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Maldi bacteria resistance

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https://www.readbyqxmd.com/read/29608117/clinical-impact-of-rapid-bacterial-identification-by-maldi-tof-ms-combined-with-the-b%C3%A3%C2%AAta-lacta%C3%A2-test-on-early-antibiotic-adaptation-by-an-antimicrobial-stewardship-team-in-bloodstream-infections
#1
A Mizrahi, J Amzalag, C Couzigou, G Péan De Ponfilly, B Pilmis, A Le Monnier
BACKGROUND: Bloodstream infections (BSI) can potentially be life-threatening infections and are associated with a high crude mortality, moreover with an inappropriate first-line antibiotic therapy. Bacterial resistance is more and more frequently observed. New strategies of BSI management are urgently needed. MATERIALS AND METHODS: During an 18-months period, we prospectively evaluated the clinical impact of rapid bacterial identification by MALDI-TOF MS technology combined with an antimicrobial stewardship team (AST) intervention...
April 2, 2018: Infectious Diseases
https://www.readbyqxmd.com/read/29590370/kpc-2-carbapenemase-producing-pseudomonas-aeruginosa-reaching-germany
#2
Jürgen Benjamin Hagemann, Niels Pfennigwerth, Sören G Gatermann, Heike von Baum, Andreas Essig
Background: Antimicrobial resistance due to carbapenemase expression poses a worldwide threat in healthcare. Inter-genus exchange of genetic information is of utmost importance in this context. Objectives: Here, to the best of our knowledge, we describe the first detection and characterization of a KPC-2-producing Pseudomonas aeruginosa in Germany. Methods: Characterization of the isolate was performed using MALDI-TOF MS, automated microdilution and MLST...
March 24, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29580515/probiotic-potential-of-yeasts-isolated-from-pineapple-and-their-use-in-the-elaboration-of-potentially-functional-fermented-beverages
#3
Juliana Cunha Amorim, Roberta Hilsdorf Piccoli, Whasley Ferreira Duarte
Pineapple (Ananas comosus (L.) Merril) is a tropical fruit rich in nutrients characterized by a pleasant taste and widely consumed in several countries. It is used to produce juice, jams and wine. In this work, 150 yeasts isolates were obtained from peel (18) and spontaneously fermented pineapple pulp (132). The probiotic potential of 50 isolates was studied. Survival at pH 2.0, pepsin 3.0 g/L, and tolerance of bile salts (0.1 and 1% (w/v) were determined as indicators of survival potential of the isolates during the passage through the human gastrointestinal tract in simulated conditions...
May 2018: Food Research International
https://www.readbyqxmd.com/read/29550436/bioaccumulation-of-cadmium-by-enterobacter-sp-and-enhancement-of-rice-seedling-growth-under-cadmium-stress
#4
Soumik Mitra, Krishnendu Pramanik, Anumita Sarkar, Pallab Kumar Ghosh, Tithi Soren, Tushar Kanti Maiti
Bacteria-mediated plant growth promotion and bioremediation of heavy metal containing soil is a widely accepted eco-friendly method. The present study is aimed to screen out cadmium resistant bacterial strain from metal contaminated rice rhizosphere and evaluate its effects on the growth of rice seedlings under cadmium stress. Among four different isolates (designated as S1, S2, S3 and S5), the S2 isolate was screened on the basis of different PGP traits and multi heavy metal resistance (minimum inhibitory concentration for cadmium, lead and arsenic were 3500, 2500 and 1050 µg/ml respectively)...
March 15, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29534106/a-new-scheme-for-strain-typing-of-methicillin-resistant-staphylococcus-aureus-on-the-basis-of-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-by-using-machine-learning-approach
#5
Hsin-Yao Wang, Tzong-Yi Lee, Yi-Ju Tseng, Tsui-Ping Liu, Kai-Yao Huang, Yung-Ta Chang, Chun-Hsien Chen, Jang-Jih Lu
Methicillin-resistant Staphylococcus aureus (MRSA), one of the most important clinical pathogens, conducts an increasing number of morbidity and mortality in the world. Rapid and accurate strain typing of bacteria would facilitate epidemiological investigation and infection control in near real time. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry is a rapid and cost-effective tool for presumptive strain typing. To develop robust method for strain typing based on MALDI-TOF spectrum, machine learning (ML) is a promising algorithm for the construction of predictive model...
