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Chengjie Xiong, Tao Li, Hui Kang, Hao Hu, Jing Han, Feng Xu
PURPOSE: Traditional open approach is an efficient way to treat lumbar spinal stenosis (SS) combined with disk herniation (DH); however, risk factors such as advanced age, osteoporosis etc. are associated with the complications after the surgery. This study aims to analyze the early clinical and radiological outcomes of treatment on SS&DH by using newly developed minimal invasive TESSYS-ISEE technique. METHODS: Patients with limp and unilateral lower limb radiculopathy underwent minimal invasive surgery by using TESSYS-ISEE technique...
June 16, 2018: European Spine Journal
Shuai Chen, Haifeng Zhu, Gangliang Wang, Ziang Xie, Jiying Wang, Jian Chen
PURPOSE: To evaluate the effects of leptin/leptin receptor (LepR) combined with mechanical stress on the development of ossification of the posterior longitudinal ligament (OPLL), which is a disease characterized by ectopic bone formation of the posterior longitudinal ligament (PLL) and can lead to radiculopathy and myelopathy. METHODS: Six human samples of the PLL were analyzed for the expression of leptin and LepR by RT-PCR and western blotting. PLL cells were stimulated with leptin and mechanical stress delivered via a Flexcell tension system, and osteogenic differentiation was evaluated by RT-PCR and western blotting analysis of osteogenic marker expression as well as by alkaline phosphatase (ALP) staining and alizarin red S staining...
June 16, 2018: European Spine Journal
Tomas Bakal, Jiri Janata, Lenka Sabova, Roman Grabic, Vladimir Zlabek, Lucie Najmanova
A robust and widely applicable method for sampling of aquatic microbial biofilm and further sample processing is presented. The method is based on next-generation sequencing of V4-V5 variable regions of 16S rRNA gene and further statistical analysis of sequencing data, which could be useful not only to investigate taxonomic composition of biofilm bacterial consortia but also to assess aquatic ecosystem health. Five artificial materials commonly used for biofilm growth (glass, stainless steel, aluminum, polypropylene, polyethylene) were tested to determine the one giving most robust and reproducible results...
June 16, 2018: Folia Microbiologica
Cheng-Ji Li, Fan Xia, Rong Wu, Hong-Sheng Tan, Hong-Xi Xu, Gang Xu, Hong-Bo Qin
Carnosic acid was used as starting material to synthesize royleanone derivatives featured C11-C14 para quinone. The importance of C-20 group of royleanone derivatives was verified by the cytotoxicity assay of royleanonic acid, miltionone I and deoxyneocrptotanshinone. Following our synthetic route, 15 amide derivatives were synthesized and 8 compounds exhibited moderate cytotoxic activities against three human cancer lines in vitro.
June 16, 2018: Natural Products and Bioprospecting
Mark Collins, Adam McGauvran, Bassem Elhassan
High-pressure injection injuries of the hand are rare, but potentially devastating injuries. The amount of tissue damage is dependent on the magnitude of the pressure generated by the device and the composition of the injected material. The standard of care is emergent surgical debridement of the injected material and devitalized tissue. Although, preoperative advanced imaging is not routinely pursued, MRI may be helpful in determining the anatomic distribution of the foreign material, and associated soft tissue or osseous injury...
June 16, 2018: Skeletal Radiology
Andrea Käppler, Marten Fischer, Barbara M Scholz-Böttcher, Sonja Oberbeckmann, Matthias Labrenz, Dieter Fischer, Klaus-Jochen Eichhorn, Brigitte Voit
In recent years, many studies on the analysis of microplastics (MP) in environmental samples have been published. These studies are hardly comparable due to different sampling, sample preparation, as well as identification and quantification techniques. Here, MP identification is one of the crucial pitfalls. Visual identification approaches using morphological criteria alone often lead to significant errors, being especially true for MP fibers. Reliable, chemical structure-based identification methods are indispensable...
June 16, 2018: Analytical and Bioanalytical Chemistry
Archana Shubhakar, Poh-Choo Pang, Daryl L Fernandes, Anne Dell, Daniel I R Spencer, Stuart M Haslam
Glycosylation is considered one of the most complex and structurally diverse post-translational modifications of proteins. Glycans play important roles in many biological processes such as protein folding, regulation of protein stability, solubility and serum half-life. One of the ways to study glycosylation is systematic structural characterizations of protein glycosylation utilizing glycomics methodology based around mass spectrometry (MS). The most prevalent bottleneck stages for glycomic analyses is laborious sample preparation steps...
