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Alum adjuvant

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https://www.readbyqxmd.com/read/28621112/increased-immunogenicity-and-protective-efficacy-of-the-p-aeruginosa-vaccine-in-mice-using-an-alum-and-de-o-acylated-lipooligosaccharide-adjuvant-system
#1
Ji In Ryu, Seo Ri Wui, Ara Ko, Yeon Jeong Lee, Hien Thi Thu Do, Hark Jun Kim, In Moo Rhee, Shin Ae Park, Kwang Sung Kim, Yang Je Cho, Na Gyong Lee
Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen that commonly causes fatal infections in cystic fibrosis and burn patients as well as in patients who are hospitalized or have impaired immune systems. P. aeruginosa infections are difficult to treat due to the high resistance of the pathogen to conventional antibiotics. Despite several efforts, no effective prophylactic vaccines against P. aeruginosa are currently available. In this study, we investigated the activity of the CIA06 adjuvant system, which is comprised of alum and de-O-acylated lipooligosaccharide, on a P...
June 16, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28619071/new-adenovirus-based-vaccine-vectors-targeting-pfs25-elicit-antibodies-that-inhibit-plasmodium-falciparum-transmission
#2
Kathleen A McGuire, Kazutoyo Miura, Christopher M Wiethoff, Kim C Williamson
BACKGROUND: An effective malaria transmission-blocking vaccine (TBV) would be a major advance in the current efforts to eliminate and, ultimately, eradicate malaria. Antibodies against Plasmodium falciparum surface protein, Pfs25, are known to block parasite development in the mosquito vector. However, in initial clinical trials the limited immunogenicity of recombinant Pfs25 protein-in-adjuvant vaccines has been a challenge. METHODS: Novel human adenovirus type 5 (Ad5) vectors were used in heterologous prime boost vaccination strategies to augment the immune response against Pfs25...
June 15, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28600891/spontaneous-food-allergy-in-was-mice-occurs-independent-of-fc%C3%AE%C2%B5ri-mediated-mast-cell-activation
#3
Willem S Lexmond, Jeremy A Goettel, Benjamin F Sallis, Katelyn McCann, Edmond H H M Rings, Erika Jensen-Jarolim, Samuel Nurko, Scott B Snapper, Edda Fiebiger
BACKGROUND: Food allergies are a growing health problem and the development of therapies that prevent disease onset is limited by the lack of adjuvant-free experimental animal models. We compared allergic sensitization in patients with food allergy or Wiskott-Aldrich syndrome (WAS) and defined whether spontaneous disease in Was(-/-) mice recapitulates the pathology of a conventional disease model and/or human food allergy. METHODS: Comparative ImmunoCAP ISAC microarray was performed in patients with food allergy or WAS...
June 10, 2017: Allergy
https://www.readbyqxmd.com/read/28595972/17%C3%AE-ethynylestradiol-alters-the-peritoneal-immune-response-of-gilthead-seabream
#4
N E Gómez González, I Cabas, M C Rodenas, M Arizcun, V Mulero, A García Ayala
17α-Ethynylestradiol (EE2), a synthetic estrogen used in most oral contraceptives pills and hormone replacement therapies, is found in many water bodies, where it can modulate the fish immune response. EE2 acts as an endocrine disruptor in gilthead seabream, Sparus aurata L., a marine teleost fish of great economic value in Mediterranean aquaculture, as it induces hepatic vitellogenin gene (vtg) expression. Moreover, EE2 also alters the capacity of gilthead seabream to appropriately respond to infection although it does not behave as an immunosuppressor...
June 9, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28592561/vaccine-priming-is-restricted-to-draining-lymph-nodes-and-controlled-by-adjuvant-mediated-antigen-uptake
#5
Frank Liang, Gustaf Lindgren, Kerrie J Sandgren, Elizabeth A Thompson, Joseph R Francica, Anja Seubert, Ennio De Gregorio, Susan Barnett, Derek T O'Hagan, Nancy J Sullivan, Richard A Koup, Robert A Seder, Karin Loré
The innate immune mechanisms by which adjuvants enhance the potency and protection of vaccine-induced adaptive immunity are largely unknown. We introduce a model to delineate the steps of how adjuvant-driven innate immune activation leads to priming of vaccine responses using rhesus macaques. Fluorescently labeled HIV-1 envelope glycoprotein (Env) was administered together with the conventional aluminum salt (alum) adjuvant. This was compared to Env given with alum with preabsorbed Toll-like receptor 7 (TLR7) ligand (alum-TLR7) or the emulsion MF59 because they show superiority over alum for qualitatively and quantitatively improved vaccine responses...
