keyword
MENU ▼
Read by QxMD icon Read
search

bax

keyword
https://www.readbyqxmd.com/read/28944912/dhhp%C3%A2-6-attenuates-cerebral-ischemia%C3%A2-reperfusion-injury-in-rats-through-the-inhibition-of-apoptosis
#1
Yingshi Ji, Xin Yan, Yang Hu, Huan Xue, Jianfeng Sun, Huaqiu Chen, Jiayu Zhang, Liping Wang, Baigong Xue, Li Sun
As a novel reactive oxygen species (ROS) scavenger, deuterohemin His peptide‑6 (DhHP‑6) has been demonstrated to prolong the lifespan of Caenorhabditis elegans and has also exhibited protective effects in myocardial ischemia‑reperfusion injury. Whether similar effects occur during cerebral ischemia‑reperfusion (CIR) injury remains to be elucidated. The present study evaluated the function of DhHP‑6 and its underlying mechanisms in a middle cerebral artery occlusion (MCAO) model in rats. The focal transient MCAO model was implemented using the Longa method of ischemia for 2 h followed by reperfusion for 22 h in male Wistar rats...
September 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944905/myricitrin-decreases-traumatic-injury-of-the-spinal-cord-and-exhibits-antioxidant-and-anti%C3%A2-inflammatory-activities-in-a-rat-model-via-inhibition-of-cox%C3%A2-2-tgf%C3%A2-%C3%AE-1-p53-and-elevation-of-bcl%C3%A2-2-bax-signaling-pathway
#2
Yang Lei
Myricitrin has multiple effects, including antagonism of platelet activating factor, regulation of blood sugar levels, oxidation resistance, protection of the liver and the relieving of ethylism. The present study evaluated how myricitrin weakens traumatic injury of the spinal cord (TISC), and exhibits antioxidant and anti‑inflammatory activities in a rat model. TISC model rats were injected intraperitoneally with 5, 10 or 30 mg/kg/day of myricitrin for 5 days. Basso‑Beattie‑Bresnahan evaluation of locomotion and water content of spinal cord were used to analyze the effects of myricitrin on TISC...
September 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944894/glucosylceramide-synthase-regulates-the-proliferation-and-apoptosis-of-liver-cells-in%C3%A2-vitro-by-bcl%C3%A2-2-bax-pathway
#3
Jun-Feng Li, Su-Jun Zheng, Li-Li Wang, Shuang Liu, Feng Ren, Yu Chen, Li Bai, Mei Liu, Zhong-Ping Duan
Our previous study found that glucosylceramide, a type of sphingolipids, was associated with liver inflammation and fibrosis. Glucosylceramide is generated by glucosylceramide synthase (GCS), which is encoded by the UDP‑glucose ceramide glucosyltransferase (UGCG) gene. GCS is a key enzyme to regulate the physiological activity of cells. However, the role of GCS in hepatic cells remains unclear. The aim of the present study was to explore the mechanism of GCS in the proliferation and apoptosis of liver cells...
September 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944886/sulforaphane-induces-p53%C3%A2-deficient-sw480-cell-apoptosis-via-the-ros%C3%A2-mapk-signaling-pathway
#4
Hai Lan, Hongyin Yuan, Congyao Lin
Sulforaphane (SFN) has been revealed to inhibit the growth and induce apoptosis of cancer cells. However, the detailed anticancer effects of SFN on p53‑deficient colon cancer cells has yet to be clearly elucidated. The present study employed p53‑deficient SW480 cells to establish an SFN‑induced in vitro model of apoptosis. The critical events leading to apoptosis were then evaluated in SFN‑treated p53‑deficient SW480 cells, by performing an MTT assay, flow cytometry, western blotting and ELISA. The results demonstrated that SFN at concentrations of 5, 10, 15 and 20 µM induced mitochondria‑associated cell apoptosis, which was further confirmed by disruption of the mitochondrial membrane potential, an increase in the Bax/Bcl‑2 ratio, as well as activation of caspase‑3, ‑7 and ‑9...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944872/isoflurane-exposure-regulates-the-cell-viability-and-bdnf-expression-of-astrocytes-via-upregulation-of-trek%C3%A2-1
#5
Cui-Hong Zhou, Ya-Hong Zhang, Fen Xue, Shan-Shan Xue, Yun-Chun Chen, Ting Gu, Zheng-Wu Peng, Hua-Ning Wang
Neonatal isoflurane exposure in rodents disrupts hippocampal cognitive functions, including learning and memory, and astrocytes may have an important role in this process. However, the molecular mechanisms underlying this disruption are not fully understood. The present study investigated the role of TWIK‑related K+ channel (TREK‑1) in isoflurane‑induced cognitive impairment. Lentiviruses were used to overexpress or knockdown TREK‑1 in astrocytes exposed to increasing concentrations of isoflurane or O2 for 2 h...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944861/panax-notoginseng-saponins-attenuates-sevoflurane%C3%A2-induced-nerve-cell-injury-by-modulating-akt-signaling-pathway
#6
Xu Yang, Sun Yang, Chen Hong, Wang Yu, Wang Guonian
General anesthesia in patients with or at risk for neuronal injury remains challenging due to the neurotoxic effects of volatile anesthetics. One inhalation anesthetic, sevoflurane, induces neuronal damage, including neuroapoptosis, and learning and memory impairment. Panax Notoginseng Saponins (PNS) is the active ingredient of Sanqui and has been reported to exert neuroprotective effects. In the current study, the protective effect of PNS on sevoflurane‑induced nerve cell injury was explored. Cell proliferation was significantly reduced in a dose‑dependent manner following stimulation with sevoflurane...
