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Zhong-Hua Fu, Guang-Hua Guo, Zhen-Fang Xiong, Xincheng Liao, Ming-Zhuo Liu, Jinhua Luo
The aim of the present study was to determine the effects of early anticoagulation treatment on severe burns complicated by inhalation injury in a rabbit model. Under anesthetization, an electrical burns instrument (100˚C) was used to scald the backs of rabbits for 15 sec, which established a 30% III severe burns model. Treatment of the rabbits with early anticoagulation effectively improved the severe burns complicated by inhalation injury‑induced lung injury, reduced PaO2, PaCO2 and SPO2 levels, suppressed the expression of tumor necrosis factor‑α, interleukin (IL)‑1β and IL‑6, and increased the activity of IL‑10...
September 20, 2017: Molecular Medicine Reports
C Spahr, T Knauf-Witzens, T Vahlenkamp, R G Ulrich, R Johne
Hepatitis E is a human disease mainly characterized by acute liver illness, which is caused by infection with the hepatitis E virus (HEV). Large hepatitis E outbreaks have been described in developing countries; however, the disease is also increasingly recognized in industrialized countries. Mortality rates up to 25% have been described for pregnant women during outbreaks in developing countries. In addition, chronic disease courses could be observed in immunocompromised transplant patients. Whereas the HEV genotypes 1 and 2 are mainly confined to humans, genotypes 3 and 4 are also found in animals and can be zoonotically transmitted to humans...
September 24, 2017: Zoonoses and Public Health
Gregory R Dion, Peter A Benedict, Milan R Amin, Ryan C Branski
OBJECTIVES/HYPOTHESIS: A secure airway is critical to study obstructive disorders of the larynx and trachea in preclinical models. Tracheostomy has been described in rabbits, swine, canines, and other mammals using tracheostomy tubes or permanent stomas. No studies specifically evaluated morbidity and mortality associated with these models, and existing studies using tracheostomy make little mention of tracheostomy-related complications. We assessed the management, complications, and mortality associated with tracheostomy in a rabbit model that has recently gained significant attention...
September 25, 2017: Laryngoscope
Li-Jun Cui, Di-Chen Li, Jian Liu, Lei Zhang, Yao Xing
AIM: To evaluate the intraocular pressure (IOP) control of an artificial trabeculum drainage system (ATDS), a newly designed glaucoma drainage device, and postoperative complications in normal rabbit eyes. METHODS: Pressure drops in air and fluid of 30 ATDS were measured after being connected to a closed manometric system. Twenty of them were then chosen and implanted randomly into the eyes of 20 rabbits. Postoperative slit-lamp, gonioscopic examination and IOP measurements were recorded periodically...
2017: International Journal of Ophthalmology
Jun Zhang, Nalei Zhou, Bin Zhang, Jingxue Ma
BACKGROUND: This study aimed to explore the inhibitory effect of biodegradable scleral plugs containing curcumin on rabbits with proliferative vitreoretinopathy (PVR). MATERIALS AND METHODS: The biodegradable scleral plugs containing curcumin were prepared by dissolving PLGA [poly(lactide-co-glycolide)] and curcumin. In total, 30 rabbits were divided into 2 groups: the model group received a vitreous injection of self-blood, and the treatment group received a vitreous injection of self-blood plus biodegradable scleral implants containing 1...
September 23, 2017: Ophthalmic Research
M K Kok, J K Chambers, N Ushio, A Watamori, Y Miwa, H Nakayama, K Uchida
Trichoblastoma is the most common skin tumour in the rabbit. The aim of the present study was to characterize the histological and immunohistochemical features of trichoblastoma in 27 rabbits. Common sites of tumour occurrence were the neck (6/30, 20%), head (5/30, 16.7%), flank (4/30, 13.3%) and hindlimb (4/30, 13.3%). Histologically, rabbit trichoblastoma was categorized into ribbon (10/30, 33.3%), trabecular (8/30, 26.7%) and mixed types (12/30, 40%). The tumour tissue showed close interaction with the surrounding stroma where prominent fibroblastic aggregation, known as papillary mesenchymal bodies, was frequently observed (24/30; 80%)...
August 2017: Journal of Comparative Pathology
Mingchun Gao, Ying Liu, Yongli Guo, Yujiao Wang, Haiyue Dai, Zhifeng Song, Junwei Wang, Wenyu Han
The multigene family of rabbit IFN-α (RbIFN-α) is located on chromosome 1, which shows seven functional genes in type I IFN locus. A novel RbIFN-α that remains unlocated in the rabbit genome was amplified and designated as the first novel rabbit IFN-α (RbIFN-αNov1), which possesses the typical molecular characteristics of type I IFNs and could be induced in RK-13 cells and peripheral blood mononuclear cells. After the mature peptide of RbIFN-αNov1 was expressed, its antiviral activity, physicochemical characteristics, and cytotoxicity were determined in vitro...
