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https://www.readbyqxmd.com/read/28231031/different-serological-profiles-to-cooccurring-pathogenic-and-nonpathogenic-caliciviruses-in-wild-european-rabbits-oryctolagus-cuniculus-across-australia
#1
Tanya E Cox, June Liu, Remy van de Ven, Tanja Strive
Rabbit hemorrhagic disease virus (RHDV) was released in Australia as a biocontrol agent for wild European rabbits ( Oryctolagus cuniculus ) in 1995-96; however, its effects were variable across Australia with the greatest population reductions seen in lower annual rainfall areas (<400 mm). There is speculation that the reduced effectiveness observed at higher annual rainfall sites is at least partially due to the presence of a nonpathogenic calicivirus (RCV-A1). The RCV-A1 is related to RHDV and confers partial and transient protection against lethal RHDV infection in laboratory tests...
February 23, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28230633/insights-into-cryotherapy-and-joint-bleeding-cryotherapy-and-hemophilia
#2
Roya Ravanbod, Giti Torkaman, Mehdi Hedayati, Mohammad Esmaeil Zandi Nezhad
There is some controversy over the use of cryotherapy. Low temperatures (Temp) could interfere with coagulation and increase the risk of bleeding. We sought to examine the effect of cryotherapy on joint swelling, temperature, friction, and inflammatory condition after experimental hemarthrosis. The left knee of 23 albino rabbits, 10 in heparin Ice, five in citrate Ice, four in heparin control, and four in citrate control were injected intraarticularly with 1 ml of blood. In total, four animals were considered to be in normal control group...
February 21, 2017: Blood Coagulation & Fibrinolysis: An International Journal in Haemostasis and Thrombosis
https://www.readbyqxmd.com/read/28230589/effects-of-local-and-systemic-zoledronic-acid-application-on-titanium-implant-osseointegration-an-experimental-study-conducted-on-two-surface-types
#3
Serkan Dundar, Ferhan Yaman, Orhan Gecor, Omer Cakmak, Mustafa Kirtay, Tuba Talo Yildirim, Tahir Karaman, Mehmet Emre Benlidayi
The aim of this study was to evaluate the effects of local and systemic zoledronic acid (ZA) applications on titaniumoksit ceramic blasted (TiO-CB)- and sandblasted large acid-grit (SLA)-surfaced titanium implant osseointegration. Twelve New Zealand White rabbits were used in the study, divided into 6 groups: the TiO-CB (TiO-CB-CNT) (n = 2) and SLA (SLA-CNT) (n = 2) control groups in which TiO-CB- and SLA-surfaced titanium implants were surgically inserted into rabbit tibias but no treatment was applied; the TiO-CB (TiO-CB-LZA) (n = 2) and SLA (SLA-LZA) (n = 2) local ZA groups in which 1 mL of normal saline solution containing 2 mg of ZA was injected into sockets and after this the implants were integrated; and the TiO-CB (TiO-CB-SZA) (n = 2) and SLA (SLA-SZA) (n = 2) systemic ZA groups in which a single infusion of 0...
February 22, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28229635/oral-administration-of-fumonisin-b1-and-t-2-individually-and-in-combination-affects-hepatic-total-and-mitochondrial-membrane-lipid-profile-of-rabbits
#4
A Szabó, J Szabó-Fodor, H Fébel, M Mézes, G Bajzik, M Kovács
Weaned rabbits were fed diets contaminated with 2 mg/kg diet T-2 toxin alone, or 10 mg/kg diet fumonisin B1 (FB1) alone, and both toxins in combination (2 + 10 mg/kg, respectively) compared to a toxin-free control diet. Samplings were performed after 4 weeks (blood and liver). Bodyweight of T-2-fed group was lower after 4 weeks; the liver weight was increased dramatically (threefold of control). Liver total phospholipids (PLs) provided slight alterations in the fatty acid (FA) composition; all three toxin-treated groups showed a decrease in palmitoleic acid (C16:1 n7) proportion...
