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https://www.readbyqxmd.com/read/29793112/3d-printed-membrane-as-an-alternative-to-amniotic-membrane-for-ocular-surface-conjunctival-defect-reconstruction-an-in-vitro-in-vivo-study
#1
Shima Dehghani, Morteza Rasoulianboroujeni, Hamed Ghasemi, Saeed Heidari Keshel, Zohreh Nozarian, Mohammad Naser Hashemian, Mehran Zarei-Ghanavati, Golshan Latifi, Reza Ghaffari, Zhanfeng Cui, Hua Ye, Lobat Tayebi
BACKGROUND: The aim of this study was to evaluate the surgical handling and clinical applicability of a specific 3D-printed membrane design fabricated using a gelatin, elastin and sodium hyaluronate blend for conjunctival reconstruction and compare it with amniotic membrane (AM), which is normally used in such surgeries. METHODS: 3D printing technique was employed to fabricate the membrane based on gradient design. Prior to printing, rheometry was employed to optimize the ink composition...
May 11, 2018: Biomaterials
https://www.readbyqxmd.com/read/29792919/seeing-the-light-shifting-from-wild-rhizomes-to-extraction-of-active-ingredients-from-above-ground-parts-of-paris-polyphylla-var-yunnanensis
#2
Xu-Jie Qin, Wei Ni, Chang-Xiang Chen, Hai-Yang Liu
ETHNOPHARMACOLOGICAL RELEVANCE: The dried rhizomes of Paris polyphylla var. yunnanensis are widely used in traditional Chinese medicine (TCM) as hemostatic, antitumor, and antimicrobial agents. More than 70 Chinese patent medicines are based on P. polyphylla var. yunnanensis rhizomes. Steroidal saponins are considered as the main active ingredients of these rhizomes. However, wild populations of P. polyphylla var. yunnanensis are greatly threatened due to the illegal wild harvest and over-utilization of the rhizomes...
May 21, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29792716/options-to-improve-the-quality-of-kidney-grafts-from-expanded-criteria-donors-experimental-study
#3
J Molacek, V Opatrný, V Treska, R Matejka, O Hes
INTRODUCTION: There is a worldwide discrepancy between the number of donors and the number of organs needed for transplantation, leading to certain expansion of criteria, resulting in acceptance of donor organs. This means that organs with worse parenchymal qualities may be harvested for transplantation. One possibility, how to ameliorate the quality of these organs, is to change the concept of their preservation during cold ischemia or even after sudden circulatory arrest, prior to the collection of the organ itself...
2018: Rozhledy V Chirurgii: Měsíčník Československé Chirurgické Společnosti
https://www.readbyqxmd.com/read/29792548/comparison-of-the-ocular-penetration-and-pharmacokinetics-between-natamycin-and-voriconazole-after-topical-instillation-in-rabbits
#4
Xin Zhao, Yang Tong, Xiaotong Wang, Xian Zhang, Shichao Chen, Hong Lu
PURPOSE: To investigate the ocular penetration of natamycin (NAT) and voriconazole (VRC) after topical instillation in New Zealand white rabbits using simplified liquid chromatography-tandem mass spectrometry (LC-MS/MS) and high-performance liquid chromatography. METHODS: Seventy-eight healthy rabbits were randomly divided into 3 groups. In the first 2 groups, 72 rabbits were used for single-dose testing (36 for NAT, 36 for VRC), in which 50 μL of 5.0% NAT or 1...
May 24, 2018: Journal of Ocular Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/29792369/gene-delivery-in-the-cornea-in-vitro-ex-vivo-evaluation-of-solid-lipid-nanoparticle-based-vectors
#5
Mónica Vicente-Pascual, Andrea Albano, María Á Solinís, Loredana Serpe, Alicia Rodríguez-Gascón, Federica Foglietta, Elisabetta Muntoni, Josune Torrecilla, Ana Del Pozo-Rodríguez, Luigi Battaglia
AIM: Inflammation is a process that underlies sight-threatening ocular surface diseases, and gene supplementation with the plasmid that encodes for p-IL10 will allow the sustained de novo synthesis of the cytokine to occur in corneal cells, and provide a long-term anti-inflammatory effect. This work describes the development of solid lipid nanoparticle systems for the delivery of p-IL10 to transfect the cornea. RESULTS: In vitro, vectors showed suitable features as nonviral vectors (size, ζ-potential, DNA binding, protection and release), and they were able to enter and transfect human corneal epithelial cells...
