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https://www.readbyqxmd.com/read/28092777/controlling-the-3d-architecture-of-self-lifting-auto-generated-tissue-equivalents-slates-for-optimized-corneal-graft-composition-and-stability
#1
Ricardo M Gouveia, Elena González-Andrades, Juan C Cardona, Carmen González-Gallardo, Ana M Ionescu, Ingrid Garzon, Miguel Alaminos, Miguel González-Andrades, Che J Connon
Ideally, biomaterials designed to play specific physical and physiological roles in vivo should comprise components and microarchitectures analogous to those of the native tissues they intend to replace. For that, implantable biomaterials need to be carefully designed to have the correct structural and compositional properties, which consequently impart their bio-function. In this study, we showed that the control of such properties can be defined from the bottom-up, using smart surface templates to modulate the structure, composition, and bio-mechanics of human transplantable tissues...
December 23, 2016: Biomaterials
https://www.readbyqxmd.com/read/28092608/white-and-red-blood-cell-picture-in-rabbits-experimentally-infected-with-strains-of-the-rabbit-haemorrhagic-disease-rhd-virus-without-or-with-variable-haemagglutination-capacity
#2
P Niedźwiedzka-Rystwej, B Tokarz-Deptuła, W Deptuła
The aim of the study was to establish if haemagglutination of rabbit haemorrhagic disease virus (RHDV) affects haematological picture of peripheral blood in rabbits and the pathogenicity of the virus. The study analyzed white and red blood cell picture in rabbits experimentally infected with two non-haemagglutinating (HA-) RHDV strains (Frankfurt and Asturias) and one strain with variable haemagglutination capacity (HA+/-) (Hagenow). Studies with HA- and HA +/- are rare and relate only to 4 HA- strains (2 RHDV: BLA and Rainham; 2 RHDVa: Pv97 and 9905) and 1 HA+/- RHDV strain: ŻD, where less changes in haematological indices and less pathogenicity were observed...
December 1, 2016: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28091998/palliative-effects-of-extra-virgin-olive-oil-gallic-acid-and-lemongrass-oil-dietary-supplementation-on-growth-performance-digestibility-carcass-traits-and-antioxidant-status-of-heat-stressed-growing-new-zealand-white-rabbits
#3
Adham A Al-Sagheer, Ahmed H Daader, Hassan A Gabr, Elham A Abd El-Moniem
This study explored the effects of supplemental dietary extra virgin olive oil (EVOO), gallic acid (GA), or lemongrass essential oil (LGEO) on growth performance, nutrient digestibility, carcass traits, lipid peroxidation, hematological, and antioxidative status in growing rabbits under heat stress conditions. A total of 48 male growing New Zealand White rabbits were randomly divided into four equal groups, which received a basal diet without any supplementation or supplemented with 15 g EVOO, 500 mg GA, or 400 mg LGEO/kg of diet, for eight consecutive weeks...
January 14, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28091958/identification-and-anticoagulant-activity-of-a-novel-kunitz-type-protein-ha11-from-the-salivary-gland-of-the-tick-hyalomma-asiaticum
#4
Houshuang Zhang, Ruiqiong Qiao, Haiyan Gong, Jie Cao, Yongzhi Zhou, Jinlin Zhou
Kunitz/bovine pancreatic trypsin inhibitor proteins are abundant in the salivary glands of ticks and perform multiple functions in blood feeding, including inhibiting blood coagulation, regulating host blood supply and disrupting host angiogenesis. In this study, we identified a novel gene designated HA11 (Hyalomma asiaticum 11 kDa protein) from the salivary gland of the tick H. asiaticum. HA11 is encoded by a gene with an open reading frame of 306 bp that is translated into a deduced 101 amino acid 11 kDa protein that shares 27% sequence identity with a Kunitz-like protease inhibitor precursor in Amblyomma variegatum...
January 13, 2017: Experimental & Applied Acarology
https://www.readbyqxmd.com/read/28091908/effect-of-tramadol-on-rabbit-uterine-contractile-activity-induced-in-late-pregnancy
#5
A A Yakovleva, L A Nazarova, V M Prokopenko, N G Pavlova
Effect of Tramadol infusion (5 mg/ml) on oxytocin-induced uterine contractile activity was studied in chronic experiment on female rabbits with different degrees of biological readiness for parturition. In case of sufficient biological readiness for parturition, Tramadol did not change the number of uterine contractions, but increased the amplitude and duration of each contraction against the background of increased creatine phosphate consumption by the myometrium. At the same time, Tramadol infusion to females without biological readiness for partirition suppressed induced uterine contractile activity by reducing the amplitude of each uterine contraction...
