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https://www.readbyqxmd.com/read/29149665/a-study-of-microbial-communities-on-terracotta-separator-and-on-biocathode-of-air-breathing-microbial-fuel-cells
#1
Laura Rago, Sarah Zecchin, Stefania Marzorati, Andrea Goglio, Lucia Cavalca, Pierangela Cristiani, Andrea Schievano
Recently, terracotta has attracted interest as low-cost and biocompatible material to build separators in microbial fuel cells (MFCs). However, the influence of a non-conductive material like terracotta on electroactive microbiological communities remains substantially unexplored. This study aims at describing the microbial pools developed from two different seed inocula (bovine and swine sewage) in terracotta-based air-breathing MFC. A statistical approach on microbiological data confirmed different community enrichment in the MFCs, depending mainly on the inoculum...
November 11, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/29149627/a-simple-and-sensitive-two-tube-multiplex-pcr-assay-for-simultaneous-detection-of-ten-meat-species
#2
Olga V Prusakova, Xenia A Glukhova, Gaida V Afanas'eva, Yulia A Trizna, Ludmila F Nazarova, Igor P Beletsky
A sensitive and reliable technique for meat species identification is required to prevent food adulteration, particularly in meat production. This work developed an optimized multiplex PCR assay for simultaneous identification of five commonly consumed and five commonly banned meat species in meat products. We designed primers that specifically amplified mitochondrial ATPase subunit 8 gene regions of different lengths of bovine, ovine, swine, chicken, turkey, cat, dog, mouse and human DNAs. The developed multiplex PCR assay proved to be specific, sensitive down to 30pg DNA per reaction, reproducible and economical...
October 26, 2017: Meat Science
https://www.readbyqxmd.com/read/29148391/outbreaks-of-neuroinvasive-astrovirus-associated-with-encephalomyelitis-weakness-and-paralysis-among-weaned-pigs-hungary
#3
Ákos Boros, Mihály Albert, Péter Pankovics, Hunor Bíró, Patricia A Pesavento, Tung Gia Phan, Eric Delwart, Gábor Reuter
A large, highly prolific swine farm in Hungary had a 2-year history of neurologic disease among newly weaned (25- to 35-day-old) pigs, with clinical signs of posterior paraplegia and a high mortality rate. Affected pigs that were necropsied had encephalomyelitis and neural necrosis. Porcine astrovirus type 3 was identified by reverse transcription PCR and in situ hybridization in brain and spinal cord samples in 6 animals from this farm. Among tissues tested by quantitative RT-PCR, the highest viral loads were detected in brain stem and spinal cord...
December 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29148383/porcine-astrovirus-type-3-in-central-nervous-system-of-swine-with-polioencephalomyelitis
#4
Bailey Arruda, Paulo Arruda, Melissa Hensch, Qi Chen, Ying Zheng, Chenghuai Yang, Igor Renan Honorato Gatto, Franco Matias Ferreyra, Phil Gauger, Kent Schwartz, Laura Bradner, Karen Harmon, Ben Hause, Ganwu Li
Using next-generation sequencing, we identified and genetically characterized a porcine astrovirus type 3 strain found in tissues from the central nervous system of 1 piglet and 3 sows with neurologic signs and nonsuppurative polioencephalomyelitis. Further studies are needed to understand the potential for cross-species transmission and clinical impact.
December 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29148297/detection-of-pseudorabies-virus-by-duplex-droplet-digital-pcr-assay
#5
Meishen Ren, Hua Lin, Shijie Chen, Miao Yang, Wei An, Yin Wang, Changhua Xue, Yinjie Sun, Yubao Yan, Juan Hu
Aujeszky's disease, caused by pseudorabies virus (PRV), has damaged the economy of the Chinese swine industry. A large number of PRV gene-deleted vaccines have been constructed based on deletion of the glycoprotein E ( gE) gene combined with other virulence-related gene deletions, such as thymidine kinase ( TK), whereas PRV wild-type strains contain an intact gE gene. We developed a sensitive duplex droplet digital PCR (ddPCR) assay to rapidly detect PRV wild-type isolates and gE gene-deleted viral vaccines...
