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https://www.readbyqxmd.com/read/28820376/influenza-a-h3n2-virus-in-swine-at-agricultural-fairs-and-transmission-to-humans-michigan-and-ohio-usa-2016
#1
Andrew S Bowman, Rasna R Walia, Jacqueline M Nolting, Amy L Vincent, Mary Lea Killian, Michele M Zentkovich, Joshua N Lorbach, Sarah E Lauterbach, Tavis K Anderson, C Todd Davis, Natosha Zanders, Joyce Jones, Yunho Jang, Brian Lynch, Marisela R Rodriguez, Lenee Blanton, Stephen E Lindstrom, David E Wentworth, John Schiltz, James J Averill, Tony Forshey
In 2016, a total of 18 human infections with influenza A(H3N2) virus occurred after exposure to influenza-infected swine at 7 agricultural fairs. Sixteen of these cases were the result of infection by a reassorted virus with increasing prevalence among US swine containing a hemagglutinin gene from 2010-11 human seasonal H3N2 strains.
September 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28819273/enhanced-anaerobically-digested-swine-wastewater-treatment-by-the-composite-of-polyaluminum-chloride-pac-and-bacillus-megatherium-g106-derived-eps
#2
Junyuan Guo, Yang Huang, Cheng Chen, Yu Xiao, Jing Chen, Biyu Jian
A strain was isolated from biological sludge to produce EPS by using anaerobically digested swine wastewater (ADSW). Potential of the EPS in ADSW treatment were discussed. Results showed that the optimal fermentation medium for EPS production was determined as 4 g K2HPO4, 2 g KH2PO4, and 2 g sucrose dissolved in 1 L ADSW. After fermentation for 60 h, 2.98 g EPS with main backbone of polysaccharides can be extracted from 1 L of fermentation broth. The EPS showed good performances in ADSW treatment, after conditioned by this EPS, removal efficiencies of COD, ammonia, and TP reached 70...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28818749/a-conjugate-protein-containing-hiv-tat-isg20-and-a-prrsv-polymerase-binding-inhibits-prrsv-replication-and-may-be-a-novel-therapeutic-platform
#3
Ke Liu, Yuming Li, Bin Zhou, Feifei Wang, Beili Huan, Donghua Shao, Jianchao Wei, Yafeng Qiu, Beibei Li, Yingjuan Qian, Yong-Sam Jung, Denian Miao, Guangzhi Tong, Zhiyong Ma
Porcine Reproductive and Respiratory Syndrome (PRRS), which is caused by Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infection, has caused substantial economic losses for the global swine industry. To date, there are limited commercially available measures to control the spread of PRRSV. The available vaccines are unstable and there is no anti-PRRSV therapeutic available. Therefore, this study designed a novel recombinant antiviral protein that included a novel polypeptide that binds to the PRRSV polymerase (p9), the transduction ability of the HIV TAT, and the nucleotide degradation activity of interferon stimulated gene 20 (ISG20)...
August 10, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28817508/end-tidal-co2-guided-chest-compression-delivery-improves-survival-in-a-neonatal-asphyxial-cardiac-arrest-model
#4
Justin T Hamrick, Jennifer L Hamrick, Utpal Bhalala, Jillian S Armstrong, Jeong-Hoo Lee, Ewa Kulikowicz, Jennifer K Lee, Sapna R Kudchadkar, Raymond C Koehler, Elizabeth A Hunt, Donald H Shaffner
OBJECTIVES: To determine whether end-tidal CO2-guided chest compression delivery improves survival over standard cardiopulmonary resuscitation after prolonged asphyxial arrest. DESIGN: Preclinical randomized controlled study. SETTING: University animal research laboratory. SUBJECTS: 1-2-week-old swine. INTERVENTIONS: After undergoing a 20-minute asphyxial arrest, animals received either standard or end-tidal CO2-guided cardiopulmonary resuscitation...
August 16, 2017: Pediatric Critical Care Medicine
https://www.readbyqxmd.com/read/28817375/effect-of-maternal-antibody-transfer-on-antibody-dynamics-and-control-of-porcine-circovirus-type-2-infection-in-offspring
#5
Cheryl M T Dvorak, Brian J Payne, Jessica L Seate, Michael P Murtaugh
Sow immunity plays an important role in preventing viral infection and disease in newborn piglets. Vertical transmission of porcine circovirus type 2 (PCV2) may perpetuate porcine circovirus associated disease (PCVAD) in newborn and growing pigs. Hence, the immunological effects of maternal immunoglobulin transfer of PCV2-specific antibodies on PCV2 viremia and immune response in piglets in commercial swine herds were evaluated. Sow vaccination has been shown to reduce viral shedding and viremia, and increases the neutralizing antibody (NA) titers...