September 2016: Physiol Int
https://www.readbyqxmd.com/read/28229424/three-dimensional-printed-scaffolds-with-multipotent-mesenchymal-stromal-cells-for-rabbit-mandibular-reconstruction-and-engineering
#5
Dongdong Fang, Michael Roskies, Mohamed-Nur Abdallah, Mohammed Bakkar, Jack Jordan, Li-Chieh Lin, Faleh Tamimi, Simon D Tran
Multipotent mesenchymal stromal cells (MSC) derived from both the bone marrow and adipose tissue possess the ability to differentiate into multiple cell lineages, regulate the immune function by secreting numerous bioactive paracrine factors, and hold great potential in cell therapy and tissue engineering. When combined with three-dimensional (3D) scaffolds, MSC can be used for bone defect reconstruction and engineering. This protocol describes the isolation of bone marrow mesenchymal stromal cells (BMMSC) and adipose-tissue derived stem cells (ADSC) from rabbits for subsequent seeding on tissue-engineered 3D-printed scaffolds and transplantation into a rabbit-model with the goal of repairing large osseous mandibular defects (one quarter of the lower jaw is removed surgically)...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28229307/investigation-of-osteogenic-activity-of-primary-rabbit-periosteal-cells-stimulated-by-multi-axial-tensile-strain
#6
Chih-Hao Chiu, Jun-Liang Liu, Chih-Hsuan Chang, Kin Fong Lei, Alvin Chao-Yu Chen
Periosteum-derived cells was indicated to respond to mechanical force and have stem cell potential capable of differentiating into multiple tissue. Investigation of osteogenic activity under mechanical stimulation is important to understand the therapeutic conditions of fracture healing. In this work, a cell culture platform was developed for respectively providing isotropic and anisotropic axial strain. Primary rabbit periosteal cells were isolated and cultured in the chamber. Multi-axial tensile strain was received and osteogenic activity was investigated by mRNA expressions of CBFA1 and OPN...
March 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28228932/a-clinically-relevant-in-vivo-model-for-the-assessment-of-scaffold-efficacy-in-abdominal-wall-reconstruction
#7
Jeffrey Cy Chan, Krishna Burugapalli, Yi-Shiang Huang, John L Kelly, Abhay Pandit
An animal model that allows for assessment of the degree of stretching or contraction of the implant area and the in vivo degradation properties of biological meshes is required to evaluate their performance in vivo. Adult New Zealand rabbits underwent full thickness subtotal unilateral rectus abdominis muscle excision and were reconstructed with the non-biodegradable Peri-Guard(®), Prolene(®) or biodegradable Surgisis(®) meshes. Following 8 weeks of recovery, the anterior abdominal wall tissue samples were collected for measurement of the implant dimensions...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/28228921/scuttle-flies-diptera-phoridae-inhabiting-rabbit-carcasses-confined-to-plastic-waste-bins-in-malaysia-include-new-records-and-an-undescribed-species
#8
Raja M Zuha, See Huong-Wen, R Henry L Disney, Baharudin Omar
Scuttle flies (Diptera: Phoridae) are small-sized insects of forensic importance. They are well known for diversified species and habitats, but in the context of forensic entomology, scuttle flies' inhabitance of corpses remains inadequately explored. With recent reports indicating the existence of more scuttle fly species possibly inhabiting these environments, a decomposition study using animal carcasses in enclosed environments was conducted. The aim was to record the occurrence of scuttle flies on rabbit carcasses placed in sealed plastic waste bins for a 40-day period...
January 2017: Tropical Life Sciences Research
https://www.readbyqxmd.com/read/28228733/maturation-of-airway-defensive-reflexes-is-related-to-development-of-feeding-behavior-during-growth-in-rabbits
#9
Laurianne Coutier-Marie, Iulia Ioan, Claude Bonabel, Bruno Demoulin, Anne-Laure Leblanc, Ludivine Debitu, Cyril Schweitzer, François Marchal, Silvia Demoulin-Alexikova
Introduction: Cough and expiration reflex are major lower airway defense mechanisms that have not been studied throughout development in relation with the feeding behavior. Aim: To describe airway defense reflexes evoked by mechanical stimulation of the trachea in developing rabbit pups. Material and Methods: Sixty one pups were allocated to 3 groups according to their feeding behavior: suckling (n = 22), weanling (n = 21) and weaning (n = 18) group. The incidence and sensitivity of defense reflexes triggered by mechanical tracheal stimulation were studied in anesthetized and tracheotomized animals...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28228181/characterization-and-functional-analysis-of-serpin-1-like-gene-from-oak-silkworm-antheraea-pernyi
#10
H M Yu, B J Zhu, Y Sun, G Q Wei, L Wang, C Qian, M Nadeem Abbas, C L Liu
Serpins are a broadly distributed family of proteases found in various organisms that play an important role in regulating the immune response. Here, we identified a serpin-1 gene from Antheraea pernyi that encodes a 279 amino acid protein with a molecular weight of 30.8 kDa. We expressed the recombinant Ap-serpin-1 protein in Escherichia coli and used the purified protein to prepare rabbit anti-Ap-serpin-1 polyclonal antibodies. We calculated the enzyme-linked immunosorbent assay titer of the antibody as 1:128000...
