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Sheep bronchoscopy

Victoria K Pepper, Ekene A Onwuka, Cameron A Best, Nakesha King, Eric Heuer, Jed Johnson, Christopher K Breuer, Jonathan M Grischkan, Tendy Chiang
OBJECTIVE: To evaluate the safety and efficacy of bronchoscopic interventions in the management of tissue-engineered tracheal graft (TETG) stenosis. STUDY DESIGN: Animal research study. METHODS: TETGs were constructed with seeded autologous bone marrow-derived mononuclear cells on a bioartificial graft. Eight sheep underwent tracheal resection and orthotopic implantation of this construct. Animals were monitored by bronchoscopy and fluoroscopy at 3 weeks, 6 weeks, 3 months, and 4 months...
October 2017: Laryngoscope
Lauren E McClain, Marcus G Davey, Phillip W Zoltick, Maria P Limberis, Alan W Flake, William H Peranteau
PURPOSE: Successful in utero or perinatal gene therapy for congenital lung diseases, such as cystic fibrosis and surfactant protein deficiency, requires identifying clinically relevant viral vectors that efficiently transduce airway epithelial cells. The purpose of the current preclinical large animal study was to evaluate lung epithelium transduction of adeno-associated viral (AAV) vector serotypes following intratracheal delivery. METHODS: Six different AAV vector serotypes (AAV1, AAV5, AAV6, AAV8, AAV9, and AAVrh10) expressing the green fluorescent protein (GFP) as the transgene were injected into the right upper lobe of perinatal sheep via bronchoscopy...
June 2016: Journal of Pediatric Surgery
Tamer Y El Mays, Parichita Choudhury, Richard Leigh, Emmanuel Koumoundouros, Joanne Van der Velden, Grishma Shrestha, Cora A Pieron, John H Dennis, Francis Hy Green, Ken J Snibson
BACKGROUND: The low toxicity of perfluorocarbons (PFCs), their high affinity for respiratory gases and their compatibility with lung surfactant have made them useful candidates for treating respiratory diseases such as adult respiratory distress syndrome. We report results for treating acute allergic and non-allergic bronchoconstriction in sheep using S-1226 (a gas mixture containing carbon dioxide and small volumes of nebulized perflubron). The carbon dioxide, which is highly soluble in perflubron, was used to relax airway smooth muscle...
2014: Respiratory Research
Lidek Chou, Andriy Batchinsky, Slava Belenkiy, Joseph Jing, Tirunelveli Ramalingam, Matthew Brenner, Zhongping Chen
We report on the feasibility of using long-range swept-source optical coherence tomography (OCT) to detect airway changes following smoke inhalation in a sheep model. The long-range OCT system (with axial imaging range of 25 mm) and probe are capable of rapidly obtaining a series of high-resolution full cross-sectional images and three-dimensional reconstructions covering 20-cm length of tracheal and bronchial airways with airway diameter up to 25 mm, regardless of the position of the probe within the airway lumen...
March 2014: Journal of Biomedical Optics
David Collie, John Govan, Steven Wright, Elisabeth Thornton, Peter Tennant, Sionagh Smith, Catherine Doherty, Gerry McLachlan
BACKGROUND: Chronic lung infection with Pseudomonas aeruginosa is a major contributor to morbidity, mortality and premature death in cystic fibrosis. A new paradigm for managing such infections is needed, as are relevant and translatable animal models to identify and test concepts. We sought to improve on limitations associated with existing models of infection in small animals through developing a lung segmental model of chronic Pseudomonas infection in sheep. METHODOLOGY/PRINCIPAL FINDINGS: Using local lung instillation of P...
2013: PloS One
Chong Bai, Yu-chao Dong, Xiao-lian Song, Yi Huang, Hui Shi, Zhen-li Hu, Qiang Li
BACKGROUND: The usual transbronchial coagulation techniques include microwave, argon plasma coagulation (APC), electrocautery and cryotherapy. However, there are serious clinical problems in the safety of each. By analyzing the experimental data and clinical observations, we observed the variable effects of different coagulation techniques via bronchofibroscopy, to look for an optimal interventional management of luminal bronchus diseases, and evaluate the safety and the equivalent point...
