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https://www.readbyqxmd.com/read/28738525/mesenchymal-stem-cells-with-igf-1-and-tgf-%C3%AE-1-in-laminin-gel-for-osteochondral-defects-in-rabbits
#1
Mudasir Bashir Gugjoo, Amarpal, Ahmed Abdelbaset-Ismail, Hari Prasad Aithal, Prakash Kinjavdekar, Abhijit Motiram Pawde, Gutulla Sai Kumar, Gutulla Taru Sharma
OBJECTIVE: Healing of articular cartilage is still a challenge due to its limited potential to regenerate. In the present study, we evaluated allogenic bone marrow mesenchymal stem cells (BM-MSCs) alone or in combination with growth factors, insulin-like growth factor-1 (IGF-1) and transforming growth factor-β1 (TGF-β1) in laminin scaffolds for healing of osteochondral defects. DESIGN: Osteochondral defects of 4mm (diameter) x 5mm (depth) were induced in the rabbit knee joints and treated with phosphate-buffered saline (PBS; control), BM-MSCs, BM-MSCs in laminin, BM-MSCs in laminin with IGF-1, or BM-MSCs in laminin with IGF-1 and TGF-β1 in 10 animals each...
July 19, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28738415/monocarboxylate-transporters-mediate-fluorescein-uptake-in-corneal-epithelial-cells
#2
Yi-Chen Sun, Hau-Min Liou, Po-Ting Yeh, Wei-Li Chen, Fung-Rong Hu
Purpose: To determine the presence of monocarboxylate transporter (MCT) in human and rabbit corneal epithelium and its role in transcellular fluorescein transportation in the cornea. Methods: The presence of MCTs in human and rabbit corneal epithelium was determined by RT-PCR and immunohistochemistry. Intracellular fluorescein uptake experiment was performed using cultured human corneal epithelial cells (HCECs). The involvement of MCT in fluorescein uptake was determined by addition of MCT inhibitors to HCECs and acute dry eye model on New Zealand albino rabbits by spectrophotometry, corneal impression cytology, and external eye photographs...
July 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28738014/enhancer-of-zeste-homologue-2-ezh2-expression-in-synovial-sarcomas-as-a-promising-indicator-of-prognosis
#3
Ulviye Yalçınkaya, Nesrin Uğraş, Gonca Özgün, Gökhan Ocakoğlu, Adem Deligönül, Sibel Kahraman Çetintaş, Muhammed Sadık Bilgen
Synovial sarcoma (SS) is a type of soft tissue sarcoma, often linked to poor survival. Although overexpression of enhancer of zeste homologue 2 (EZH2) has been associated with poor prognosis in different tumors, a few studies investigated this link in SS. Here, we analyzed the relationship between EZH2 expression and prognostic factors in SS. We included 29 patients with SS. Immunostaining of EZH2 was performed with (D2C9) XPTM Rabbit mAb antibody, and the results were classified as low EZH2 expression (negative or weak expression) and high EZH2 expression category (moderate or strong expression)...
July 24, 2017: Bosnian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28738004/bone-marrow-cell-composition-and-morphology-in-healthy-juvenile-female-new-zealand-white-rabbits-oryctolagus-cuniculus
#4
Rebekah M Riedel, Ricardo de Matos, Deanna M W Schaefer
OBJECTIVE To provide contemporary preliminary guidelines for the morphological evaluation of bone marrow in conjunction with CBC results for healthy juvenile (3- to 6-month-old) female New Zealand White rabbits (Oryctolagus cuniculus). ANIMALS 22 female New Zealand White rabbits. PROCEDURES Each rabbit was sedated, and a blood sample (3 mL) was collected from an ear artery for a CBC, after which the rabbit was euthanized. Within 5 minutes after euthanasia, bone marrow samples were obtained from the femur for cytologic and histologic evaluation...
August 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28737980/nitration-of-h2b-histone-elicits-an-immune-response-in-experimental-animals
#5
Md Asad Khan, Khursheed Alam, Syed Mehdi Hassan, M Moshahid A Rizvi
Histone H2B is an autoantigen that appears in circulation due to altered apoptosis/or insufficient clearance and is likely to be involved in the induction and progression of autoimmune diseases since modified-H2B is immunogenic. Our studies demonstrate that tyrosines of H2B histone spontaneously converts to free and nitrotyrosine bound protein in vivo. Commercially available H2B histone was modified with peroxynitrite in vitro. Modified H2B was found to be more immunogenic than native form in experimental animals...
