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https://www.readbyqxmd.com/read/29345200/distinct-niches-within-the-extracellular-matrix-dictate-fibroblast-function-in-cell-free-3d-lung-tissue-cultures
#1
Gerald Burgstaller, Arunima Sengupta, Sarah Vierkotten, Gerhard Preissler, Michael Lindner, Jürgen Behr, Melanie Königshoff, Oliver Eickelberg
Instructive cues from the extracellular matrix (ECM) and their functional interplay with cells play pivotal roles for development, tissue repair, and disease. However, the precise nature of this interplay remains elusive. We used an innovative 3D cell culture ECM model by decellularizing ex vivo lung tissue scaffolds (d3D-LTCs) derived from diseased and healthy mouse lungs, which widely mimics the native (patho)physiological in vivo microenvironment. We successfully repopulated all d3D-LTCs with primary human and murine fibroblasts...
January 18, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/29343714/endothelial-nitric-oxide-synthase-overexpressing-human-early-outgrowth-cells-inhibit-coronary-artery-smooth-muscle-cell-migration-through-paracrine-functions
#2
Sergio Guber, Talin Ebrahimian, Maryam Heidari, Nicoletta Eliopoulos, Stephanie Lehoux
Cells mobilized from the bone marrow can contribute to endothelial regeneration and repair. Nevertheless, cardiovascular diseases are associated with diminished numbers and function of these cells, attenuating their healing potential. Gene transfer of endothelial nitric oxide synthase (eNOS) can restore the activity of circulating cells. Furthermore, estrogen accelerates the reendothelialization capacity of early outgrowth cells (EOCs). We hypothesized that overexpressing eNOS alone or in combination with estrogen stimulation in EOCs would potentiate the beneficial effects of these cells in regulating smooth muscle cell (SMC) function...
January 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29325889/early-cell-response-of-osteogenic-cells-on-differently-modified-implant-surfaces-sequences-of-cell-proliferation-adherence-and-differentiation
#3
Sebastian Blatt, Andreas Max Pabst, Eik Schiegnitz, Marco Hosang, Thomas Ziebart, Christian Walter, Bilal Al-Nawas, Marcus Oliver Klein
OBJECTIVES: Osseointegration of dental implants is a crucial prerequisite for long-term survival. Therefore, surface modifications are needed to interact with the extracellular environment and to trigger osteogenic cell responses such as cell proliferation, adherence, and differentiation. The purpose of this study was to investigate different surface modifications in vitro over 2 weeks. MATERIALS AND METHODS: Commercially available cells from a human osteogenic cell line (HHOB-c) were cultivated on the following surfaces: titanium with smooth surfaces (polished titanium (P), machined titanium (M), polyetheretherketone (Peek)), titanium with rough and hydrophilised surfaces (acid etched titanium (A), sandblasted acid etched titanium (SA and SA2), sandblasted acid etched hydrophilised (SAH), titanium plasma painted titanium (TPS)), titanium with calcium phosphate-containing surfaces (titanium plasma painted calcium phosphate modified titanium (TPS-CaP), sandblasted calcium phosphate modified titanium (S-CaP), sandblasted acid etched calcium phosphate modified titanium (SA-CaP)), and zirconium-oxide (yttrium amplified zirconium (Z), yttrium amplified Ca2+ delivering zirconium (Z-Ca))...
