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Protoporphyrin ix

Yanhong Lv, Jinhua Zheng, Qi Zhou, Limin Jia, Chunying Wang, Nian Liu, Hong Zhao, Hang Ji, Baoxin Li, Wenwu Cao
Sonodynamic therapy (SDT) is an innovative modality for cancer treatment. But the biological effect of SDT on oral squamous cell carcinoma has not been studied. Our previous study has shown that endo-Protoporphyrin IX based SDT (ALA-SDT) could induce apoptosis in human tongue squamous carcinoma SAS cells through mitochondrial pathway. Herein, we investigated the effect of exo- Protoporphyrin based SDT (PpIX-SDT) on SAS cells in vitro and in vivo. We demonstrated that PpIX-SDT increased the ratio of cells in the G2/M phase and induced 3-4 times more cell apoptosis compared to sonocation alone...
January 19, 2017: Scientific Reports
Zheng Huang, Songsheng Shi, Haixia Qiu, Desheng Li, Jian Zou, Shaoshan Hu
Surgical removal of tumor mass is a common approach in the management of brain tumors. However, the precise delineation of normal tissue from tumor tissue for a complete resection of tumor mass in brain tumor surgery remains a difficult task for neurosurgeons. Aminolevulinic acid (ALA)-mediated exgogenous fluorescence of protoporphyrin IX (PpIX) is a sensitive approach for tumor imaging. Recent studies suggest that the use of ALA/PpIX-mediated fluorescence-guided resection (FGR) or fluorescence-guided surgery can enable more accurate and complete resection of brain tumors, especially when used in quantitative fashion...
January 2017: Neurophotonics
Xuezheng Fan, Linshen Mu
OBJECTIVE: To investigate the role of heme oxygenase-1 (HO-1) in the regulation of inflammatory reaction, neuronal cell proliferation and apoptosis in rats after intracerebral hemorrhage (ICH). METHODS: Thirty-six adult Sprague Dawley (SD) male rats were randomly divided into sham operation, ICH and zinc protoporphyrin (ZPP) group. Rats (except for the sham operation group) were given 50 μL stereotactic injection of autologous blood from the femoral artery into the caudate nucleus, to establish an ICH model...
2017: Neuropsychiatric Disease and Treatment
Jasmin Barman-Aksözen, Paulina Ćwiek, Vijay B Bansode, Frank Koentgen, Judith Trüb, Pawel Pelczar, Paolo Cinelli, Xiaoye Schneider-Yin, Daniel Schümperli, Elisabeth I Minder
Erythropoietic Protoporphyria (EPP) is caused by deficiency of ferrochelatase (FECH) which incorporates iron into protoporphyrin IX (PPIX) to form heme. Excitation of accumulated PPIX by light generates oxygen radicals which evoke excessive pain and, after longer light exposure, ulcerations in exposed skin areas of EPP patients. Moreover, ∼5% of the patients develop a liver dysfunction due to PPIX accumulation. Most patients (∼97%) have a severe FECH mutation (Mut) in trans to an intronic polymorphism (c...
January 12, 2017: Disease Models & Mechanisms
Yoonsun Jang, Sojin Kim, Seungae Lee, Chang-Min Yoon, Inkyu Lee, Jyongsik Jang
Graphene oxide (GO) enwrapped SiO2/TiO2 hollow nanoparticles (GO-HNP) are synthesized by stöbber method and used as a nanocarrier for loading protoporphyrin IX (PpIX). The synthesized nanoparticle has high dispersibitliy and high uniformity in diameter (ca. 50 nm). . Furthermore, this nanoparticle shows 808 nm laser induced PpIX release property (photo-induced "on-off" drug release system). The GO-HNP-PpIX is employed for inducing both photothermal therapy (PTT) and photodynamic therapy (PDT). The synergic effect of the PTT and PDT exhibits powerful anti-cancer property...
January 16, 2017: Chemistry: a European Journal
Dexin Chen, Ran Tao, Ke Tao, Biqiong Chen, Seok Ki Choi, Qing Tian, Yawen Xu, Guangdong Zhou, Kang Sun
Singlet oxygen ((1) O2 ), as an important kind of reactive oxygen species (ROS) and main therapeutic agent in photodynamic therapy (PDT), only have a half-life of 40 ns and an effective radius of 20 nm, which cause significant obstacles for improving PDT efficacy. In this work, novel upconversion nanoparticle (UCN)-based nanoplatforms are developed with a minimized distance between UCNs and a photosensitizer, protoporphyrin IX (PpIX), and a controllable payload of PpIX, to enhance and control ROS production...
January 6, 2017: Small
J F Cornelius, J M Placke, J Knipps, I Fischer, M Kamp, H J Steiger
INTRODUCTION: Recently a mini-spectrometer with a handheld probe quantifying 5-aminolevulinic acid (5-ALA) based fluorescence intensity of brain tumors was developped by Kim and co-workers to improve fluorescence-guided neurosurgery. OBJECTIVE: To evaluate if this new tool is capable to discriminate nuances of fluorescence intensity of strongly fluorescing tumors (glioblastomas (GBM) and meningiomas (MM)). To study different modes of measurement (touch/no-touch)...
