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https://www.readbyqxmd.com/read/28229407/assessment-of-energy-metabolic-changes-in-adipose-tissue-derived-stem-cells
#1
Ghazaleh Hajmousa, Martin C Harmsen
Adipose tissue-derived stem cells (ADSC) are promising candidates for therapeutic applications in cardiovascular regenerative medicine. By definition, the phenotype ADSCs, e.g., the ubiquitous secretion of growth factors, cytokines, and extracellular matrix components is not met in vivo, which renders ADSC a culture "artefact." The medium constituents therefore impact the efficacy of ADSC. Little attention has been paid to the energy source in medium, i.e., glucose, which feeds the cell's power plants: mitochondria...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28220138/de-novo-transcriptome-assembly-of-phomopsis-liquidambari-provides-insights-into-genes-associated-with-different-lifestyles-in-rice-oryza-sativa-l
#2
Jun Zhou, Xin Li, Yan Chen, Chuan-Chao Dai
The mechanisms that trigger the switch from endophytic fungi to saprophytic fungi are largely unexplored. Broad host range Phomopsis liquidambari is established in endophytic and saprophytic systems with rice (Oryza sativa L.). Endophytic P. liquidambari promotes rice growth, increasing rice yield and improving the efficiency of nitrogen fertilizer. This species's saprophytic counterpart can decompose rice litterfall, promoting litter organic matter cycling and the release of nutrients and improving the soil microbial environment...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28197163/quinolizidine-alkaloid-biosynthesis-in-lupins-and-prospects-for-grain-quality-improvement
#3
REVIEW
Karen M Frick, Lars G Kamphuis, Kadambot H M Siddique, Karam B Singh, Rhonda C Foley
Quinolizidine alkaloids (QAs) are toxic secondary metabolites found within the genus Lupinus, some species of which are commercially important grain legume crops including Lupinus angustifolius (narrow-leafed lupin, NLL), L. luteus (yellow lupin), L. albus (white lupin), and L. mutabilis (pearl lupin), with NLL grain being the most largely produced of the four species in Australia and worldwide. While QAs offer the plants protection against insect pests, the accumulation of QAs in lupin grain complicates its use for food purposes as QA levels must remain below the industry threshold (0...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28194564/xylogenesis-in-zinnia-zinnia-elegans-cell-cultures-unravelling%C3%A2-the-regulatory-steps-in-a-complex-developmental-programmed-cell-death-event
#4
REVIEW
Elena T Iakimova, Ernst J Woltering
Physiological and molecular studies support the view that xylogenesis can largely be determined as a specific form of vacuolar programmed cell death (PCD). The studies in xylogenic zinnia cell culture have led to many breakthroughs in xylogenesis research and provided a background for investigations in other experimental models in vitro and in planta . This review discusses the most essential earlier and recent findings on the regulation of xylem elements differentiation and PCD in zinnia and other xylogenic systems...
February 13, 2017: Planta
https://www.readbyqxmd.com/read/28191957/towards-financially-viable-phytoextraction-and-production-of-plant-based-palladium-catalysts
#5
Zakuan Azizi Shamsul Harumain, Helen L Parker, Andrea Muñoz García, Michael J Austin, Con Robert McElroy, Andrew J Hunt, James H Clark, John A Meech, Christopher William Noel Anderson, Luca Ciacci, Thomas E Graedel, Neil C Bruce, Elizabeth L Rylott
Although a promising technique, phytoextraction has yet to see significant commercialization. Major limitations include metal uptake rates and subsequent processing costs. However, it has been shown that liquid-culture-grown Arabidopsis can take up and store palladium as nanoparticles. The processed plant biomass has catalytic activity comparable to that of commercially available catalysts, creating a product of higher value than extracted bulk metal. We demonstrate that the minimum level of palladium in Arabidopsis dried tissues for catalytic activity comparable to commercially available 3% palladium-on-carbon catalysts was achieved from dried plant biomass containing between 12 and 18 g·kg-1 Pd...
February 13, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28187422/differential-expression-of-hyperhydricity-responsive-peach-mirnas
#6
Ebru Diler, Turgay Unver, Gökhan Karakülah
Hyperhydricity is a syndrome that causes morpho-physiological malformations in tissue culture plantlets. Micro-RNAs (miRNA) are small non-coding RNAs that play important regulatory roles in plant development, stress response, and adaptation to environmental conditions. In this study, differential expression analysis indicated that miRNAs play an underlying role in the responses to the hyperhydricity syndrome in peach Prunus persica (L.) leaves. 24 known and three novel potential miRNAs were characterized in hyperhydric and non-hyperhydric transcriptome libraries...
