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Maldi bacteria resistance

Lukáš Hleba, Vladimír Kmeť, Tomáš Tóth, Miroslava Kačániová
The aim of this study was detections of antibiotic resistance and resistance mechanism in bacteria isolated from mosquitos (Culex pipiens) living near humans. Therefore, antibiotic resistance in bacteria isolated from Culex pipiens was investigated by disk diffusion test and MIC E-test in this study. MALDI-TOF mass spectrometry was used for detection of resistant mechanism. In this study, hydrolytic breakdown products after a few hours of incubation of the bacteria isolated from Culex pipiens were detected...
October 11, 2016: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
Maïté Micaelo, Pascal Rasmy, Marlène Amara, Juliette Lambert, Aymeric Coutard, Béatrice Pangon
Erysipelothrix rhusiopathiae, a Gram-positive bacillus, is reported to cause for cutaneous infections and endocarditis. We report a case of E. rhusiopathiae bacteremia without severe clinical illness. The patient, a 74-year-old man, is suffering from a chronic lymphoid leukemia (LLC). Following a trauma, the patient developed a bruise on the left inch. Because the site of shock seemed clinically infected, oral amoxicilline-acid clavulanic (AAC) treatment was started after withdrawn 1 set of blood cultures. These blood culture specimens yielded a Gram-positive bacillus identified as E...
October 1, 2016: Annales de Biologie Clinique
Christiana Jesumirhewe, Peter Oladejo Ogunlowo, Mitsan Olley, Burkhard Springer, Franz Allerberger, Werner Ruppitsch
BACKGROUND: Enterobacteriaceae are ubiquitously present in nature and can be found in the intestinal tract of humans and animals as commensal flora. Multidrug-resistant Enterobacteriaceae are increasingly reported and are a threat to public health implicating a need for accurate identification of the isolates to species level. In developing countries, identification of bacteria basically depends on conventional methods: culture and phenotypic methods that hamper the accurate identification of bacteria...
2016: PeerJ
Sara Arbulu, Juan J Jiménez, Loreto Gútiez, Cristina Campanero, Rosa Del Campo, Luis M Cintas, Carmen Herranz, Pablo E Hernández
BACKGROUND: Lactic acid bacteria (LAB) are part of the gut microbiota and produce ribosomally synthesized antimicrobial peptides or bacteriocins with interest as natural food preservatives and therapeutic agents. Bacteriocin-producing LAB are also attractive as probiotics. Griffon vultures (Gyps fulvus subspecies fulvus) are scavenger birds that feed almost exclusively on carrion without suffering apparent ill effects. Therefore, griffon vultures might be considered a reservoir of bacteriocin-producing lactic acid bacteria (LAB) with potential biotechnological applications...
September 29, 2016: BMC Microbiology
Ewa Barbier, Arnaud Lagorce, Amine Hachemi, Murielle Dutertre, Aurore Gorlas, Lucie Morand, Christine Saint-Pierre, Jean-Luc Ravanat, Thierry Douki, Jean Armengaud, Didier Gasparutto, Fabrice Confalonieri, Jean Breton
The hyperthermophilic archaeon Thermococcus gammatolerans can resist huge doses of γ-irradiation, up to 5.0 kGy, without loss of viability. The potential to withstand such harsh conditions is probably due to complementary passive and active mechanisms, including repair of damaged chromosomes. In this work, we documented the formation and repair of oxidative DNA lesions in T. gammatolerans. The basal level of the oxidized nucleoside, 8-oxo-2'-deoxyguanosine (8-oxo-dGuo), was established at 9.2 (± 0.9) 8-oxo-dGuo per 10(6) nucleosides, a higher level than those usually measured in eukaryotic cells or bacteria...
October 12, 2016: Chemical Research in Toxicology
Jay Kumar, Piyoosh K Babele, Divya Singh, Ashok Kumar
Among the different types of UV radiation, UV-B radiation (280-315 nm) has gained much attention mainly due to its increasing incidence on the Earth's surface leading to imbalances in natural ecosystems. This study deals with the effects of UV-B radiation on the proteome and gene expression in a rice phyllospheric bacterium, Enterobacter cloacae. Of the five bacteria isolated from rice leaves, E. cloacae showed the highest level of resistance to UV-B and total killing occurred after 8 h of continuous exposure to UV-B...
2016: Frontiers in Microbiology
Jette S Jung, Christina Hamacher, Birgit Gross, Katrin Sparbier, Christoph Lange, Markus Kostrzewa, Sören Schubert
With the increasing prevalence of multidrug-resistant Gram-negative bacteria rapid identification of the pathogen and its individual antibiotic resistance is crucial to ensure adequate anti-infective treatment at the earliest time point. MALDI-TOF mass spectrometry for the identification of bacteria directly from the blood culture bottle has been widely established; however, there is still an urgent need for new methods that allow for rapid resistance testing. Recently, a semi-quantitative MALDI-TOF mass spectrometry-based method for the prediction of antibiotic resistance has been described...
