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https://www.readbyqxmd.com/read/28087375/super-oxidized-water-inactivates-major-viruses-circulating-in-swine-farms
#1
Jianing Chen, Chengyu Zhang, Yue Liu, Guangliang Liu
Disinfectant is commonly employed to eliminate infectious agents and prevent its transmission. In this study, we investigated the efficacy of Medilox(®) super-oxidized water on inactivating veterinary viruses mainly circulating in swine farms. The results demonstrated that this super-oxidized water could effectively inactivate porcine viruses.
January 10, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28081386/effects-of-biodiesel-made-from-swine-and-chicken-fat-residues-on-co-co2-and-nox-emissions
#2
Vivian Feddern, Anildo Cunha Junior, Marina C De Prá, Marcio L Busi da Silva, Rodrigo da S Nicoloso, Martha M Higarashi, Arlei Coldebella, Paulo G de Abreu
Implications Emissions from motor vehicles can contribute considerably to the levels of greenhouse gases in the atmosphere. The use of biodiesel to replace or augment diesel can not only decrease our dependency on fossil fuels but also help decrease air pollution. Thus, different sources of feedstocks are constantly being explored for affordable biodiesel production. However, the amount of CO, CO2 and/or NOx emissions can vary largely depending on type of feedstock used to produce biodiesel. In this work we demonstrated animal fat feasibility in replacing petrodiesel with less impact regarding greenhouse gas emissions than other sources...
January 12, 2017: Journal of the Air & Waste Management Association
https://www.readbyqxmd.com/read/28081156/generation-of-a-stable-transgenic-swine-model-expressing-a-porcine-histone-2b-egfp-fusion-protein-for-cell-tracking-and-chromosome-dynamics-studies
#3
Renan B Sper, Sehwon Koh, Xia Zhang, Sean Simpson, Bruce Collins, Jeff Sommer, Robert M Petters, Ignacio Caballero, Jeff L Platt, Jorge A Piedrahita
Transgenic pigs have become an attractive research model in the field of translational research, regenerative medicine, and stem cell therapy due to their anatomic, genetic and physiological similarities with humans. The development of fluorescent proteins as molecular tags has allowed investigators to track cell migration and engraftment levels after transplantation. Here we describe the development of two transgenic pig models via SCNT expressing a fusion protein composed of eGFP and porcine Histone 2B (pH2B)...
2017: PloS One
https://www.readbyqxmd.com/read/28081123/yersinia-enterocolitica-a-neglected-cause-of-human-enteric-infections-in-c%C3%A3-te-d-ivoire
#4
Daniel Saraka, Cyril Savin, Stephane Kouassi, Bakary Cissé, Eugène Koffi, Nicolas Cabanel, Sylvie Brémont, Hortense Faye-Kette, Mireille Dosso, Elisabeth Carniel
BACKGROUND: Enteropathogenic Yersinia circulate in the pig reservoir and are the third bacterial cause of human gastrointestinal infections in Europe. In West Africa, reports of human yersiniosis are rare. This study was conducted to determine whether pathogenic Yersinia are circulating in pig farms and are responsible for human infections in the Abidjan District. METHODOLOGY/PRINCIPAL FINDINGS: From June 2012 to December 2013, pig feces were collected monthly in 41 swine farms of the Abidjan district...
January 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28077800/oxidative-stress-induced-by-se-deficient-high-energy-diet-implicates-neutrophil-dysfunction-via-nrf2-pathway-suppression-in-swine
#5
Tianshu Yang, Zeping Zhao, Tianqi Liu, Ziwei Zhang, Pengzu Wang, Shiwen Xu, Xin Gen Len, Anshan Shan
The mechanism of the interaction between Se deficiency and high energy remains limited. The aim of the current study was to identify whether Se-deficient, high-energy diet can induce oxidative stress, and downregulate the Nrf2 pathway and phagocytic dysfunction of neutrophils. We detected the phagocytic activity, ROS production, protein levels of Nrf2 and Nrf2 downstream target genes, and the mRNA levels of 25 selenoproteins, heat shock proteins, and cytokines in neutrophils. Cytokine ELISA kits were used to measure the serum cytokines...