July 24, 2017: Autoimmunity
https://www.readbyqxmd.com/read/28737447/pmab-52-specific-and-sensitive-monoclonal-antibody-against-cat-podoplanin-for-immunohistochemistry
#6
Shinji Yamada, Shunsuke Itai, Takuro Nakamura, Miyuki Yanaka, Noriko Saidoh, Yao-Wen Chang, Saori Handa, Hiroyuki Harada, Yumiko Kagawa, Osamu Ichii, Satoru Konnai, Mika K Kaneko, Yukinari Kato
Podoplanin (PDPN) is expressed in several normal tissues, such as lymphatic endothelial cells, podocytes of renal glomerulus, and type I alveolar cells of lung. PDPN activates platelet aggregation by binding to C-type lectin-like receptor-2 (CLEC-2) on platelet. Although monoclonal antibodies (mAbs) against human PDPN, mouse PDPN, rat PDPN, rabbit PDPN, dog PDPN, and bovine PDPN have been established, anticat PDPN (cPDPN) mAbs have not been developed. In this study, we immunized mice with Chinese hamster ovary (CHO)-K1 cell lines expressing cPDPN, and developed anti-cPDPN mAbs...
July 24, 2017: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/28735730/impressic-acid-from-acanthopanax-koreanum-possesses-matrix-metalloproteinase-13-down-regulating-capacity-and-protects-cartilage-destruction
#7
Hyun Lim, Dong Suk Min, Han Eul Yun, Kil Tae Kim, Ya Nan Sun, Le Duc Dat, Young Ho Kim, Hyun Pyo Kim
ETHNOPHARMACOLOGICAL RELEVANCE: Acanthopanax koreanum (Araliaceae) has been used in traditional medicine for enhancing vitality, rheumatism, and bone-related pains. But its activity on cartilage protection has not been known yet. AIM OF THE STUDY: Matrix metalloproteinase (MMP)-13 has an important role in degrading cartilage materials under pathologic conditions such as arthritis. The present study was designed to find the inhibitory activity of impressic acid on MMP-13 expression and cartilage protective action...
July 20, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28735526/the-biocompatibility-of-bone-cements-progress-in-methodological-approach
#8
Carlo Dall'Oca, Tommaso Maluta, Gian Mario Micheloni, Matteo Cengarle, Giampaolo Morbioli, Paolo Bernardi, Andrea Sbarbati, Daniele Degl'Innocenti, Franco Lavini, Bruno Magnan
The ideal bone graft substitute should have certain properties and there are many studies dealing with mixture of polymethylmetacrilate (PMMA) and β-tricalciumphospate (β-TCP) presenting the best characteristics of both. Scanning Electron Microscopy (SEM), for ultra-structural data, resulted a very reliable in vivo model to better understand the bioactivity of a cement and to properly evaluate its suitability for a particular purpose. The present study aims to further improve the knowledge on osteointegration development, using both parameters obtained with the Environmental Scanning Electron Microscopy (ESEM) and focused histological examination...
May 4, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28734361/preparation-of-intact-mitochondria-using-free-flow-isoelectric-focusing-with-post-ph-gradient-sample-injection-for-morphological-functional-and-proteomics-studies
#9
Yu-Chen He, Fan-Zhi Kong, Liu-Yin Fan, Jane Y Wu, Xiao-Ping Liu, Jun Li, Yan Sun, Qiang Zhang, Ying Yang, Xue-Jing Wu, Hua Xiao, Cheng-Xi Cao
Mitochondria play essential roles in both energy metabolism and cell signaling, which are critical for cell survival. Although significant efforts have been invested in understanding mitochondrial biology, methods for intact mitochondria preparation are technically challenging and remain to be improved. New methods for heterogeneous mitochondria purification will therefore boost our understanding on their physiological and biophysical properties. Herein, we developed a novel recycling free-flow isoelectric focusing (RFFIEF) with post-pH gradient sample injection (post-PGSI) for preparative separation of mitochondria...
