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https://www.readbyqxmd.com/read/27889878/same-day-identification-and-antibiotic-susceptibility-testing-on-positive-blood-cultures-a-simple-and-inexpensive-procedure
#1
K Maelegheer, E Nulens
Fast diagnostic tools are becoming a hot topic in microbiology, especially in the case of septic patients. Therefore, we attempted to develop a fast, inexpensive, accurate and easy method to identify bacteria and perform an antibiotic susceptibility test directly on positive blood cultures that could be used in a routine laboratory. A procedure based on centrifugation and washing steps was performed on 110 non-duplicated (including nine seeded) positive blood culture bottles. Direct identification (DID) and antimicrobial susceptibility testing (AST) was conducted on the pellet with the MALDI Biotyper and Phoenix, respectively...
November 26, 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27888481/rapid-bacteria-identification-from-environmental-mining-samples-using-maldi-tof-ms-analysis
#2
Ingrid Regina Avanzi, Louise Hase Gracioso, Marcela Dos Passos Galluzzi Baltazar, Bruno Karolski, Elen Aquino Perpetuo, Claudio Augusto Oller do Nascimento
Copper mining has polluted soils and water, causing a reduction of the microbial diversity and a change in the structure of the resident bacterial communities. In this work, selective isolation combined with MALDI-TOF MS and the 16S rDNA method were used for characterizing cultivable bacterial communities from copper mining samples. The results revealed that MALDI-TOF MS analysis can be considered a reliable and fast tool for identifying copper-resistant bacteria from environmental samples at the genera level...
November 25, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27866257/detection-of-methicillin-resistant-staphylococcus-aureus-using-phage-amplification-combined-with-matrix-assisted-laser-desorption-ionization-mass-spectrometry
#3
Jon C Rees, John R Barr
Antibiotic resistance continues to contribute significantly to morbidity and mortality across the world. Developing new tests for antibiotic-resistant bacteria is a core action to combat resistant infections. We describe a method that uses phage amplification detection (PAD) combined with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) to rapidly identify Staphylococcus aureus and determine phenotypic susceptibility to cefoxitin. Samples tested for S. aureus are incubated together with bacteriophage in the presence and absence of cefoxitin and subjected to rapid trypsin digestion followed by MALDI-MS analysis...
November 19, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27836381/universal-protocol-for-the-rapid-automated-detection-of-carbapenem-resistant-gram-negative-bacilli-directly-from-blood-cultures-by-matrix-assisted-laser-desorption-ionisation-time-of-flight-mass-spectrometry-maldi-tof-ms
#4
Marina Oviaño, Katrin Sparbier, Maria José Barba, Markus Kostrzewa, Germán Bou
Detection of carbapenemase-producing bacteria directly from blood cultures is a major challenge, as patients with bacteraemia are critically ill. Early detection can be helpful for selection of the most appropriate antibiotic therapy as well as adequate control of outbreaks. In the current study, a novel matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF)-based method was developed for the rapid, automated detection of carbapenemase-producing Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter baumannii directly from blood cultures...
October 20, 2016: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/27826358/use-of-molecular-typing-to-investigate-bacterial-translocation-from-the-intestinal-tract-of-chlorpyrifos-exposed-rats
#5
Claire Joly Condette, Bertin Elion Dzon, Farida Hamdad, Maurice Biendo, Véronique Bach, Hafida Khorsi-Cauet
BACKGROUND: Human are confronted on a daily basis with contaminant pesticide residues in food, water and other components of the environment. Although the digestive system is the first organ to come into contact with food contaminants, very few data are available on the impact of low-dose pesticide exposure during the in utero and postnatal periods on intestinal bacterial translocation (BT). Previous studies have revealed that chlorpyrifos (CPF) exposure is associated with intestinal dysbiosis and the contamination of sterile organs...
