keyword
https://read.qxmd.com/read/37922575/hhex-and-prox1a-synergistically-dictate-the-hepatoblast-to-hepatocyte-differentiation-in-zebrafish
#21
JOURNAL ARTICLE
Qingxia Jin, Yuqing Hu, Yuqi Gao, Jiayi Zheng, Jun Chen, Ce Gao, Jinrong Peng
The specification of endoderm cells to prospective hepatoblasts is the starting point for hepatogenesis. However, how a prospective hepatoblast gains the hepatic fate remains elusive. Previous studies have shown that loss-of-function of either hhex or prox1a alone causes a small liver phenotype but without abolishing the hepatocyte differentiation, suggesting that absence of either Hhex or Prox1a alone is not sufficient to block the hepatoblast differentiation. Here, via genetic studies of the zebrafish two single (hhex-/- and prox1a-/- ) and one double (hhex-/- prox1a-/- ) mutants, we show that simultaneous loss-of-function of the hhex and prox1a two genes does not block the endoderm cells to gain the hepatoblast potency but abolishes the hepatic differentiation from the prospective hepatoblast...
October 30, 2023: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/37902497/response-of-lymphatic-endothelial-cells-to-combined-spatial-and-temporal-variations-in-fluid-flow
#22
JOURNAL ARTICLE
Eleftheria Michalaki, Vinay N Surya, Mariana Rodríguez-Hakim, Gerald G Fuller, Alexander R Dunn
One-way valves within lymphatic vessels are required for the efficient drainage of lymphatic fluids. Fluid flow is proposed to be a key cue in regulating both the formation and maintenance of lymphatic valves. However, to our knowledge, no previous study has systematically examined the response of LECs to the complex combination of spatially and temporally varying fluid flows that occur at lymphatic valves in vivo. We built an in vitro microfluidic device that reproduces key aspects of the flow environment found at lymphatic valves...
December 2023: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/37887322/eye-lens-organoids-made-simple-characterization-of-a-new-three-dimensional-organoid-model-for-lens-development-and-pathology
#23
JOURNAL ARTICLE
Matthieu Duot, Roselyne Viel, Justine Viet, Catherine Le Goff-Gaillard, Luc Paillard, Salil A Lachke, Carole Gautier-Courteille, David Reboutier
Cataract, the opacification of the lens, is the leading cause of blindness worldwide. Although effective, cataract surgery is costly and can lead to complications. Toward identifying alternate treatments, it is imperative to develop organoid models relevant for lens studies and drug screening. Here, we demonstrate that by culturing mouse lens epithelial cells under defined three-dimensional (3D) culture conditions, it is possible to generate organoids that display optical properties and recapitulate many aspects of lens organization and biology...
October 18, 2023: Cells
https://read.qxmd.com/read/37867430/prospero-homeobox-1-and-doublecortin-correlate-with-neural-damage-after-ischemic-stroke
#24
JOURNAL ARTICLE
Dong-Hun Lee, Eun Chae Lee, Sang-Won Park, Ji Young Le, Kee-Pyo Kim, Jae Sang Oh
OBJECTIVE: Markers of neuroinflammation during ischemic stroke are well characterized, but additional markers of neural damage are lacking. The study identified associations of behavioral disorders after stroke with histologic neural damage and molecular biological change. METHODS: 8-week-old, 25g male mice of the C57BL/6J strain were subjected to middle cerebral artery occlusion (MCAO) to induce ischemic stroke. The control group was a healthy wild type (WT), and the experimental group were designed as a low severity MCAO1 and a high severity MCAO2 based on post-stroke neurological scoring...