January 2013: Chinese Medical Journal
Aurélie Maria Derweduwen, Erik Verbeken, Marguerite Stas, Johny Verschakelen, Johan Coolen, Geert Verleden, Wim Wuyts
We present a case of a 32-year-old woman who was diagnosed with lymphangioleiomyomatosis (LAM) after detecting a mass in the upper anterior mediastinum. Two years after presentation another metastatic localisation of LAM occurred in the cervical region. With this article we would like to highlight the fact that there are still a lot of unanswered questions, especially regarding the best management of extrapulmonary LAM.
January 2013: Thorax
Saeid Amanpour, Hamidreza Abtahi, Shahram Rabbani, Samad Muhammadnezhad
PURPOSE: Although bronchoscopy can be safely performed through endotracheal tube in most intubated critically ill patients, sometimes it could lead to complications such as hypoxia and high airway pressures. Theoretically, transglottic bronchoscopy (TGB) does not interfere with mechanical ventilation and could avoid these complications. In a two-period crossover study, we compared this technique with trans-endotracheal tube bronchoscopy (TEB) in normal anesthetized sheep. METHODS: In five sheep, we did TGB first...
August 2012: Journal of Anesthesia
Hisashi Tsukada, Sidhu Gangadharan, Robert Garland, Felix Herth, Malcolm DeCamp, Armin Ernst
BACKGROUND: A significant native tracheal approximation phenomenon was observed in our previous study [Tsukada H, Ernst A, Gangadharan S, et al. Tracheal replacement with a silicone-stented fresh aortic allograft in sheep. Ann Thorac Surg 2010;89:253-8], in which sheep trachea was replaced with an allogenic aortic graft in order to attempt transplantation. Because an appropriate tracheal replacement graft has yet to be determined, other means to repair or replace tracheal tissue have to be evaluated...
December 2010: Annals of Thoracic Surgery
Hisashi Tsukada, Carl R O'Donnell, Robert Garland, Felix Herth, Malcolm Decamp, Armin Ernst
OBJECTIVE: Tracheobronchomalacia (TBM) is increasingly recognized as a condition associated with significant pulmonary morbidity. However, treatment is invasive and complex, and because there is no appropriate animal model, novel diagnostic and treatment strategies are difficult to evaluate. We endeavored to develop a reliable airway model to simulate hyperdynamic airway collapse in humans. METHODS: Seven 20-kg male sheep were enrolled in this study. Tracheomalacia was created by submucosal resection of > 50% of the circumference of 10 consecutive cervical tracheal cartilage rings through a midline cervical incision...
December 2010: Chest
Hisashi Tsukada, Armin Ernst, Sidhu Gangadharan, Simon Ashiku, Robert Garland, Diana Litmanovich, Malcolm DeCamp
BACKGROUND: Tracheal tissue regeneration after allogeneic aortic transplants in sheep has been reported. We sought to confirm these findings and elucidate the mechanism of this transformation. METHODS: Ten male sheep underwent cervical tracheal replacement with fresh, descending thoracic aortic allografts, 8 cm long, from female sheep, without postoperative immunosuppressive therapy. A 10-cm silicone stent was placed to prevent airway collapse. Graft evaluations with flexible bronchoscopy and computed tomography were conducted between 2 weeks and 1 year after surgery...
January 2010: Annals of Thoracic Surgery
Tsuyoshi Tanabe, Tomonobu Koizumi, Kenji Tsushima, Michiko Ito, Shintaro Kanda, Takashi Kobayashi, Masanori Yasuo, Yoshitaka Yamazaki, Keishi Kubo, Takayuki Honda, Ryouichi Kondo, Kazuo Yoshida
BACKGROUND: We previously reported that bronchoscopy-guided, internally cooled radiofrequency ablation (RFA) in normal sheep lung was a safe, effective, and feasible procedure without major complications. PURPOSES: The aim of this study was to evaluate the safety, effectiveness, and feasible conditions of bronchoscopy-guided, internally cooled RFA as a clinical application for non-small cell lung cancer (NSCLC). METHODS: Ten patients pathologically diagnosed with NSCLC and the clinical stage of T1N0M0 were enrolled in the study...
April 2010: Chest
Hung-Hsun Yen, Janet L-K Wee, Kenneth J Snibson, Jean-Pierre Y Scheerlinck
We have developed and validated a novel method to access efferent lymph draining the lung and gut of sheep. In this model, efferent lymph derived from the lung could be collected via cannulation of the thoracic duct just prior the thoracic duct-jugular vein junction. The thoracic duct was accessed in the neck region without needing to broach the thoracic cavity, thus avoiding extensive tissue damage to the animal and need for ventilation during surgery. In addition, this surgical approach allows for a second cannulation of an adjacent lymphatic draining the head/neck region, providing for an 'in-built' internal control with which to compare lymph parameters...
