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https://www.readbyqxmd.com/read/28930631/direct-evidence-of-intrinsic-blue-fluorescence-from-oligomeric-interfaces-of-human-serum-albumin
#1
Arpan Bhattacharya, Soumitra Bhowmik, Amit K Singh, Prashant Kodgire, Apurba K Das, Tushar Kanti Mukherjee
The molecular origin behind the concentration-dependent intrinsic blue fluorescence of human serum albumin (HSA) is not known yet. This unusual blue fluorescence is believed to be a characteristic feature of amyloid-like fibrils of protein/peptide and originates due to the delocalization of peptide bond electrons through the extended hydrogen bond networks of cross--sheet structure. Herein, by combining the results of spectroscopy, size exclusion chromatography, native gel electrophoresis and confocal microscopy, we have shown that the intrinsic blue fluorescence of HSA exclusively originates from oligomeric interfaces devoid of any amyloid-like fibrillar structure...
September 20, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28930474/unveiling-the-self-assembling-behavior-of-5-fluorouracil-and-its-n-n-dimethyl-derivative-a-spectroscopic-and-microscopic-approach
#2
Pavel Banerjee, Devdeep Mukherjee, Tapas Kumar Maiti, Nilmoni Sarkar
At physiological conditions, 5-fluorouracil (5-FU), an anti-cancer drug self-assembles into fibrils by strong hydrogen bonding network while its methylated derivative, 5-fluoro-1,3-dimethyluracil (5-FDMU) do not make fibril due to lack of strong hydrogen bonding motif. In vitro, 5-FU self-assembly is sensitive to physicochemical conditions like the pH and ionic strength of the solution, which tune the strength of the non-covalent driving forces. Here we report a surprising finding that the buffer-which is necessary to control the pH and is typically considered to be inert-also significantly, influences 5-FU self-assembly which indicates an important role of counter-ions in the fibril formation...
September 20, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28929203/effects-of-toothpastes-on-white-spot-lesions-around-orthodontic-brackets-using-quantitative-light-induced-fluorescence-qlf-an-in-vitro-study
#3
Gokcenur Gokce, Selcuk Savas, Ebru Kucukyilmaz, Ilknur Veli
AIM: The aim of this study was to investigate the effects of toothpastes with different contents on the remineralization of white spot lesions around orthodontic brackets by quantitative light-induced fluorescence (QLF). MATERIALS AND METHODS: A total of 45 freshly extracted human mandibular first molars were selected. Artificial caries lesions were created on enamel surfaces using an acidified gel system for 7 days. Initial relative fluorescence loss was calculated as the pretreatment (baseline) fluorescence value by QLF device...
September 19, 2017: Journal of Orofacial Orthopedics, Fortschritte der Kieferorthopädie
https://www.readbyqxmd.com/read/28928782/comparison-of-microleakage-of-scotchbond%C3%A2-universal-adhesive-with-methacrylate-resin-in-class-v-restorations-by-two-methods-swept-source-optical-coherence-tomography-and-dye-penetration
#4
Hamid Kermanshah, Hossein Khorsandian
BACKGROUND: One of the most important factors in restoration failure is microleakage at the restoration interface. Furthermore, a new generation of bonding, Scotchbond Universal (multi-mode adhesive), has been introduced to facilitate the bonding steps. The aim of this study was to compare the microleakage of Class V cavities restored using Scotchbond™ Universal with Scotchbond Multi-Purpose in two procedures. MATERIALS AND METHODS: Eighteen freshly extracted human molars were used in this study...
July 2017: Dental Research Journal
https://www.readbyqxmd.com/read/28926240/antineoplastic-agents-605-isoquinstatins
#5
George R Pettit, Noeleen Melody, Jean-Charles Chapuis
In order to further explore quinoline-type structural modification of the powerful anticancer drug dolastatin 10, an Indian Ocean sea hare constituent and parent molecule of the very successful antibody drug conjugate (ADC) Adcetris, our recent quinstatin study has been extended by replacing the quinoline ring with an isoquinoline. The resulting isoquinstatins (4-6) were modified to N-terminal desmethylisoquinstatins (7-9) and, in turn, bonded to appropriate linker units to give linker-desmethylisoquinstatin conjugates 11-13 in preparation for eventual monoclonal antibody attachment...
