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MTA study

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https://www.readbyqxmd.com/read/28528141/portland-cement-induces-human-periodontal-ligament-cells-to-differentiate-by-upregulating-mir-146a
#1
Min-Ching Wang, Li-Yin Yeh, Wen-Yu Shih, Wan-Chun Li, Kuo-Wei Chang, Shu-Chun Lin
BACKGROUND/PURPOSE: Bioaggregates such as Portland cement (PC) can be an economical alternative for mineral trioxide aggregate (MTA) with additional benefit of less discoloration. MTA has been known to induce differentiations of several dental cells. MicroRNAs are important regulators of biological processes, including differentiation, physiologic homeostasis, and disease progression. This study is to explore how PC enhances the differentiation of periodontal ligament (PDL) cells in microRNAs level...
May 18, 2017: Journal of the Formosan Medical Association, Taiwan Yi Zhi
https://www.readbyqxmd.com/read/28525679/retrospective-evaluation-of-healing-of-periapical-lesions-after-unintentional-extrusion-of-mineral-trioxide-aggregate
#2
Levent Demiriz, Ebru Hazar Bodrumlu
BACKGROUND: During the apexification procedure for teeth with open apices, mineral trioxide aggregate (MTA) may be unintentionally extruded. The aim of the present study was the retrospective evaluation of the healing of periapical lesions in permanent incisor teeth with open apices after the unintentional extrusion of MTA. METHODS: The clinical and radiographic records of 55 maxillary permanent central teeth treated by MTA apexification were evaluated. Filled teeth with unintentionally extruded MTA were selected as group 1 (n = 21), whereas the teeth with no MTA extrusion were selected as group 2 (n = 34)...
May 19, 2017: Journal of Applied Biomaterials & Functional Materials
https://www.readbyqxmd.com/read/28523639/association-mapping-of-yield-and-yield-related-traits-under-reproductive-stage-drought-stress-in-rice-oryza-sativa-l
#3
B P Mallikarjuna Swamy, Noraziyah Abd Aziz Shamsudin, Site Noorzuraini Abd Rahman, Ramil Mauleon, Wickneswari Ratnam, Ma Teressa Sta Cruz, Arvind Kumar
BACKGROUND: The identification and introgression of major-effect QTLs for grain yield under drought are some of the best and well-proven approaches for improving the drought tolerance of rice varieties. In the present study, we characterized Malaysian rice germplasm for yield and yield-related traits and identified significant trait marker associations by structured association mapping. RESULTS: The drought screening was successful in screening germplasm with a yield reduction of up to 60% and heritability for grain yield under drought was up to 78%...
December 2017: Rice
https://www.readbyqxmd.com/read/28518212/evaluation-of-cytotoxicity-and-ph-changes-generated-by-various-dental-pulp-capping-materials-an-in-vitro-study
#4
Elzbieta Luczaj-Cepowicz, Grazyna Marczuk-Kolada, Malgorzata Pawinska, Marta Obidzinska, Adam Holownia
INTRODUCTION: Various materials are used in direct dental pulp capping method. Their biocompatibility and alkalizing abilities are of primary importance affecting therapeutic effects. The aim of this study was to evaluate and compare the cytotoxicity of various pulp-capping materials on human gingival fibroblasts and investigate the pH changes induced by these materials. MATERIAL AND METHODS: Human gingival fibroblasts were cultured with nine direct pulp materials using culture plate inserts...
May 18, 2017: Folia Histochemica et Cytobiologica
https://www.readbyqxmd.com/read/28513520/clinical-assessment-of-mineral-trioxide-aggregate-in-the-treatment-of-deep-carious-lesions
#5
U K Vural, A Kiremitçi, S Gökalp
OBJECTIVE: This clinical study aimed to assess the effectiveness of mineral trioxide aggregate (MTA) and calcium hydroxide (Ca[OH]2) in the treatment of deep carious lesions using the direct complete caries excavation technique. SUBJECTS AND METHODS: Hundred permanent molars/premolars were capped either with Ca (OH)2 (n = 49) or MTA (n = 51) and restored with composite resin in 73 patients. Periapical radiographs were taken prior to the treatment, at 6 months and 12 months...
