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Flourescence imaging in tumor mice

Jie Ding, Min Feng, Wenxian Zhi, Wenxian Guan
OBJECTIVE: To explore the tracing role of nanometer microspheres tagged by fluorescent dye NIR-797 in gastric cancer cells in order to guide precise resection for gastric cancer. METHODS: Targeted polymer segment NH2-PEG-Pep-PCL and non-targeted polymer segment NH2-PEG- PCL(without Pep) were synthesized with NH2-PEG-NH2, gelatinase substrate peptides (Pep) and PCL-COOH by amidation method. Then nanometer microspheres were prepared by self-assemebly method. NIR-797 was linked into the end of PEG through chemical reaction...
February 2015: Zhonghua Wei Chang Wai Ke za Zhi, Chinese Journal of Gastrointestinal Surgery
Philip C Gach, Peter J Attayek, Gabriela Herrera, Jen Jen Yeh, Nancy L Allbritton
Described is the construction of a large array of releasable microstructures (micropallets) along with screening and isolation protocols for sorting rare, approximately 1 in 10,000, cancer stem cells (CSCs) from a heterogeneous cell population. A 10.1 × 7.1 cm array of micropallets (50 × 50 × 75 μm structures and 25 μm micropallet gap) was fabricated on a large glass substrate, providing an array of approximately 1.3 million releasable microstructures. Image analysis algorithms were developed to permit array screening for identification of fluorescently labeled cells in less than 15 min using an epifluorescent wide-field microscope with a computer controlled translational stage...
August 6, 2013: Analytical Chemistry
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