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https://www.readbyqxmd.com/read/28433658/molecular-cloning-of-esr2-and-gene-expression-analysis-of-esr1-and-esr2-in-the-pituitary-gland-of-the-chinese-alligator-alligator-sinensis-during-female-reproductive-cycle
#1
Ruidong Zhang, Yanan Yin, Long Sun, Peng Yan, Yongkang Zhou, Rong Wu, Xiaobing Wu
Estrogens play critical roles in reproductive physiology via estrogen receptors (ESRs) in vertebrates, including reptiles. Chinese alligator (Alligator sinensis) is an endemic and endangered reptile species in China. In the present study, we cloned ESR2 gene from the ovary using rapid amplification of cDNA ends (RACE), investigated the spatial expression of ESRs in various tissues and temporal expression of ESRs in the pituitary glands during the reproductive cycle in Chinese alligators by quantitative real-time PCR (qPCR)...
April 19, 2017: Gene
https://www.readbyqxmd.com/read/28429648/-the-influence-of-scene-context-on-parafoveal-processing-of-objects
#2
Monica S Castelhano, Effie J Pereira
Many studies in reading have shown the enhancing effect of context on the processing of a word before it is directly fixated (parafoveal processing of words; Balota et al., 1985; Balota & Rayner, 1983; Ehrlich & Rayner, 1981). Here, we examined whether scene context influences the parafoveal processing of objects and enhances the extraction of object information. Using a modified boundary paradigm (Rayner, 1975), the Dot-Boundary paradigm, participants fixated on a suddenly-onsetting cue before the preview object would onset 4° away...
April 21, 2017: Quarterly Journal of Experimental Psychology: QJEP
https://www.readbyqxmd.com/read/28429252/analysis-of-global-gene-expression-profiles-during-the-flowering-initiation-process-of-lilium%C3%A2-%C3%A3-%C3%A2-formolongi
#3
Yu-Fan Li, Ming-Fang Zhang, Meng Zhang, Gui-Xia Jia
The onset of flowering is critical for the reproductive development of plants. Lilium × formolongi is a lily hybrid that flowers within a year after sowing. We successfully identified four important stages during vegetative growth and flowering initiation of L. × formolongi under long day conditions. The plant tissues from the four stages were used in a genome-wide transcriptional analysis to investigate stage-specific changes of gene expression in L. × formolongi. In total, the sequence reads of the four RNA-sequencing libraries were assembled into 52,824 unigenes, of which 37,031 (70...
April 20, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28427933/high-molecular-weight-genomic-dna-mini-prep-for-filamentous-fungi
#4
Mi-Kyung Lee, Hee-Soo Park, Kap-Hoon Han, Seung-Beom Hong, Jae-Hyuk Yu
Purification of high quality genomic DNA (gDNA) from filamentous fungi suitable for whole genome sequencing has previously involved many steps. Here, we report a simple and easy-to-follow mini-preparation protocol for high molecular weight (∼20kb) gDNA from filamentous fungi including Aspergillus and Eurotium. This comprehensive protocol includes graphic step-by-step instructions for inoculation, homogenization, and purification of gDNA. The most critical step is a thorough 3-5min homogenization of the freeze-dried mycelium using a motorized hand-held homogenizer with a mini spatula inserted...
April 18, 2017: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/28427338/-working-is-out-of-the-question-a-qualitative-text-analysis-of-medical-certificates-of-disability
#5
Guri Aarseth, Bård Natvig, Eivind Engebretsen, Anne Kveim Lie
BACKGROUND: Medical certificates influence the distribution of economic benefits in welfare states; however, the qualitative aspects of these texts remain largely unexplored. The present study is the first systematic investigation done of these texts. Our aim was to investigate how GPs select and mediate information about their patients' health and how they support their conclusions about illness, functioning and fitness for work in medical certificates. METHODS: We performed a textual analysis of thirty-three medical certificates produced by general practitioners (GP) in Norway at the request of the Norwegian Labour and Welfare Administration (NAV)...