August 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28733893/effects-of-new-generation-tmem16a-inhibitors-on-calcium-activated-chloride-currents-in-rabbit-urethral-interstitial-cells-of-cajal
#10
Stephen Fedigan, Eamonn Bradley, Timothy Webb, Roddy J Large, Mark A Hollywood, Keith D Thornbury, Noel G McHale, Gerard P Sergeant
Interstitial cells of Cajal (ICC) isolated from the rabbit urethra exhibit Ca(2+)-activated Cl(-) currents (I ClCa) that are important for the development of urethral tone. Here, we examined if TMEM16A (ANO1) contributed to this activity by examining the effect of "new-generation" TMEM16A inhibitors, CACCinh-A01 and T16Ainh-A01, on I ClCa recorded from freshly isolated rabbit urethral ICC (RUICC) and on contractions of intact strips of rabbit urethra smooth muscle. Real-time quantitative PCR experiments demonstrated that TMEM16A was highly expressed in rabbit urethra smooth muscle, in comparison to TMEM16B and TMEM16F...
July 21, 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/28733828/thermosensitive-in-situ-gel-based-on-solid-dispersion-for-rectal-delivery-of-ibuprofen
#11
Yangdan Liu, Xin Wang, Youping Liu, Xin Di
The objective of this study was to develop a thermosensitive in situ gel based on solid dispersions (SDs) for rectal delivery of ibuprofen (IBU). Thermosensitive (poloxamer 407) and mucoadhesive (hydroxypropylmethyl cellulose E5 and sodium alginate) polymers were used to prepare the in situ gel and the sol-gel transition temperature (T sol-gel) and gel strength were optimized. The in vitro release performance and in vivo pharmacokinetic properties of the in situ gel after their rectal administration to rabbits were investigated...
July 21, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28733649/an-in-vitro-model-for-the-ocular-surface-and-tear-film-system
#12
Qiaozhi Lu, Hongbo Yin, Michael P Grant, Jennifer H Elisseeff
Dry eye is a complicated ocular surface disease whose exact pathogenesis is not yet fully understood. For the therapeutic evaluation and pathogenesis study of dry eye, we established an in vitro three-dimensional (3D) coculture model for the ocular surface. It is composed of rabbit conjunctival epithelium and lacrimal gland cell spheroids, and recapitulates the aqueous and mucin layers of the tear film. We first investigated the culture conditions for both cell types to optimize their secretory functions, by employing goblet cell enrichment, air-lifting culture, and 3D spheroid formation techniques...
July 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28733512/new-booklet-on-rabbit-nutrition-for-retailers-and-vets
#13
(no author information available yet)
No abstract text is available yet for this article.
July 22, 2017: Veterinary Record
https://www.readbyqxmd.com/read/28733311/regional-septal-hinge-point-injury-contributes-to-adverse-biventricular-interactions-in-pulmonary-hypertension
#14
Eva Amalie Nielsen, Kenichi Okumura, Mei Sun, Vibeke E Hjortdal, Andrew N Redington, Mark K Friedberg
Death and morbidity in pulmonary arterial hypertension (PAH) are often due to right ventricular (RV) failure and associated left ventricular (LV) dysfunction. We investigated regional myocardial remodeling and function as the basis for adverse ventricular-ventricular interactions in experimental chronic RV pressure overload. Two distinct animal models were studied: A rabbit model of increased RV pressure-load through progressive pulmonary artery banding A rat model of monocrotaline (MCT)-induced pulmonary arterial hypertension (PAH)...
July 2017: Physiological Reports
https://www.readbyqxmd.com/read/28732031/breast-tissue-ablation-with-irreversible-electroporation-in-rabbits-a-safety-and-feasibility-study
#15
Wenlong Zhang, Wanning Wang, Wei Chai, Xiaomei Luo, Jiannan Li, Jian Shi, Liqi Bi, Lizhi Niu
BACKGROUND AND AIM: Irreversible electroporation (IRE) was confirmed to control several solid tumors effectively in vivo. Our preclinical study aimed to assess the feasibility and safety of IRE in the breast of rabbit. METHODS: Thirty New Zealand white rabbits were randomly divided into 3 groups of 10 rabbits (control group, IRE group A, and B). Two mono-electrode needles were inserted into the breast tissue by percutaneous puncture. Electrocardiogram and vital signs were monitored before, during, and after ablation...
