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https://www.readbyqxmd.com/read/28538980/downregulation-of-lysyl-oxidase-protects-retinal-endothelial-cells-from-high-glucose-induced-apoptosis
#1
Dongjoon Kim, Robert P Mecham, Philip C Trackman, Sayon Roy
Purpose: To investigate the effect of reducing high glucose (HG)-induced lysyl oxidase (LOX) overexpression and increased activity on retinal endothelial cell apoptosis. Methods: Rat retinal endothelial cells (RRECs) were grown in normal (N) or HG (30 mM glucose) medium for 7 days. In parallel, RRECs were grown in HG medium and transfected with LOX small interfering RNA (siRNA), scrambled siRNA as control, or exposed to β-aminopropionitrile (BAPN), a LOX inhibitor...
May 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28538152/apoptotic-bax-at-oxidatively-stressed-mitochondrial-membranes-lipid-dynamics-and-permeabilization
#2
Artur Peter Günther Dingeldein, Šárka Pokorná, Martin Lidman, Tobias Sparrman, Radek Šachl, Martin Hof, Gerhard Gröbner
Mitochondria are crucial compartments of eukaryotic cells because they function as the cellular power plant and play a central role in the early stages of programmed cell death (apoptosis). To avoid undesired cell death, this apoptotic pathway is tightly regulated by members of the Bcl-2 protein family, which interact on the external surface of the mitochondria, i.e., the mitochondrial outer membrane (MOM), and modulate its permeability to apoptotic factors, controlling their release into the cytosol. A growing body of evidence suggests that the MOM lipids play active roles in this permeabilization process...
May 23, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28537893/alpha-mangostin-induces-apoptosis-through-activation-of-reactive-oxygen-species-and-ask1-p38-signaling-pathway-in-cervical-cancer-cells
#3
Chien-Hsing Lee, Tsung-Ho Ying, Hui-Ling Chiou, Shu-Ching Hsieh, Shiua-Hua Wen, Ruey-Hwang Chou, Yi-Hsien Hsieh
Alpha-mangostin, a natural xanthonoid, has been reported to possess the anti-cancer property in various types of human cancer. However, its effects and mechanism of α-mangostin in cervical cancer remain unclear. We found that α-mangostin effectively inhibited cell viability, resulted in loss of mitochondrial membrane potential (MMP), release of cytochrome C, increase of Bax, decrease of Bcl-2, and activation of caspase-9/caspase-3 cascade in cervical cancer cells. Alpha-mangostin elevated the contents of reactive oxygen species (ROS) to activate p38...
May 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28535015/the-circadian-clock-gene-per2-plays-an-important-role-in-tumor-suppression-through-regulating-tumor-associated-genes-in-human-oral-squamous-cell-carcinoma
#4
Xiaoli Su, Dan Chen, Kai Yang, Qin Zhao, Dan Zhao, Xiaoqiang Lv, Yiran Ao
Low expression of the clock gene PER2 is closely related to carcinogenesis and the development of cancer; however, the mechanism of the low expression of PER2 that led to cell malignant transformation remains unclear. This study used RNA interference (RNAi) technology to silence PER2 in SCC15 human oral squamous cell carcinoma (OSCC) cells. Then it was found that the ability of cancer cell proliferation, migration, and invasion were markedly increased (P<0.05), and the ability of cancer cell apoptosis and the number of cells in G1/G0 phase were markedly reduced (P<0...
May 19, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28534932/inhibition-of-prostate-cancer-rm1-cell-growth-in%C3%A2-vitro-by-hydroxyapatite-nanoparticle%C3%A2-delivered-short-hairpin-rnas-against-stat3
#5
Zuowen Liang, Hongliang Wang, Baofeng Guo, Fubiao Li, Jinsha Liu, Zhewen Liu, Libo Xu, Wenjing Yun, Xuejian Zhao, Ling Zhang
The present study investigated the effect of signal transducer and activator of transcription 3 (Stat3) interference on RM1 prostate cancer cell viability in vitro, using plasmid‑based Stat3 specific short hairpin RNA (sh‑Stat3) delivered by hydroxyapatite nanoparticles (HAP). HAP carrying sh‑Stat3 plasmids were transfected into tumor cells. MTT assays were used to measure RM1 cell viability 24 and 48 h following transfection, and the apoptosis rate and cell cycle phase distribution were determined by flow cytometry...
