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Fluorescence imaging in tumor mice

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https://www.readbyqxmd.com/read/28221751/molecular-engineering-and-design-of-semiconducting-polymer-dots-with-narrow-band-near-infrared-emission-for-in-vivo-biological-imaging
#1
Chi-Shiang Ke, Chia-Chia Fang, Jia-Ying Yan, Po-Jung Tseng, Joseph R Pyle, Chuan-Pin Chen, Shu-Yi Lin, Jixin Chen, Xuanjun Zhang, Yang-Hsiang Chan
This article describes the design and synthesis of donor-bridge-acceptor-based semiconducting polymer dots (Pdots) that exhibit narrow-band emissions, ultrahigh brightness, and large Stokes shifts in the near-infrared (NIR) region. We systematically investigated the effect of π-bridges on the fluorescence quantum yields of the donor-bridge-acceptor-based Pdots. The Pdots could be excited by a 488 or 532 nm laser and have a high fluorescence quantum yield of 33% with a Stokes shift of more than 200 nm. The emission full width at half-maximum of the Pdots can be as narrow as 29 nm, about 2...
February 23, 2017: ACS Nano
https://www.readbyqxmd.com/read/28213790/optical-see-through-cancer-vision-goggles-enable-direct-patient-visualization-and-real-time-fluorescence-guided-oncologic-surgery
#2
Suman B Mondal, Shengkui Gao, Nan Zhu, LeMoyne Habimana-Griffin, Walter J Akers, Rongguang Liang, Viktor Gruev, Julie Margenthaler, Samuel Achilefu
BACKGROUND: The inability to visualize the patient and surgical site directly, limits the use of current near infrared fluorescence-guided surgery systems for real-time sentinel lymph node biopsy and tumor margin assessment. METHODS: We evaluated an optical see-through goggle augmented imaging and navigation system (GAINS) for near-infrared, fluorescence-guided surgery. Tumor-bearing mice injected with a near infrared cancer-targeting agent underwent fluorescence-guided, tumor resection...
February 17, 2017: Annals of Surgical Oncology
https://www.readbyqxmd.com/read/28196364/technetium-99m-or-cy7-labeled-rituximab-as-an-imaging-agent-for-non-hodgkin-lymphoma
#3
Ximena Camacho, Camila Longo Machado, María Fernanda García, Juan Pablo Gambini, Agustina Banchero, Marcelo Fernández, Natalia Oddone, Daniela Bertolini Zanatta, Carolina Rosal, Carlos Alberto Buchpiguel, Roger Chammas, Eloisa Riva, Pablo Cabral
INTRODUCTION: Rituximab was the first monoclonal antibody approved for the treatment of B-cell non-Hodgkin lymphoma (NHL) expressing CD20 antigen. This antibody has also the potential to be used as a specific fluorescent and radiolabel agent for targeting NHL. OBJECTIVE: To radiolabel rituximab with technetium-99m (99mTc) or Cy7 and evaluate both probes as potential imaging agents for NHL. METHODS: Rituximab was derivatized with the trifluoroacetyl hydrazino protected form of succinimidyl ester of HYNIC and radiolabeled with 99mTc...
February 15, 2017: Oncology
https://www.readbyqxmd.com/read/28193013/highly-specific-and-ultrasensitive-two-photon-fluorescence-imaging-of-native-hocl-in-lysosomes-and-tissues-based-on-thiocarbamate-derivatives
#4
Baocun Zhu, Ping Li, Wei Shu, Xin Wang, Caiyun Liu, Yue Wang, Zuokai Wang, Yawei Wang, Bo Tang
Hypochlorous acid (HOCl) acts as a weak acid distributed mainly in acidic organelle lysosomes of phagocytes and plays a crucial role in the immune defense. The elaborate interrelation between the variations of HOCl levels in lysosomes and different physiological and pathological processes remains unclear. Thus, the accurate determination of lysosomal HOCl in living cells and in vivo is very important. Because of extremely low concentration and difficulty in distinguishing HOCl from OCl(-) under the physiological environment, it is still a great challenge to specifically monitor the intracellular intrinsic HOCl levels without exogenous stimulation, which impedes an exact understanding of its biological roles...
