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carbonic anhydrase

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https://www.readbyqxmd.com/read/28238511/comparison-of-the-anion-inhibition-profiles-of-the-%C3%AE-and-%C3%AE-carbonic-anhydrases-from-the-pathogenic-bacterium-burkholderia-pseudomallei
#1
Sonia Del Prete, Daniela Vullo, Pietro di Fonzo, Vincenzo Carginale, Claudiu T Supuran, Clemente Capasso
We report the cloning, purification and characterization of BpsβCA, a β-class carbonic anhydrase (CA, EC 4.2.1.1) from the pathogenic bacterium Burkholderia pseudomallei, the etiological agent of melioidosis, and compare its activity and inhibition with those of the γ-CA from the same organism, BpsγCA, recently investigated by our groups. BpsβCA showed a significant catalytic activity for the physiologic, CO2 hydration reaction, with the following kinetic parameters, kcat of 1.6×10(5)s(-1) and kcat/Km of 3...
February 16, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28238040/biocalcite-and-carbonic-acid-activators
#2
Xiaohong Wang, Meik Neufurth, Emad Tolba, Shunfeng Wang, Heinz C Schröder, Werner E G Müller
Based on evolution of biomineralizing systems and energetic considerations, there is now compelling evidence that enzymes play a driving role in the formation of the inorganic skeletons from the simplest animals, the sponges, up to humans. Focusing on skeletons based on calcium minerals, the principle enzymes involved are the carbonic anhydrase (formation of the calcium carbonate-based skeletons of many invertebrates like the calcareous sponges, as well as deposition of the calcium carbonate bioseeds during human bone formation) and the alkaline phosphatase (providing the phosphate for bone calcium phosphate-hydroxyapatite formation)...
2017: Progress in Molecular and Subcellular Biology
https://www.readbyqxmd.com/read/28237553/human-carbonic-anhydrase-inhibitory-profile-of-mono-and-bis-sulfonamides-synthesized-via-a-direct-sulfochlorination-of-3-and-4-hetero-arylisoxazol-5-amine-scaffolds
#3
Mikhail Krasavin, Mikhail Korsakov, Zhanna Zvonaryova, Evgenii Semyonychev, Tiziano Tuccinardi, Stanislav Kalinin, Muhammet Tanç, Claudiu T Supuran
Three distinct series of isoxazole-based primary mono- and bis-sulfonamides have been synthesized via direct sulfochlorination, each of them delivering nanomolar inhibitors of human carbonic anhydrase. Certain pronounced SAR trends have been established and rationalized by in silico docking. These findings expand the structure-activity knowledge base for heterocycle-containing sulfonamide carbonic anhydrase inhibitors and further validate the power of direct electrophilic sulfochlorination as a means of introducing the pharmacophoric primary sulfonamide group into structurally diverse aromatic precursors...
February 13, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28235409/acetazolamide-potentiates-the-anti-tumor-potential-of-hdaci-ms-275-in-neuroblastoma
#4
Reza Bayat Mokhtari, Narges Baluch, Micky Ka Hon Tsui, Sushil Kumar, Tina S Homayouni, Karen Aitken, Bikul Das, Sylvain Baruchel, Herman Yeger
BACKGROUND: Neuroblastoma (NB), a tumor of the primitive neural crest, despite aggressive treatment portends a poor long-term survival for patients with advanced high stage NB. New treatment strategies are required. METHODS: We investigated coordinated targeting of essential homeostatic regulatory factors involved in cancer progression, histone deacetylases (HDACs) and carbonic anhydrases (CAs). RESULTS: We evaluated the antitumor potential of the HDAC inhibitor (HDACi), pyridylmethyl-N-{4-[(2-aminophenyl)-carbamoyl]-benzyl}-carbamate (MS-275) in combination with a pan CA inhibitor, acetazolamide (AZ) on NB SH-SY5Y, SK-N-SH and SK-N-BE(2) cells...
February 24, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28230813/characterization-of-carbonic-anhydrase-9-in-the-alimentary-canal-of-aedes-aegypti-and-its-relationship-to-homologous-mosquito-carbonic-anhydrases
#5
Daniel P Dixon, Leslie Van Ekeris, Paul J Linser
In the mosquito midgut, luminal pH regulation and cellular ion transport processes are important for the digestion of food and maintenance of cellular homeostasis. pH regulation in the mosquito gut is affected by the vectorial movement of the principal ions including bicarbonate/carbonate and protons. As in all metazoans, mosquitoes employ the product of aerobic metabolism carbon dioxide in its bicarbonate/carbonate form as one of the major buffers of cellular and extracellular pH. The conversion of metabolic carbon dioxide to bicarbonate/carbonate is accomplished by a family of enzymes encoded by the carbonic anhydrase gene family...
