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https://www.readbyqxmd.com/read/29045996/sicko-development-of-a-multi-disciplinary-training-concept-for-paediatric-oncology
#1
Petra Tiroke, Birte Sänger, Urs Mücke, Lea Berndt, Henrike Wittenbecher, Lorenz Grigull
Background Treating children with cancer requires multiple different skills. For the healthcare personnel (HCP) in Germany the practice of ongoing training to improve professional skills is almost non-existent. Therefore, we developed a programme called 'SICKO' to support HCPs skills and attitudes by means of a multidisciplinary workshop. Methods Following a qualitative analysis, we then designed a modular (3 day) workshop. During day one (8 h) participants learn practical skills, the fundamentals of chemotherapy, and effective communication skills...
October 18, 2017: Klinische Pädiatrie
https://www.readbyqxmd.com/read/29045759/the-effect-of-hydroxychloroquine-on-haemostasis-complement-inflammation-and-angiogenesis-in-patients-with-antiphospholipid-antibodies
#2
Karen Schreiber, Karen Breen, Kiran Parmar, Jacob H Rand, Xiao-Xuan Wu, Beverley J Hunt
Objectives: HCQ has been described as having a beneficial effect in patients with APS but its mechanism of action is unclear. We hypothesized that HCQ may have effects on subnormal angiogenesis, inflammation and haemostatic biomarkers seen in APS. The aim of our study was to assess laboratory markers [annexin A5 (AnxA5) anticoagulant activity, tissue factor (TF) levels, thromboelastography (TEG), CRP, Bb, C3a and VEGF] in HCQ-naïve patients with aPL at baseline and after commencing HCQ...
October 17, 2017: Rheumatology
https://www.readbyqxmd.com/read/29043672/preparation-of-highly-enriched-er-membranes-using-free-flow-electrophoresis
#3
Harriet T Parsons
Free-flow electrophoresis (FFE) is a technique for separation of proteins, peptides, organelles, and cells. With zone electrophoresis (ZE-FFE), organelles are separated according to surface charge. The ER is the only remaining major cellular compartment in Arabidopsis not to have been isolated using density centrifugation, immune-isolation, or any other method previously applied to purification of plant membranes. By using continuous-flow electrophoresis ER vesicles of similar surface charge, which may have been fragmented during cell lysis, can be focused...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29043349/comparative-suppressing-effects-of-black-and-green-teas-on-the-formation-of-advanced-glycation-end-products-ages-and-age-induced-oxidative-stress
#4
P Ramlagan, P Rondeau, C Planesse, V S Neergheen-Bhujun, E Bourdon, T Bahorun
This study aimed at investigating and comparing the anti-diabetic potential of black and green teas. Biochemical analyses indicate higher antioxidant potency, significantly correlated with the phytochemicals present, in green teas compared to black teas. Both extracts afforded a similar level of protection to erythrocytes against peroxyl radical-induced lysis. Non-cytotoxic concentration of green and black tea extracts significantly reduced the reactive oxygen species (ROS) production (P < 0.01), lowered the oxidation of proteins (P < 0...
October 18, 2017: Food & Function
https://www.readbyqxmd.com/read/29042500/immune-regulation-of-plasmodium-is-anopheles-species-specific-and-infection-intensity-dependent
#5
Maria L Simões, Godfree Mlambo, Abhai Tripathi, Yuemei Dong, George Dimopoulos
Malaria parasite ookinetes must traverse the vector mosquito midgut epithelium to transform into sporozoite-producing oocysts. The Anopheles innate immune system is a key regulator of this process, thereby determining vector competence and disease transmission. The role of Anopheles innate immunity factors as agonists or antagonists of malaria parasite infection has been previously determined using specific single Anopheles-Plasmodium species combinations. Here we show that the two C-type lectins CTL4 and CTLMA2 exert differential agonistic and antagonistic regulation of parasite killing in African and South American Anopheles species...
