keyword
https://read.qxmd.com/read/38450249/developmental-progression-of-lymphatic-valve-morphology-and-function
#1
JOURNAL ARTICLE
Michael J Davis, Scott D Zawieja, Ying Yang
Introduction: The bileaflet valves found in collecting lymphatic vessels and some veins are essential for maintaining a unidirectional flow, which is important for lymphatic and venous function. Under an adverse pressure gradient, the two leaflets tightly overlap to prevent backflow. Valves are proposed to share four main stages of development, based on images obtained from randomly oriented valves in fixed mouse embryos, with the best structural views obtained from larger venous valves. It is not known at what stage lymphatic valves (LVs) become functional (e...
2024: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/38433049/-clinicopathological-and-genetic-characteristics-of-common-lymphatic-malformations-in-superficial-soft-tissues
#2
JOURNAL ARTICLE
Q Y Liu, C X Li, D K Liu, S Xue, F F Fu, X S Zhu, L F Kong, C X Dong
Objective: To investigate the clinicopathological features, classification, and genetic characteristics of common lymphatic malformation (CLM) in superficial soft tissue. Methods: A retrospective study of 110 patients with the diagnosis of CLM at the Henan Province People's Hospital, China from August 2019 to August 2022 was performed. The clinicopathological features, relevant immunohistochemical (IHC) staining results, and fluorescence quantitative PCR of PIK3CA mutation were analyzed, and patients were followed up...
March 8, 2024: Zhonghua Bing Li Xue za Zhi Chinese Journal of Pathology
https://read.qxmd.com/read/38367239/macrophage-induced-integrin-signaling-promotes-schlemm-s-canal-formation-to-prevent-intraocular-hypertension-and-glaucomatous-optic-neuropathy
#3
JOURNAL ARTICLE
Xinyu Gu, Xun Chen, Xuan Zhang, Keli Liu, Jing-Jing Li, Wenyu Lv, Lei Zeng, Mingjuan Wu, Weibin Zhou, Weifa Wang, Shunhua Shi, Yicheng Deng, Yunhua Li, Xinbo Gao, Rong Ju, Alexandre Dubrac, Xialin Liu, Feng Zhang
Schlemm's canal (SC) functions to maintain proper intraocular pressure (IOP) by draining aqueous humor and has emerged as a promising therapeutic target for glaucoma, the second-leading cause of irreversible blindness worldwide. However, our current understanding of the mechanisms governing SC development and functionality remains limited. Here, we show that vitronectin (VTN) produced by limbal macrophages promotes SC formation and prevents intraocular hypertension by activating integrin αvβ3 signaling...
February 16, 2024: Cell Reports
https://read.qxmd.com/read/38351385/the-development-of-early-human-lymphatic-vessels-as-characterized-by-lymphatic-endothelial-markers
#4
JOURNAL ARTICLE
Shoichiro Yamaguchi, Natsuki Minamide, Hiroshi Imai, Tomoaki Ikeda, Masatoshi Watanabe, Kyoko Imanaka-Yoshida, Kazuaki Maruyama
Lymphatic vessel development studies in mice and zebrafish models have demonstrated that lymphatic endothelial cells (LECs) predominantly differentiate from venous endothelial cells via the expression of the transcription factor Prox1. However, LECs can also be generated from undifferentiated mesoderm, suggesting potential diversity in their precursor cell origins depending on the organ or anatomical location. Despite these advances, recapitulating human lymphatic malformations in animal models has been difficult, and considering lymphatic vasculature function varies widely between species, analysis of development directly in humans is needed...