February 21, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28229634/biochemical-characterization-of-the-native-%C3%AE-carbonic-anhydrase-purified-from-the-mantle-of-the-mediterranean-mussel-mytilus-galloprovincialis
#6
Rosa Perfetto, Sonia Del Prete, Daniela Vullo, Giovanni Sansone, Carmela Barone, Mosè Rossi, Claudiu T Supuran, Clemente Capasso
A α-carbonic anhydrase (CA, EC 4.2.1.1) has been purified and characterized biochemically from the mollusk Mytilus galloprovincialis. As in most mollusks, this α-CA is involved in the biomineralization processes leading to the precipitation of calcium carbonate in the mussel shell. The new enzyme had a molecular weight of 50 kDa, which is roughly two times higher than that of a monomeric α-class enzyme. Thus, Mytilus galloprovincialis α-CA is either a dimer, or similar to the Tridacna gigas CA described earlier, may have two different CA domains in its polypeptide chain...
December 2017: Journal of Enzyme Inhibition and Medicinal Chemistry
https://www.readbyqxmd.com/read/28225196/effective-access-to-multivalent-inhibitors-of-carbonic-anhydrases-promoted-by-peptide-bioconjugation
#7
Nasreddine Kanfar, Muhammet Tanc, Pascal Dumy, Claudiu Supuran, Sébastien Ulrich, Jean-Yves Winum
Multivalency has impressive effects on (bio)molecular recognition, through the simultaneous presentation of multiple copies of a ligand, which can change a weak millimolar binder into a potent nanomolar one. The implementation of multivalency in enzyme inhibition is rather recent, being exemplified by few serendipitous discoveries, and hitherto relying on the random exploration of new multivalent structures as potential enzyme inhibitors. We report here a straightforward and versatile method that enables the construction of multivalent systems for the inhibition of carbonic anhydrases (CA), widespread enzymes that catalyse a fundamental biochemical reaction...
February 22, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28220172/mechanistic-insights-into-biomimetic-carbonic-anhydrase-action-catalyzed-by-doped-carbon-nanotubes-and-graphene
#8
Manju Verma, Parag A Deshpande
Electronic structural analyses of hydrogen terminated metal doped carbon nanotube/graphene (M-CNT/Gr, MN3-CNT/Gr, M = Ru/Rh) and ruthenium cluster decorated carbon nanotube/graphene (Ru4-CNT/Gr) were carried out for examining the biomimetic catalytic activity towards CO2 hydration reaction. The carbonic anhydrase action was followed for the reaction of CO2 with H2O resulting in a bicarbonate ion and a proton. All the catalysts were found to be active for CO2 hydration and the mechanism proved them to be biomimetic...
February 21, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28217890/cerebellar-hemangioblastoma-with-perivascular-pseudorosette-formation-and-glial-differentiation-a-case-report
#9
Jian Wang, Xu-Yong Lin, Xue-Shan Qiu, En-Hua Wang, Lian-He Yang, Juan-Han Yu
Hemangioblastoma is a well-circumscribed, highly vascular, lipid-rich and low-grade tumor of uncertain histogenesis. Its histopathological features have been well established. Herein, we present a case of cerebellar hemangioblastoma in a 43-year-old woman. Histologically, the tumor was predominantly composed of cellular areas showing eosinophilic or vacuolated stromal cells arranged in nests and sheets. Focally, conventional reticular areas could be seen. Additionally, in some areas, the stromal cells were arranged radially around blood vessels, exhibiting perivascular pseudorosette structures, which were similar mostly to those of ependymomas...
August 24, 2016: Neuropathology: Official Journal of the Japanese Society of Neuropathology
https://www.readbyqxmd.com/read/28217551/cost-effectiveness-of-glaucoma-management-with-monotherapy-medications-in-egypt
#10
Amal Abd-Elaal El-Khamery, Amir Ibrahim Mohamed, Hassan Eisa Hassan Swify, Alaa Ibrahim Mohamed
Glaucoma is a serious chronic ophthalmic disease since it causes irreversible visual disability if untreated can lead to blindness. Treatment options include medications (classified into five major classes of drugs which are muscarinic cholinergic agonists, alpha-2 adrenergic agonists, beta-1 adrenergic antagonists, prostaglandins [PGs], and carbonic anhydrase inhibitors); use of laser therapy or conventional surgery. Pharmacoeconomic analysis helps in choosing among this variety of treatments. There is a great need for such analysis in Egypt since undergoing of it in different countries or societies may produce different results...
