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Fluorescence imaging in tumor mice

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https://www.readbyqxmd.com/read/27913947/preclinical-evaluation-of-cathepsin-based-fluorescent-imaging-system-for-cytoreductive-surgery
#1
Carlos H F Chan, Lukas F Liesenfeld, Isabel Ferreiro-Neira, James C Cusack
BACKGROUND: Cytoreductive surgery and hyperthermic intraperitoneal chemotherapy (CRS-HIPEC) is a treatment option for peritoneal surface malignancies. The ability to detect microscopic foci of peritoneal metastasis intraoperatively may ensure the completeness of cytoreduction. In this study, we evaluated the suitability of a hand-held cathepsin-based fluorescent imaging system for intraoperative detection of appendiceal and colorectal peritoneal metastasis. METHODS: Peritoneal tumors and normal peritoneal tissues were collected from patients with appendiceal and colorectal peritoneal metastasis...
December 2, 2016: Annals of Surgical Oncology
https://www.readbyqxmd.com/read/27900057/in-vitro-and-in-vivo-inhibition-of-tumor-cell-viability-by-combined-dihydroartemisinin-and-doxorubicin-treatment-and-the-underlying-mechanism
#2
Xiang Tai, Xiao-Bei Cai, Zhang Zhang, Rui Wei
The natural extract artemisinin and its derivatives have good anticancer activity. The present study aimed to investigate the in vitro inhibitory effects of combined dihydroartemisinin (DHA) and doxorubicin (DOX) treatment on a variety of tumor cell lines (HeLa, OVCAR-3, MCF-7, PC-3 and A549), as well as the underlying mechanisms. In addition, the in vivo effects of DHA and DOX were evaluated using a mouse HeLa tumor model. The HeLa, OVCAR-3, MCF-7, PC-3 and A549 cells were treated with a combination of DHA and DOX, and the effect on cell viability was detected by Cell Counting kit-8...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27890622/multi-functional-nanoparticles-as-theranostic-agents-for-treatment-imaging-in-pancreatic-cancer
#3
L R Jaidev, David Raj Chellappan, Dhiraj Vasanth Bhavsar, Ravi Ranganathan, Banudevi Sivanantham, Anuradha Subramanian, Uma Sharma, Narnamangalam R Jagannathan, Uma Maheswari Krishnan, Swaminathan Sethuraman
: Theranostics has received considerable attention since both therapy and imaging modalities can be integrated into a single nanocarrier. In this study, fluorescent iron oxide (FIO) nanoparticles and gemcitabine (G) encapsulated poly(lactide-co-glycolide) (PLGA) nanospheres (PGFIO) conjugated with human epidermal growth factor receptor 2, (HER-2) antibody (HER-PGFIO) were prepared and characterized. HER-PGFIO showed the magnetic moment of 10 emu/g, relaxivity (r2) of 773 mM(-1)s(-1) and specific absorption rate (SAR) of 183 W/g...
November 23, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27877237/near-infrared-fluorescent-nanoparticles-derived-from-hyaluronic-acid-improve-tumor-contrast-for-image-guided-surgery
#4
Tanner K Hill, Sneha S Kelkar, Nicholas E Wojtynek, Joshua J Souchek, William M Payne, Kristina Stumpf, Frank C Marini, Aaron M Mohs
Tumor tissue that remains undetected at the primary surgical site can cause tumor recurrence, repeat surgery, and treatment strategy alterations that impose a significant patient and healthcare burden. Intraoperative near infrared fluorescence (NIRF) imaging is one potential method to identify remaining tumor by visualization of NIR fluorophores that are preferentially localized to the tumor. This requires development of fluorophores that consistently identify tumor tissue in different patients and tumor types...
