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AdamTS 13

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https://www.readbyqxmd.com/read/28775254/adamts-13-regulates-neutrophil-recruitment-in-a-mouse-model-of-invasive-pulmonary-aspergillosis
#1
Astrid Alflen, Steve Prüfer, Katharina Ebner, Sebastian Reuter, Pamela Aranda Lopez, Inge Scharrer, Fumiaki Banno, Michael Stassen, Hansjörg Schild, Kerstin Jurk, Markus Bosmann, Hendrik Beckert, Markus P Radsak
Von Willebrand factor (VWF) is secreted as an acute phase protein during inflammation. ADAMTS-13 regulates the size and prothrombotic activity of VWF by it's specific proteolytic activity. To determine the relevance of this regulatory pathway for the innate inflammatory response by polymorphonuclear neutrophils (PMN), we employed a mouse model of invasive pulmonary aspergillosis (IPA) where PMN functionality is crucial for fungal clearance and survival. IPA was induced by intratracheal application of Aspergillus fumigatus (A...
August 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28765635/conditional-knockdown-of-hyaluronidase-2-in-articular-cartilage-stimulates-osteoarthritic-progression-in-a-mice-model
#2
Yoshitoshi Higuchi, Yoshihiro Nishida, Eiji Kozawa, Lisheng Zhuo, Eisuke Arai, Shunsuke Hamada, Daigo Morita, Kunihiro Ikuta, Koji Kimata, Takahiro Ushida, Naoki Ishiguro
The catabolism of hyaluronan in articular cartilage remains unclear. The aims of this study were to investigate the effects of hyaluronidase 2 (Hyal2) knockdown in articular cartilage on the development of osteoarthritis (OA) using genetic manipulated mice. Destabilization of the medial meniscus (DMM) model of Col2a promoter specific conditional Hyal2 knockout (Hyal (-/-) ) mice was established and examined. Age related and DMM induced alterations of articular cartilage of knee joint were evaluated with modified Mankin score and immunohistochemical staining of MMP-13, ADAMTS-5, KIAA11199, and biotinylated- hyaluronan binding protein staining in addition to histomorphometrical analyses...
August 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28761774/proinflammatory-and-anabolic-gene-expression-effects-of-platelet-rich-gel-supernatants-on-equine-synovial-membrane-explants-challenged-with-lipopolysaccharide
#3
Jorge U Carmona, Diana L Ríos, Catalina López, María E Álvarez, Jorge E Pérez
Platelet-rich plasma (PRP) preparations are used in horses with osteoarthritis (OA). However, some controversies remain regarding the ideal concentration of platelets and leukocytes to produce an adequate anti-inflammatory and anabolic response in the synovial membrane. The aims of this study were to study the influence of leukoconcentrated platelet-rich gel (Lc-PRG) and leukoreduced platelet-rich gel (Lr-PRG) supernatants on the quantitative expression of some proinflammatory and anabolic genes in equine synovial membrane explants (SMEs) challenged with lipopolysaccharide (LPS)...
2017: Veterinary Medicine International
https://www.readbyqxmd.com/read/28744817/knockdown-of-prmt1-suppresses-il-1%C3%AE-induced-cartilage-degradation-and-inflammatory-responses-in-human-chondrocytes-through-gli1-mediated-hedgehog-signaling-pathway
#4
Lei Xia, Hong-Xing Zhang, Mei-Li Xing, Yu-Ben Xu, Peng Li, Liang-Ku Huang, Jie Bai, Zhao Tian, Zan-Dong Zhao
Osteoarthritis (OA) is characterized by articular cartilage degradation and joint inflammation. The purpose of the present study is to elucidate the role of the specific function of PRMT1 in chondrocytes and its association with the pathophysiology of OA. We observed that the expression of PRMT1 was apparently upregulated in OA cartilage, as well as in chondrocytes stimulated with IL-1β. Additionally, knockdown of PRMT1 suppressed interleukin 1 beta (IL-1β)-induced extracellular matrix (ECM) metabolic imbalance by regulating the expression of MMP-13, ADAMTS-5, COL2A1, and ACAN...
July 25, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28743060/glucose-fluctuation-and-the-resultant-endothelial-injury-are-correlated-with-pancreatic-%C3%AE-cell-dysfunction-in-patients-with-coronary-artery-disease
#5
Makoto Murata, Hitoshi Adachi, Shigeru Oshima, Masahiko Kurabayashi
AIMS: We evaluated whether glucose fluctuation (GF) causes vascular endothelial injury and affects glucometabolic factors during lengthy oral glucose tolerance test (OGTT). METHODS: We enrolled consecutive 116 patients with coronary artery disease (CAD) who were performed coronary angiography and 4-h OGTT. Blood samples were collected before and 4h after glucose load to measure endothelial injury factor [von Willebrand factor (vWF) and vWF/a disintegrin-like and metalloproteinase with thrombospondin type-1 motifs 13 (ADAMTS-13) ratio]...