January 2017: Journal of Advanced Pharmaceutical Technology & Research
https://www.readbyqxmd.com/read/28212997/differential-expression-and-prognostic-significance-of-glut1-according-to-histologic-type-of-non-small-cell-lung-cancer-and-its-association-with-volume-dependent-parameters
#11
Young Wha Koh, Su Jin Lee, Seong Yong Park
BACKGROUND: We evaluated glucose transporter type 1 (GLUT1) and carbonic anhydrase IX (CAIX) expression, together with volume-based(18)F-fluorodeoxyglucose positron emission tomography (FDG-PET) parameters, in non-small cell lung cancer (NSCLC) patients, and examined the prognostic significance of those parameters according to its histologic subtype. METHOD: A total of 269 patients, who underwent surgical resection for NSCLC, were reviewed retrospectively. Metabolic tumor volume (MTV) and total lesion glycolysis (TLG) values were measured by preoperative (18)F-fluorodeoxyglucose positron emission tomography computed tomography...
February 2017: Lung Cancer: Journal of the International Association for the Study of Lung Cancer
https://www.readbyqxmd.com/read/28207990/carbonic-anhydrase-and-urease-inhibitory-potential-of-various-plant-phenolics-using-in-vitro-and-in-silico-methods
#12
Abdur Rauf, Muslim Raza, Muhammad Saleem, Ufuk Ozgen, Esen Sezen Karaoglan, Gulin Renda, Erhan Palaska, Ilkay Erdogan Orhan
Plant phenolics are known to display many pharmacological activities. In the current study, eight phenolic compounds [e.g. luteolin 5-O-β-glucoside (1), methyl rosmarinate (2), apigenin (3), vicenin 2 (4), lithospermic acid (5), soyasaponin II (6), rubiadin 3-O-β-primeveroside (7), 4(3,4dihydroxybenzoyloxymethyl)phenyl-O-β-D-glucopyranoside (8)] isolated from various plant species were tested at 0.2 mM against carbonic anhydrase-II (CA-II) and urease using microtiter assays. Urease inhibition rate for compounds 1-8 ranged between 5...
February 16, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/28204987/the-effects-of-topical-carbonic-anhydrase-inhibitor-in-treatment-of-nystagmus
#13
Ebru Demet Aygit, Osman Bulut Ocak, Aslı İnal, Korhan Fazıl, Serpil Akar, Birsen Gokyigit
OBJECTIVE: To evaluate the effects of topical carbonic anhydrase inhibitor (CAI), brinzolamide (Azopt), for treatment of nystagmus patients. MATERIALS AND METHODS: Patients who used Brinzolamide for treatment of nystagmus were retrospectively analyzed. 23 patients were included in this study. The patients' diagnosis were idiopathic infantile nystagmus 18 (78.2%) and oculocutaneous albinism in 5 (21.8%). Azopt eye drop was used in both eyes, 3 times a day. Detailed eye examination in all of the patients before treatment and repeated at the end of the first week after treatment...
February 15, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28204970/a-carbonic-anhydrase-serves-as-an-important-acid-base-regulator-in-pacific-oyster-crassostrea-gigas-exposed-to-elevated-co2-implication-for-physiological-responses-of-mollusk-to-ocean-acidification
#14
Xiudan Wang, Mengqiang Wang, Zhihao Jia, Limei Qiu, Lingling Wang, Anguo Zhang, Linsheng Song
Carbonic anhydrases (CAs) have been demonstrated to play an important role in acid-base regulation in vertebrates. However, the classification and modulatory function of CAs in marine invertebrates, especially their responses to ocean acidification remain largely unknown. Here, a cytosolic α-CA (designated as CgCAII-1) was characterized from Pacific oyster Crassostrea gigas and its molecular activities against CO2 exposure were investigated. CgCAII-1 possessed a conserved CA catalytic domain, with high similarity to invertebrate cytoplasmic or mitochondrial α-CAs...