January 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28077698/detection-of-antigenic-variants-of-swine-subtype-h3-influenza-a-viruses-from-clinical-samples
#6
Brigitte E Martin, Andrew S Bowman, Lei Li, Jacqueline M Nolting, David R Smith, Larry A Hanson, Xiu-Feng Wan
A large population of genetically and antigenically diverse influenza A viruses (IAVs) are circulating among the swine population, playing an important role in influenza ecology. Swine IAVs not only cause outbreaks among swine, but they can also be transmitted to humans, causing sporadic infections and even pandemic outbreaks. Antigenic characterization of swine IAVs are key to understanding the natural history of these viruses in swine and to selecting strains for effective vaccines. However, influenza outbreaks generally spread rapidly among swine, and the conventional methods for antigenic characterization require virus propagation, a time-consuming process that can significantly reduce the effectiveness of vaccination programs...
January 11, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28077590/molecular-epidemiology-of-ascaris-infection-among-pigs-in-iowa
#7
Jeba Jesudoss Chelladurai, Kaitlyn Murphy, Tymbrie Snobl, Christopher Bader, Cody West, Kylie Thompson, Matthew T Brewer
Ascaris is a large roundworm parasite that infects humans and pigs throughout the world. Molecular markers have been used to study parasite transmission in Ascaris-endemic and -nonendemic regions of the world. In the United States, ascariasis still persists in commercial swine and has been designated a neglected disease of poverty in humans. However, relatively few data are available for evaluation of zoonotic transmission. In the present study, we obtained adult worms from abattoirs and characterized each worm on the basis of the gene encoding nuclear internal transcribed sequence (ITS) and mitochondrial cox1 Restriction fragment-length polymorphism analysis of ITS revealed swine, human, and hybrid genotypes...
January 1, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28076681/computational-modeling-of-neurotransmitter-release-evoked-by-electrical-stimulation-non-linear-approaches-to-predicting-stimulation-evoked-dopamine-release
#8
James K Trevathan, Ali Yousefi, Hyung Ook Park, John J Bartoletta, Kip A Ludwig, Kendall H Lee, J Luis Lujan
Neurochemical changes evoked by electrical stimulation of the nervous system have been linked to both therapeutic and undesired effects of neuromodulation therapies used to treat obsessive-compulsive disorder, depression, epilepsy, Parkinson's disease, stroke, hypertension, tinnitus, and many other indications. In fact, interest in better understanding the role of neurochemical signaling in neuromodulation therapies has been a focus of recent government- and industry-sponsored programs whose ultimate goal is to usher in an era of personalized medicine by creating neuromodulation therapies that respond to real-time changes in patient status...
January 11, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/28076621/experimental-model-for-sutureless-proximal-anastomosis-by-the-viabahn-open-revascularization-technique-vortec
#9
Lucas Marcelo Dias Freire, Giuliana Biasi Gobbi, Inácio Maria Dal Fabbro, Fábio Hüsemann Menezes
Introduction: In the treatment of complex aneurysms, debranching is an extra-anatomical revascularization of visceral arteries followed by endograft coverage of the thoracoabdominal aorta. It eliminates the need for a thoracotomy and aortic clamping, but requires the performance of several technically demanding visceral anastomosis. In 2008, Lachat described visceral revascularization with the use of a sutureless distal anastomosis, performed by the telescoping of an endograft in the visceral branch, named VORTEC (Viabahn Open Revascularization TEChnique)...
November 2016: Brazilian Journal of Cardiovascular Surgery
https://www.readbyqxmd.com/read/28071758/prevalence-of-feral-swine-disturbance-at-important-archaeological-sites-over-a-large-landscape-in-florida
#10
Richard M Engeman, Joseph S Meyer, John B Allen
Feral swine are globally known as one of the most destructive invasive vertebrates, damaging native habitats, native plants and animals, agriculture, infrastructure, spreading diseases. There has been little quantification on their disturbance to archaeological sites across a broad landscape. Over 6 years we inspected 293 significant archaeological sites for swine disturbance across a vast area. We found a 42% prevalence of swine disturbance among all sites, with prevalence not distinguishable among prehistoric sites, historic sites, and sites with both components...