November 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29148267/detection-of-teschovirus-type-13-from-two-swine-herds-exhibiting-nervous-clinical-signs-in-growing-pigs
#6
J Carnero, C Prieto, L Polledo, F J Martínez-Lobo
Recently, the number of clinical reports of growing pigs showing neurological signs possibly related to viral infections has increased. The objective of this report was to describe two outbreaks of an atypical condition observed in 6- to 7-week-old pigs with a morbidity of 20% and a fatality rate of 60% in two unrelated farms of the same company. During the acute phase of the disease, fever, sudden death, neurological signs, ear necrosis and occasional corneal opacity were observed. Histopathological examination revealed interstitial pneumonia, lymphoid depletion and lymphocytic vasculitis in different organs and mild polioencephalomyelitis suggesting a potential viral infection...
November 16, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29147809/application-of-actuator-driven-pulsed-water-jet-for-coronary-artery-bypass-grafting-assessment-in-a-swine-model
#7
Tomoyuki Suzuki, Shunsuke Kawamoto, Atsuhiro Nakagawa, Toshiki Endo, Teiji Tominaga, Masatoshi Akiyama, Osamu Adachi, Kiichiro Kumagai, Yoshikatsu Saiki
Actuator-driven pulsed water-jet (ADPJ) dissection is an emerging surgical method for dissecting tissue without heat and mechanical injury to vessels. We elucidated the mechanical properties of the piezo ADPJ and evaluated its usefulness and safety in coronary artery bypass grafting procedures. The relationship between the input voltage (10-100 V) and peak pressure of the pulsed water jet was evaluated. The tissue strengths of swine internal thoracic and coronary arteries and the surrounding tissues were measured to assure tissue-selective dissection...
November 16, 2017: Journal of Artificial Organs: the Official Journal of the Japanese Society for Artificial Organs
https://www.readbyqxmd.com/read/29147640/robinsoniella-peoriensis-infection-related-to-right-femoral-hardware
#8
Justin Schmetterer, Joseph Gorvetzian, Shaun Yang
Discovered in swine manure relatively recently, Robsinoniella peoriensis is a gram-positive anaerobic bacilli that has been reported to cause human infections. Its antimicrobial susceptibility, epidemiology and overall pathogenicity are still not fully understood. We report a case of a hardware related soft tissue infection in the right femur caused by Robinsoneilla peoriensis following an open reduction internal fixation in a 67 year old immunocompetent woman. The patient was successfully treated with six weeks of ertapenem...
2017: IDCases
https://www.readbyqxmd.com/read/29147092/meat-quality-traits-of-pigs-finished-on-food-waste
#9
Jihwan Choe, Knowledge M Moyo, Kibum Park, Jeongho Jeong, Haeun Kim, Yungsun Ryu, Jonggun Kim, Jun-Mo Kim, Sanghoon Lee, Gwang-Woong Go
Despite the benefits associated with the use of food waste (FW), there are mixed consumer perceptions regarding pork quality harvested from pigs fed FW. Twenty crossbred pigs were selected for the present study. Ten pigs were fed a conventional diet (control group), and the other 10 pigs were given a conventional diet and FW (FW group) during different growth stages. Meat quality in the FW group showed deteriorative qualities with higher lightness and yellowness synonymous to pale soft exudative meat. Drip loss in the experimental group was significantly higher than that in the control group (p<0...
2017: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/29145950/a-combination-of-allogeneic-stem-cells-promotes-cardiac-regeneration
#10
Makoto Natsumeda, Victoria Florea, Angela C Rieger, Bryon A Tompkins, Monisha N Banerjee, Samuel Golpanian, Julia Fritsch, Ana Marie Landin, Nilesh D Kashikar, Vasileios Karantalis, Viky Y Loescher, Kostas E Hatzistergos, Luiza Bagno, Cristina Sanina, Muzammil Mushtaq, Jose Rodriguez, Marcos Rosado, Ariel Wolf, Kevin Collon, Louis Vincent, Anthony J Kanelidis, Ivonne H Schulman, Raul Mitrani, Alan W Heldman, Wayne Balkan, Joshua M Hare
BACKGROUND: The combination of autologous mesenchymal stem cells (MSCs) and cardiac stem cells (CSCs) synergistically reduces scar size and improves cardiac function in ischemic cardiomyopathy. Whereas allogeneic (allo-)MSCs are immunoevasive, the capacity of CSCs to similarly elude the immune system remains controversial, potentially limiting the success of allogeneic cell combination therapy (ACCT). OBJECTIVES: This study sought to test the hypothesis that ACCT synergistically promotes cardiac regeneration without provoking immunologic reactions...
