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Alum adjuvant

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https://www.readbyqxmd.com/read/28322289/poly-%C3%AE-glutamic-acid-chitosan-nanogel-greatly-enhances-the-efficacy-and-heterosubtypic-cross-reactivity-of-h1n1-pandemic-influenza-vaccine
#1
Jihyun Yang, Sang-Mu Shim, Thi Quyen Nguyen, Eun-Ha Kim, Kwang Kim, Yong Taik Lim, Moon-Hee Sung, Richard Webby, Haryoung Poo
In 2009, the global outbreak of an influenza pandemic emphasized the need for an effective vaccine adjuvant. In this study, we examined the efficacy of poly-γ-glutamic acid/chitosan (PC) nanogel as an adjuvant for the influenza vaccine. PC nanogel significantly enhanced antigen-specific cross-presentation and cytotoxic T lymphocyte (CTL) activity. Compared with alum, the protective efficacy of the pandemic H1N1 influenza (pH1N1) vaccine was substantially increased by PC nanogel, with increased hemagglutination-inhibition titers, CTL activity, and earlier virus clearance after homologous and heterosubtypic [A/Philippines/2/82 (H3N2)] virus challenges...
March 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28316602/toll-like-receptor-ligand-based-vaccine-adjuvants-require-intact-myd88-signaling-in-antigen-presenting-cells-for-germinal-center-formation-and-antibody-production
#2
Munir M Mosaheb, Michael L Reiser, Lee M Wetzler
Vaccines are critical in the fight against infectious diseases, and immune-stimulating adjuvants are essential for enhancing vaccine efficacy. However, the precise mechanisms of action of most adjuvants are unknown. There is an urgent need for customized and adjuvant formulated vaccines against immune evading pathogens that remain a risk today. Understanding the specific role of various cell types in adjuvant-induced protective immune responses is vital for an effective vaccine design. We have investigated the role of cell-specific MyD88 signaling in vaccine adjuvant activity in vivo, using Neisserial porin B (PorB), a TLR2 ligand-based adjuvant, compared with an endosomal TLR9 ligand (CpG) and toll-like receptor (TLR)-independent (alum, MF59) adjuvants...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28295241/indirect-effects-of-immunological-tolerance-to-a-regular-dietary-protein-reduce-cutaneous-scar-formation
#3
Thiago Anselmo Cantaruti, Raquel Alves Costa, Kênia Soares de Souza, Nelson Monteiro Vaz, Cláudia Rocha Carvalho
Oral tolerance refers to the specific inhibition of immune responsiveness to T-cell dependent antigens contacted by the oral route before parenteral immunization. Oral tolerance to one protein does not inhibit immune responses to other unrelated proteins, but parenteral injection of tolerated antigens plus adjuvant into tolerant, but not normal mice inhibits immune responses to antigens injected concomitantly or soon thereafter. The inhibitory effect triggered by parenteral injection of tolerated proteins is known as bystander suppression or indirect effects of oral tolerance...
March 12, 2017: Immunology
https://www.readbyqxmd.com/read/28289411/immunogenicity-of-a-bivalent-adjuvanted-glycoconjugate-vaccine-against-salmonella-typhimurium-and-salmonella-enteritidis
#4
Fabio Fiorino, Simona Rondini, Francesca Micoli, Luisa Lanzilao, Renzo Alfini, Francesca Mancini, Calman A MacLennan, Donata Medaglini
Salmonella enterica serovars Typhimurium and Enteritidis are the predominant causes of invasive non-typhoidal Salmonella (iNTS) disease. Considering the co-endemicity of S. Typhimurium and S. Enteritidis, a bivalent vaccine formulation against both pathogens is necessary for protection against iNTS disease, thus investigation of glycoconjugate combination is required. In the present work, we investigated the immune responses induced by S. Typhimurium and S. Enteritidis monovalent and bivalent glycoconjugate vaccines adjuvanted with aluminum hydroxide (alum) only or in combination with cytosine-phosphorothioate-guanine oligodeoxynucleotide (CpG)...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28288639/rhesus-macaque-and-mouse-models-for-down-selecting-circumsporozoite-protein-based-malaria-vaccines-differ-significantly-in-immunogenicity-and-functional-outcomes
#5
Timothy W Phares, Anthony D May, Christopher J Genito, Nathan A Hoyt, Farhat A Khan, Michael D Porter, Margot DeBot, Norman C Waters, Philippe Saudan, Sheetij Dutta
BACKGROUND: Non-human primates, such as the rhesus macaques, are the preferred model for down-selecting human malaria vaccine formulations, but the rhesus model is expensive and does not allow for direct efficacy testing of human malaria vaccines. Transgenic rodent parasites expressing genes of human Plasmodium are now routinely used for efficacy studies of human malaria vaccines. Mice have however rarely predicted success in human malaria trials and there is scepticism whether mouse studies alone are sufficient to move a vaccine candidate into the clinic...
