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carbonic anhydrase

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https://www.readbyqxmd.com/read/28429509/co2-permeability-and-carbonic-anhydrase-activity-of-rat-cardiomyocytes
#1
Mariela Arias-Hidalgo, Samer Al-Samir, Natalie Weber, Cornelia Geers-Knörr, Gerolf Gros, Volker Endeward
AIM: We determine here the CO2 permeability (PCO2 ) of plasma membranes of cardiomyocytes. These cells were chosen because heart possesses the highest rate of O2 -consumption/CO2 -production in the body. METHODS: Cardiomyocytes were isolated from rat hearts using the Langendorff technique. Cardiomyocyte suspensions exhibited a vitality of 2-14% and were studied by the previously described mass spectrometric (18) O-exchange-technique deriving PCO2 . We show by mass spectrometry and by carbonic anhydrase (CA)-staining that non-vital cardiomyocytes are free of CA and thus do not contribute to the mass spectrometric signal, which is determined exclusively by the fully functional vital cardiomyocytes...
April 20, 2017: Acta Physiologica
https://www.readbyqxmd.com/read/28428780/genomic-and-transcriptomic-insights-into-calcium-carbonate-biomineralization-by-marine-actinobacterium-brevibacterium-linens-bs258
#2
Yuying Zhu, Ning Ma, Weihua Jin, Shimei Wu, Chaomin Sun
Calcium carbonate (CaCO3) biomineralization has been investigated due to its wide range of scientific and technological implications, however, the molecular mechanisms of this important geomicrobiological process are largely unknown. Here, a urease-positive marine actinobacterium Brevibacterium linens BS258 was demonstrated to effectively form CaCO3 precipitates. Surprisingly, this bacterium could also dissolve the formed CaCO3 with the increase of the Ca(2+) concentration. To disclose the mechanisms of biomineralization, the genome of B...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28428618/pulmonary-delivery-of-triptolide-loaded-liposomes-decorated-with-anti-carbonic-anhydrase-ix-antibody-for-lung-cancer-therapy
#3
Congcong Lin, Blenda Chi Kwan Wong, Hubiao Chen, Zhaoxiang Bian, Ge Zhang, Xue Zhang, Muhammad Kashif Riaz, Deependra Tyagi, Ge Lin, Yanbo Zhang, Jinjin Wang, Aiping Lu, Zhijun Yang
Antibody-decorated liposomes can facilitate the precise delivery of chemotherapeutic drugs to the lung by targeting a recognition factor present on the surface of lung tumor cells. Carbonic anhydrase IX (CA IX) is an enzyme expressed on the surface of lung cancer cells with a restricted expression in normal lungs. Here, we explored the utility of anti-carbonic anhydrase IX (CA IX) antibody, conjugated to the surface of triptolide (TPL)-loaded liposomes (CA IX-TPL-Lips), to promote the therapeutic effects for lung cancer via pulmonary administration...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28423504/variations-in-the-bitterness-perception-related-genes-tas2r38-and-ca6-modify-the-risk-for-colorectal-cancer-in-koreans
#4
Jeong-Hwa Choi, Jeonghee Lee, Jae Hwan Oh, Hee Jin Chang, Dae Kyung Sohn, Aesun Shin, Jeongseon Kim
Bitterness perception is known to be an important factor in individuals' dietary behaviors and is also associated with the sensing of nutritious/noxious molecules for subsequent metabolic responses in multiple organs. Therefore, the genetic variation in bitterness sensing may be associated with diet-related diseases, including colorectal cancer (CRC). We investigated the influence of variations in the bitterness-sensing genes taste receptor type 2 member 38 (TAS2R38) and carbonic anhydrase 6 (CA6) on the consumption of food, tobacco and alcohol and the risk of CRC in Koreans...
March 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28420165/impact-of-acetazolamide-a-carbonic-anhydrase-inhibitor-on-the-development-of-intestinal-polyps-in-min-mice
#5
Nobuharu Noma, Gen Fujii, Shingo Miyamoto, Masami Komiya, Ruri Nakanishi, Misato Shimura, Sei-Ichi Tanuma, Michihiro Mutoh
Colorectal cancer is a common cancer worldwide. Carbonic anhydrase (CA) catalyzes the reversible conversion of carbon dioxide to bicarbonate ion and a proton, and its inhibitor is reported to reduce cancer cell proliferation and induce apoptosis. Therefore, we asked whether acetazolamide, a CA inhibitor, could inhibit intestinal carcinogenesis. Five-week-old male Apc-mutant mice, Min mice, were fed a AIN-76A diet containing 200 or 400 ppm acetazolamide. As a result, acetazolamide treatment reduced the total number of intestinal polyps by up to 50% compared to the control group...