2018: PloS One
https://www.readbyqxmd.com/read/29527202/comprehensive-evaluation-of-the-mbt-star-bl-module-for-simultaneous-bacterial-identification-and-%C3%AE-lactamase-mediated-resistance-detection-in-gram-negative-rods-from-cultured-isolates-and-positive-blood-cultures
#6
Annie W T Lee, Johnson K S Lam, Ricky K W Lam, Wan H Ng, Ella N L Lee, Vicky T Y Lee, Po P Sze, Rahim Rajwani, Kitty S C Fung, Wing K To, Rodney A Lee, Dominic N C Tsang, Gilman K H Siu
Objective: This study evaluated the capability of a MALDI Biotyper system equipped with the newly introduced MBT STAR-BL module to simultaneously perform species identification and β-lactamase-mediated resistance detection in bacteremia -causing bacteria isolated from cultured isolates and patient-derived blood cultures (BCs). Methods: Two hundred retrospective cultured isolates and 153 prospective BCs containing Gram-negative rods (GNR) were collected and subjected to direct bacterial identification, followed by the measurement of β-lactamase activities against ampicillin, piperacillin, cefotaxime, ceftazidime, and meropenem using the MBT STAR-BL module...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29508232/rare-case-of-osteomyelitis-of-tibial-shaft-caused-by-nocardia-cyriacigeorgica
#7
D Raszka, S Popelka, J Heřt, D Jahoda, I Landor, P Vavřík
Nocardiosis is a rare infection caused by the aerobic actinomycete of the Nocardia genus. In most cases, nocardiosis manifests as a lung infection or a bone lesion. Due to the nonspecific and mild clinical manifestations of nocardiosis, the establishment of definite diagnosis can be difficult. When antibiotic therapy is incorrectly targeted, only the symptoms of the disease are suppressed. The mainstay in the treatment of Nocardia osteomyelitis has so far been the combined surgical debridement with long-term, initially intravenous, antibiotic administration...
March 5, 2018: Folia Microbiologica
https://www.readbyqxmd.com/read/29499232/direct-detection-of-carbapenemase-associated-proteins-of-acinetobacter-baumannii-using-nanodiamonds-coupled-with-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#8
Kai-Chih Chang, Chin-Yi Chung, Chen-Hsing Yeh, Kuo-Hsiu Hsu, Ya-Ching Chin, Sin-Siang Huang, Bo-Rong Liu, Hsi-An Chen, Anren Hu, Po-Chi Soo, Wen-Ping Peng
The appearance and spread of carbapenem-resistant Acinetobacter baumannii (CRAB) pose a challenge for optimization of antibiotic therapies and outbreak preventions. The carbapenemase production can be detected through culture-based methods (e.g. Modified Hodge Test-MHT) and DNA based methods (e.g. Polymerase Chain Reaction-PCR). The culture-based methods are time-consuming, whereas those of PCR assays need only a few hours but due to its specificity, can only detect known genetic targets encoding carbapenem-resistance genes...
February 27, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29462787/mechanistic-insights-into-transferable-polymyxin-resistance-among-gut-bacteria
#9
Yongchang Xu, Jingxia Lin, Tao Cui, Swaminath Srinivas, Youjun Feng
Polymyxins such as colistin are antibiotics used as a final line of defense in the management of infections with multidrug-resistant Gram-negative bacteria. Although natural resistance to polymyxins is rare, the discovery of a mobilized colistin resistance gene ( mcr-1 ) in gut bacteria has raised significant concern. As an intramembrane enzyme, MCR-1 catalyzes the transfer of phosphoethanolamine (PEA) to the 1 (or 4')-phosphate group of the lipid A moiety of lipopolysaccharide (LPS), thereby conferring colistin resistance...
February 9, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29411729/fecal-carriage-of-extended-spectrum-beta-lactamase-and-ampc-beta-lactamase-producing-enterobacteriaceae-in-a-turkish-community
#10
G Hazirolan, I Mumcuoglu, G Altan, B B Özmen, N Aksu, Z C Karahan
BACKGROUND: Community-acquired infection caused by extended-spectrum beta-lactamase (ESBL)-producing microorganisms has an increasing frequency. AIM: The aim of this study was to determine the fecal carriage of ESBL and AmpC beta-lactamase-producing Enterobacteriaceae in community and to investigate cefotaxime-M (CTX-M) genes among ESBL isolates. MATERIALS AND METHODS: A total of 1402 fecal specimens which were collected from outpatients included in the study...
