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https://www.readbyqxmd.com/read/29444330/increased-species-assignment-of-filamentous-fungi-using-maldi-tof-ms-coupled-with-a-simplified-sample-processing-and-an-in-house-library
#1
M E Zvezdanova, P Escribano, A Ruiz, M C Martínez-Jiménez, T Peláez, A Collazos, J Guinea, E Bouza, B Rodríguez-Sánchez
In this study we evaluated the capacity of MALDI-TOF MS (Bruker Daltonics, Bremen, Germany) to identify clinical mould isolates. We focused on two aspects of MALDI-TOF MS identification: the sample processing and the database. Direct smearing of the sample was compared with a simplified processing consisting of mechanical lysis of the moulds followed by a protein extraction step. Both methods were applied to all isolates and the Filamentous Fungi Library 1.0 (Bruker Daltonics) was used for their identification...
February 9, 2018: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29444211/optimized-protocol-of-rbcs-lysis-for-immunophenotypic-analysis-in-the-peripheral-blood-of-tree-shrew
#2
Xintong Li, Yufen Tao, Jiansheng Liu, Wei Lu, Hongqi Liu, Jiejie Dai
No abstract text is available yet for this article.
February 12, 2018: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29442653/effect-of-titania-concentration-in-bioglass-tio%C3%A2-nanostructures-and-its-in-vitro-biological-property-assessment
#3
P Bargavi, S Chitra, D Durgalakshmi, P Rajashree, S Balakumar
Bioglass 45S5 (45% SiO2-24.5% NaO-24.5% CaO-6% P2O5) is a unique bioactive material, which is being used for bone and dental substitution. This system has been highly preferred for its osteoconductive and osteoinductive performance. Despite its attractive bioactivity, there are limitations in using this material for orthopedic and dental applications due to its poor processability and mechanical strength. To improve the load-sharing and stress distribution, TiO2 nanoparticles have been introduced into the nanoBioglass (nBG) by sol-gel method...
July 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29441828/fluoride-associated-ultrastructural-changes-and-apoptosis-in-human-renal-tubule-a-pilot-study
#4
J A Quadri, S Sarwar, A Sinha, M Kalaivani, A K Dinda, A Bagga, T S Roy, T K Das, A Shariff
The susceptibility of the kidneys to fluoride toxicity can largely be attributed to its anatomy and function. As the filtrate moves along the complex tubular structure of each nephron, it is concentrated in the proximal and distal tubules and collecting duct. It has been frequently observed that the children suffering from renal impairments also have some symptoms of dental and skeletal fluorosis. The findings suggest that fluoride somehow interferes with renal anatomy and physiology, which may lead to renal pathogenesis...
January 1, 2018: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/29440739/pyroptosis-induced-by-enterovirus-71-and-coxsackievirus-b3-infection-affects-viral-replication-and-host-response
#5
Yan Wang, Ying Qin, Tianying Wang, Yang Chen, Xiujuan Lang, Jia Zheng, Shuoyang Gao, Sijia Chen, Xiaoyan Zhong, Yusong Mu, Xiaoyu Wu, Fengming Zhang, Wenran Zhao, Zhaohua Zhong
Enterovirus 71 (EV71) is the primary causative pathogen of hand, foot, and mouth disease (HFMD), affecting children with severe neurological complications. Pyroptosis is a programmed cell death characterized by cell lysis and inflammatory response. Although proinflammatory response has been implicated to play important roles in EV71-caused diseases, the involvement of pyroptosis in the pathogenesis of EV71 is poorly defined. We show that EV71 infection induced caspase-1 activation. Responding to the activation of caspase-1, the expression and secretion of both IL-1β and IL-18 were increased in EV71-infected cells...
February 13, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29437789/targeted-bite-expression-by-an-oncolytic-vector-augments-therapeutic-efficacy-against-solid-tumors
#6
Tobias Speck, Johannes Pw Heidbuechel, Rūta Veinalde, Dirk Jaeger, Christof von Kalle, Claudia R Ball, Guy Ungerechts, Christine E Engeland
PURPOSE: Immunotherapy with bispecific T cell engagers has achieved striking success against hematological malignancies, but efficacy against solid tumors has been limited. We hypothesized that oncolytic measles viruses encoding bispecific T cell engagers (MV-BiTEs) represent a safe and effective treatment against solid tumors through local BiTE expression, direct tumor cell lysis and in situ tumor vaccination. EXPERIMENTAL DESIGN: To test this hypothesis, we generated MV-BiTEs from the Edmonston B vaccine strain to target two model antigens...