October 23, 2023: Journal of Korean Neurosurgical Society
https://read.qxmd.com/read/37789798/synthetic-hyaluronic-acid-coating-preserves-the-phenotypes-of-lymphatic-endothelial-cells
#25
JOURNAL ARTICLE
Sanjoy Saha, Fei Fan, Laura Alderfer, Francine Graham, Eva Hall, Donny Hanjaya-Putra
Lymphatic endothelial cells (LECs) play a critical role in the formation and maintenance of the lymphatic vasculature, which is essential for the immune system, fluid balance, and tissue repair. However, LECs are often difficult to study in vivo and in vitro models that accurately mimic their behaviors and phenotypes are limited. In particular, LECs have been shown to lose their lymphatic markers over time while being cultured in vitro , which reflect their plasticity and heterogeneity in vivo . Since LECs uniquely express lymphatic vessel endothelial hyaluronan receptor-1 ( LYVE-1 ), we hypothesized that surface coating with hyaluronic acid (HA) can preserve LEC phenotypes and functionalities...
October 4, 2023: Biomaterials Science
https://read.qxmd.com/read/37771785/detection-of-lineage-reprogramming-efficiency-of-tumor-cells-in-a-3d-printed-liver-on-a-chip-model
#26
JOURNAL ARTICLE
Zuyan Lu, Xiangwan Miao, Qianqian Song, Huifen Ding, Shiny Amala Priya Rajan, Aleksander Skardal, Konstantinos I Votanopoulos, Kerong Dai, Weixin Zhao, Baisong Lu, Anthony Atala
Background: The liver metastasis accompanied with the loss of liver function is one of the most common complications in patients with triple-negative breast cancers (TNBC). Lineage reprogramming, as a technique direct inducing the functional cell types from one lineage to another lineage without passing through an intermediate pluripotent stage, is promising in changing cell fates and overcoming the limitations of primary cells. However, most reprogramming techniques are derived from human fibroblasts, and whether cancer cells can be reversed into hepatocytes remains elusive...
2023: Theranostics
https://read.qxmd.com/read/37759531/lymphatic-defects-in-zebrafish-sox18-mutants-are-exacerbated-by-perturbed-vegfc-signaling-while-masked-by-elevated-sox7-expression
#27
JOURNAL ARTICLE
Silvia Moleri, Sara Mercurio, Alex Pezzotta, Donatella D'Angelo, Alessia Brix, Alice Plebani, Giulia Lini, Marialaura Di Fuorti, Monica Beltrame
Mutations in the transcription factor-coding gene SOX18 , the growth factor-coding gene VEGFC and its receptor-coding gene VEGFR3/FLT4 cause primary lymphedema in humans. In mammals, SOX18, together with COUP-TFII/NR2F2, activates the expression of Prox1 , a master regulator in lymphatic identity and development. Knockdown studies have also suggested an involvement of Sox18, Coup-tfII/Nr2f2, and Prox1 in zebrafish lymphatic development. Mutants in the corresponding genes initially failed to recapitulate the lymphatic defects observed in morphants...
September 19, 2023: Cells
https://read.qxmd.com/read/37730744/ve-cadherin-in-arachnoid-and-pia-mater-cells-serves-as-a-suitable-landmark-for-in-vivo-imaging-of-cns-immune-surveillance-and-inflammation
#28
JOURNAL ARTICLE
Josephine A Mapunda, Javier Pareja, Mykhailo Vladymyrov, Elisa Bouillet, Pauline Hélie, Petr Pleskač, Sara Barcos, Johanna Andrae, Dietmar Vestweber, Donald M McDonald, Christer Betsholtz, Urban Deutsch, Steven T Proulx, Britta Engelhardt
Meninges cover the surface of the brain and spinal cord and contribute to protection and immune surveillance of the central nervous system (CNS). How the meningeal layers establish CNS compartments with different accessibility to immune cells and immune mediators is, however, not well understood. Here, using 2-photon imaging in female transgenic reporter mice, we describe VE-cadherin at intercellular junctions of arachnoid and pia mater cells that form the leptomeninges and border the subarachnoid space (SAS) filled with cerebrospinal fluid (CSF)...