January 2018: Nigerian Journal of Clinical Practice
https://www.readbyqxmd.com/read/29325326/-investigation-of-colistin-resistance-gene-mcr-in-gut-bacteria-from-patients-with-acute-diarrheal
#11
Y Li, W J Yan, S Q Liu
Objective: To investigate the presence of colistin resistance gene mcr in gut bacteria from patients with acute diarrhea. Methods: Fresh stool samples were collected from 150 outpatients with acute diarrhea in the intestinal clinic of Henan Provincial People's Hospital, and were directly detected for the presence of mcr by PCR after enrichment in the broth.The mcr-producing bacteria were further isolated and identified by MALDI-TOF MS (Bruker Biotyper). Antimicrobial susceptibility of these isolates was conducted by micro-broth dilution method...
December 19, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29281884/binary-colloidal-crystal-layers-as-platforms-for-surface-patterning-of-puroindoline-based-antimicrobial-peptides
#12
Andrew Boden, Mrinal Bhave, Peng-Yuan Wang, Snehal Jadhav, Peter Kingshott
The ability of bacteria to form biofilms and the emergence of antibiotic-resistant strains have prompted the need to develop the next generation of antibacterial coatings. Antimicrobial peptides (AMPs) are showing promise as molecules that can address these issues, especially if used when immobilized as a surface coating. We present a method that explores how surface patterns together with the selective immobilization of an AMP called PuroA (FPVTWRWWKWWKG-NH2 ) can be used to both kill bacteria and also as a tool to study bacterial attachment mechanisms...
January 24, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29280153/methods-for-identification-and-differentiation-of-different-shewanella-spp-isolates-for-diagnostic-use
#13
V Jung-Schroers, A Jung, M Ryll, J Bauer, F Teitge, D Steinhagen
Shewanella spp. are Gram-negative, rod-shaped, motile bacteria that are widely distributed in marine and freshwater environments. The bacteria are present in the physiological microflora of fish from temperate waters and are known as fish spoilage species. From clinically healthy fish and from fish with skin ulcerations, Shewanella spp. is regularly isolated, indicating a possible role as fish pathogen. In this study, 74 isolates of Shewanella spp. were analysed. For species identification, biochemical techniques, 16S rRNA sequencing, MALDI-TOF MS and the Sherlock Microbial Identification System (MIS) based on the composition of fatty acid ethyl esters were compared...
April 2018: Journal of Fish Diseases
https://www.readbyqxmd.com/read/29279453/evaluation-of-the-filmarray-blood-culture-identification-panel-on-detection-of-pathogenic-microorganisms-in-positive-blood-cultures-the-first-clinical-report-in-japan
#14
Kyoichi Saito, Shiro Endo, Makoto Katsumi, Chiyuki Ishizawa, Yuko Fujikawa, Shinya Inomata, Masahiro Toyokawa, Mitsuo Kaku
FilmArray (FA) is a multiplex PCR-based desktop microbial detection system. The blood culture identification (BCID) panel is an adaptable panel for FA, which diagnoses sepsis and/or systemic infections by detecting 14 bacterial species, 4 bacterial genera, 1 bacterial family, 5 yeast species, and 3 antimicrobial resistance genes (mecA, Klebsiella pneumoniae carbapenemase [KPC], and vanA/B) in positive blood cultures within 1 h. We retrospectively evaluated the FA-BCID panel using 54 positive blood cultures, in which 57 bacterial and 3 yeast strains were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)...
March 22, 2018: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29225047/maldi-tof-ms-analysis-as-a-useful-tool-for-an-identification-of-legionella-pneumophila-a-facultatively-pathogenic-bacterium-interacting-with-free-living-amoebae-a-case-study-from-water-supply-system-of-hospitals-in-bratislava-slovakia
#15
Katarína Trnková, Martina Kotrbancová, Margita Špaleková, Miriam Fulová, Jana Boledovičová, Matej Vesteg
Legionellae, i.e. Legionella pneumophila, are human bacterial hydrophilic facultative pathogens causing pneumonia (Legionnaires' disease). Free-living amoebae (FLA) can serve as natural hosts and thus as reservoirs of many amoebae-resistant bacteria. An encysted amoeba can contribute to the resistance of intracellular L. pneumophila to various chemical and physical treatments. Humans can be infected by droplets containing bacteria from an environmental source or human-made devices such as shower heads, bathtubs, air-conditioning units or whirlpools...