April 20, 2017: BMC Family Practice
https://www.readbyqxmd.com/read/28425742/comparing-and-validating-methods-of-reading-instruction-using-behavioural-and-neural-findings-in-an-artificial-orthography
#6
J S H Taylor, Matthew H Davis, Kathleen Rastle
There is strong scientific consensus that emphasizing print-to-sound relationships is critical when learning to read alphabetic languages. Nevertheless, reading instruction varies across English-speaking countries, from intensive phonic training to multicuing environments that teach sound- and meaning-based strategies. We sought to understand the behavioral and neural consequences of these differences in relative emphasis. We taught 24 English-speaking adults to read 2 sets of 24 novel words (e.g., /buv/, /sig/), written in 2 different unfamiliar orthographies...
April 20, 2017: Journal of Experimental Psychology. General
https://www.readbyqxmd.com/read/28425740/-healthier-than-thou-practicing-what-you-preach-backfires-by-increasing-anticipated-devaluation-correction-to-howe-and-monin-2017
#7
(no author information available yet)
Reports an error in "Healthier Than Thou? "Practicing What You Preach" Backfires by Increasing Anticipated Devaluation" by Lauren C. Howe and Benoît Monin (Journal of Personality and Social Psychology, Advanced Online Publication, Feb 27, 2017, np). In the article, the beginning phrase of the second paragraph of the Internal Meta-Analysis of Studies 3 Through 5 section is incorrect. It should instead begin as follows: Across the three studies. The Monin et al. (2014) reference in both the References list and in text is included in error...
May 2017: Journal of Personality and Social Psychology
https://www.readbyqxmd.com/read/28424086/questioning-the-application-of-risk-of-bias-tools-in-appraising-evidence-from-natural-experimental-studies-critical-reflections-on-benton-et-al-ijbnpa-2016
#8
David K Humphreys, Jenna Panter, David Ogilvie
We recently read the article by Benton et al. which reviewed risk of bias in natural experimental studies investigating the impact of the built environment on physical activity (Benton et al., 2016; Int J Behav Nutr Phys Act 13:107). As a technical exercise in assessing risk of bias to understand study quality, we found the results of this study both interesting and potentially useful. However, it prompted a number of concerns with the use of risk of bias tools for assessing the quality of evidence from studies exploiting natural experiments...
April 19, 2017: International Journal of Behavioral Nutrition and Physical Activity
https://www.readbyqxmd.com/read/28422593/fundamentals-in-biostatistics-for-investigation-in-pediatric-dentistry-part-ii-biostatistical-methods
#9
Amaury Pozos-Guillén, Socorro Ruiz-Rodríguez, Arturo Garrocho-Rangel
The main purpose of the second part of this series was to provide the reader with some basic aspects of the most common biostatistical methods employed in health sciences, in order to better understand the validity, significance and reliability of the results from any article on Pediatric Dentistry. Currently, as mentioned in the first paper, Pediatric Dentists need basic biostatistical knowledge to be able to apply it when critically appraise a dental article during the Evidence-based Dentistry (EBD) process, or when participating in the development of a clinical study with dental pediatric patients...
2017: Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/28421027/universal-visual-features-might-be-necessary-for-fluent-reading-a-longitudinal-study-of-visual-reading-in-braille-and-cyrillic-alphabets
#10
Łukasz Bola, Dominika Radziun, Katarzyna Siuda-Krzywicka, Joanna E Sowa, Małgorzata Paplińska, Ewa Sumera, Marcin Szwed
It has been hypothesized that efficient reading is possible because all reading scripts have been matched, through cultural evolution, to the natural capabilities of the visual cortex. This matching has resulted in all scripts being made of line-junctions, such as T, X, or L. Our aim was to test a critical prediction of this hypothesis: visual reading in an atypical script that is devoid of line-junctions (such as the Braille alphabet read visually) should be much less efficient than reading in a "normal" script (e...