February 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28204822/melittin-inhibits-osteoclast-formation-through-the-downregulation-of-the-rankl-rank-signaling-pathway-and-the-inhibition-of-interleukin-1%C3%AE-in-murine-macrophages
#15
Jung-Yoon Choe, Seong-Kyu Kim
Melittin is a major toxic component of bee venom (Apis mellifera). It is not known whether melittin is involved in bone metabolism and osteoclastogenesis. The aim of this study was to determine the role of melittin in the regulation of osteoclastogenesis. In vitro osteoclastogenesis assays were performed using mouse RAW 264.7 cells and bone marrow-derived macrophages (BMMs) treated with receptor activator of nuclear factor-κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF). Morphologic and functional analyses for osteoclast-like multinucleated cells (MNCs) were performed by tartrate-resistant acid phosphatase (TRAP) staining, F-actin staining and pit formation methods...
March 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28204703/effect-of-dietary-supplementation-of-organic-or-inorganic-zinc-on-carbonic-anhydrase-activity-in-eggshell-formation-and-quality-of-aged-laying-hens
#16
Y N Zhang, H J Zhang, J Wang, H Y Yue, X L Qi, S G Wu, G H Qi
No abstract text is available yet for this article.
February 16, 2017: Poultry Science
https://www.readbyqxmd.com/read/28198796/carbonic-anhydrase-i-and-ii-autoantibodies-in-beh%C3%A3-et-s-disease
#17
Ahmet Mentese, Ahmet Alver, Selim Demir, Aysegul Sumer, Serap Ozer Yaman, Murat Karkucak, Arzu Aydin Capkin, Diler Us Altay, Ibrahim Turan
BACKGROUND: Behçet's disease is a vasculitis, seen more frequently around the Mediterranean and the Far East, and evinces with oral and genital ulcerations, skin lesions and uveitis. Carbonic anhydrase (CA) is a metalloenzyme which is widely distributed in the living world, and it is essential for the regulation of acid-base balance. Anti-CA antibodies have been reported in many disorders, such as systemic lupus erythematosus, Sjögren's syndrome, rheumatoid arthritis, endometriosis, idiopathic chronic pancreatitis, type 1 diabetes and Graves' disease...
December 3, 2016: Acta Reumatológica Portuguesa
https://www.readbyqxmd.com/read/28192979/structural-characterization-of-native-proteins-and-protein-complexes-by-electron-ionization-dissociation-mass-spectrometry
#18
Huilin Li, Yuewei Sheng, William McGee, Michael Cammarata, Dustin Holden, Joseph A Loo
Mass spectrometry (MS) has played an increasingly important role in the identification and structural and functional characterization of proteins. In particular, the use of tandem mass spectrometry has afforded one of the most versatile methods to acquire structural information for proteins and protein complexes. The unique nature of electron capture dissociation (ECD) for cleaving protein backbone bonds while preserving noncovalent interactions has made it especially suitable for the study of native protein structures...
February 22, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28192486/ocean-acidification-modulates-expression-of-genes-and-physiological-performance-of-a-marine-diatom
#19
Yahe Li, Shufang Zhuang, Yaping Wu, Honglin Ren, Fangyi Chen, Xin Lin, Kejian Wang, John Beardall, Kunshan Gao
Ocean Acidification (OA) is known to affect various aspects of physiological performances of diatoms, but little is known about the underlining molecular mechanisms involved. Here, we show that in the model diatom Phaeodactylum tricornutum, the expression of key genes associated with photosynthetic light harvesting as well as those encoding Rubisco, carbonic anhydrase, NADH dehydrogenase and nitrite reductase, are modulated by OA (1000 μatm, pHnbs 7.83). Growth and photosynthetic carbon fixation were enhanced by elevated CO2...
2017: PloS One
https://www.readbyqxmd.com/read/28188726/the-roles-of-v-type-h-atpase-and-na-k-atpase-in-energizing-k-and-h-transport-in-larval-drosophila-gut-epithelia
#20
Natalie M D'Silva, Andrew Donini, Michael J O'Donnell
We analyzed V-type H(+)-ATPase (VA) and Na(+)/K(+)-ATPase (NKA) along the caeca and midgut of third instar Drosophila larvae using immunohistochemistry and ATPase activity assays. Corresponding H(+) and K(+) fluxes were characterized using the Scanning Ion-Selective Electrode Technique (SIET), and the roles of transport ATPases in energizing ion transport across the larval gut were investigated by basal application of bafilomycin, a VA inhibitor, and ouabain, a NKA inhibitor. Addition of bafilomycin led to a decrease in H(+) absorption along the caeca and midgut except at the copper cells and large flat cell zone of the middle midgut...
February 7, 2017: Journal of Insect Physiology
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