December 31, 2016: Journal of Integrative Bioinformatics
https://www.readbyqxmd.com/read/28186422/seeds-of-the-wild-progenitor-of-maize-possess-bacteria-that-antagonize-foodborne-pathogens
#7
Hanan R Shehata, Mansel W Griffiths, Manish N Raizada
Endophytes are microorganisms that inhabit plant tissues without causing disease. Some endophytes help their hosts to combat pathogens. Here we explored the hypothesis that the plant-derived foods consumed by humans and other animals host endophytes that also antagonize foodborne pathogens or food-rotting agents. Our laboratory previously cultured a library of bacterial endophytes from different members of the maize/corn family (Zea) including wild relatives. Here, 190 of these endophytes were screened for their ability to antagonize four foodborne pathogens (Escherichia coli O157:H7, Listeria monocytogenes, Clostridium perfringens, and Salmonella enterica Newport) and a food spoiling agent (Pseudomonas fluorescens) using dual culture assays...
February 10, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28176760/dogmp1-from-dendrobium-officinale-contributes-to-mannose-content-of-water-soluble-polysaccharides-and-plays-a-role-in-salt-stress-response
#8
Chunmei He, Zhenming Yu, Jaime A Teixeira da Silva, Jianxia Zhang, Xuncheng Liu, Xiaojuan Wang, Xinhua Zhang, Songjun Zeng, Kunlin Wu, Jianwen Tan, Guohua Ma, Jianping Luo, Jun Duan
GDP-mannose pyrophosphorylase (GMP) catalyzed the formation of GDP-mannose, which serves as a donor for the biosynthesis of mannose-containing polysaccharides. In this study, three GMP genes from Dendrobium officinale (i.e., DoGMPs) were cloned and analyzed. The putative 1000 bp upstream regulatory region of these DoGMPs was isolated and cis-elements were identified, which indicates their possible role in responses to abiotic stresses. The DoGMP1 protein was shown to be localized in the cytoplasm. To further study the function of the DoGMP1 gene, 35S:DoGMP1 transgenic A...
February 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28176375/cryptotanshinone-inhibits-stat3-signaling-to-alleviate-cardiac-fibrosis-in-type-1-like-diabetic-rats
#9
Shih-Hsiang Lo, Chao-Tien Hsu, Ho-Shan Niu, Chiang-Shan Niu, Juei-Tang Cheng, Zhih-Cherng Chen
Cryptotanshinone is an active principal ingredient isolated from Salvia miltiorrhiza (Danshen), a medicinal plant used in China to treat cardiac disorders. The objective of this study was to investigate the effect of cryptotanshinone on myocardial fibrosis in diabetic rats. In streptozotocin-induced type 1 diabetic model hyperglycemic rats (STZ-treated rats), fasting blood glucose levels and heart weight/body weight ratio were markedly increased but both were not modified by cryptotanshinone. Additionally, cardiac performance in catheterized STZ-treated rats was improved...
February 8, 2017: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/28169241/enhanced-cytotoxic-activity-of-endophytic-bacterial-extracts-from-adhatoda-beddomei-leaves-in-a549-lung-cancer-cell-lines
#10
Y Swarnalatha, Bhaswti Saha
AIM OF THE STUDY: The current study is aimed to isolate and study the efficacy of the anticancer activity of endophytic bacteria from adhatoda beddomei leaves. Endophytic bacteria, microorganisms can found in the plant tissues, like leaves, branches and roots and able to produce various novel secondary metabolites for the medicinal applications. METHODOLOGY: Endophytic bacteria were isolated from the leaves of the adhatoda beddomei leaves. The extract from the culture was tested for the cytotoxicity in A549 cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)assay, dual staining and nuclear staining...
October 2016: Journal of Cancer Research and Therapeutics
https://www.readbyqxmd.com/read/28167891/construction-of-a-system-for-the-strawberry-nursery-production-towards-elimination-of-latent-infection-of-anthracnose-fungi-by-a-combination-of-pcr-and-microtube-hybridization
#11
Kazuyoshi Furuta, Saki Nagashima, Tsuyoshi Inukai, Chikara Masuta
One of the major problems in strawberry production is difficulty in diagnosis of anthracnose caused by Colletotrichum acutatum or Glomerella cingulata in latent infection stage. We here developed a diagnostic tool for the latent infection consisting of initial culturing of fungi, DNA extraction, synthesis of PCR-amplified probes and microtube hybridization (MTH) using a macroarray. The initial culturing step is convenient to lure the fungi out of the plant tissues, and to extract PCR-inhibitor-free DNA directly from fungal hyphae...