2017: PloS One
https://www.readbyqxmd.com/read/28731330/mapping-functionally-important-residues-in-the-na-dicarboxylate-cotransporter-nadc1
#16
Claire Colas, Avner Schlessinger, Ana M Pajor
Transporters from the SLC13 family couple the transport of 2-4 Na+ ions with a di- or tricarboxylate, such as succinate or citrate. We have previously modeled mammalian SLC13 members including the Na+/dicarboxylate cotransporter NaDC1 (SLC13A2), based on a structure of the bacterial homolog VcINDY in an inward-facing conformation with one sodium ion bound at the Na1 site. In the present study, we modeled the outward-facing conformation of the rabbit and human NaDC1 (rbNaDC1 and hNaDC1) using an outward-facing model of VcINDY as a template, and identified residues in or near the putative Na2 and Na3 cation binding sites...
July 21, 2017: Biochemistry
https://www.readbyqxmd.com/read/28730829/mechanism-of-electrocardiographic-t-wave-flattening-in-diabetes-mellitus-experimental-and-simulation-study
#17
K A Sedova, J E Azarov, N V Arteyeva, A O Ovechkin, M A Vaykshnorayte, V A Vityazev, O G Bernikova, D N Shmakov, P Kneppo
In the present study we investigated the contribution of ventricular repolarization time (RT) dispersion (the maximal difference in RT) and RT gradients (the differences in RT in apicobasal, anteroposterior and interventricular directions) to T-wave flattening in a setting of experimental diabetes mellitus. In 9 healthy and 11 diabetic (alloxan model) open-chest rabbits, we measured RT in ventricular epicardial electrograms. To specify the contributions of apicobasal, interventricular and anteroposterior RT gradients and RT dispersion to the body surface potentials we determined T-wave voltage differences between modified upper- and lower-chest precordial leads (T-wave amplitude dispersions, TWAD)...
July 18, 2017: Physiological Research
https://www.readbyqxmd.com/read/28730738/the-effects-of-aspirated-thickened-water-on-survival-and-pulmonary-injury-in-a-rabbit-model
#18
Nogah Nativ-Zeltzer, Maggie A Kuhn, Denise M Imai, Ryan P Traslavina, Amanda S Domer, Juliana K Litts, Brett Adams, Peter C Belafsky
OBJECTIVES/HYPOTHESIS: Liquid thickeners are one of the most frequently utilized treatment strategies for persons with oropharyngeal swallowing dysfunction. The effect of commercially available thickeners on lung injury is uncertain. The purpose of this study was to compare the effects of aspiration of water alone, xanthan gum (XG)-thickened water, and cornstarch (CS)-thickened water on survival and lung morphology in a rabbit model. STUDY DESIGN: Animal model. Prospective small animal clinical trial...
July 21, 2017: Laryngoscope
https://www.readbyqxmd.com/read/28730387/dynamics-of-the-pro-oxidant-antioxidant-system-parameters-in-wound-discharge-and-plasma-in-experimental-purulent-wound-during-its-technological-liquid-phase-treatment
#19
I M Bykov, A A Basov, V V Malyshko, S S Dzhimak, S R Fedosov, A V Moiseev
The dynamics of the pro-oxidant/antioxidant system of rabbits was studied at the local (in exudate) and systemic (blood) levels during therapy of purulent wounds in liquid medium with the use of a programmed device and the efficiency of the proposed technological method was compared with that of classical methods for the treatment of these wounds. More rapid recovery of the indicators of the pro-oxidant/antioxidant system to physiological values in the postoperative period was observed after treatment by the proposed method (7 days vs...
July 18, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28730231/chondroprotective-effects-of-pulsed-shortwave-therapy-in-rabbits-with-experimental-osteoarthritis
#20
Rodica Ana Ungur, Adrian Florea, Alexandru Flaviu Tăbăran, Iuliu Călin Scurtu, Ioan Onac, Ileana Monica Borda, László Irsay, Viorela Mihaela Ciortea, Eleonora Dronca, Mihnea Tudor Zdrenghea, ŞoimiŢa Mihaela Suciu
INTRODUCTION: Osteoarthritis (OA) represents a public health challenge since the pathogenic treatment, able to induce cartilage regeneration, still remains unknown. Ageing of the population and increasing OA prevalence have led to a lot of research, aiming to identify treatments acting on chondrocytes that play a determinant role in cartilage degeneration÷regeneration balance. Pulsed shortwave therapy (with the classical application form - Diapulse) is a physiotherapy method with anabolic effects demonstrated on nervous, conjunctive and vascular tissues, but its effects on OA cartilage are not known...
2017: Romanian Journal of Morphology and Embryology, Revue Roumaine de Morphologie et Embryologie
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