September 20, 2023: Nature Communications
https://read.qxmd.com/read/37693499/foxc1-and-foxc2-maintain-mitral-valve-endothelial-cell-junctions-extracellular-matrix-and-lymphatic-vessels-to-prevent-myxomatous-degeneration
#29
Can Tan, Shreya Kurup, Yaryna Dyakiv, Tsutomu Kume
BACKGROUND: Mitral valve (MV) disease including myxomatous degeneration is the most common form of valvular heart disease with an age-dependent frequency. Genetic evidence indicates mutations of the transcription factor FOXC1 are associated with MV defects, including mitral valve regurgitation. In this study, we sought to determine whether murine Foxc1 and its closely related factor, Foxc2 , are required in valvular endothelial cells (VECs) for the maintenance of MV leaflets, including VEC junctions and the stratified trilaminar extracellular matrix (ECM)...
August 30, 2023: bioRxiv
https://read.qxmd.com/read/37685269/identification-of-the-immune-landscapes-and-follicular-helper-t-cell-related-genes-for-the-diagnosis-of-age-related-macular-degeneration
#30
JOURNAL ARTICLE
Yao Yang, Zhiqiang Sun, Zhenping Li, Que Wang, Mingjing Yan, Wenlin Li, Kun Xu, Tao Shen
BACKGROUND: Age-related macular degeneration (AMD) is a progressive ocular ailment causing age-associated vision deterioration, characterized by dysregulated immune cell activity. Notably, follicular helper T (Tfh) cells have emerged as pivotal contributors to AMD pathogenesis. Nonetheless, investigations into Tfh-associated gene biomarkers for this disorder remain limited. METHODS: Utilizing gene expression data pertinent to AMD procured from the Gene Expression Omnibus (GEO) repository, we employed the "DESeq2" R software package to standardize and preprocess expression levels...
August 22, 2023: Diagnostics
https://read.qxmd.com/read/37676760/single-nuclei-multiomic-analyses-identify-human-cardiac-lymphatic-endothelial-cells-associated-with-coronary-arteries-in-the-epicardium
#31
JOURNAL ARTICLE
Stanislao Igor Travisano, Michael R M Harrison, Matthew E Thornton, Brendan H Grubbs, Thomas Quertermous, Ching-Ling Lien
Cardiac lymphatic vessels play important roles in fluid homeostasis, inflammation, disease, and regeneration of the heart. The developing cardiac lymphatics in human fetal hearts are closely associated with coronary arteries, similar to those in zebrafish hearts. We identify a population of cardiac lymphatic endothelial cells (LECs) that reside in the epicardium. Single-nuclei multiomic analysis of the human fetal heart reveals the plasticity and heterogeneity of the cardiac endothelium. Furthermore, we find that VEGFC is highly expressed in arterial endothelial cells and epicardium-derived cells, providing a molecular basis for the arterial association of cardiac lymphatic development...
September 6, 2023: Cell Reports
https://read.qxmd.com/read/37662289/progressive-plasticity-during-colorectal-cancer-metastasis
#32
A R Moorman, F Cambuli, E K Benitez, Q Jiang, Y Xie, A Mahmoud, M Lumish, S Hartner, S Balkaran, J Bermeo, S Asawa, C Firat, A Saxena, A Luthra, V Sgambati, K Luckett, F Wu, Y Li, Z Yi, I Masilionis, K Soares, E Pappou, R Yaeger, P Kingham, W Jarnagin, P Paty, M R Weiser, L Mazutis, M D'Angelica, J Shia, J Garcia-Aguilar, T Nawy, T J Hollmann, R Chaligné, F Sanchez-Vega, R Sharma, D Pe'er, K Ganesh
Metastasis is the principal cause of cancer death, yet we lack an understanding of metastatic cell states, their relationship to primary tumor states, and the mechanisms by which they transition. In a cohort of biospecimen trios from same-patient normal colon, primary and metastatic colorectal cancer, we show that while primary tumors largely adopt LGR5 + intestinal stem-like states, metastases display progressive plasticity. Loss of intestinal cell states is accompanied by reprogramming into a highly conserved fetal progenitor state, followed by non-canonical differentiation into divergent squamous and neuroendocrine-like states, which is exacerbated by chemotherapy and associated with poor patient survival...
