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https://www.readbyqxmd.com/read/28735515/determination-of-cell-fate-in-skeletal-muscle-following-bmp-gene-transfer-by-in-vivo-electroporation
#1
Mariko Kawai, Yu-Ki Ohmori, Mai Nishino, Masayo Yoshida, Kaori Tabata, Do-Saku Hirota, Ayako Ryu-Mon, Hiromitsu Yamamoto, Junya Sonobe, Yo-Hei Kataoka, Noriko Shiotsu, Mika Ikegame, Hiroki Maruyama, Toshio Yamamoto, Kazuhisa Bessho, Kiyoshi Ohura
We previously developed a novel method for gene transfer, which combined a non-viral gene expression vector with transcutaneous in vivo electroporation. We applied this method to transfer the bone morphogenetic protein (BMP) gene and induce ectopic bone formation in rat skeletal muscles. At present, it remains unclear which types of cells can differentiate into osteogenic cells after BMP gene transfer by in vivo electroporation. Two types of stem cells in skeletal muscle can differentiate into osteogenic cells: muscle-derived stem cells, and bone marrow-derived stem cells in the blood...
May 5, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28731368/computerized-planning-of-prostate-cryosurgery-and-shape-considerations
#2
Purva Joshi, Anjali Sehrawat, Yoed Rabin
The current study aims to explore possible relationships between various prostate shapes and the difficulty in creating a computer-based plan for cryosurgery. This research effort is a part of an ongoing study to develop computational means in order to improve cryosurgery training and education. This study uses a computerized planner-a key building block of a recently developed prototype for cryosurgery training. The quality of planning is measured by the overall defect volume, a proprietary concept which refers to undercooled areas internal to the target region and overcooled areas external to it...
January 1, 2017: Technology in Cancer Research & Treatment
https://www.readbyqxmd.com/read/28728082/oral-infections-comorbidities-and-sensory-evidences-in-elderly-cross-sectional-study
#3
Nathalia Santos Viana Silva, Luciana Alvarenga da Silva, Omar Jaluul, Wilson Jacob-Filho, Silvia Regina Dowgan Tesseroli de Siqueira
BACKGROUND: Oral infections affect the general health and overlap with chronic diseases due to infectious-immune mechanisms. On the other side, sensory abnormalities may be symptoms of this association. OBJECTIVE: To evaluate the prevalence of oral infections, comorbidities, health parameters and sensory abnormalities in elderly patients. METHODS: Thirty (30) elderly with mean age 70.4 yo, distributed according to ages were evaluated with a protocol that included demographics, comorbidities, medications, laboratory tests, blood pressure, heart rate, mini-mental state examination, clinical oral evaluation and systematized sensory testing (gustative, olfactory, thermal, mechanical and pain thresholds)...
July 1, 2017: Archives of Gerontology and Geriatrics
https://www.readbyqxmd.com/read/28728007/direct-immune-detection-of-cortisol-by-chemiresistor-graphene-oxide-sensor
#4
Yo-Han Kim, Kyungmin Lee, Hunsang Jung, Hee Kyung Kang, Jihoon Jo, In-Kyu Park, Hyun Ho Lee
In this study, a biosensor to detect a stress biomarker of cortisol using cortisol monoclonal antibody (c-Mab) covalently immobilized on reduced graphene oxide (rGO) channel as electrical sensing element was demonstrated. Highly specific immune-recognition between the c-Mab and the cortisol was identified and characterized on a basis of resistance change at the rGO channel based chemiresistor sensor achieving the limit of detection of 10pg/mL (27.6 pM). In addition, cortisol concentrations of real human salivary sample and buffer solution of rat adrenal gland acute slices, which could secret the cortisol induced by adrenocorticotropic hormone (ACTH), were directly measured by the chemiresistor corresponding to the specific sensing of the cortisol...
July 11, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28725968/microglial-mediated-pdgf-cc-activation-increases-cerebrovascular-permeability-during-ischemic-stroke
#5
Enming Joseph Su, Chunzhang Cao, Linda Fredriksson, Ingrid Nilsson, Christina Stefanitsch, Tamara K Stevenson, Juanjuan Zhao, Margret Ragsdale, Yu-Yo Sun, Manuel Yepes, Chia-Yi Kuan, Ulf Eriksson, Dudley K Strickland, Daniel A Lawrence, Li Zhang
Treatment of acute ischemic stroke with the thrombolytic tissue plasminogen activator (tPA) can significantly improve neurological outcomes; however, thrombolytic therapy is associated with an increased risk of intra-cerebral hemorrhage (ICH). Previously, we demonstrated that during stroke tPA acting on the parenchymal side of the neurovascular unit (NVU) can increase blood-brain barrier (BBB) permeability and ICH through activation of latent platelet-derived growth factor-CC (PDGF-CC) and signaling by the PDGF receptor-α (PDGFRα)...