January 14, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28091810/severe-lead-toxicosis-in-a-lionhead-rabbit
#6
Kristen M Walter, Karyn Bischoff, Ricardo de Matos
INTRODUCTION: Lead toxicosis occurs in veterinary patients, with few reports involving rabbits, and no previous reports using oral calcium disodium EDTA. CASE REPORT: A 7-year-old male castrated Lionhead rabbit presented to the Cornell University Hospital for Animals (CUHA) for evaluation after a 2-day history of lethargy and a 2-week history of hyporexia. The patient had been observed pulling paint from the walls of the home, a house built circa 1900, in the months prior to presentation...
January 13, 2017: Journal of Medical Toxicology: Official Journal of the American College of Medical Toxicology
https://www.readbyqxmd.com/read/28091772/transient-expression-of-recombinant-tissue-plasminogen-activator-rt-pa-gene-in-cucurbit-plants-using-viral-vector
#7
Vahid Jalali Javaran, Alireza Shafeinia, Mokhtar Jalali Javaran, Esmaeil Ghasemi Gojani, Malihe Mirzaee
OBJECTIVE: To use a transient expression system to express a truncated human tissue plasminogen activator (K2S) gene in cucurbit plants. RESULTS: The recombinant tissue plasminogen activator protein (K2S form) was expressed in active form in cucurbit plants. Its molecular weight was 43 kDa. The plant-derived rt-PA was determined using goat anti-rabbit antibody by western blotting. Among the infected lines, the highest expression of rt-PA was 62 ng/100 mg per leaf tissue as measured by ELISA...
January 16, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28091680/protective-effects-of-dietary-glycine-and-glutamic-acid-toward-the-toxic-effects-of-oxidized-mustard-oil-in-rabbits
#8
Alam Zeb, Saleem Ur Rahman
The protective role of glycine and glutamic acid against the toxic effects of oxidized oil was studied for the first time. Mustard seed oil was thermally oxidized and characterized for quality characteristics and polyphenolic composition using reversed phase HPLC-DAD. Significant changes in the quality characteristics occurred with thermal oxidation. Fourteen polyphenolic compounds were identified and quantified in oils. Quercetin-3-glucoside, quercetin-3-feruloylsophoroside, catechin, quercetin-3-rutinoside, quercetin-3,7-diglucoside, sinapic acid and vanillic acid hexoside were the major compounds in the fresh and oxidized oil...
January 16, 2017: Food & Function
https://www.readbyqxmd.com/read/28091604/polyglucose-nanoparticles-with-renal-elimination-and-macrophage-avidity-facilitate-pet-imaging-in-ischaemic-heart-disease
#9
Edmund J Keliher, Yu-Xiang Ye, Gregory R Wojtkiewicz, Aaron D Aguirre, Benoit Tricot, Max L Senders, Hannah Groenen, Francois Fay, Carlos Perez-Medina, Claudia Calcagno, Giuseppe Carlucci, Thomas Reiner, Yuan Sun, Gabriel Courties, Yoshiko Iwamoto, Hye-Yeong Kim, Cuihua Wang, John W Chen, Filip K Swirski, Hsiao-Ying Wey, Jacob Hooker, Zahi A Fayad, Willem J M Mulder, Ralph Weissleder, Matthias Nahrendorf
Tissue macrophage numbers vary during health versus disease. Abundant inflammatory macrophages destruct tissues, leading to atherosclerosis, myocardial infarction and heart failure. Emerging therapeutic options create interest in monitoring macrophages in patients. Here we describe positron emission tomography (PET) imaging with (18)F-Macroflor, a modified polyglucose nanoparticle with high avidity for macrophages. Due to its small size, Macroflor is excreted renally, a prerequisite for imaging with the isotope flourine-18...
January 16, 2017: Nature Communications
https://www.readbyqxmd.com/read/28091410/molecular-mechanisms-of-increased-heart-rate-in-shenxianshengmai-treated-bradycardia-rabbits
#10
Zhou-Ying Liu, Jian Huang, Na-Na Liu, Min Zheng, Tao Zhao, Bu-Chang Zhao, Yi-Min Wang, Jie-Lin Pu
BACKGROUND: The molecular mechanisms of Shenxianshengmai (SXSM), a traditional Chinese medicine, on bradycardia have been incompletely understood. The study tried to investigate the gene expression profile and proteomics of bradycardia rabbits' hearts after SXSM treatment. METHODS: Twenty-four adult rabbits were randomly assigned in four groups: sham, model, model plus SXSM treatment, and sham plus SXSM treatment groups. Heart rate was recorded in all rabbits. Then, total RNA of atria and proteins of ventricle were isolated and quantified, respectively...
