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Bassem Y Sheikh, Md Moklesur Rahman Sarker, Muhamad Noor Alfarizal Kamarudin, Gokula Mohan
Despite various anticancer reports, antiproliferative and apoptosis inducing activity of citral in HCT116 and HT29 cells have never been reported. This study aimed to evaluate the cytotoxic and apoptosis inducing effects of citral in colorectal cancer cell lines. The citral-treated cells were subjected to MTT assay followed by flow cytometric Annexin V-FITC/PI, mitochondrial membrane potential and intracellular reactive oxygen species (ROS) determination. The apoptotic proteins expression was investigated by Western blot analysis...
December 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Amanda Phaelante, Luís Eduardo Rohde, Amanda Lopes, Virgílio Olsen, Santiago Alonso Leitão Tobar, Carolina Cohen, Nidiane Martinelli, Andréia Biolo, Felipe Dal-Pizzol, Nadine Clausell, Michael Andrades
The antioxidant N-acetycysteine can turn into a prooxidant molecule in presence of iron ions. Thus, our goal was to test if the association of N-acetylcysteine (NAC) and an iron chelator (deferoxamine--DFX) in a rodent model of acute myocardial infarction (AMI) improves cardiac function. Male Wistar rats were subjected to a SHAM surgery or AMI. The animals were randomized: vehicle, NAC (25 mg/kg for 28 days), DFX (40 mg/kg for 7 days), or NAC plus DFX (NAC plus DFX, respectively). Animals were killed 28 days after the AMI...
July 2015: Journal of Cardiovascular Translational Research
Christian Ziegler, Jürgen Finke, Carsten Grüllich
Rabbit anti-T-lymphocyte-globulin (ATG) is used for immunosuppression in organ and stem cell transplantation. The aim of this study was to investigate ATG-induced cell death compared to CD95-signaling of apoptosis. We measured features of cell death at the cell membrane, mitochondria, nuclei and caspase-3 cleavage. We used the following inhibitors: the caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp (O-Me)-fluoromethyl ketone (zVAD-fmk), the serine protease inhibitors 3,4 dichloroisocoumarin (DCI) and N-alpha-tosyl-L-lysinyl-chloromethylketone (TLCK) and the reducing agent N-acetycysteine (NAC)...
2016: Leukemia & Lymphoma
Seok-Jeong Oh, Hwa Kim, Yingqiu Liu, Hyo-Kyung Han, Kyenghee Kwon, Kyung-Hwa Chang, Kwangsik Park, Younghun Kim, Kyuhwan Shim, Seong Soo A An, Moo-Yeol Lee
A growing number of studies report that conventional cytotoxicity assays are incompatible with certain nanoparticles (NPs) due to artifacts caused by the distinctive characteristics of NPs. Lactate dehydrogenase (LDH) leakage assays have inadequately detected cytotoxicity of silver nanoparticles (AgNPs), leading to research into the underlying mechanism. When ECV304 endothelial-like umbilical cells were treated with citrate-capped AgNPs (cAgNPs) or bare AgNPs (bAgNPs), the plasma membrane was disrupted, but the LDH leakage assay failed to detect cytotoxicity, indicating interference with the assay by AgNPs...
March 21, 2014: Toxicology Letters
S Shoji, K Furuishi, S Misumi, T Miyazaki, M Kino, K Yamataka
Thiol and disulfide compounds were tested as an anti-HIV drug against transactivator (Tat)-mediated transactivation of HIV-1. Of all the compounds tested, thiamine disulfide, alpha-lipoic acid, and N-acetycysteine significantly depressed HIV-1 Tat activity. Thiamine disulfide alone in these compounds possessing anti-HIV-Tat activity markedly inhibited production of progeny HIV-1 in acute and chronic HIV-1-infected CEM at nontoxic concentrations of 500-1000 microM. Thiamine disulfide (500 microM) blocked 99...
November 30, 1994: Biochemical and Biophysical Research Communications
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