May 24, 2018: Nanomedicine
https://www.readbyqxmd.com/read/29792229/characterization-of-medle-1-a-protein-in-early-development-of-cryptosporidium-parvum
#6
Jilan Fei, Haizhen Wu, Jiayuan Su, Chanchan Jin, Na Li, Yaqiong Guo, Yaoyu Feng, Lihua Xiao
BACKGROUND: Cryptosporidium spp. are important diarrhea-causing pathogens in humans and animals. Comparative genomic analysis indicated that Cryptosporidium-specific MEDLE family proteins may contribute to host adaptation of Cryptosporidium spp., and a recent study of one member of this family, CpMEDLE-2 encoded by cgd5_4590, has provided evidence supporting this hypothesis. In this study, another member of the protein family, CpMEDLE-1 of Cryptosporidium parvum encoded by cgd5_4580, which is distinct from CpMEDLE-2 and has no signature motif MEDLE, was cloned, expressed and characterized to understand its function...
May 23, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29790689/detection-of-brucella-abortus-by-immunofluorescence-assay-using-anti-outer-membrane-protein-of-19-kda-antibody
#7
Elham Mohammadi, Mehdi Golchin
BACKGROUND: Brucellosis in humans is one of the most prevalent zoonotic diseases around the world with more than 500,000 new cases per year. It is a weakening disease that requires long-term antibiotic treatment, often resulting in permanent and disabling consequences. Outer membrane proteins (OMPs) of Brucella, which are non-lipopolysaccharide (LPS) antigens, have been used for the diagnostic kits of brucellosis and vaccine design. OBJECTIVES: The aim of this study was to identify Brucella abortus with an immunofluorescence (IF) test using an antibody against recombinant outer membrane protein (OMP) of 19 kDa of this bacterium...
May 15, 2018: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/29790688/loganic-acid-and-anthocyanins-from-cornelian-cherry-cornus-mas-l-fruits-modulate-diet-induced-atherosclerosis-and-redox-status-in-rabbits
#8
Tomasz Sozański, Alicja Z Kucharska, Stanisław Dzimira, Jan Magdalan, Dorota Szumny, Agnieszka Matuszewska, Beata Nowak, Narcyz Piórecki, Adam Szeląg, Małgorzata Trocha
BACKGROUND: Cornelian cherry (Cornus mas L.) is a plant growing in southeast Europe, in the past used in folk medicine. There are many previous publications showing the preventive effects of (poly)phenolic compounds, especially anthocyanins, on cardiovascular diseases, but there is a lack of studies comparing the effects of (poly)phenolics and other constituents of fruits. OBJECTIVES: We have attempted to determine if iridoids and anthocyanins from cornelian cherry fruits may affect the formation of atherosclerotic plaques in the aorta as well as lipid peroxidation and oxidative stress in the livers of cholesterol-fed rabbits...
April 25, 2018: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/29790645/fibrinogen-prothrombin-time-derived-method-is-not-useful-in-patients-anticoagulated-with-low-molecular-weight-heparins-or-rivaroxaban
#9
C Duboscq, M Martinuzzo, J M Ceresetto, M Lopez, L Barrera, J Oyhamburu, G Stemmelin
BACKGROUND: Fibrinogen prothrombin time derived method (FIBPT-d) with photo-optical coagulometers is easy and economical. However, there are few reports on the behavior of this test on samples from patients anticoagulated with direct oral anticoagulants (DOACs) or low molecular weight heparin (LMWH). OBJECTIVE: To compare fibrinogen results obtained by Clauss method (FIB C) and FIBPT-d with two thromboplastins in anticoagulated patients. POPULATION: 295 consecutive anticoagulated patients: 99 vitamin K antagonist (VKA), 49 unfractionated heparin (UFH), 47 LMWH, 50 rivaroxaban and 50 dabigatran; and 100 healthy controls (NC)...
May 23, 2018: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/29790189/the-identification-of-a-novel-antibody-for-cd133-using-human-antibody-phage-display
#10
Paige M Glumac, Colleen L Forster, Hong Zhou, Paari Murugan, Shilpa Gupta, Aaron M LeBeau
BACKGROUND: The transmembrane glycoprotein CD133 is believed to be a marker of adult prostate stem cells and cancer stem/initiating cells. Investigating the role of CD133 in the normal biology of the prostate and in cancer is complicated by the lack of a sensitive and accurate antibody for its detection. Here, we describe the characterization of a unique antibody identified using human antibody phage display that can recognize CD133 in both formalin-fixed tissues and cell lines. METHODS: A human single-chain variable fragment (scFv) antibody phage display library possessing a diversity of 8 × 109 was screened against fully glycosylated recombinant CD133...