April 17, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28416105/do-clear-cell-papillary-renal-cell-carcinomas-have-malignant-potential-diolombi-ml-cheng-l-argani-p-epstein-ji-am-j-surg-pathol-december-2015-39-12-1621-1634
#6
Oleksandr N Kryvenko
There have been no recurrences or metastases of clear cell papillary renal cell carcinoma (CCPRCC) in 268 reported cases with follow-up in the English-language literature. We identified all our cases of CCPRCC (1990-2013), reviewing all cases that preceded the formal designation of the entity. Immunohistochemical stains were performed on 32 cases during their initial workup. In addition, stains for carbonic anhydrase IX and cytokeratin 7 were performed on 2 cases, one with atypical follow-up and the other with a more compact morphology, although not performed initially...
April 13, 2017: Urologic Oncology
https://www.readbyqxmd.com/read/28416101/n-substituted-and-ring-opened-saccharin-derivatives-selectively-inhibit-transmembrane-tumor-associated-carbonic-anhydrases-ix-and-xii
#7
Jekaterīna Ivanova, Fabrizio Carta, Daniela Vullo, Janis Leitans, Andris Kazaks, Kaspars Tars, Raivis Žalubovskis, Claudiu T Supuran
A series of N-substituted saccharins incorporating aryl, alkyl and alkynyl moieties, as well as some ring opened derivatives were prepared and investigated as inhibitors of the metalloenzyme carbonic anhydrase (CA, EC 4.2.1.1). The widespread cytosolic isoforms CA I and II were not inhibited by these sulfonamides whereas transmembrane, tumor-associated ones were effectively inhibited, with KIs in the range of 22.1-481nM for CA IX and of 3.9-245nM for hCA XII. Although the inhibition mechanism of these tertiary/secondary sulfonamides is unknown for the moment, the good efficacy and especially selectivity for the inhibition of the tumor-associated over the cytosolic, widespread isoforms, make these derivatives of considerable interest as enzyme inhibitors with various pharmacologic applications...
April 7, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28414463/do-fragments-and-crystallization-additives-bind-similarly-to-drug-like-ligands
#8
Malgorzata N Drwal, Célien Jacquemard, Carlos Perez, Jérémy Desaphy, Esther Kellenberger
The success of fragment-based drug design (FBDD) hinges upon the optimization of low molecular weight compounds (MW<300) with weak binding affinities to lead compounds with high affinity and selectivity. Usually, structural information from fragment-protein complexes is used to develop ideas about the binding mode of similar, but drug-like molecules. In this regard, crystallization additives such as cryoprotectants or buffer components, highly abundant in crystal structures, are frequently ignored. Thus, the aim of the study was the investigation of the information present in both protein complexes with fragments as well as additives and how they relate to the binding modes of their drug-like counterparts...
April 17, 2017: Journal of Chemical Information and Modeling
https://www.readbyqxmd.com/read/28414240/light-activated-cos-h2s-donation-from-photocaged-thiocarbamates
#9
Yu Zhao, Sarah G Bolton, Michael D Pluth
Hydrogen sulfide (H2S) is an important biomolecule, and responsive chemical tools for its delivery are needed. Here, we utilize the photocleavable o-nitrobenzyl group to unmask caged thiocarbamates and to access photoactivated H2S releasing molecules. These donors function by the initial release of carbonyl sulfide (COS), which is quickly hydrolyzed to H2S by carbonic anhydrase (CA). Our investigations demonstrate that o-nitrobenzyl-caged thiocarbamates can serve as a donor platform for the bio-orthogonal stimulated release of COS/H2S...
April 17, 2017: Organic Letters
https://www.readbyqxmd.com/read/28413868/goniosynechialysis-and-repositioning-of-intraocular-lens-in-a-case-of-secondary-angle-closure-glaucoma
#10
Masaru Fujita, Masafumi Ogata, Yasuyuki Suzuki, Tsunetomo Ichihashi, Miyuki Nagahara, Kenji Kawai
PURPOSE: Implanting intraocular lens (IOL) in proper direction is important in cataract surgery to reduce the possibility of undesirable complications. We experienced a case who underwent vitrectomy combined with cataract surgery and developed secondary angle closure glaucoma caused by IOL misdirection. Goniosynechialysis (GSL) and repositioning of IOL successfully ameliorated the high intraocular pressure (IOP). CASE REPORT: A 64-year-old male with massive vitreous hemorrhage underwent vitrectomy combined with cataract surgery...
