide matrix-assisted-laser-desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based assay was developed to identify MTB (MTBID panel) and 45 chromosomal mutations for resistance to eight antibiotics (MTBDR panel)...
January 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28119478/rapid-direct-detection-of-carbapenemase-producing-enterobacteriaceae-in-clinical-urine-samples-by-maldi-tof-ms-analysis
#15
Marina Oviaño, Cecilia de la Luna Ramírez, Luis Pedro Barbeyto, Germán Bou
OBJECTIVES: Development of an automated MALDI-TOF MS-based method for the rapid, direct detection of carbapenemase-producing Enterobacteriaceae in clinical urine samples within 90 min of sample reception. METHODS: A total of 3041 urine samples were processed by flow cytometry, and a cut-off value of ≥1.5 × 10(5) bacteria/mL was used to select samples for the study. Following these criteria, 608 samples were selected for direct bacterial identification...
January 23, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28108122/-fast-methods-of-fungal-and-bacterial-identification-maldi-tof-mass-spectrometry-chromogenic-media
#16
María Siller-Ruiz, Sara Hernández-Egido, Fernando Sánchez-Juanes, José Manuel González-Buitrago, Juan Luis Muñoz-Bellido
MALDI-TOF mass spectrometry is now a routine resource in Clinical Microbiology, because of its speed and reliability in the identification of microorganisms. Its performance in the identification of bacteria and yeasts is perfectly contrasted. The identification of mycobacteria and moulds is more complex, due to the heterogeneity of spectra within each species. The methodology is somewhat more complex, and expanding the size of species libraries, and the number of spectra of each species, will be crucial to achieve greater efficiency...
January 17, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/28092721/use-of-maldi-tof-mass-spectrometry-in-the-battle-against-bacterial-infectious-diseases-recent-achievements-and-future-perspectives
#17
Vassilios Sandalakis, Ioannis Goniotakis, Iosif Vranakis, Dimosthenis Chochlakis, Anna Psaroulaki
Advancements in microbial identification occur increasingly faster as more laboratories explore, refine and extend the use of mass spectrometry in the field of microbiology. Areas covered: This review covers the latest knowledge found in the literature for quick identification of various classes of bacterial pathogens known to cause human infection by the use of MALDI-TOF MS technology. Except for identification of bacterial strains, more researchers try to 'battle time' in favor of the patient. These novel approaches to identify bacteria directly from clinical samples and even determine antibiotic resistance are extensively revised and discussed...
January 22, 2017: Expert Review of Proteomics
https://www.readbyqxmd.com/read/28018582/carriage-of-esbl-ampc-producing-or-ciprofloxacin-non-susceptible-escherichia-coli-and-klebsiella-spp-in-healthy-people-in-norway
#18
Charlotte R Ulstad, Margrete Solheim, Sophie Berg, Morten Lindbæk, Ulf R Dahle, Astrid L Wester
BACKGROUND: Asymptomatic carriage has been recognised as an important risk factor for infection caused by antibiotic resistant bacteria. A 14% global prevalence of Extended-Spectrum Beta-lactamase (ESBL) carriage was recently reported, but large intra-and interregional variations were observed. We investigated the faecal carriage rates of ESBL-, AmpC-producing and ciprofloxacin non-susceptible Escherichia coli and Klebsiella spp. in healthy Norwegians. METHODS: Rectal samples were obtained from 284 volunteers, together with demographic data and information on recent travel history...
2016: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/27942042/faecal-carriage-of-gram-negative-multidrug-resistant-bacteria-among-patients-hospitalized-in-two-centres-in-ulaanbaatar-mongolia
#19
Bayaraa Baljin, Ganbaatar Baldan, Battogtokh Chimeddorj, Khosbayar Tulgaa, Batbaatar Gunchin, Tsogtsaikhan Sandag, Klaus Pfeffer, Colin R MacKenzie, Andreas F Wendel
Gram-negative multidrug-resistant organisms (GN-MDRO) producing β-lactamases (ESBL, plasmid-mediated AmpC β-lactamases and carbapenemases) are increasingly reported throughout Asia. The aim of this surveillance study was to determine the rate of bacterial colonization in patients from two hospitals in the Mongolian capital Ulaanbaatar. Rectal swabs were obtained from patients referred to the National Traumatology and Orthopaedics Research Centre (NTORC) or the Burn Treatment Centre (BTC) between July and September 2014, on admission and again after 14 days...
2016: PloS One
https://www.readbyqxmd.com/read/27889878/same-day-identification-and-antibiotic-susceptibility-testing-on-positive-blood-cultures-a-simple-and-inexpensive-procedure
#20
K Maelegheer, E Nulens
Fast diagnostic tools are becoming a hot topic in microbiology, especially in the case of septic patients. Therefore, we attempted to develop a fast, inexpensive, accurate and easy method to identify bacteria and perform an antibiotic susceptibility test directly on positive blood cultures that could be used in a routine laboratory. A procedure based on centrifugation and washing steps was performed on 110 non-duplicated (including nine seeded) positive blood culture bottles. Direct identification (DID) and antimicrobial susceptibility testing (AST) was conducted on the pellet with the MALDI Biotyper and Phoenix, respectively...
April 2017: European Journal of Clinical Microbiology & Infectious Diseases
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