September 19, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28924948/magnetic-nanoparticles-for-the-delivery-of-dapagliflozin-to-hypoxic-tumors-physicochemical-characterization-and-cell-studies
#6
Athina Angelopoulou, Efstathia Voulgari, Argiris Kolokithas-Ntoukas, Aristides Bakandritsos, Konstantinos Avgoustakis
In solid tumors, hypoxia (lack of oxygen) is developed, which leads to the development of resistance of tumor cells to chemotherapy and radiotherapy through various mechanisms. Nevertheless, hypoxic cells are particularly vulnerable when glycolysis is inhibited. For this reason, in this study, the development of magnetically targetable nanocarriers of the sodium-glucose transporter protein (SGLT2) inhibitor dapagliflozin (DAPA) was developed for the selective delivery of DAPA in tumors. This nanomedicine in combination with radiotherapy or chemotherapy should be useful for effective treatment of hypoxic tumors...
September 18, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28924868/photodegradation-pathways-of-protein-disulfides-human-growth-hormone
#7
Daniel Steinmann, Olivier Mozziconacci, Rupesh Bommana, John F Stobaugh, Y John Wang, Christian Schöneich
PURPOSE: Comprehensive product characterization was performed for the photodegradation of protein disulfides, representatively of human growth hormone (somatotropin; hGH), in order to provide a product database, which will be useful for the general analysis of protein stability. METHODS: HGH was photo-irradiated at λ = 254 and λ > 295 nm and tryptic digests were analyzed by HPLC-MS to investigate light-induced disulfide degradation pathways. RESULTS: A total of 60 products were detected, and structures/tentative structures were assigned to the products by MS(2) and MS(3) analysis...
September 18, 2017: Pharmaceutical Research
https://www.readbyqxmd.com/read/28921442/preparation-of-immunoliposomes-by-direct-coupling-of-antibodies-based-on-a-thioether-bond
#8
Raquel Petrilli, Josimar O Eloy, Robert J Lee, Renata F V Lopez
Drug delivery is of paramount importance, since the drug needs to be delivered to a specific site, in adequate concentration, avoiding degradation in order to provide therapeutic efficacy. Different nanocarriers have been used over the years for this purpose and liposomes are well-established systems due to the high biocompatibility and the possibility to vehiculate both hydrophilic and lipophilic drugs. In order to circumvent the rapid clearance by the reticuloendothelial system and to avoid the healthy cells exposure to the drug, long circulating liposomes containing polyethyleneglycol (PEG) and functionalized liposomes for targeted delivery have been developed...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28920920/er-associated-degradation-is-required-for-vasopressin-prohormone-processing-and-systemic-water-homeostasis
#9
Guojun Shi, Diane Somlo, Geun Hyang Kim, Cristina Prescianotto-Baschong, Shengyi Sun, Nicole Beuret, Qiaoming Long, Jonas Rutishauser, Peter Arvan, Martin Spiess, Ling Qi
Peptide hormones are crucial regulators of many aspects of human physiology. Mutations that alter these signaling peptides are associated with physiological imbalances that underlie diseases. However, the conformational maturation of peptide hormone precursors (prohormones) in the ER remains largely unexplored. Here, we report that conformational maturation of proAVP, the precursor for the antidiuretic hormone arginine-vasopressin, within the ER requires the ER-associated degradation (ERAD) activity of the Sel1L-Hrd1 protein complex...
September 18, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28920684/stilbene-boronic-acids-form-a-covalent-bond-with-human-transthyretin-and-inhibit-its-aggregation
#10
Thomas P Smith, Ian W Windsor, Katrina T Forest, Ronald T Raines
Transthyretin (TTR) is a homotetrameric protein. Its dissociation into monomers leads to the formation of fibrils that underlie human amyloidogenic diseases. The binding of small molecules to the thyroxin-binding sites in TTR stabilizes the homotetramer and attenuates TTR amyloidosis. Herein, we report on boronic acid-substituted stilbenes that limit TTR amyloidosis in vitro. Assays of affinity for TTR and inhibition of its tendency to form fibrils were coupled with X-ray crystallographic analysis of nine TTR·ligand complexes...