August 17, 2017: Viral Immunology
https://www.readbyqxmd.com/read/28814774/molecular-characterization-of-two-novel-echovirus-18-recombinants-associated-with-hand-foot-mouth-disease
#6
Haihao Zhang, Yilin Zhao, Hongbo Liu, Hao Sun, Xiaoqin Huang, Zhaoqing Yang, Shaohui Ma
Human echovirus 18 (E-18) is a member of the enterovirus B species. To date, sixteen full-length genome sequences of E-18 are available in the GenBank database. In this study, we describe the complete genomic characterization of two E-18 strains isolated in Yunnan, China. Pairwise comparisons of the nucleotide sequences and the deduced amino acid sequences revealed that the two Yunnan E-18 strains had 87.5% nucleotide identity and 96.3-96.5% amino acid identity with the Chinese strain. Phylogenetic and bootscanning analyses revealed the two E-18 strains had the highest identity with other several EV-B serotypes than the other E-18 strains in the P3 coding region, especially, 3B region of the Swine Vesicular disease virus (SVDV) strain HK70, indicated that frequent intertypic recombination might have occurred in the two Yunnan strains...
August 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28814518/a-single-amino-acid-substitution-at-position-225-in-ha-alters-the-transmissibility-of-eurasian-avian-like-h1n1-swine-influenza-virus-in-guinea-pigs
#7
Zeng Wang, Huanliang Yang, Yan Chen, Shiyu Tao, Liling Liu, Huihui Kong, Shujie Ma, Fei Meng, Yasuo Suzuki, Chuanling Qiao, Hualan Chen
Efficient transmission from human to human is the prerequisite for an influenza virus to cause a pandemic; however, the molecular determinants of influenza virus transmission are still largely unknown. In this study, we explored the molecular basis for transmission of Eurasian avian-like H1N1 (EAH1N1) swine influenza viruses by comparing two viruses that are genetically similar but differ in their transmissibility in guinea pigs: the A/swine/Guangxi/18/2011 virus (GX/18) is highly transmissible by respiratory droplet in guinea pigs, whereas the A/swine/Heilongjiang/27/2012 virus (HLJ/27) does not transmit in this animal model...
August 16, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28814514/ba71%C3%AE-cd2-a-new-recombinant-live-attenuated-african-swine-fever-virus-with-cross-protective-capabilities
#8
Paula L Monteagudo, Anna Lacasta, Elisabeth López, Laia Bosch, Javier Collado, Sonia Pina-Pedrero, Florencia Correa-Fiz, Francesc Accensi, María Jesús Navas, Enric Vidal, María J Bustos, Javier M Rodríguez, Andreas Gallei, Veljko Nikolin, María L Salas, Fernando Rodríguez
African swine fever is a highly contagious viral disease of mandatory declaration to the World Organization for Animal Health (OIE). Lack of available vaccines make its control difficult and thus ASFV represents a major threat to the swine industry. Inactivated vaccines do not confer solid protection against African swine fever virus (ASFV). Conversely, live attenuated viruses (LAV), either naturally isolated or obtained by genetic manipulation, have demonstrated reliable protection against homologous ASFV strains, albeit little or no protection has been demonstrated against heterologous viruses...
August 16, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28813691/effects-of-superabsorbent-polymers-on-the-abundances-of-antibiotic-resistance-genes-mobile-genetic-elements-and-the-bacterial-community-during-swine-manure-composting
#9
Aiyun Guo, Jie Gu, Xiaojuan Wang, Ranran Zhang, Yanan Yin, Wei Sun, Xiaxia Tuo, Li Zhang
Superabsorbent polymers (SAPs) are considered suitable amendments for reducing the selection pressure due to heavy metals and the abundances of antibiotic resistance genes (ARGs) during composting. In this study, three SAP (sodium polyacrylate) levels (0, 5, and 15mgkg(-1) of compost) were applied and their effects on the abundances of ARGs, mobile genetic elements (MGEs), and the bacterial community were investigated. After composting, the abundances of ARGs and MGEs decreased to different extent, where the removal efficiencies for tetW, dfrA7, ermX, aac(6')-ib-cr and MGEs exceeded 90%...
