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5-aminolevulinic acid

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https://www.readbyqxmd.com/read/29327259/5-aminolevulinic-acid-squalene-nanoassemblies-for-tumor-photodetection-and-therapy-in-vitro-studies
#1
Andrej Babič, V Herceg, E Bastien, H-P Lassalle, L Bezdetnaya, Norbert Lange
Protoporphyrin IX (PpIX) as natural photosensitizer derived from administration of 5-aminolevulinic acid (5-ALA) has found clinical use for photodiagnosis and photodynamic therapy of several cancers. However, broader use of 5-ALA in oncology is hampered by its charge and polarity that result in its reduced capacity for passing biological barriers and reaching the tumor tissue. Advanced drug delivery platforms are needed to improve the biodistribution of 5-ALA. Here, we report a new approach for the delivery of 5-ALA...
January 11, 2018: Nanoscale Research Letters
https://www.readbyqxmd.com/read/29309850/5-aminolevulinic-acid-mediated-photodynamic-therapy-inhibits-survival-activity-and-promotes-apoptosis-of-a375-and-a431-cells
#2
Jingjing Cai, Qiuping Zheng, Huifang Huang, Buhong Li
OBJECTIVES: The purpose of this study was to investigate the effects of 5-aminolaevulinic acid mediated photodynamic therapy (ALA-PDT) on the survival activity and apoptosis of human melanoma cell line A375 and non-melanoma skin carcinoma cell line A431 cells. The mechanism for cellular apoptosis was explored. METHODS: The cell survival activity was determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and the proportion of apoptotic cells was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining...
January 5, 2018: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29309849/comparison-of-treatment-of-basal-cell-carcinoma-between-surgery-and-intralesional-photodynamic-therapy-a-cross-sectional-study
#3
M J Suárez Valladares, J Vega, M A Rodríguez Prieto
BACKGROUND: Surgery is the treatment of choice in basal cell carcinoma (BCC), but new less invasive techniques are in development as photodynamic therapy. The main problem of this technique is the limited penetration. The use of an intralesional photosensitizer plus an irradiation with a 630 nm laser increased this penetration. OBJETIVES: To compare the effectiveness in treatment of BCC between surgery and intralesional photodynamic therapy (I-PDT). To identify the clearance rate differences between intralesional or external irradiation in I-PTD group...
January 5, 2018: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29305683/the-use-of-5-aminolevulinic-acid-to-assist-gross-total-resection-of-pediatric-astroblastoma
#4
Yuji Agawa, Takafumi Wataya
PURPOSE: Astroblastoma is an uncommon pediatric neuroepithelial tumor. The prognosis and appropriate treatment of astroblastoma were not well understood. Previous reports suggested the best treatment for astroblastoma is surgical total resection. The authors report a case of pediatric astroblastoma that underwent gross total resection with the use of fluorescent guidance by 5-aminolevulinic acid (5-ALA). CASE REPORT: A 13-year-old girl presented with the tumor that was well-circumscribed cystic and solid mass with marked gadolinium enhancement in the right occipital lobe...
January 5, 2018: Child's Nervous System: ChNS: Official Journal of the International Society for Pediatric Neurosurgery
https://www.readbyqxmd.com/read/29259740/radiochemical-synthesis-and-evaluation-of-13n-labeled-5-aminolevulinic-acid-for-pet-imaging-of-gliomas
#5
Adam B Pippin, Ronald J Voll, Yuancheng Li, Hui Wu, Hui Mao, Mark M Goodman
The endogenous amino acid, 5-aminolevulinic acid (5-ALA), has received significant attention as an imaging agent, including ongoing clinical trials for image-guided tumor resection due to its selective uptake and subsequent accumulation of the fluorescent protoporphyrin IX in tumor cells. Based on the widely reported selectivity of 5-ALA, a new positron emission tomography imaging probe was developed by reacting methyl 5-bromolevulinate with [13N] ammonia. The radiotracer, [13N] 5-ALA, was produced in high radiochemical yield (65%) in 10 min and could be purified using only solid phase cartridges...
December 14, 2017: ACS Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29258952/5-aminolevulinic-acid-photodynamic-therapy-in-refractory-vulvar-lichen-sclerosus-et-atrophicus-series-of-ten-cases
#6
Ting Lan, Yongzhen Zou, Michael R Hamblin, Rui Yin
BACKGROUND: Vulvar lichen sclerosus et atrophicus (VLSA) is a chronic inflammatory skin disease of unknown etiology that mainly affects postmenopausal and perimenopausal women. The primary clinical symptoms of VLSA are itching, burning pain, and dyspareunia that can results in decreased quality of life. Existing therapies including topical corticosteroid ointment, topical calcineurin inhibitors, estrogens, are not very effective for treatment of VLSA. OBJECTIVE: To evaluate the effectiveness and safety of 5-aminolevulinic acid mediated photodynamic therapy (ALA-PDT) in the treatment of VLSA...