June 7, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/28587283/influence-of-hesperidin-on-the-systemic-and-intestinal-rat-immune-response
#6
Mariona Camps-Bossacoma, Àngels Franch, Francisco J Pérez-Cano, Margarida Castell
Polyphenols, widely found in edible plants, influence the immune system. Nevertheless, the immunomodulatory properties of hesperidin, the predominant flavanone in oranges, have not been deeply studied. To establish the effect of hesperidin on in vivo immune response, two different conditions of immune system stimulations in Lewis rats were applied. In the first experimental design, rats were intraperitoneally immunized with ovalbumin (OVA) plus Bordetella pertussis toxin and alum as the adjuvants, and orally given 100 or 200 mg/kg hesperidin...
June 6, 2017: Nutrients
https://www.readbyqxmd.com/read/28576571/nontoxic-outer-membrane-vesicles-efficiently-increase-the-efficacy-of-an-influenza-vaccine-in-mice-and-ferrets
#7
Sang-Mu Shim, Eun-Jung Song, Daesub Song, Tae-Young Lee, Doo-Jin Kim, Jeong-Hyun Nam, Dae Gwin Jeong, Chong-Kil Lee, Sang-Hyun Kim, Jeong-Ki Kim
In this study, we developed a further-modified outer membrane vesicle (fmOMV) from the ΔmsbB/ΔpagP mutant of Escherichia coli transformed with the plasmid, pLpxF, in order to use it as an adjuvant for pandemic H1N1 (pH1N1) influenza vaccine. We evaluated the efficacy of the pH1N1 influenza vaccine containing the fmOMV in animal models as compared to the commercial adjuvants, alum or AddaVax(TM). The fmOMV-adjuvanted pH1N1 influenza vaccine induced a significant increase in the humoral immunity; however, this effect was less than that of the AddaVax(TM)...
May 30, 2017: Vaccine
https://www.readbyqxmd.com/read/28574716/development-of-a-clinically-viable-heroin-vaccine
#8
Paul T Bremer, Joel E Schlosburg, Matthew L Banks, Floyd F Steele, Bin Zhou, Justin L Poklis, Kim D Janda
Heroin is a highly abused opioid and incurs a significant detriment to society worldwide. In an effort to expand the limited pharmacotherapy options for opioid use disorders, a heroin conjugate vaccine was developed through comprehensive evaluation of hapten structure, carrier protein, adjuvant and dosing. Immunization of mice with an optimized heroin-tetanus toxoid (TT) conjugate formulated with adjuvants alum and CpG oligodeoxynucleotide (ODN) generated heroin "immunoantagonism", reducing heroin potency by >15-fold...
June 20, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28560654/newer-vaccines-against-mosquito-borne-diseases
#9
Anju Aggarwal, Neha Garg
Mosquitos are responsible for a number of protozoal and viral diseases. Malaria, dengue, Japanese encephalitis (JE) and chikungunya epidemics occur commonly all over the world, leading to marked mortality and morbidity in children. Zika, Yellow fever and West Nile fever are others requiring prevention. Environmental control and mosquito bite prevention are useful in decreasing the burden of disease but vaccination has been found to be most cost-effective and is the need of the hour. RTS,S/AS01 vaccine is the first malaria vaccine being licensed for use against P...
May 31, 2017: Indian Journal of Pediatrics
https://www.readbyqxmd.com/read/28546072/dendrimer-conjugated-peptide-vaccine-enhances-clearance-of-chlamydia-trachomatis-genital-infection
#10
Ingrid S Ganda, Qian Zhong, Mirabela Hali, Ricardo L C Albuquerque, Francine F Padilha, Sandro R P da Rocha, Judith A Whittum-Hudson
Peptide-based vaccines have emerged in recent years as promising candidates in the prevention of infectious diseases. However, there are many challenges to maintaining in vivo peptide stability and enhancement of peptide immunogenicity to generate protective immunity which enhances clearance of infections. Here, a dendrimer-based carrier system is proposed for peptide-based vaccine delivery, and shows its anti-microbial feasibility in a mouse model of Chlamydia trachomatis. Chlamydiae are the most prevalent sexually transmitted bacteria worldwide, and also the causal agent of trachoma, the leading cause of preventable infectious blindness...