February 2017: Plant Pathology Journal
https://www.readbyqxmd.com/read/28167884/pathological-status-of-pyricularia-angulata-causing-blast-and-pitting-disease-of-banana-in-eastern-india
#12
Sangeetha Ganesan, Hari Shankar Singh, Srinivas Petikam, Debasish Biswal
Incidence of leaf blast on nursery plants and pitting disease on maturing banana bunches has been recorded in banana plantations during rainy season in Eastern India during 2014 to 2015. Taxonomical identification as well as DNA sequence analysis of the internal transcribed spacer region of fungus isolated from affected tissue culture derived plantlets and fruits confirmed the pathogen to be Pyricularia angulata Hashioka "in both the cases". Koch's postulates were proved on young plantlets as well as on maturing fruits of cv...
February 2017: Plant Pathology Journal
https://www.readbyqxmd.com/read/28161858/wheat-drought-responsive-wxpl-transcription-factors-regulate-cuticle-biosynthesis-genes
#13
Huihui Bi, Sukanya Luang, Yuan Li, Natalia Bazanova, Nikolai Borisjuk, Maria Hrmova, Sergiy Lopato
The cuticle forms a hydrophobic waxy layer that covers plant organs and provides protection from biotic and abiotic stresses. Transcription of genes responsible for cuticle formation is regulated by several types of transcription factors (TFs). Five orthologous to WAX PRODUCTION (WXP1 and WXP2) genes from Medicago truncatula were isolated from a cDNA library prepared from flag leaves and spikes of drought tolerant wheat (Triticum aestivum, breeding line RAC875) and designated TaWXP-like (TaWXPL) genes. Tissue-specific and drought-responsive expression of TaWXPL1D and TaWXPL2B was investigated by quantitative RT-PCR in two Australian wheat genotypes, RAC875 and Kukri, with contrasting glaucousness and drought tolerance...
February 4, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28160057/a-culture-independent-approach-to-enrich-endophytic-bacterial-cells-from-sugarcane-stems-for-community-characterization
#14
Carlos M Dos-Santos, Daniel G de Souza, Eduardo Balsanelli, Leonardo Magalhães Cruz, Emanuel M de Souza, José I Baldani, Stefan Schwab
Bacterial endophytes constitute a very diverse community and they confer important benefits which help to improve agricultural yield. Some of these benefits remain underexplored or little understood, mainly due to the bottlenecks associated with the plant feature, a low number of endophytic bacterial cells in relation to the plant, and difficulties in accessing these bacteria using cultivation-independent methods. Enriching endophytic bacterial cells from plant tissues, based on a non-biased, cultivation-independent physical enrichment method, may help to circumvent those problems, especially in the case of sugarcane stems, which have a high degree of interfering factors, such as polysaccharides, phenolic compounds, nucleases, and fibers...
February 3, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28142310/antioxidant-properties-of-ferulago-angulata-and-its-hepatoprotective-effect-against-n-nitrosodimethylamine-induced-oxidative-stress-in-rats
#15
Hatice Kiziltas, Suat Ekin, Mahire Bayramoglu, Esvet Akbas, Gokhan Oto, Serkan Yildirim, Fevzi Ozgokce
CONTEXT: Ferulago angulata (Schlecht.) Boiss. (Apiaceae) (FASB) is used to treat liver diseases and has been used both as food and therapeutics by many cultures for thousands of years because of the natural antioxidant compounds. OBJECTIVE: This study determines antioxidant properties of FASB flowers, the levels of minerals and vitamins, and also, evaluates the hepatoprotective effect of flowers against N-nitrosodimethylamine (NDMA) induced on liver tissue by assessing antioxidant enzymes and histopathological parameters in Wistar albino rats...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28141854/soybean-cyst-nematode-culture-collections-and-field-populations-from-north-carolina-and-missouri-reveal-high-incidences-of-infection-by-viruses
#16
Casey L Ruark, Stephen R Koenning, Eric L Davis, Charles H Opperman, Steven A Lommel, Melissa G Mitchum, Tim L Sit
Five viruses were previously discovered infecting soybean cyst nematodes (SCN; Heterodera glycines) from greenhouse cultures maintained in Illinois. In this study, the five viruses [ScNV, ScPV, ScRV, ScTV, and SbCNV-5] were detected within SCN greenhouse and field populations from North Carolina (NC) and Missouri (MO). The prevalence and titers of viruses in SCN from 43 greenhouse cultures and 25 field populations were analyzed using qRT-PCR. Viral titers within SCN greenhouse cultures were similar throughout juvenile development, and the presence of viral anti-genomic RNAs within egg, second-stage juvenile (J2), and pooled J3 and J4 stages suggests active viral replication within the nematode...
2017: PloS One
https://www.readbyqxmd.com/read/28138240/plant-derived-pectin-nanocoatings-to-prevent-inflammatory-cellular-response-of-osteoblasts-following-porphyromonas-gingivalis-infection
#17
Anna Meresta, Justyna Folkert, Timo Gaber, Korneliusz Miksch, Frank Buttgereit, Jacqueline Detert, Nicole Pischon, