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Xingkang Jiang, Yue Chen, E Du, Kuo Yang, Zhihong Zhang, Shiyong Qi, Yong Xu
Although increasing evidence demonstrated that deregulation of mircoRNA-503 (miRNA-503) contributes to tumorigenesis, little is known about the biological role and intrinsic regulatory mechanisms of miR-503 in prostate cancer (PCa). In present study, we found that miR-503 was significantly downregulated in advanced PCa tissues and cell lines. Downregulation of miR-503 was strongly associated with aggressive clinical-pathological features and poor prognosis in PCa patients. Ectopic expression of miR-503 significantly inhibited tumor cells growth, cell migration and invasion in vitro and in vivo...
September 2016: Cellular Signalling
Hao Shi, Jian Chen, Yuncheng Li, Guojun Li, Rong Zhong, Dandan Du, Ruiwei Meng, Weijia Kong, Meixia Lu
The 5-year survival rate of patients with head and neck squamous cell carcinoma (HNSCC) was only 40%-50%. To investigate the prognostic and predictive value of specific mircoRNAs (miRNAs) in HNSCC. We identified 19 miRNAs associated with over survival (OS) of patients with HNSCC in different clinical classes between 492 HNSCC tissues and 44 normal tissues from The Cancer Genome Atlas (TCGA) dataset. A signature of six miRNAs was identified by the supervised principal component method in the training set. The AUC of the ROC curve for the six microRNA signature predicting 5-year survival was 0...
April 19, 2016: Oncotarget
Rui Yang, Liang Yi, Zhen Dong, Qing Ouyang, Ji Zhou, Yi Pang, Yanan Wu, Lunshan Xu, Hongjuan Cui
Tigecycline is a broad-spectrum, first-in-class glycylcycline antibiotic currently used to treat complicated skin infections and community-acquired pneumonia. However, there is accumulating evidence showing that tigecycline has anticancer properties. In this study, we found tigecycline could inhibit cell proliferation by inducing cell-cycle arrest, but not apoptosis in glioma. To find the underlying mechanism of how tigecycline inhibits cell proliferation, the expression of miRNAs, which were related to regulating cell-cycle progression, was detected with miRNA assay...
March 2016: Molecular Cancer Therapeutics
Ling Lin, Baochun Lu, Jianhua Yu, Wenguang Liu, Aijin Zhou
BACKGROUND AND OBJECTIVE: Circulating microRNAs (miRNAs) are known as potential noninvasive biomarkers for cancers. Overexpression of mircoRNA-224 (miR-224) has been reported in hepatocellular carcinoma (HCC), so the aim of this study was to determine the value of serum miR-224 in diagnosis of HCC at early stage. METHODS: Three hundred and thirty-five subjects including early-stage HCC, liver cirrhosis (LC), chronic hepatitis B (CHB) and healthy controls (HC) were enrolled in two cohorts...
September 2016: Clinics and Research in Hepatology and Gastroenterology
Lihua Yang, Jie Yang, Jingqiu Li, Xingkai Shen, Yanping Le, Chengwei Zhou, Shaomin Wang, Shun Zhang, Dazhi Xu, Zhaohui Gong
Understanding the molecular mechanism by which epithelial mesenchymal transition (EMT)-mediated cancer metastasis and how microRNA (miRNA) regulates lung cancer progression via Twist1-activated EMT may provide potential therapeutic targets for cancer therapy. Here we found that miR-33a, an intronic miRNA located within the sterol regulatory element-binding protein 2 (SREBP-2) gene, is expressed at low levels in metastatic non-small cell lung cancer (NSCLC) cells and is inversely correlated with Twist1 expression...
2015: Scientific Reports
Q Ji, Q Jiang, W Yan, X Li, Y Zhang, P Meng, M Shao, L Chen, H Zhu, N Tian
AIM: The aim of this study was to investigate microRNA expression in ST segment elevation myocardial infarction (STEMI) and non-ST segment elevation myocardial infarction (NSTEMI), and compare their diagnostic values. METHODS: We assessed 77 STEMI and 21 NSTEMI patients admitted to the Department of Cardiology in Nanjing First Hospital from October 2013 to August 2014, and 23 healthy volunteers were selected at the same time. Serum miR-133a, miR-133b and miR-499-5p were measured by using quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR), and the concentration of serum cTnI was measured by enzyme-linked immunosorbent assay (ELISA) methods...
October 2015: Minerva Cardioangiologica
Axel Freischmidt, Kathrin Müller, Lisa Zondler, Patrick Weydt, Benjamin Mayer, Christine A F von Arnim, Annemarie Hübers, Johannes Dorst, Markus Otto, Karlheinz Holzmann, Albert C Ludolph, Karin M Danzer, Jochen H Weishaupt
MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression and specific mircoRNA "fingerprints" are thought to contribute to and/or reflect certain disease conditions. Recently, we identified surprisingly homogeneous signatures of circulating miRNAs in the serum of familial amyotrophic lateral sclerosis (ALS) patients, which were already present in presymptomatic carriers of ALS gene mutations. Here, we characterize circulating miRNAs in the serum of sporadic ALS patients. We show that, in contrast to familial ALS, miRNA signatures of sporadic ALS are highly heterogeneous suggesting a number of different etiologies...