May 16, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28534825/a-novel-combination-of-withaferin-a-and-sulforaphane-inhibits-epigenetic-machinery-cellular-viability-and-induces-apoptosis-of-breast-cancer-cells
#6
Kendra J Royston, Neha Udayakumar, Kayla Lewis, Trygve O Tollefsbol
With cancer often classified as a disease that has an important epigenetic component, natural compounds that have the ability to regulate the epigenome become ideal candidates for study. Humans have a complex diet, which illustrates the need to elucidate the mechanisms of interaction between these bioactive compounds in combination. The natural compounds withaferin A (WA), from the Indian winter cherry, and sulforaphane (SFN), from cruciferous vegetables, have numerous anti-cancer effects and some report their ability to regulate epigenetic processes...
May 19, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28532227/peptides-extracted-from-edible-mushroom-lentinus-squarrosulus-induces-apoptosis-in-human-lung-cancer-cells
#7
Arisara Prateep, Somruethai Sumkhemthong, Maneewan Suksomtip, Pithi Chanvorachote, Chatchai Chaotham
CONTEXT: Lentinus squarrosulus Mont. (Polyporaceae) is an interesting source of diverse bioactive compounds. OBJECTIVE: This is the first study of the anticancer activity and underlying mechanism of peptides extracted from Lentinus squarrosuls. MATERIALS AND METHODS: Peptides were isolated from the aqueous extract of L. squarrosulus by employing solid ammonium sulphate precipitation. They were further purified by ion-exchange chromatography on diethylaminoethanol (DEAE)-cellulose and gel filtration chromatography on Sephadex G25...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28531921/hesperetin-post-treatment-prevents-rat-cardiomyocytes-from-hypoxia-reoxygenation-injury-in-vitro-via-activating-pi3k-akt-signaling-pathway
#8
Shangfei He, Xianbao Wang, Yongkang Zhong, Lu Tang, Ya Zhang, Yuanna Ling, Zhipeng Tan, Pingzhen Yang, Aihua Chen
Hesperidin (HES), a citrus fruit extract, has beneficial effects on various ischemia/reperfusion (I/R) models. Here, we investigated the possible positive effect of hesperetin (HPT), an active metabolite of HES, and identified the potential molecular mechanisms involved in cardiomyocytes H/R-induced injury. To construct the cardiomyocyte model of hypoxia/reoxygenation (H/R) injury, cultured neonatal rat cardiomyocytes were subjected to 3h of hypoxia followed by 3h of reoxygenation. Cell viability and apoptosis were detected...
May 16, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28531805/troxerutin-txn-potentiated-5-fluorouracil-5-fu-treatment-of-human-gastric-cancer-through-suppressing-stat3-nf-%C3%AE%C2%BAb-and-bcl-2-signaling-pathways
#9
Gui-Yun Xu, Xiao-Jun Tang
Gastric cancer still presents a significant problem for public health worldwide. Troxerutin (TXN), a flavonoid present in tea, coffee, cereal grains, and a variety of fruits and vegetables, exhibits various pharmacological and biological activities in vitro and in vivo. We investigated the ability of TXN to reverse the in vitro and in vivo drug resistance of human gastric cancer cells, which were resistant to treatment of 5-Fluorouracil (5-FU). 5-Fu is a pyrimidine analog, which is widely used in the treatment of cancers...
May 19, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28531803/isofraxidin-inhibited-proliferation-and-induced-apoptosis-via-blockage-of-akt-pathway-in-human-colorectal-cancer-cells
#10
Peng Shen, Hong-Gang Wang, Miao-Miao Li, Qian-Yun Ma, Chuan-Wen Zhou, Feng Pan, Rui Xie
BACKGROUND: Isofraxidin (IF), a natural coumarin compound, has been reported to possess anti-cancer activity in human liver cancer. However, whether IF is involved in the regulation of colorectal cancer tumorigenesis and development has been not well elucidated. METHODS: The cell proliferation were assessed by Cell Counting Kit-8 (CCK-8) and colony formation test, respectively. The transwell assays were conducted to estimate cell migration and invasion abilities...
May 19, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28531218/autophagy-inhibitor-facilitates-gefitinib-sensitivity-in-vitro-and-in-vivo-by-activating-mitochondrial-apoptosis-in-triple-negative-breast-cancer
#11
Zhaoyun Liu, Kewen He, Qinghua Ma, Qian Yu, Chenyu Liu, Isabella Ndege, Xinzhao Wang, Zhiyong Yu
Epidermal growth factor receptor (EGFR) is over-expressed in about 50% of Triple negative breast cancers (TNBCs), but EGFR inhibitors have not been effective in treating TNBC patients. Increasing evidence supports that autophagy was related to drug resistance at present. However, the role and the mechanism of autophagy to the treatment of TNBC remain unknown. In the current study, we investigated the effect of autophagy inhibitor to gefitinib (Ge) in TNBC cells in vitro and in nude mice vivo. Our study demonstrated that inhibition of autophagy by 3-Methyladenine or bafilomycin A1 improved Ge's sensitivity to MDA-MB-231 and MDA-MB-468 cells, as evidence from stronger inhibition of cell vitality and colony formation, higher level of G0/G1 arrest and DNA damage, and these effects were verified in nude mice vivo...
