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https://www.readbyqxmd.com/read/29784358/short-probe-based-duplex-specific-nuclease-signal-amplification-strategy-enables-imaging-of-endogenous-micrornas-in-living-cells-with-ultrahigh-specificity
#1
Yingjun Ma, Jun Chen, Danping Chen, Yuzhi Xu, Li Zhang, Zong Dai, Xiaoyong Zou
Specific nucleic acids amplification at a constant and mild temperature is important for imaging assay of endogenous microRNAs (miRNAs) in living cells. Duplex-specific nuclease (DSN) is attractive in one-step isothermal assay of miRNA; however, its intrinsic limitations of low amplification specificity and high reaction temperature greatly restrict the application scope. Herein, we present a short-probe-based DSN signal amplification (spDSNSA) strategy enabling analysis of miRNAs at body temperature with significantly high specificity...
August 15, 2018: Talanta
https://www.readbyqxmd.com/read/29781118/ballistic-tongue-projection-in-a-miniaturized-salamander
#2
Stephen M Deban, Segall V Bloom
Miniaturization of body size is often accompanied by peculiarities in morphology that can have functional consequences. We examined the feeding behavior and morphology of the miniaturized plethodontid salamander Thorius, one of the smallest vertebrates, to determine if its performance and biomechanics differ from those of its larger relatives. High-speed imaging and dynamics analysis of feeding at a range of temperatures show that tongue projection in Thorius macdougalli is ballistic and achieves accelerations of up to 600 G with low thermal sensitivity, indicating that tongue projection is powered by an elastic-recoil mechanism...
May 20, 2018: Journal of Experimental Zoology. Part A, Ecological and Integrative Physiology
https://www.readbyqxmd.com/read/29776384/competence-of-mosquitoes-native-to-the-united-kingdom-to-support-replication-and-transmission-of-rift-valley-fever-virus
#3
Sarah Lumley, Luis M Hernández-Triana, Daniel L Horton, Maria Del Mar Fernández de Marco, Jolyon M Medlock, Roger Hewson, Anthony R Fooks, Nicholas Johnson
BACKGROUND: Rift Valley fever phlebovirus (RVFV) is a mosquito-borne arbovirus causing severe disease in humans and livestock. It is endemic in Africa and spread to the Arabian Peninsula in 2000 raising concerns it could emerge in Europe. The ability of temperate mosquitoes from the United Kingdom (UK) to support replication and transmission of RVFV is unknown. METHODS: In this study, two colonised lines of Culex pipiens, wild-caught Aedes detritus and Ae. rusticus from the UK were infected with pathogenic strains of RVFV to assess their vector competence...
May 18, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29776054/least-rattling-feedback-from-strong-time-scale-separation
#4
Pavel Chvykov, Jeremy England
In most interacting many-body systems associated with some "emergent phenomena," we can identify subgroups of degrees of freedom that relax on dramatically different time scales. Time-scale separation of this kind is particularly helpful in nonequilibrium systems where only the fast variables are subjected to external driving; in such a case, it may be shown through elimination of fast variables that the slow coordinates effectively experience a thermal bath of spatially varying temperature. In this paper, we investigate how such a temperature landscape arises according to how the slow variables affect the character of the driven quasisteady state reached by the fast variables...
March 2018: Physical Review. E
https://www.readbyqxmd.com/read/29775352/three-dimensional-color-code-thresholds-via-statistical-mechanical-mapping
#5
Aleksander Kubica, Michael E Beverland, Fernando Brandão, John Preskill, Krysta M Svore
Three-dimensional (3D) color codes have advantages for fault-tolerant quantum computing, such as protected quantum gates with relatively low overhead and robustness against imperfect measurement of error syndromes. Here we investigate the storage threshold error rates for bit-flip and phase-flip noise in the 3D color code (3DCC) on the body-centered cubic lattice, assuming perfect syndrome measurements. In particular, by exploiting a connection between error correction and statistical mechanics, we estimate the threshold for 1D stringlike and 2D sheetlike logical operators to be p_{3DCC}^{(1)}≃1...