September 19, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944839/alteration-of-oxidative-stress-and-inflammatory-cytokines-induces-apoptosis-in-diabetic-nephropathy
#7
Jibin Sha, Bo Sui, Xiaoqing Su, Qingfang Meng, Chenggang Zhang
Diabetic nephropathy (DN) is one of the most significant long‑term complications in terms of morbidity and mortality for diabetic patients; however, the exact cause remains unknown. To address this, the DN model was established, and oxidative stress indexes, including malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH‑Px), and inflammatory cytokines, includinginterleukin‑6 (IL‑6), tumor necrosis factor‑alpha (TNF‑α) and transforming growth factor‑beta (TGF‑β), were examined by ELISA...
September 19, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944567/differentiation-of-mesenchymal-stem-cells-to-germ-like-cells-under-induction-of-sertoli-cell-conditioned-medium-and-retinoic-acid
#8
R Ghaem Maghami, T Mirzapour, A Bayrami
The aim of this research was to find a way to differentiate germ cells from umbilical cord Wharton's jelly mesenchymal stem cells (MSCs) to support in vitro spermatogenesis. A small piece of Wharton's jelly was cultured in high-glucose Dulbecco's modified Eagle's medium in present of 10% foetal calf serum. After the fourth passage, the cells were isolated and cultured in Sertoli cell-conditioned medium under induction of two different doses of retinoic acid (10(-5) , 10(-6)  m). The differentiation of MSC to germ-like cells was evaluated by expression of Oct4, Nanog, Plzf, Stra8 and Prm1 genes during different days of culture through qPCR...
September 25, 2017: Andrologia
https://www.readbyqxmd.com/read/28943902/khat-promotes-human-breast-cancer-mda-mb-231-cell-apoptosis-via-mitochondria-and-mapk-associated-pathways
#9
Yu Lu, Yanyan Li, Min Xiang, Jie Zhou, Juan Chen
Khat (Catha edulis Forsk) is a flowering evergreen plant in Eastern Africa and Southwestern Arabia. Consumption of Khat has been associated with the development of oral cancer, but its mechanism of action on the molecular level remains unclear. The present study demonstrated the cytotoxic effect of khat extracts on the human breast cancer cell line MDA-MB-231. Trypan blue exclusion assays, flow cytometry, fluorescent and electron microscopy, as well as western blotting were used to analyze the effects of Khat on the cell viability of breast cancer cells, expression of apoptotic-associated proteins and the levels of reactive oxygen species (ROS)...
October 2017: Oncology Letters
https://www.readbyqxmd.com/read/28943754/pinus-densiflora-bark-extract-prevents-selenite-induced-cataract-formation-in-the-lens-of-sprague-dawley-rat-pups
#10
Jun Kim, Se-Young Choung
PURPOSE: Rat pups treated with sodium selenite are typically used as an in vivo model to mimic age-related nuclear cataract. Reactive oxygen species (ROS) production, lipid peroxidation, reduction of antioxidant enzymes, crystalline proteolysis, and apoptosis are considered factors that contribute to pathogenesis of age-related nuclear cataract. In the present study, we investigated whether Pinus densiflora bark extract has potential to prevent cataract formation and elucidated the underlying mechanism...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28943529/quisqualis-indica-improves-benign-prostatic-hyperplasia-by-regulating-prostate-cell-proliferation-and-apoptosis
#11
Charith Ub Wijerathne, Hee-Seon Park, Hye-Yun Jeong, Ji-Won Song, Og-Sung Moon, Young-Won Seo, Young-Suk Won, Hwa-Young Son, Jong-Hwan Lim, Sung-Hum Yeon, Hyo-Jung Kwun
Quisqualis indica (QI) has been used for treating disorders such as stomach pain, constipation, and digestion problem. This study was aimed to evaluate the therapeutic efficacy of QI extract on treating benign prostatic hyperplasia (BPH) in LNCaP human prostate cancer cell line and a testosterone-induced BPH rat model. LNCaP cells were treated with QI plus testosterone propionate (TP), and androgen receptor (AR) and prostate specific antigen (PSA) expression levels were assessed by Western blotting. To induce BPH, the rats were subjected to a daily subcutaneous injection of TP (3 mg/kg) for 4 wk...