May 2017: Nigerian Journal of Clinical Practice
https://www.readbyqxmd.com/read/28513477/microtube-array-membrane-bioreactor-promotes-neuronal-differentiation-and-orientation
#6
Sabrina Morelli, Antonella Piscioneri, Simona Salerno, Chien-Chung Chen, Chee Ho Chew, Lidietta Giorno, Enrico Drioli, Loredana De Bartolo
An important challenge in neuronal tissue engineering is to create innovative tools capable of promoting cellular response in terms of neuronal differentiation and neurite orientation that may be used as investigational platforms for studying neurobiological events and neurodegenerative disorders. A novel membrane bioreactor was created to provide a 3D well-controlled microenvironment for neuronal outgrowth. The bioreactor consisted of poly-L-lactic acid highly aligned microtube array (PLLA-MTA) membranes assembled in parallel within a chamber that establish an intraluminal and an extraluminal compartment whose communication occurs through the pores of the MTA membrane walls...
May 17, 2017: Biofabrication
https://www.readbyqxmd.com/read/28512536/effect-of-smear-clear-and-some-other-commonly-used-irrigants-on-dislodgement-resistance-of-mineral-trioxide-aggregate-to-root-dentin
#7
Mona Sadegh, Hasti Sohrabi, Mohamadjavad Kharazifard, Farzaneh Afkhami
BACKGROUND: This study aimed to assess the push-out bond strength of mineral trioxide aggregate (MTA) to root canal dentin after irrigation with Smear Clear in comparison with 2.5% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX) and saline as commonly used root canal irrigants. MATERIAL AND METHODS: The coronal and mid-root areas of maxillary anterior teeth were horizontally sectioned into one-millimeter thick slices. The root canal lumen of dentinal slices was dilated using a diamond bur with 1...
May 2017: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/28512495/endodontic-management-of-open-apex-teeth-using-lyophilized-collagen-sponge-and-mta-cement-report-of-two-cases
#8
Miriam Graziele Magro, Milton Carlos Kuga, Weber Adad Ricci, Kátia Cristina Keine, Mateus Rodrigues Tonetto, Suellen Linares Lima, Alvaro Henrique Borges, Lauriê Garcia Belizário, Matheus Coêlho Bandeca
Teeth with open apices, such as in immature teeth or those with apical root resorption are clinical cases with difficult immediate resolution. With the use of mineral trioxide aggregate (MTA) in dentistry, it was possible to optimize the treatment time of these cases by immediate placement of apical plug and the root canal filling. However, some negative effects can occur if MTA is extruded beyond the apex. To avoid this accident, it has been recommended to use of an apical matrix prior to placement of MTA...
2017: Iranian Endodontic Journal
https://www.readbyqxmd.com/read/28512492/in-vitro-sealing-properties-of-calcium-enriched-mixture-and-mineral-trioxide-aggregate-orifice-barriers-during-intra-coronal-bleaching
#9
Negar Moghadam, Amir Ardalan Abdollahi, Hoda Aghabalayi Fakhim, Zahra Borna
INTRODUCTION: This study aimed at evaluating the sealing properties of calcium-enriched mixture (CEM) compared to mineral trioxide aggregate (MTA) as a cervical barriers in intra-coronal bleaching. METHODS AND MATERIALS: In this in vitro study, endodontic treatment was performed on 60 extracted human incisors and canines without canal calcification, caries, restorations, resorption or cracks. The teeth were then randomly divided into two experimental groups and two control groups (n=15)...
2017: Iranian Endodontic Journal
https://www.readbyqxmd.com/read/28512491/pulp-response-following-direct-pulp-capping-with-dentin-adhesives-and-mineral-trioxide-aggregate-an-animal-study
#10
Ali Akhavan, Farahnaz Arbabzadeh, Majid Bouzari, Sayed Mohammad Razavi, Amin Davoudi
INTRODUCTION: Pulp vitality and its continuous dentin prodution are essential for long-term success of direct pulp capping (DPC). The aim of present study was to evaluate the histopathological response of the canine pulp following DPC using either different dentin adhesive resins (DAR), calcium hydroxide (CH) or mineral trioxide aggregate (MTA). METHODS AND MATERIALS: DPC was done on 72 dog's teeth using 6 types of dental materials (n=12) (4 types of DAR, white MTA and CH)...