May 22, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28544850/pcpp-adjuvanted-respiratory-syncytial-virus-rsv-sf-subunit-vaccine-self-assembled-supramolecular-complexes-enable-enhanced-immunogenicity-and-protection
#11
Corinne Cayatte, Alexander Marin, Gaurav Manohar Rajani, Kirsten Schneider-Ohrum, Angie Snell Bennett, Jason D Marshall, Alexander K Andrianov
PCPP, a well-defined polyphosphazene macromolecule, has been studied as an immunoadjuvant for a soluble form of the postfusion glycoprotein of respiratory syncytial virus (RSV sF), which is an attractive vaccine candidate for inducing RSV-specific immunity in mice and humans. We demonstrate that RSV sF-PCPP formulations induce high neutralization titers to RSV comparable to alum formulations even at a low PCPP dose and protect animals against viral challenge both in the lung and in the upper respiratory tract...
June 7, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28516208/safety-and-efficiency-of-active-immunization-with-detoxified-antigen-against-scorpion-venom-side-effect-evaluation
#12
Nassrine Bachsais, Lila Boussag-Abib, Fatima Laraba-Djebari
OBJECTIVE: The efficiency and safety of vaccine are the most important properties, however, as any medication, it can induce side effects. This prophylactic therapy could be used to prevent the lethal and pathophysiological effects induced after scorpion envenomation. METHODS: In this study, detoxified venom associated to alum adjuvant (V*alum) is used as a vaccine against scorpion venom for immunization of mice. We evaluate the safety and the inflammatory response of this vaccine...
May 17, 2017: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
https://www.readbyqxmd.com/read/28515346/the-novel-complex-combination-of-alum-cpg-odn-and-hh2-as-adjuvant-in-cancer-vaccine-effectively-suppresses-tumor-growth-in-vivo
#13
Yaomei Tian, Meng Li, Chaoheng Yu, Rui Zhang, Xueyan Zhang, Rong Huang, Lian Lu, Fengjiao Yuan, Yingzi Fan, Bailing Zhou, Ke Men, Heng Xu, Li Yang
Single-component adjuvant is prone to eliciting a specific type of Th1 or Th2 response. So, the development of combinatorial adjuvants inducing a robust mixed Th1/Th2 response is a promising vaccination strategy against cancer. Here, we describe a novel combination of aluminum salts (alum), CpG oligodeoxynucleotide (CpG) and innate defense regulator peptide HH2 for improving anti-tumor immune responses. The CpG-HH2 complex significantly enhanced the production of IFN-γ, TNF-α and IL-1β, promoted the uptake of antigen and strengthened the activation of p38, Erk1/2 and NF-κB in vitro, compared to CpG or HH2 alone...
April 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28501509/designer-outer-membrane-vesicles-as-immunomodulatory-systems-reprogramming-bacteria-for-vaccine-delivery
#14
Yehou M D Gnopo, Hannah C Watkins, Taylor C Stevenson, Matthew P DeLisa, David Putnam
Vaccines often require adjuvants to be effective. Traditional adjuvants, like alum, activate the immune response but in an uncontrolled way. Newer adjuvants help to direct the immune response in a more coordinated fashion. Here, we review the opportunity to use the outer membrane vesicles (OMVs) of bacteria as a way to modulate the immune response toward making more effective vaccines. This review outlines the different types of OMVs that have been investigated for vaccine delivery and how they are produced...
May 10, 2017: Advanced Drug Delivery Reviews
https://www.readbyqxmd.com/read/28487847/recombinant-tghsp70-immunization-protects-against-toxoplasma-gondii-brain-cyst-formation-by-enhancing-inducible-nitric-oxide-expression
#15
Paulo Czarnewski, Ester C B Araújo, Mário C Oliveira, Tiago W P Mineo, Neide M Silva
Toxoplasma gondii is known to cause congenital infection in humans and animals and severe disease in immunocompromised individuals; consequently development of vaccines against the parasite is highly necessary. Under stress conditions, T. gondii expresses the highly immunogenic heat shock protein 70 (TgHSP70). Here, we assessed the protective efficacy of rTgHSP70 immunization combined with Alum in oral ME-49 T. gondii infection and the mechanisms involved on it. It was observed that immunized mice with rTgHSP70 or rTgHSP70 adsorbed in Alum presented a significantly reduced number of cysts in the brain that was associated with increased iNOS+ cell numbers in the organ, irrespective the use of the adjuvant...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28481687/a-trivalent-subunit-antigen-glycoprotein-vaccine-as-immunotherapy-for-genital-herpes-in-the-guinea-pig-genital-infection-model
#16
Sita Awasthi, Lauren M Hook, Carolyn E Shaw, Harvey M Friedman
An estimated 417 million people worldwide ages 15 to 49 are infected with herpes simplex virus type 2 (HSV-2), the most common cause of genital ulcer disease. Some individuals experience frequent recurrences of genital lesions, while others only have subclinical infection, yet all risk transmitting infection to their intimate partners. A vaccine was developed that prevents shingles, which is a recurrent infection caused by varicella-zoster virus (VZV), a closely related member of the Herpesviridae family. The success of the VZV vaccine has stimulated renewed interest in a therapeutic vaccine for genital herpes...