December 20, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/28179102/impact-of-surface-grafting-density-of-peg-macromolecules-on-dually-fluorescent-silica-nanoparticles-used-for-the-in-vivo-imaging-of-subcutaneous-tumors
#5
Laurent Adumeau, Coralie Genevois, Lydia Roudier, Christophe Schatz, Franck Couillaud, Stéphane Mornet
BACKGROUND: In the context of systematically administered nanomedicines, the physicochemistry of NP surfaces must be controlled as a prerequisite to improve blood circulation time, and passive and active targeting. In particular, there is a real need to develop NP stealth and labelling for both in vivo and microscopic fluorescence imaging in a mice model. METHODS: We have synthesized NIR/red dually fluorescent silica nanoparticles of 19nm covalently covered by a PEG layer of different grafting density in the brush conformational regime by using a reductive amination reaction...
February 4, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28179099/hydrophobically-modified-polysaccharide-based-on-polysialic-acid-nanoparticles-as-carriers-for-anticancer-drugs
#6
Bom Jung, Man-Kyu Shim, Min-Ju Park, Eun Hyang Jang, Hong Yeol Yoon, Kwangmeyung Kim, Jong-Ho Kim
This study presented the development of hydrophobically modified polysialic acid (HPSA) nanoparticles, a novel anticancer drug nanocarrier that increases therapeutic efficacy without causing nonspecific toxicity towards normal cells. HPSA nanoparticles were prepared by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS) coupling between N-deacetylated polysialic acid (PSA) and 5β-cholanic acid. The physicochemical characteristics of HPSA nanoparticles (zeta-potential, morphology and size) were measured, and in vitro cytotoxicity and cellular uptake of PSA and HPSA nanoparticles were tested in A549 cells...
February 4, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28157707/near-infrared-fluorescent-imaging-of-choline-kinase-alpha-expression-and-inhibition-in-breast-tumors
#7
Sean P Arlauckas, Manoj Kumar, Anatoliy V Popov, Harish Poptani, Edward J Delikatny
Choline kinase alpha (ChoKα) overexpression is associated with an aggressive tumor phenotype. ChoKα inhibitors induce apoptosis in tumors, however validation of their specificity is difficult in vivo. We report the use of optical imaging to assess ChoKα status in cells and in vivo using JAS239, a carbocyanine-based ChoKα inhibitor with inherent near infrared fluorescence. JAS239 attenuated choline phosphorylation and viability in a panel of human breast cancer cell lines. Antibody blockade prevented cellular retention of JAS239 indicating direct interaction with ChoKα independent of the choline transporters and catabolic choline pathways...
February 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28152672/intraoperative-localization-of-rectal-tumors-using-liposomal-indocyanine-green
#8
Shlomo Magdassi, Shoshi Bar-David, Yael Friedman-Levi, Ehud Zigmond, Chen Varol, Guy Lahat, Joseph Klausner, Sara Eyal, Eran Nizri
BACKGROUND: Tumor localization may pose a significant challenge during minimally invasive rectal resection. Near-infrared (NIR) imaging can penetrate biological tissue and afford tumor localization from the external surface of the rectum. Our aim was to develop an NIR-based tool for rectal tumor imaging that can be administered intravenously. METHODS: We prepared indocyanine-green (ICG)-loaded liposomes by sonication. Liposomes were evaluated for their size and morphology...
February 1, 2017: Surgical Innovation
https://www.readbyqxmd.com/read/28150209/fluorescence-and-bioluminescence-imaging-of-orthotopic-brain-tumors-in-mice
#9
Emilie McKinnon, Alfred Moore, Suraj Dixit, Yun Zhu, Ann-Marie Broome
Optical imaging strategies, such as fluorescence and bioluminescence imaging, are non-invasive, in vivo whole body imaging techniques utilized to study cancer. Optical imaging is widely used in preclinical work because of its ease of use and cost-friendliness. It also provides the opportunity to study animals and biological responses longitudinally over time. Important considerations include depth of tissue penetration, photon scattering, absorption and the choice of light emitting probe, all of which affect the resolution (image quality and data information) and the signal to noise ratio of the image...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28134273/imaging-tumor-microscopic-viscosity-in-vivo-using-molecular-rotors
#10
Lyubov' E Shimolina, Maria Angeles Izquierdo, Ismael López-Duarte, James A Bull, Marina V Shirmanova, Larisa G Klapshina, Elena V Zagaynova, Marina K Kuimova
The microscopic viscosity plays an essential role in cellular biophysics by controlling the rates of diffusion and bimolecular reactions within the cell interior. While several approaches have emerged that have allowed the measurement of viscosity and diffusion on a single cell level in vitro, the in vivo viscosity monitoring has not yet been realized. Here we report the use of fluorescent molecular rotors in combination with Fluorescence Lifetime Imaging Microscopy (FLIM) to image microscopic viscosity in vivo, both on a single cell level and in connecting tissues of subcutaneous tumors in mice...