2017: Iranian Endodontic Journal
https://www.readbyqxmd.com/read/28512488/in-vitro-microleakage-of-mineral-trioxide-aggregate-calcium-enriched-mixture-cement-and-biodentine-intra-orifice-barriers
#11
Fatemeh Ramezanali, Sasan Aryanezhad, Fatemeh Mohammadian, Fatemeh Dibaji, Mohammad Javad Kharazifard
INTRODUCTION: This in vitro study compared the coronal microleakage of mineral trioxide aggregate (MTA), calcium-enriched mixture (CEM) cement and Biodentine as intra-orifice barriers. METHODS AND MATERIALS: The study was conducted on 76 extracted single-canal human teeth. Their root canals were prepared using ProTaper rotary files and filled with gutta percha and AH-26 sealer using lateral condensation technique. Coronal 3 mm of the gutta percha was removed from the root canals and replaced randomly with MTA, CEM cement or Biodentine in the three experimental groups (n=22)...
2017: Iranian Endodontic Journal
https://www.readbyqxmd.com/read/28512485/effect-of-blood-exposure-on-push-out-bond-strength-of-four-calcium-silicate-based-cements
#12
Kazem Ashofteh Yazdi, Behnam Bolhari, Tohid Sabetmoghaddam, Naghmeh Meraji, Mohammad Javad Kharazifard
INTRODUCTION: The purpose of this study was to compare the push-out bond strength of white ProRoot Mineral Trioxide Aggregate (MTA), Biodentine, calcium-enriched mixture (CEM) cement and Endosequence Root Repair Material (ERRM) putty after exposure to blood. METHODS AND MATERIALS: A total of 96 root dentin slices with a standardized thickness of 1.00±0.05 mm and standardized canal spaces were randomly divided into 4 main experimental groups (n=24) according to the calcium silicate based cement (CSC) used: white ProRoot MTA, CEM Cement, ERRM Putty and Biodentine...
2017: Iranian Endodontic Journal
https://www.readbyqxmd.com/read/28512479/in-vitro-cytotoxicity-comparison-of-mta-fillapex-ah-26-and-apatite-root-canal-sealer-at-different-setting-times
#13
Farnaz Jafari, Marzieh Aghazadeh, Sanaz Jafari, Faraz Khaki, Fahime Kabiri
INTRODUCTION: This study aimed to compare the cytotoxicity of MTA Fillapex, AH-26 and Apatite root canal sealers at different times after mixing. METHODS AND MATERIALS: In this in vitro study, MTA Fillapex, AH-26 and Apatite root canal sealer were spilled uniformly by 40 µm mesh in a 96-well plate. Then, human fetal foreskin fibroblast cell line (HFFF2) were added to each sealer cell culture medium. Cytotoxicity was measured using MTT assay after 24, 48 and 72 h and seven days...
2017: Iranian Endodontic Journal
https://www.readbyqxmd.com/read/28504319/biological-response-of-commercially-available-different-tricalcium-silicate-based-cements-and-pozzolan-cement
#14
Serhat Köseoğlu, Tuğba Pekbağr Yan K, Ebru Kucukyilmaz, Mehmet Sağlam, Sukru Enhos, Ayşe Akgün
This study evaluated four root repair materials for cytotoxicity and cell attachment in vitro. Cell viability was determined at 24 hr, 3 days and 7 days by using a 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay with material extracts. Cell adhesion was examined with a scanning electron microscope on the surface of materials at 24 and 48 hr. Angelus MTA displayed the lowest cell viabilities at all-day incubations. Endocem had high biocompatibility on the first day. After culturing for 3 days and 7 days, the cell viabilities of Biodentine, Endocem and MM-MTA had similar results, and their cell viability was significantly higher than that of Angelus...
May 15, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28493112/the-teaching-of-management-of-the-pulp-in-primary-molars-across-europe
#15
J Monteiro, A Ní Chaollaí, M Duggal
AIM: To determine which methods of primary pulp therapy are currently being taught in European dental schools. METHODS: An online survey tool was employed to send questionnaires to paediatric dentistry departments of 202 European dental schools. Email addresses were obtained from the European Academy of Paediatric Dentistry and questionnaires were sent to one member of each department in December 2014. The survey included questions on treatment choices and clinical scenarios where respondents indicated how they would advise students to proceed, given a particular case...