2017: Frontiers in Psychology
https://www.readbyqxmd.com/read/28419659/making-visible-the-politics-and-ethics-of-alcohol-policy-research
#11
David Moore
Although research on alcohol policy has produced a huge international literature, alcohol research and policy itself-its cultural assumptions, methods, politics and ethics-has rarely been subject to critical analysis. In this article, I provide an appreciative review of an exception to this trend: Joseph Gusfield's 1981 classic, The Culture of Public Problems: Drinking-Driving and the Symbolic Order. I first outline Gusfield's argument that the 'problem of drinking-driving' is constructed as a 'drama of individualism' centring on the 'killer drunk'...
April 16, 2017: Addiction
https://www.readbyqxmd.com/read/28419628/hla-hd-an-accurate-hla-typing-algorithm-for-next-generation-sequencing-data
#12
Shuji Kawaguchi, Koichiro Higasa, Masakazu Shimizu, Ryo Yamada, Fumihiko Matsuda
The accurate typing of HLA alleles is critical for a variety of medical applications, such as genomic studies of multifactorial diseases, including immune system and inflammation-related disorders, and donor selection in organ transplantation and regenerative medicine. Here we developed a new algorithm for determining HLA alleles using next-generation sequencing (NGS) results. The method consists of constructing an extensive dictionary of HLA alleles, precise mapping of the NGS reads, and calculating a score based on weighted read counts to select the most suitable pair of alleles...
April 16, 2017: Human Mutation
https://www.readbyqxmd.com/read/28416672/bisulfite-converted-duplexes-for-the-strand-specific-detection-and-quantification-of-rare-mutations
#13
Austin K Mattox, Yuxuan Wang, Simeon Springer, Joshua D Cohen, Srinivasan Yegnasubramanian, William G Nelson, Kenneth W Kinzler, Bert Vogelstein, Nickolas Papadopoulos
The identification of mutations that are present at low frequencies in clinical samples is an essential component of precision medicine. The development of molecular barcoding for next-generation sequencing has greatly enhanced the sensitivity of detecting such mutations by massively parallel sequencing. However, further improvements in specificity would be useful for a variety of applications. We herein describe a technology (BiSeqS) that can increase the specificity of sequencing by at least two orders of magnitude over and above that achieved with molecular barcoding and can be applied to any massively parallel sequencing instrument...
April 17, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28416329/weight-stigma-predicts-inhibitory-control-and-food-selection-in-response-to-the-salience-of-weight-discrimination
#14
Ashley M Araiza, Joseph D Wellman
OBJECTIVE: Fear and stigmatization are often used to motivate individuals with higher body weight to engage in healthy behaviors, but these strategies are sometimes counterproductive, leading to undesirable outcomes. In the present study, the impact of weight-based stigma on cognition (i.e., inhibitory control) and food selection (i.e., calories selected) was examined among individuals who consider themselves to be overweight. It was predicted that participants higher in perceived weight stigma would perform more poorly on an inhibitory control task and order more calories on a food selection task when they read about discrimination against individuals with higher weight versus discrimination against an out-group...
April 14, 2017: Appetite
https://www.readbyqxmd.com/read/28416188/a-giraffe-neck-sign-of-the-medial-meniscus-a-characteristic-finding-of-the-medial-meniscus-posterior-root-tear-on-magnetic-resonance-imaging
#15
Takayuki Furumatsu, Masataka Fujii, Yuya Kodama, Toshifumi Ozaki
BACKGROUND: The posterior root ligament of the medial meniscus (MM) has a critical role in regulating the MM movement. An accurate diagnosis of the MM posterior root tear (MMPRT) using magnetic resonance imaging (MRI) is important for preventing sequential osteoarthritis following the MMPRT. However, diagnosis of the MMPRT is relatively difficult even after using several characteristic MRI findings. The aim of this study was to identify a useful meniscal body sign of the MMPRT for improving diagnostic MRI reading...