July 8, 2017: Diabetes Research and Clinical Practice
https://www.readbyqxmd.com/read/28724217/chondroprotective-and-anti-nociceptive-effects-of-caffeoylquinic-acid-in-osteoarthritis-by-downregulating-catabolic-activity-and-oxidative-damage-in-chondrocytes
#6
Luping Liu, Yongmei Li
We evaluated the chondroprotective and anti-nociceptive effects of caffeoylquinic acid (CA) in a rat osteoarthritis (OA) model. Monosodium iodoacetate was injected intra-articularly in the rats to produce OA. The rats were divided into three groups: control, OA group, which received vehicle, and the CA-treated group, which received CA (50mg/kg, p.o.) for 14days. At the end of the protocol, pain was estimated by observing the paw withdrawal threshold and latency. Degeneration of cartilage was assessed through histomorphological and microscopical examination of joints...
September 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28682993/quantification-of-von-willebrand-factor-cleavage-by-adamts-13-in-patients-supported-by-left-ventricular-assist-devices
#7
Yong Zhou, Shizhen Qin, Tristan Hilton, Li Tang, Miguel Cruz, Ruben Hernandez, Joel L Moake, Qiang Tian, O H Frazier, Jing-Fei Dong, Angelo Nascimbene
Patients supported by left ventricular assist devices (LVADs) often present with the loss of large von Willebrand factor (VWF) multimers. This VWF deficiency is believed to contribute to the bleeding diathesis of patients on LVAD support and is caused by excessive VWF cleavage by the metalloprotease ADAMTS-13 under high shear stress. However, only a small percentage of patients who have suffered the loss of large VWF multimers bleed. The actual rates of VWF cleavage in these patients have not been reported, primarily because of the lack of reliable detection methods...
July 1, 2017: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/28666239/cryptotanshinone-protects-against-il-1%C3%AE-induced-inflammation-in-human-osteoarthritis-chondrocytes-and-ameliorates-the-progression-of-osteoarthritis-in-mice
#8
Zhenhua Feng, Wenhao Zheng, Xiaobin Li, Jian Lin, Chenglong Xie, Hang Li, Liang Cheng, Aimin Wu, Wenfei Ni
Osteoarthritis (OA) is a common degenerative disease characterized by progressive erosion of articular cartilage, subchondral bone sclerosis and synovitis. Cryptotanshinone (CTS), an active component extracted from the root of Salvia miltiorrhiza Bunge, has been shown to have potent anti-inflammatory effects. However, its effects on OA have not been clearly elucidated. This study aimed to assess the effect of CTS on human OA chondrocytes and mice OA models. Human OA chondrocytes were pretreated with CTS (5, 10 and 20μM) for 2h and subsequently stimulated with IL-1β for 24h...
June 27, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28651806/von-willebrand-factor-deposition-and-adamts-13-consumption-in-allograft-tissue-of-thrombotic-microangiopathy-like-disorder-after-living-donor-liver-transplantation-a-case-report
#9
S Nakanuma, T Miyashita, H Hayashi, Y Ohbatake, H Takamura, M Okazaki, T Yamaguchi, S Sakai, I Makino, K Oyama, H Tajima, I Ninomiya, S Fushida, T Ohta
BACKGROUND: Thrombotic microangiopathy (TMA) pathogenesis after living donor liver transplantation (LDLT) is thought to be caused by release of unusually large von Willebrand factor multimers (UL-vWFMs) resulting from sinusoidal endothelial cell damage and induction of platelet adhesion and aggregation. A decrease in a disintegrin-like and metalloproteinase with thrombospondin type 1 motifs-13 (ADAMTS-13) that cleave UL-vWFMs might cause excessive UL-vWFMs activity and result in platelet thrombus formation...
June 23, 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28650450/endothelial-damage-is-aggravated-in-acute-gvhd-and-could-predict-its-development
#10
E Mir, M Palomo, M Rovira, A Pereira, G Escolar, O Penack, E Holler, E Carreras, M Diaz-Ricart
The aim of the present study was to explore whether there is enhanced endothelial dysfunction in patients developing acute GvHD (aGvHD) after allogeneic hematopoietic cell transplantation (allo-HCT) and to identify biomarkers with predictive and/or diagnostic value. In in vitro experiments, endothelial cells (ECs) were exposed to serum from patients with (aGvHD, n=31) and without (NoGvHD, n=13) aGvHD, to evaluate changes in surface adhesion receptors, the reactivity of the extracellular matrix by measuring the presence of Von Willebrand factor (VWF) and platelet adhesion, and the activation of intracellular signaling proteins...