September 18, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28920187/sub-ablative-er-cr-ysgg-laser-irradiation-under-all-ceramic-restorations-effects-on-demineralization-and-shear-bond-strength
#11
Serdar Bağlar
This study evaluated the caries resistant effects of sub-ablative Er,Cr:YSGG laser irradiation alone and combined with fluoride in comparison with fluoride application alone on enamel prepared for veneer restorations. And also, evaluated these treatments' effects on the shear bond strength of all-ceramic veneer restorations. One hundred and thirty-five human maxillary central teeth were assigned to groups of 1a-control, 1b-laser treated, 1c-fluoride treated, 1d-laser + fluoride treated for shear bond testing and to groups of 2a-positive control(non-demineralised), 2b-laser treated, 2c-fluoride treated, 2d-laser + fluoride treated, 2e-negative control (demineralised) for microhardness testing (n = 15, N = 135)...
September 17, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28919511/viscoelastic-properties-of-%C3%AE-keratin-fibers-in-hair
#12
Yang Yu, Wen Yang, Marc André Meyers
Considerable viscoelasticity and strain-rate sensitivity are a characteristic in α-keratin fibers, which can be considered a biopolymer. The understanding of viscoelasticity is an important part to the knowledge of the overall mechanical properties of these biological materials. Here, horse and human hairs are examined to analyze the sources of this response. The dynamic mechanical response of α-keratin fibers over a range of frequencies and temperatures is analyzed using a dynamic mechanical analyzer. The α-keratin fibers behave more elastically at higher frequencies while they become more viscous at higher temperatures...
September 14, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28919443/fv-clasp-an-artificially-designed-small-antibody-fragment-with-improved-production-compatibility-stability-and-crystallizability
#13
Takao Arimori, Yu Kitago, Masataka Umitsu, Yuki Fujii, Ryoko Asaki, Keiko Tamura-Kawakami, Junichi Takagi
Antibody fragments are frequently used as a "crystallization chaperone" to aid structural analysis of complex macromolecules that are otherwise crystallization resistant, but conventional fragment formats have not been designed for this particular application. By fusing an anti-parallel coiled-coil structure derived from the SARAH domain of human Mst1 kinase to the variable region of an antibody, we succeeded in creating a novel chimeric antibody fragment of ∼37 kDa, termed "Fv-clasp," which exhibits excellent crystallization compatibility while maintaining the binding ability of the original IgG molecule...
September 6, 2017: Structure
https://www.readbyqxmd.com/read/28918058/reducible-peg-pod-dna-nanoparticles-for-gene-transfer-in%C3%A2-vitro-and-in%C3%A2-vivo-application-in-a-mouse-model-of-age-related-macular-degeneration
#14
Bhanu Chandar Dasari, Siobhan M Cashman, Rajendra Kumar-Singh
Non-viral gene delivery systems are being developed to address limitations of viral gene delivery. Many of these non-viral systems are modeled on the properties of viruses including cell surface binding, endocytosis, endosomal escape, and nuclear targeting. Most non-viral gene transfer systems exhibit little correlation between in vitro and in vivo efficiency, hampering a systematic approach to their development. Previously, we have described a 3.5 kDa peptide (peptide for ocular delivery [POD]) that targets cell surface sialic acid...