January 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28070330/the-approximated-cardiovascular-reserve-index-complies-with-haemorrhage-related-hemodynamic-deterioration-pattern-a-swine-exsanguination-model
#11
Roy Nadler, Elon Glassberg, Itay E Gabbay, Linn Wagnert-Avraham, Gal Yaniv, David Kushnir, Arik Eisenkraft, Ben-Zion Bobrovsky, Uri Gabbay
BACKGROUND: To estimate the cardiovascular reserve we formulated the Cardiovascular Reserve Index (CVRI) based on physiological measurements. The aim of this study was to evaluate the pattern of CVRI in haemorrhage-related haemodynamic deterioration in an animal model simulating combat injury. METHODS: Data were collected retrospectively from a research database of swine exsanguination model in which serial physiological measurements were made under anesthesia in 12 swine of haemorrhagic injury and 5 controls...
February 2017: Annals of Medicine and Surgery
https://www.readbyqxmd.com/read/28070229/novel-device-for-removal-of-blot-clot-obstructions-in-drainage-tubes
#12
Scott M Thompson, Cornelius A Thiels, Johnathon M Aho
Medical drains frequently become obstructed requiring removal or re-intervention. Currently, there is no widely accepted practice for maintaining drain patency. A simple device to remove obstructions from medical drains may have significant benefit. We manufactured a prototype drain clot extractor (DCE) for extracting obstructions. Fresh nonanticoagulated swine blood was instilled within drainage tubes of 16, 20, and 32 French (n = 2 for each size) to generate a 4 cm blood clot. The operator was then equipped with a 0...
March 2017: Journal of Medical Devices
https://www.readbyqxmd.com/read/28069803/ig-light-chain-precedes-heavy-chain-gene-rearrangement-during-development-of-b-cells-in-swine
#13
Marek Sinkora, Jana Sinkorova, Katerina Stepanova
The current mammalian paradigm states that 1) rearrangements in the IgH locus precede those in IgL loci, 2) IgLλ genes rearrange only when IgLκ genes are consumed, and 3) the surrogate L chain is necessary for selection of productive IgH gene rearrangements. We show in swine that IgL rearrangements precede IgH gene rearrangements, resulting in the expression of naked IgL on a surface of precursor B cells. Findings also suggest that there is no dependency on the surrogate L chain, and thus the authentic IgL proteins may be used for selection of the IgH repertoire...
January 9, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28069290/classical-swine-fever-vaccines-state-of-the-art
#14
Sandra Blome, Claudia Moß, Ilona Reimann, Patricia König, Martin Beer
Due to its impact on animal health and pig industry, classical swine fever (CSF) is still one of the most important viral diseases of pigs. To control the disease, safe and highly efficacious live attenuated vaccines exist for decades. These vaccines have usually outstanding efficacy and safety but lack differentiability of infected from vaccinated animals (DIVA or marker strategy). In contrast, the first generation of E2 subunit marker vaccines shows constraints in efficacy, application, and production. To overcome these limitations, new generations of marker vaccines are developed...
January 3, 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28068884/evaluation-of-protein-spectra-cluster-analysis-for-streptococcus-spp-identification-from-various-swine-clinical-samples
#15
Carlos E C Matajira, Luisa Z Moreno, Vasco T M Gomes, Ana Paula S Silva, Renan E Mesquita, Daniela S Doto, Franco F Calderaro, Fernando N de Souza, Ana Paula G Christ, Maria Inês Z Sato, Andrea M Moreno
Traditional microbiological methods enable genus-level identification of Streptococcus spp. isolates. However, as the species of this genus show broad phenotypic variation, species-level identification or even differentiation within the genus is difficult. Herein we report the evaluation of protein spectra cluster analysis for the identification of Streptococcus species associated with disease in swine by means of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). A total of 250 S...
January 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28067630/novel-swine-model-of-ricin-induced-acute-respiratory-distress-syndrome
#16
Shahaf Katalan, Reut Falach, Amir Rosner, Michael Goldvaser, Tal Brosh-Nissimov, Ayana Dvir, Avi Mizrachi, Orr Goren, Barak Cohen, Yoav Gal, Anita Sapoznikov, Sharon Ehrlich, Tamar Sabo, Chanoch Kronman
Pulmonary exposure to the plant toxin ricin, leads to respiratory insufficiency and death. To date, in-depth study of the functional disorders ensuing pulmonary intoxication, a prerequisite for establishing a clinically-relevant therapeutic protocol, is hampered by the lack of an appropriate animal model. To this end, we set up the pig, as a large animal model for the comprehensive study of the multifarious clinical manifestations of pulmonary ricinosis.Here we report for the first time, the monitoring of barometric whole body plethysmography for pulmonary function tests in non-anesthetized ricin-intoxicated pigs...