June 26, 2017: Bone Marrow Transplantation
https://www.readbyqxmd.com/read/28647469/intra-articular-injection-of-microrna-140-mirna-140-alleviates-osteoarthritis-oa-progression-by-modulating-extracellular-matrix-ecm-homeostasis-in-rats
#11
H-B Si, Y Zeng, S-Y Liu, Z-K Zhou, Y-N Chen, J-Q Cheng, Y-R Lu, B Shen
OBJECTIVE: Disruptions of extracellular matrix (ECM) homeostasis are key events in the pathogenesis of osteoarthritis (OA). MicroRNA-140 (miRNA-140) is expressed specifically in cartilage and regulates ECM-degrading enzymes. Our objective in this study was to determine if intra-articular injection of miRNA-140 can attenuate OA progression in rats. DESIGN: miRNA-140 levels in human normal and OA cartilage derived chondrocytes and synovial fluid were assessed by polymerase chain reaction (PCR)...
June 21, 2017: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/28607779/thrombotic-microangiopathy-secondary-to-intravenous-abuse-of-opana%C3%A2-er
#12
Kamia Thakur, Vaibhav Agrawal, Ashley Kass, Lauren M Dimarino, R Patrick Dorion, Joseph Vadakara
Opana ER (oxymorphone) is an opioid drug available throughout the United States, and intravenous abuse of the crushed oral formulation has been associated with drug-induced thrombotic microangiopathy. In this abstract, we describe two young patients who lived together and used Opana ER intravenously. Both presented with microangiopathic hemolytic anemia that mimicked thrombotic thrombocytopenic purpura (TTP). Treating this condition poses a clinical challenge, as it is difficult to distinguish it from TTP. The role for plasma exchange is not clear but can be used while awaiting the results of the ADAMTS-13 activity, but ultimately supportive care with drug discontinuation is the recommended therapy of choice...
2017: Case Reports in Hematology
https://www.readbyqxmd.com/read/28585864/sustained-release-effects-of-berberine-loaded-chitosan-microspheres-on-in-vitro-chondrocyte-culture
#13
Yan Zhou, Shiqing Liu, Jianghua Ming, Yaming Li, Ming Deng, Bin He
The low bioavailability and short biological half-life of berberine chloride (BBR) negatively affect the protective role of this compound against osteoarthritis (OA). The present study was performed to evaluate the effectiveness of sustained BBR release system. Novel BBR-loaded chitosan microspheres (BBR-loaded CMs) were successfully synthesized using an ionic cross-linking method for sustained release. The basic characteristics of the prepared microspheres were subsequently evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) techniques, encapsulation efficiency (EE), and in vitro release experiments...
June 26, 2017: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/28570682/adamts-1-in-abdominal-aortic-aneurysm
#14
Emina Vorkapic, Maggie Folkesson, Kerstin Magnell, Mohammad Bohlooly-Y, Toste Länne, Dick Wågsäter
INTRODUCTION: Extracellular matrix degradation is a hallmark of abdominal aortic aneurysm (AAA). Among proteases that are capable of degrading extracellular matrix are a disintegrin and metalloproteases with thrombospondin motifs (ADAMTS). Pathogenesis of these proteases in AAA has not been investigated until date. METHODS AND RESULTS: Human aneurysmal and control aortas were collected and analyzed with RT-PCR measuring the ADAMTS-1, 4,5,6,8,9,10,13,17 and ADAMTSL-1...
2017: PloS One
https://www.readbyqxmd.com/read/28546076/sirna-knockdown-of-adamts-13-modulates-endothelial-cell-angiogenesis
#15
Huiyuan Tang, Manfai Lee, Eun Ho Kim, Daniel Bishop, George M Rodgers
ADAMTS-13, a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13, is a zinc-containing metalloprotease that cleaves von Willebrand factor (vWf). Previous publications by our laboratory have shown that ADAMTS-13 may also be involved in angiogenesis. For this study, we report the successful transient knockdown of endogenous ADAMTS-13 in human umbilical vein endothelial cells (HUVEC) via siRNA and the effects of reduced endogenous ADAMTS-13 on HUVEC angiogenesis functions. 15nM of ADAMTS-13 siRNA reduced HUVEC ADAMTS-13 protein levels by 90% after 24h incubation, whereas control siRNA did not affect endogenous ADAMTS-13 levels...
