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https://www.readbyqxmd.com/read/29228300/dna-synthesis-by-fragment-assembly-using-extra-cellular-dna-delivered-by-artificial-controlled-horizontal-transfer
#1
Shinya Kaneko, Hiromi Fukushima, Misako Nakahama, Satomi Asano, Yasumasa Miyazaki, Yasunori Aizawa, Mitsuhiro Itaya
DNA synthesis in the Bacillus subtilis cells has become possible using extra-cellular DNA. Generally, purified DNAs in a test tube have been required to introduce into the host cells for molecular cloning technology in the laboratory. We have developed a cell lysis technique for natural transformation using stable extra-cellular plasmid DNAs, in which the extra-cellular plasmid DNAs are released from lysed Escherichia coli cells. DNA synthesis then proceeds by fragment assembly using the stable extracellular DNAs, without biochemical purification...
December 7, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/29227905/antimicrobial-activity-of-gallic-acid-against-food-related-pseudomonas-strains-and-its-use-as-biocontrol-tool-to-improve-the-shelf-life-of-fresh-black-truffles
#2
Elena Sorrentino, Mariantonietta Succi, Luca Tipaldi, Gianfranco Pannella, Lucia Maiuro, Marina Sturchio, Raffaele Coppola, Patrizio Tremonte
Refrigeration alone or in combination with other technologies represents the main tool used in the last decades to preserve the freshness of black truffles. This is principally due to the delicateness and vulnerability of this edible hypogeous fungus, so that other invasive preservation practices cannot be adopted. However, the proliferation of some microbial species during the cold storage still represents an unsolved problem. Pseudomonads are among the main spoiler bacteria responsible for the deterioration of refrigerated black truffles...
December 2, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29226714/-analysis-of-d-dimer-laboratory-findings-and-clinical-evaluation
#3
János Jákó
D-dimer is a product of the enzymatic degradation of the fibrinogen-fibrin molecule, and its existence is demonstrable in circulation. The test based limits may be considered as normal values. It was first thought to be a product of coagulation, then a product of lysis. High-concentration D-dimer in blood detected in thromboembolic diseases is considered to be of diagnostic value. In cases where thromboembolism was ruled out despite elevated titres but heparin (LMWH) or CLOPIDOGREL was given as a cautionary measure, we found that D-dimer values remained elevated...
December 2017: Orvosi Hetilap
https://www.readbyqxmd.com/read/29225880/tumor-lysis-syndrome-with-massive-hyperphosphatemia-and-hyperuricemia
#4
Abhilash Koratala
Tumor lysis syndrome (TLS) occurs when tumor cells release their contents into the bloodstream, typically in response to chemotherapy, leading to the characteristic findings of hyperuricemia, hyperkalemia, hyperphosphatemia, hypocalcemia, and acute kidney injury. Twinkle artifacts on renal ultrasound may indicate calcium phosphate deposits in such patients.
December 2017: Clinical Case Reports
https://www.readbyqxmd.com/read/29225869/spontaneous-tumor-lysis-syndrome-in-colon-cancer-a-case-report-and-literature-review
#5
David Sommerhalder, Amol M Takalkar, Rodney Shackelford, Prakash Peddi
Although tumor lysis syndrome is well described, it is rarely seen or suspected in solid malignancies. Early recognition of this entity is paramount in reducing morbidity and mortality. Treating physicians should be aware of this possibility in solid tumor patients with either bulky disease or extensive liver involvement.
December 2017: Clinical Case Reports
https://www.readbyqxmd.com/read/29225327/plasma-fibrin-clot-structure-and-thromboembolism-clinical-implications
#6
Michał Ząbczyk, Anetta Undas
Fibrin formed as a result of fibrinogen polymerization is a main protein component of a clot in a test tube and intravascular thrombi in vivo. Fibrin clot structure characterized by fibers diameter and pore size differs between healthy subjects and those with thromboembolic diseases, in part due to the quality and quantity of fibrinogen and the magnitude of thrombin generation. A key measure of plasma clot structure is its permeability, reflected by the Darcy constant, Ks. Reduced Ks is a typical feature of the prothrombotic fibrin clot phenotype, which is associated with faster formation of denser fibrin mesh, relatively resistant to lysis...
December 11, 2017: Polish Archives of Internal Medicine
https://www.readbyqxmd.com/read/29224166/dna-methylation-analysis-from-blood-spots-increasing-yield-and-quality-for-genome-wide-and-locus-specific-methylation-analysis
#7
Akram Ghantous, Hector Hernandez-Vargas, Zdenko Herceg
Blood represents an easily accessible human tissue for numerous research and clinical applications, including surrogate roles for biomarkers of other tissues. Dried blood spots (DBS) are space- and cost-efficient storage forms of blood while stably retaining many of its chemical constituents. Consequently, neonatal DBS are routinely collected in many countries, and their biobanks represent gold mines for research. However, the utility of DBS is restricted by the limited amount and quality of extractable biomolecules (including DNA), especially for genome-wide profiling...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224071/sequencing-of-genomes-from-environmental-single-cells
#8
Robert M Bowers, Janey Lee, Tanja Woyke
Sequencing of single bacterial and archaeal cells is an important methodology that provides access to the genetic makeup of uncultivated microorganisms. We here describe the high-throughput fluorescence-activated cell sorting-based isolation of single cells from the environment, their lysis and strand displacement-mediated whole genome amplification. We further outline 16S rRNA gene sequence-based screening of single-cell amplification products, their preparation for Illumina sequencing libraries, and finally propose computational methods for read and contig level quality control of the resulting sequence data...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29223452/antimicrobial-activity-of-bulbothrix-setschwanensis-zahlbr-hale-lichen-by-cell-wall-disruption-of-staphylococcus-aureus-and-cryptococcus-neoformans
#9
Indresh K Maurya, Samer Singh, Rupinder Tewari, Manish Tripathi, Shashi Upadhyay, Yogesh Joshi
In the present study, antimicrobial activity of a common Himalayan lichen viz. Bulbothrix setschwanensis (Zahlbr.) Hale extract in three common solvents (acetone, chloroform and methanol) was evaluated against six bacterial and seven fungal clinical strains. The acetone extract showed promising antimicrobial activity against S. aureus (1.56 mg/mL) and C. neoformans (6.25 mg/mL). Further, GC-MS analysis revealed 2,3-bis(2-methylpentanoyloxy)propyl 2-methylpentanoate and Ethyl 2-[(2R,3R,4aR,8aS)-3-hydroxy-2,3,4,4a,6,7,8,8a-octahydropyrano [3,2-b]pyran-2-yl]acetate as the predominant compounds...
December 6, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29222263/crisis-management-in-the-treatment-of-childhood-acute-lymphoblastic-leukemia-putting-right-what-can-go-wrong-emergency-complications-of-disease-and-treatment
#10
REVIEW
Rachael Hough, Ajay Vora
The improvement in overall survival in children with acute lymphoblastic leukemia (ALL) over the last 5 decades has been considerable, with around 90% now surviving long term. The risk of relapse has been reduced to such an extent that the risk of treatment-related mortality is now approaching that of mortality caused by relapse. Toxicities may also lead to the suboptimal delivery of chemotherapy (treatment delays, dose reductions, dose omissions), potentially increasing relapse risk, and short- and long-term morbidity, adding to the "burden of therapy" in an increasing number of survivors...
December 8, 2017: Hematology—the Education Program of the American Society of Hematology
https://www.readbyqxmd.com/read/29220385/secreted-dual-reporter-assay-with-gaussia-luciferase-and-the-red-fluorescent-protein-mcherry
#11
Diana Wider, Didier Picard
The availability of a wide range of reporter proteins, which can easily be quantitated, has had a major impact on many fields of biomedical research. In some experiments with tissue culture cells, it is necessary to control for differences in transfection efficiency and in other expression parameters. This requirement has been very conveniently met with the popular dual luciferase assay. Its disadvantages are the requirement for cell lysis, the inability to analyze the same cells repeatedly, and the cost, at least in its most commonly used commercial format...