March 13, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28267643/alum-adjuvanted-rabies-dna-vaccine-confers-80-protection-against-lethal-50-ld50-rabies-challenge-virus-standard-strain
#6
Rajni Garg, Manpreet Kaur, Ankur Saxena, Rajendra Prasad, Rakesh Bhatnagar
Rabies is a serious concern world-wide. Despite availability of rabies vaccines for long; their efficacy, safety, availability and cost effectiveness has been a tremendous issue. This calls for improvement of rabies vaccination strategies. DNA vaccination has immense potential in this regard. The DNA vaccine pgp.LAMP-1 conferred 60% protection to BALB/c mice against 20 LD50 rabies challenge virus standard (CVS) strain challenge. Upon supplementation with Emulsigen-D, the vaccine formulation conferred complete protection against lethal challenge...
March 3, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28245781/endogenous-and-exogenous-natural-adjuvants-for-vaccine-development
#7
Azam Bolhassani, Somayeh Talebi, Ali Anvar
Various adjuvants are usually co-injected with an antigen for stimulation of effective immune responses. Their mechanisms of action in vaccine development include: a) enhancement of the total antibody titers; b) reduction of the antigen dose; c) induction of potent cell-mediated immunity; d) increase in the speed and duration of the protective response; e) stimulation of mucosal immunity; and f) cross-protection. Up to now, different exogenous adjuvants have been identified to boost immune responses including inorganic compounds (e...
February 28, 2017: Mini Reviews in Medicinal Chemistry
https://www.readbyqxmd.com/read/28228587/prophylactic-herpes-simplex-virus-2-hsv-2-vaccines-adjuvanted-with-stable-emulsion-and-toll-like-receptor-9-agonist-induce-a-robust-hsv-2-specific-cell-mediated-immune-response-protect-against-symptomatic-disease-and-reduce-the-latent-viral-reservoir
#8
Michael T Hensel, Jason D Marshall, Michael R Dorwart, Darren S Heeke, Eileen Rao, Padmaja Tummala, Li Yu, Gary H Cohen, Roselyn J Eisenberg, Derek D Sloan
Several prophylactic vaccines targeting HSV-2 have failed in the clinic to demonstrate a sustained depression in viral shedding or protection from recurrences. Although these vaccines have generated high titers of neutralizing antibodies, their induction of robust CD8 T cells has largely been unreported, even though evidence for the importance of HSV-2 antigen-specific CD8 T cells is mounting in animal models and in translational studies involving subjects with active HSV-2-specific immune responses. We developed a subunit vaccine composed of the neutralizing antibody (nAb) targets gD and gB, the novel T cell antigen and tegument protein UL40, and we compared this to a whole-inactivated virus vaccine (FI-HSV-2)...