December 26, 2017: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/29321258/functional-variants-in-the-lrrk2-gene-confer-shared-effects-on-risk-for-crohn-s-disease-and-parkinson-s-disease
#4
Ken Y Hui, Heriberto Fernandez-Hernandez, Jianzhong Hu, Adam Schaffner, Nathan Pankratz, Nai-Yun Hsu, Ling-Shiang Chuang, Shai Carmi, Nicole Villaverde, Xianting Li, Manual Rivas, Adam P Levine, Xiuliang Bao, Philippe R Labrias, Talin Haritunians, Darren Ruane, Kyle Gettler, Ernie Chen, Dalin Li, Elena R Schiff, Nikolas Pontikos, Nir Barzilai, Steven R Brant, Susan Bressman, Adam S Cheifetz, Lorraine N Clark, Mark J Daly, Robert J Desnick, Richard H Duerr, Seymour Katz, Todd Lencz, Richard H Myers, Harry Ostrer, Laurie Ozelius, Haydeh Payami, Yakov Peter, John D Rioux, Anthony W Segal, William K Scott, Mark S Silverberg, Jeffery M Vance, Iban Ubarretxena-Belandia, Tatiana Foroud, Gil Atzmon, Itsik Pe'er, Yiannis Ioannou, Dermot P B McGovern, Zhenyu Yue, Eric E Schadt, Judy H Cho, Inga Peter
Crohn's disease (CD), a form of inflammatory bowel disease, has a higher prevalence in Ashkenazi Jewish than in non-Jewish European populations. To define the role of nonsynonymous mutations, we performed exome sequencing of Ashkenazi Jewish patients with CD, followed by array-based genotyping and association analysis in 2066 CD cases and 3633 healthy controls. We detected association signals in the LRRK2 gene that conferred risk for CD (N2081D variant, P = 9.5 × 10-10) or protection from CD (N551K variant, tagging R1398H-associated haplotype, P = 3...
January 10, 2018: Science Translational Medicine
https://www.readbyqxmd.com/read/29316790/unraveling-the-semiconducting-metallic-discrepancy-in-ni3-hitp-2
#5
Michael E Foster, Karl Sohlberg, Mark D Allendorf, A Alec Talin
Ni3(2,3,6,7,10,11-hexaiminotriphenylene)2 is a π-stacked layered metal-organic framework material with extended π-conjugation that is analogous to graphene. Published experimental results indicate that the material is semiconducting, but all theoretical studies to-date predict the bulk material to be metallic. Given that previous experimental work was carried out on specimens containing complex nanocrystalline microstructures, and the tendency for internal interfaces to introduce transport barriers, we apply DFT to investigate the influence of internal interface defects on the electronic structure of bulk Ni3(HITP)2...
January 10, 2018: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/29285101/proteomic-changes-of-cd4-cd25-forkhead-box-p3-regulatory-t-cells-in-a-30-day-rat-model-of-sepsis-survival
#6
Yuxia Jiao, Siqi Tan, Junyu Xiong
Sepsis is defined as life threatening organ dysfunction arising from a dysregulated host response to infection. The outcomes of sepsis include early mortality, delayed mortality and recovery, and depend on the inflammatory response. Previous studies have demonstrated that regulatory T cells (Tregs) are important in determining the outcome of sepsis, as their suppressive function serves a role in maintaining immune homeostasis. However, Treg-mediated immunosuppression during the course of sepsis remains unclear and little is known about the survival of patients following diagnosis...
December 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29276177/integrin-bound-talin-head-inhibits-actin-filament-barbed-end-elongation
#7
Corina Ciobanasu, Hong Wang, Véronique Henriot, Cécile Mathieu, Annabelle Fente, Sandrine Csillag, Clémence Vigouroux, Bruno Faivre, Christophe Le Clainche
Focal adhesions (FAs) mechanically couple the extracellular matrix (ECM) to the dynamic actin cytoskeleton, via transmembrane integrins and actin-binding proteins. The molecular mechanisms by which protein machineries control force transmission along this molecular axis, i.e. modulating integrin activation and controlling actin polymerization, remain largely unknown. Talin is a major actin-binding protein that controls both the inside-out activation of integrins and actin-filament anchoring and thus plays a major role in the establishment of the actin-ECM mechanical coupling...