May 22, 2017: Microvascular Research
https://www.readbyqxmd.com/read/28545202/memantine-inhibits-degradation-of-the-articular-cartilage-extracellular-matrix-induced-by-advanced-glycation-end-products-ages
#16
Jijun Zhao, Yinghao Yu, Zhaofeng Wu, Ling Wang, Wei Li
The accumulation of inflammation and cartilage damage induced by advanced glycation end products (AGEs) are important hallmarks of osteoarthritis (OA). Memantine is a clinically licensed drug used for the treatment of moderate and severe Alzheimer's disease. To date, little information has been reported regarding the effects of memantine on cartilage destruction. In this study, we investigated the protective effects of memantine on AGE-induced degradation of collagen II and aggrecans in human SW1353 chondrocytes...
July 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28544596/exacerbation-of-aging-associated-and-instability-induced-murine-osteoarthritis-with-deletion-of-d-prostanoid-receptor-1-a-prostaglandin-d2-receptor
#17
Yassine Ouhaddi, Sarah-Salwa Nebbaki, Lauris Habouri, Hassan Afif, Bertrand Lussier, Mohit Kapoor, Shuh Narumiya, Jean-Pierre Pelletier, Johanne Martel-Pelletier, Mohamed Benderdour, Hassan Fahmi
OBJECTIVE: D prostanoid receptor 1 (DP1), a receptor for prostaglandin D2 , plays important roles in inflammation and cartilage metabolism. However, its role in the pathogenesis of osteoarthritis (OA) remains unknown. This study was undertaken to explore the roles of DP1 in the development of OA in murine models and to evaluate the efficacy of a DP1 selective agonist in the treatment of OA. METHODS: The development of aging-associated OA and destabilization of the medial meniscus (DMM)-induced OA was compared between DP1-deficient (DP1(-/-) ) and wild-type (WT) mice...
May 23, 2017: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/28508977/thrombotic-microangiopathy-caused-by-interferon-%C3%AE-1b-for-multiple-sclerosis-a-case-report
#18
Haruomi Nishio, Tatsuo Tsukamoto, Takeshi Matsubara, Yoichiro Okada, Ryosuke Takahashi, Motoko Yanagita
A 41-year-old man with a history of multiple sclerosis (MS) developed thrombotic microangiopathy after taking interferon β-1b for 10 years. Although the relapse of his MS was well controlled under normal blood pressure, he had persistent nausea, anorexia, gait disturbance and visual disorder 1 month before admission. He showed lethargy and high blood pressure (180/102 mmHg). Laboratory test results revealed anemia and thrombocytopenia, elevated LDH and renal dysfunction. Urinary dipstick showed a 2+ result for proteinuria and 3+ for hematuria...
November 2016: CEN Case Reports
https://www.readbyqxmd.com/read/28482435/-expression-of-nf-%C3%AE%C2%BAb-in-a-degenerative-human-intervertebral-disc-model
#19
X F Wang, A P Zhang, Z Y Sun, C Liu, L H Kuang, J W Tian
Objective: To investigate the changes in the expression of NF-κB signaling pathway in human degenerative intervertebral discs. Methods: From October 2014 to March 2016, 55 nucleus pulposus of surgical patients with degenerative human intervertebral disc were collected for study in Department of Orthopedic Surgey, Hospital of Zaozhuang Mining Corporation, and Department of Orthopedics, Shanghai General Hospital Affiliated Shanghai Jiaotong University, School of Medicine.The collected nucleus pulposus tissues were divided into two groups: experimental group(30) and control group(25)...
May 9, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28458159/celastrol-reduces-il-1%C3%AE-induced-matrix-catabolism-oxidative-stress-and-inflammation-in-human-nucleus-pulposus-cells-and-attenuates-rat-intervertebral-disc-degeneration-in-vivo
#20
Jian Chen, Jun Xuan, Yun-Tao Gu, Ke-Si Shi, Jun-Jun Xie, Jiao-Xiang Chen, Zeng-Ming Zheng, Yu Chen, Xi-Bang Chen, Yao-Sen Wu, Xiao-Lei Zhang, Xiang-Yang Wang
Celastrol has been reported to exert therapeutic potential on pro-inflammatory diseases including asthma, Crohn's disease, arthritis and neurodegenerative disorders via inhibiting NF-κB pathway. While the effect of celastrol on intervertebral disc degeneration (IDD), which is also a pro-inflammatory disease, remains unknown. In this study, we evaluated the effect of celastrol on IDD in IL-1β treated human nucleus pulposus cells in vitro as well as in puncture induced rat IDD model in vivo. Our results showed that celastrol reduced the expression of catabolic genes (MMP-3, 9, 13, ADAMTS-4, 5), oxidative stress factors (COX-2, iNOS) and pro-inflammatory factors (IL-6, TNF-a) induced by IL-1β in nucleus pulposus cells, also phosphorylation of IκBα and p65 were attenuated by celastrol, indicating NF-κB pathway was inhibited by celastrol in nucleus pulposus cells...
July 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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