November 14, 2017: Journal of the American College of Cardiology
https://www.readbyqxmd.com/read/29145911/hepatitis-e-virus-infection-in-north-italy-high-seroprevalence-in-swine-herds-and-increased-risk-for-swine-workers
#11
L Mughini-Gras, G Angeloni, C Salata, N Vonesch, W D'Amico, G Campagna, A Natale, F Zuliani, L Ceglie, I Monne, M Vascellari, K Capello, G DI Martino, N Inglese, G Palù, P Tomao, L Bonfanti
We determined the hepatitis E virus (HEV) seroprevalence and detection rate in commercial swine herds in Italy's utmost pig-rich area, and assessed HEV seropositivity risk in humans as a function of occupational exposure to pigs, diet, foreign travel, medical history and hunting activities. During 2011-2014, 2700 sera from 300 swine herds were tested for anti-HEV IgG. HEV RNA was searched in 959 faecal pools from HEV-seropositive herds and in liver/bile/muscle samples from 179 pigs from HEV-positive herds. A cohort study of HEV seropositivity in swine workers (n = 149) was also performed using two comparison groups of people unexposed to swine: omnivores (n = 121) and vegetarians/vegans (n = 115)...
November 17, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/29145208/curcumin-delays-retinal-degeneration-by-regulating-microglia-activation-in-the-retina-of-rd1-mice
#12
Yanhe Wang, Zhiyuan Yin, Lixiong Gao, Dayu Sun, Xisu Hu, Langyue Xue, Jiaman Dai, YuXiao Zeng, Siyu Chen, Boju Pan, Min Chen, Jing Xie, Haiwei Xu
BACKGROUND/AIMS: Retinitis pigmentosa (RP) is characterized by degeneration of photoreceptors, and there are currently no effective treatments for this disease. However, curcumin has shown neuroprotectant efficacy in a RP rat and swine model, and thus, may have neuroprotective effects in this disease. METHODS: Immunofluorescence staining, electroretinogram recordings, and behavioral tests were used to analyze the effects of curcumin and the underlying mechanism in retinal degeneration 1 (rd1) mice...
November 17, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29145163/spread-of-multidrug-resistant-escherichia-coli-harboring-integron-via-swine-farm-waste-water-treatment-plant
#13
Jin-Hyeong Park, Young-Ji Kim, Binn-Kim, Kun-Ho Seo
Wastewater treatment plants (WWTPs) that release treated wastewater into the environment have emerged as a major threat to public health. In this study, we investigated Escherichia coli load and antibiotic-resistance profiles across different treatment processes at a swine farm WWTP. The frequency of the detection of class 1 and 2 integrons, and their association with antibiotic resistance, were also analyzed. Samples were obtained at each of five sampling sites that represented each processing step within the WWTP...
November 13, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29144584/3d-printed-anchoring-sutures-for-permanent-shaping-of-tissues
#14
Wei Wei, Yuxiao Li, Huazhe Yang, Reza Nassab, Fatemeh Shahriyari, Ali Akpek, Xiaofei Guan, Yanhui Liu, Shahrouz Taranejoo, Ali Tamayol, Yu Shrike Zhang, Ali Khademhosseini, Hae Lin Jang
Sutures are one of the most widely used devices for adhering separated tissues after injury or surgery. However, most sutures require knotting, which can create a risk of inflammation, and can act as mechanically weak points that often result in breakage and slipping. Here, an anchoring suture is presented with a design that facilitates its propagation parallel to the suturing direction, while maximizing its resistive force against the opposite direction of external force to lock its position in tissues. Different microstructures of suture anchors are systematically designed using orthogonal arrays, and selected based on shape factors associated with mechanical strength...
November 16, 2017: Macromolecular Bioscience
https://www.readbyqxmd.com/read/29143703/dose-response-evaluation-of-the-standardized-ileal-digestible-tryptophan-lysine-ratio-to-maximize-growth-performance-of-growing-finishing-gilts-under-commercial-conditions
#15
M A D Gonçalves, M D Tokach, N M Bello, K J Touchette, R D Goodband, J M DeRouchey, J C Woodworth, S S Dritz
Environmental regulations as well as economic incentives have resulted in greater use of synthetic amino acids in swine diets. Tryptophan is typically the second limiting amino acid in corn-soybean meal-based diets. However, using corn-based co-products emphasizes the need to evaluate the pig's response to increasing Trp concentrations. Therefore, the objective of these studies was to evaluate the dose-response to increasing standardized ileal digestible (SID) Trp : Lys on growth performance of growing-finishing gilts housed under large-scale commercial conditions...