June 16, 2018: Glycoconjugate Journal
Audrey Hammack, Rashed T Rihani, Bryan J Black, Joseph J Pancrazio, Bruce E Gnade
Substrate-integrated microelectrode arrays (MEAs) are non-invasive platforms for recording supra-threshold signals, i.e. action potentials or spikes, from a variety of cultured electrically active cells, and are useful for pharmacological and toxicological studies. However, the MEA substrate, which is often fabricated using semiconductor processing technology, presents some challenges to the user. Specifically, the electrode encapsulation, which may consist of a variety of inorganic and organic materials, requires a specific substrate preparation protocol to optimize cell adhesion to the surface...
June 16, 2018: Biomedical Microdevices
Xiaolin Liu, Yintao Xu, Lu Zhang, Ting Liu, Hui Zhang
BACKGROUND Ovarian cancer is the second most common malignant tumor of the female reproductive system and is the leading cause of death of gynecological malignancies, but at present there is no effective and safe therapy. There is no previously published report on the anti-cancer effect of prucalopride, which is a high-affinity 5-HT4 receptor. The aim of the present study was to determine whether prucalopride can inhibit proliferation of ovarian cancer cells. MATERIAL AND METHODS The cell viability was detected by use of the Cell Counting Kit-8 (CCK-8) assay...
June 17, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Yue Chen, Cheng Zhang, Jing Chen, Bohan Zhang, Hongqi Zhang, Xuetao Yang, Jingshu Liu, Qingchen Wu
BACKGROUND Transcription factor 21 (TCF21), a member of the class A of basic helix-loop-helix family, has been widely identified as a tumor suppressor. Growing evidence has demonstrated the downregulation of TCF21 in distinct cancers. The aim of this study was to explore the expression and biological functions of TCF21 in esophageal squamous cell carcinoma (ESCC). MATERIAL AND METHODS TCF21 expression in esophageal cancer cell lines and carcinomas tissues were detected, and its associations with clinical characteristics were analyzed...
June 17, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Ying Zhang, Qian Xin, Zhen Wu, Chaochao Wang, Yongbin Wang, Qian Wu, Rui Niu
BACKGROUND The present study was performed to explore the presence of informative protein biomarkers of human serum proteome in idiopathic pulmonary fibrosis (IPF). MATERIAL AND METHODS Serum samples were profiled using iTRAQ coupled with two-dimensional liquid chromatography/tandem mass spectrometry (2D-LC-MS/MS) technique, and ELISA was used to validate candidate biomarkers. RESULTS A total of 394 proteins were identified and 97 proteins were associated with IPF. Four biomarker candidates generated from iTRAQ experiments - CRP, fibrinogen-α chain, haptoglobin, and kininogen-1 - were successfully verified using ELISA...
June 17, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Leena Krogerus, Ivana Kholová
OBJECTIVE: The cell block (CB) technique refers to the processing of sediments, blood clots, or grossly visible tissue fragments from cytological specimens into paraffin blocks that can be cut and stained by the same methods used for histopathology. The technique brings additional tissue architectural information. CB can be used for ancillary techniques such as immunocytochemistry and molecular techniques. STUDY DESIGN: We reviewed the literature on the various preparatory techniques of CBs...
June 15, 2018: Acta Cytologica
Peng Peng, Shu-Zhuang Sun, Yu-Xiang Liu, Xin-Mei Liu, Svetlana Mintova, Zi-Feng Yan
The basis and contribution of mesopores created in ZSM-5 structures at different treatment conditions are systematically investigated. The results reveal that the mesopores originated from the alkali dissolution of pristine ZSM-5 are mainly intracrystalline and they contribute to excessive Brønsted acid sites, while the mesopores originated from the re-assembly of alkali dissolved aluminosilicate species possess Lewis acid sites. These ZSM-5 mesostructures showed an extended lifespan during the cracking of cumene (88...
June 9, 2018: Journal of Colloid and Interface Science
Mohamed A Farag, Mahmoud F Elsebai, Amira R Khattab
The growing interest in assuring phytomedicines efficacy and moreover the increase in requirements for its safety drive the development of analytical methods for its quality control assurance. Herein, we present a nuclear magnetic resonance (NMR) fingerprinting approach of artichoke leaf material from different origins and in its commercial preparations. Under optimized conditions, we were able to simultaneously identify 23 metabolites including sugars, amino and organic acids, sesquiterpene lactones, flavones, cinnamates, inulin, fatty acids and nitrogenous bases...