December 16, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29247361/biocompatible-5-aminolevulinic-acid-au-nanoparticle-loaded-ethosomal-vesicles-for-in-vitro-transdermal-synergistic-photodynamic-photothermal-therapy-of-hypertrophic-scars
#7
Zheng Zhang, Yunsheng Chen, Jiayue Ding, Chunlei Zhang, Amin Zhang, Dannong He, Yixin Zhang
Biocompatible 5-aminolevulinic acid/Au nanoparticle-loaded ethosomal vesicle (A/A-ES) is prepared via ultrasonication for synergistic transdermal photodynamic/photothermal therapy (PDT/PTT) of hypertrophic scar (HS). Utilizing ultrasonication, Au nanoparticles (AuNPs) are synthesized and simultaneously loaded in ethosomal vesicles (ES) without any toxic agents, and 5-aminolevulinic acid (ALA) is also loaded in ES with 20% of the entrapment efficiency (EE). The prepared A/A-ES displays strong absorbance in 600-650 nm due to the plasmonic coupling effect between neighboring AuNPs in the same A/A-ES, which can simultaneously stimulate A/A-ES to produce heat and enhance quantum yields of reactive oxygen species (ROS) by using 632 nm laser...
December 15, 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/29227548/resistance-of-oral-cancer-cells-to-5-ala-mediated-photodynamic-therapy
#8
Flávia Cristina Perillo Rosin, Marina Gabriela Teixeira, Cibele Pelissari, Luciana Corrêa
Photodynamic therapy (PDT) has been indicated for oral squamous cell carcinoma (OSCC) at early stages. Chemo and radio-resistance are frequently observed in OSCC, but it is unknown whether this tumor can develop resistance to PDT. It was investigated the process of acquiring resistance to multiple cycles of PDT by using OSCC cells. We also analyzed the expression of anti-apoptotic proteins and those related to Akt/mTOR pathway. Sub-lethal doses of PDT were applied, consisting of a constant concentration of 5-aminolevulinic acid (5-ALA) (1 mM, 4-hour incubation) and increasing irradiation dose with LED (from 5...
December 11, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29227199/two-step-irradiance-treatment-can-achieve-excellent-pain-control-during-red-light-5-aminolevulinic-acid-photodynamic-therapy-for-actinic-keratoses
#9
Gyorgy Paragh, Nathalie C Zeitouni
OBJECTIVE: To evaluate the ability of two-step irradiance to maintain pain control during red light 5-aminolevulinic acid (ALA) photodynamic therapy (PDT) for actinic keratoses (AK) and assess factors influencing pain. BACKGROUND: PDT provides excellent clinical and cosmetic results in the treatment AK and early basal cell carcinomas (BCC). Widespread use of PDT is limited, in part, by pain. A two-step irradiance method for PDT has previously been shown to significantly reduce PDT-associated pain during the treatment of BCC, but the ability of this method to limit pain during the treatment of AKs has not been reported...
December 11, 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/29222482/poly-methyl-vinyl-ether-alt-maleic-acid-and-ethyl-monoester-as-building-polymers-for-drug-loadable-electrospun-nanofibers
#10
Amalia Mira, C Reyes Mateo, Ricardo Mallavia, Alberto Falco
New biomaterials are sought for the development of bioengineered nanostructures. In the present study, electrospun nanofibers have been synthesized by using poly(methyl vinyl ether-alt-maleic acid) and poly(methyl vinyl ether-alt-maleic ethyl monoester) (PMVEMA-Ac and PMVEMA-ES, respectively) as building polymers for the first time. To further functionalize these materials, nanofibers of PMVEMA-Ac and PMVEMA-ES containing a conjugated polyelectrolyte (HTMA-PFP, blue emitter, and HTMA-PFNT, red emitter) were achieved with both forms maintaining a high solid state fluorescence yield without altered morphology...