September 22, 2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28943243/hucmsc-exosome-transported-14-3-3%C3%AE-prevents-the-injury-of-cisplatin-to-hk-2-cells-by-inducing-autophagy-in-vitro
#12
REVIEW
Juanjuan Wang, Haoyuan Jia, Bin Zhang, Lei Yin, Fei Mao, Jing Yu, Cheng Ji, Xiao Xu, Yongmin Yan, Wenrong Xu, Hui Qian
BACKGROUND AIMS: On the basis of previous studies, exosomes secreted by human umbilical cord mesenchymal stromal cell (hucMSC-ex) could prevent and repair acute kidney injury induced by cisplatin in rats. However, its potential mechanism is still unclear. In the present study, the model with hucMSC-ex pretreated human renal tubular epithelial cell lines HK-2 that could prevent the injury of cisplatin was successfully established. METHODS: First, we pretreated the HK-2 cells with hucMSC-ex for 24 h...
September 21, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28942508/role-of-inducible-nitric-oxide-synthase-in-myocardial-ischemia-reperfusion-injury-in-sleep-deprived-rats
#13
Sajad Jeddi, Asghar Ghasemi, Alireza Asgari, Amir Nezami-Asl
INTRODUCTION: REM sleep deprivation (SD) decreases tolerance of the rat heart to ischemia-reperfusion (IR) injury; the underlying mechanisms, however, are unknown. This study aimed at determining whether changes in iNOS, Bax, and Bcl-2 gene expression are involved in this detrimental effect. METHOD: SD was induced by flowerpot technique for a period of 4 days. This method is simple and able to induce sleep fragmentation which occurs as one of the sleep disorder symptoms in clinical conditions...
September 23, 2017: Sleep & Breathing, Schlaf & Atmung
https://www.readbyqxmd.com/read/28942283/edaravone-ameliorates-compression-induced-damage-in-rat-nucleus-pulposus-cells
#14
Hui Lin, Xuan Ma, Bai-Chuan Wang, Lei Zhao, Jian-Xiang Liu, Fei-Fei Pu, Yi-Qiang Hu, Hong-Zhi Hu, Zeng-Wu Shao
AIMS: Edaravone is a strong free radical scavenger most used for treating acute ischemic stroke. In this study we investigated the protective effects and underlying mechanisms of edaravone on compression-induced damage in rat nucleus pulposus (NP) cells. MATERIALS AND METHODS: Cell viability was determined using MTT assay methods. NP cell apoptosis was measured by Hoechst 33,258 staining and Annexin V/PI double staining. Intracellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP), and intracellular calcium ([Ca(2+)]i) were determined by fluorescent probes DCFH-DA, JC-1 and Fluo-3/AM, respectively...
September 20, 2017: Life Sciences
https://www.readbyqxmd.com/read/28942194/the-2-oxoglutarate-carrier-promotes-liver-cancer-by-sustaining-mitochondrial-gsh-despite-cholesterol-loading
#15
Anna Baulies, Joan Montero, Nuria Matías, Naroa Insausti, Oihana Terrones, Gorka Basañez, Carmen Vallejo, Laura Conde de La Rosa, Laura Martinez, David Robles, Albert Morales, Joaquin Abian, Montserrat Carrascal, Keigo Machida, Dinesh B U Kumar, Hidekazu Tsukamoto, Neil Kaplowitz, Carmen Garcia-Ruiz, José C Fernández-Checa
Cancer cells exhibit mitochondrial cholesterol (mt-cholesterol) accumulation, which contributes to cell death resistance by antagonizing mitochondrial outer membrane (MOM) permeabilization. Hepatocellular mt-cholesterol loading, however, promotes steatohepatitis, an advanced stage of chronic liver disease that precedes hepatocellular carcinoma (HCC), by depleting mitochondrial GSH (mGSH) due to a cholesterol-mediated impairment in mGSH transport. Whether and how HCC cells overcome the restriction of mGSH transport imposed by mt-cholesterol loading to support mGSH uptake remains unknown...