January 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28126888/detection-of-micrometastases-using-spect-fluorescence-dual-modality-imaging-in-a-cea-expressing-tumor-model
#11
Marlène C H Hekman, Mark Rijpkema, Desirée Bos, Egbert Oosterwijk, David M Goldenberg, Peter F A Mulders, Otto C Boerman
BACKGROUND: Intraoperative dual-modality imaging can help the surgeon distinguish tumor from normal tissue. This technique may prove particularly valuable if small tumors need to be removed that are difficult to detect with the naked eye. The humanized anti-carcinoembryonic antigen (anti-CEA) monoclonal antibody, labetuzumab (hMN-14), can be used as a tumor targeting agent in colorectal cancer (CRC), since CEA is overexpressed in approximately 95% of CRC. Dual-labeled labetuzumab, labeled with both a near-infrared fluorescent dye (IRDye800CW) and a radioactive label (Indium-111), can be used as a tracer for dual-modality imaging...
January 26, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28117816/a-murine-orthotopic-bladder-tumor-model-and-tumor-detection-system
#12
Sin Mun Tham, Kesavan Esuvaranathan, Ratha Mahendran
This protocol describes the generation of bladder tumors in female C57BL/6J mice using the murine bladder cancer cell line MB49, which has been modified to secrete human Prostate Specific Antigen (PSA), and the procedure for the confirmation of tumor implantation. In brief, mice are anesthetized using injectable drugs and are made to lay in the dorsal position. Urine is vacated from the bladder and 50 µL of poly-L-lysine (PLL) is slowly instilled at a rate of 10 µL/20 s using a 24 G IV catheter. It is left in the bladder for 20 min by stoppering the catheter...
January 12, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28116362/a-rapid-albumin-binding-5-fluorouracil-prodrug-with-a-prolonged-circulation-time-and-enhanced-antitumor-activity
#13
Dongyang Zhao, Huicong Zhang, Wenhui Tao, Wei Wei, Jin Sun, Zhonggui He
5-Fluorouracil (5-FU) is an antimetabolite widely used in the treatment of a variety of solid tumors. However, its clinical applications are greatly hindered by a very short residence time in blood circulation and non-specific distribution in the body. In order to overcome these challenges, 1-alkylcarbonyloxymethyl 5-FU was designed and linked with a maleimide group to form an albumin-binding 5-FU prodrug, named EMC-5-FU. In vitro incubation with bovine serum albumin (BSA) and fresh rat blood proved that the prodrug bound rapidly to cysteine-34 to form the drug-albumin conjugate nanomedicine...
January 24, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28115248/nanoparticle-mediated-radiopharmaceutical-excited-fluorescence-molecular-imaging-allows-precise-image-guided-tumor-removal-surgery
#14
Zhenhua Hu, Chongwei Chi, Muhan Liu, Hongbo Guo, Zeyu Zhang, Chaoting Zeng, Jinzuo Ye, Jing Wang, Jie Tian, Weidong Yang, Wanhai Xu
Fluorescent molecular imaging technique has been actively explored for optical image-guided cancer surgery in pre-clinical and clinical research and has attracted many attentions. However, the efficacy of the fluorescent image-guided cancer surgery can be compromised by the low signal-to-noise ratio caused by the external light excitation. This study presents a novel nanoparticle-mediated radiopharmaceutical-excited fluorescent (REF) image-guided cancer surgery strategy, which employs the internal dual-excitation of europium oxide nanoparticles through both gamma rays and Cerenkov luminescence emitted from radiopharmaceuticals...
January 20, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28103010/a-fluorescent-hsp90-probe-demonstrates-the-unique-association-between-extracellular-hsp90-and-malignancy-in-vivo
#15
Lauren B Crowe, Philip F Hughes, David A Alcorta, Takuya Osada, Aaron P Smith, Juliane Totzke, David R Loiselle, Isaac D Lutz, Madhusudhana Gargesha, Debasish Roy, Jose Roques, David Darr, H Kim Lyerly, Neil L Spector, Timothy A J Haystead
Extracellular expression of heat shock protein 90 (eHsp90) by tumor cells is correlated with malignancy. Development of small molecule probes that can detect eHsp90 in vivo may therefore have utility in the early detection of malignancy. We synthesized a cell impermeable far-red fluorophore-tagged Hsp90 inhibitor to target eHsp90 in vivo. High resolution confocal and lattice light sheet microscopy show that probe-bound eHsp90 accumulates in punctate structures on the plasma membrane of breast tumor cells and is actively internalized...