February 14, 2024: EMBO Journal
https://read.qxmd.com/read/38343832/endothelial-erg-regulates-expression-of-pulmonary-lymphatic-junctional-and-inflammation-genes-in-mouse-lungs-impacting-lymphatic-transport
#5
Adri Chakraborty, Alex Kim, Salam AlAbdullatif, Joshua Campbell, Yuriy Alekseyev, Ulas Kaplan, Vrinda Dambal, Giovanni Ligresti, Maria Trojanowska
The ETS transcription factor ERG is a master regulator of endothelial gene specificity and highly enriched in the capillary, vein, and arterial endothelial cells. ERG expression is critical for endothelial barrier function, permeability, and vascular inflammation. A dysfunctional vascular endothelial ERG has been shown to impair lung capillary homeostasis, contributing to pulmonary fibrosis as previously observed in IPF lungs. Our preliminary observations indicate that lymphatic endothelial cells (LEC) in the human IPF lung also lack ERG...
January 24, 2024: Research Square
https://read.qxmd.com/read/38333673/topical-tissue-nanotransfection-of-prox1-is-effective-in-the-prophylactic-management-of-lymphedema
#6
JOURNAL ARTICLE
Ganesh Mohan, Imran Khan, Colby R Neumann, Miguel D Jorge, Shahnur Ahmed, Luci Hulsman, Mithun Sinha, Gayle M Gordillo, Chandan K Sen, Aladdin H Hassanein
Lymphedema is chronic limb swelling resulting from lymphatic dysfunction. There is no cure for the disease. Clinically, a preventive surgical approach called immediate lymphatic reconstruction (ILR) has gained traction. Experimental gene-based therapeutic approaches (e.g., using viral vectors) have had limited translational applicability. Tissue nanotransfection (TNT) technology uses a direct, transcutaneous nonviral vector, gene delivery using a chip with nanochannel poration in response to a rapid (<100 ms) focused electric field...
March 12, 2024: Molecular Therapy. Nucleic Acids
https://read.qxmd.com/read/38294731/vitamin-d-accelerates-the-subdural-hematoma-clearance-through-improving-the-meningeal-lymphatic-vessel-function
#7
JOURNAL ARTICLE
Yupeng Chen, Xuanhui Liu, Jiangyuan Yuan, Shiying Dong, Meng Nie, Weiwei Jiang, Di Wu, Mingqi Liu, Tao Liu, Chenrui Wu, Chuang Gao, Jianning Zhang, Rongcai Jiang
Subdural hematoma (SDH) drains into the extracranial lymphatic system through the meningeal lymphatic vessels (mLVs) but the formation of SDH impairs mLVs. Because vitamin D (Vit D) can protect the endothelial cells, we hypothesized that Vit D may enhance the SDH clearance. SDH was induced in Sprague-Dawley rats and treated with Vit D or vehicle. Hematoma volume in each group was measured by H&E staining and hemoglobin quantification. Evans blue (EB) quantification and red blood cells injection were used to evaluated the drainage of mLVs...
January 31, 2024: Molecular and Cellular Biochemistry
https://read.qxmd.com/read/38277016/prox1-suppresses-proliferation-and-drug-resistance-of-retinoblastoma-cells-via-targeting-notch1
#8
JOURNAL ARTICLE
Hong-Li Zhang, Na Li, Lin Dong, Hong-Xia Ma, Mo-Chi Yang
OBJECTIVE: Retinoblastoma (RB) is a prevalent type of eye cancer in youngsters. Prospero homeobox 1 (Prox1) is a homeobox transcriptional repressor and downstream target of the proneural gene that is relevant in lymphatic, hepatocyte, pancreatic, heart, lens, retinal, and cancer cells. The goal of this study was to investigate the role of Prox1 in RB cell proliferation and drug resistance, as well as to explore the underlying Notch1 mechanism. METHODS: Human RB cell lines (SO-RB50 and Y79) and a primary human retinal microvascular endothelial cell line (ACBRI-181) were used in this study...
