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https://www.readbyqxmd.com/read/27921389/superiority-of-sds-lysis-over-saponin-lysis-for-direct-bacterial-identification-from-positive-blood-culture-bottle-by-maldi-tof-ms
#1
Yvan Caspar, Cécile Garnaud, Mariya Raykova, Sébastien Bailly, Marie Bidart, Danièle Maubon
PURPOSE: Fast species diagnosis has an important health care impact, as rapid and specific antibacterial therapy is of clear benefit for patient's outcome. Here we propose a new protocol for species identification directly from positive blood cultures. EXPERIMENTAL DESIGN: We compared four in-house protocols for bacterial identification by mass spectrometry directly from clinical positive blood cultures evaluating 2 lytic agents, SDS and saponin, and 2 protein extraction schemes, fast (FP) and long (LP)...
December 6, 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/27920762/artificial-intelligence-vs-statistical-modeling-and-optimization-of-continuous-bead-milling-process-for-bacterial-cell-lysis
#2
Shafiul Haque, Saif Khan, Mohd Wahid, Sajad A Dar, Nipunjot Soni, Raju K Mandal, Vineeta Singh, Dileep Tiwari, Mohtashim Lohani, Mohammed Y Areeshi, Thavendran Govender, Hendrik G Kruger, Arshad Jawed
For a commercially viable recombinant intracellular protein production process, efficient cell lysis and protein release is a major bottleneck. The recovery of recombinant protein, cholesterol oxidase (COD) was studied in a continuous bead milling process. A full factorial response surface methodology (RSM) design was employed and compared to artificial neural networks coupled with genetic algorithm (ANN-GA). Significant process variables, cell slurry feed rate (A), bead load (B), cell load (C), and run time (D), were investigated and optimized for maximizing COD recovery...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27920758/effectiveness-of-f18-fimbrial-antigens-released-by-a-novel-autolyzed-salmonella-expression-system-as-a-vaccine-candidate-against-lethal-f18-stec-infection
#3
Gayeon Won, John H Lee
Porcine edema disease (ED) caused by Shiga toxin 2e producing Escherichia coli expressing F18ab(+) fimbriae (F18ab(+)STEC) frequently occurs in post-weaned piglets, resulting in a significant economic loss in swine industries worldwide. In the present study, we proposed an efficient prevention scheme against ED in which the attenuated Salmonella Typhimurium inactivated by the E-mediated cell lysis to deliver target antigens, FedF and FedA, which function in fimbrial-mediated adhesion and as a major subunit of F18ab+fimbriae, respectively...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27920582/surgeons-early-experience-with-the-acessa%C3%A2-procedure-gaining-proficiency-with-new-technology
#4
Kelli M Braun, Mark Sheridan, Erin Z Latif, Lexy Regush, Anet Maksymowicz, Laura Weins, Mohamed A Bedaiwy, Nerissa Tyson, Marilyn J Davidson, Barry H Sanders
PURPOSE: Successful adoption of a new surgical procedure varies among practicing surgeons, and skill acquisition depends on the surgeon's innate ability, the complexity of the technique, and training. We report intraoperative and near-term postoperative outcomes from the Acessa procedure conducted by minimally invasive gynecologic surgeons new to Acessa, and report the surgeons' experiences during the training period. PATIENTS AND METHODS: The study was designed as a postmarket, prospective, single-arm, multicenter analysis of operative and early postoperative outcomes after proctored surgical training with the Acessa device and procedure (laparoscopic ultrasound-guided radiofrequency volumetric thermal ablation of symptomatic fibroids) in premenopausal, menstruating women as conducted in community and university hospitals in the USA and Canada...
2016: International Journal of Women's Health
https://www.readbyqxmd.com/read/27919998/high-risk-of-tumor-lysis-syndrome-in-symptomatic-patients-with-multiple-myeloma-with-renal-dysfunction-treated-with-bortezomib
#5
Kana Oiwa, Mihoko Morita, Shinji Kishi, Miyuki Okura, Toshiki Tasaki, Yasufumi Matsuda, Katsunori Tai, Naoko Hosono, Takanori Ueda, Takahiro Yamauchi
BACKGROUND/AIM: Tumor lysis syndrome (TLS) is a life-threatening complication associated with cancer chemotherapy. We retrospectively evaluated the risk of developing TLS in patients with symptomatic multiple myeloma undergoing chemotherapy. PATIENTS AND METHODS: Sixty-four patients (median age=71 years, range=48-87 years, 35 males/29 females) who were treated at our Institution between April 2006 and December 2015 were evaluated. RESULTS: A total of 124 chemotherapy courses were administered, of which 63 courses were bortezomib-based regimens and 34 courses were immunomodulatory drug (IMiD)-based regimens...
December 2016: Anticancer Research
https://www.readbyqxmd.com/read/27919263/derivation-of-chicken-induced-pluripotent-stem-cells-tolerant-to-newcastle-disease-virus-induced-lysis-through-multiple-rounds-of-infection
#6
Leonardo Susta, Ying He, Jessica M Hutcheson, Yangqing Lu, Franklin D West, Steven L Stice, Ping Yu, Zaid Abdo, Claudio L Afonso
BACKGROUND: Newcastle disease (ND), caused by Newcastle disease virus (NDV), is a devastating disease of poultry and wild birds. ND is prevented by rigorous biocontainment and vaccination. One potential approach to prevent spread of the virus is production of birds that show innate resistance to NDV-caused disease. Induced pluripotent stem cell (iPSC) technology allows adult cells to be reprogrammed into an embryonic stem cell-like state capable of contributing to live offspring and passing on unique traits in a number of species...
December 5, 2016: Virology Journal
https://www.readbyqxmd.com/read/27917156/inhibitory-effects-of-macrotetrolides-from-streptomyces-spp-on-zoosporogenesis-and-motility-of-peronosporomycete-zoospores-are-likely-linked-with-enhanced-atpase-activity-in-mitochondria
#7
Md Tofazzal Islam, Hartmut Laatsch, Andreas von Tiedemann
The release of zoospores from sporangia and motility of the released zoospores are critical in the disease cycle of the Peronosporomycetes that cause devastating diseases in plants, fishes, animals and humans. Disruption of any of these asexual life stages eliminates the possibility of pathogenesis. In the course of screening novel bioactive secondary metabolites, we found that extracts of some strains of marine Streptomyces spp. rapidly impaired motility and caused subsequent lysis of zoospores of the grapevine downy mildew pathogen Plasmopara viticola at 10 μg/ml...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27914209/methodological-flaws-introduce-strong-bias-into-molecular-analysis-of-microbial-populations
#8
Niclas Krakat, Reshma Anjum, Burak Demirel, Patrick Schröder
AIMS: Here we report how different cell disruption methods, PCR primers and in-silico analyses can seriously bias results from microbial population studies, with consequences for the credibility and reproducibility of the findings. Our results emphasize the pitfalls of commonly used experimental methods that can seriously weaken the interpretation of results. METHODS AND RESULTS: Four different cell lysis methods, three commonly used primer pairs and various computer-based analyses were applied to investigate the microbial diversity of a fermentation sample composed of chicken dung...
December 3, 2016: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/27911388/high-throughput-real-time-dual-readout-testing-of-intracellular-antimicrobial-activity-and-eukaryotic-cell-cytotoxicity
#9
Lucius Chiaraviglio, Yoon-Suk Kang, James E Kirby
Traditional measures of intracellular antimicrobial activity and eukaryotic cell cytotoxicity rely on endpoint assays. Such endpoint assays require several additional experimental steps prior to readout, such as cell lysis, colony forming unit determination, or reagent addition. When performing thousands of assays, for example, during high-throughput screening, the downstream effort required for these types of assays is considerable. Therefore, to facilitate high-throughput antimicrobial discovery, we developed a real-time assay to simultaneously identify inhibitors of intracellular bacterial growth and assess eukaryotic cell cytotoxicity...
November 16, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27909706/out-of-plane-integration-of-a-multimode-optical-fiber-for-single-particle-cell-detection-at-multiple-points-on-a-microfluidic-device-with-applications-to-particle-cell-counting-velocimetry-size-discrimination-and-the-analysis-of-single-cell-lysate-injections
#10
Jalal Sadeghi, Damith E W Patabadige, Anne H Culbertson, Hamid Latifi, Christopher T Culbertson
In this paper a single particle/cell-tracking microfluidic device that integrates an out-of-plane multimode optical fiber (OP-MMF) is reported. This OP-MMF is used to generate three excitation light-lines and three detection spots using only one excitation source and one detector. It takes advantage of an optical tunneling mode to create two excitation lines in a microfluidic channel emanating from a single fiber end. This method was used to accurately count particles/cells and perform velocity measurements and size discrimination...