February 22, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28222998/dynamics-of-apc-recruitment-at-the-site-of-injection-following-injection-of-vaccine-adjuvants
#9
Susan van Aalst, Irene Stephanie Ludwig, Peter Johannes Sylvester van Kooten, Ruurd van der Zee, Willem van Eden, Femke Broere
Vaccines often contain adjuvants to strengthen the response to the vaccine antigen. However, their modes of action at the site of injection (SOI) are poorly understood. Therefore, we assessed the local effects of adjuvant on the innate immune system in mice. We investigated the safe, widely used adjuvants MF59 and aluminum hydroxide (alum), as well as trehalose-6,6'-dibehenate (TDB), Complete Freund's Adjuvant (CFA) and the Toll-Like-Receptor-ligands lipopolysaccharide (LPS) and Pam3CysSerLys4 (Pam3CSK4). We assessed muscle immune cell infiltration after adjuvant injection and observed 16h post immunization (hpi) an increased influx with CFA, MF59 and TDB, but not with alum, LPS or Pam3CSK4...
February 17, 2017: Vaccine
https://www.readbyqxmd.com/read/28220116/cpg-odn-shapes-alum-adjuvant-activity-signaling-via-myd88-and-il-10
#10
Luciana Mirotti, Ricardo Wesley Alberca Custódio, Eliane Gomes, Florencia Rammauro, Eliseu Frank de Araujo, Vera Lucia Garcia Calich, Momtchilo Russo
Aluminum-containing adjuvants usually referred as Alum are considered as T helper type-2 (Th2) adjuvants, while agonists of toll-like receptors (TLRs) are viewed as adjuvants that favor Th1/Th17 immunity. Alum has been used in numerous vaccine formulations; however, its undesired pro-Th2 adjuvant activity constitutes a caveat for Alum-based vaccines. Combining Alum with TLR-dependent, pro-Th1/Th17 adjuvants might dampen the pro-Th2 activity and improve the effectiveness of vaccine formulations. Here, using the ovalbumin (OVA) model of allergic lung inflammation, we found that sensitization with the synthetic TLR9 agonist, which is composed of oligodeoxynucleotides containing CpG motifs adsorbed to Alum, inhibited the development of OVA-induced lung allergic Th2 responses without shifting toward a Th1 pattern...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28194450/assessment-of-the-adjuvant-activity-of-mesoporous-silica-nanoparticles-in-recombinant-mycoplasma-hyopneumoniae-antigen-vaccines
#11
Veridiana Gomes Virginio, Natalia Costantin Bandeira, Fernanda Munhoz Dos Anjos Leal, Marcelo Lancellotti, Arnaldo Zaha, Henrique Bunselmeyer Ferreira
The adjuvant potential of two mesoporous silica nanoparticles (MSNs), SBa-15 and SBa-16, was assessed in combination with a recombinant HSP70 surface polypeptide domain from Mycoplasma hyopneumoniae, the etiological agent of porcine enzootic pneumonia (PEP). The recombinant antigen (HSP70212-600), previously shown as immunogenic in formulation with classic adjuvants, was used to immunize BALB/c mice in combination with SBa-15 or SBa-16 MSNs, and the effects obtained with these formulations were compared to those obtained with alum, the adjuvant traditionally used in anti-PEP bacterins...
January 2017: Heliyon
https://www.readbyqxmd.com/read/28183282/enhanced-and-long-term-immunogenicity-of-a-her-2-neu-multi-epitope-vaccine-conjugated-to-the-carrier-crm197-in-conjunction-with-the-adjuvant-montanide
#12
Joshua Tobias, Joanna Jasinska, Karin Baier, Michael Kundi, Nicholas Ede, Christoph Zielinski, Ursula Wiedermann
BACKGROUND: We previously identified three short single peptides (P4, P6 and P7) representing different B-cell epitopes on the extracellular domain of Her-2/neu for a vaccine that was tested in a phase-I clinical trial. Here we describe the improvement of the multi peptide vaccine by fusing the single peptides to a hybrid peptide P467. METHODS: After coupling to either virosomes or to diphtheria toxoid CRM197 (CRM), the hybrid peptide was tested in different concentrations in combination with either Montanide or Aluminium hydroxide (Alum) in preclinical studies...