May 4, 2018: Physical Review Letters
https://www.readbyqxmd.com/read/29763713/practice-of-mechanical-ventilation-in-cardiac-arrest-patients-and-effects-of-targeted-temperature-management-a-substudy-of-the-targeted-temperature-management-trial
#6
Matthew B A Harmon, David M P van Meenen, Annelou L I P van der Veen, Jan M Binnenkade, Josef Dankiewicz, Florian Ebner, Niklas Nielsen, Paolo Pelosi, Marcus J Schultz, Janneke Horn, Hans Friberg, Nicole P Juffermans
AIMS: Mechanical ventilation practices in patients with cardiac arrest are not well described. Also, the effect of temperature on mechanical ventilation settings is not known. The aims of this study were 1) to describe practice of mechanical ventilation and its relation with outcome 2) to determine effects of different target temperatures strategies (33 °C versus 36 °C) on mechanical ventilation settings. METHODS: This is a substudy of the TTM-trial in which unconscious survivors of a cardiac arrest due to a cardiac cause were randomized to two TTM strategies, 33 °C (TTM33) and 36 °C (TTM36)...
May 12, 2018: Resuscitation
https://www.readbyqxmd.com/read/29757996/design-of-a-low-power-small-area-aec-q100-compliant-sent-transmitter-in-signal-conditioning-ic-for-automotive-pressure-and-temperature-complex-sensors-in-180-nm-cmos-technology
#7
Imran Ali, Behnam Samadpoor Rikhan, Dong-Gyu Kim, Dong-Soo Lee, Muhammad Riaz Ur Rehman, Hamed Abbasizadeh, Muhammad Asif, Minjae Lee, Keum Cheol Hwang, Youngoo Yang, Kang-Yoon Lee
In this paper, a low-power and small-area Single Edge Nibble Transmission (SENT) transmitter design is proposed for automotive pressure and temperature complex sensor applications. To reduce the cost and size of the hardware, the pressure and temperature information is processed with a single integrated circuit (IC) and transmitted at the same time to the electronic control unit (ECU) through SENT. Due to its digital nature, it is immune to noise, has reduced sensitivity to electromagnetic interference (EMI), and generates low EMI...
May 14, 2018: Sensors
https://www.readbyqxmd.com/read/29755425/identification-and-characterization-of-c-di-gmp-metabolic-enzymes-of-leptospira-interrogans-and-c-di-gmp-fluctuations-after-thermal-shift-and-infection
#8
Guohui Xiao, Liangliang Kong, Rongbo Che, Yusi Yi, Qinchao Zhang, Jie Yan, Xu'ai Lin
Leptospirosis is a widespread zoonotic disease caused by pathogenic Leptospira species. The most common species, Leptospira interrogans , can transfer from contaminated soil or water to the human body. It is able to survive these changing environments through sensing and responding to the changes of environmental cues. Cyclic di-GMP (c-di-GMP) is a special secondary messenger in bacteria, which can respond to the environment and regulate diverse bacterial behaviors. The c-di-GMP levels in bacterial cells are regulated by diguanylatecyclases (DGC) and phosphodiesterases (PDE), which are responsible for synthesizing or hydrolyzing c-di-GMP, respectively...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29751262/the-effect-of-580-nm-based-led-mixed-light-on-growth-adipose-deposition-skeletal-development-and-body-temperature-of-chickens
#9
Yefeng Yang, Chenghuang Zhen, Bo Yang, Yonghua Yu, Jinming Pan
Though previous study indicated that the 580 nm-yellow-LED-light showed an stimulating effect on growth of chickens, the low luminous efficiency of the yellow LED light cannot reflect the advantage of energy saving. In present study, the cool white LED chips and yellow LED chips have been combined to fabricate the white × yellow mixed LED light, with an enhanced luminous efficiency. A total 300 newly hatched chickens were reared under various mixed LED light. The results indicated that the white × yellow mixed LED light had "double-edged sword" effects on bird's body weight, bone development, adipose deposition, and body temperature, depending on variations in ratios of yellow component...