December 2, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27908758/thermally-triggered-release-of-the-bacteriophage-endolysin-chapk-and-the-bacteriocin-lysostaphin-for-the-control-of-methicillin-resistant-staphylococcus-aureus-mrsa
#11
Hollie Hathaway, Jude Ajuebor, Liam Stephens, Aidan Coffey, Ursula Potter, J Mark Sutton, A Toby A Jenkins
Staphylococcus aureus infections of the skin and soft tissue pose a major concern to public health, largely owing to the steadily increasing prevalence of drug resistant isolates. As an alternative mode of treatment both bacteriophage endolysins and bacteriocins have been shown to possess antimicrobial efficacy against multiple species of bacteria including otherwise drug resistant strains. Despite this, the administration and exposure of such antimicrobials should be restricted until required in order to discourage the continued evolution of bacterial resistance, whilst maintaining the activity and stability of such proteinaceous structures...
November 28, 2016: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/27904893/assay-for-listeria-monocytogenes-cells-in-whole-blood-using-isotachophoresis-and-recombinase-polymerase-amplification
#12
Charbel Eid, Juan G Santiago
We present a new approach which enables lysis, extraction, and detection of inactivated Listeria monocytogenes cells from blood using isotachophoresis (ITP) and recombinase polymerase amplification (RPA). We use an ITP-compatible alkaline and proteinase K approach for rapid and effective lysis. We then perform ITP purification to separate bacterial DNA from whole blood contaminants using a microfluidic device that processes 25 μL sample volume. Lysis, mixing, dispensing, and on-chip ITP purification are completed in a total of less than 50 min...
December 1, 2016: Analyst
https://www.readbyqxmd.com/read/27903527/standardized-flow-cytometry-for-highly-sensitive-mrd-measurements-in-b-cell-acute-lymphoblastic-leukemia
#13
Prisca Theunissen, Ester Mejstrikova, Lukasz Sedek, Alita J van der Sluijs-Gelling, Giuseppe Gaipa, Marius Bartels, Elaine Sobral da Costa, Michaela Kotrova, Michaela Novakova, Edwin Sonneveld, Chiara Buracchi, Paola Bonaccorso, Elen Oliviera, Jeroen G Te Marvelde, Tomasz Szczepanski, Ludovic Lhermitte, Ondrej Hrusak, Quentin Lecrevisse, Georgiana Emilia Grigore, Eva Fronkova, Jan Trka, Monika Brüggemann, Alberto Orfao, Jacques J M van Dongen, Vincent H J van der Velden
A fully-standardized EuroFlow 8-color antibody panel and laboratory procedure was stepwise designed to measure minimal residual disease (MRD) in B-cell precursor (BCP) acute lymphoblastic leukemia (ALL) patients with a sensitivity of ≤10(-5), comparable to real-time quantitative (RQ)-PCR-based MRD detection via antigen-receptor rearrangements. Leukocyte markers and the corresponding antibodies and fluorochromes were selected based on their contribution in separating BCP-ALL cells from normal/regenerating BCP cells in multidimensional principal component analyses...
November 30, 2016: Blood
https://www.readbyqxmd.com/read/27903454/effects-of-frequently-used-pharmaceutical-excipients-on-the-organic-cation-transporters-1-3-and-peptide-transporters-1-2-stably-expressed-in-mdckii-cells
#14
Marcus Otter, Stefan Oswald, Werner Siegmund, Markus Keiser
There is ample evidence that pharmaceutical excipients, which are supposed to be pharmacologically inactive, have an impact on drug metabolism and efflux transport. So far, little is known whether they also modulate uptake transporter proteins. We have recently shown that commonly used solubilizing agents exert significant effects on the function of organic anion uptake transporting polypeptides. Therefore, we investigated in this study the influence of frequently used pharmaceutical excipients on the transport activity of organic cation transporters OCT1, OCT2 and OCT3 and the peptide transporters PEPT1 and PEPT2...
November 26, 2016: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/27902355/the-use-of-human-umbilical-cord-blood-serum-is-beneficial-for-the-continuous-production-of-hepatitis-c-virus
#15
Xiao-Yang Jiao, Rineke H G Steenbergen, D Lorne Tyrrell
In this study we investigated if human umbilical cord blood serum (CBS) is a suitable replacement for fetal bovine serum (FBS) in cultures of human hepatoma cell line Huh7.5, particularly regarding its capacity to maintain high growth rates, differentiation status and its ability to support robust HCV infection. Generally, CBS cultured Huh7.5 cells remained comparable to FBS cultured cells, and proliferated equally well. Albumin secretion, a hepatocyte differentiation marker, had increased 8x in CBS, however, most other hepatocyte markers we tested had not changed...