July 19, 2017: Acta Neuropathologica
https://www.readbyqxmd.com/read/28723816/importance-of-physical-qualities-for-speed-and-change-of-direction-ability-in-elite-female-soccer-players
#6
Stacey Emmonds, G Nicholson, C Beggs, B Jones, A Bissas
The purpose of this study was to determine the importance of physical qualities for speed and change of direction (CoD) ability in female soccer players. Data were collected on 10 female soccer players who were part of a professional English Women's Super League team. Player assessments included anthropometric (stature and body mass), body composition (dual-energy X-ray absorptiometry), speed (10m, 30m sprint), CoD ability (505 agility), aerobic (Yo-Yo Intermittent Recovery Test), lower-body strength (bilateral knee extensions) and power (countermovement jump [CMJ], squat jump [SJ], 30cm drop jump [DJ]) measures...
July 17, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28719491/greater-effect-of-east-vs-west-travel-on-jet-lag-sleep-and-team-sport-performance
#7
Peter M Fowler, Wade Knez, Stephen Crowcroft, Amy E Mendham, Joanna Miller, Charlie Sargent, Shona Halson, Rob Duffield
PURPOSE: Determine the recovery timeline of sleep, subjective jet-lag and fatigue, and team-sport physical performance following east and west long-haul travel. METHODS: Ten, physically-trained males underwent testing at 09:00 (AM) and 17:00 (PM) local time on four consecutive days two weeks prior to outbound travel (BASE), and the first four days following 21 h of outbound (WEST) and return (EAST) air travel across eight time-zones between Australia and Qatar. Data collection included performance (countermovement jump [CMJ], 20-m sprint and Yo-Yo Intermittent Recovery level 1 [YYIR1] test) and perceptual (jet-lag, motivation, perceived exertion and physical feeling) measures...
July 17, 2017: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/28717259/a-computerized-tutor-prototype-for-prostate-cryotherapy-key-building-blocks-and-system-evaluation
#8
Yoed Rabin, Kenji Shimada, Purva Joshi, Anjali Sehrawat, Robert Keelan, Dona M Wilfong, James T McCormick
This paper focuses on the evaluation of a prototype for a computer-based tutoring system for prostate cryosurgery, while reviewing its key building blocks and their benchmark performance. The tutoring system lists geometrical constraints of cryoprobe placement, displays a rendered shape of the prostate, simulates cryoprobe insertion, enables distance measurements, simulates the corresponding thermal history, and evaluates the mismatch between the target region shape and a pre-selected planning isotherm. The quality of trainee planning is measured in comparison with a computer-generated plan, created for each case study by a previously developed planning algorithm, known as bubble-packing...
January 28, 2017: Proceedings of SPIE
https://www.readbyqxmd.com/read/28713842/femtosecond-time-resolved-x-ray-absorption-spectroscopy-of-anatase-tio2-nanoparticles-using-xfel
#9
Yuki Obara, Hironori Ito, Terumasa Ito, Naoya Kurahashi, Stephan Thürmer, Hiroki Tanaka, Tetsuo Katayama, Tadashi Togashi, Shigeki Owada, Yo-Ichi Yamamoto, Shutaro Karashima, Junichi Nishitani, Makina Yabashi, Toshinori Suzuki, Kazuhiko Misawa
The charge-carrier dynamics of anatase TiO2 nanoparticles in an aqueous solution were studied by femtosecond time-resolved X-ray absorption spectroscopy using an X-ray free electron laser in combination with a synchronized ultraviolet femtosecond laser (268 nm). Using an arrival time monitor for the X-ray pulses, we obtained a temporal resolution of 170 fs. The transient X-ray absorption spectra revealed an ultrafast Ti K-edge shift and a subsequent growth of a pre-edge structure. The edge shift occurred in ca...
July 2017: Structural Dynamics (Melville, N.Y.)
https://www.readbyqxmd.com/read/28710511/cerebellar-degeneration-related-proteins-2-and-2-like-are-present-in-ovarian-cancer-in-patients-with-and-without-yo-antibodies
#10
Margrethe Raspotnig, Mette Haugen, Maria Thorsteinsdottir, Ingunn Stefansson, Helga B Salvesen, Anette Storstein, Christian A Vedeler
BACKGROUND: Cerebellar degeneration-related protein 2 (CDR2) has been presumed to be the main antigen for the onconeural antibody Yo, which is strongly associated with ovarian cancer and paraneoplastic cerebellar degeneration (PCD). Recent data show that Yo antibodies also target the CDR2-like protein (CDR2L). We, therefore, examined the expression of CDR2 and CDR2L in ovarian cancer tissue from patients with and without Yo antibodies and from various other cancerous and normal human tissues...