January 6, 2017: Disease Models & Mechanisms
https://www.readbyqxmd.com/read/28065395/serial-retrograde-instillations-of-sustained-release-formulation-of-mitomycin-c-to-the-upper-urinary-tract-of-the-yorkshire-swine-using-a-thermosensitive-polymer-safety-and-feasibility
#17
Nicholas M Donin, Dalit Strauss-Ayali, Yael Agmon-Gerstein, Nadav Malchi, Andrew T Lenis, Stuart Holden, Allan J Pantuck, Arie S Belldegrun, Karim Chamie
BACKGROUND: MitoGel is a novel drug formulation intended for the treatment of upper tract urothelial cancer with proven feasibility and safety in an animal model. OBJECTIVE: To evaluate the feasibility, safety, toxicokinetics, and histologic changes associated with serial retrograde MitoGel instillations to the upper urinary tract in a swine model. DESIGN, SETTING, AND PARTICIPANTS: Overall, 27 Yorkshire swine underwent 6 once-weekly unilateral retrograde instillations of MitoGel...
January 3, 2017: Urologic Oncology
https://www.readbyqxmd.com/read/28065162/validation-of-a-competitive-elisa-and-a-virus-neutralization-test-for-the-detection-and-confirmation-of-antibodies-to-senecavirus-a-in-swine-sera
#18
Melissa Goolia, Fabio Vannucci, Ming Yang, Devi Patnayak, Shawn Babiuk, Charles K Nfon
Senecavirus A (SVA; family Picornaviridae) is a nonenveloped, single-stranded RNA virus associated with idiopathic vesicular disease (IVD) in swine. SVA was detected in pigs with IVD in Brazil, United States, Canada, and China in 2015, triggering the need to develop and/or validate serologic assays for SVA. Our objective was to fully validate a previously developed competitive enzyme-linked immunosorbent assay (cELISA) as a screening test for antibodies to SVA. Additional objectives included the development and validation of a virus neutralization test (VNT) as a confirmatory test for SVA antibody detection, and the comparison of the cELISA, VNT, and an existing immunofluorescent antibody test (IFAT) for the detection of SVA antibodies in serial bleeds from SVA outbreaks...
January 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28063923/phylogenetic-analysis-of-hepatitis-e-virus-strains-isolated-from-slaughter-age-pigs-in-colombia
#19
Jorge E Forero D, Cristian Gutiérrez-Vergara, Jaime Parra S, Guillermo Correa L, Berardo Rodríguez, Lina A Gutiérrez, Francisco Javier Díaz, Albeiro López-Herrera
Hepatitis E virus (HEV) is a zoonotic virus that causes acute hepatitis in humans, and can be transmitted via the fecal-oral route. Pigs are considered to be a reservoir for this infection-mainly where the disease is not endemic. In a previous study conducted in Antioquia, which is a region in Colombia where the production and consumption of pork meat is higher than in the rest of the country, the presence of anti-HEV IgG-type antibodies was reported in slaughter-age pigs. Aiming to identify the HEV genotype circulating in swine, animal liver, and feces samples from five swine cattle slaughterhouses located in six different sub-regions of Antioquia were collected...
January 4, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28063479/positive-immunomodulatory-effects-of-heterologous-dna-vaccine-modified-live-vaccine-prime-boost-immunization-against-the-highly-pathogenic-prrsv-infection
#20
Chaitawat Sirisereewan, Teerawut Nedumpun, Sawang Kesdangsakonwut, Yonlayong Woonwong, Roongtham Kedkovid, Jirapat Arunorat, Roongroje Thanawongnuwech, Sanipa Suradhat
Porcine reproductive and respiratory syndrome virus (PRRSV) infection is one of the most important swine pathogens, and causes a major economic impact worldwide. Recently, a new variant type 2 PRRSV, highly pathogenic PRRSV (HP-PRRSV) has emerged and continued to circulate in Southeast Asia region. Currently, commercially available PRRSV vaccines, modified live PRRS vaccines (MLV) are not able to provide complete protection against HP-PRRSV and been reported to induce negative immunomodulatory effects. Interestingly, a novel DNA vaccine was developed and successfully used to improve PRRSV-specific immune responses following MLV vaccination...
January 2017: Veterinary Immunology and Immunopathology
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