January 2018: Experimental Parasitology
https://www.readbyqxmd.com/read/29203405/inactivation-of-the-arn-operon-and-loss-of-aminoarabinose-on-lipopolysaccharide-as-the-cause-of-susceptibility-to-colistin-in-an-atypical-clinical-isolate-of-proteus-vulgaris
#16
Sophie Baron, Zineb Leulmi, Claude Villard, Abiola Olumuyiwa Olaitan, Amar A Telke, Jean-Marc Rolain
Colistin has become a last-line antibiotic for the treatment of multidrug-resistant bacterial infections; however, resistance to colistin has emerged in recent years. Some bacteria, such as Proteus and Serratia spp., are intrinsically resistant to colistin although the exact mechanism of resistance is unknown. Here we identified the molecular support for intrinsic colistin resistance in Proteus spp. by comparative genomic, transcriptomic and proteomic analyses of colistin-susceptible (CSUR P1868_S) and colistin-resistant (CSUR P1867_R) strains of an atypical Proteus vulgaris...
December 1, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29188069/simultaneous-isolation-of-chryseobacterium-gleum-from-bloodstream-and-respiratory-tract-first-case-report-from-india
#17
Vidhi Jain, Nayani Amrin Fatema Afzal Hussain, Tasneem Siddiqui, Chinmoy Sahu, Malay Ghar, Kashi Nath Prasad
Introduction. Species of the genus Chryseobacterium are emerging healthcare-associated pathogens, often colonizing the hospital environment. There are no clear guidelines available for antimicrobial susceptibility of this organism. In this report we present the first case, to our knowledge, of simultaneous central-line-associated bloodstream infection (CLABSI) and ventilator-associated pneumonia (VAP) due to Chryseobacterium gleum from India. Case presentation. A 62 years old man with a history of a road traffic accident 1 month previously was referred to our center for further management...
October 2017: JMM Case Reports
https://www.readbyqxmd.com/read/29175433/detection-of-ampc-%C3%AE-lactamase-producing-gram-negative-bacteria-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#18
C Li, S Ding, Y Huang, Z Wang, J Shen, H Ling, Y Xu
BACKGROUND: Rapid detection of AmpC-producing strains of Gram-negative bacteria is beneficial for patient care. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a novel method that has demonstrated the resistance of a strain to β-lactam antibiotics by determining the molecular structure of an antibiotic and its degradation products. AIM: To study the detection of AmpC-producing Gram-negative bacteria by MALDI-TOF MS and to determine whether the method can be used in clinical practice...
November 22, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/29146249/composition-of-lactic-acid-bacteria-during-spontaneous-curly-kale-brassica-oleracea-var-sabellica-fermentation
#19
Magdalena Michalak, Klaudia Gustaw, Adam Waśko, Magdalena Polak-Berecka
The present work is the first report on spontaneous fermentation of curly kale and characteristics of autochthonous lactic acid bacteria (LAB). Our results indicate that curly kale fermentation is the new possibility of the technological use of this vegetable. Bacteria representing ten different species were isolated from three phases of curly kale fermentation and identified by MALDI-TOF mass spectrometry and 16S rRNA gene sequencing. Among them, four species were identified as Lactobacillus spp. (Lb. plantarum 332, Lb...
January 2018: Microbiological Research
https://www.readbyqxmd.com/read/29112122/identification-and-antimicrobial-susceptibility-testing-of-anaerobic-bacteria-rubik-s-cube-of-clinical-microbiology
#20
REVIEW
Márió Gajdács, Gabriella Spengler, Edit Urbán
Anaerobic bacteria have pivotal roles in the microbiota of humans and they are significant infectious agents involved in many pathological processes, both in immunocompetent and immunocompromised individuals. Their isolation, cultivation and correct identification differs significantly from the workup of aerobic species, although the use of new technologies (e.g., matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, whole genome sequencing) changed anaerobic diagnostics dramatically...
November 7, 2017: Antibiotics
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