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https://www.readbyqxmd.com/read/28088615/cloning-heterologous-expression-and-biochemical-characterization-of-a-non-specific-endoglucanase-family-12-from-aspergillus-terreus-nih2624
#1
Fernando Segato, Bruno Dias, Gabriela L Berto, Dyoni M de Oliveira, Flávio H M De Souza, Matheus Augusto R N da Silva, Ana Paula Citadini, Mario T Murakami, André R L Damásio, Fábio Márcio Squina, Igor Polikarpov
The cellulases from Glycoside Hydrolyses family 12 (GH12) play an important role in cellulose degradation and plant cell wall deconstruction being widely used in a number of bioindustrial processes. Aiming to contribute toward better comprehension of these class of the enzymes, here we describe a high-yield secretion of a endoglucanase GH12 from Aspegillus terreus (AtGH12), which was cloned and expressed in Aspergillus nidulans strain A773. The purified protein was used for complete biochemical and functional characterization...
January 11, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28087297/inhibition-of-streptococcal-biofilm-by-hydrogen-water
#2
Jinkyung Kim, Heon-Jin Lee, Su-Hyung Hong
OBJECTIVES: The accumulation of oral bacterial biofilm is the main etiological factor of oral diseases. Recently, electrolyzed hydrogen-rich water (H-water) has been shown to act as an effective antioxidant by reducing oxidative stress. In addition to this general health benefit, H-water has antibacterial activity for disease-associated oral bacteria. However, little is known about the effect of H-water on oral bacterial biofilm. The objective of this study was to confirm the effect of H-water on streptococcal biofilm formation...
January 10, 2017: Journal of Dentistry
https://www.readbyqxmd.com/read/28081524/effects-of-acidified-aqueous-glycerol-and-glycerol-carbonate-pretreatment-of-rice-husk-on-the-enzymatic-digestibility-structural-characteristics-and-bioethanol-production
#3
Majid Ebrahimi, Oliver B Villaflores, Emma E Ordono, Alvin R Caparanga
Rice husk as an abundant biomass was used in this study, and it contained 30.1% glucan and 13.5% xylan, 22.4% lignin. The pretreated rice husk with glycerol carbonate and acidified aqueous glycerol (10% water) at 90°C and 130°C for 60min had the maximum yield of glucan digestibility which was 78.2% and 69.7% respectively, using cellulase for 72h. The simultaneous saccharification and fermentation was conducted anaerobically at 37°C with Saccharomyces cerevisiae, 5% w/v glucan and 10FPU/g glucan of cellulase...
January 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28078651/fed-batch-enzymatic-saccharification-of-high-solids-pretreated-lignocellulose-for-obtaining-high-titers-and-high-yields-of-glucose
#4
Young Hoon Jung, Hyun Min Park, Dong Hyun Kim, Jungwoo Yang, Kyoung Heon Kim
To reduce the distillation costs of cellulosic ethanol, it is necessary to produce high sugar titers in the enzymatic saccharification step. To obtain high sugar titers, high biomass loadings of lignocellulose are necessary. In this study, to overcome the low saccharification yields and the low operability of high biomass loadings, a fed-batch saccharification process was developed using an enzyme reactor that was designed and built in-house. After optimizing the cellulase and biomass feeding profiles and the agitation speed, 132...
January 11, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28078259/effects-of-blends-of-low-protein-winter-wheat-flour-and-barley-byproducts-on-quality-changes-in-noodles
#5
Na-Young Lee
The physicochemical characteristics of fresh noodles made with blends of low-protein wheat flour and barley byproduct (BBP, 250 μm) were investigated. The crude protein contents (PC) of flour from Goso and Backjoong cultivars were 7.91% and 7.67%, respectively. PC and β-glucan contents from the BBP were 14.10% and 3.11%, respectively, which were higher than those in wheat flour. The water-holding capacity (WHC) of various blends was increased as a function of BBP but not gluten contents. Goso flour had the highest starch content (78...
December 2016: Preventive Nutrition and Food Science
https://www.readbyqxmd.com/read/28071024/effect-of-barley-%C3%AE-glucan-addition-as-a-fat-replacer-on-muffin-quality
#6
Sylwia Onacik-Gür, Anna Żbikowska, Ewa Kapler, Hanna Kowalska
BACKGROUND: The aim of this study was to perform the partial replacement of bakery fat with barley β-glucan in muffins and to determine its effect on the physical properties of products. Most shortenings used in the industry are solid fats rich in saturated fatty acids and often trans fatty isomers, which are nutritionally unfavorable. METHODS: Dough and baked muffins were used as the research material. Five muffin recipes were prepared: control (K0%) with 16% fat content in the total dough weight, with fat content decreased by 10% (PG10%), 15% (PG15%), 20% (PG20%) and 25% (PG25%)...