November 16, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/29143461/do-animal-exhibitors-support-and-follow-recommendations-to-prevent-transmission-of-variant-influenza-at-agricultural-fairs-a-survey-of-animal-exhibitor-households-after-a-variant-influenza-virus-outbreak-in-michigan
#16
R J Stewart, J Rossow, J T Conover, E E Lobelo, S Eckel, K Signs, M G Stobierski, S C Trock, A M Fry, S J Olsen, M Biggerstaff
Influenza A viruses circulate in swine and can spread rapidly among swine when housed in close proximity, such as at agricultural fairs. Youth who have close and prolonged contact with influenza-infected swine at agricultural fairs may be at increased risk of acquiring influenza virus infection from swine. Animal and human health officials have issued written measures to minimize influenza transmission at agricultural exhibitions; however, there is little information on the knowledge, attitudes, and practice (KAP) of these measures among animal exhibitors...
November 16, 2017: Zoonoses and Public Health
https://www.readbyqxmd.com/read/29143334/pk-pd-modeling-of-flunixin-meglumine-in-a-kaolin-induced-inflammation-model-in-piglets
#17
O L Levionnois, T K Fosse, B Ranheim
Flunixin is marketed in several countries for analgesia in adult swine but little is known about its efficacy in piglets. Thirty-two piglets (6-8 days old) were randomized to receive placebo saline (n = 11, group CONTROL) or flunixin meglumine intravenously at 2.2 (n = 11, group MEDIUM) or 4.4 (n = 10, group HIGH) mg/kg, 10 hr after subcutaneous injection of kaolin in the left metacarpal area. A hand-held algometer was used to determine each piglet's mechanical nociceptive threshold (MNT) from both front feet up to 50 hr after treatment (cut-off value of 24...
November 16, 2017: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/29143187/multi-locus-sequence-typing-of-african-swine-fever-viruses-from-endemic-regions-of-kenya-and-eastern-uganda-2011-2013-reveals-rapid-b602l-central-variable-region-evolution
#18
C K Onzere, A D Bastos, E A Okoth, J K Lichoti, E N Bochere, M G Owido, G Ndambuki, M Bronsvoort, R P Bishop
The central variable region (CVR) within the B602L gene of the African swine fever virus (ASFV) is highly polymorphic within the 23 ASFV genotypes defined by sequencing of the C-terminal end of the p72 locus. Sequencing the p54 gene further discriminates ASFV genotypes that are conserved at the p72 locus. Variation in the thymidine kinase locus is a novel additional tool for ASFV genotyping whose application for this purpose is described for the first time herein. We evaluated genetic variation at these four polymorphic loci in 39 ASFV isolates obtained from outbreaks in Kenya and a region of Eastern Uganda between 2011 and 2013...
November 15, 2017: Virus Genes
https://www.readbyqxmd.com/read/29142249/a-magnetoelastic-biosensor-based-on-e2-glycoprotein-for-wireless-detection-of-classical-swine-fever-virus-e2-antibody
#19
Xing Guo, Shengbo Sang, Jinyu Guo, Aoqun Jian, Qianqian Duan, Jianlong Ji, Qiang Zhang, Wendong Zhang
A wireless magnetoelastic (ME) biosensor immobilized with E2 glycoprotein was first developed to detect classical swine fever virus (CSFV) E2 antibody. The detection principle is that a sandwich complex of CSFV E2 - rabbit anti-CSFV E2 antibody - alkaline phosphatase (AP) conjugated goat anti-rabbit IgG formed on the ME sensor surface, with biocatalytic precipitation used to amplify the mass change of antigen-antibody specific binding reaction, induces a significant change in resonance frequency of the biosensor...
November 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29142159/systemic-cytokine-response-in-pigs-infected-orally-with-a-lawsonia-intracellularis-isolate-of-south-korean-origin
#20
Jung-Yong Yeh, A-Reum Ga
In the swine industry, Lawsonia intracellularis is one of the main enteric pathogens; it causes acute intestinal hemorrhage (proliferative hemorrhagic enteropathy) in naïve adult pigs and a wasting disease (proliferative enteropathy) in growing pigs. Among many kinds of cytokines, interferon-γ (IFN-γ) has previously been reported to play a significant role in limiting intracellular infection and increasing cellular proliferation associated with L. intracellularis. However, the levels of various circulating inflammatory cytokines, including IFN-γ, in animals infected with L...
November 15, 2017: Journal of Veterinary Medical Science
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