May 8, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28479181/carbohydrate-fatty-acid-monosulphate-esters-are-safe-and-effective-adjuvants-for-humoral-responses
#17
Luuk A Th Hilgers, Peter Paul L I Platenburg, Jeffrey Bajramovic, Jennifer Veth, Robert Sauerwein, Will Roeffen, Marie Pohl, Geert van Amerongen, Koert J Stittelaar, Johannes F van den Bosch
Carbohydrate fatty acid sulphate esters (CFASEs) formulated in a squalane-in-water emulsion are effective adjuvants for humoral responses to a wide range of antigens in various animal species but rise in body temperature and local reactions albeit mild or minimal hampers application in humans. In rabbits, body temperature increased 1°C one day after intramuscular (IM) injection, which returned to normal during the next day. The effect increased with increasing dose of CFASE but not with the number of injections (up to 5)...
May 31, 2017: Vaccine
https://www.readbyqxmd.com/read/28479180/accelerated-and-long-term-stability-study-of-pfs25-epa-conjugates-adjuvanted-with-alhydrogel%C3%A2
#18
Daming Zhu, Yimin Wu, Holly McClellan, Weili Dai, Kelly Rausch, Dominique Jones, Joan Aebig, Emma Barnafo, Brandi Butler, Lynn Lambert, David L Narum, Patrick E Duffy
Pfs25, a Plasmodium falciparum surface protein expressed during zygote and ookinete stages in infected mosquitoes, is a lead transmission-blocking vaccine candidate against falciparum malaria. To enhance immunogenicity, recombinant Pfs25 was chemically conjugated to recombinant nontoxic Pseudomonas aeruginosa ExoProtein A (rEPA) in conformance with current good manufacturing practices (cGMP), and formulated with the alum adjuvant Alhydrogel. In order to meet the regulatory requirements for a phase 1 human clinical trial, the vaccine product was extensively evaluated for stability at an initial time point and through the clinical trial period annually...
May 31, 2017: Vaccine
https://www.readbyqxmd.com/read/28468322/virus-like-particle-vlp-plus-microcrystalline-tyrosine-mct-adjuvants-enhance-vaccine-efficacy-improving-t-and-b-cell-immunogenicity-and-protection-against-plasmodium-berghei-vivax
#19
Gustavo Cabral-Miranda, Matthew D Heath, Mona O Mohsen, Ariane C Gomes, Paul Engeroff, Amy Flaxman, Fabiana M S Leoratti, Aadil El-Turabi, Arturo Reyes-Sandoval, Murray A Skinner, Matthias F Kramer, Martin F Bachmann
Vaccination is the most effective prophylactic tool against infectious diseases. Despite continued efforts to control malaria, the disease still generally represents a significant unmet medical need. Microcrystalline tyrosine (MCT) is a well described depot used in licensed allergy immunotherapy products and in clinical development. However, its proof of concept in prophylactic vaccines has only recently been explored. MCT has never been used in combination with virus-like particles (VLPs), which are considered to be one of the most potent inducers of cellular and humoral immune responses in mice and humans...
May 2, 2017: Vaccines
https://www.readbyqxmd.com/read/28456624/fusion-proteins-of-flagellin-and-the-major-birch-pollen-allergen-bet-v-1-show-enhanced-immunogenicity-reduced-allergenicity-and-intrinsic-adjuvanticity
#20
Claudia Kitzmüller, Julia Kalser, Sonja Mutschlechner, Michael Hauser, Gerhard J Zlabinger, Fatima Ferreira, Barbara Bohle
BACKGROUND: Recombinant fusion proteins of flagellin and antigens have been demonstrated to induce strong innate and adaptive immune responses. Such fusion proteins may enhance the efficacy of allergen-specific immunotherapy (AIT). OBJECTIVE: To characterize different fusion proteins of flagellin and the major birch pollen allergen Bet v 1 for suitability as allergy vaccines. METHODS: A truncated version of flagellin (NtCFlg) was genetically fused to the N- or C-terminus of Bet v 1...
April 26, 2017: Journal of Allergy and Clinical Immunology
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