May 15, 2009: Veterinary Immunology and Immunopathology
Matthias Behrend, Eva Kluge
OBJECTIVE: An established method of tracheal substitution is not yet available, but homograft tracheal transplantation might provide a realistic tracheal replacement. With the objective of sequentially examining the healing of tracheal homografts, we have established a suitable large-animal model. METHODS: Five sheep received orthotopic tracheal transplantation of a 4-cm cervical tracheal homograft. The trachea was supported for 6 weeks with a self-expanding polyester stent...
August 2008: World Journal of Surgery
David Feller-Kopman, Jeanne M Lukanich, Gil Shapira, Uri Kolodny, Baruch Schori, Heather Edenfield, Burak Temelkuran, Armin Ernst, Yair Schindel, Yoel Fink, Jon Fox, Raphael Bueno
BACKGROUND: Thermal ablation is one of the most commonly used modalities to treat central airway obstruction. Both laser and argon plasma coagulation (APC) have been reported to cause gas emboli and cardiac arrest. We sought to determine whether bronchoscopic ablation therapy can result in systemic gas emboli, correlate their presence with the rate of gas flow, and establish whether a zero-flow (ZF) modality would result in the significant reduction or elimination of emboli. METHODS: CO(2) laser delivered through a photonic bandgap fiber (PBF) and APC were applied in the trachea and mainstem bronchi of six anesthetized sheep at varying dosages and gas flow rates...
April 2008: Chest
Marc Noppen
No abstract text is available yet for this article.
2007: Respiration; International Review of Thoracic Diseases
Larry W Tsai, Andrew M Hoffman, Melissa R Mazan, Edward P Ingenito
BACKGROUND: The development of bronchoscopic therapies for emphysema has renewed interest in collateral ventilation. The success or failure of bronchoscopically placed valves or biologic glues may be determined by collateral ventilation, which is exaggerated in emphysema. Furthermore, the validity of various animal models of emphysema for testing such techniques must be understood in the context of their species-specific collateral ventilation. OBJECTIVES: To quantify collateral ventilation in a sheep model of emphysema using a simple in vivo bronchoscopic method...
2007: Respiration; International Review of Thoracic Diseases
John Reilly, George Washko, Victor Pinto-Plata, Eduardo Velez, Lawrence Kenney, Robert Berger, Bartolome Celli
BACKGROUND: Biological lung volume reduction (BLVR) using biological reagents to remodel and shrink damaged regions of lung has previously been accomplished in sheep with experimental pulmonary emphysema. This report summarizes the initial clinical experience including a 3-month follow-up using this technique in humans. METHODS: An open-label phase 1 trial designed to evaluate the safety of BLVR in patients with advanced heterogeneous emphysema enrolled six patients...
April 2007: Chest
K Tsushima, T Koizumi, T Tanabe, R Nakagawa, S Yoshikawa, M Yasuo, K Kubo
The aim of the current study was to assess the safety of bronchoscopy-guided radiofrequency ablation (RFA) and compare the effectiveness between new internal cooled-RFA and standard noncooled-RFA. Normal lungs from sheep were used (n=6). Internal cooled-RFA and standard noncooled-RFA were set to assess the most suitable RFA conditions, such as power output, flow rate and ablation time. Internal cooled-RFA was then applied under the most optimal conditions of power output and flow rate for 15, 30, 60 and 120 s, and two water temperatures either room temperature (RT) water or cold water...
June 2007: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
Qi Wu, Jun-ping Wu, Yong Fan, Chun-bao Liang, Jing-liang Ma, Ping Li, Jing-na Hua, Yong-mei Wang, Jin-rong Wang, Wei-ping Li, Bao-xiang Ren
OBJECTIVE: To evaluate the effectiveness of bronchoscopic lung volume reduction (BLVR) using one way flap device in sheep model of heterogeneous emphysema. METHODS: Six 6-month sheep (weight: 20-30 kg) were treated with localized papain instillations to generate heterogeneous emphysema, subsequently underwent BLVR using one way-flap device at subsegment. Lung functional residual capacity (FRC) was analyzed before and 8 weeks after operation. Animals were euthanized at the 8-week time point...
August 2006: Zhongguo Wei Zhong Bing Ji Jiu Yi Xue, Chinese Critical Care Medicine, Zhongguo Weizhongbing Jijiuyixue
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