October 24, 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27902200/haloprofundus-marisrubri-gen-nov-sp-nov-an-extremely-halophilic-archaeon-isolated-from-the-discovery-deep-brine-seawater-interface-in-the-red-sea
#16
Guishan Zhang, Jingang Gu, Ruifu Zhang, Mamoon Rashid, Mohamed Fauzi Haroon, Weibing Xun, Zhiyong Ruan, Xiuzhu Dong, Ulrich Stingl
We isolated a Gram-staining-negative, pink-pigmented, motile, pleomorphic, extremely halophilic archaeon from the brine-seawater interface of Discovery Deep in the Saudi Arabian Red Sea. This strain, designated SB9T, is capable of growth within a wide range of temperatures and salinity concentrations, but requires MgCl2. Cells lyse in distilled water, but at 7.0% NaCl (w/v) cell lysis is prevented. The major polar lipids from strain SB9T are phosphatidylglycerol (PG), phosphatidylglycerorophosphate-methylester (PGP-Me), sulfated mannosyl glucosyl diether (S-DGD-1), mannosyl glucosyl diether (DGD-1), unidentified glycolipid (GL1) and unidentified phospholipids (UPL1 and UPL2)...
October 9, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27900590/assessment-of-oxidative-nitrative-modifications-of-plasma-proteins-selected-rotem-parameters-and-kinetics-of-fibrinogen-polymerization-in-patients-with-multiple-myeloma-at-diagnosis
#17
W Nowak, J Treliński, K Chojnowski, J Matczak, M Robak, M Misiewicz, P Nowak
Patients with multiple myeloma (MM) are at increased risk of thrombosis. Growing evidence indicates that oxidative and nitrative modifications of proteins, including fibrinogen, may lead to changes in hemostasis. The study compares samples from patients with MM at diagnosis and healthy volunteers with regard to the oxidative/nitrative modifications of proteins, ROTEM and thrombin-catalyzed fibrin polymerization. The content of carbonyl groups in plasma proteins of patients with MM was significantly higher than in controls (2...
January 2017: Medical Oncology
https://www.readbyqxmd.com/read/27900227/urachal-cyst-causing-small-bowel-obstruction-in-an-adult-with-a-virgin-abdomen
#18
Michael P O'Leary, Zane W Ashman, David S Plurad, Dennis Y Kim
Introduction. A patent urachus is a rare congenital or acquired pathology, which can lead to complications later in life. We describe a case of urachal cystitis as the etiology of small bowel obstruction in an adult without prior intra-abdominal surgery. Case Report. A 64-year-old male presented to the acute care surgery team with a 5-day history of right lower quadrant abdominal pain, distention, nausea, and vomiting. He had a two-month history of urinary retention and his past medical history was significant for benign prostate hyperplasia...
2016: Case Reports in Surgery
https://www.readbyqxmd.com/read/27900101/neoplastic-fever-in-patients-with-bone-and-soft-tissue-sarcoma
#19
Tomoki Nakamura, Akihiko Matsumine, Takao Matsubara, Kunihiro Asanuma, Akihiro Sudo
The development of fever is a common complication in the clinical course of cancer. If all other potential causes of fever are excluded, the possibility of neoplastic fever should be considered. The aim of the present study was to determine the incidence of neoplastic fever in patients with bone and soft tissue sarcomas. Between January 2009 and December 2014, 195 patients with bone and soft tissue sarcoma (111 men and 84 women; mean age, 55 years) were admitted to the Department of Orthopaedic Surgery of Mie University Graduate School of Medicine (Tsu, Japan)...
November 2016: Molecular and Clinical Oncology
https://www.readbyqxmd.com/read/27899238/goat-umbilical-cord-cells-are-permissive-to-small-ruminant-lentivirus-infection-in-vitro
#20
Gabrielle R Martins, Rebeca C Marinho, Rosivaldo Q Bezerra Junior, Antoniel de O Alves, Lilia M C Câmara, Luiz C Albuquerque-Pinto, Maria F da S Teixeira
Small ruminant lentiviruses isolated from peripheral blood leukocytes and target organs can be propagated in vitro in fibroblasts derived from goat synovial membrane cells. These cells are obtained from tissues collected from embryos or fetuses and are necessary for the establishment of the fibroblast primary culture. A new alternative type of host cells, derived from goat umbilical cord, was isolated and characterized phenotypically with its main purpose being to obtain cell monolayers that could be used for the diagnosis and isolation of small ruminant lentiviruses in cell culture...
November 19, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
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