2017: PloS One
https://www.readbyqxmd.com/read/29218495/systemic-epstein-barr-virus-positive-t-cell-lymphoproliferative-disorders-of-childhood-with-fulminant-leukocytosis-and-tumor-lysis-a-case-report-with-autopsy-findings
#12
Sachie Wada, Takayuki Suzuki, Koichi Kitazume, Akira Fujita, Seiichiro Shimizu
Systemic Epstein-Barr virus (EBV)-positive T-cell lymphoproliferative disorders (T-LPD) of childhood is an extremely rare disease characterized by an aggressive clinical course and very poor prognosis. We report an adolescent male with systemic EBV-positive T-LPD of childhood after primary EBV infection, resulting in a fatal clinical course within 9 days, along with autopsy findings. A 19-year-old male without an immunocompromised status presented with an acute onset of high fever, and was hospitalized for persistent fever, vomiting and diarrhea on the 5th day from onset...
December 7, 2017: International Journal of Hematology
https://www.readbyqxmd.com/read/29217384/biocompatible-sulfenamide-and-sulfonamide-derivatives-of-metformin-can-exert-beneficial-effects-on-plasma-haemostasis
#13
Magdalena Markowicz-Piasecka, Kristiina M Huttunen, Elżbieta Mikiciuk-Olasik, Joanna Sikora
As the pharmacokinetic properties of metformin are unfavourable, several analogues and prodrugs have been synthesised to improve its bioavailability. The aim of this study was to assess the plasma stability of sulfenamide and sulfonamide derivatives of metformin and establish their effects on plasma haemostasis and integrity of red blood cells (RBCs). The overall haemostasis potential was evaluated spectrophotometrically by clot formation and lysis test (CL-test). PT (Prothrombin Time) and APTT (Activated Partial Tromboplastin Time) were used to evaluate the effects if the compounds on the extrinsic and intrinsic coagulation pathway...
December 4, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29215524/urinary-tract-injury-in-gynecologic-laparoscopy-for-benign-indication-a-systematic-review
#14
Jacqueline M K Wong, Pietro Bortoletto, Jocelyn Tolentino, Michael J Jung, Magdy P Milad
OBJECTIVE: To perform a comprehensive literature review of the incidence, location, etiology, timing, management, and long-term sequelae of urinary tract injury in gynecologic laparoscopy for benign indication. DATA SOURCES: A systematic review of PubMed, EMBASE, Cochrane Library, and ClinicalTrials.gov was conducted. METHODS OF STUDY SELECTION: Four hundred thirty-three studies were screened for inclusion with 136 full-text articles reviewed...
December 4, 2017: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/29215351/moringa-oleifera-functionalised-sand-reuse-with-non-ionic-surfactant-dodecyl-glucoside
#15
Frances E Williams, Andrew K Lee, Sanaz Orandi, Sarah K Sims, David M Lewis
Moringa oleifera seeds are well known for their ability to cause flocculation in turbid water and facilitate bacterial inhibition. These effects are due to the cationic polypeptide MO2.1, which affects the surface charge of suspended particles and causes lysis of bacterial cells. However, the attachment of bacteria to MO2.1 prevents further bacterial attachment, reducing the effectiveness of the seeds. This research investigated the effect of surfactants on functionality and reuse of Moringa seeds to develop a sustainable water treatment technique...
October 2017: Journal of Water and Health
https://www.readbyqxmd.com/read/29214529/a-rapid-and-reagent-free-bioassay-for-the-detection-of-dioxin-like-compounds-and-other-aryl-hydrocarbon-receptor-ahr-agonists-using-autobioluminescent-yeast
#16
Tingting Xu, Anna Young, Enolia Marr, Gary Sayler, Steven Ripp, Dan Close
An autonomously bioluminescent Saccharomyces cerevisiae BLYAhS bioreporter was developed in this study for the simple and rapid detection of dioxin-like compounds (DLCs) and aryl hydrocarbon receptor (AhR) agonists. This recombinant yeast reporter was based on a synthetic bacterial luciferase reporter gene cassette (lux) that can produce the luciferase as well as the enzymes capable of self-synthesizing the requisite substrates for bioluminescent production from endogenous cellular metabolites. As a result, bioluminescent signal production is generated continuously and autonomously without cell lysis or exogenous reagent addition...