February 6, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29434608/a-case-of-type-2-hypersensitivity-to-rasburicase-diagnosed-with-a-natural-killer-cell-activation-assay
#7
Sébastien Viel, Rémi Pescarmona, Alexandre Belot, Audrey Nosbaum, Christine Lombard, Thierry Walzer, Frédéric Bérard
Drug hypersensitivity reactions can lead to different clinical pictures depending on the underlying immunological mechanism. Diagnosis tests are already available to assess the most frequent drugs hypersensitivity reactions, which are mediated by specific IgE or T cells. However, it remains challenging to diagnose type 2 hypersensitivity reactions (T2HR), which can lead to severe cytopenia and liver failure. Here, we describe a case of T2HR to rasburicase, an uricolytic agent used to prevent tumor lysis syndrome...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29434593/leishmania-donovani-inhibitor-of-serine-peptidases-2-mediated-inhibition-of-lectin-pathway-and-upregulation-of-c5ar-signaling-promote-parasite-survival-inside-host
#8
Sudha Verma, Abhishek Mandal, Md Yousuf Ansari, Ajay Kumar, Kumar Abhishek, Ayan Kumar Ghosh, Ashish Kumar, Vinod Kumar, Sushmita Das, Pradeep Das
Leishmania donovani, the causative agent of Indian visceral leishmaniasis has to face several barriers of the immune system inside the mammalian host for its survival. The complement system is one of the first barriers and consists of a well-balanced network of proteases including S1A family serine proteases (SPs). Inhibitor of serine peptidases (ISPs) is considered as inhibitor of S1A family serine peptidases and is reported to be present in trypanosomes, including Leishmania. In our previous study, we have deciphered the role of ISPs [LdISP1 and L...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29434479/osteoarthritis-and-the-complement-cascade
#9
REVIEW
Sandeep Silawal, Jakob Triebel, Thomas Bertsch, Gundula Schulze-Tanzil
Accumulating evidence demonstrates that complement activation is involved in the pathogenesis of osteoarthritis (OA). However, the intimate complement regulation and cross talk with other signaling pathways in joint-associated tissues remain incompletely understood. Recent insights are summarized and discussed here, to put together a more comprehensive picture of complement involvement in OA pathogenesis. Complement is regulated by several catabolic and inflammatory mediators playing a key role in OA. It seems to be involved in many processes observed during OA development and progression, such as extracellular cartilage matrix (ECM) degradation, chondrocyte and synoviocyte inflammatory responses, cell lysis, synovitis, disbalanced bone remodeling, osteophyte formation, and stem cell recruitment, as well as cartilage angiogenesis...
2018: Clinical Medicine Insights. Arthritis and Musculoskeletal Disorders
https://www.readbyqxmd.com/read/29434405/inclusion-of-2-mercaptoethanol-in-lysis-buffer-could-interfere-with-isolation-of-high-molecular-weight-dna-from-freshwater-microalgae
#10
Willy Yee, Janushree Nanda Kumar, Priya Devi Muthusamy
2-mercaptoethanol (2-ME), alongside polyvinylpyrrolidone is commonly used in plant DNA extractions to deal with polyphenols, which could interfere with extraction and downstream applications. 2-ME is also commonly used to denature proteins and nucleases, especially RNAses. On the contrary, we found that the presence of 2-ME in lysis buffer interfered with DNA extraction from 12 strains of freshwater microalgae, resulting in DNA with poor integrity. We also found that the TNES-urea buffer, commonly used for preservation and DNA extraction from fish, appears as effective as the SDS and CTAB buffer for some microalgae strains...