April 20, 2017: Tokai Journal of Experimental and Clinical Medicine
https://www.readbyqxmd.com/read/28413723/physiological-effects-of-five-different-marine-natural-organic-matters-noms-and-three-different-metals-cu-pb-zn-on-early-life-stages-of-the-blue-mussel-mytilus-galloprovincialis
#11
Lygia Sega Nogueira, Adalto Bianchini, Scott Smith, Marianna Basso Jorge, Rachael L Diamond, Chris M Wood
Metals are present in aquatic environments as a result of natural and anthropogenic inputs, and may induce toxicity to organisms. One of the main factors that influence this toxicity in fresh water is natural organic matter (NOM) but all NOMs are not the same in this regard. In sea water, possible protection by marine NOMs is not well understood. Thus, our study isolated marine NOMs by solid-phase extraction from five different sites and characterized them by excitation-emission fluorescence analysis-one inshore (terrigenous origin), two offshore (autochthonous origin), and two intermediate in composition (indicative of a mixed origin)...
2017: PeerJ
https://www.readbyqxmd.com/read/28410215/cardiac-glycosides-suppress-the-maintenance-of-stemness-and-malignancy-via-inhibiting-hif-1%C3%AE-in-human-glioma-stem-cells
#12
Dae-Hee Lee, Sang Cheul Oh, Amber J Giles, Jinkyu Jung, Mark R Gilbert, Deric M Park
Tissue hypoxia contributes to solid tumor pathogenesis by activating a series of adaptive programs. We previously showed that hypoxia promotes the preferential expansion and maintenance of CD133 positive human glioma stem cells (GSC) in a hypoxia inducible factor 1 alpha (HIF-1α)-dependent mechanism. Here, we examined the activity of digitoxin (DT), a cardiac glycoside and a putative inhibitor of HIF-1α, on human GSC in vitro and in vivo. During hypoxic conditions (1% O2), we observed the effect of DT on the intracellular level of HIF-1α and the extracellular level of vascular endothelial growth factor (VEGF) in human GSC...
March 30, 2017: Oncotarget
https://www.readbyqxmd.com/read/28406140/spicule-formation-in-calcareous-sponges-coordinated-expression-of-biomineralization-genes-and-spicule-type-specific-genes
#13
Oliver Voigt, Maja Adamska, Marcin Adamski, André Kittelmann, Lukardis Wencker, Gert Wörheide
The ability to form mineral structures under biological control is widespread among animals. In several species, specific proteins have been shown to be involved in biomineralization, but it is uncertain how they influence the shape of the growing biomineral and the resulting skeleton. Calcareous sponges are the only sponges that form calcitic spicules, which, based on the number of rays (actines) are distinguished in diactines, triactines and tetractines. Each actine is formed by only two cells, called sclerocytes...
April 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28405482/the-relationship-between-serum-carbonic-anhydrase-i-ii-autoantibody-levels-and-diabetic-retinopathy-in-type-1-diabetes-patients
#14
Adem Türk, Süleyman Mollamehmetoğlu, Ahmet Alver, Ahmet Menteşe, İrfan Nuhoğlu, Cihangir Erem, Halil İbrahim İmamoğlu
OBJECTIVES: To investigate the relationship between serum carbonic anhydrase I-II (CA-I and II) autoantibody levels and diabetic retinopathy (DRP) in cases with type 1 diabetes. MATERIALS AND METHODS: A total of 37 type-1 diabetic patients, 17 with DRP (group 1) and 20 without (group 2), and 38 healthy control subjects (group 3) were included. CA-I and CA-II autoantibody levels were measured in serum samples obtained from each of the three groups and compared statistically...
April 2017: Turkish Journal of Ophthalmology
https://www.readbyqxmd.com/read/28402608/a-fast-algorithm-to-compute-conical-pockets-in-proteins-application-to-the-structural-characterization-of-%C3%AE-carbonic-anhydrases
#15
Michel Petitjean
Some major proteins families, such as carbonic anhydrases (CAs), have a conical cavity at the active site. No algorithm was available to compute conical cavities, so we needed to design one. The fast algorithm we designed let us show on a set of 717 CAs extracted from the PDB database that γ-CAs are characterized by active site cavity cone angles significantly larger than those of α-CAs and β-CAs: the generatrix-axis angles are greater than 60° for the γ-CAs while they are smaller than 50° for the other CAs...