September 14, 2016: Journal of Clinical Microbiology
Jana Ščerbová, Andrea Lauková
Campylobacter spp. have been isolated from various animals, including poultry. They are rapidly transmitted throughout broiler sheds by the fecal-oral route. A promising strategy to reduce Campylobacter spp. in poultry may be done due to the beneficial properties of probiotic bacteria and their bacteriocins. In this study, inhibition spectrum/activity of different enterocins was evaluated against Campylobacter spp. (isolated from different poultry) to indicate further practical use of enterocins. Enterocins are antimicrobial proteinaceous substances produced mostly by enterococci...
September 7, 2016: Foodborne Pathogens and Disease
Hagen Frickmann, Dorothea Wiemer, Claudia Frey, Ralf Matthias Hagen, Rebecca Hinz, Andreas Podbielski, Thomas Köller, Philipp Warnke
This assessment describes the enteric colonization of German soldiers 8-12 weeks after returning from mostly but not exclusively subtropical or tropical deployment sites with third-generation cephalosporin-resistant Enterobacteriaceae, vancomycin-resistant enterococci (VRE), and methicillin-resistant Staphylococcus aureus (MRSA). Between 2007 and 2015, 828 stool samples from returning soldiers were enriched in nonselective broth and incubated on selective agars for Enterobacteriaceae expressing extended-spectrum beta-lactamases (ESBL), VRE and MRSA...
2016: PloS One
Thorsten Schauss, Tina K Wings, Jana S Brunner, Stefanie P Glaeser, Wolfgang Dott, Peter Kämpfer
AIM: Determination of the diversities and antibiotic resistances of the most abundant aerobic culturable bacteria and their survival in 15 German biogas plants (BGPs). METHODS AND RESULTS: Microbiological standard media (complex and selective media) were applied to enumerate mesophilic and oligotrophic bacteria (Enterobacteriaceae, Salmonella, staphylococci and enterococci) in input and output samples of BGPs. Concentrations of aerobic culturable bacteria in outputs were mostly one to two orders of magnitude lower than in input samples...
August 23, 2016: Journal of Applied Microbiology
Barış Ata Borsa, Mehmet Ersoy Aldağ, Birsen Tunalı, Uğur Dinç, Zeynep Güngördü Dalar, Veli Cengiz Özalp
The high prevalence of Bacillus species in nature and the detection of these bacteria as contaminant in cultures may lead diagnostic dilemma, however they should still be considered as a pathogen particularly in case of repeated positive cultures from patients with risk factors. Bacillus pumilus is a bacteria, though rarely, been reported as the causative agent of various infections such as sepsis, endocarditis, skin infections and food poisoning in human. In this report, a sepsis case in an immunocompetent patient caused by B...
July 2016: Mikrobiyoloji Bülteni
J Ščerbová, A Lauková
Ostriches are bred especially for their high-quality meat. There is a lack of knowledge concerning the ostrich's microflora. Escherichia coli is a commensal microorganism of the poultry intestine, ostriches included. However, some strains may become pathogenic. This study was therefore undertaken to detect coliform bacteria in ostrich faeces and to test their antibiotic profile and sensitivity to enterocins. Faeces (n=54, 18 mixture samples from 3 different age groups of 140 ostriches) were sampled to isolate coliform bacteria...
2016: Polish Journal of Veterinary Sciences
Jordi Vila, Yuliya Zboromyrska, Almudena Burillo, Emilio Bouza
MALDI-TOF (matrix-assisted laser desorption ionization time-of-flight) mass spectrometry (MS) has been vigorously introduced in many clinical microbiology laboratories for the rapid and accurate identification of bacteria and fungi. In fact, the implementation of this methodology can be considered a revolution in these laboratories. In addition to microbial identification, MALDI-TOF MS is being used for the detection of some mechanisms of antibiotic resistance and for the molecular typing of bacteria. A number of current and future applications that increase the versatility of this methodology may also be mentioned...
June 2016: Enfermedades Infecciosas y Microbiología Clínica
Thais Bergamin Lima, Osmar Nascimento Silva, Keyla Caroline de Almeida, Suzana Meira Ribeiro, Dielle de Oliveira Motta, Simone Maria-Neto, Michelle Brizolla Lara, Carlos Roberto Souza Filho, Alicia Simalie Ombredane, Celio de Faria Junior, Nadia Skorupa Parachin, Beatriz Simas Magalhães, Octávio Luiz Franco
Enterobacter cloacae is a Gram-negative bacterium associated with high morbidity and mortality in intensive care patients due to its resistance to multiple antibiotics. Currently, therapy against multi-resistant bacteria consists of using colistin, in spite of its toxic effects at higher concentrations. In this context, colistin-resistant E. cloacae strains were challenged with lower levels of colistin combined with other antibiotics to reduce colistin-associated side effects. Colistin-resistant E. cloacae (ATCC 49141) strains were generated by serial propagation in subinhibitory colistin concentrations...