December 6, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29213245/implication-of-free-fatty-acids-in-thrombin-generation-and-fibrinolysis-in-vascular-inflammation-in-zucker-rats-and-evolution-with-aging
#17
Jérémy Lagrange, Mélusine Didelot, Amel Mohamadi, Lucy A Walton, Saartje Bloemen, Bas de Laat, Huguette Louis, Simon N Thornton, Brian Derby, Michael J Sherratt, Bruno Fève, Pascal Challande, Riaz Akhtar, J Kennedy Cruickshank, Patrick Lacolley, Véronique Regnault
Background: The metabolic syndrome (MetS) and aging are associated with modifications in blood coagulation factors, vascular inflammation, and increased risk of thrombosis. Objectives: Our aim was to determine concomitant changes in thrombin generation in the blood compartment and at the surface of vascular smooth muscle cells (VSMCs) and its interplay with adipokines, free fatty acids (FFA), and metalloproteinases (MMPs) in obese Zucker rats that share features of the human MetS. Methods: Obese and age-matched lean Zucker rats were compared at 25 and 80 weeks of age...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29212911/a-role-of-nkr-p1a-cd161-and-lectin-like-transcript-1-in-natural-cytotoxicity-against-human-articular-chondrocytes
#18
Agata Białoszewska, Joanna Olkowska-Truchanowicz, Katarzyna Bocian, Anna Osiecka-Iwan, Andrzej Czop, Claudine Kieda, Jacek Malejczyk
Normal cartilage cells are susceptible to lysis by NK cells. This phenomenon may play a role in immune cartilage destruction; however, the mechanisms of chondrocyte recognition by NK cells remain poorly understood. Therefore, the aim of this study was to reveal a possible role of NKR-P1A/lectin-like transcript 1 (LLT1) interaction in NK cell-mediated cytotoxicity against normal human articular chondrocytes. Chondrocytes were isolated from articular cartilage obtained during talonavicular joint surgery. PBMC or polyclonal NK cells isolated from normal donors served as effector cells...
December 6, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29212331/enhanced-antibacterial-activity-of-silver-ruthenium-coated-hollow-microparticles
#19
Alexander Heiss, Birger Freisinger, Evi Held-Föhn
The oxidation based antimicrobial activity of silver is long known. Microparticles with a particular silver-ruthenium coating and specific physical properties were developed. The coating showed a considerably increased silver ion release rate in comparison to a plain silver coating. Accordingly, an exposure of Escherichia coli and Staphylococcus aureus to these silver-ruthenium coated microparticles resulted in a time and concentration dependent cell killing. Even though contact killing may contribute to this efficacy, rather a release associated diffusion gradient dependent killing was observed...
December 5, 2017: Biointerphases
https://www.readbyqxmd.com/read/29210381/simple-and-rapid-detection-of-bacteria-using-a-nuclease-responsive-dna-probe
#20
Kyung Jin Lee, Wang Sik Lee, Ahreum Hwang, Jeong Moon, Taejoon Kang, Kyoungsook Park, Jinyoung Jeong
We demonstrate simple and rapid bacterial detection using a nuclease-responsive DNA probe. The probe consisting of a fluorescent dye and a quencher at the 5' and 3' termini, respectively, was designed to be cleaved by nucleases such as endonucleases, exonucleases, and DNases, which are released from bacteria using an optimized lysis buffer. The fluorescence signal of the cleaved DNA probe correlates with the number of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, and the detection limit was 103 CFU for E...
December 6, 2017: Analyst
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