2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28089826/comparison-of-histologic-healing-and-biomechanical-characteristics-between-repair-techniques-for-a-delaminated-rotator-cuff-tear-in-rabbits
#11
Sang-Jin Cheon, Jung-Han Kim, Heui-Chul Gwak, Chang-Wan Kim, Jeon-Kyo Kim, Ji-Hwan Park
BACKGROUND: The purpose of this study was to compare histologic healing and biomechanical characteristics between 2 repair techniques (layer by layer, repair of each layer to bone separately; and whole layer, repair of each layer to the bone en masse) for delaminated rotator cuff tear. MATERIALS AND METHODS: Rabbits were used as subjects and classified into 2 groups: group A, right side, the layer-by-layer repair group; and group B, left side, the whole-layer repair group...
January 12, 2017: Journal of Shoulder and Elbow Surgery
https://www.readbyqxmd.com/read/28089797/distal-femoral-hemiepiphysiodesis-using-screw-and-non-absorbable-filament-for-the-treatment-of-idiopatic-genu-valgum-preliminary-results-of-12-knees
#12
Gino Martínez Soto, Sebastián Drago Pérez, Carolina Avilés Espinoza, Angélica Ibañez León, Felipe Hodgson Ovalle, Constanza Ramírez Mac-Lean
INTRODUCTION: Different techniques for gradual correction of angular deformities of lower limbs exist. Screws and nonabsorbable filament has been described as an effective alternative for transitory hemiepiphysiodesis in New Zealand rabbits. HYPOTHESIS: Hemiepiphysiodesis using screws and non-absorbable filament is effective in pediatric population, for correction of genu valgum. METHODS: Retrospective evaluation, 12 knees in 6 patients younger than 15 years (3 female), operated because of genu valgum...
January 9, 2017: Orthopaedics & Traumatology, Surgery & Research: OTSR
https://www.readbyqxmd.com/read/28089755/biodistribution-and-retention-of-locally-administered-human-mesenchymal-stromal-cells-quantitative-polymerase-chain-reaction-based-detection-of-human-dna-in-murine-organs
#13
Michael Creane, Linda Howard, Timothy O'Brien, Cynthia M Coleman
BACKGROUND: Determining the distributive fate and retention of a cell therapy product after administration is an essential part of characterizing it's biosafety profile. Therefore, regulatory guidelines stipulate that biodistribution assays are a requirement prior to advancing a cell therapy to the clinic. Here the development of a highly sensitive quantitative polymerase chain reaction (qPCR)-based method of tracking the biodistribution and retention of human mesenchymal stromal cells (hMSCs) in mice, rats or rabbits is described...
January 12, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28089661/studying-the-influence-of-formulation-and-process-variables-on-vancomycin-loaded-polymeric-nanoparticles-as-potential-carrier-for-enhanced-ophthalmic-delivery
#14
Carol Yousry, Seham A Elkheshen, Hanan M El-Laithy, Tamer Essam, Rania H Fahmy
Ocular topically applied Vancomycin (VCM) suffers poor bioavailability due to its high molecular weight and hydrophilicity. In the present investigation, VCM-loaded polymeric nanoparticles (PNPs) were developed aiming to enhance its ocular bioavailability through prolonging its release pattern and ophthalmic residence. PNPs were prepared utilizing double emulsion (W/O/O), solvent evaporation technique. 2(3)×4(1) full factorial design was applied to evaluate individual and combined influences of polymer type, Eudragit® RS100, sonication time, and Span®80 concentration on PNPs particle size, encapsulation efficiency, and zeta potential...
January 12, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28089032/rule-of-three-for-esophageal-dilation-like-the-tortoise-versus-the-rabbit-low-and-slow-is-our-friend-and-our-patients-win
#15
EDITORIAL
Joel E Richter
No abstract text is available yet for this article.