October 17, 2017: MBio
https://www.readbyqxmd.com/read/29042497/a-single-dual-function-enzyme-controls-the-production-of-inflammatory-nod-agonist-peptidoglycan-fragments-by-neisseria-gonorrhoeae
#6
Jonathan D Lenz, Kathleen T Hackett, Joseph P Dillard
Neisseria gonorrhoeae gonococcus (GC) is a Gram-negative betaproteobacterium and causative agent of the sexually transmitted infection gonorrhea. During growth, GC releases lipooligosaccharide (LOS) and peptidoglycan (PG) fragments, which contribute significantly to the inflammatory damage observed during human infection. In ascending infection of human Fallopian tubes, inflammation leads to increased risk of ectopic pregnancy, pelvic inflammatory disease, and sterility. Of the PG fragments released by GC, most are disaccharide peptide monomers, and of those, 80% have tripeptide stems despite the observation that tetrapeptide stems make up 80% of the assembled cell wall...
October 17, 2017: MBio
https://www.readbyqxmd.com/read/29039746/preclinical-testing-of-an-oncolytic-parvovirus-standard-protoparvovirus-h-1pv-efficiently-induces-osteosarcoma-cell-lysis-in-vitro
#7
Carsten Geiss, Zoltán Kis, Barbara Leuchs, Monika Frank-Stöhr, Jörg R Schlehofer, Jean Rommelaere, Christiane Dinsart, Jeannine Lacroix
Osteosarcoma is the most frequent malignant disease of the bone. On the basis of early clinical experience in the 1960s with H-1 protoparvovirus (H-1PV) in osteosarcoma patients, this effective oncolytic virus was selected for systematic preclinical testing on various osteosarcoma cell cultures. A panel of five human osteosarcoma cell lines (CAL 72, H-OS, MG-63, SaOS-2, U-2OS) was tested. Virus oncoselectivity was confirmed by infecting non-malignant human neonatal fibroblasts and osteoblasts used as culture models of non-transformed mesenchymal cells...
October 17, 2017: Viruses
https://www.readbyqxmd.com/read/29038827/is-routine-intraoperative-contrast-enhanced-ultrasonography-useful-during-whole-liver-transplantation
#8
Nicolas Golse, Simone Santoni, Vincent Karam, Oriana Ciacio, Gabriella Pittau, Marc-Antoine Allard, Daniel Cherqui, Antonio Sa Cunha, René Adam, Denis Castaing, Eric Vibert
BACKGROUND: Vascular complications following liver transplantation (LT) may result from technical deficiencies. Intraoperative diagnosis remains challenging but can prevent serious delayed complications. Intraoperative Doppler ultrasonography (IOUS) represents the gold standard for imaging, although it requires radiological skills. Contrast-enhanced ultrasonography has been reported during postoperative assessments, but never intraoperatively (CE-IOUS). The aim of this study was to assess the feasibility of routine CE-IOUS, to evaluate its impact on surgical strategy and its usefulness...
October 16, 2017: World Journal of Surgery
https://www.readbyqxmd.com/read/29035455/evaluation-of-a-magnetic-cellulose-based-dna-extraction-system-to-improve-the-performance-of-hybribio-human-papillomavirus-genotyping-and-screening-tests-for-cervical-swab-samples
#9
Zengge Wang, Yulin Zhou, Jun Ren, Zhongmin Xia, Qiwei Guo
BACKGROUND: To ensure the accuracy of clinical human papillomavirus (HPV) testing, the nucleic acid extraction procedure should be thoroughly evaluated for each clinical sample type. Therefore, we evaluated whether the MagCore® Automated Nucleic Acid Extraction system (MagCore system) could improve HybriBio HPV test performance for cervical swab samples. METHODS: We compared the performance of HybriBio HPV genotyping and screening tests using samples prepared with the MagCore system and Cell Lysis Kit, which was provided by the HPV test manufacturer...
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29032642/characterization-of-atpase-activity-of-free-and-immobilized-chromatophore-membrane-vesicles-of-rhodobacter-sphaeroides
#10
Hyeonjun Kim, Xiaomeng Tong, Sungyoung Choi, Jeong K Lee
The intracytoplasmic membrane of Rhodobacter sphaeroides readily vesiculates when cells are lysed. The resulting chromatophore membrane vesicle (CMV) contains the photosynthetic machineries to synthesize ATP by ATPase. The light-dependent ATPase activity of CMV was lowered in the presence of O₂, but the activity increased to the level observed under anaerobic condition when the reaction mixture was supplemented with ascorbic acid (> or =0.5 mM). Cell lysis in the presence of biotinyl cap phospholipid (bcp) resulted in the incorporation of bcp into membrane to form biotinylated CMV (bCMV), which binds to streptavidin resin at a ratio of approximately 24 μg bacteriochlorophyll a/ml resin...