April 12, 2017: Molecular Informatics
https://www.readbyqxmd.com/read/28400867/evaluation-of-autoantibodies-in-patients-with-primary-and-secondary-sjogren-s-syndrome
#16
Ellen De Langhe, Xavier Bossuyt, Long Shen, Kishore Malyavantham, Julian L Ambrus, Lakshmanan Suresh
BACKGROUND: Antibodies to salivary gland protein 1 (SP1), carbonic anhydrase 6 (CA6) and parotid secretory protein (PSP) were discovered in an animal model of Sjogren's syndrome (SS). Their expression was noted in patients with SS, especially those with lower focus scores on lip biopsies and those with early disease lacking antibodies to Ro and La. OBJECTIVE: The current studies evaluated these autoantibodies in patients with long-standing SS expressing high levels of anti-Ro antibodies and in patients with Sjogren's syndrome secondary to systemic lupus erythematosus (SLE), systemic sclerosis (SSc) and mixed connective tissue disease (MCTD)...
2017: Open Rheumatology Journal
https://www.readbyqxmd.com/read/28400735/a-novel-stopped-flow-assay-for-quantitating-carbonic-anhydrase-activity-and-assessing-red-blood-cell-hemolysis
#17
Pan Zhao, R Ryan Geyer, Walter F Boron
We report a novel carbonic-anhydrase (CA) assay and its use for quantitating red-blood-cell (RBC) lysis during stopped-flow (SF) experiments. We combine two saline solutions, one containing HEPES/pH 7.03 and the other, ~1% CO2/44 mM [Formula: see text]/pH 8.41, to generate an out-of-equilibrium CO2/[Formula: see text] solution containing ~0.5% CO2/22 [Formula: see text]/pH ~7.25 (10°C) in the SF reaction cell. CA catalyzes relaxation of extracellular pH to ~7.50: [Formula: see text] + H(+) → CO2 + H2O. Proof-of-concept studies (no intact RBCs) show that the pH-relaxation rate constant (kΔpH)-measured via pyranine fluorescence-rises linearly with increases in [bovine CAII] or [murine-RBC lysate]...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28400084/synthesis-and-biological-evaluation-of-novel-aromatic-and-heterocyclic-bis-sulfonamide-schiff-bases-as-carbonic-anhydrase-i-ii-vii-and-ix-inhibitors
#18
Suleyman Akocak, Nabih Lolak, Alessio Nocentini, Gulcin Karakoc, Anzel Tufan, Claudiu T Supuran
A series of sixteen novel aromatic and heterocyclic bis-sulfonamide Schiff bases were prepared by conjugation of well known aromatic and heterocyclic aminosulfonamide carbonic anhydrase (CA, EC 4.2.1.1) inhibitor pharmacophores with aromatic and heterocyclic bis-aldehydes. The obtained bis-sulfonamide Schiff bases were investigated as inhibitors of four selected human (h) CA isoforms, hCA I, hCA II, hCA VII and hCA IX. Most of the newly synthesized compounds showed a good inhibitory profile against isoforms hCA II and hCA IX, also showing moderate selectivity against hCA I and VII...
March 31, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28398591/evolutionarily-distinct-strategies-for-the-acquisition-of-inorganic-carbon-from-seawater-in-marine-diatoms
#19
Yoshinori Tsuji, Anggara Mahardika, Yusuke Matsuda
The acquisition of dissolved inorganic carbon (DIC) in CO2-limited seawater is a central issue to understand in marine primary production. We previously demonstrated the occurrence of direct HCO3- uptake by solute carrier (SLC) 4 transporters in a diatom, a major marine primary producer. Homologs of SLC are found in both centric and pennate marine diatoms, suggesting that SLC transporters are generally conserved. Here, the generality of SLC-mediated DIC uptake in diatoms was examined using an SLC inhibitor, diisothiocyano-2,2'-stilbenedisulfonic acid (DIDS), and an inhibitor of external carbonic anhydrase, acetazolamide...
April 8, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28396658/characterisation-of-aerotolerant-forms-of-a-robust-chicken-colonizing-campylobacter-coli
#20
Peter M O'Kane, Ian F Connerton
Campylobacter contaminated poultry meat is a major source of human foodborne illness. Campylobacter coli strain OR12 is a robust colonizer of chickens that was previously shown to outcompete and displace other Campylobacter strains from the chicken's gastrointestinal tract. This strain is capable of aerobic growth on blood agar. Serial aerobic passage increased this aerotolerance as assessed by quantitative assays for growth and survival on solid media. Aerotolerance was also associated with increased peroxide stress resistance...
2017: Frontiers in Microbiology
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