August 5, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28813611/intrauterine-growth-restricted-yucatan-miniature-pigs-experience-early-catch-up-growth-leading-to-greater-adiposity-and-impaired-lipid-metabolism-as-young-adults
#10
Semone B Myrie, Leslie L McKnight, J Christopher King, John J McGuire, Bruce N Van Vliet, Sukhinder K Cheema, Robert F Bertolo
Early nutrition has critical influences on cardiovascular disease risk in adulthood. The study objectives were to evaluate the impact of low birth weight on fasting and postprandial lipid metabolism and endothelium function in Yucatan miniature pigs. Intrauterine growth-restricted (IUGR) piglets (n = 6, 3 d old, 0.73 ± 0.04 kg) were paired with normal weight (NW) same-sex littermates (n = 6, 1.11 ± 0.05 kg) and fed milk replacer ad libitum for 4 wk. Thereafter, all pigs were fed a standard diet ad libitum for 5 h/d with growth, intakes and blood samples collected for 8 mo...
August 16, 2017: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/28811186/identification-and-characterization-of-small-molecule-inhibitors-of-porcine-reproductive-and-respiratory-syndrome-virus
#11
Alyssa B Evans, Pengfei Dong, Hyelee Loyd, Jianqiang Zhang, George A Kraus, Susan Carpenter
Porcine reproductive and respiratory syndrome virus (PRRSV) is the etiological agent of PRRS, an economically significant disease of swine worldwide. PRRSV is poorly controlled by the currently available vaccines, and alternative control strategies are needed to help prevent the continual circulation of the virus. Previously, we developed a synthetic route for the natural compound atractylodinol and demonstrated anti-PRRSV activity in vitro. However, the synthetic route was inefficient and the yield was poor...
August 12, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28810835/secondary-organizing-pneumonia-following-viral-pneumonia-caused-by-severe-influenza-b-a-case-report-and-literature-reviews
#12
Nobuhiro Asai, Toyoharu Yokoi, Naoya Nishiyama, Yusuke Koizumi, Daisuke Sakanashi, Hideo Kato, Mao Hagihara, Hiroyuki Suematsu, Yuka Yamagishi, Hiroshige Mikamo
BACKGROUND: Some reported that organizing pneumonia (OP) may occur after influenza A infections including swine-origin influenza A (H1N1). However, OP associated with influenza B infection has never been reported. We report the first case of secondary OP associated with viral pneumonia caused by influenza B. CASE PRESENTATION: A 23-year old woman was diagnosed as viral pneumonia caused by type B influenza. Despite of antiviral therapy, abnormal chest shadows were not improved...
August 15, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28807552/assessment-of-deformation-of-the-mitral-valve-complex-during-off-pump-coronary-artery-bypass-surgery-using-three-dimensional-echocardiography-in-a-porcine-model
#13
Takashi Igarashi, Masumi Iwai-Takano, Hiroki Wakamatsu, Mineyuki Haruta, Sadao Omata, Hitoshi Yokoyama
BACKGROUND: This study aimed to assess the deformation of the mitral valve complex during the displacement of the beating heart by using three-dimensional echocardiography in a porcine off-pump coronary artery bypass grafting (OPCAB) model. METHODS: In nine healthy swine, we positioned the beating heart as an OPCAB model, i.e. control, left anterior descending artery (LAD), right coronary artery (RCA), and left circumflex artery (LCX) positions. In each position, three-dimensional echocardiography was performed to assess the mitral valve complex with hemodynamic parameters...
August 11, 2017: Journal of Cardiology
https://www.readbyqxmd.com/read/28807279/acaricidal-activity-of-fluralaner-against-ornithodoros-moubata-and-ornithodoros-erraticus-argasid-ticks-evaluated-through-in-vitro-feeding
#14
R Pérez-Sánchez, A Oleaga
Ornithodoros erraticus and Ornithodoros moubata are argasid tick vectors that transmit severe diseases such as African swine fever and human relapsing fever. Elimination of the synanthropic populations of these vectors would facilitate the control of these diseases. Fluralaner is a novel isoxazoline that selectively blocks the GABA- and glutamate-gated channels, providing potent insecticidal and acaricidal activity. The aim of the current study was to provide quantitative data on the susceptibility of males, females and third nymphal instar of O...
August 30, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28806673/preservation-and-reactivation-of-candidatus-jettenia-asiatica-and-anammoxoglobus-propionicus-using-different-preservative-agents
#15
A Viancelli, M C Pra, L A Scussiato, M Cantão, A M G Ibelli, A Kunz
Anaerobic ammonium oxidation (anammox) bacteria have peculiar characteristics that make them difficult to cultivate. The conservation of these microorganisms in culture collections or laboratories requires successful preservation and reactivation techniques. Furthermore, studies have shown that successful reactivation may be preservative dependent. Considering this, the present study aimed to evaluate the preservation and reactivation of anammox consortia enriched from swine manure treatment lagoons, by using different preservative agents at different temperatures: KNO3 (at 4 °C), glycerol (-20 °C, -80 °C), and skimmed cow milk (-20 °C, -80 °C, -200 °C)...