April 27, 2018: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29751056/sex-and-trait-anxiety-differences-in-psychological-stress-are-modified-by-environment
#10
James Liu, John Scira, Simone Donaldson, Nina Kajiji, Gordon H Dash, S Tiffany Donaldson
Evidence-based research has revealed how physiological and emotional responses to acute stress are adaptive. However, under conditions of unpredictable or protracted stress, health and drug vulnerability can be compromised. In this study, we examined anxiety-like behavioral responses of 4th generation adolescent male and female Long Evans rats selectively bred for high (HAn) and low (LAn) anxiety-like behavior when housed in an isolated environment (IE) versus a social environment (SE). After 35 days in IE or SE, animals were tested in the elevated plus maze (EPM), injected with amphetamine (AMPH: 0...
May 8, 2018: Neuroscience
https://www.readbyqxmd.com/read/29748215/high-resting-metabolic-rates-with-low-thermal-dependence-induce-active-dives-in-overwintering-pacific-juvenile-loggerhead-turtles
#11
Chihiro Kinoshita, Takuya Fukuoka, Yasuaki Niizuma, Tomoko Narazaki, Katsufumi Sato
The metabolic rate and activity of sea turtles generally decreases with decreasing seasonal ambient temperature. Juvenile loggerhead turtles in the Mediterranean Sea made prolonged inactive dives (>400 min), indicating a state of dormancy during the cold winter period. However, seasonal differences in dive duration were not detected in juvenile loggerheads in the western North Pacific, even though the ambient water temperature changed by more than 10°C. Thus, metabolic states might differ among populations, explaining differences in the diving behaviour of juveniles during winter...
May 10, 2018: Journal of Experimental Biology
https://www.readbyqxmd.com/read/29746908/the-behavioral-energetics-of-new-zealand-s-bats-daily-torpor-and-hibernation-a-continuum
#12
Brian K McNab, Colin O'Donnell
We examine the impact of behavior on the short-term energy expenditures of the only terrestrial mammals endemic to New Zealand, two bats, the long-tailed (Chalinolobus tuberculatus, family Vespertilionidae), and the lesser short-tailed (Mystacina tuberculata, family Mystacinidae). Vespertilionidae has a world-wide distribution. Mystacinidae is restricted to New Zealand, although related to five neotropical families and one in Madagascar reflecting a shared Gondwanan origin of their Noctilionoidea superfamily...
May 7, 2018: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/29746218/effect-of-an-innovative-technology-active-warming-and-passive-warming-on-unplanned-hypothermia-during-perioperative-period-a-clinical-trial
#13
Ganime Esra Soysal, Arzu İlçe, Mehmet Hayri Erkol
Patients are at risk for unplanned hypothermia during the perioperative period due to many reasons, including anesthesia, low room temperature, cold intravenous fluid, and blood transfusion. This study was conducted to examine the effect of active and passive warming methods applied in patients during the perioperative period on unplanned hypothermia. This study is a case-control type study. The population of this study is composed of the patients hospitalized in surgical clinic and undergone abdominal region-related operations...
May 10, 2018: Therapeutic Hypothermia and Temperature Management
https://www.readbyqxmd.com/read/29745775/both-low-temperature-and-shorter-duration-of-food-availability-delay-testicular-regression-and-affect-the-daily-cycle-in-body-temperature-in-a-songbird
#14
Alistair Dawson
Photoperiodic control of reproduction in birds is based on two processes, a positive effect leading to gonadal maturation and an inhibitory effect subsequently inducing regression. Nonphotoperiodic cues can modulate photoperiodic control, particularly the inhibitory process. In previous studies of common starlings (Sturnus vulgaris), (1) restriction of food availability to 8 h after dawn had little effect on testicular maturation but dramatically delayed subsequent regression and (2) lower ambient temperature also had little effect during maturation but delayed regression...
July 2018: Physiological and Biochemical Zoology: PBZ
https://www.readbyqxmd.com/read/29734436/cloning-and-expression-analysis-of-glucose-transporter-4-mrna-in-the-cold-hardiness-frog-rana-dybowskii
#15
B H Guo, S S Gong, J Y Zhang, L H Chai, Y Zhang, B J Wang, J Shao, X H Xiao
BACKGROUND: The Rana dybowskii distribute in northeast region of China which have seasonally cold climates. During winter they survival freezing by biosynthesizing carbohydrate cryoprotectants such as high concentrations glucose into blood and all tissues. The essential role of glucose transporter 4 is a high-affinity glucose transporter, which can increase glucose uptake in cells when it stimulated by insulin. OBJECTIVE: In this study, we analysis the full-length GLUT4 mRNA detect the gene levels of GLUT4 in R...