May 22, 2018: Prostate
https://www.readbyqxmd.com/read/29789565/the-wide-utility-of-rabbits-as-models-of-human-diseases
#11
REVIEW
Pedro J Esteves, Joana Abrantes, Hanna-Mari Baldauf, Lbachir BenMohamed, Yuxing Chen, Neil Christensen, Javier González-Gallego, Lorenzo Giacani, Jiafen Hu, Gilla Kaplan, Oliver T Keppler, Katherine L Knight, Xiang-Peng Kong, Dennis K Lanning, Jacques Le Pendu, Ana Lemos de Matos, Jia Liu, Shuying Liu, Ana M Lopes, Shan Lu, Sheila Lukehart, Yukari C Manabe, Fabiana Neves, Grant McFadden, Ruimin Pan, Xuwen Peng, Patricia de Sousa-Pereira, Ana Pinheiro, Masmudur Rahman, Natalie Ruvoën-Clouet, Selvakumar Subbian, Maria Jesús Tuñón, Wessel van der Loo, Michael Vaine, Laura E Via, Shixia Wang, Rose Mage
Studies using the European rabbit Oryctolagus cuniculus contributed to elucidating numerous fundamental aspects of antibody structure and diversification mechanisms and continue to be valuable for the development and testing of therapeutic humanized polyclonal and monoclonal antibodies. Additionally, during the last two decades, the use of the European rabbit as an animal model has been increasingly extended to many human diseases. This review documents the continuing wide utility of the rabbit as a reliable disease model for development of therapeutics and vaccines and studies of the cellular and molecular mechanisms underlying many human diseases...
May 22, 2018: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29789544/development-of-muscular-dystrophy-in-a-crispr-engineered-mutant-rabbit-model-with-frame-disrupting-ano5-mutations
#12
Tingting Sui, Li Xu, Yeh Siang Lau, Di Liu, Tingjun Liu, Yandi Gao, Liangxue Lai, Renzhi Han, Zhanjun Li
Limb girdle muscular dystrophy type 2L (LGMD2L) and Miyoshi myopathy type 3 (MMD3) are autosomal recessive muscular dystrophy caused by mutations in the gene encoding anoctamin-5 (ANO5), which belongs to the anoctamin protein family. Two independent lines of mice with complete disruption of ANO5 transcripts did not exhibit overt muscular dystrophy phenotypes; instead, one of these mice was observed to present with some abnormality in sperm motility. In contrast, a third line of ANO5-knockout (KO) mice with residual expression of truncated ANO5 expression was reported to display defective membrane repair and very mild muscle pathology...
May 22, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29789248/native-enthesis-preservation-versus-removal-in-rotator-cuff-repair-in-a-rabbit-model
#13
Wei Su, Xiaoxi Li, Song Zhao, Peng Shen, Shikui Dong, Jia Jiang, Jinzhong Zhao
PURPOSE: The purpose of this study was to explore whether preservation of native enthesis during rotator cuff repair (RCR) results in a histologically and biomechanically better rotator cuff-greater tuberosity (RC-GT) connection in an animal model. METHODS: Thirty-six New Zealand white rabbits were used in this study. The supraspinatus tendons were cut from the footprint to create a rotator cuff tear (RCT) on both shoulders, followed by immediate repair, with a 1 mm thick tendon stump remaining on the footprint...
May 19, 2018: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/29789008/pharmacological-effects-of-n-2-2-2-2-6-dichlorophenyl-amino-phenyl-acetyl-oxy-ethyl-hyaluronamide-diclofenac-etalhyaluronate-si-613-a-novel-sodium-hyaluronate-derivative-chemically-linked-with-diclofenac
#14
Keiji Yoshioka, Tomochika Kisukeda, Ryoji Zuinen, Yosuke Yasuda, Kenji Miyamoto
BACKGROUND: Osteoarthritis (OA) is the most common joint disorder worldwide and one of the leading causes of disability in the elderly. We have investigated the novel sodium hyaluronate derivative chemically linked with diclofenac (DF), diclofenac etalhyaluronate (SI-613), which is a potentially safer and more effective treatment for OA knee pain. In this study, we evaluated the pharmacological effects of SI-613 in experimental arthritis models. METHODS: We compared the analgesic and anti-inflammatory effects of intra-articularly administered SI-613, hyaluronic acid (HA), and of orally administered diclofenac sodium (DF-Na) in rat silver nitrate-induced arthritis model and rabbit antigen-induced arthritis model...
May 22, 2018: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/29788431/novel-treatment-of-cryptococcal-meningitis-via-neurapheresistm-therapy
#15
Gordon J Smilnak, Lefko T Charalambous, Drew Cutshaw, Alykhan M Premji, Charles D Giamberardino, Christi G Ballard, Andrew P Bartuska, Tiffany U Ejikeme, Huaxin Sheng, Laura Zitella Verbick, Blake A Hedstrom, Promila C Pagadala, Aaron R McCabe, John R Perfect, Shivanand P Lad
Cryptococcal meningitis (CM) has emerged as the most common life-threatening fungal meningitis worldwide. Current management involves a sequential, longitudinal regimen of antifungals; despite a significant improvement in survival compared to uniform mortality without treatment, this drug paradigm has not led to a consistent cure. NeurapheresisTM therapy, extracorporeal filtration of yeasts from cerebrospinal fluid (CSF) in infected hosts, is presented here as a novel, one-time therapy for CM. In vitro filtration of CSF through this platform yielded a five-log reduction in concentration of the yeast and a one-log reduction in its polysaccharide antigen over 24 hours...