July 14, 2017: Cancer Immunology, Immunotherapy: CII
https://www.readbyqxmd.com/read/28707742/intramolecular-locked-dithioalkylbithiophene-based-semiconductors-for-high-performance-organic-field-effect-transistors
#11
Sureshraju Vegiraju, Bo-Chin Chang, Pragya Priyanka, Deng-Yi Huang, Kuan-Yi Wu, Long-Huan Li, Wei-Chieh Chang, Yi-Yo Lai, Shao-Huan Hong, Bo-Chun Yu, Chien-Lung Wang, Wen-Jung Chang, Cheng-Liang Liu, Ming-Chou Chen, Antonio Facchetti
New 3,3'-dithioalkyl-2,2'-bithiophene (SBT)-based small molecular and polymeric semiconductors are synthesized by end-capping or copolymerization with dithienothiophen-2-yl units. Single-crystal, molecular orbital computations, and optical/electrochemical data indicate that the SBT core is completely planar, likely via S(alkyl)⋯S(thiophene) intramolecular locks. Therefore, compared to semiconductors based on the conventional 3,3'-dialkyl-2,2'-bithiophene, the resulting SBT systems are planar (torsional angle <1°) and highly π-conjugated...
July 14, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28705217/monitoring-clinical-and-microbiological-evolution-of-a-cystic-fibrosis-patient-over-26%C3%A2-years-experience-of-a-brazilian-cf-centre
#12
Cassiana da Costa Ferreira Leite, Tania Wrobel Folescu, Mônica de Cássia Firmida, Renata Wrobel Folescu Cohen, Robson Souza Leão, Flávia Alvim Dutra de Freitas, Rodolpho Mattos Albano, Claudia Henrique da Costa, Elizabeth Andrade Marques
BACKGROUND: Burkholderia cepacia complex is a group of opportunistic pathogens in cystic fibrosis (CF) patients believed to be associated with poor prognosis and patient-to-patient transmissibility. Little is known about clinical outcomes after B. vietnamiensis chronic colonization/infection. CASE PRESENTATION: A 33 yo male patient had diagnosis of CF by 7 yo, after recurrent pneumonia during infancy and lobectomy (left upper lobe) at 6 yo. Burkholderia cepacia complex (Bcc) was first isolated by 13 yo, and the patient fulfilled the criteria for chronic colonization by 15 yo...
July 14, 2017: BMC Pulmonary Medicine
https://www.readbyqxmd.com/read/28698412/-cardiac-rehabilitation-in-minimally-invasive-cardiac-surgery-coronary-artery-bypass-grafting
#13
Yo Takahashi, Akihiko Yamauchi, Masaki Tabuchi, Daisuke Toritsuka, Osamu Arasaki, Masahiro Tamashiro, Masanori Kakazu, Katsuaki Iju, Mitsuru Kume, Shogo Sakihama, Yuta Matayoshi
OBJECTIVES: A purpose is to compare the in-hospital rehabilitation progress after minimally invasive cardiac surgery coronary artery bypass grafting (MICS CABG) with that after off pump coronary artery bypass grafting( OPCAB). METHODS: We analyzed 60 patients who underwent coronary artery bypass grafting surgery from January 2015 to December 2016. Fourteen patients underwent MICS CABG (MICS CABG group) and 46 patients underwent OPCAB (OPCAB group). The post-operative days to start standing position and independent walking, and hospitalization duration were analyzed between the 2 groups...
July 2017: Kyobu Geka. the Japanese Journal of Thoracic Surgery
https://www.readbyqxmd.com/read/28696046/survival-impacts-of-lymphocyte-infiltration-into-the-tumor-of-hepatocellular-carcinoma-in-hbv-positive-or-non-b-non-c-patients-who-underwent-curative-resection
#14
Shigeki Nakagawa, Naoki Umezaki, Takanobu Yamao, Takayoshi Kaida, Hirohisa Okabe, Kosuke Mima, Katsunori Imai, Daisuke Hashimoto, Yo-Ichi Yamashita, Takatoshi Ishiko, Akira Chikamoto, Hideo Baba
AIM: The prognostic value of lymphocyte infiltration into hepatocellular carcinoma (HCC) is still controversial, and it has not been reported in HBV- and non-B non-C HCC. The aim of this study is to assess the prognostic significance of lymphocyte infiltrate in tumor for the patients of HBV positive and non-B non-C (NBNC) HCC patients. METHODS: This study investigated 145 HBV positive or NBNC patients who underwent hepatectomy for HCC between January 2001 and May 2009...