May 10, 2017: European Archives of Paediatric Dentistry: Official Journal of the European Academy of Paediatric Dentistry
https://www.readbyqxmd.com/read/28492745/cytotoxicity-evaluation-of-root-canal-sealers-using-an-in-vitro-experimental-model-with-roots
#16
Ligia Teixeira, Fernanda Gonçalves Basso, Josimeri Hebling, Carlos Alberto de Souza Costa, Graziela Garrido Mori, Yara Teresinha Corrêa Silva-Sousa, Camila Fávero de Oliveira
The aim of the present study was to evaluate the cytotoxicity of root canal sealers under conditions closely resembling a clinical reality. A primary human fibroblast cell line was seeded in 24-well acrylic plates with Dulbecco's modified Eagle's medium supplemented with 10% serum fetal bovine (SFB) and incubated for 24 h. Root canals from premolars were filled and individually attached to nylon devices to be stabilized in the wells with the already seeded cells. Specimens were divided into groups as follows: Control: gutta-percha cones (GPC); AH Plus+GPC; Sealapex+GPC; MTA Fillapex+GPC and Endofill+GPC...
March 2017: Brazilian Dental Journal
https://www.readbyqxmd.com/read/28489485/igf-1-mediates-ephrinb1-activation-in-regulating-tertiary-dentin-formation
#17
S Matsumura, A Quispe-Salcedo, C M Schiller, J S Shin, B M Locke, S Yakar, E Shimizu
Eph receptors belong to a subfamily of receptor tyrosine kinases that are activated by membrane-spanning ligands called ephrins. Previously, we demonstrated that the ephrinB1-EphB2 interaction regulates odontogenic/osteogenic differentiation from dental pulp cells (DPCs) in vitro. The goal of this study was to identify the molecular mechanisms regulated by the EphB2/ephrinB1 system that govern tertiary dentin formation in vitro and in vivo. During tooth development, ephrinB1, and EphB2 were expressed in preodontoblast and odontoblasts at postnatal day 4...
May 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28479918/bovine-tooth-discoloration-induced-by-endodontic-filling-materials-for-primary-teeth
#18
Samantha Rodrigues Xavier, Katerine Jahnecke Pilownic, Andressa Heberle Gastmann, Mariana Silveira Echeverria, Ana Regina Romano, Fernanda Geraldo Pappen
Objective. This study evaluated the discoloration potential of endodontic materials used in primary teeth. Material and Methods. Dentine-enamel blocks were prepared from 75 bovine teeth, assorted in five experimental groups (n = 15). The tested materials included an MTA-based material; zinc oxide and eugenol cement (ZOE); Vitapex; and calcium hydroxide thickened with zinc oxide (Calen + ZO). The color measurements were performed using a spectrophotometer at the following intervals: prior to (T0) and after placement of the filling (T1) and after 1 week (T2), 1 month (T3), 3 months (T4), 6 months (T5), and 9 months (T6)...
2017: International Journal of Dentistry
https://www.readbyqxmd.com/read/28476469/furcation-perforation-periradicular-tissue-response-to-biodentine-as-a-repair-material-by-histopathologic-and-indirect-immunofluorescence-analyses
#19
Lea Assed Bezerra Silva, Karina Alessandra Michelão Grecca Pieroni, Paulo Nelson-Filho, Raquel Assed Bezerra Silva, Patrícia Hernandéz-Gatón, Marília Pacífico Lucisano, Francisco Wanderley Garcia Paula-Silva, Alexandra Mussolino de Queiroz
INTRODUCTION: The purpose of this study was to evaluate the in vivo response of periradicular tissues after sealing of furcation perforations with Biodentine, mineral trioxide aggregate (MTA), and gutta-percha by means of histopathologic and indirect immunofluorescence analyses. METHODS: Thirty teeth of 3 dogs were divided into 3 groups: Biodentine (n = 14 teeth), MTA (negative control, n = 10 teeth), and gutta-percha (positive control, n = 6 teeth). After endodontic treatment, perforations were made on the center of the pulp chamber floor and filled with the materials...
May 2, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28469826/comparative-cytotoxicity-evaluation-of-eight-root-canal-sealers
#20
Claudio Poggio, Paolo Riva, Marco Chiesa, Marco Colombo, Giampiero Pietrocola
BACKGROUND: The aim of the present study is to evaluate and compare the cytotoxic effects of eight root canal sealers (BioRoot RCS, TotalFill BC Sealer, MTA Fillapex, Sealapex, AH Plus, EasySeal, Pulp Canal Sealer, N2) on immortalized human gingival fibroblasts over a period of 24, 48 and 72 hours. MATERIAL AND METHODS: Immortalized human gingival fibroblast-1 HGF-1 (ATCC CRL-2014) were incubated. Root canal sealers were then placed into sterile, cylindrical Teflon moulds...
April 2017: Journal of Clinical and Experimental Dentistry
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