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Suppressor cells

Aneta A Koronowicz, Mariola Drozdowska, Barbara Wielgos, Ewelina Piasna-Słupecka, Dominik Domagała, Joanna Dulińska-Litewka, Teresa Leszczyńska
NutramilTM Complex is a multicomponent food product that meets the requirements of a food for special medical purpose. As a complete, high-energy diet it consists of properly balanced nutrients, vitamins and minerals. The aim of this study was to assess the effect of NutramilTM Complex on breast and prostate carcinoma cells. Our results showed that NutramilTM Complex reduced the viability and proliferation of breast and prostate cancer cells and that this process was associated with the induction of apoptosis via activation of caspase signalling...
2018: PloS One
P P Argyris, Z M Slama, K F Ross, A Khammanivong, M C Herzberg
Calprotectin (S100A8/A9), a heterodimeric complex of calcium-binding proteins S100A8 and S100A9, is encoded by genes mapping to the chromosomal locus 1q21.3 of the epidermal differentiation complex. Whereas extracellular calprotectin shows proinflammatory and antimicrobial properties by signaling through RAGE and TLR4, intracytoplasmic S100A8/A9 appears to be important for cellular development, maintenance, and survival. S100A8/A9 is constitutively expressed in myeloid cells and the stratified mucosal epithelia lining the oropharyngeal and genitourinary mucosae...
February 1, 2018: Journal of Dental Research
Flor M Mendez, Felipe J Núñez, Rocío I Zorrilla-Veloz, Pedro R Lowenstein, Maria G Castro
Epigenetic modifications may be involved in the development and progression of glioma. Changes in methylation and acetylation of promoters and regulatory regions of oncogenes and tumor suppressors can lead to changes in gene expression and play an important role in the pathogenesis of brain tumors. Native chromatin immunoprecipitation (ChIP) is a popular technique that allows the detection of modifications or other proteins tightly bound to DNA. In contrast to cross-linked ChIP, in native ChIP, cells are not treated with formaldehyde to covalently link protein to DNA...
January 29, 2018: Journal of Visualized Experiments: JoVE
Wei Shi, Xiaoling Wang, Lihong Ruan, Jiamei Fu, Fang Liu, Jinfeng Qu
Endometrial cancer is the most common gynecological cancer. Epithelial-mesenchymal transition (EMT) plays a critical role in tumor invasion and metastasis, which limits the success of treatment. Here, we investigated the roles of forkhead box A2 (FOXA2) and microRNA-200a (miR-200a) in regulating the EMT of endometrial cancer cells RL95-2. Empty vector or FOXA2 was stably transfected into RL95-2 cells. MTT assay measured cell proliferation, apoptosis assay measured apoptosis, Transwell invasion assay measured cell invasion, and Western blot measured the protein expression of FOXA2, E-cadherin, and vimentin...
November 1, 2017: Die Pharmazie
Shaojun Su, Xinguang Nie
Abnormal expression of miR-139 was found to be aberrantly expressed in various tumors. However, whether it is involved in osteosarcomas (OS) has never been explored. In the current study, we found that the level of ROCK1 was markedly increased in OS cancer tissues compared to that of noncancerous tissues. Meanwhile, the expression of miR-139 was markedly reduced in OS cancer tissues and cell lines. Enhanced miR-139 expression markedly suppressed colony-formation and cell invasion capacity of OS cancer cells...
December 1, 2017: Die Pharmazie
Ran Ding, Xianhua Cai, Feng Xu, Huasong Wang, Baocheng Zhang
Abnormal expression of p63 has been well identified in multiple malignancies. However, little study has been done on the association between p63 and chondro sarcoma. In the current study, we mainly explored the expression of p63 in different grades of chondrosarcoma. Our data showed that p63 was significantly decreased in grade II and III chondrosarcoma compared with that of grade I chondrosarcoma and normal control. As the characteristic of grade II and III chondrosarcoma is metastasis, we then searched the function of p63 chondrosarcoma...
July 3, 2017: Die Pharmazie
Jinjin Yuan, Zongwen Liu, Rui Song
Antisense lncRNAs play a key role in the progression of multiple cancers. Thus, it is important to elucidate the function and mechanism of antisense lncRNAs, which may play a role in the treatment of epithelial ovarian cancer (EOC). In the current study, for the first time, we showed that the level of As-SLC7A11 was markedly reduced in EOC cancer tissues and cell lines compared with those of normal control. Further study showed that silencing of As-SLC7A11 could enhance ovarian cancer cell migration. In comparison, overexpression of As-SLC7A11 markedly induced ovarian cancer cell apoptosis...
July 3, 2017: Die Pharmazie
Jingyuan Wang, Xuemei Wang, Fengjun Liu, Yuguang Fu
Colorectal cancer is one of the most commonly diagnosed cancers. Recently, several microRNAs (miRNAs) have been characterized as oncogenes or tumor suppressors in colorectal cancer. This study was aimed to explore the tumor suppressive effects and its underling mechanism of miR-335 on colorectal cancer cells. Human colorectal cancer HCT116 cells were employed and the expression of miR-335 in cells was altered by transfection with miR-335 mimic and miR-335 inhibitor. Thereafter, CCK-8 assay, flow cytometry, Transwell assay and Western blotting were used to detect cell viability, apoptosis, migration, invasion, and the expression of epithelial-mesenchymal transition (EMT)-related proteins...