January 26, 2024: Current Medical Science
https://read.qxmd.com/read/38273351/dysregulated-lncrnas-regulate-human-umbilical-cord-mesenchymal-stem-cell-differentiation-into-insulin-producing-cells-by-forming-a-regulatory-network-with-mrnas
#9
JOURNAL ARTICLE
Tianqin Xie, Qiming Huang, Qiulan Huang, Yanting Huang, Shuang Liu, Haixia Zeng, Jianping Liu
OBJECTIVE: In recent years, cell therapy has emerged as a new research direction in the treatment of diabetes. However, the underlying molecular mechanisms of mesenchymal stem cell (MSC) differentiation necessary to form such treatment have not been clarified. METHODS: In this study, human umbilical cord mesenchymal stem cells (HUC-MSCs) isolated from newborns were progressively induced into insulin-producing cells (IPCs) using small molecules. HUC-MSC (S0) and four induced stage (S1-S4) samples were prepared...
January 25, 2024: Stem Cell Research & Therapy
https://read.qxmd.com/read/38269589/profibrotic-vegfr3-dependent-lymphatic-vessel-growth-in-autoimmune-valvular-carditis
#10
JOURNAL ARTICLE
Victoria Osinski, Amritha Yellamilli, Maria M Firulyova, Michael J Zhang, Alyssa L Peck, Jennifer L Auger, Jessica L Faragher, Aubyn Marath, Rochus K Voeller, Timothy D O'Connell, Konstantin Zaitsev, Bryce A Binstadt
BACKGROUND: Rheumatic heart disease is the major cause of valvular heart disease in developing nations. Endothelial cells (ECs) are considered crucial contributors to rheumatic heart disease, but greater insight into their roles in disease progression is needed. METHODS: We used a Cdh5 -driven EC lineage-tracing approach to identify and track ECs in the K/B.g7 model of autoimmune valvular carditis. Single-cell RNA sequencing was used to characterize the EC populations in control and inflamed mitral valves...
January 25, 2024: Arteriosclerosis, Thrombosis, and Vascular Biology
https://read.qxmd.com/read/38244581/prox1-interaction-with-%C3%AE-sma-rich-cancer-associated-fibroblasts-facilitates-colorectal-cancer-progression-and-correlates-with-poor-clinical-outcomes-and-therapeutic-resistance
#11
JOURNAL ARTICLE
Shiue-Wei Lai, Yi-Chiao Cheng, Kee-Thai Kiu, Min-Hsuan Yen, Ying-Wei Chen, Vijesh Kumar Yadav, Chi-Tai Yeh, Kuang-Tai Kuo, Tung-Cheng Chang
BACKGROUND: The tumor microenvironment (TME) plays a vital role in tumor progression through intricate molecular interactions. Cancer-associated fibroblasts (CAFs), notably those expressing alpha-smooth muscle actin (α-SMA) or myofibroblasts, are instrumental in this context and correlate with unfavorable outcomes in colorectal cancer (CRC). While several transcription factors influence TME, the exact regulator causing CAF dysregulation in CRC remains elusive. Prospero Homeobox 1 (PROX1) stands out, as its inhibition reduces α-SMA-rich CAF activity...
January 18, 2024: Aging
https://read.qxmd.com/read/38200313/nasopharyngeal-lymphatic-plexus-is-a-hub-for-cerebrospinal-fluid-drainage
#12
JOURNAL ARTICLE
Jin-Hui Yoon, Hokyung Jin, Hae Jin Kim, Seon Pyo Hong, Myung Jin Yang, Ji Hoon Ahn, Young-Chan Kim, Jincheol Seo, Yongjeon Lee, Donald M McDonald, Michael J Davis, Gou Young Koh
Cerebrospinal fluid (CSF) in the subarachnoid space around the brain has long been known to drain through the lymphatics to cervical lymph nodes1-17 , but the connections and regulation have been challenging to identify. Here, using fluorescent CSF tracers in Prox1-GFP lymphatic reporter mice18 , we found that the nasopharyngeal lymphatic plexus is a major hub for CSF outflow to deep cervical lymph nodes. This plexus had unusual valves and short lymphangions but no smooth-muscle coverage, whereas downstream deep cervical lymphatics had typical semilunar valves, long lymphangions and smooth muscle coverage that transported CSF to the deep cervical lymph nodes...