August 21, 2023: bioRxiv
https://read.qxmd.com/read/37621616/retracted-effects-of-incrna-prox1-as1-on-proliferation-migration-invasion-and-apoptosis-of-lung-cancer-cells-by-regulating-mir-1305
#33
Journal Of Healthcare Engineering
[This retracts the article DOI: 10.1155/2022/9570900.].
2023: Journal of Healthcare Engineering
https://read.qxmd.com/read/37560809/fasting-sensitive-sumo-switch-on-prox1-controls-hepatic-cholesterol-metabolism
#34
JOURNAL ARTICLE
Ana Jimena Alfaro, Claudia Dittner, Janina Becker, Anne Loft, Amit Mhamane, Adriano Maida, Anastasia Georgiadi, Foivos-Filippos Tsokanos, Katarina Klepac, Claudia-Eveline Molocea, Rabih El-Merahbi, Karsten Motzler, Julia Geppert, Rhoda Anane Karikari, Julia Szendrödi, Annette Feuchtinger, Susanna Hofmann, Samir Karaca, Henning Urlaub, Mauricio Berriel Diaz, Frauke Melchior, Stephan Herzig
Accumulation of excess nutrients hampers proper liver function and is linked to nonalcoholic fatty liver disease (NAFLD) in obesity. However, the signals responsible for an impaired adaptation of hepatocytes to obesogenic dietary cues remain still largely unknown. Post-translational modification by the small ubiquitin-like modifier (SUMO) allows for a dynamic regulation of numerous processes including transcriptional reprogramming. We demonstrate that specific SUMOylation of transcription factor Prox1 represents a nutrient-sensitive determinant of hepatic fasting metabolism...
August 10, 2023: EMBO Reports
https://read.qxmd.com/read/37555328/prox1-inhibits-pdgf-b-expression-to-prevent-myxomatous-degeneration-of-heart-valves
#35
JOURNAL ARTICLE
Yen-Chun Ho, Xin Geng, Anna O'Donnell, Jaime Ibarrola, Amaya Fernandez-Celis, Rohan Varshney, Kumar Subramani, Zheila J Azartash-Namin, Jang Kim, Robert Silasi, Jill Wylie-Sears, Zahra Alvandi, Lijuan Chen, Boksik Cha, Hong Chen, Lijun Xia, Bin Zhou, Florea Lupu, Harold M Burkhart, Elena Aikawa, Lorin E Olson, Jasimuddin Ahamed, Natalia López-Andrés, Joyce Bischoff, Katherine E Yutzey, R Sathish Srinivasan
BACKGROUND: Cardiac valve disease is observed in 2.5% of the general population and 10% of the elderly people. Effective pharmacological treatments are currently not available, and patients with severe cardiac valve disease require surgery. PROX1 (prospero-related homeobox transcription factor 1) and FOXC2 (Forkhead box C2 transcription factor) are transcription factors that are required for the development of lymphatic and venous valves. We found that PROX1 and FOXC2 are expressed in a subset of valvular endothelial cells (VECs) that are located on the downstream (fibrosa) side of cardiac valves...
August 9, 2023: Circulation Research
https://read.qxmd.com/read/37543710/abnormal-chromatin-remodeling-caused-by-arid1a-deletion-leads-to-malformation-of-the-dentate-gyrus
#36
JOURNAL ARTICLE
Pei-Pei Liu, Shi-Ping Lu, Xiao Li, Gang-Bin Tang, Xiao Liu, Shang-Kun Dai, Lin-Fei Jiao, Xi-Wen Lin, Xing-Guo Li, Baoyang Hu, Jianwei Jiao, Zhao-Qian Teng, Chun-Sheng Han, Chang-Mei Liu
ARID1A, an SWI/SNF chromatin-remodeling gene, is commonly mutated in cancer and hypothesized to be a tumor suppressor. Recently, loss-of-function of ARID1A gene has been shown to cause intellectual disability. Here we generate Arid1a conditional knockout mice and investigate Arid1a function in the hippocampus. Disruption of Arid1a in mouse forebrain significantly decreases neural stem/progenitor cells (NSPCs) proliferation and differentiation to neurons within the dentate gyrus (DG), increasing perinatal and postnatal apoptosis, leading to reduced hippocampus size...