September 2015: Neurobiology of Aging
X Fan, Z Mao, D He, C Liao, X Jiang, N Lei, B Hu, X Wang, Z Li, Y Lin, X Gou, Y Zhu, H Wang
INTRODUCTION: Long-acting somatostatin analogs (SSAs) are most widely used to treat growth hormone (GH)-secreting pituitary adenoma. However, approximately 30 % of treated patients show resistance to SSAs, which may be associated with the reduction of somatostatin receptor subtype 2 (SSTR2) mRNA and protein expression. MATERIALS AND METHODS: The present study used immunohistochemistry to detect the expression of SSTR2 and SSTR5 in twenty human GH-secreting adenoma samples treated with SSAs and seven normal pituitary samples...
October 2015: Journal of Endocrinological Investigation
Kaladhar B Reddy
In recent years, there has been a tremendous and growing interest among researchers to investigate the role of mircoRNA (miRNA) in normal cellular as well as in disease processes. miRNAs are a family of small non-coding RNAs which were reported to regulate the expression of various oncogenes or tumor suppressor genes. The expression profiling of miRNAs has already entered into cancer clinics as diagnostic and prognostic biomarkers to assess tumor initiation, progression and response to treatment in cancer patients...
2015: Cancer Cell International
Johannes A Veit, Kathrin Scheckenbach, Patrick J Schuler, Simon Laban, Paul Severin Wiggenhauser, Severin P Wiggenhauser, Julia Thierauf, Jens P Klussmann, Thomas K Hoffmann
BACKGROUND/AIM: Head and neck adenoid cystic carcinoma (HNACC) is a rare malignancy of the salivary glands with a tendency to metastasize in lung or liver without lymph node involvement, whereas squamous cell carcinoma (HNSCC) preferentially metastasizes to locoregional lymph nodes. The expression patterns of microRNA, a class of small non-coding RNA transcripts, involved in gene regulation and various developmental processes, could be of influence during the metastatic process. The aim of the present study was to compare mircoRNA expression patterns of HNACC and HNSCC...
March 2015: Anticancer Research
Peng Song, Weizhi Wang, Guoquan Tao, Haiyan Chu, Meilin Wang, Dongmei Wu, Na Tong, Weida Gong, Jianwei Zhou, Zhengdong Zhang, Baolin Wang, Haixia Zhu, Qinghong Zhao
Single nucleotide polymorphisms (SNPs) in the 3'-untranslated regions (UTRs) targeted by putative mircoRNAs (miRNAs) could influence the susceptibility of cancer. Recently, miR-29c has been reported to be down-regulated in gastric cancer (GC) and serve as a tumor suppressor that regulated tumor progression. The present study was aimed at investigating whether the miR-29c binding site SNPs within the 3'-UTRs of target genes affected the gastric cancer risk. Using bioinformatics tools, we chose three SNPs (IGHMBP2 rs3750980, LAMTOR3 rs11944405 and WWOX rs2288035) located in miR-29c binding sites...
February 2015: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Haiwei Wu, Shengyun Huang, Dongsheng Zhang
Autophagy is a major intracellular pathway involving in the degradation and recycling of cytosolic material, including organelles, proteins, and ribosomes. Autophagy is commonly active in tumor cells and could be induced by stress conditions such as hypoxia, nutrient depletion and anticancer therapy. Increasing evidence supports the role of autophagy in modulating cancer behavior in head and neck squamous cell carcinoma (HNSCC). Despite recent advances in surgery combined with chemotherapy and radiotherapy, the survival rate of patients with HNSCC has not been improved substantially...
February 2015: Pathology, Research and Practice
Jae Youn Cheong, Hyoung Doo Shin, Sung Won Cho, Yoon Jun Kim
MicroRNA polymorphisms may be associated with carcinogenesis or immunopathogenesis of infection. We evaluated whether the mircoRNA-604 (miR-604) polymorphism can affect the persistence of hepatitis B virus (HBV) infection, and the development to hepatocellular carcinoma (HCC) in patients with chronic HBV infection. A total of 1,439 subjects, who have either past or present HBV infection, were enrolled and divided into four groups (spontaneous recovery, chronic HBV carrier without cirrhosis, liver cirrhosis and HCC)...