February 23, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28094402/mesoporous-carbon-nanoshells-for-high-hydrophobic-drug-loading-multimodal-optical-imaging-controlled-drug-release-and-synergistic-therapy
#16
Hui Wang, Kui Wang, Qingxin Mu, Zachary R Stephen, Yanyan Yu, Shuiqin Zhou, Miqin Zhang
Loading and controlled release of sufficient hydrophobic drugs to tumor cells has been the bottleneck in chemotherapy for decades. Herein we report the development of a fluorescent and mesoporous carbon nanoshell (FMP-CNS) that exhibits a loading capacity for the hydrophobic drug paclitaxel (PTX) as high as ∼80 wt% and releases the drug in a controllable fashion under NIR irradiation (825 nm) at an intensity of 1.5 W cm(-2). The high drug loading is primarily attributed to its mesoporous structure and to the supramolecular π-stacking between FMP-CNSs and PTX molecules...
January 17, 2017: Nanoscale
https://www.readbyqxmd.com/read/28088445/synthesis-and-exploration-of-novel-radiolabeled-bombesin-peptides-for-targeting-receptor-positive-tumor
#17
Kakali De, Indranil Banerjee, Samarendu Sinha, Shantanu Ganguly
Increasing evidence of peptide receptor overexpression in various cancer cells, warrant the development of receptor specific radiolabeled peptides for molecular imaging and therapy in nuclear medicine. Gastrin-releasing-peptide (GRP) receptor, are overexpressed in a variety of human cancer cells. The present study report the synthesis and biological evaluation of new bombesin (BBN) analogs, HYNIC-Asp-[Phe(13)]BBN(7-13)-NH-CH2-CH2-CH3:BA1, HYNIC-Pro-[Tyr(13)Met(14)]BBN(7-14)NH2:BA2 as prospective tumor imaging agent with compare to BBN(7-14)NH2:BS as standard...
January 11, 2017: Peptides
https://www.readbyqxmd.com/read/28075596/yeast-microcapsule-mediated-targeted-delivery-of-diverse-nanoparticles-for-imaging-and-therapy-via-the-oral-route
#18
Xing Zhou, Xiangjun Zhang, Songling Han, Yin Dou, Mengyu Liu, Lin Zhang, Jiawei Guo, Qing Shi, Genghao Gong, Ruibing Wang, Jiang Hu, Xiaohui Li, Jianxiang Zhang
Targeting of nanoparticles to distant diseased sites after oral delivery remains highly challenging due to the existence of many biological barriers in the gastrointestinal tract. Here we report targeted oral delivery of diverse nanoparticles in multiple disease models, via a "Trojan horse" strategy based on a bioinspired yeast capsule (YC). Diverse charged nanoprobes including quantum dots (QDs), iron oxide nanoparticles (IONPs), and assembled organic fluorescent nanoparticles can be effectively loaded into YC through electrostatic force-driven spontaneous deposition, resulting in different diagnostic YC assemblies...
January 17, 2017: Nano Letters
https://www.readbyqxmd.com/read/28071889/enzyme-and-redox-dual-triggered-intracellular-release-from-actively-targeted-polymeric-micelles
#19
Lei Zhang, Yi Wang, Xiaobin Zhang, Xiao Wei, Xiang Xiong, Shaobing Zhou
Highly effective delivery of therapeutic agents into target cells using nanocarriers and subsequently rapid intracellular release are of great importance in cancer treatment. Here, we developed an enzyme and redox dual-responsive polymeric micelle with active targeting abilities to achieve rapid intracellular drug release. To overcome both its poor solubility in water and instability in the blood circulation, camptothecin (CPT) was chemically conjugated to monomethyl poly(ethylene glycol) (mPEG) via a redox-responsive linker to form polymeric prodrugs...
February 1, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28069800/label-free-raman-spectroscopy-detects-stromal-adaptations-in-premetastatic-lungs-primed-by-breast-cancer
#20
Santosh Kumar Paidi, Asif Rizwan, Chao Zheng, Menglin Cheng, Kristine Glunde, Ishan Barman
Recent advances in animal modeling, imaging technology, and functional genomics have permitted precise molecular observations of the metastatic process. However, a comprehensive understanding of the premetastatic niche remains elusive, owing to the limited tools that can map subtle differences in molecular mediators in organ-specific microenvironments. Here, we report the ability to detect premetastatic changes in the lung microenvironment, in response to primary breast tumors, using a combination of metastatic mouse models, Raman spectroscopy, and multivariate analysis of consistent patterns in molecular expression...
January 15, 2017: Cancer Research
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