September 20, 2017: Developmental and Comparative Immunology
Jamal Bamoulid, Thomas Crepin, Emilie Gaiffe, Caroline Laheurte, Bruno Moulin, Luc Frimat, Philippe Rieu, Christiane Mousson, Antoine Durrbach, Anne-Elisabeth Heng, Jean-Michel Rebibou, Philippe Saas, Cécile Courivaud, Didier Ducloux
Broad T cell depletion by polyclonal anti-thymocyte globulins (ATG) has been used for many years as a part of immunosuppressive treatment in transplantation. Currently, two different ATG are used in clinical practice, Thymoglobulin and Grafalon. Due to differences in the immunization source, these products contain different specificities and quantity of antibodies. These differences may have clinical consequences. We conducted a nested study in a large prospective multicentric cohort of kidney transplant to determine whether Grafalon-treated and Thymoglobulin-treated patients experience different lymphocyte reconstitution and clinical outcomes...
September 20, 2017: Transplant Immunology
K P Dalton, A Podadera, V Granda, I Nicieza, D Del Llano, R González, J R de Los Toyos, M García Ocaña, F Vázquez, J M Martin Alonso, J M Prieto, F Parra, R Casais
The emergence and rapid spread of variant of the rabbit hemorrhagic disease virus (RHDV2) require new diagnostic tools to ensure that efficient control measures are adopted. In the present study, a specific sandwich enzyme-linked immunosorbent assay (ELISA) for detection of RHDV2 antigens in rabbit liver homogenates, based on the use of an RHDV2-specific monoclonal antibody (Mab) 2D9 for antigen capture and an anti-RHDV2 goat polyclonal antibody (Pab), was developed. This ELISA was able to successfully detect RHDV2 and RHDV2 recombinant virions with high sensitivity (100%) and specificity (97...
September 20, 2017: Journal of Virological Methods
Xavier Ligneau, Rashmi R Shah, Isabelle Berrebi-Bertrand, Gary R Mirams, Philippe Robert, Laurent Landais, Pierre Maison-Blanche, Jean-François Faivre, Jeanne-Marie Lecomte, Jean-Charles Schwartz
PURPOSE: We evaluated the concordance of results from two sets of nonclinical cardiovascular safety studies on pitolisant. METHODS: Nonclinical studies envisaged both in the ICH S7B guideline and Comprehensive in vitroProarrhythmia Assay (CiPA) initiative were undertaken. CiPA-initiative studies included in vitro ion channels and stem cell-derived human ventricular myocyte studies as well asin silico modelling of results to simulate human ventricular electrophysiology...
September 23, 2017: British Journal of Pharmacology
Steven Van Cruchten, Vanessa Vrolyk, Marie-France Perron Lepage, Marie Baudon, Hélène Voute, Sabine Schoofs, Julius Haruna, Marie-Odile Benoit-Biancamano, Benoît Ruot, Karel Allegaert
In this review paper, literature data on pre- and postnatal eye development are compared between humans and nonclinical species that are commonly used for human safety assessment, namely, mouse, rat, rabbit, dog, minipig, and nonhuman primates. Some new data on rat and minipig ocular development are also included. This compiled information can be helpful for species selection in juvenile toxicity studies or assist in the interpretation of (non)clinical data during pediatric drug development. Despite some differences in developmental windows and anatomical peculiarities, such as the lack of a fovea centralis in nonprimate species or the presence of a nictitating membrane in some nonclinical species, the functioning and development of the eye is strikingly similar between humans and other mammals...
September 22, 2017: Birth defects research
L Wen, A Mao, Z Fan, W Li, Q Xiao, Q Liu, J Xie, K He
Porcine circovirus-like virus P1, a novel single-stranded circular DNA virus, is a potential causative agent of post-weaning multisystemic wasting syndrome in swine. In this study, we detected P1 strains in serum samples from cattle, goats and rabbits suffering different clinical symptoms, in Jiangsu Province, China. We sequenced the whole genomes of six P1 strains, which showed 99.4%-100% nucleotide sequence similarity and were closely related to other porcine-origin P1 isolates. The sequences of six non-porcine-origin P1 viruses were most closely phylogenetically related to other porcine-origin P1 sequences...
September 21, 2017: Transboundary and Emerging Diseases
Lin Wang, W Gong, H Fu, M Li, Y Zhang, Z Luo, Q Xu, L Wang
A novel genotype of hepatitis E virus (HEV) has been discovered in ferrets and mink worldwide. However, the situation in China is unknown. Diverse HEV RNA belonging to HEV genotype C2 was detected in this study from 17.5% (18/103) of the laboratory ferrets and 1.9% (2/107) of farmed mink in China. Phylogenetic analysis showed that the ferret HEV strains clustered into three groups with known strains detected from the United States and the Netherlands. Attempted transmission of ferret HEV to two rabbits was not successful...
September 22, 2017: Transboundary and Emerging Diseases
Lianna Ferrari Jorge, Julio C Francisco, Nelson Bergonse, Cristina Baena, Katherine Athayde T Carvalho, Eltyeb Abdelwahid, Jose Rocha Faria Neto, Luiz Felipe Pinho Moreira, Luiz Cesar Guarita-Souza
BACKGROUND: Surgical correction of tracheal stenosis is still a complex and challenging procedure. Acellular human amniotic membranes (AHAM) represent a promising biomaterial source for tissue regeneration. The aim of this study was to evaluate whether AHAM grafts improve tissue regeneration of the trachea in a rabbit model of tracheostomy. METHODS: Twenty rabbits were randomized into two groups. Animals in the control group underwent surgical tracheostomy only and animals in the AHAM group underwent surgical tracheostomy and received an AHAM graft that covered the defect site...