2016: Gut Pathogens
https://www.readbyqxmd.com/read/27794265/molecular-epidemiology-of-environmental-and-clinical-carbapenemase-producing-gram-negative-bacilli-from-hospitals-in-guelma-algeria-multiple-genetic-lineages-and-first-report-of-oxa-48-in-enterobacter-cloacae
#6
Widad Bouguenoun, Sofiane Bakour, Ahmed Aimen Bentorki, Charbel Al Bayssari, Tarek Merad, Jean-Marc Rolain
This study was designed to investigate environmental colonisation in Algerian hospitals by carbapenem-resistant Gram-negative bacilli (GNB), including molecular characterisation of their resistance, and to perform a comparative molecular analysis between clinical and environmental strains. GNB isolated from hospitalised patients and the hospital environment were identified using microbiological methods and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS). Antibiotic susceptibility testing was performed by disk diffusion and Etest methods...
December 2016: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/27790857/a-self-immobilizing-and-fluorogenic-probe-for-%C3%AE-lactamases-detection
#7
Wuyu Mao, Lingying Xia, Yaqun Wang, Hexin Xie
The spread of antibiotic resistance in pathogenic bacteria has become one of the major concerns to public health. Improved monitoring of drug resistance is of high importance for disease control. One of the major mechanisms for bacteria to overcome treatment of antibiotics is the production of antibiotic-hydrolysis enzymes named as β-lactamases. In this study, we have developed a self-immobilizing and fluorogenic probe for the detection of β-lactamase activities. This fluorogenic reagent, upon activation by β-lactamases, turns on fluorescence signal and, more importantly, generates a covalent linkage to the target enzymes and the nearby proteins...
October 28, 2016: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/27726502/resistance-in-bacteria-and-indirect-beta-lactamase-detection-in-e-coli-isolated-from-culex-pipiens-detected-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#8
Lukáš Hleba, Vladimír Kmeť, Tomáš Tóth, Miroslava Kačániová
The aim of this study was detections of antibiotic resistance and resistance mechanism in bacteria isolated from mosquitos (Culex pipiens) living near humans. Therefore, antibiotic resistance in bacteria isolated from Culex pipiens was investigated by disk diffusion test and MIC E-test in this study. MALDI-TOF mass spectrometry was used for detection of resistant mechanism. In this study, hydrolytic breakdown products after a few hours of incubation of the bacteria isolated from Culex pipiens were detected...
October 11, 2016: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/27707676/erysipelothrix-rhusiopathiae-bacteremia-a-challenging-diagnosis
#9
Maïté Micaelo, Pascal Rasmy, Marlène Amara, Juliette Lambert, Aymeric Coutard, Béatrice Pangon
Erysipelothrix rhusiopathiae, a Gram-positive bacillus, is reported to cause for cutaneous infections and endocarditis. We report a case of E. rhusiopathiae bacteremia without severe clinical illness. The patient, a 74-year-old man, is suffering from a chronic lymphoid leukemia (LLC). Following a trauma, the patient developed a bruise on the left inch. Because the site of shock seemed clinically infected, oral amoxicilline-acid clavulanic (AAC) treatment was started after withdrawn 1 set of blood cultures. These blood culture specimens yielded a Gram-positive bacillus identified as E...
October 1, 2016: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/27703855/accuracy-of-conventional-identification-methods-used-for-enterobacteriaceae-isolates-in-three-nigerian-hospitals
#10
Christiana Jesumirhewe, Peter Oladejo Ogunlowo, Mitsan Olley, Burkhard Springer, Franz Allerberger, Werner Ruppitsch
BACKGROUND: Enterobacteriaceae are ubiquitously present in nature and can be found in the intestinal tract of humans and animals as commensal flora. Multidrug-resistant Enterobacteriaceae are increasingly reported and are a threat to public health implicating a need for accurate identification of the isolates to species level. In developing countries, identification of bacteria basically depends on conventional methods: culture and phenotypic methods that hamper the accurate identification of bacteria...