May 30, 2018: Journal of Pharmaceutical and Biomedical Analysis
Jian Zheng, Hong Zhang, Chun Wang
BACKGROUND: Intradiploic epidermoid cyst (IEC) concurrent with chronic epidural hematoma (CEDH) has never been reported in the literature. We report a unique case of IEC concurrent with anatomically close associated CEDH. CASE DESCRIPTION: A 54-year-old man presented with one week history of headache, confusion and drowsiness. Computed tomography (CT) exposed large extraaxial mixed density lesions with bone destruction and substantial mass effect in the left fronto-temporal region...
June 14, 2018: World Neurosurgery
Tyler J D'Ovidio, Rebecca M Roberts, Dixa Gautam, Zefram D Marks, Manju Saraswathy, Jeffrey W Stansbury, Devatha P Nair
The enhanced in situ photopolymerization kinetics of methyl methacrylate (MMA) to poly(methyl methacrylate) (PMMA) through the incorporation of both inert and reactive nanogel (NG) fillers under ambient conditions has been demonstrated. In addition to the polymerization kinetics, the physical and chemical properties of the prepolymeric NG were also utilized to tune the thermoplasticity and mechanical properties of the PMMA polymer network. The protocol followed in this study imparts superior MMA photopolymerization kinetics (≥ 60% double-bond conversion within 15 min for > 35 wt% nanogel loadings and ≥ 95% double-bond conversion in < 60 min for all NG concentrations) when compared with traditional polymerization mechanisms...
June 2, 2018: Journal of the Mechanical Behavior of Biomedical Materials
Maryam Odabaee, Mostafa Odabaee, Matthew Pelekanos, Gerhard Leinenga, Jürgen Götz
Ultrasound is increasingly being recognized as a neuromodulatory and therapeutic tool, inducing a broad range of bio-effects in the tissue of experimental animals and humans. To achieve these effects in a predictable manner in the human brain, the thick cancellous skull presents a problem, causing attenuation. In order to overcome this challenge, as a first step, the acoustic properties of a set of simple bone-modeling resin samples that displayed an increasing geometrical complexity (increasing step sizes) were analyzed...
June 1, 2018: Ultrasonics
Saikishore Meruva, Aditya B Singaraju, Bharath Kumar Gowdampally, Bhavani Prasad Vinjamuri, William C Stagner
Dipalmitoylphosphatidylcholine (DPPC) demonstrated complex differential scanning calorimetry (DSC) thermal behavior. Transitions below 100 °C showed variability in their thermotropic reversibility. An experimental design employing a DSC heat-cool-heat-cool-heat cycle and modulated DSC were used to gain insight into the DPPC's complex thermal nature. An annealing strategy was developed to reduce DPPC's thermotropic variability, moisture uptake rate, and rate variability. Samples annealed at 110 °C for 5 min provided a reproducible, thermally reversible material...
June 14, 2018: Journal of Pharmaceutical Sciences
J Wechsler
The « liquid biopsies » are samples of liquids such as blood, urine, spinal fluid that can contain tumor material. Clinical assays have been mainly focused on the peripheral blood containing circulating tumor cells and circulating tumor DNA. The circulating tumor cells are cancer cells released from the primary tumor or recurrences or metastases. They enter into the bloodstream after passing through the vessel wall. It is possible to analyze the circulating tumor cells by means of all cytologic and biomolecular techniques...
June 13, 2018: La Revue de Médecine Interne
Liset de la Fuente Rosales, Sebastien Incerti, Ziad Francis, Mario A Bernal
The use of Monte Carlo (MC) simulations remains a powerful tool to study the biological effects induced by ionizing radiation on living beings. Several MC codes are commonly used in research fields such as nanodosimetry, radiotherapy, radiation protection, and space radiation. This work presents an enhancement of an existing model [1] for radiobiological purposes, to account for the indirect DNA damage induced by ionizing particles. The Geant4-DNA simulation toolkit was used to simulate the physical, pre-chemical, and chemical stages of early DNA damage induced by protons and α-particles...
June 13, 2018: Physica Medica: PM
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