October 14, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29032498/morphological-and-molecular-description-of-myxobolus-batalhensis-n-sp-myxozoa-myxosporea-a-liver-and-ovary-parasite-of-salminus-hilarii-in-brazil
#11
Diego Henrique Mirandola Dias Vieira, Gema Alama-Bermejo, Jerri Lee Bartholomew, Vanessa Doro Abdallah, Rodney Kozlowiski de Azevedo
Plasmodia containing myxospores belonging to the genus Myxobolus Bütschli, 1882 were found in the ovaries and liver of Salminus hilarii. Despite its economic value, this fish host has no previous reports of myxozoan infections. Herein, we describe Myxobolus batalhensis n. sp. using morphological and ultrastructural data, as well as histological and SSU rDNA molecular data. The mature myxospores were elongated, measuring in average 15.2 ± 0.8 μm in length, 8.4 ± 0.4 μm in width, and 5.1 ± 0.2 μm in thickness...
October 14, 2017: Parasitology Research
https://www.readbyqxmd.com/read/29030485/crosstalk-between-pka-and-pkg-controls-ph-dependent-host-cell-egress-of-toxoplasma-gondii
#12
Yonggen Jia, Jean-Baptiste Marq, Hugo Bisio, Damien Jacot, Christina Mueller, Lu Yu, Jyoti Choudhary, Mathieu Brochet, Dominique Soldati-Favre
Toxoplasma gondii encodes three protein kinase A catalytic (PKAc1-3) and one regulatory (PKAr) subunits to integrate cAMP-dependent signals. Here, we show that inactive PKAc1 is maintained at the parasite pellicle by interacting with acylated PKAr. Either a conditional knockdown of PKAr or the overexpression of PKAc1 blocks parasite division. Conversely, down-regulation of PKAc1 or stabilisation of a dominant-negative PKAr isoform that does not bind cAMP triggers premature parasite egress from infected cells followed by serial invasion attempts leading to host cell lysis...
October 13, 2017: EMBO Journal
https://www.readbyqxmd.com/read/29029468/resveratrol-promotes-mica-b-expression-and-natural-killer-cell-lysis-of-breast-cancer-cells-by-suppressing-c-myc-mir-17-pathway
#13
Jie Pan, Jiaying Shen, Wengong Si, Chengyong Du, Danni Chen, Liang Xu, Minya Yao, Peifen Fu, Weimin Fan
Major histocompatibility complex class I chain-related proteins A and B (MICA and MICB) are important ligands for recognition of tumor cells by immune effector cells. Here, we report that resveratrol upregulated the protein and mRNA expression of MICA and MICB in breast cancer cells, which in turn promoted breast cancer cell lysis by natural killer (NK) cells in vitro and in vivo. Antibodies against NK group 2 member D blocked this effect. The 3'-untranslated regions of MICA and MICB were found to be direct binding targets of miR-17...
September 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29026928/-current-treatment-concepts-in-intracerebral-hemorrhage
#14
REVIEW
H B Huttner, J B Kuramatsu
BACKGROUND AND OBJECTIVE: In recent years, various important studies investigating the management of intracerebral hemorrhage (ICH) have been published. However, these have not entered guideline recommendations yet. Therefore, essential results are summarized here and the findings are integrated into current treatment concepts. MATERIALS AND METHODS: Based on a dedicated literature review and the authors' experience, up-to-date and high-quality investigations were identified...
October 12, 2017: Medizinische Klinik, Intensivmedizin und Notfallmedizin
https://www.readbyqxmd.com/read/29025561/arterial-thrombus-stability-does-it-matter-and-can-we-detect-it
#15
REVIEW
Diana A Gorog, Zahi A Fayad, Valentin Fuster
The spontaneous lysis of a coronary thrombus is a natural protective mechanism against lasting occlusion and downstream infarction. Thrombus stability is thus a direct determinant of clinical outcome. Compared with the extensive study of the crucial role of platelets, coagulation, and flow in arterial thrombosis, little attention has been paid to factors affecting thrombus stability, despite evidence linking impaired spontaneous fibrinolytic activity with acute coronary events. We summarize experimental evidence for the importance of thrombus stability and highlight the need for physiologically relevant tests to assess spontaneous disintegration/fibrinolysis of platelet-rich thrombi under arterial flow conditions, review techniques to assess thrombus stability in vitro, and describe novel imaging techniques to characterize thrombosis in vivo...