2016: PeerJ
https://www.readbyqxmd.com/read/27688001/evaluation-of-bacteriocinogenic-activity-safety-traits-and-biotechnological-potential-of-fecal-lactic-acid-bacteria-lab-isolated-from-griffon-vultures-gyps-fulvus-subsp-fulvus
#11
Sara Arbulu, Juan J Jiménez, Loreto Gútiez, Cristina Campanero, Rosa Del Campo, Luis M Cintas, Carmen Herranz, Pablo E Hernández
BACKGROUND: Lactic acid bacteria (LAB) are part of the gut microbiota and produce ribosomally synthesized antimicrobial peptides or bacteriocins with interest as natural food preservatives and therapeutic agents. Bacteriocin-producing LAB are also attractive as probiotics. Griffon vultures (Gyps fulvus subspecies fulvus) are scavenger birds that feed almost exclusively on carrion without suffering apparent ill effects. Therefore, griffon vultures might be considered a reservoir of bacteriocin-producing lactic acid bacteria (LAB) with potential biotechnological applications...
September 29, 2016: BMC Microbiology
https://www.readbyqxmd.com/read/27676238/oxidative-dna-damage-and-repair-in-the-radioresistant-archaeon-thermococcus-gammatolerans
#12
Ewa Barbier, Arnaud Lagorce, Amine Hachemi, Murielle Dutertre, Aurore Gorlas, Lucie Morand, Christine Saint-Pierre, Jean-Luc Ravanat, Thierry Douki, Jean Armengaud, Didier Gasparutto, Fabrice Confalonieri, Jean Breton
The hyperthermophilic archaeon Thermococcus gammatolerans can resist huge doses of γ-irradiation, up to 5.0 kGy, without loss of viability. The potential to withstand such harsh conditions is probably due to complementary passive and active mechanisms, including repair of damaged chromosomes. In this work, we documented the formation and repair of oxidative DNA lesions in T. gammatolerans. The basal level of the oxidized nucleoside, 8-oxo-2'-deoxyguanosine (8-oxo-dGuo), was established at 9.2 (± 0.9) 8-oxo-dGuo per 10(6) nucleosides, a higher level than those usually measured in eukaryotic cells or bacteria...
October 12, 2016: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/27672388/uv-b-radiation-stress-causes-alterations-in-whole-cell-protein-profile-and-expression-of-certain-genes-in-the-rice-phyllospheric-bacterium-enterobacter-cloacae
#13
Jay Kumar, Piyoosh K Babele, Divya Singh, Ashok Kumar
Among the different types of UV radiation, UV-B radiation (280-315 nm) has gained much attention mainly due to its increasing incidence on the Earth's surface leading to imbalances in natural ecosystems. This study deals with the effects of UV-B radiation on the proteome and gene expression in a rice phyllospheric bacterium, Enterobacter cloacae. Of the five bacteria isolated from rice leaves, E. cloacae showed the highest level of resistance to UV-B and total killing occurred after 8 h of continuous exposure to UV-B...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27629893/evaluation-of-a-semi-quantitative-maldi-tof-ms-method-for-rapid-antimicrobial-susceptibility-testing-of-positive-blood-cultures
#14
Jette S Jung, Christina Hamacher, Birgit Gross, Katrin Sparbier, Christoph Lange, Markus Kostrzewa, Sören Schubert
With the increasing prevalence of multidrug-resistant Gram-negative bacteria rapid identification of the pathogen and its individual antibiotic resistance is crucial to ensure adequate anti-infective treatment at the earliest time point. MALDI-TOF mass spectrometry for the identification of bacteria directly from the blood culture bottle has been widely established; however, there is still an urgent need for new methods that allow for rapid resistance testing. Recently, a semi-quantitative MALDI-TOF mass spectrometry-based method for the prediction of antibiotic resistance has been described...
September 14, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27602434/sensitivity-to-enterocins-of-thermophilic-campylobacter-spp-from-different-poultry-species
#15
Jana Ščerbová, Andrea Lauková
Campylobacter spp. have been isolated from various animals, including poultry. They are rapidly transmitted throughout broiler sheds by the fecal-oral route. A promising strategy to reduce Campylobacter spp. in poultry may be done due to the beneficial properties of probiotic bacteria and their bacteriocins. In this study, inhibition spectrum/activity of different enterocins was evaluated against Campylobacter spp. (isolated from different poultry) to indicate further practical use of enterocins. Enterocins are antimicrobial proteinaceous substances produced mostly by enterococci...
