Venkataramanan Subramanian, Samuel J Farmer, Kelsey L Heiland, Kyle T Moore, Todd A Vander Wall, Weiman Sun, Yogesh B Chaudhari, Michael E Himmel, Stephen R Decker
Historically, heterologous protein production has been challenging using the hyper-cellulolytic fungus, Trichoderma reesei. T. reesei is known for poor transformation efficiency, low homologous recombination frequency, and marginal screening systems for identification of successful transformants. In this work, we have applied the 2A-peptide multi-gene expression system to co-express four enzymes, which include three cellulases, a cellobiohydrolase (CBH1), an endoglucanase (EG1), and a β-D-glucosidase (BGL1); as well as the enhanced Green Fluorescent Protein, (eGFP), used here as a marker for monitoring expression levels...
December 13, 2022: Journal of Industrial Microbiology & Biotechnology