February 9, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28161462/immune-response-and-protective-profile-elicited-by-a-multi-epitope-chimeric-protein-derived-from-leptospira-interrogans
#13
Luis G V Fernandes, Aline F Teixeira, Antonio F S Filho, Gisele O Souza, Silvio A Vasconcellos, Marcos B Heinemann, Eliete C Romero, Ana L T O Nascimento
INTRODUCTION: Pathogenic Leptospira is the causative agent of leptospirosis, a widely disseminated disease of human and veterinary concern. The development of vaccines that elicit cross-protective immunity through multiple leptospiral serovars has long been pursued. The aim of this study was to develop a novel chimeric multi-epitope fusion antigen, containing sequences of previously studied outer membrane proteins (OMPs) of Leptospira. METHODS: The chimeric protein was designed based on the amino acid sequences of the LigA, Mce, Lsa45, OmpL1, and LipL41 proteins, cloned into pAE vector, the protein expressed in Escherichia coli, and its immune response evaluated in the hamster infection model...
February 1, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28149525/self-assembled-peg-b-pdpa-b-pgem-copolymer-nanoparticles-as-protein-antigen-delivery-vehicles-to-dendritic-cells-preparation-characterization-and-cellular-uptake
#14
Pan Li, Junhui Zhou, Pingsheng Huang, Chuangnian Zhang, Weiwei Wang, Chen Li, Deling Kong
Antigen uptake by dendritic cells (DCs) is a key step for initiating antigen-specific T cell immunity. In the present study, novel synthetic polymeric nanoparticles were prepared as antigen delivery vehicles to improve the antigen uptake by DCs. Well-defined cationic and acid-responsive copolymers, monomethoxy poly(ethylene glycol)-block-poly(2-(diisopropyl amino) ethyl methacrylate)-block-poly(2-(guanidyl) ethyl methacrylate) (mPEG-b-PDPA-b-PGEM, PEDG) were synthesized by reversible addition-fragmentation chain transfer polymerization of 2-(diisopropylamino)ethyl methacrylate) and N-(tert-butoxycarbonyl) amino ethyl methacrylate monomers, followed by deprotection of tert-butyl protective groups and guanidinylation of obtained primary amines...
February 2017: Regenerative Biomaterials
https://www.readbyqxmd.com/read/28148790/immunization-with-low-doses-of-recombinant-post-fusion-or-pre-fusion-rsv-f-primes-for-vaccine-enhanced-disease-in-the-cotton-rat-model-independent-of-the-presence-of-a-th1-biasing-gla-se-or-th2-biasing-alum-adjuvant
#15
Kirsten Schneider-Ohrum, Corinne Cayatte, Angie Snell Bennett, Gaurav Manohar Rajani, Patrick McTamney, Krystal Nacel, Leigh Hostetler, Lily Cheng, Kuishu Ren, Terrence O'Day, Gregory A Prince, Michael P McCarthy
Respiratory syncytial virus (RSV) infection of children previously immunized with a non-live, formalin-inactivated (FI)-RSV vaccine was associated with serious enhanced respiratory disease (ERD). Consequently, detailed studies of potential ERD are a critical step in the development of non-live RSV vaccines targeting RSV-naïve children and infants. The fusion glycoprotein (F) of RSV in either its post- or pre-fusion conformation is a target for neutralizing antibodies and therefore an attractive antigen candidate for a pediatric RSV subunit vaccine...
February 1, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28117172/skin-vaccination-via-fractional-infrared-laser-ablation-optimization-of-laser-parameters-and-adjuvantation
#16
Sandra Scheiblhofer, Anna Strobl, Veronika Hoepflinger, Theresa Thalhamer, Martin Steiner, Josef Thalhamer, Richard Weiss
BACKGROUND: Methods to deliver an antigen into the skin in a painless, defined, and reproducible manner are essential for transcutaneous immunization (TCI). Here, we employed an ablative fractional infrared laser (P.L.E.A.S.E. Professional) to introduce clinically relevant vaccines into the skin. To elicit the highest possible antibody titers with this system, we optimized different laser parameters, such as fluence and pore number per area, and tested various adjuvants. METHODS: BALB/c mice were immunized with Hepatitis B surface antigen (HBsAg) by laser-microporation...