July 2017: Cryo Letters
https://www.readbyqxmd.com/read/29728762/what-may-a-fussy-creature-reveal-about-body-cell-size-integration-under-stressful-conditions
#16
Aleksandra Walczyńska, Anna Maria Labecka, Mateusz Sobczyk
There is a growing amount of empirical evidence on the important role of cell size in body size adjustment in ambient or changing conditions. Though the adaptive significance of their correspondence is well understood and demonstrated, the proximate mechanisms are still in a phase of speculation. We made interesting observations on body/cell size adjustment under stressful conditions during an experiment designed for another purpose. We found that the strength of the body/cell size match is condition-dependent...
May 4, 2018: Development Genes and Evolution
https://www.readbyqxmd.com/read/29727362/effect-of-body-temperature-on-cerebral-autoregulation-in-acutely-comatose-neurocritically-ill-patients
#17
Krishma Adatia, Romergryko G Geocadin, Ryan Healy, Wendy Ziai, Luciano Ponce-Mejia, Mirinda Anderson-White, Dhaval Shah, Batya R Radzik, Caitlin Palmisano, Charles W Hogue, Charles Brown, Lucia Rivera-Lara
OBJECTIVES: Impaired cerebral autoregulation following neurologic injury is a predictor of poor clinical outcome. We aimed to assess the relationship between body temperature and cerebral autoregulation in comatose patients. DESIGN: Retrospective analysis of prospectively collected data. SETTING: Neurocritical care unit of the Johns Hopkins Hospital. PATIENTS: Eighty-five acutely comatose patients (Glasgow Coma Scale score of ≤ 8) admitted between 2013 and 2017...
May 4, 2018: Critical Care Medicine
https://www.readbyqxmd.com/read/29723112/effect-of-green-coffee-consumption-on-resting-energy-expenditure-blood-pressure-and-body-temperature-in-healthy-women-a-pilot-study
#18
Nilüfer Acar-Tek, Duygu Agagüundüz, Büşra Ayhan
OBJECTIVE: This study was conducted to determine the effects of green coffee consumption on resting energy expenditures (REEs), blood pressure, and body temperature of individuals. METHOD: The study was conducted with 24 women. The REE values of the individuals were measured with the COSMED Fitmate PRO. After the first REE measurements, individuals were given 1 cup of green coffee that was prepared to contain 6 mg caffeine per kg of lean body mass. After coffee consumption, REE measurements were made at 30, 60, 120, and 180 minutes...
May 3, 2018: Journal of the American College of Nutrition
https://www.readbyqxmd.com/read/29722138/behavioural-thermoregulation-is-highly-repeatable-and-unaffected-by-digestive-status-in-agama-atra
#19
Jenna van Berkel, Susana Clusella-Trullas
The precision and the extent of behavioral thermoregulation are likely to provide fitness benefits to ectotherms. Yet the factors driving variation in selected or preferred body temperature (Tset ) and its usefulness as a proxy for optimal physiological temperature (Topt ) are still debated. Although Tset is often conserved among closely related species, substantial variation at the individual, population, and species level has also been reported but repeatability (sensu the intra-class correlation coefficient, ICC) of Tset is generally low...
May 3, 2018: Integrative Zoology
https://www.readbyqxmd.com/read/29721303/seasonal-variations-in-body-melanism-and-size-of-the-wolf-spider-pardosa-astrigera-araneae-lycosidae
#20
Jinjian Yang, Qijia Wu, Rong Xiao, Jupeng Zhao, Jian Chen, Xiaoguo Jiao
Variations in species morphology and life-history traits strongly correlate with geographic and climatic characteristics. Most studies on morphological variations in animals focus on ectotherms distributed on a large geographic scale across latitudinal and/or altitudinal gradient. However, the morphological variations of spiders living in the same habitats across different seasons have not been reported. In this study, we used the wolf spider, Pardosa astrigera , as a model to determine seasonal differences in adult body size, melanism, fecundity, and egg diameter both in the overwintering and the first generation for 2010 and 2016...
April 2018: Ecology and Evolution
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