December 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29196245/novel-potential-photodynamic-therapy-strategy-using-5-aminolevulinic-acid-for-ovarian-clear-cell-carcinoma
#11
Toshiya Teshigawara, Mika Mizuno, Takuya Ishii, Yuya Kitajima, Fumi Utsumi, Jun Sakata, Hiroaki Kajiyama, Kiyosumi Shibata, Masahiro Ishizuka, Fumitaka Kikkawa
BACKGROUND: Photodynamic therapy (PDT) is known as a minimally invasive treatment for cancer. 5-Aminolevulinic acid (ALA) is a precursor of the photosensitizing agent protoporphyrin IX (PpIX). Patients with ovarian clear-cell carcinoma (CCC) have poorer prognoses than those of patients with other histological CCC types. We evaluated the efficacy of ALA-PDT on CCC cells in vitro. METHODS: We used seven human CCC cell lines to measure the cytotoxicity of ALA-PDT. PpIX production in cancer cells was measured using a micro-plate reader...
November 28, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29194091/menthol-reduces-phototoxicity-pain-in-a-mouse-model-of-photodynamic-therapy
#12
Lisa Wright, Daniel Baptista-Hon, Fiona Bull, Faith Dalgaty, Michael Gallacher, Sally H Ibbotson, Tim G Hales
Phototoxicity-induced pain is a major clinical problem triggered by light acting on photosensitising drugs or endogenous porphyrins, notably protoporphyrin IX (PpIX), an intermediary in heme biosynthesis. Protoporphyrin IX accumulates in individuals with erythropoietic protoporphyria and is elevated during photodynamic therapy subsequent to application of 5-aminolevulinic acid (ALA). Pain occurs during irradiation of PpIX and responds poorly to conventional analgesics. Our objective was to develop a model of PpIX phototoxicity pain and investigate the potential of menthol as an analgesic...
November 25, 2017: Pain
https://www.readbyqxmd.com/read/29188101/photodynamic-opening-of-blood-brain-barrier
#13
Oxana Semyachkina-Glushkovskaya, Jürgen Kurths, Ekaterina Borisova, Sergei Sokolovski, Vanya Mantareva, Ivan Angelov, Alexander Shirokov, Nikita Navolokin, Natalia Shushunova, Alexander Khorovodov, Maria Ulanova, Madina Sagatova, Ilana Agranivich, Olga Sindeeva, Artem Gekalyuk, Anastasiya Bodrova, Edik Rafailov
Photodynamic treatment (PDT) causes a significant increase in the permeability of the blood-brain barrier (BBB) in healthy mice. Using different doses of laser radiation (635 nm, 10-40 J/cm2) and photosensitizer (5-aminolevulinic acid - 5-ALA, 20 and 80 mg/kg, i.v.), we found that the optimal PDT for the reversible opening of the BBB is 15 J/cm2 and 5-ALA, 20 mg/kg, exhibiting brain tissues recovery 3 days after PDT. Further increases in the laser radiation or 5-ALA doses have no amplifying effect on the BBB permeability, but are associated with severe damage of brain tissues...
November 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/29164655/role-of-two-5-aminolevulinic-acid-biosynthetic-pathways-in-heme-and-secondary-metabolite-biosynthesis-in-amycolatopsis-orientalis
#14
Li Zhu, Xiuping Qian, Daijie Chen, Mei Ge
Analysis of the Amycolatopsis orientalis genome revealed that two genes, hemA1 and hemA2, belonging to divergent pathways, were involved in the biosynthesis of 5-aminolevulinic acid. The roles of hemA1 and hemA2 were elucidated via genetic manipulation and metabolite analysis. The disruption of hemA1, encoding the glutamyl-tRNA(Glu) reductase of the C5 pathway, was essential for cell growth and is used for heme synthesis. Overexpression of hemA1 resulted in elevated vancomycin and ECO-0501 production in Amycolatopsis orientalis, and it was also effective in increasing the production of daptomycin and natamycin in other Streptomycetes...
November 22, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/29156557/the-invasive-region-of-glioblastoma-defined-by-5ala-guided-surgery-has-an-altered-cancer-stem-cell-marker-profile-compared-to-central-tumour
#15
Stuart J Smith, Mohammed Diksin, Saachi Chhaya, Shwetha Sairam, Maria A Estevez-Cebrero, Ruman Rahman
Glioblastoma, a WHO grade IV astrocytoma, is a highly aggressive and heterogeneous tumour that infiltrates deeply into surrounding brain parenchyma, making complete surgical resection impossible. Despite chemo-radiotherapy, the residual cell population within brain parenchyma post-surgery causes inevitable recurrence. Previously, the tumour core has been the focus of research and the basis for targeted therapeutic regimes, which have failed to improve survival in clinical trials. Here, we focus on the invasive margin as defined by the region with 5-aminolevulinic acid (5ALA) (GliolanTM) fluorescence at surgery beyond the T1 enhancing region on magnetic resonance imaging (MRI)...