July 2016: Acta Scientiarum Polonorum. Technologia Alimentaria
https://www.readbyqxmd.com/read/28069658/scy-078-is-fungicidal-in-time-kill-studies-against-candida-species
#7
Bernard Scorneaux, David Angulo, Katyna Borroto-Esoda, Mahmoud Ghannoum, Michael Peel, Stephen Wring
SCY-078 is an orally bioavailable ß-1,3-glucan synthesis inhibitor (GSI) and the first-in-class of structurally novel triterpine antifungals in clinical development for treating candidemia and invasive candidiasis. In vitro susceptibility by broth micro-dilution, antifungal carry-over, and time-kill dynamics were determined for 3 reference (ATCC) strains (C. albicans 90028, C. parapsilosis 90018, and C. tropicalis 750), a Quality Control (QC) strain (C krusei 6258), and 4 other strains (C. albicans MYA-2732, 64124, 76485 and C...
January 9, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28069433/dual-recognition-activity-of-a-rhamnose-binding-lectin-to-pathogenic-bacteria-and-zooxanthellae-in-stony-coral-pocillopora-damicornis
#8
Zhi Zhou, Xiaopeng Yu, Jia Tang, Yunjie Zhu, Guangmei Chen, Liping Guo, Bo Huang
Rhamnose-binding lectin (RBL) is a type of Ca(2+)-independent lectin with tandem repeat carbohydrate-recognition domain, and is crucial for the innate immunity in many invertebrates. In this study, the cDNA sequence encoding RBL in coral Pocillopora damicornis (PdRBL-1) was cloned. The PdRBL-1 protein shared highest amino acid sequence similarity (55%) with the polyp of Hydra vulgaris, and contained a signal peptide and two tandem carbohydrate-recognition domains in which all cysteine residues were conserved...
January 6, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28067025/whole-grain-oats-more-than-just-a-fiber-role-of-unique-phytochemicals
#9
REVIEW
Shengmin Sang, YiFang Chu
Oats are a good source of soluble dietary fiber, especially β-glucan, which has outstanding functional and nutritional properties. β-Glucan is considered to be the major active component of oats because of its cholesterol-lowering and anti-diabetic effects. However, the nutritional benefits of oats appear to go beyond fiber to bioactive phytochemicals with strong antioxidant and anti-inflammatory effects. In this review, we summarize current knowledge on the chemistry, stability, bioavailability, and health effects of two unique phytochemicals in oats, avenanthramides (AVAs) and avenacosides A and B...
January 9, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28065507/crystal-structure-of-4-6-%C3%AE-glucanotransferase-supports-diet-driven-evolution-of-gh70-enzymes-from-%C3%AE-amylases-in-oral-bacteria
#10
Yuxiang Bai, Joana Gangoiti, Bauke W Dijkstra, Lubbert Dijkhuizen, Tjaard Pijning
Food processing and refining has dramatically changed the human diet, but little is known about whether this affected the evolution of enzymes in human microbiota. We present evidence that glycoside hydrolase family 70 (GH70) glucansucrases from lactobacilli, synthesizing α-glucan-type extracellular polysaccharides from sucrose, likely evolved from GH13 starch-acting α-amylases, via GH70 4,6-α-glucanotransferases. The crystal structure of a 4,6-α-glucanotransferase explains the mode of action and unique product specificity of these enzymes...
December 21, 2016: Structure
https://www.readbyqxmd.com/read/28060571/m-ficolin-is-present-in-aspergillus-fumigatus-infected-lung-and-modulates-epithelial-cell-immune-responses-elicited-by-fungal-cell-wall-polysaccharides
#11
Kasper Jensen, Kit P Lund, Kimmie B Christensen, Anne T Holm, Lalit Kumar Dubey, Jesper B Moeller, Christine S Jepsen, Anders Schlosser, László Galgóczy, Steffen Thiel, Uffe Holmskov, Grith L Sorensen
Aspergillus fumigatus (A. fumigatus) is an opportunistic fungal pathogen that typically infects the lungs of immunocompromised patients. Ficolins are secreted pattern recognition molecules of the innate immune system. In the present study, we investigated the unknown role of human M-ficolin in the recognition of, and response to fungal cell wall polysaccharides. M-ficolin was localized to infiltrating granulocytes and monocytes found in the A. fumigatus infected lung. Recombinant M-ficolin (rM-ficolin) bound specifically to A...