July 6, 2016: Journal of Antibiotics
Anna Kędziora, Eva Krzyżewska, Bartłomiej Dudek, Gabriela Bugla-Płoskońska
The presented study is to analyze the participation of outer membrane proteins of Gram- negative bacteria in sensitivity to silver nanomaterials. The mechanism of interaction of silver with the bacterial cell is best described in this group of microorganisms. There are several theories regarding the effectiveness of antimicrobial ions and nanosilver, and at the indicated differences in the way they work. Outer membrane proteins of Gram-negative bacteria are involved in the procurement of silver from the environment and contribute to the development mechanisms of resistance to nanometals...
2016: Postȩpy Higieny i Medycyny Doświadczalnej
D Stępień-Pyśniak, J Wilczyński, A Marek, A Śmiech, U Kosikowska, T Hauschild
This report suggests a strong association between coagulase-negative Staphylococcus  simulans and endocarditis in broiler chickens of a single flock. Clinical signs included increased mortality and lameness, and some dead chickens were found on their backs. Lesions included cauliflower-like, fibrinous vegetative lesions on the left atrioventricular (AV) valve; cream-coloured, necrotic foci of varying size in the liver, and necrosis of the femoral head. Histopathological examination of the heart revealed multifocal conglomerates of bacterial colonies attached to the valvular endocardium, threads of fibrin, inflammatory cells with the presence of heterophils...
June 22, 2016: Avian Pathology: Journal of the W.V.P.A
Tobias C Olofsson, Éile Butler, Christina Lindholm, Bo Nilson, Per Michanek, Alejandra Vásquez
In the global perspective of antibiotic resistance, it is urgent to find potent topical antibiotics for the use in human and animal infection. Healing of equine wounds, particularly in the limbs, is difficult due to hydrostatic factors and exposure to environmental contaminants, which can lead to heavy bio-burden/biofilm formation and sometimes to infection. Therefore, antibiotics are often prescribed. Recent studies have shown that honeybee-specific lactic acid bacteria (LAB), involved in honey production, and inhibit human wound pathogens...
October 2016: Current Microbiology
Yannick Charretier, Jacques Schrenzel
Developing elaborate techniques for clinical applications can be a complicated process. Whole-cell MALDI-TOF MS revolutionized reliable microorganism identification in clinical microbiology laboratories and is now replacing phenotypic microbial identification. This technique is a generic, accurate, rapid, and cost-effective growth-based method. Antibiotic resistance keeps emerging in environmental and clinical microorganisms, leading to clinical therapeutic challenges, especially for Gram-negative bacteria...
June 17, 2016: Proteomics. Clinical Applications
Sónia Ramos, Nuno Silva, Michel Hébraud, Hugo M Santos, Júlio Dinis Nunes-Miranda, Luís Pinto, José E Pereira, José-Luis Capelo, Patrícia Poeta, Gilberto Igrejas
Understanding global drug resistance demands an integrated vision, focusing on both human and veterinary medicine. Omics technologies offer new vistas to decipher mechanisms of drug resistance in the food chain. For example, Escherichia coli resistance to major antibiotics is increasing whereas multidrug resistance (MDR) strains are now commonly found in humans and animals. Little is known about the structural and metabolic changes in the cell that trigger resistance to antimicrobial agents. Proteomics is an emerging field that is used to advance our knowledge in global health and drug resistance in the food chain...
June 2016: Omics: a Journal of Integrative Biology
Christian Penny, Beau Grothendick, Lin Zhang, Connie M Borror, Duane Barbano, Angela J Cornelius, Brent J Gilpin, Clifton K Fagerquist, William J Zaragoza, Michele T Jay-Russell, Albert J Lastovica, Catherine Ragimbeau, Henry-Michel Cauchie, Todd R Sandrin
MALDI-TOF MS has been utilized as a reliable and rapid tool for microbial fingerprinting at the genus and species levels. Recently, there has been keen interest in using MALDI-TOF MS beyond the genus and species levels to rapidly identify antibiotic resistant strains of bacteria. The purpose of this study was to enhance strain level resolution for Campylobacter jejuni through the optimization of spectrum processing parameters using a series of designed experiments. A collection of 172 strains of C. jejuni were collected from Luxembourg, New Zealand, North America, and South Africa, consisting of four groups of antibiotic resistant isolates...
2016: Frontiers in Microbiology
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