2017: PloS One
https://www.readbyqxmd.com/read/28531143/a-4-phenoxyphenol-derivative-exerts-inhibitory-effects-on-human-hepatocellular-carcinoma-cells-through-regulating-autophagy-and-apoptosis-accompanied-by-downregulating-%C3%AE-tubulin-expression
#12
Wen-Tsan Chang, Wangta Liu, Yi-Han Chiu, Bing-Hung Chen, Shih-Chang Chuang, Yen-Chun Chen, Yun-Tzh Hsu, Mei-Jei Lu, Shean-Jaw Chiou, Chon-Kit Chou, Chien-Chih Chiu
Hepatocellular carcinoma (HCC) is a leading cancer worldwide. Advanced HCCs are usually resistant to anticancer drugs, causing unsatisfactory chemotherapy outcomes. In this study, we showed that a 4-phenoxyphenol derivative, 4-[4-(4-hydroxyphenoxy)phenoxy]phenol (4-HPPP), exerts an inhibitory activity against two HCC cell lines, Huh7 and Ha22T. We further investigated the anti-HCC activities of 4-HPPP, including anti-proliferation and induction of apoptosis. Our results showed that higher dosage of 4-HPPP downregulates the expression of α-tubulin and causes nuclear enlargement in both the Huh-7 and Ha22T cell lines...
May 21, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28529245/inhibition-of-nf-%C3%AE%C2%BAb-activation-improves-insulin-resistance-of-l6-cells
#13
Fengyang Xie, Jian Zhu, Baolian Hou, Yuan Wang, Fanzhu Meng, Zhixing Ren, Shuping Ren
To explore the role of NF-κB activation in the development of insulin resistance and investigate whether or not that the inhibition of NF-κB activation by PDTC will improve the insulin resistance of L6 cells exposed to H2O2. L6 cells were treated with H2O2, PDTC or both H2O2 and PDTC for 4 hours. The uptake of glucose with stimulation of insulin, the expression of P38-MAPK, p- P38-MAPK, NF-κBp65, p- NF-κBp65, IRS-1, IRS-2, p-IRS-2, PI3K, IκBα, p- IκBα, caspase-8 and GLUT4, the production of ROS, TNF-α, IL-6 and IL-1β as well as the apoptosis rate of L6 cells were determined and compared in L6 treated with H2O2 alone or both H2O2 and PDTC...
May 20, 2017: Endocrine Journal
https://www.readbyqxmd.com/read/28529240/protective-effects-of-curcumin-on-manganese-induced-bv-2-microglial-cell-death
#14
Euteum Park, Hong Sung Chun
Curcumin, a bioactive component in tumeric, has been shown to exert antioxidant, anti-inflammatory, anticarcinogenic, hepatoprotective, and neuroprotective effects, but the effects of curcumin against manganese (Mn)-mediated neurotoxicity have not been studied. This study examined the protective effects of curcumin on Mn-induced cytotoxicity in BV-2 microglial cells. Curcumin (0.1-10 μM) dose-dependently prevented Mn (250 μM)-induced cell death. Mn-induced mitochondria-related apoptotic characteristics, such as caspase-3 and -9 activation, cytochrome c release, Bax increase, and Bcl-2 decrease, were significantly suppressed by curcumin...
May 20, 2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28528741/in-vitro-immune-toxicity-of-ochratoxin-a-in-porcine-alveolar-macrophages-a-role-for-the-ros-relative-tlr4-myd88-signaling-pathway
#15
Haibin Xu, Shu Hao, Fang Gan, Hong Wang, Jing Xu, Dandan Liu, Kehe Huang
Ochratoxin A (OTA) frequently contaminates a wide variety of food and feeds, inducing immune toxicity of animals. However, the underlying mechanism remains unclear. In the present study, porcine alveolar macrophages (PAMs) cell line 3D4/21 were chosen as a model, and treated by various concentrations of OTA. The results showed that OTA induced immune toxicity of PAMs in a dose-dependent manner, as demonstrated by cell viability, LDH release, Annexin V/PI staining, Bcl-2/Bax mRNA ratio, and pro-inflammatory cytokines expression...