July 11, 2017: Hepatology Research: the Official Journal of the Japan Society of Hepatology
https://www.readbyqxmd.com/read/28691180/examining-the-association-between-language-expository-discourse-and-offending-behaviour-an-investigation-of-direction-strength-and-independence
#15
Thomas Hopkins, Judy Clegg, Joy Stackhouse
BACKGROUND: A high prevalence of Developmental Language Disorder (DLD) is reported in the population of Young Offenders (YO). However, little is known about the extent of the association between language and offending behaviour relative to social disadvantage, education attendance and non-verbal intelligence (IQ), and neither has this association been investigated with particular reference to the expository discourse abilities of YOs on community orders in the UK. AIMS: This study aimed to examine the direction and strength of the association between language and offending behaviour by comparing the receptive and expressive language and expository discourse abilities of male and female YOs and non-offenders in the UK, relative to the confounds of social disadvantage, years of education attended and non-verbal IQ...
July 10, 2017: International Journal of Language & Communication Disorders
https://www.readbyqxmd.com/read/28684854/prospectively-isolated-mesenchymal-stem-stromal-cells-are-enriched-in-the-cd73-population-and-exhibit-efficacy-after-transplantation
#16
Eriko Grace Suto, Yo Mabuchi, Nobuharu Suzuki, Koji Suzuki, Yusuke Ogata, Miyu Taguchi, Takeshi Muneta, Ichiro Sekiya, Chihiro Akazawa
Mesenchymal stem/stromal cells (MSCs), which reside in the bone marrow (BM) and various other tissues, can self-renew and differentiate into mesenchymal lineages. Many groups have harvested rat MSCs (rMSCs) from rat BM (rBM) by using a flush-out procedure and have evaluated surface marker expression after long-term culture. However, MSCs gradually differentiate during expansion and exhibit altered proliferation rates, morphological features and functions in vitro. Variations in MSC isolation methods may alter the effectiveness of therapeutic applications...
July 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28681419/linear-and-nonlinear-relationships-between-body-mass-index-and-physical-fitness-in-brazilian-children-and-adolescents
#17
Vítor P Lopes, Marco Cossio-Bolaños, Rossana Gómez-Campos, Miguel de Arruda, Jefferson Eduardo Hespanhol, Luis P Rodrigues
OBJECTIVES: The purpose of this study was to evaluate the linear and curvilinear relationship between body mass index (BMI) and physical fitness in children and adolescents. METHODS: Participants were 4567 (2505 girls) children aged 6-16 years. Weight and height were measured and BMI was calculated and adjusted for age and sex using WHO z-scores. Physical fitness was evaluated with the following tests: Yo-Yo test, standing long jump, seated 2-kg medicine ball throw, and 20-m dash...
July 5, 2017: American Journal of Human Biology: the Official Journal of the Human Biology Council
https://www.readbyqxmd.com/read/28679599/self-reported-sleep-and-alzheimer-disease-csf-biomarkers-a-wake-up-call
#18
EDITORIAL
Adam P Spira, Yo-El S Ju
No abstract text is available yet for this article.
July 5, 2017: Neurology
https://www.readbyqxmd.com/read/28677942/effect-of-fibular-repositioning-taping-in-adult-basketball-players-with-chronic-ankle-instability-a-randomized-placebo-controlled-crossover-trial
#19
Yanina Alves, Fernando Ribeiro, Anabela G Silva
BACKGROUND: Chronic ankle instability presents a high incidence and prevalence in basketbal players. It's important to develop strategies to reduce the functional and mechanical limitations resulting from this condition. To compare the effect of Mulligan ́s fibular repositioning taping with a placebo taping immediatly after application and after a running test (Yo-Yo IRT). METHODS: 16 adult basketball players (10 male, 6 female) with chronic ankle instability and mean age 21...
July 5, 2017: Journal of Sports Medicine and Physical Fitness
https://www.readbyqxmd.com/read/28675368/ante-situm-liver-resection-for-giant-hepatic-tumour-case-report-and-review-of-literature
#20
Vladislav Braşoveanu, Mihail Pautov, Mihnea Ioan Ionescu, Claudiu Anghel, Dan Ionut Dudus, Manish Moothoor, Florin Ichim, Eliza Gangone, Ion Barbu
Ex-situ liver surgery refers to complex liver resections involving hepatic vascular exclusion and a warm ischemia time (WIT) of more than 90 minutes that allows liver resection and vascular reconstruction in patients with giant liver tumours with a difficult approach . Ante-situm liver resections, otherwise called "œex-situ in-vivo" resections is achieved through externalization of the liver outside of the abdominal cavity by clamping and sectioning of the efferent pedicles (suprahepatic veins) ("ex situ") without cutting the afferent vascular pedicle ("in vivo"), thus leaving the hepatic pedicle intact...
May 2017: Chirurgia
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