December 27, 2016: Photodiagnosis and Photodynamic Therapy
Kunihiro Ueta, Junkoh Yamamoto, Tohru Tanaka, Yoshiteru Nakano, Takehiro Kitagawa, Shigeru Nishizawa
5-Aminolevulinic acid (5-ALA) can accumulate protoporphyrin IX (PpIX) in tumour cell mitochondria and is well known for its utility in fluorescence-guided resection of malignant gliomas as a live molecular marker. Previously, we and other authors demonstrated that 5-ALA has a radiosensitizing effect for tumours. In the present study, we aimed to investigate the mechanism underlying the radiosensitizing effect of 5-ALA by focusing on glioma cell mitochondria. Using an enhancer (ciprofloxacin) of 5-ALA-induced PpIX accumulation, we evaluated the influence of ionizing irradiation (IR) and delayed reactive oxygen species (ROS) production 12 h after IR by colony-forming assay and flow cytometry (FCM) with different amounts of PpIX accumulation...
February 2017: International Journal of Molecular Medicine
Jun Qin, Yue He, Ming Duan, Meng Luo
OBJECTIVE: We explored the effects of Nuclear Factor-E2-related factor 2 (Nrf2) and Heme Oxygenase 1 (HO-1) on splanchnic hemodynamics in portal hypertensive rats. METHODS: Experimental cirrhosis with portal hypertension was induced by intraperitoneal injection of carbon tetrachloride. The expression of proteins was examined by immunoblotting. Hemodynamic studies were performed by radioactive microspheres. The vascular perfusion system was used to measure the contractile response of mesentery arterioles in rats...
December 23, 2016: Microvascular Research
Hatem Maamoun, Matshediso Zachariah, John H McVey, Fiona R Green, Abdelali Agouni
Most of diabetic cardiovascular complications are attributed to endothelial dysfunction and impaired angiogenesis. Endoplasmic Reticulum (ER) and oxidative stresses were shown to play a pivotal role in the development of endothelial dysfunction in diabetes. Hemeoxygenase-1 (HO-1) was shown to protect against oxidative stress in diabetes; however, its role in alleviating ER stress-induced endothelial dysfunction remains not fully elucidated. We aim here to test the protective role of HO-1 against high glucose-mediated ER stress and endothelial dysfunction and understand the underlying mechanisms with special emphasis on oxidative stress, inflammation and cell death...
December 21, 2016: Biochemical Pharmacology
De-Shui Yu, Yan-Song Wang, Yun-Long Bi, Zhan-Peng Guo, Ya-Jiang Yuan, Song-Ming Tong, Rui-Chao Su, Li-Hao Ge, Jian Wang, Ya-Li Pan, Ting-Ting Guan, Yang Cao
Salvianolic acid A (Sal A), a bioactive compound isolated from the Chinese medicinal herb Danshen, is used for the prevention and treatment of cardiovascular diseases. However, the protective function of Sal A on preserving the role of blood-spinal cord barrier (BSCB) after spinal cord injury (SCI) is unclear. The present study investigated the effects and mechanisms of Sal A (2.5, 5, 10 mg/kg, i.p.) on BSCB permeability at different time-points after compressive SCI in rats. Compared to the SCI group, treatment with Sal A decreased the content of the Evans blue in the spinal cord tissue at 24 h post-SCI...
December 20, 2016: Brain Research
Sarah Hollingshead, Sophie Bliss, Patrick J Baker, C Neil Hunter
Chlorophylls are modified tetrapyrrole molecules, essential for photosynthesis. These pigments possess an isocyclic E ring formed by the Mg-protoporphyrin IX monomethylester cyclase (MgPME-cyclase). We assessed the in vivo effects of altering seven highly conserved residues within Ycf54, which is required for MgPME-cyclase activity in the cyanobacterium Synechocystis Synechocystis strains harbouring the Ycf54 alterations D39A, F40A and R82A were blocked to varying degrees at the MgPME-cyclase step, whereas the A9G mutation reduced Ycf54 levels by ~75%...
December 22, 2016: Biochemical Journal
Guangqi Li, Congjiao Sun, Guiqin Wu, Fengying Shi, Aiqiao Liu, Ning Yang
Brown eggs are popular in many countries and consumers regard eggshell brownness as an important indicator of egg quality. However, the potential regulatory proteins and detailed molecular mechanisms regulating eggshell brownness have yet to be clearly defined. In the present study, we performed quantitative proteomics analysis with iTRAQ technology in the shell gland epithelium of hens laying dark and light brown eggs to investigate the candidate proteins and molecular mechanisms underlying variation in chicken eggshell brownness...