February 2017: Gastrointestinal Endoscopy
https://www.readbyqxmd.com/read/28088634/chitosan-g-poly-n-isopropylacrylamide-copolymers-as-delivery-carriers-for-intracameral-pilocarpine-administration
#16
Jui-Yang Lai, Li-Jyuan Luo
This study reports, for the first time, the development of a chitosan-g-poly(N-isopropylacrylamide) (Chi-PN) biodegradable in situ gelling delivery system for ocular pilocarpine administration through intracameral injection. The number of thermo-responsive polymer segments grafted onto the chitosan via carbodiimide-mediated formation of amide linkages was greatly affected by varying the feeding amount of carboxyl-terminated poly(N-isopropylacrylamide) in the synthesis, thereby determining the phase transition temperature and enzymatic degradability of Chi-PN materials...
January 11, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28088341/evaluation-of-an-in-house-indirect-elisa-for-detection-of-antibody-against-haemorrhagic-septicemia-in-asian-elephants
#17
Pallop Tankaew, Thawatchai Singh-La, Chatchote Titaram, Veerasak Punyapornwittaya, Preeyanat Vongchan, Takuo Sawada, Nattawooti Sthitmatee
Pasteurella multocida causes haemorrhagic septicemia in livestock and wild animals, including elephants. The disease has been reported in Asian elephants in India and Sri Lanka, but to date there have been no reported cases in Thailand. ELISA or indirect hemagglutination assays (IHA) have been demonstrated to be able to detect the antibody against the disease in cattle, but no data are available for elephants. The present study reports a novel in-house indirect ELISA for antibody detection of haemorrhagic septicemia in Asian elephants, and evaluates the sensitivity and specificity of the method using a Bayesian approach...
January 11, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28088280/comparative-study-of-colloidal-gold-and-quantum-dots-as-labels-for-multiplex-screening-tests-for-multi-mycotoxin-detection
#18
Astrid Foubert, Natalia V Beloglazova, Sarah De Saeger
Quantum dots (QDs) and colloidal gold nanoparticles (CG) were evaluated as labels for multiplex lateral flow immunoassay (LFIA) for determination of mycotoxins deoxynivalenol (DON), zearalenone (ZEN) and T2/HT2-toxin (T2/HT2) in cereal matrices. Both developed assays were based on the same immunoreagents (except for the labels), therefore their analytical characteristics could be objectively compared. For both LFIAs antigens (DON-ovalbumin (OVA), ZEN-OVA and T2-OVA) and rabbit anti-mouse immunoglobulin were immobilized on a nitrocellulose membrane as three test lines and one control line, respectively...
February 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28088071/discrete-quasi-linear-viscoelastic-damping-analysis-of-connective-tissues-and-the-biomechanics-of-stretching
#19
Behzad Babaei, Aaron J Velasquez-Mao, Stavros Thomopoulos, Elliot L Elson, Steven D Abramowitch, Guy M Genin
The time- and frequency-dependent properties of connective tissue define their physiological function, but are notoriously difficult to characterize. Well-established tools such as linear viscoelasticity and the Fung quasi-linear viscoelastic (QLV) model impose forms on responses that can mask true tissue behavior. Here, we applied a more general discrete quasi-linear viscoelastic (DQLV) model to identify the static and dynamic time- and frequency-dependent behavior of rabbit medial collateral ligaments. Unlike the Fung QLV approach, the DQLV approach revealed that energy dissipation is elevated at a loading period of ∼10s...
December 22, 2016: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28081478/pulmonary-sarcomatoid-carcinoma-with-alk-rearrangement-frequency-clinical-pathologic-characteristics-and-response-to-alk-inhibitor
#20
Xinru Chen, Yu Zhang, Jiabin Lu, Chunwei Xu, Jianzhong Liang, Fang Wang, Wenyong Sun, Sangao Fang, Jingping Yuan, Huijuan Wang, Hui Wang, Xuewen Liu, Likun Chen
PURPOSE: The incidence of anaplastic lymphoma kinase (ALK) rearrangement in pulmonary sarcomatoid carcinoma (PSC) is controversial. In this study, we aimed to reveal the reliable frequency and the clinical-pathologic characteristics of pulmonary sarcomatoid carcinoma (PSC) with ALK rearrangement in Chinese population, and to provide insight into the translatability of anti-ALK treatment in this treatment-refractory disease. METHODS: Immunohistochemistry (IHC) using a Ventana anti-ALK (D5F3) rabbit monoclonal antibody was performed in 141 PSC specimens collected from multiple medical centers...
January 9, 2017: Translational Oncology
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