April 13, 2017: Journal of Orthopaedic Science: Official Journal of the Japanese Orthopaedic Association
https://www.readbyqxmd.com/read/28414628/fried-green-tomatoes-and-the-color-purple-a-case-study-in-lesbian-friendship-and-cultural-controversy
#16
Kristen Proehl
Published in the 1980s, Fannie Flagg's Fried Green Tomatoes at the Whistle Stop Café and Alice Walker's The Color Purple are lesbian coming-of-age narratives that share a great deal in common in terms of their thematic content, publication histories, and cultural afterlives. In both novels, female friendships are shaped by patriarchal violence and develop in the context of the rural, segregated, early twentieth-century U.S. South. But the two novels also diverge in significant ways, as differences in race, gender, class, and sexuality shape their protagonists' experiences of love and friendship...
April 17, 2017: Journal of Lesbian Studies
https://www.readbyqxmd.com/read/28411305/reading-charts-in-ophthalmology
#17
REVIEW
W Radner
A new generation of logarithmic reading charts has sparked interest in standardized reading performance analyses. Such reading charts have been developed according to the standards of the International Council of Ophthalmology. The print size progression in these calibrated charts is in accordance with the mathematical background of EN ISO 8596. These reading charts are: the Bailey-Lovie Word Reading Chart, the Colenbrander English Continuous Text Near Vision Cards, the Oculus Reading Probe II, the MNREAD Charts, the SKread Charts, and the RADNER Reading Charts...
April 14, 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/28410283/postoperative-evaluation-of-bilateral-reading-performance-with-two-intraocular-lenses-twelve-month-results
#18
Semra Akkaya Turhan, Ebru Toker
PURPOSE: The aim of this study was to compare the bilateral reading performances within the first 12 months after the implantation of two intraocular lens (IOL) models. METHODS: The patients involved in this research had bilateral phacoemulsification and implantation of the Acriva Reviol MFM 613 IOL (group 1) or AcrySof ReSTOR SN6AD1 apodized multifocal IOL (group 2). The bilateral reading performance was evaluated preoperatively and postoperatively using the MNREAD acuity charts...
April 12, 2017: Eye & Contact Lens
https://www.readbyqxmd.com/read/28402177/intersectional-harassment-and-deviant-embodiment-among-autistic-adults-dis-ability-gender-and-sexuality
#19
Jessica Penwell Barnett
Harassment scholarship increasingly attends to the intersectional nature of harassment and its function within systems of domination. However, little of this work includes disability. In-depth interviews with 24 adults on the autism spectrum in the USA demonstrate the intersections of gender, sexuality and (dis)ability in the construction of deviant embodiments as targets for harassment. These intersections also shape how participants made sense of these experiences of violence. Participants' disability characteristics were often read as gender or sexual variance, with harassers relying on sexist and heterosexist constructs to frighten, demean or humiliate them for disability characteristics...
April 12, 2017: Culture, Health & Sexuality
https://www.readbyqxmd.com/read/28401816/clinical-encounter-and-the-logic-of-relationality-reconfiguring-bodies-and-subjectivities-in-clinical-relations
#20
Karolina A Kazimierczak
This article critically examines the significance of relational approaches for sociological understandings of clinical interactions, relations and practices, by exploring the ways in which relational theories and concepts have been employed in the recent sociological accounts of clinical encounters to trouble the classical dyadic models of clinical interaction and the related atomistic conceptions of agency and accountability. Reading this work through the theoretical contributions from feminist science studies scholarship, and particularly the work of Donna Haraway and Karen Barad, the article proposes an alternative understanding of clinical interactions, relations and practices, where relations are conceived as constitutive of individuals (objects/bodies and their attributes/identities), rather than being constituted by encounters between individuals...
January 1, 2017: Health (London)
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