March 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29430990/the-metalloprotease-adam10-a-disintegrin-and-metalloprotease-10-undergoes-rapid-postlysis-autocatalytic-degradation
#11
Tobias Brummer, Martina Pigoni, Armando Rossello, Huanhuan Wang, Peter J Noy, Michael G Tomlinson, Carl P Blobel, Stefan F Lichtenthaler
The transmembrane protein, ADAM10 (a disintegrin and metalloprotease 10), has key physiologic functions-for example, during embryonic development and in the brain. During transit through the secretory pathway, immature ADAM10 (proADAM10) is converted into its proteolytically active, mature form (mADAM10). Increasing or decreasing the abundance and/or activity of mADAM10 is considered to be a therapeutic approach for the treatment of such diseases as Alzheimer's disease and cancer. Yet biochemical detection and characterization of mADAM10 has been difficult...
February 7, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29427947/analysis-of-ribosomal-rna-stability-in-dead-cells-of-wine-yeast-by-quantitative-pcr
#12
Merce Sunyer-Figueres, Chunxiao Wang, Albert Mas
During wine production, some yeasts enter a Viable But Not Culturable (VBNC) state, which may influence the quality and stability of the final wine through remnant metabolic activity or by resuscitation. Culture-independent techniques are used for obtaining an accurate estimation of the number of live cells, and quantitative PCR could be the most accurate technique. As a marker of cell viability, rRNA was evaluated by analyzing its stability in dead cells. The species-specific stability of rRNA was tested in Saccharomyces cerevisiae, as well as in three species of non-Saccharomyces yeast (Hanseniaspora uvarum, Torulaspora delbrueckii and Starmerella bacillaris)...
January 31, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29427353/rapid-reduction-of-extremely-high-kappa-free-light-chains-in-a-patient-with-myeloma-cast-nephropathy
#13
Christine L H Snozek, Theresa N Kinard, Jill Adamski
This report describes a patient with light chain myeloma and acute renal injury. Serum kappa free light chain (FLC) was extremely elevated, >33,000 mg/dL. Treatment with therapeutic plasma exchange (TPE) started day 2 for biopsy-confirmed cast nephropathy. Bortezomib-containing chemotherapy was initiated on day 5, and hemodialysis for tumor lysis syndrome on day 7. TPE alone decreased kappa FLC >70% by day 5, indicating direct FLC removal was successful in this patient. A total of 25 TPE procedures were performed in a 31-day hospitalization...
February 10, 2018: Journal of Clinical Apheresis
https://www.readbyqxmd.com/read/29426480/development-of-a-direct-pcr-assay-to-detect-taenia-multiceps-eggs-isolated-from-dog-feces
#14
Ning Wang, Yu Wang, Qinghua Ye, Yingdong Yang, Jie Wan, Cheng Guo, Jiafei Zhan, Xiaobin Gu, Weimin Lai, Yue Xie, Xuerong Peng, Guangyou Yang
Taenia multiceps is a tapeworm that leads to the death of livestock, resulting in major economic losses worldwide. The adult stage of this parasite invades the small intestine of dogs and other canids. In the present study, we developed a direct PCR assay to detect T. multiceps eggs isolated from dog feces to help curb further outbreaks. The genomic DNA was rapidly released using a lysis buffer and the PCR reaction was developed to amplify a 433-bp fragment of the T. multiceps mitochondrial gene encoding NADH dehydrogenase subunit 5 (nad5) from eggs isolated from dog feces...
February 15, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29425691/the-intracellular-region-of-silkworm-cadherin-like-protein-is-not-necessary-to-mediate-the-toxicity-of-bacillus-thuringiensis-cry1aa-and-cry1ab-toxins
#15
Haruka Endo, Satomi Adegawa, Shingo Kikuta, Ryoichi Sato
The cadherin-like protein in lepidopteran insects, known as a receptor for Bacillus thuringiensis Cry1A toxins, is a single-pass membrane protein that can be divided into extracellular and intracellular regions. The extracellular region is important for toxin binding and oligomerization, whereas the role of the intracellular region during Cry1A intoxication is unclear. In the present study, we generated a deletion mutant of Bombyx mori cadherin-like protein (BtR175) that lacked the intracellular region to investigate its role in mediating Cry1A toxicity...