August 1, 2017: Die Pharmazie
Haijun Liu, Xiuhua Xie, Xiuqin Yang, Yanguo Li, Ying Wang, Dongmei Xu
MiR-136 acts as a tumor suppressor by promoting cell apoptosis and downregulating Bcl-2 in glioma cells. Hence, an attempt has been made to evaluate the role of miR-136 in regulation of inflammatory damage in HK-2 cells. HK-2 cells were cultured and assessed for viability. The cells were then transfected with miR-136 mimic, si- miR-136, si-Klotho, and NC. Dual luciferase test was performed to confirm the target of miR-136 which was assumed to be Klotho. Cell viability, apoptosis, expressions of inflammatory cytokines like TNF-α, IL-1β, IL-6 and IL-8 were assessed in HK-2 cells with overexpressing miR-136 or with knocked down miR-136 activities, following exposure to LPS...
May 1, 2017: Die Pharmazie
Yong Lin, Qian Chen, Quan-Xing Liu, Dong Zhou, Xiao Lu, Xu-Feng Deng, Hua Yang, Hong Zheng, Yuan Qiu
Cancer cell invasion and unlimited proliferation are key factors in patients with colorectal cancer (CRC). Increased protein deglycase DJ-1 in cancer cells is known to promote tumor growth; however, its role in CRC progression is not well defined. In this study, we investigated 100 CRC patients with disease stages I-IV to determine whether DJ-1 could serve as a prognostic biomarker in CRC. These results showed that DJ-1 expression in CRC tissues was higher than that in normal colon tissues and was associated with the (Tumor Node Metastasis) TNM stage...
February 14, 2018: Cancer Medicine
Weijiang Zhang, Jing Fei, Shuqian Yu, Jiayu Shen, Xiaoqing Zhu, Annapurna Sadhukhan, Weiguo Lu, Jianwei Zhou
The roles of long non-coding RNAs (lncRNAs), a class of long non-protein-coding RNAs, in the tumorigenesis of ovarian epithelial cells remain unknown. In this study, we discovered that the expression of long intergenic non-coding RNA 1088 (LINC01088) was clearly reduced in benign epithelial ovarian tumor tissues compared to matched normal ovarian tissues. This was shown by global cDNA gene chip scanning and real-time qPCR, and validated in 42 clinical specimens. Furthermore, we found that LINC01088 inhibited the growth of ovarian cancer xenografts in nude mice...
February 13, 2018: Scientific Reports
Ron J Okagaki, Allison Haaning, Hatice Bilgic, Shane Heinen, Arnis Druka, Micha Bayer, Robbie Waugh, Gary J Muehlbauer
The shoot apical and axillary meristems control shoot development, effectively influencing lateral branch and leaf formation. The barley (Hordeum vulgare L.) uniculm2 (cul2) mutation blocks axillary meristem development and mutant plants lack lateral branches (tillers) that normally develop from the crown. A genetic screen for cul2 suppressors recovered two recessive alleles of ELIGULUM-A (ELI-A) that partially rescued the cul2 tillering phenotype. Mutations in ELI-A produce shorter plants with fewer tillers and disrupt the leaf blade-sheath boundary, producing liguleless leaves and reduced secondary cell wall development in stems and leaves...
February 12, 2018: Plant Physiology
Pritha Dasgupta, Priyanka Kulkarni, Shahana Majid, Shahryari Varahram, Yutaka Hashimoto, Nadeem S Bhat, Marisa Shiina, Guoren Deng, Sharanjot Saini, Z Laura Tabatabai, Soichiro Yamamura, Yuichiro Tanaka, Rajvir Dahiya
This study aims to investigate the role of miR-203-HOTAIR interaction in the suppression of renal cell carcinoma (RCC). We employed series of in vitro assays such as proliferation, invasion, migration and colony formation along with in vivo tumor xenograft model. Profiling of miR-203 and HOTAIR expression revealed that miR-203 was significantly under-expressed whereas HOTAIR was overexpressed in RCC cell-lines and clinical specimens compared to normal cell line and tissue. Both miR-203 and HOTAIR expression significantly distinguished malignant from normal tissues and significantly correlated with clinicopathological characteristics of patients...
February 13, 2018: Molecular Cancer Therapeutics
Pavlos Msaouel, Nizar M Tannir, Cheryl Lyn Walker
Renal medullary carcinoma (RMC) is a highly aggressive malignancy that predominantly afflicts young adults and adolescents with sickle hemoglobinopathies. It is characterized by complete loss of expression of the chromatin remodeler and tumor suppressor SMARCB1. Despite therapy, the outcomes of patients with RMC remain very poor, highlighting the need to understand the etiology of this cancer, and develop new diagnostic, preventive, and therapeutic strategies. A key knowledge gap in RMC biology is why sickle hemoglobinopathies predispose to the development of this cancer...