January 10, 2024: Nature
https://read.qxmd.com/read/38193536/seizure-induced-lin28a-disrupts-pattern-separation-via-aberrant-hippocampal-neurogenesis
#13
JOURNAL ARTICLE
In-Young Choi, Jung-Ho Cha, Seong Yun Kim, Jenny Hsieh, Kyung-Ok Cho
Prolonged seizures can disrupt stem cell behavior in the adult hippocampus, an important brain structure for spatial memory. Here, using a mouse model of pilocarpine-induced status epilepticus (SE), we characterized spatiotemporal expression of Lin28a mRNA and proteins after SE. Unlike Lin28a transcripts, induction of LIN28A protein after SE was detected mainly in the subgranular zone, where immunoreactivity was found in progenitors, neuroblasts, and immature and mature granule neurons. To investigate roles of LIN28A in epilepsy, we generated Nestin-Cre:Lin28aloxP/loxP (conditional KO [cKO]) and Nestin-Cre:Lin28a+/+ (WT) mice to block LIN28A upregulation in all neuronal lineages after acute seizure...
January 9, 2024: JCI Insight
https://read.qxmd.com/read/38098084/structural-characterization-of-slym-a-4th-meningeal-membrane
#14
JOURNAL ARTICLE
Virginia Plá, Styliani Bitsika, Michael J Giannetto, Antonio Ladron-de-Guevara, Daniel Gahn-Martinez, Yuki Mori, Maiken Nedergaard, Kjeld Møllgård
Traditionally, the meninges are described as 3 distinct layers, dura, arachnoid and pia. Yet, the classification of the connective meningeal membranes surrounding the brain is based on postmortem macroscopic examination. Ultrastructural and single cell transcriptome analyses have documented that the 3 meningeal layers can be subdivided into several distinct layers based on cellular characteristics. We here re-examined the existence of a 4th meningeal membrane, Subarachnoid Lymphatic-like Membrane or SLYM in Prox1-eGFP reporter mice...
December 14, 2023: Fluids and Barriers of the CNS
https://read.qxmd.com/read/38065571/hyaluronic-acid-hydrogels-with-phototunable-supramolecular-cross-linking-for-spatially-controlled-lymphatic-tube-formation
#15
JOURNAL ARTICLE
Fei Fan, Bo Su, Alexander Kolodychak, Ephraim Ekwueme, Laura Alderfer, Sanjoy Saha, Matthew J Webber, Donny Hanjaya-Putra
The dynamics of the extracellular matrix (ECM) influences stem cell differentiation and morphogenesis into complex lymphatic networks. While dynamic hydrogels with stress relaxation properties have been developed, many require detailed chemical processing to tune viscoelasticity, offering a limited opportunity for in situ and spatiotemporal control. Here, a hyaluronic acid (HA) hydrogel is reported with viscoelasticity that is controlled and spatially tunable using UV light to direct the extent of supramolecular and covalent cross-linking interactions...
December 8, 2023: ACS Applied Materials & Interfaces
https://read.qxmd.com/read/38051669/penile-cavernous-sinusoids-are-prox1-positive-hybrid-vessels
#16
JOURNAL ARTICLE
Sarah Schnabellehner, Marle Kraft, Hans Schoofs, Henrik Ortsäter, Taija Mäkinen
Endothelial cells (ECs) of blood and lymphatic vessels have distinct identity markers that define their specialized functions. Recently, hybrid vasculatures with both blood and lymphatic vessel-specific features have been discovered in multiple tissues. Here, we identify the penile cavernous sinusoidal vessels (pc Ss) as a new hybrid vascular bed expressing key lymphatic EC identity genes Prox1, Vegfr3 and Lyve1. Using single cell transcriptome data of human corpus cavernosum tissue, we found heterogeneity within pc S endothelia and observed distinct transcriptional alterations related to inflammatory processes in hybrid ECs in erectile dysfunction associated with diabetes...