2016: Theranostics
https://www.readbyqxmd.com/read/27863373/tumor-targeting-adenovirus-obp-401-inhibits-primary-and-metastatic-tumor-growth-of-triple-negative-breast-cancer-in-orthotopic-nude-mouse-models
#5
Shuya Yano, Kiyoto Takehara, Hiroyuki Kishimoto, Hiroshi Tazawa, Yasuo Urata, Shunsuke Kagawa, Michael Bouvet, Toshiyoshi Fujiwara, Robert M Hoffman
Our laboratory previously developed a highly-invasive, triple-negative breast cancer (TNBC) variant using serial orthotopic implantation of the human MDA-MB-231 cell line in nude mice. The isolated variant was highly-invasive in the mammary gland and lymphatic channels and metastasized to lymph nodes in 10 of 12 mice compared to 2 of 12 of the parental cell line. In the present study, the tumor-selective telomerase dependent OBP-401 adenovirus was injected intratumorally (i.t.) (1 × 108 PFU) when the high-metastatic MDA-MB-231 primary tumor expressing red fluorescent protein (MDA-MB-231-RFP) reached approximately 500 mm3 (diameter; 10 mm)...
November 11, 2016: Oncotarget
https://www.readbyqxmd.com/read/27854107/assessing-the-efficacy-of-coherent-anti-stokes-raman-scattering-microscopy-for-the-detection-of-infiltrating-glioblastoma-in-fresh-brain-samples
#6
Roberta Galli, Ortrud Uckermann, Achim Temme, Elke Leipnitz, Matthias Meinhardt, Edmund Koch, Gabriele Schackert, Gerald Steiner, Matthias Kirsch
Coherent anti-Stokes Raman scattering (CARS) microscopy is an emerging technique for identification of brain tumors. However, tumor identification by CARS microscopy on bulk samples and in vivo has been so far verified retrospectively on histological sections, which only provide a gross reference for the interpretation of CARS images without matching at cellular level. Therefore, fluorescent labels were exploited for direct assessment of the interpretation of CARS images of solid and infiltrative tumors. Glioblastoma cells expressing green fluorescent protein (GFP) were used for induction of tumors in mice (n = 7)...
March 11, 2016: Journal of Biophotonics
https://www.readbyqxmd.com/read/27853366/development-of-plga-lipid-nanoparticles-with-covalently-conjugated-indocyanine-green-as-a-versatile-nanoplatform-for-tumor-targeted-imaging-and-drug-delivery
#7
Yu Xin, Tie Liu, Chenlong Yang
We have prepared novel poly(d,l-lactide-co-glycolide) (PLGA) lipid nanoparticles (PNPs) that covalently conjugate folic acid (FA) and indocyanine green (ICG), in addition to encapsulating resveratrol (RSV) (FA-RSV/ICG-PLGA-lipid NPs, abbreviated as FA-RIPNPs); these nanoparticles have been developed for simultaneous targeted delivery of anticancer drug and fluorescence imaging. The FA-RIPNPs, with an average particle size of 92.8±2.1 nm, were prepared by a facile self-assembly-and-nanoprecipitation method, and they showed excellent stability and biocompatibility characteristics...
2016: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/27848167/increased-active-tumor-targeting-by-an-%C3%AE-v%C3%AE-3-targeting-and-cell-penetrating-bifunctional-peptide-mediated-dendrimer-based-conjugate
#8
Pengkai Ma, Huajun Yu, Xuemei Zhang, Hongjie Mu, Yongchao Chu, Ling Ni, Pingping Xing, Yiyun Wang, Kaoxiang Sun
PURPOSE: A bifunctional RGDTAT peptide-modified PEG-PAMAM dendrimer conjugate RGDTAT-PEG-PAMAM (RTPP) was established for the targeted treatment of αvβ3-overexpressing tumor cells. METHODS: The RGDTAT peptide was synthesized and attached to PAMAM using PEG to construct the RTPP conjugate. The methotrexate (MTX) encapsulated RTPPM complex was prepared and characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM) and in vitro release...