November 18, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29142297/5-aminolevulinic-acid-guided-sampling-of-glioblastoma-microenvironments-identifies-pro-survival-signaling-at-infiltrative-margins
#16
James L Ross, Lee A D Cooper, Jun Kong, David Gutman, Merete Williams, Carol Tucker-Burden, Myles R McCrary, Alexandros Bouras, Milota Kaluzova, William D Dunn, Duc Duong, Constantinos G Hadjipanayis, Daniel J Brat
Glioblastoma (GBM) contains diverse microenvironments with uneven distributions of oncogenic alterations and signaling networks. The diffusely infiltrative properties of GBM result in residual tumor at neurosurgical resection margins, representing the source of relapse in nearly all cases and suggesting that therapeutic efforts should be focused there. To identify signaling networks and potential druggable targets across tumor microenvironments (TMEs), we utilized 5-ALA fluorescence-guided neurosurgical resection and sampling, followed by proteomic analysis of specific TMEs...
November 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29139243/wide-field-spectrally-resolved-quantitative-fluorescence-imaging-system-toward-neurosurgical-guidance-in-glioma-resection
#17
Yijing Xie, Maria Thom, Michael Ebner, Victoria Wykes, Adrien Desjardins, Anna Miserocchi, Sebastien Ourselin, Andrew W McEvoy, Tom Vercauteren
In high-grade glioma surgery, tumor resection is often guided by intraoperative fluorescence imaging. 5-aminolevulinic acid-induced protoporphyrin IX (PpIX) provides fluorescent contrast between normal brain tissue and glioma tissue, thus achieving improved tumor delineation and prolonged patient survival compared with conventional white-light-guided resection. However, commercially available fluorescence imaging systems rely solely on visual assessment of fluorescence patterns by the surgeon, which makes the resection more subjective than necessary...
November 2017: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/29139095/local-alkylating-chemotherapy-applied-immediately-after-5-ala-guided-resection-of-glioblastoma-does-not-provide-additional-benefit
#18
William Sage, Mathew Guilfoyle, Catriona Luney, Adam Young, Rohitashwa Sinha, Donatella Sgubin, Joseph H McAbee, Ruichong Ma, Sarah Jefferies, Rajesh Jena, Fiona Harris, Kieren Allinson, Tomasz Matys, Wendi Qian, Thomas Santarius, Stephen Price, Colin Watts
Grade IV glioma is the most common and aggressive primary brain tumour. Gross total resection with 5-aminolevulinic acid (5-ALA) guided surgery combined with local chemotherapy (carmustine wafers) is an attractive treatment strategy in these patients. No previous studies have examined the benefit carmustine wafers in a treatment programme of 5-ALA guided resection followed by a temozolomide-based chemoradiotherapy protocol. The objective of this study was to examine the benefit of carmustine wafers on survival in patients undergoing 5-ALA guided resection...
November 14, 2017: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/29131243/photodynamic-therapy-with-5-aminolevulinic-acid-suppresses-ifn-%C3%AE-induced-k17-expression-in-hacat-cells-via-mapk-pathway
#19
X-L Wang, Q Sun
OBJECTIVE: Psoriasis is a chronic inflammatory skin disorder that greatly affects the patient's quality of life. Photodynamic therapy (PDT) with 5-aminolevulinic acid (ALA) has recently been applied for inflammatory dermatoses including psoriasis. However, the therapeutic effect of ALA-PDT is yet to be validated, and the underlying mechanisms remain unclear. MATERIALS AND METHODS: In this study, a psoriatic model was established by treating HaCaT cells with 250 U/ml IFN-γ for 48 h...
October 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29129543/idiopathic-elastosis-perforans-serpiginosa-with-satisfactory-response-after-5-ala-photodynamic-therapy
#20
S Alique-García, J Company-Quiroga, C Horcajada-Reales, B Echeverría-García, J C Tardío-Dovao, J Borbujo
Photodynamic therapy (PDT) involves the use of photochemical reactions mediated through the interaction of photosensitizing agents, light, and oxygen for the treatment of malignant or benign diseases. Topical photosensitizers employed in dermatology are 5-aminolevulinic acid (5 ALA) and methyl aminolevulinate, classically used for the treatment of superficial non-melanoma skin cancer and their precursors. Recently the efficacy of PDT has been introduced in other benign diseases. Elastosis perforans serpiginosa (EPS) is a rare skin disorder characterized by transepidermal elimination of abnormal elastic fibers...
November 9, 2017: Photodiagnosis and Photodynamic Therapy
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