February 6, 2018: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29425312/risk-factors-for-early-intraocular-pressure-elevation-after-cataract-surgery-in-a-cohort-of-united-states-veterans
#16
Kaushik Annam, Allison J Chen, Irene M Lee, Alfred A Paul, Jorge J Rivera, Paul B Greenberg
Introduction: Cataract surgery is the most frequently performed surgery in the Veterans Health Administration (VHA). A well-known complication is a transient but potentially harmful elevation in intraocular pressure (IOP) within the 24-h postoperative period. The purpose of this study is to investigate the risk factors for IOP elevation 1 d after cataract surgery in a cohort of United States (US) veterans. Materials and Methods: The study included 784 patients who underwent cataract surgery between April 2013 and April 2016 at a single Veterans Affairs medical center in Providence, RI...
February 7, 2018: Military Medicine
https://www.readbyqxmd.com/read/29423464/inertial-ordering-assisted-droplet-microfluidics-for-high-throughput-single-cell-rna-sequencing
#17
Hui-Sung Moon, Kwanghwi Je, Jae-Woong Min, Donghyun Park, Kyung-Yeon Han, Seung-Ho Shin, Woong-Yang Park, Chang Eun Yoo, Shin-Hyun Kim
Single-cell RNA-seq reveals the cellular heterogeneity inherent in the population of cells, which is very important in many clinical and research applications. Recent advances in droplet microfluidics have achieved the automatic isolation, lysis, and labeling of single cells in droplet compartments without complex instrumentation. However, barcoding errors occurring in the cell encapsulation process because of the multiple-beads-in-droplet and insufficient throughput because of the low concentration of beads for avoiding multiple-beads-in-a-droplet remain important challenges for precise and efficient expression profiling of single cells...
February 9, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29421063/targeted-delivery-of-snx-2112-by-polysaccharide-modified-graphene-oxide-nanocomposites-for-treatment-of-lung-cancer
#18
Xuan Liu, Xiaozhen Cheng, Fengzhe Wang, Longbao Feng, Yu Wang, Yanfang Zheng, Rui Guo
Graphene oxide (GO) is a promising material for biomedical applications, particularly in drug delivery, due to its exceptional chemical and physical properties. In this work, an innovative GO-based carrier was developed by modifying GO with chitosan (CHI) to improve the biocompatibility, and followed by the conjugation of hyaluronic acid (HA), the target ligand for CD44, to realize the specific recognition of tumor cells and improve the efficiency of anti-tumor drug delivery. The resulting product GO-CHI-HA was loaded with an anti-cancer drug SNX-2112, which is the Hsp90 inhibitor...
April 1, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29420123/talimogene-laherparepvec-first-in-class-oncolytic-virotherapy
#19
Robert M Conry, Brian Westbrook, Svetlana McKee, Timothy Graham Norwood
Oncolytic viruses represent a novel drug class in which native or modified viruses mediate tumor regression through selective replication within and lysis of tumor cells as well as induction of systemic antitumor immunity capable of eradicating tumor at distant, uninjected sites. Talimogene laherparepvec (TVEC) is a type I herpes simplex virus genetically modified to preferentially replicate in tumor cells, enhance antigen loading of MHC class I molecules and express granulocyte-macrophage colony-stimulating factor to increase tumor-antigen presentation by dendritic cells...
February 8, 2018: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/29417407/a-systematic-review-of-ultrasound-accelerated-catheter-directed-thrombolysis-in-the-treatment-of-deep-vein-thrombosis
#20
REVIEW
Yadong Shi, Wanyin Shi, Liang Chen, Jianping Gu
To review the clinical evidence for ultrasound-accelerated catheter-directed thrombolysis (UACDT) using the EKOS system in the treatment of deep vein thrombosis (DVT) in terms of case selection, procedural outcomes, clinical outcomes and safety outcomes. A systematic literature search strategy was used to identify the use of the EKOS system in the treatment of DVT using the following electronic databases: MEDLINE, EMBASE, the Cochrane databases and the Web of Science. The references in the relevant literature were also screened...
February 7, 2018: Journal of Thrombosis and Thrombolysis
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