February 23, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/28227376/electrocardiogram-reconstruction-from-high-resolution-voltage-optical-mapping
#11
Ilija Uzelac, Conner Herndon, James Farmer, Flavio Fenton, Ilija Uzelac, Conner Herndon, James Farmer, Flavio Fenton, Ilija Uzelac, Conner Herndon, James Farmer, Flavio Fenton
Electrocardiogram recordings during opucal mapping experiments in heart tissue are commonly used tu monitor the health of the preparation and to obtain dominant frequencies during arrhythmic and defibrillatory studies. However the use of ECG reconstructed from optical mapping is seldom used and to date it has not been strictly validated. In this manuscript we present the first detailed validation and comparison of Optical Mapping ECG, or OM-ECG, with standard ECG recordings by calculating the electrostatic potential in space as a function of the voltage measured optically and describe the different approximations that can be used to obtain unipolar or bipolar ECG recordings...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227282/development-of-matlab-software-to-control-data-acquisition-from-a-multichannel-systems-multi-electrode-array
#12
Erik Messier, Behnaz Ghoraani, Erik Messier, Erik Messier, Behnaz Ghoraani
A Multichannel Systems (MCS) microelectrode array data acquisition (DAQ) unit is used to collect multichannel electrograms (EGM) from a Langendorff perfused rabbit heart system to study sudden cardiac death (SCD). MCS provides software through which data being processed by the DAQ unit can be displayed and saved, but this software's combined utility with MATLAB is not very effective. MCSs software stores recorded EGM data in a MathCad (MCD) format, which is then converted to a text file format. These text files are very large, and it is therefore very time consuming to import the EGM data into MATLAB for real-time analysis...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227089/low-energy-defibrillation-research-using-a-rabbit-ventricular-model-optimizing-the-potential-gradient-distribution-using-multiple-epicardial-electrodes
#13
Jianfei Wang, Lian Jin, Xiaomei Wu, Biao Song, Li Qian, Weiqi Wang, Jianfei Wang, Lian Jin, Xiaomei Wu, Biao Song, Li Qian, Weiqi Wang, Xiaomei Wu, Li Qian, Biao Song, Jianfei Wang, Weiqi Wang, Lian Jin
Cardiac potential gradient distribution directly affects defibrillation efficacy, and the electrode configuration that ensures optimal distribution is yet to be determined. In this study, a rabbit ventricular finite element conductor model containing blood perfusion in ventricular cavities was developed. The electric field was solved on the model by using 95% myocardial volume potential gradient higher than 5 V/cm as the successful defibrillation threshold (DFT). Multiple epicardial electrodes (MEE) protocols and a SCAN protocol were used to identify the optimum defibrillation method...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28226982/measurement-of-electrical-impedance-in-different-ex-vivo-tissues
#14
Davide Meroni, Dario Bovio, Pietro A Frisoli, Andrea Aliverti, Davide Meroni, Dario Bovio, Pietro A Frisoli, Andrea Aliverti, Dario Bovio, Andrea Aliverti, Davide Meroni, Pietro A Frisoli
Bioimpedance allows living tissues characterization and detection of pathological states. Although in previous years several methods have been proposed to assess bioimpedance, many instruments used in studies of living tissues characterization are commercial devices designed for the measurement of components or electronic circuits and therefore the measurement of biological tissues can be affected by electrical polarization. In order to test if electrical impedance spectroscopy may be helpful in providing further information about the structure and the properties of tissues, an impedance meter for living-tissues, able to avoid polarization, was developed...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28226544/kurtosis-as-a-statistical-approach-to-identify-the-pivot-point-of-the-rotor
#15
Shivaram P Arunachalam, Elizabeth M Annoni, Siva K Mulpuru, Paul A Friedman, Elena G Tolkacheva, Shivaram P Arunachalam, Elizabeth M Annoni, Siva K Mulpuru, Paul A Friedman, Elena G Tolkacheva, Elena G Tolkacheva, Paul A Friedman, Elizabeth M Annoni, Siva K Mulpuru, Shivaram P Arunachalam
Atrial fibrillation (AF) is the most common sustained cardiac arrhythmia that causes stroke affecting more than 2.3 million people in the US. Catheter ablation to terminate AF is successful for paroxysmal AF but suffers limitations with persistent AF patients as current mapping methods cannot identify AF active substrates outside of pulmonary vein region. In this work, we developed a novel Kurtosis based mapping technique that can accurately identify pivot points of the rotors that were induced in ex-vivo isolated rabbit heart...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28226464/modulation-of-cardiac-pacemaker-inter-beat-intervals-by-sinoatrial-fibroblasts-a-numerical-study