January 20, 2017: Vaccine
https://www.readbyqxmd.com/read/28096968/a-novel-atheroprotective-role-of-mf59-like-adjuvant-when-co-administered-with-cetp-vaccine-in-rabbit-model-of-atherosclerosis
#17
Tamara Aghebati, Amir Hooshang Mohammadpour, Mohammad Afshar, Mahmoud Reza Jaafari, Khalil Abnous, Saeed Nazemi, Sobhan Issazadeh, Saeed Hashemzadeh, Mohammad Zare, Ali Badiee
OBJECTIVES: In this study, for the first time, MF59 adjuvant was used to develop a cholesteryl ester transfer protein (CETP) vaccine. The efficacy of the vaccine was compared with the efficacy of CETP vaccine formulated with Alum/CpG, the formulation that its immunogenicity has been already demonstrated in rabbit and mice. MATERIALS AND METHODS: Tetanus toxoid- CETP peptide (TT-CETP) was mixed with Alum/CpG or MF59-like and administered subcutaneously for total five times in rabbit model of atherosclerosis...
December 2016: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28089548/nanoformulation-of-synergistic-tlr-ligands-to-enhance-vaccination-against-entamoeba-histolytica
#18
Mayuresh M Abhyankar, Zannatun Noor, Mark A Tomai, James Elvecrog, Christopher B Fox, William A Petri
Diarrheal infectious diseases represent a major cause of global morbidity and mortality. There is an urgent need for vaccines against diarrheal pathogens, especially parasites. Modern subunit vaccines rely on combining a highly purified antigen with an adjuvant to increase their efficacy. In the present study, we evaluated the ability of a nanoliposome adjuvant system to trigger a strong mucosal immune response to the Entamoeba histolytica Gal/GalNAc lectin LecA antigen. CBA/J mice were immunized with alum, emulsion or liposome based formulations containing synthetic TLR agonists...
February 7, 2017: Vaccine
https://www.readbyqxmd.com/read/28056837/vaccination-with-toxofilin-dna-in-combination-with-an-alum-monophosphoryl-lipid-a-mixed-adjuvant-induces-significant-protective-immunity-against-toxoplasma-gondii
#19
Pengxia Song, Shenyi He, Aihua Zhou, Gang Lv, Jingjing Guo, Jian Zhou, Yali Han, Huaiyu Zhou, Zhen Hao, Hua Cong
BACKGROUND: A widely prevalent disease, toxoplasmosis poses serious health threats to both humans and animals; therefore, development of an ideal DNA vaccine against Toxoplasma gondii is needed eagerly. The purpose of the present study is to assess the protective efficacy of a DNA vaccine encoding the T. gondii toxofilin gene (pEGFP-toxofilin). In addition, toxofilin DNA vaccine combined with the individual adjuvants, alum or monophosphoryl lipid A (MPLA), or a mixture of alum-MPLA adjuvant were screened for their ability to enhance antibody responses...
January 5, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28043381/characterization-and-vaccine-potential-of-fasciola-gigantica-saposin-like-protein-1-sap-1
#20
Pornanan Kueakhai, Narin Changklungmoa, Pinkamon Waseewiwat, Thanaporn Thanasinpaiboon, Werachon Cheukamud, Pannigan Chaichanasak, Prasert Sobhon
The recombinant Fasciola gigantica Saposin-like protien-1 (rFgSAP-1) was cloned by polymerase chain reaction (PCR) from NEJ cDNA, expressed in Escherichia coli BL21 (DE3) and used for production of a polyclonal antibody in rabbits (anti-rFgSAP-1). By immunoblotting and immunohistochemistry, rabbit IgG anti-rFgSAP-1 reacted with rFgSAP-1 at a molecular weight 12kDa, but not with rFgSAP-2. The rFgSAP-1 reacted with antisera from mouse infected with F. gigantica metacercariae collected at 2, 4, and 6 weeks after infection...
January 15, 2017: Veterinary Parasitology
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