November 15, 2016: Pharmaceutical Research
https://www.readbyqxmd.com/read/27825878/an-artemisinin-mediated-ros-evolving-and-dual-protease-light-up-nanocapsule-for-real-time-imaging-of-lysosomal-tumor-cell-death
#9
Liwei Huang, Yingping Luo, Xian Sun, Huangxian Ju, Jiangwei Tian, Bo-Yang Yu
Lysosomes are critical organelles for cellular homeostasis and can be used as potential targets to kill tumor cells from inside. Many photo-therapeutic methods have been developed to overproduce reactive oxygen species (ROS) to trigger lysosomal membrane permeabilization (LMP)-associated cell death pathway. However, these technologies rely on extra irradiation to activate the photosensitizers, which limits the applications in treating deep seated tumors and widespread metastatic lesions. This work reports a multifunctional nanocapsule to achieve targeted lysosomal tumor cell death without irradiation and real-time monitoring of drug effect through encapsulating artemisinin and dual protease light-up nanoprobe in a folate-functionalized liposome...
October 11, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27809256/in-vivo-follow-up-of-brain-tumor-growth-via-bioluminescence-imaging-and-fluorescence-tomography
#10
Coralie Genevois, Hugues Loiseau, Franck Couillaud
Reporter gene-based strategies are widely used in experimental oncology. Bioluminescence imaging (BLI) using the firefly luciferase (Fluc) as a reporter gene and d-luciferin as a substrate is currently the most widely employed technique. The present paper compares the performances of BLI imaging with fluorescence imaging using the near infrared fluorescent protein (iRFP) to monitor brain tumor growth in mice. Fluorescence imaging includes fluorescence reflectance imaging (FRI), fluorescence diffuse (optical) tomography (fDOT), and fluorescence molecular Imaging (FMT(®))...
October 31, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27808492/dual-targeting-magnetic-plga-nanoparticles-for-codelivery-of-paclitaxel-and-curcumin-for-brain-tumor-therapy
#11
Yanna Cui, Meng Zhang, Feng Zeng, Hongyue Jin, Qin Xu, Yongzhuo Huang
Chemotherapy is one of the most important strategies for glioma treatment. However, the "impermeability" of the blood-brain barrier (BBB) impedes most chemotherapeutics to enter the brain, thereby rendering very few drugs suitable for glioma therapy, letting along application of combination of chemotherapeutics. Thereby, it is a pressing need to overcome the obstacles. A dual-targeting strategy was developed by combination of magnetic-guidance and the transferrin receptor-binding peptide T7-mediated active targeting delivery...
November 3, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27807977/nature-inspired-smart-dna-nanodoctor-for-activatable-in-vivo-cancer-imaging-and-in-situ-drug-release-based-on-recognition-triggered-assembly-of-split-aptamer
#12
Yanli Lei, Jinlu Tang, Hui Shi, Xiaosheng Ye, Xiaoxiao He, Fengzhou Xu, Lv'an Yan, Zhenzhen Qiao, Kemin Wang
DNA-based activatable theranostic nanoprobes are still unmet for in vivo applications. Here, by utilizing the "induced-fit effect", a smart split aptamer-based activatable theranostic probe (SATP) was first designed as "nanodoctor" for cancer-activated in vivo imaging and in situ drug release. The SATP assembled with quenched fluorescence and stable drug loading in its free state. Once binding to target proteins on cell surface, the SATP disassembled due to recognition-triggered reassembly of split aptamers with activated signals and freed drugs...