September 15, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28917874/alginate-modified-with-maleimide-terminated-peg-as-drug-carriers-with-enhanced-mucoadhesion
#15
Yarden Shtenberg, Mor Goldfeder, Avi Schroeder, Havazelet Bianco-Peled
The goal of this study was to generate a new mucoadhesive carbohydrate-based delivery system composed of alginate (Alg) backbone covalently attached to polyethylene glycol (PEG) modified with a unique functional end-group (maleimide). The immobilization of PEG-maleimide chains significantly improved the mucoadhesion properties attributed to thioether bonds creation via Michael-type addition and hydrogen bonding with the mucus glycoproteins. Mucoadhesion studies using tensile and rotating cylinder assays revealed a 3...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917766/a-novel-colorimetric-and-ratiometric-fluorescent-probe-for-visualizing-so2-derivatives-in-environment-and-living-cells
#16
Zhuo Ye, Chong Duan, Ruilong Sheng, Junchao Xu, Hongying Wang, Lintao Zeng
Monitoring sulfur dioxide (SO2) derivatives in environment is of great significance due to their harmful effects to the environment and human health. In this study, a fluorescent probe (CZBT) for SO2 derivatives was prepared from 9-ethyl-9H-carbazole-3,6-dicarboxaldehyde and 2-methyl-benzothiazolium, which displayed a noticeable color change from yellow to colorless along with a remarkable fluorescence change from yellow to blue in response to HSO3(-). The probe could quantitatively determine the concentration of HSO3(-) with excellent selectivity, high sensitivity and low limit of detection...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28917116/thermal-and-photo-induced-degradation-of-perfluorinated-carboxylic-acids-kinetics-and-mechanism
#17
Jiaoqin Liu, Ruijuan Qu, Zunyao Wang, Itza Mendoza-Sanchez, Virender K Sharma
Perfluorinated carboxylic acids (PFCAs) of different carbon chain lengths are chemicals of concern to human health and their removal, using conventional remediation technologies, is challenging. The present paper pursuits thermal and photo-induced degradation of PFCAs (F(CF2)nCOOH, n = 1-9) under various concentrations of four different acids (HNO3, H2SO4, HCl, and H3PO4) covering a range of strong acidic to basic pH. For thermal-induced experiments, the temperature was set at 40 °C, 60 °C, and 80 °C at acid strengths of 0...
September 6, 2017: Water Research
https://www.readbyqxmd.com/read/28917051/selenocysteine-mediated-expressed-protein-ligation-of-selenom
#18
Jun Liu, Qingqing Chen, Sharon Rozovsky
A sizeable fraction of the selenoproteome encodes oxidoreductases possessing a thioredoxin fold, a structural motif that is shared among a diverse group of enzymes. In these oxidoreductases, the active site is comprised of a cysteine and a selenocysteine separated by one to two amino acids. In a subset of these selenoproteins, such as human SELENOH, SELENOM, SELENOT, SELENOV, SELENOW, and SELENOF, this redox motif is positioned immediately after the first β-sheet in a short loop, and is essential for interactions with its substrate or partners...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28916391/zika-virus-structural-biology-and-progress-in-vaccine-development
#19
REVIEW
Hsiao-Han Lin, Bak-Sau Yip, Li-Min Huang, Suh-Chin Wu
The growing number of zika virus (ZIKV) infections plus a 20-fold increase in neonatal microcephaly in newborns in Brazil have raised alarms in many countries regarding the threat to pregnant women. Instances of microcephaly and central nervous system malformations continue to increase in ZIKV outbreak regions. ZIKV is a small enveloped positive-strand RNA virus belonging to the Flavivirus genus of the Flaviviridae family. High-resolution ZIKV structures recently identified by cryo-electron microscopy indicate that the overall ZIKV structure is similar to those of other flaviviruses...
September 12, 2017: Biotechnology Advances
https://www.readbyqxmd.com/read/28915468/spectral-and-computational-features-of-the-binding-between-riparins-and-human-serum-albumin
#20
Cintia Ramos Camargo, Ícaro Putinhon Caruso, Stanley Juan Chavez Gutierrez, Marcelo Andres Fossey, José Maria Barbosa Filho, Marinônio Lopes Cornélio
The green Brazilian bay leaf, a spice much prized in local cuisine (Aniba riparia, Lauraceae), contains chemical compounds presenting benzoyl-derivatives named riparins, which have anti-inflammatory, antimicrobial and anxiolytic properties. However, it is unclear what kind of interaction riparins perform with any molecular target. As a profitable target, human serum albumin (HSA) is one of the principal extracellular proteins, with an exceptional capacity to interact with several molecules, and it also plays a crucial role in the transport, distribution, and metabolism of a wide variety of endogenous and exogenous ligands...
September 8, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
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