May 17, 2018: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29787749/epidermal-hmgb1-activates-dermal-fibroblasts-and-causes-hypertrophic-scar-formation-in-reduced-hydration
#16
Jingling Zhao, Jianxing Yu, Yingbin Xu, Lei Chen, Fei Zhou, Qiyi Zhai, Jun Wu, Bin Shu, Shaohai Qi
High-mobility group box 1 (HMGB1) protein is a multifunctional cytokine involved in inflammatory reactions and is known to play a key role in tissue repair and fibrosis. However, the function of HMGB1 in fibrotic skin diseases, such as hypertrophic scar formation, remains unclear. In this study, HMGB1 was detected in the nuclei of epidermal cells in normal skin and had accumulated in the cytoplasm in hypertrophic scars. By establishing a keratinocyte-fibroblast co-culture and conditional medium treatment models, we found that a reduced hydration condition increased the expression and secretion of HMGB1 in keratinocytes, subsequently activating dermal fibroblasts...
May 19, 2018: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/29787746/two-photon-microscopy-of-fungal-keratitis-affected-rabbit-cornea-ex-vivo-using-moxifloxacin-as-a-labeling-agent
#17
Jun Ho Lee, Viet-Hoan Le, Seunghun Lee, Jin Hyoung Park, Jin Ah Lee, Hungwon Tchah, Sungjee Kim, Myoung Joon Kim, Ki Hean Kim
Two-photon microscopy (TPM) is a three dimensional (3D) microscopic technique based on nonlinear two-photon fluorescence, which has been tested as an alternative to reflectance confocal microscopy (RCM) for detecting fungal keratitis via optical imaging. Although TPM provided images with better contrast than RCM for fungal keratitis, its imaging speed was relatively low because of weak intrinsic signal. Moxifloxacin, a Food and Drug Administration (FDA)-approved antibiotic, was recently used as a cell-labeling agent for TPM...
May 19, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29786743/role-of-tgf%C3%A2-%C3%AE-1-expressed-in-bone-marrow%C3%A2-derived-mesenchymal-stem-cells-in-promoting-bone-formation-in-a-rabbit-femoral-defect-model
#18
Bing-Yin Sun, Bao-Xiang Zhao, Jie-Ying Zhu, Zheng-Ping Sun, Yong-An Shi, Feng Huang
Bone defects represent a major clinical and socioeconomic problem without suitable treatment options. Previous studies have shown that transforming growth factor β1 (TGF‑β1) is important in the development of various diseases. The present study aimed to investigate the therapeutic potential of rabbit bone marrow‑derived mesenchymal stem cells (BMSCs) expressing TGF‑β1 in the treatment of rabbit femoral defects. First, rabbit BMSCs were identified and cultured. TGF‑β1 was then stably overexpressed in the rabbit BMSCs by lentivirus transfection, which was expressed at a high level in the femoral defects treated with TGF‑β1‑overexpressing BMSCs, compared with PBS‑treated controls...
May 17, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29786671/mechanisms-of-action-and-cell-death-associated-with-clostridium-perfringens-toxins
#19
REVIEW
Mauricio A Navarro, Bruce A McClane, Francisco A Uzal
Clostridium perfringens uses its large arsenal of protein toxins to produce histotoxic, neurologic and intestinal infections in humans and animals. The major toxins involved in diseases are alpha (CPA), beta (CPB), epsilon (ETX), iota (ITX), enterotoxin (CPE), and necrotic B-like (NetB) toxins. CPA is the main virulence factor involved in gas gangrene in humans, whereas its role in animal diseases is limited and controversial. CPB is responsible for necrotizing enteritis and enterotoxemia, mostly in neonatal individuals of many animal species, including humans...
May 22, 2018: Toxins
https://www.readbyqxmd.com/read/29785833/species-differences-in-bile-acids-i-plasma-and-urine-bile-acid-composition
#20
Rhishikesh Thakare, Jawaher Abdullah Alamoudi, Nagsen Gautam, A David Rodrigues, Yazen Alnouti
Maintenance of bile acid (BA) homeostasis is essential to achieve their physiologic functions and avoid their toxic effects. The marked differences in BA composition between preclinical safety models and humans may play a major role in the poor prediction of drug-induced liver injury using preclinical models. We compared the composition of plasma and urinary BAs and their metabolites between humans and several animal species. Total BA pools and their composition varied widely among different species. Highest sulfation of BAs was observed in human and chimpanzee...
May 21, 2018: Journal of Applied Toxicology: JAT
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