November 3, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27795740/pillar-5-arene-based-amphiphilic-supramolecular-brush-copolymer-fabrication-controllable-self-assembly-and-application-in-self-imaging-targeted-drug-delivery
#13
Guocan Yu, Run Zhao, Dan Wu, Fuwu Zhang, Li Shao, Jiong Zhou, Jie Yang, Guping Tang, Xiaoyuan Chen, Feihe Huang
Supramolecular brush copolymers have attracted continuing interest due to their unusual architectures, fascinating properties, and potential applications in many fields involving smart stimuli-responsive drug delivery systems. Herein, the first pillararene-based amphiphilic supramolecular brush copolymer (P5-PEG-Biotin⊃PTPE) was constructed on the basis of the host-guest molecular recognition between a water-soluble pillar[5]arene (P5) and a viologen salt (M). P5-PEG-Biotin⊃PTPE self-assembled into supramolecular nanoparticles (SNPs), which were utilized as a self-imaging drug delivery vehicle by taking advantage of the aggregation-induced emission (AIE) effect...
October 28, 2016: Polymer Chemistry
https://www.readbyqxmd.com/read/27793401/near-infrared-intraoperative-molecular-imaging-can-locate-metastases-to-the-lung
#14
Jane Keating, Andrew Newton, Ollin Venegas, Sarah Nims, Ryan Zeh, Jarrod Predina, Charuhas Deshpande, John Kucharczuk, Shuming Nie, E James Delikatny, Sunil Singhal
BACKGROUND: Pulmonary metastasectomy is widely accepted for many tumor types because it may prolong survival and potentially cure some patients. However, intraoperative localization of pulmonary metastases can be technically challenging. We propose that intraoperative near-infrared (NIR) molecular imaging can be used as an adjunct during disease localization. METHODS: We inoculated 50 C57BL/6 mice with Lewis lung carcinoma (LLC) flank tumors. After flank tumor growth, mice were injected through the tail vein with indocyanine green (ICG) before operation, and intraoperative imaging was used to detect pulmonary metastases...
October 25, 2016: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/27785019/ge11-peptide-modified-and-reduction-responsive-hyaluronic-acid-based-nanoparticles-induced-higher-efficacy-of-doxorubicin-for-breast-carcinoma-therapy
#15
Danrong Hu, Omar Mezghrani, Lei Zhang, Yi Chen, Xue Ke, Tianyuan Ci
Novel breast carcinoma dual-targeted redox-responsive nanoparticles (NPs) based on cholesteryl-hyaluronic acid conjugates were designed for intracellular delivery of the antitumor drug doxorubicin (DOX). A series of reduction-responsive hyaluronic acid derivatives grafted with hydrophobic cholesteryl moiety (HA-ss-Chol) and GE11 peptide conjugated HA-ss-Chol (GE11-HA-ss-Chol) were synthesized. The obtained conjugates showed attractive self-assembly characteristics and high drug loading capacity. GE11-HA-ss-Chol NPs were highly stable under conditions mimicking normal physiological conditions, while showing a fast degradation of the vehicle's structure and accelerating the drug release dramatically in the presence of intracellular reductive environment...
2016: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/27779702/extract-of-caulis-spatholobi-a-novel-blocker-targeting-tumor-cell%C3%A2-induced-platelet-aggregation-inhibits-breast-cancer-metastasis
#16
Xi Chen, Qi Li, Xiao-Xi Kan, Ya-Jie Wang, Yu-Jie Li, Qing Yang, Hong-Bin Xiao, Ying Chen, Xiao-Gang Weng, Wei-Yan Cai, Xiao-Xin Zhu
Metastasis of breast cancer is the vital step for malignant progression. During such a process, hematogenous metastasis is an indispensable approach for the dissemination of cancer cells. A platelet, contributes to hypercoagulable state, and is also identified the crucial factor in the coagulation system for supporting metastasis. Therefore, the relationship of a platelet and a tumor cell plays a critical role in tumor cell metastasis. Consequently, inhibiting tumor cell‑induced platelet aggregation (TCIPA) is recongnized as a crucial target on suppression of tumor metastasis such as aspirin (ASA)...