January 6, 2017: Virulence
https://www.readbyqxmd.com/read/28059108/4-3-%C3%AE-glucanotransferase-a-novel-reaction-specificity-in-glycoside-hydrolase-family-70-and-clan-gh-h
#12
Joana Gangoiti, Sander S van Leeuwen, Gerrit J Gerwig, Stéphane Duboux, Christina Vafiadi, Tjaard Pijning, Lubbert Dijkhuizen
Lactic acid bacteria possess a diversity of glucansucrase (GS) enzymes that belong to glycoside hydrolase family 70 (GH70) and convert sucrose into α-glucan polysaccharides with (α1 → 2)-, (α1 → 3)-, (α1 → 4)- and/or (α1 → 6)-glycosidic bonds. In recent years 3 novel subfamilies of GH70 enzymes, inactive on sucrose but using maltodextrins/starch as substrates, have been established (e.g. GtfB of Lactobacillus reuteri 121). Compared to the broad linkage specificity found in GSs, all GH70 starch-acting enzymes characterized so far possess 4,6-α-glucanotransferase activity, cleaving (α1 → 4)-linkages and synthesizing new (α1 → 6)-linkages...
January 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28056105/the-impact-of-microrna-223-3p-on-il-17-receptor-d-expression-in-synovial-cells
#13
Nozomu Moriya, Seiji Shibasaki, Miki Karasaki, Tsuyoshi Iwasaki
OBJECTIVE: Rheumatoid arthritis (RA) is an autoimmune inflammatory disease affecting joints. Elevated plasma levels of microRNA-223-3p (miR-223-3p) in patients with RA are implicated in the pathogenesis of the disease. This study aimed to analyze the functional role of miR-223-3p in the pathogenesis of RA by overexpressing miR-223-3p in synovial cell lines. METHODS: Arthritis was induced in the RA model of SKG mice by injection of ß-glucan. The histopathologic features of joints were examined using hematoxylin and eosin and immunohistochemical staining...
2017: PloS One
https://www.readbyqxmd.com/read/28054934/evaluation-of-the-potential-of-chitosan-%C3%AE-1-3-glucan-ha-material-as-scaffold-for-living-bone-graft-production-i-in-vitro-i-by-comparison-of-adsc-and-bmdsc-behaviour-on-its-surface
#14
Agata Przekora, Marta Vandrovcova, Martina Travnickova, Julia Pajorova, Martin Molitor, Grazyna Ginalska, Lucie Bacakova
The opinion regarding the origin of adult stem cells that should be used for living bone construct generation is strongly divided in the scientific community. In this study, the potential of chitosan/β-1,3-glucan/HA material as scaffold for bone regeneration applications was evaluated by behaviour comparison of adult stem cells derived from both origins - adipose tissue (ADSCs) and bone marrow (BMDSCs). In the case of ADSC isolation, low and high negative pressures were applied during liposuction procedure in order to determine if negative pressure settings may have impact on subsequent cell behaviour <i>in vitro</i>...
January 5, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28054497/the-lipopolysaccharide-lipid-a-long-chain-fatty-acid-is-important-for-rhizobium-leguminosarum-growth-and-stress-adaptation-in-free-living-and-nodule-environments
#15
Dianna V Bourassa, Elmar Kannenberg, Janine Sherrier, Richard Jeffrey Buhr, Russell Carlson
Rhizobium bacteria live in soil and plant environments, are capable of inducing symbiotic nodules on legumes, invade these nodules, and develop into bacteroids that fix atmospheric nitrogen into ammonia. Rhizobial lipopolysaccharide (LPS) is anchored in the bacterial outer membrane through a specialized lipid A containing a very long chain fatty acid (VLCFA). VLCFA function for rhizobial growth in soil and plant environments is not well understood. Two genes, acpXL and lpxXL, encoding acyl carrier protein and acyltransferase, are among the six genes required for biosynthesis and transfer of VLCFA to lipid A...