2016: PloS One
Xueyun Hu, Mike T Page, Akihiro Sumida, Ayumi Tanaka, Matthew J Terry, Ryouichi Tanaka
Proteins that contain iron-sulfur clusters play pivotal roles in various metabolic processes, such as photosynthesis and redox metabolism. Among the proteins involved in the biosynthesis of iron-sulfur clusters in plants, the SUFB subunit of the SUFBCD complex appears unique because SUFB has been reported to be involved in chlorophyll metabolism and phytochrome-mediated signaling. To gain insights into the function of the SUFB protein, we analyzed the phenotypes of two SUFB mutants, laf6 and hmc1, and RNAi lines with reduced SUFB expression...
December 22, 2016: Plant Journal: for Cell and Molecular Biology
Jaroslava Joniová, Emmanuel Gerelli, Matthieu Zellweger, Georges Wagnières
No abstract text is available yet for this article.
December 1, 2016: Journal of Biomedical Optics
Rinse Ubbink, Mark A Wefers Bettink, Rineke Janse, Floor A Harms, Tanja Johannes, F Michael Münker, Egbert G Mik
After introduction of the protoporphyrin IX-triplet state lifetime technique as a new method to measure mitochondrial oxygen tension in vivo, the development of a clinical monitor was started. This monitor is the "COMET", an acronym for Cellular Oxygen METabolism. The COMET is a non-invasive electrically powered optical device that allows measurements on the skin. The COMET is easy to transport, due to its lightweight and compact size. After 5-aminolevulinic acid application on the human skin, a biocompatible sensor enables detection of PpIX in the mitochondria...
December 20, 2016: Journal of Clinical Monitoring and Computing
Janine Kah, Tassilo Volz, Marc Lütgehetmann, Anne Groth, Ansgar W Lohse, Gisa Tiegs, Gabriele Sass, Maura Dandri
: Enhancement of host anti-oxidant enzymes, such as heme oxygenase-1, may attenuate virus-mediated hepatocyte injury, while the induction of HO-1 by cobalt-protoporphyrin-IX (CoPP) administration, as the application of its heme degradation product biliverdin (BV), was shown to hinder HCV replication in vitro. Additionally, (GT)n -repeats length in the polymorphic region of the HO-1 promoter may affect HO-1 expression and responsiveness to infection and disease severity. Aim of this study was to investigate the antiviral and hepatoprotective effects of CoPP-mediated HO-1 induction, alone or in combination with interferon alpha (peg-IFNα), in HCV-infected mice harboring hepatocytes from donors with different HO-1-promoter polymorphisms...
December 19, 2016: Liver International: Official Journal of the International Association for the Study of the Liver
Byung-Hak Kim, Kyoung Chan Park, Jeong Ho Park, Chung Gi Lee, Sang-Kyu Ye, Jung Youl Park
Abnormal accumulation of melanin pigments in the skin can be lead to hyperpigmentation disorders and melanoma. Melanin biosynthesis is ultimately regulated by the rate-limiting enzyme tyrosinase. In the present study, we synthesized chalcone derivatives and identified 1-(2-cyclohexylmethoxy-6-hydroxy-phenyl)-3-(4-hydroxymethyl-phenyl)-propenone (chalcone-21) as an anti-melanogenic substance in B16F10 melanoma cells. Chalcone-21 strongly inhibited cellular melanin production and tyrosinase activity in B16F10 melanoma cells stimulated with α-melanocyte stimulating hormone (α-MSH) or protoporphyrin IX...
October 27, 2016: Biochemical and Biophysical Research Communications
Amelie Wißbrock, Toni Kühl, Katja Silbermann, Albert J Becker, Oliver Ohlenschläger, Diana Imhof
Labile heme has been suggested to have an impact in several severe diseases. In the context of Alzheimer's disease (AD), however, decreased levels of free heme have been reported. Therefore, we were looking for an assay system that can be used for heme concentration determination. From a biochemical point of view the peroxidase activity of the Aβ-heme complex seemed quite attractive to pursue this goal. As a consequence, a peptide that is able to increase the readout even in the case of a low heme concentration is favorable...
December 15, 2016: Journal of Medicinal Chemistry
Ismael Galván, Pablo R Camarero, Rafael Mateo, Juan J Negro
Colours that underlie animal pigmentation can either be permanent or renewable in the short term. Here we describe the discovery of a conspicuous salmon-pink colouration in the base of bustard feathers and down that has never been reported because of its extraordinarily brief expression. HPLC analyses indicated that its constituent pigments are coproporphyrin III and protoporphyrin IX, which are prone to photodegradation. Accordingly, an experimental exposure of feathers of three bustard species to sunlight produced a rapid disappearance of the salmon-pink colouration, together with a marked decrease in reflectance around 670 nm coinciding with the absorption of porphyrin photoproducts...
December 15, 2016: Scientific Reports
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