October 19, 2016: Oncology Reports
https://www.readbyqxmd.com/read/27762044/cathepsin%C3%A2-b-specific-metabolic-precursor-for-in%C3%A2-vivo-tumor-specific-fluorescence-imaging
#17
Man Kyu Shim, Hong Yeol Yoon, Ju Hee Ryu, Heebeom Koo, Sangmin Lee, Jae Hyung Park, Jong-Ho Kim, Seulki Lee, Martin G Pomper, Ick Chan Kwon, Kwangmeyung Kim
Recently, metabolic glycoengineering with bioorthogonal click reactions has focused on improving the tumor targeting efficiency of nanoparticles as delivery vehicles for anticancer drugs or imaging agents. It is the key technique for developing tumor-specific metabolic precursors that can generate unnatural glycans on the tumor-cell surface. A cathepsin B-specific cleavable substrate (KGRR) conjugated with triacetylated N-azidoacetyl-d-mannosamine (RR-S-Ac3 ManNAz) was developed to enable tumor cells to generate unnatural glycans that contain azide groups...
November 14, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27760789/the-biodistribution-and-immune-suppressive-effects-of-breast-cancer-derived-exosomes
#18
Shu Wen Wen, Jaclyn Sceneay, Luize Goncalves Lima, Christina S F Wong, Melanie Becker, Sophie Krumeich, Richard J Lobb, Vanessa Castillo, Ke Ni Wong, Sarah Ellis, Belinda S Parker, Andreas Möller
Small membranous secretions from tumor cells, termed exosomes, contribute significantly to intercellular communication and subsequent reprogramming of the tumor microenvironment. Here, we use optical imaging to determine that exogenously administered fluorescently labeled exosomes derived from highly metastatic murine breast cancer cells distributed predominantly to the lung of syngeneic mice, a frequent site of breast cancer metastasis. At the sites of accumulation, exosomes were taken up by CD45(+) bone marrow-derived cells...
December 1, 2016: Cancer Research
https://www.readbyqxmd.com/read/27749069/highly-effective-dual-function-near-infrared-nir-photosensitizer-for-fluorescence-imaging-and-photodynamic-therapy-pdt-of-cancer
#19
Nayan Patel, Paula Pera, Penny Joshi, Mykhaylo Dukh, Walter A Tabaczynski, Kevin E Siters, Mark Kryman, Ravindra R Cheruku, Farukh Durrani, Joseph R Missert, Ramona Watson, Tymish Y Ohulchanskyy, Erin Tracy, Heinz Baumann, Ravindra K Pandey
We report herein, the synthesis and biological efficacy of near-infrared (NIR), bacteriochlorin analogs: 3-(1'-butyloxy)ethyl-3-deacetyl-bacteriopurpurin-18-N-butylimide methyl ester (3) and the corresponding carboxylic acid 10. In in vitro assays, compared to its methyl ester analog 3, the corresponding carboxylic acid derivative 10 showed higher photosensitizing efficacy. However, due to drastically different pharmacokinetics in vivo, the PS 3 (HPLC purity > 99%) showed higher tumor uptake and long-term tumor cure than 10 (HPLC purity > 96...
October 17, 2016: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/27748558/multispectral-autofluorescence-imaging-for-detection-of-cervical-lesions-a-preclinical-study
#20
Soo-Jin Bae, Dae-Sic Lee, Vladimir Berezin, Uk Kang, Keun-Ho Lee
AIM: The aim of this study was to develop a novel optical imaging system for detecting protoporphyrin IX (PpIX) autofluorescence, to prove that PpIX autofluorescence is as useful as 5-aminolevulinic acid (5-ALA)-induced fluorescence for detecting and localizing cervical cancer, and to monitor the change in PpIX autofluorescence or induced PpIX fluorescence before, during, and after photodynamic therapy (PDT). METHODS: TC-1 cells - highly tumorigenic cells immortalized using human papillomavirus type 16 proteins E6 and E7 - were subcutaneously grafted into the thighs of nude mice...
October 17, 2016: Journal of Obstetrics and Gynaecology Research
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