September 22, 2017: Journal of Tissue Engineering and Regenerative Medicine
Y Z Li, X D Hu, X M Lai, Y F Li, Y Lei
BACKGROUND: Development of drug therapies and other techniques for wound care have resulted in significant improvement of the cure rate and shortening of the healing time for wounds. A modified technique of regulated oxygen-enriched negative pressure-assisted wound therapy (RO-NPT) has been reported. AIM: To evaluate the efficacy and impact of RO-NPT on wound recovery and inflammation. METHODS: Infected wounds were established on 40 adult female white rabbits, which were then randomized to one of four groups: O2 group, regulated negative pressure-assisted wound therapy (RNPT) group, regulated oxygen-enriched negative pressure-assisted wound therapy (RO-NPT) group and healthy control (HC) group...
September 21, 2017: Clinical and Experimental Dermatology
Miriam Martínez-Ramírez, Cristóbal Flores-Castillo, L Gabriela Sánchez-Lozada, Rocío Bautista-Pérez, Elizabeth Carreón-Torres, José Manuel Fragoso, José Manuel Rodriguez-Pérez, Fernando E García-Arroyo, Victoria López-Olmos, María Luna-Luna, Gilberto Vargas-Alarcón, Martha Franco, Oscar Pérez-Méndez
The potential cause-effect relationship between uric acid plasma concentrations and HDL functionality remains elusive. Therefore, this study aimed to explore the effect of oxonic acid (OA)-induced hyperuricemia on the HDL size distribution, lipid content of HDL subclasses, and apo AI turnover, as well as HDL functionality in New Zealand white rabbits. Experimental animals received OA 750 mg/kg/day by oral gavage during 21 days. The HDL-apo AI fractional catabolic rate (FCR) was determined by exogenous labeling with (125)I, and HDL subclasses were determined by sequential ultracentrifugation and PAGE...
September 22, 2017: Lipids
Jun Song, Dongshan Yang, Jinxue Ruan, Jifeng Zhang, Yuqing Eugene Chen, Jie Xu
Immunodeficient mice have been used predominantly in biomedical research. Realizing that large animal species may have an enhanced ability to predict clinical outcome relative to mice, we worked to develop immunodeficient rabbits by CRISPR/Cas9. We first demonstrated that multiplex embryo transfer efficiently produced multiple lines of single-gene mutant (SGM) founders. Embryos microinjected with single sgRNA targeting FOXN1, RAG2, IL2RG or PRKDC were pooled for embryo transfer. As few as three recipients were used to produce twenty SGM founders for four genes...
September 22, 2017: Scientific Reports
Mirela Curin, Milena Weber, Gerhard Hofer, Danijela Apostolovic, Walter Keller, Renate Reininger, Ines Swoboda, Susanne Spitzauer, Margit Focke-Tejkl, Marianne van Hage, Rudolf Valenta
Immunoglobulin E (IgE)-associated allergy affects more than 25% of the population. Can f 1 is the major dog allergen associated with respiratory symptoms but the epitopes recognized by allergic patients IgE on Can f 1 are unknown. To characterize IgE epitopes of Can f 1 recognized by dog allergic patients, six overlapping peptides spanning the Can f 1 sequence were synthesized. In direct IgE epitope mapping experiments peptides were analyzed for IgE reactivity by dot blot and Enzyme-linked immunosorbent assay (ELISA) with sera from dog allergic patients...
September 22, 2017: Scientific Reports
Anna B Pawlowska, Jerry C Cheng, Nicole A Karras, Weili Sun, Leo D Wang, Alison D Bell, Lisa Gutierrez, Joseph Rosenthal
Allogeneic stem cell transplantation (HCT) is curative in patients with severe sickle cell disease (SCD) but a significant number of patients lack a HLA-identical sibling or matched unrelated donor. Mismatched related (haploidentical) HCT with post-transplant cyclophosphamide (PTCY) allows expansion of the donor pool but is complicated by high rates of graft failure. In this report, we describe a favorable haploidentical HCT approach in a limited cohort of sickle cell patients with significant co-morbidities...
September 19, 2017: Biology of Blood and Marrow Transplantation
A Kolesarova, N Maruniakova, A Kadasi, M Halenar, M Marak, A V Sirotkin
T-2 toxin and its metabolite HT-2 toxin are one of the most toxic mycotoxins of type A-trichothecenes, which are produced mainly by Fusarium species. Therefore, study of Fusarium toxins T-2 toxin and HT-2 toxin is an essential issue because they could also play role in failures of reproductive functions as well as endocrine system of domestic animals. Assessment of the effect of A-trichothecene mycotoxin HT-2 toxin alone or combined with insulin-like growth factor (IGF-I), leptin and ghrelin on estradiol secretion by rabbit ovarian fragments in vitro was done...
September 22, 2017: Physiological Research
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