January 5, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28053668/study-of-traits-and-recalcitrance-reduction-of-field-grown-comt-down-regulated-switchgrass
#16
Mi Li, Yunqiao Pu, Chang Geun Yoo, Erica Gjersing, Stephen R Decker, Crissa Doeppke, Todd Shollenberger, Timothy J Tschaplinski, Nancy L Engle, Robert W Sykes, Mark F Davis, Holly L Baxter, Mitra Mazarei, Chunxiang Fu, Richard A Dixon, Zeng-Yu Wang, C Neal Stewart, Arthur J Ragauskas
BACKGROUND: The native recalcitrance of plants hinders the biomass conversion process using current biorefinery techniques. Down-regulation of the caffeic acid O-methyltransferase (COMT) gene in the lignin biosynthesis pathway of switchgrass reduced the thermochemical and biochemical conversion recalcitrance of biomass. Due to potential environmental influences on lignin biosynthesis and deposition, studying the consequences of physicochemical changes in field-grown plants without pretreatment is essential to evaluate the performance of lignin-altered plants...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28046010/tetr-family-regulator-brpt-modulates-biofilm-formation-in-streptococcus-sanguinis
#17
Jinlin Liu, Victoria N Stone, Xiuchun Ge, Madison Tang, Fadi Elrami, Ping Xu
Biofilms are a key component in bacterial communities providing protection and contributing to infectious diseases. However, mechanisms involved in S. sanguinis biofilm formation have not been clearly elucidated. Here, we report the identification of a novel S. sanguinis TetR repressor, brpT (Biofilm Regulatory Protein TetR), involved in biofilm formation. Deletion of brpT resulted in a significant increase in biofilm formation. Interestingly, the mutant accumulated more water soluble and water insoluble glucans in its biofilm compared to the wild-type and the complemented mutant...
2017: PloS One
https://www.readbyqxmd.com/read/28041553/characterisations-of-lactobacillus-reuteri-sk24-003-glucansucrase-implications-for-%C3%AE-gluco-poly-and-oligosaccharides-biosynthesis
#18
Ming Miao, Yajun Ma, Bo Jiang, Steve W Cui, Zhengyu Jin, Tao Zhang
Glucansucrase was obtained from Lactobacillus reuteri SK24.003 and its characterizations and in vitro biosynthesis for glucose polymer and oligosaccharides were investigated. The final specific activity of glucansucrase was 1.3IU/mg protein with 8.6-fold purification and 8.7% recovery. The molecular weight of purified enzyme was 166.0kDa. The glucansucrase exhibited optimum activity at 30-35°C, whereas the maximum activity was obtained at pH 5.0-5.5. The double-charged ions including Mg(2+), Mn(2+), Ni(2+), Co(2+), Ca(2+), Fe(2+) and Zn(2+) activated the glucansucrase activities and Ca(2+) ion highly stimulated the activity by approximately 4 times...
May 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28035976/meadowsweet-teas-as-new-functional-beverages-comparative-analysis-of-nutrients-phytochemicals-and-biological-effects-of-four-filipendula-species
#19
Daniil N Olennikov, Nina I Kashchenko, Nadezhda K Chirikova
In recent years, the increased popularity of functional beverages such as herbal teas and decoctions has led to the search for new sources of raw materials that provide appropriate taste and functionality to consumers. The objective of this study was to investigate the nutritional, phytochemical profiles and bioactivities of possible functional beverages produced from F. ulmaria and its alternative substitutes (F. camtschatica, F. denudata, F. stepposa). The investigated decoctions were analyzed regarding their macronutrient, carbohydrate, organic acid, amino acid and mineral composition...
December 26, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28034666/oxidative-stress-in-sepsis-pathophysiological-implications-justifying-antioxidant-co-therapy
#20
REVIEW
Carlos André Prauchner
Sepsis is one of the main causes of death among critically ill patients. Sepsis pathogenesis includes infection by gram-negative and gram-positive bacteria, fungi, or both; exacerbated inflammatory response; hypotension, with potential to cause vasodilatory shock; and lesser delivery of oxygen to tissues due to impairment of oxygen utilization by cells. The participation of reactive species and/or free radicals such as nitric oxide (NO), peroxynitrite (ONOO(-)), superoxide (O2(-)), hydrogen peroxide (H2O2), and hydroxyl radical (OH) has been reported to underlie these effects...
December 26, 2016: Burns: Journal of the International Society for Burn Injuries
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