November 2014: Journal of Korean Medical Science
Ning Kong, Xiaohe Lu, Bin Li
BACKGROUND: Retinal ganglion cells (RGCs) are preferentially lost in glaucoma or optic neuritis. In the present study, we investigated the protective effect of mircoRNA 100 (miR-100) against oxidative stress induced apoptosis in RGC-5 cells. RESULTS: Rat RGC-5 cells were cultured in plates and H2O2 was added to induce oxidative stress. TUNEL assay and qRT-PCR showed H2O2 induced apoptosis and up-regulated miR-100 in a dose-dependent manner in RGC-5 cells. Conversely, lentiviral-mediated miR-100 down-regulation protected H2O2 induced apoptosis, promoted neurite growth and activated AKT/ERK and TrkB pathways through phosphorylation...
2014: BMC Molecular Biology
Peng Song, Haixia Zhu, Dong Zhang, Haiyan Chu, Dongmei Wu, Meiyun Kang, Meilin Wang, Weida Gong, Jianwei Zhou, Zhengdong Zhang, Qinghong Zhao
Single nucleotide polymorphisms (SNPs) in the 3'-untranslated regions targeted by putative mircoRNA can change its binding strength, affecting the susceptibility and prognosis of cancer. We aimed to investigate the associations between SNPs within miR-148a binding sites and gastric cancer (GC) risk and prognosis. Using bioinformatics tools, we selected two SNPs (SCRN1 rs6976789 and PDYN rs2235749) located in miR-148a target sites. We genotyped the two SNPs in a case-control study comprising 753 GC patients and 949 cancer-free subjects...
2014: Scientific Reports
Isabell Hennemeier, Hans-Ulrich Humpf, Michael Gekle, Gerald Schwerdt
Ochratoxin A (OTA) is an ubiquitous mycotoxin suspected to cause fibrotic kidney diseases. The involvement of mircoRNAs in these processes is unknown. Here, we investigated the role of the anti-fibrotic miR-29b in OTA-induced alterations of cellular collagen homeostasis. OTA exposure of human embryonic kidney cells (HEK293) cells led to an increase of collagen I, III and IV protein amounts without changes in collagen mRNA expression levels, indicating post-transcriptionally mediated mechanisms potentially involving microRNAs and 3'UTRs of collagen mRNAs...
October 3, 2014: Toxicology
Hanyang Hu, Xing Lu, Xiang Cen, Xiaohua Chen, Feng Li, Shan Zhong
Cadmium is a common toxicant that is detrimental to many tissues. Although a number of transcriptional signatures have been revealed in different tissues after cadmium treatment, the genes involved in the cadmium caused male reproductive toxicity, and the underlying molecular mechanism remains unclear. Here we observed that the mice treated with different amount of cadmium in their rodent chow for six months exhibited reduced serum testosterone. We then performed RNA-seq to comprehensively investigate the mice testicular transcriptome to further elucidate the mechanism...
2014: BioMed Research International
Xiaoping He, Yang Cao, Lihua Wang, Yingli Han, Xiuying Zhong, Guixiang Zhou, Yongping Cai, Huafeng Zhang, Ping Gao
Induction of pluripotent stem cells (iPSC) by defined transcription factors is the recognized canonical means for somatic reprogramming, however, it remains incompletely understood how individual transcription factors affect cell fate decisions during the reprogramming process. Here, we report induction of fibroblast reprogramming by various transcriptional factors is mediated by a miR19a/b-PTEN axis. cMyc, one of the four Yamanaka factors known to stimulate both somatic cell reprogramming and tumorigenesis, induced the expression of multiple mircoRNAs, miR-17 ∼ 92 cluster in particular, in the early stage of reprogramming of human fibroblasts...
2014: PloS One
Saloni Agrawal, Brahim Chaqour
Ischemic retinopathies are clinically well-defined chronic microvascular complications characterized by gradually progressive alterations in the retinal microvasculature and a compensatory aberrant neovascularization of the eye. The subsequent metabolic deficiencies result in structural and functional alterations in the retina which is highly susceptible to injurious stimuli such as diabetes, trauma, hyperoxia, inflammation, aging and dysplipidemia. Emerging evidence indicates that an effective therapy may require targeting multiple components of the angiogenic pathway...
February 26, 2014: World Journal of Biological Chemistry
Ai-qin Liu, Sha-sha Li, Xuan Zhou, Xu-dong Wang, Lun Zhang
OBJECTIVE: To investigate the effect and mechanism of signal transducers and activators of transcription 3 (STAT-3) modulates human tongue squamous cell carcinoma invasion ability via targeting mircoRNA-21. METHODS: Tscca and Tca8113P160 human tongue squamous cell carcinoma cell lines were used.WP1066 (STAT-3 inhibitor) , the small molecule inhibitor of STAT-3 was used to suppress the STAT-3 expression. The half maximal inhibitory concentration (IC50 value) of WP1066 in the two cell lines was determined by methyl thiazolyl tetrazolium (MTT) assay...
September 2013: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
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