February 12, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Marten Hornsveld, Lydia M Smits, Maaike Meerlo, Miranda van Amersfoort, Marian J Groot Koerkamp, Dik van Leenen, David E Kloet, Frank C Holstege, Patrick W B Derksen, Boudewijn M T Burgering, Tobias B Dansen
FOXO transcription factors are regulators of cellular homeostasis and putative tumor suppressors, yet the role of FOXO in cancer progression remains to be determined. The data on FOXO function, particularly for epithelial cancers, are fragmentary and come from studies that focused on isolated aspects of cancer. To clarify the role of FOXO in epithelial cancer progression, we characterized the effects of inducible FOXO activation and loss in a mouse model of metastatic invasive lobular carcinoma. Strikingly, either activation or loss of FOXO function suppressed tumor growth and metastasis...
February 12, 2018: Cancer Research
Sarmishtha De, Daniel J Lindner, Claire Coleman, Gary Wildey, Afshin Dowlati, George R Stark
Traditional treatments of small cell lung cancer (SCLC) with cisplatin, a standard of care therapy, spare the tumor-initiating cells (TIC) that mediate drug resistance. Here we report a novel therapeutic strategy that preferentially targets TIC in SCLC in which cisplatin is combined with CBL0137, an inhibitor of the histone chaperone facilitates chromatin transcription (FACT), which is highly expressed in TIC. Combination of cisplatin and CBL0137 killed patient-derived and murine SCLC cell lines synergistically...
February 13, 2018: Cancer Research
Xingwang Xie, Xueyan Wang, Weijia Liao, Ran Fei, Nan Wu, Xu Cong, Qian Chen, Lai Wei, Yu Wang, Hongsong Chen
BACKGROUND: Identification of novel MDM2 or p53 binding proteins may reveal undefined oncogenes, tumor suppressors, signaling pathways and possible treatment targets. METHODS: By means of immunoprecipitation and Mass Spectrometry analysis, we aimed to identify novel regulators of the MDM2-p53 pathway. We further clarified the impact of MYL6B on the p53 protein level and on the process of apoptosis. We also investigated the role of MYL6B in hepatocellular carcinoma by clone formation assay and by determining the correlation between its expression and prognosis of HCC patients...
February 13, 2018: Journal of Experimental & Clinical Cancer Research: CR
Shengyun Cai, Pei Zhang, Suhe Dong, Li Li, Jianming Cai, Mingjuan Xu
BACKGROUND/AIMS: Ovarian cancer (OC) is the fifth leading cause of cancer-related death in women, and it is difficult to diagnose at an early stage. The purpose of this study was to explore the prognostic biological markers of OC. METHODS: Univariate Cox regression analysis was used to identify genes related to OC prognosis from the Cancer Genome Atlas(TCGA) database. Immunohistochemistry was used to analyse the level of SPINK13 in OC and normal tissues. Cell proliferation, apoptosis and invasion were performed using MTT assay, flow cytometric analysis and Transwell assay, respectively...
February 7, 2018: Cellular Physiology and Biochemistry
Wei Jiang, Xiaoyan Zhou, Zengxia Li, Kaiyu Liu, Weige Wang, Renke Tan, Xiaoji Cong, Jiaoyu Shan, Yanxia Zhan, Zhaomeng Cui, Lizhi Jiang, Quanfu Li, Suqin Shen, Meirong Bai, Yunfeng Cheng, Bin Li, Minjia Tan, Dengke K Ma, Jun O Liu, Yongjun Dang
B-cell lymphomas are heterogeneous blood disorders with limited therapeutic options, largely because of its propensity to relapse and become refractory to treatments. Carabin, a key suppressor of B cell receptor signaling and proliferation, is inactivated in B-cell lymphoma by unknown mechanisms. Here we identify P4HA2 (Prolyl 4-Hydroxylase 2) as a specific proline hydroxylase of Carabin. Carabin hydroxylation leads to its proteasomal degradation, thereby activating the Ras/ERK pathway and increasing B-cell lymphoma proliferation...
February 1, 2018: Blood
Ling Zhou, Kun Miao, Bingjiao Yin, Huaping Li, Jiahui Fan, Yazhen Zhu, Hongping Ba, Zunyue Zhang, Fang Chen, Jing Wang, Chunxia Zhao, Zhuoya Li, Dao Wen Wang
Background -Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population of cells that expand in cancer, inflammation, and infection and negatively regulate inflammation and the immune response. Heart failure (HF) is a complex clinical syndrome, wherein inflammation induction and incomplete resolution can potentially contribute to HF development and progression. However, the role of MDSCs in HF remains unclear. Methods -The percentage of MDSCs in HF patients and in mice with pressure overload-induced HF using isoproterenol (ISO) infusion or transverse aortic constriction (TAC), was detected by flow cytometry...
February 1, 2018: Circulation
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