May 18, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28528571/induction-effect-to-apoptosis-by-maitake-polysaccharide
#16
Lei Duan, Xiao-Lu Wu, Fei Zhao, Rong Zeng, Ke-Hu Yang
Polysaccharide extracted from the Maitake mushroom (MP) is considered as a potential anticancer agent. The present study was performed to investigate the cytotoxic effects of MP and vitamin C (VC) alone and in combination on the viability of human neuroglioma M059 K cells in vitro. A combination of MP (1.0 mg/mL) and VC (0.4 mmol/L) led to a 53.10% reduction in cell viability and this treatment induced cell cycle arrest at the G2/M phase, and apoptosis occurred in 38.54% of the cells. Results of Hoechst 33258 staining and Western blot showed apoptotic cells appeared and changes in the expression of apoptosis-related proteins (upregulation of Bax and caspase-3, downregulation of Bcl-2, and activation of poly-(ADP-ribose)-polymerase)...
January 1, 2017: Journal of Evidence-based Complementary & Alternative Medicine
https://www.readbyqxmd.com/read/28528183/effects-of-quercetin-on-proliferation-and-migration-of-human-glioblastoma-u251-cells
#17
Yue Liu, Zhen-Gang Tang, Yi Lin, Xin-Guo Qu, Wei Lv, Guo-Bin Wang, Cai-Li Li
Quercetin is a flavonoid that has been shown to have anti-oxidation, anti-inflammation, anti-allergic, anti-viral, and anti-cancer activities. Here, we examined the effects of quercetin on cell viability, cell cycle progression, and migration in U251 cells, a human glioblastoma cell line. We found that quercetin inhibited cell proliferation after treating cells for 24 (IC50 of 113.65μg/ml) or 48h (IC50 of 48.61μg/ml). Quercetin treatment also induced apoptosis via deregulating the expression of apoptotic genes, including Bax and Bcl-2, and arrested cell cycle at G2/M phases...
May 18, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28528150/long-term-prognosis-of-patients-with-intramural-course-of-coronary-arteries-assessed-with-ct-angiography
#18
Aukelien C Dimitriu-Leen, Alexander R van Rosendael, Jeff M Smit, Tessa van Elst, Nan van Geloven, Teemu Maaniitty, J Wouter Jukema, Victoria Delgado, Arthur J H A Scholte, Antti Saraste, Juhani Knuuti, Jeroen J Bax
OBJECTIVES: The aim of the present study was to evaluate, in low-to-intermediate pre-test probability patients who were referred for coronary computed tomography angiography (CTA) and did not show obstructive coronary artery disease (CAD), whether an intramural course of a coronary artery is associated with worse outcome compared with patients without an intramural course of the coronary arteries. BACKGROUND: The prognostic value of an intramural course of the coronary arteries on coronary CTA in patients without obstructive CAD is not well-known...
May 11, 2017: JACC. Cardiovascular Imaging
https://www.readbyqxmd.com/read/28528146/prognostic-value-of-coronary-ct-angiography-with-selective-pet-perfusion-imaging-in-coronary-artery-disease
#19
Teemu Maaniitty, Iida Stenström, Jeroen J Bax, Valtteri Uusitalo, Heikki Ukkonen, Sami Kajander, Maija Mäki, Antti Saraste, Juhani Knuuti
OBJECTIVES: The purpose of this study was to evaluate the prognostic value of sequential hybrid imaging strategy in which positron emission tomography (PET) perfusion imaging is performed selectively in patients with suspected obstructive coronary artery disease (CAD) on coronary computed tomography angiography (CTA). BACKGROUND: Coronary CTA is an accurate diagnostic test for excluding obstructive CAD. However, the positive predictive value is suboptimal. METHODS: We investigated 864 consecutive symptomatic patients with intermediate probability of CAD who adhered to the sequential imaging approach...
May 15, 2017: JACC. Cardiovascular Imaging
https://www.readbyqxmd.com/read/28528022/direct-effect-of-curcumin-on-porcine-ovarian-cell-functions
#20
Attila Kádasi, Nora Maruniaková, Aneta Štochmaľová, Miroslav Bauer, Roland Grossmann, Abdel Halim Harrath, Adriana Kolesárová, Alexander V Sirotkin
Curcuma longa Linn (L.) is a plant widely used in cooking (in curry powder a.o.) and in folk medicine, but its action on reproductive processes and its possible mechanisms of action remain to be investigated. The objective of this study was to examine the direct effects of curcumin, the major Curcuma longa L. molecule, on basic ovarian cell functions such as proliferation, apoptosis, viability and steroidogenesis. Porcine ovarian granulosa cells were cultured with and without curcumin (at doses of 0, 1, 10 and 100μg/ml of medium)...
May 12, 2017: Animal Reproduction Science
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