August 5, 2023: Cell Death and Differentiation
https://read.qxmd.com/read/37508533/prox1-suppresses-the-proliferation-of-breast-cancer-cells-via-direct-inhibition-of-c-myc-gene-expression
#37
JOURNAL ARTICLE
Artemis Michail, Dimitrios Gkikas, Dimitris Stellas, Valeria Kaltezioti, Panagiotis K Politis
Breast cancer is one of the most lethal malignancies in women worldwide and is characterized by rapid growth and low survival rates, despite advances in tumor biology and therapies. Novel therapeutic approaches require new insights into the molecular mechanisms of malignant transformation and progression. To this end, here, we identified Prox1 as a negative regulator of proliferation and tumor-related metabolism in breast cancer. In particular, we showed that breast tumors from human patients exhibited reduced levels of Prox1 expression, while high expression levels of Prox1 were associated with a favorable prognosis in breast cancer patients...
July 17, 2023: Cells
https://read.qxmd.com/read/37503058/zmiz1-is-a-novel-regulator-of-lymphatic-endothelial-cell-gene-expression-and-function
#38
Rajan K C, Nehal R Patel, Anoushka Shenoy, Joshua P Scallan, Mark Y Chiang, Maria J Galazo, Stryder M Meadows
Zinc Finger MIZ-Type Containing 1 (Zmiz1), also known as ZIMP10 or RAI17, is a transcription cofactor and member of the Protein Inhibitor of Activated STAT (PIAS) family of proteins. Zmiz1 is critical for a variety of biological processes including vascular development. However, its role in the lymphatic vasculature is unknown. In this study, we utilized human dermal lymphatic endothelial cells (HDLECs) and an inducible, lymphatic endothelial cell (LEC)-specific Zmiz1 knockout mouse model to investigate the role of Zmiz1 in LECs...
July 22, 2023: bioRxiv
https://read.qxmd.com/read/37503005/eye-lens-organoids-going-simple-characterization-of-a-new-3-dimensional-organoid-model-for-lens-development-and-pathology
#39
Matthieu Duot, Roselyne Viel, Justine Viet, Catherine Le Goff-Gaillard, Luc Paillard, Salil A Lachke, Carole Gautier-Courteille, David Reboutier
The ocular lens, along with the cornea, focuses light on the retina to generate sharp images. Opacification of the lens, or cataract, is the leading cause of blindness worldwide. Presently, the best approach for cataract treatment is to surgically remove the diseased lens and replace it with an artificial implant. Although effective, this is costly and can have post-surgical complications. Toward identifying alternate treatments, it is imperative to develop organoid models relevant for lens studies and anti-cataract drug screening...
July 12, 2023: bioRxiv
https://read.qxmd.com/read/37486381/characterization-of-the-soft-tissue-wall-lining-residual-periodontal-pockets-and-implications-in-periodontal-wound-healing
#40
JOURNAL ARTICLE
Evangelia Gousopoulou, Athina Bakopoulou, Dimitrios Laskaris, Epameinondas Gousopoulos, Danae A Apatzidou
AIM: To characterize the soft-tissue wall of remaining periodontal pockets for wound healing-related parameters versus healthy gingival crevices in the same individuals. MATERIALS AND METHODS: Gingival tissues collected from the diseased interface of pockets (GT biopsies) and from healthy gingival crevices (G biopsies) were subjected to RT2 -profiler PCR Array for wound healing-related markers and network analysis of differentially expressed genes. Lymphangiogenesis-related gene expression was determined by qRT-PCR...
July 24, 2023: Clinical Oral Investigations
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