July 12, 2017: Chemosphere
https://www.readbyqxmd.com/read/28805927/the-relationship-between-different-measures-of-feed-efficiency-and-feeding-behavior-traits-in-duroc-pigs
#16
D Lu, S Jiao, F Tiezzi, M Knauer, Y Huang, K A Gray, C Maltecca
Utilization of feed in livestock species consists of a wide range of biological processes, and therefore, its efficiency can be expressed in various ways, including direct measurement, such as daily feed intake, as well as indicator measures, such as feeding behavior. Measuring feed efficiency is important to the swine industry, and its accuracy can be enhanced by using automated feeding systems, which record feed intake and associated feeding behavior of individual animals. Each automated feeder space is often shared among several pigs and therefore raises concerns about social interactions among pen mates with regard to feeding behavior...
August 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28805917/technical-note-impact-of-pedigree-depth-on-convergence-of-single-step-genomic-blup-in-a-purebred-swine-population
#17
I Pocrnic, D A L Lourenco, H L Bradford, C Y Chen, I Misztal
In genomic evaluations, it is desirable to have low computing cost while retaining high accuracy of evaluation for young animals. When the population is large but only few animals have phenotypes, especially for low heritability traits, the convergence rate of BLUP or single-step genomic BLUP (ssGBLUP) can be very slow. This study investigates the effect of pedigree truncation on convergence rate and solutions of ssGBLUP for data exhibiting slow convergence. The data consisted of 216,000, 221,000, 732,000, and 579,000 phenotypes on 4 traits...
August 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28805733/first-detection-of-rotavirus-group-c-in-asymptomatic-pigs-of-smallholder-farms-in-east-africa
#18
Joshua Oluoch Amimo, Eunice Magoma Machuka, Edward Okoth
Abstract: Group C rotavirus (RVC) has been described to be a causative agent of gastroenteritis in humans and animals including pigs, cows, and dogs. Fecal samples collected from asymptomatic pigs in smallholder swine farms in Kenya and Uganda were screened for the presence of group C rotaviruses (RVC) using a reverse transcription-polymerase chain reaction assay. A total of 446 samples were tested and 37 were positive (8.3%). A significantly larger (p < 0.05) number of RVC-positive samples was detected in groups of older pigs (5-6 months) than in younger piglets (1-2 months)...
August 14, 2017: Pathogens
https://www.readbyqxmd.com/read/28803100/optimization-of-aqueous-ammonia-soaking-of-manure-fibers-by-response-surface-methodology-for-unlocking-the-methane-potential-of-swine-manure
#19
Anna Lymperatou, Hariklia N Gavala, Ioannis V Skiadas
Swine manure mono-digestion often results to economically non-feasible processes, due to the high dilution and ammonia concentration together with the low degradation rates it presents. The effects of different parameters of Aqueous Ammonia Soaking (AAS) as a pretreatment for improving the digestion of manure fibers when coupled to an ammonia removal step were investigated in this study. Response Surface Methodology was followed and the influence and interactions of the following AAS parameters were studied: NH3 concentration, duration and solid-to-liquid ratio...
July 27, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28802752/in-vitro-and-in-vivo-studies-of-deglycosylated-chimeric-porcine-reproductive-and-respiratory-syndrome-virus-as-a-vaccine-candidate-and-its-realistic-revenue-impact-at-commercial-pig-production-level
#20
Jung-Ju Kim, Jung-Ah Lee, Hwi-Yeon Choi, Jang-Hyuck Han, Won Huh, Jae-Ho Pi, Jung-Keun Lee, Sangshin Park, Ki-Hyun Cho, Joong-Bok Lee
Porcine reproductive and respiratory syndrome virus (PRRSV) causes major economic losses in the swine industry worldwide. Vaccination is the most effective method to control the disease. In a previous study, a chimeric PRRSV named as K418 which had a genome composed of ORF 1 from the FL12 strain and ORF 2-7 from the Korean representative LMY strain was created. We constructed K418DM, K418 with deglycosylated glycoprotein 5 (GP5), to improve its humoral immunity. In the follow-up on in vivo and in vitro virological and serological tests, no back mutation in amino acids of GP5 associated with deglycosylation was shown after 9 passages on MARC-145 cells, whereas only one case of back mutation was detected after single passage in pig...
August 9, 2017: Vaccine
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