December 24, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29274201/itraq-based-proteomic-analysis-reveals-protein-profile-in-plasma-from-children-with-autism
#8
Liming Shen, Kaoyuan Zhang, Chengyun Feng, Youjiao Chen, Shuiming Li, Javed Iqbal, Liping Liao, Yuxi Zhao, Jian Zhai
PURPOSE: Autism is a childhood neurological disorder with poorly understood etiology and pathology. This study is designed to identify differentially expressed proteins which might serve as potential biomarkers for autism. EXPERIMENTAL DESIGN: We performed iTRAQ (isobaric tags for relative and absolute quantitation) analysis for normal and autistic children's plasma of the same age group. RESULTS: The results showed that 24 differentially expressed proteins were identified between autistic subjects and controls...
December 23, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/29261089/the-collaborative-ocular-tuberculosis-study-cots-1-report-3-polymerase-chain-reaction-in-the-diagnosis-and-management-of-tubercular-uveitis-global-trends
#9
Aniruddha Agarwal, Rupesh Agrawal, Dinesh Visva Gunasekaran, Dhananjay Raje, Bhaskar Gupta, Kanika Aggarwal, Somasheila L Murthy, Mark Westcott, Soon Phaik Chee, Peter Mccluskey, Ho Su Ling, Stephen Teoh, Luca Cimino, Jyotirmay Biswas, Shishir Narain, Manisha Agarwal, Padmamalini Mahendradas, Moncef Khairallah, Nicholas Jones, Ilknur Tugal-Tutkun, Kalpana Babu, Soumayava Basu, Ester Carreño, Richard Lee, Hassan Al-Dhibi, Bahram Bodaghi, Alessandro Invernizzi, Debra A Goldstein, Carl P Herbort, Talin Barisani-Asenbauer, Julio J González-López, Sofia Androudi, Reema Bansal, Bruttendu Moharana, Sarakshi Mahajan, Simona Esposti, Anastasia Tasiopoulou, Sengal Nadarajah, Mamta Agarwal, Sharanya Abraham, Ruchi Vala, Ramandeep Singh, Aman Sharma, Kusum Sharma, Manfred Zierhut, Onn Min Kon, Emmett Cunningham, Quan Dong Nguyen, Carlos Pavesio, Vishali Gupta
PURPOSE: To analyze the role of polymerase chain reaction (PCR) of ocular fluids in management of tubercular (TB) anterior, intermediate, posterior, and panuveitis. METHODS: In Collaborative Ocular Tuberculosis Study (COTS)-1 (25 centers, n = 962), patients with TB-related uveitis were included. 59 patients undergoing PCR of intraocular fluids (18 females; 53 Asian Indians) were included. RESULTS: 59 (6.13%) of COTS-1 underwent PCR analysis...
December 20, 2017: Ocular Immunology and Inflammation
https://www.readbyqxmd.com/read/29259127/activated-heterotrimeric-g-protein-%C3%AE-i-subunits-inhibit-rap-dependent-cell-adhesion-and-promote-cell-migration
#10
Jesi Y To, Alan V Smrcka
Our recent work uncovered novel roles for activated Gαi signaling in regulation of neutrophil polarity and adhesion. GαiGTP-dependent enhancement neutrophil polarization was dependent on inhibition of cAMP/PKA signaling, while reversal of Gβγ stimulated adhesion was cAMP/PKA-independent. To uncover the mechanism for Gαi regulation of adhesion, we analyzed the effects of constitutively active Gαi1(Q204L) expression on adhesion driven by constitutively active Rap1a(G12V) or its downstream effector Radil in neutrophil-like HL60 cells, or in HT-1080 fibrosarcoma cells...
December 19, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29251413/serologic-genetic-and-clinical-associations-with-increased-healthcare-resource-utilization-in-inflammatory-bowel-disease
#11
Phillip Gu, Anshika Kapur, Dalin Li, Talin Haritunians, Eric Vasiliauskas, David Q Shih, Stephan R Targan, Brennan M R Spiegel, Dermot P B McGovern, Jeanne T Black, Gil Y Melmed
BACKGROUND: Inflammatory bowel diseases (IBD) are associated with significant morbidity and economic burden. The highly variable course of IBD creates a need for accurate predictors of clinical outcomes and health resource utilization (HRU) to guide treatment decisions early in the disease course. OBJECTIVE: We aimed to identify clinical, serologic and/or genetic markers associated with inpatient resource utilization in patients with ulcerative colitis (UC) and Crohn's disease (CD)...