#16
Ariel Greisas, Sharon Zlochiver, Ariel Greisas, Sharon Zlochiver, Ariel Greisas, Sharon Zlochiver
The potential effect of sinoatrial fibroblasts on beat rate and variability of the cardiac pacemakers is not yet fully understood. Heterocellular coupling formation and fibroblast proliferation during diseased conditions may further signify the impact of those cells on sinoatrial node function. In this study we numerically modeled the impact of varying numbers of fibroblasts that are electrically coupled to a single pacemaker cell on several electrophysiological parameters. We employed cellular kinetics of the rabbit sinoatrial myocyte, and employed a range of potential gap junctional coupling between fibroblasts and myocytes...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28226224/different-electrophysiological-effects-of-the-levo-and-dextrorotatory-isomers-of-mexiletine-in-isolated-rabbit-cardiac-muscle
#17
Zsolt Gurabi, Bence Patocskai, Balázs Györe, László Virág, Péter Mátyus, Julius Gy Papp, András Varró, Istvan Koncz
Racemic mexiletine is a widely used antiarrhythmic agent which blocks sodium channels. The effects of R-(-) and S-(+) mexiletine stereoisomers on maximum rate of depolarization (Vmax), conduction time and repolarization have not yet been investigated in isolated cardiac preparations. We studied the effect of the R-(-) and S-(+) mexiletine on rabbit cardiac action potential parameters by using the conventional microelectrode technique. Both enantiomers at 20 µM of therapeutically and experimentally relevant concentration, significantly depressed the Vmax at fast heart rates (BCLs 300 - 700 ms)...
February 22, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28225913/ultrasound-as-a-safe-and-reliable-guidance-for-subarachnoid-puncture-in-rabbits
#18
Ivan Dias Fernandes Pereira, Roberto Bezerra Vital, Ronaldo Silva, Felipe Gilberto Valerini, Vania Maria de Vasconcelos Machado, Mariangela Esrher Marques, Helio Miot, Lais Helena Navarro, Eliana Marisa Ganem
Purpose: To evaluate a model for studying the toxicity in nervous tissue and meninges using ultrasound to guide needle insertion into the subarachnoid space of rabbits, with the objective of avoiding injuries triggered by the puncture and by intraneural injection of solutions. Methods: Forty-five adult female rabbits were divided into 3 groups (G): G1 underwent subarachnoid puncture, G2 underwent subarachnoid injection of saline solution and G3 underwent subarachnoid injection of 0...
January 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/28225889/roles-of-monocyte-chemotactic-protein-1-and-nuclear-factor-%C3%AE%C2%BAb-in-immune-response-to-spinal-tuberculosis-in-a-new-zealand-white-rabbit-model
#19
X H Guo, Z Bai, B Qiang, F H Bu, N Zhao
This study aimed to explore the roles of monocyte chemotactic protein 1 (MCP-1) and nuclear factor kappa B (NF-κB) in immune response to spinal tuberculosis in a New Zealand white rabbit model. Forty-eight New Zealand white rabbits were collected and divided into four groups: experimental group (n=30, spinal tuberculosis model was established), the sham group (n=15, sham operation was performed) and the blank group (n=3). The qRT-PCR assay and western blotting were applied to detect the mRNA and protein expressions of MCP-1 and NF-κB in peripheral blood...
February 20, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/28224451/pet-cta-detection-of-muscle-inflammation-related-to-cholesterol-crystal-emboli-without-arterial-obstruction
#20
M Hassan Pervaiz, Sridevi Durga, Abed Janoudi, Kevin Berger, George S Abela
BACKGROUND: PET/CTA was used to evaluate the effect of cholesterol crystal emboli (CCE) on muscle injury. Cholesterol crystals (CCs) released during plaque rupture travel downstream and lodge in muscle triggering inflammation and tissue injury. METHODS: Thigh muscles in three groups of rabbits (n = 22) were studied after intra-arterial injection of CCs, Group I (n = 10); polystyrene microspheres, Group II (n = 5); or normal saline, Group III (n = 7). After 48 hours, muscle inflammation and injury were measured by fluorodeoxy-glucose uptake using PET/CTA, serum tissue factor (TF), and creatinine phosphokinase (CPK)...
February 21, 2017: Journal of Nuclear Cardiology: Official Publication of the American Society of Nuclear Cardiology
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