October 17, 2017: Journal of the American College of Cardiology
https://www.readbyqxmd.com/read/29024798/hysteroscopic-management-of-asherman-s-syndrome-special-issue-report-on-uterine-surgery-in-the-journal-of-minimally-invasive-gynecology
#16
Zaraq Khan, Jeffrey M Goldberg
In developed countries, Asherman's Syndrome is almost always the result of a prior intrauterine operative trauma. This is often asymptomatic but may result in hypo- or amenorrhea and can contribute to infertility and pregnancy complications. We will review their etiology, clinical implications, and the systems which have been proposed to classify their extent. The numerous methods reported for performing lysis of intrauterine adhesions will be summarized along with the clinical results. Current strategies to prevent recurrence of intrauterine adhesions have not been conclusively shown to be clinically effective but the potential for endometrial regeneration using stem cells is an exciting modality under investigation...
October 9, 2017: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/29021675/surgical-treatment-of-bilateral-nondisplaced-isthmic-lysis-by-interlaminar-fixation-device
#17
Keyvan Mostofi, Reza Karimi Khouzani
STUDY DESIGN: Spondylolysis is a defect in the portion of pars interarticularis. The latter affects approximately 6% of the population. It is caused by repetitive trauma in hyperextension. Low back pain is the most common symptom. METHODS: We implanted interspinous process devices in 12 patients with isthmic lysis without spondylolisthesis for low back pain. The purpose of the surgery was to conduct a minimally invasive procedure. RESULTS: In eight cases, patients became asymptomatic...
July 2017: Journal of Craniovertebral Junction and Spine
https://www.readbyqxmd.com/read/29020506/potential-application-of-immobilized-streptokinase-extracted-from-streptococcus-equinus-vit_vb2
#18
B Vaishnavi, C Subathra Devi
Streptokinase purified from Streptococcus equinus VIT_VB2 isolated from bovine milk sample was immobilized in various solid supports namely entrapment in agarose gel, calcium alginate beads and gelatin gel by crosslinking with formaldehyde. Immobilization of streptokinase in calcium alginate beads showed maximum efficiency (81.8 ± 1.06%) when compared to entrapment with agarose gel (55.6 ± 2.17%) and cross-linked gelatin formaldehyde gel (71.0 ± 1.54%). The purified SK activity was expressed maximum in calcium alginate (1%) and gelatin gel (0...
October 11, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/29019678/total-synthesis-and-biological-mode-of-action-of-wap-8294a2-a-menaquinone-targeting-antibiotic
#19
Hiroaki Itoh, Kotaro Tokumoto, Takuya Kaji, Atmika Paudel, Suresh Panthee, Hiroshi Hamamoto, Kazuhisa Sekimizu, Masayuki Inoue
WAP-8294A2 (lotilibcin, 1) is a potent antibiotic with superior in vivo efficacy to vancomycin against methicillin-resistant Staphylococcus aureus (MRSA). Despite the great medical importance, its molecular mode of action remains unknown. Here we report the total synthesis of complex macrocyclic peptide 1 comprised of 12 amino acids with a β-hydroxy fatty-acid chain, and its deoxy analogue 2. A full solid-phase synthesis of 1 and 2 enabled their rapid assembly and the first detailed investigation of their functions...
October 11, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/29018424/sample-preservation-dna-or-rna-extraction-and-data-analysis-for-high-throughput-phytoplankton-community-sequencing
#20
Anita Mäki, Pauliina Salmi, Anu Mikkonen, Anke Kremp, Marja Tiirola
Phytoplankton is the basis for aquatic food webs and mirrors the water quality. Conventionally, phytoplankton analysis has been done using time consuming and partly subjective microscopic observations, but next generation sequencing (NGS) technologies provide promising potential for rapid automated examination of environmental samples. Because many phytoplankton species have tough cell walls, methods for cell lysis and DNA or RNA isolation need to be efficient to allow unbiased nucleic acid retrieval. Here, we analyzed how two phytoplankton preservation methods, three commercial DNA extraction kits and their improvements, three RNA extraction methods, and two data analysis procedures affected the results of the NGS analysis...
2017: Frontiers in Microbiology
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