September 14, 2017: Redox Biology
https://www.readbyqxmd.com/read/28942112/synthesis-structure-activity-relationships-and-preliminary-mechanism-of-action-of-novel-water-soluble-4-quinolone-3-carboxamides-as-antiproliferative-agents
#16
Zeyan Zhang, Xingpeng Xiao, Tong Su, Jinyi Wu, Jianwei Ren, Jiongchang Zhu, Xiaodong Zhang, Rihui Cao, Runlei Du
A series of novel water-soluble 4-quinolone-3-carboxamides was prepared and evaluated as antiproliferative agents. Preliminary results indicated that most compounds tested in this study showed potent antiproliferative potencies against human tumor cell lines, and compound 8k was found to be the most potent antiproliferative agents with IC50 value of lower than 10 μM against nine human tumor cell lines. These results suggested that (1) the alkylamino side chain substituent was the advisable pharmacophoric group for the enhanced antiproliferative activities; (2) the length of the alkylamino side chain moiety also affected their antiproliferative potencies, and three methylene units were more favorable; (3) introducing arylated alkyl substituent into N1-position of quinolone facilitated antiproliferative activities of this class of compounds...
September 14, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28940036/toxicarioside-n-induces-apoptosis-in-human-gastric-cancer-sgc-7901-cell-by-activating-the-p38mapk-pathway
#17
Huan-Ge Zhao, Song-Lin Zhou, Ying-Ying Lin, Hao-Fu Dai, Feng-Ying Huang
Natural plant compounds with potent proliferation inhibition and apoptosis induction properties have been screened as novel anticancer drugs. Toxicarioside N (Tox N) was isolated from the seeds of the tropical plant Antiaris toxicaria in Hainan province, China. To our knowledge, the effects that Tox N has on the apoptosis of SGC-7901 cells and its potential mechanism have never been investigated. In this study, we detected the anticancer activities of Tox N and explored the potential mechanism in the human gastrointestinal cancer cell line SGC-7901...
September 22, 2017: Archives of Pharmacal Research
https://www.readbyqxmd.com/read/28939896/mir-155-mediates-arsenic-trioxide-resistance-by-activating-nrf2-and-suppressing-apoptosis-in-lung-cancer-cells
#18
Shiyan Gu, Yanhao Lai, Hongyu Chen, Yuan Liu, Zunzhen Zhang
Arsenic trioxide (ATO) resistance is a challenging problem in chemotherapy. However, the underlying mechanisms remain to be elucidated. In this study, we identified a high level of expression of miR-155 in a human lung adenocarcinoma A549R cell line that is highly resistant to ATO. We showed that the high level of miR-155 was associated with increased levels of cell survival, colony formation, cell migration and decreased cellular apoptosis, and this was mediated by high levels of Nrf2, NAD(P)H quinone oxidoreductase 1 (NQO1), heme oxygenase-1 (HO-1) and a high ratio of Bcl-2/Bax...
September 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28939271/perfluorooctane-sulphonate-induces-oxidative-hepatic-damage-via-mitochondria-dependent-and-nf-%C3%AE%C2%BAb-tnf-%C3%AE-mediated-pathway
#19
Rui Han, Mingxian Hu, Qiang Zhong, Chong Wan, Limin Liu, Fang Li, Fang Zhang, Wenjun Ding
Perfluorooctane sulphonate (PFOS) has been reported to accumulate in liver and cause damage. The molecular mechanism of the PFOS-induced hepatotoxicity has not been completely elucidated. The aim of the present study was to investigate whether PFOS-induced oxidative stress plays an important role in liver damage, and if so, what pathway it undergoes for the mechanism of its toxicological action. Male Sprague-Dawley (SD) rats were orally administrated with PFOS at single dose of 1 or 10 mg/kg body weight for 28 consecutive days...
August 15, 2017: Chemosphere
https://www.readbyqxmd.com/read/28938844/mcur1-mediated-mitochondrial-calcium-signaling-facilitates-cell-survival-of-hepatocellular-carcinoma-via-ros-dependent-p53-degradation
#20
Jinliang Xing, Tingting Ren, Jiaojiao Wang, Hui Zhang, Peng Yuan, Jianjun Zhu, Yousheng Wu, Qichao Huang, Xu Guo, Jing Zhang, Lele Ji, Jibin Li, Hongxin Zhang, Hushan Yang
AIMS: Levels of the Mitochondrial Calcium uniporter regulator 1 (MCUR1) increases during development of hepatocellular carcinoma (HCC). However, mechanistic understanding of how mitochondrial Ca2+ homeostasis is remodeled and its functional roles remains limited in cancers, especially in HCC. RESULTS: MCUR1 was frequently upregulated in HCC cells to enhance the Ca2+ uptake into mitochondria in a MCU-dependent manner, which significantly facilitated cell survival by promoting cell proliferation and inhibiting mitochondria-dependent intrinsic apoptosis, and thus contributed to poor prognosis of HCC patients...
September 22, 2017: Antioxidants & Redox Signaling
keyword
keyword
50349
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"