December 18, 2017: Journal of Digestive Diseases
https://www.readbyqxmd.com/read/29212803/elastase-alters-contractility-and-promotes-an-inflammatory-synthetic-phenotype-in-airway-smooth-muscle-tissues
#12
Angelia D Lockett, Yidi Wu, Susan J Gunst
Neutrophil elastase is secreted by inflammatory cells during airway inflammation and can elicit airway hyperactivity in vivo. Elastase can degrade multiple components of the extracellular matrix. We hypothesized that elastase might disrupt the connections between airway smooth muscle (ASM) cells and the extracellular matrix, and that this might have direct effects on ASM tissue responsiveness and inflammation. The effect of elastase treatment on ASM contractility was assessed in vitro in isolated strips of canine tracheal smooth muscle by stimulating tissues with cumulatively increasing concentrations of ACh and measuring contractile force...
December 6, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/29202952/factors-influencing-pediatric-outpatient-transthoracic-echocardiography-utilization-before%C3%A2-appropriate-use-criteria-release-a%C3%A2-multicenter-study
#13
Kenan W D Stern, Talin Gulesserian, Jaeun Choi, Sean M Lang, Christopher J Statile, Erik C Michelfelder, Ericka S McLaughlin, Tuan Nguyen, Leo Lopez, George R Verghese, Daphne T Hsu, Ritu Sachdeva
BACKGROUND: Although pediatric appropriate use criteria (AUC) for outpatient transthoracic echocardiography (TTE) are available, little is known about TTE utilization patterns before their release. The aims of this study were to determine the relation between AUC and TTE utilization and to identify patient and physician factors associated with discordance between the AUC and clinical practice. METHODS: A retrospective review of 3,000 initial outpatient pediatric cardiology encounters at six centers was performed...
December 2017: Journal of the American Society of Echocardiography
https://www.readbyqxmd.com/read/29201280/a-network-approach-to-examining-injury-severity-in-pediatric-tbi
#14
Emily L Dennis, Faisal Rashid, Neda Jahanshad, Talin Babikian, Richard Mink, Christopher Babbitt, Jeffrey Johnson, Christopher C Giza, Robert F Asarnow, Paul M Thompson
Traumatic brain injury (TBI) is the leading cause of death and disability in children, and can lead to long lasting functional impairment. Many factors influence outcome, but imaging studies examining effects of individual variables are limited by sample size. Roughly 20-40% of hospitalized TBI patients experience seizures, but not all of these patients go on to develop a recurrent seizure disorder. Here we examined differences in structural network connectivity in pediatric patients who had sustained a moderate-severe TBI (msTBI)...
2017: Proceedings of the IEEE International Symposium on Biomedical Imaging: from Nano to Macro
https://www.readbyqxmd.com/read/29194184/biomechanical-comparison-of-loaded-countermovement-jumps-performed-on-land-and-in-water
#15
Talin Louder, Eadric Bressel, Clint Nardoni, Dennis Dolny
Researchers have observed physical improvements following the completion of aquatic-based jump training. However, there is a lack of research on the biomechanical specificity of aquatic-based movement. Therefore, the purpose of this investigation was to evaluate the kinetics and kinematics of loaded countermovement jumps performed in water versus land. Twenty young males and twenty-four NCAA division I women's soccer and gymnastics athletes were asked to perform unloaded and loaded countermovement jumps on land and in chest-deep water immersion...
November 29, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/29177310/non-gaussian-elasticity-and-charge-density-dependent-swelling-of-strong-polyelectrolyte-poly-n-isopropylacrylamide-co-sodium-acrylate-hydrogels
#16
Nermin Orakdogen, Talin Boyacı
The mechanical properties and charge density-dependent swelling of strong polyelectrolyte poly(N-isopropylacrylamide-co-sodium acrylate) P(NIPA-co-NaA) hydrogels prepared at a fixed total monomer concentration and crosslinker ratio, but at various charge densities, i.e. NaA content in the feed between 0 and 90 mol%, were investigated. The elasticity results were discussed to explain the relationship between the elastic free energy ΔGel and the swelling ratio α as well as to fit the existing theories to the swelling data...