September 7, 2016: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/27598775/low-enteric-colonization-with-multidrug-resistant-pathogens-in-soldiers-returning-from-deployments-experience-from-the-years-2007-2015
#16
Hagen Frickmann, Dorothea Wiemer, Claudia Frey, Ralf Matthias Hagen, Rebecca Hinz, Andreas Podbielski, Thomas Köller, Philipp Warnke
This assessment describes the enteric colonization of German soldiers 8-12 weeks after returning from mostly but not exclusively subtropical or tropical deployment sites with third-generation cephalosporin-resistant Enterobacteriaceae, vancomycin-resistant enterococci (VRE), and methicillin-resistant Staphylococcus aureus (MRSA). Between 2007 and 2015, 828 stool samples from returning soldiers were enriched in nonselective broth and incubated on selective agars for Enterobacteriaceae expressing extended-spectrum beta-lactamases (ESBL), VRE and MRSA...
2016: PloS One
https://www.readbyqxmd.com/read/27552433/bacterial-diversity-and-antibiotic-resistances-of-abundant-aerobic-culturable-bacteria-in-input-and-output-samples-of-15-german-biogas-plants
#17
T Schauss, T K Wings, J S Brunner, S P Glaeser, W Dott, P Kämpfer
AIM: Determination of the diversities and antibiotic resistances of the most abundant aerobic culturable bacteria and their survival in 15 German biogas plants (BGPs). METHODS AND RESULTS: Microbiological standard media (complex and selective media) were applied to enumerate mesophilic and oligotrophic bacteria (Enterobacteriaceae, Salmonella, staphylococci and enterococci) in input and output samples of BGPs. Concentrations of aerobic culturable bacteria in outputs were mostly one to two orders of magnitude lower than in input samples...
December 2016: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/27525402/-a-sepsis-case-caused-by-a-rare-opportunistic-pathogen-bacillus-pumilus
#18
Barış Ata Borsa, Mehmet Ersoy Aldağ, Birsen Tunalı, Uğur Dinç, Zeynep Güngördü Dalar, Veli Cengiz Özalp
The high prevalence of Bacillus species in nature and the detection of these bacteria as contaminant in cultures may lead diagnostic dilemma, however they should still be considered as a pathogen particularly in case of repeated positive cultures from patients with risk factors. Bacillus pumilus is a bacteria, though rarely, been reported as the causative agent of various infections such as sepsis, endocarditis, skin infections and food poisoning in human. In this report, a sepsis case in an immunocompetent patient caused by B...
July 2016: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/27487518/escherichia-coli-strains-from-ostriches-and-their-sensitivity-to-antimicrobial-substances
#19
J Ščerbová, A Lauková
Ostriches are bred especially for their high-quality meat. There is a lack of knowledge concerning the ostrich's microflora. Escherichia coli is a commensal microorganism of the poultry intestine, ostriches included. However, some strains may become pathogenic. This study was therefore undertaken to detect coliform bacteria in ostrich faeces and to test their antibiotic profile and sensitivity to enterocins. Faeces (n=54, 18 mixture samples from 3 different age groups of 140 ostriches) were sampled to isolate coliform bacteria...
2016: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/27389294/-future-applications-of-mass-spectrometry-in-microbiology
#20
Jordi Vila, Yuliya Zboromyrska, Almudena Burillo, Emilio Bouza
MALDI-TOF (matrix-assisted laser desorption ionization time-of-flight) mass spectrometry (MS) has been vigorously introduced in many clinical microbiology laboratories for the rapid and accurate identification of bacteria and fungi. In fact, the implementation of this methodology can be considered a revolution in these laboratories. In addition to microbial identification, MALDI-TOF MS is being used for the detection of some mechanisms of antibiotic resistance and for the molecular typing of bacteria. A number of current and future applications that increase the versatility of this methodology may also be mentioned...
June 2016: Enfermedades Infecciosas y Microbiología Clínica
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