December 1, 2023: Vascular biology
https://read.qxmd.com/read/38041203/electroacupuncture-promotes-neurogenesis-in-the-dentate-gyrus-and-improves-pattern-separation-in-an-early-alzheimer-s-disease-mouse-model
#17
JOURNAL ARTICLE
Yanyi Ding, Long Li, Sinuo Wang, Yajun Cao, Minguang Yang, Yaling Dai, Huawei Lin, Jianhong Li, Yulu Liu, Zhifu Wang, Weilin Liu, Jing Tao
BACKGROUND: Impaired pattern separation occurs in the early stage of Alzheimer's disease (AD), and hippocampal dentate gyrus (DG) neurogenesis participates in pattern separation. Here, we investigated whether spatial memory discrimination impairment can be improved by promoting the hippocampal DG granule cell neogenesis-mediated pattern separation in the early stage of AD by electroacupuncture (EA). METHODS: Five familial AD mutations (5 × FAD) mice received EA treatment at Baihui and Shenting points for 4 weeks...
December 2, 2023: Biological Research
https://read.qxmd.com/read/37961461/structural-characterization-of-slym-a-4-th-meningeal-membrane
#18
Virginia Plá, Styliani Bitsika, Michael Giannetto, Antonio Ladron-de-Guevara, Daniel Gahn-Martinez, Yuki Mori, Maiken Nedergaard, Kjeld Møllgård
Traditionally, the meninges are described as 3 distinct layers, dura, arachnoid and pia. Yet, the classification of the connective meningeal membranes surrounding the brain is based on postmortem macroscopic examination. Ultrastructural and single cell transcriptome analyses have documented that the 3 meningeal layers can be subdivided into several distinct layers based on cellular characteristics. We here re-examined the existence of a 4 th meningeal membrane, S ubarachnoid Ly mphatic-like M embrane or SLYM in Prox1-eGFP reporter mice...
October 28, 2023: bioRxiv
https://read.qxmd.com/read/37961391/structural-characterization-of-slym-a-4th-meningeal-membrane
#19
Virginia Plá, Styliani Bitsika, Michael Giannetto, Antonio Ladron-de-Guevara, Daniel Gahn-Martinez, Yuki Mori, Maiken Nedergaard, Kjeld Møllgård
Traditionally, the meninges are described as 3 distinct layers, dura, arachnoid and pia. Yet, the classification of the connective meningeal membranes surrounding the brain is based on postmortem macroscopic examination. Ultrastructural and single cell transcriptome analyses have documented that the 3 meningeal layers can be subdivided into several distinct layers based on cellular characteristics. We here re-examined the existence of a 4th meningeal membrane, Subarachnoid Lymphatic-like Membrane or SLYM in Prox1-eGFP reporter mice...
October 31, 2023: Research Square
https://read.qxmd.com/read/37925421/sumoylation-triggered-alix-activation-modulates-extracellular-vesicles-circtlcd4-rwdd3-to-promote-lymphatic-metastasis-of-non-small-cell-lung-cancer
#20
JOURNAL ARTICLE
Xiayao Diao, Chao Guo, Hanhao Zheng, Ke Zhao, Yuming Luo, Mingjie An, Yan Lin, Jiancheng Chen, Yuanlong Li, Yuting Li, Xuehan Gao, Jiaqi Zhang, Mengxin Zhou, Wenliang Bai, Lei Liu, Guige Wang, Lanjun Zhang, Xiaotian He, Rusi Zhang, Zhihua Li, Changhao Chen, Shanqing Li
Lymph node (LN) metastasis is one of the predominant metastatic routes of non-small cell lung cancer (NSCLC) and is considered as a leading cause for the unsatisfactory prognosis of patients. Although lymphangiogenesis is well-recognized as a crucial process in mediating LN metastasis, the regulatory mechanism involving lymphangiogenesis and LN metastasis in NSCLC remains unclear. In this study, we employed high-throughput sequencing to identify a novel circular RNA (circRNA), circTLCD4-RWDD3, which was significantly upregulated in extracellular vesicles (EVs) from LN metastatic NSCLC and was positively associated with deteriorated OS and DFS of patients with NSCLC from multicenter clinical cohort...
November 4, 2023: Signal Transduction and Targeted Therapy
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