November 27, 2017: Soft Matter
https://www.readbyqxmd.com/read/29176673/phosphoproteomics-of-camp-signaling-of-bordetella-adenylate-cyclase-toxin-in-mouse-dendritic-cells
#17
Jakub Novák, Ivo Fabrik, Irena Linhartová, Marek Link, Ondřej Černý, Jiří Stulík, Peter Šebo
The adenylate cyclase toxin (CyaA) of the whooping cough agent Bordetella pertussis subverts immune functions of host myeloid cells expressing the αMβ2 integrin (CD11b/CD18, CR3 or Mac-1). CyaA delivers into cytosol of cells an extremely catalytically active adenylyl cyclase enzyme, which disrupts the innate and adaptive immune functions of phagocytes through unregulated production of the key signaling molecule cAMP. We have used phosphoproteomics to analyze cAMP signaling of CyaA in murine bone marrow-derived dendritic cells...
November 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29170462/structure-of-rap1b-bound-to-talin-reveals-a-pathway-for-triggering-integrin-activation
#18
Liang Zhu, Jun Yang, Thomas Bromberger, Ashley Holly, Fan Lu, Huan Liu, Kevin Sun, Sarah Klapproth, Jamila Hirbawi, Tatiana V Byzova, Edward F Plow, Markus Moser, Jun Qin
Activation of transmembrane receptor integrin by talin is essential for inducing cell adhesion. However, the pathway that recruits talin to the membrane, which critically controls talin's action, remains elusive. Membrane-anchored mammalian small GTPase Rap1 is known to bind talin-F0 domain but the binding was shown to be weak and thus hardly studied. Here we show structurally that talin-F0 binds to human Rap1b like canonical Rap1 effectors despite little sequence homology, and disruption of the binding strongly impairs integrin activation, cell adhesion, and cell spreading...
November 23, 2017: Nature Communications
https://www.readbyqxmd.com/read/29153504/pressure-dependent-chemical-shifts-in-the-r3-domain-of-talin-show-that-it-is-thermodynamically-poised-for-binding-to-either-vinculin-or-riam
#19
Nicola J Baxter, Thomas Zacharchenko, Igor L Barsukov, Mike P Williamson
Talin mediates attachment of the cell to the extracellular matrix. It is targeted by the Rap1 effector RIAM to focal adhesion sites and subsequently undergoes force-induced conformational opening to recruit the actin-interacting protein vinculin. The conformational switch involves the talin R3 domain, which binds RIAM when closed and vinculin when open. Here, we apply pressure to R3 and measure (1)H, (15)N, and (13)C chemical shift changes, which are fitted using a simple model, and indicate that R3 is only 50% closed: the closed form is a four-helix bundle, while in the open state helix 1 is twisted out...
November 10, 2017: Structure
https://www.readbyqxmd.com/read/29149383/diffusion-mri-in-pediatric-brain-injury
#20
Emily L Dennis, Talin Babikian, Christopher C Giza, Paul M Thompson, Robert F Asarnow
Traumatic brain injury (TBI) is a major public health issue around the world and can be especially devastating in children as TBI can derail cognitive and social development. White matter (WM) is particularly vulnerable to disruption post-TBI, as myelination is ongoing during this period. Diffusion magnetic resonance imaging (dMRI) is a versatile modality for identifying and quantifying WM disruption and can detect diffuse axonal injury (DAI or TAI (traumatic axonal injury)). This review covers dMRI studies of pediatric TBI, including mild to severe injuries, and covering all periods post-injury...
October 2017: Child's Nervous System: ChNS: Official Journal of the International Society for Pediatric Neurosurgery
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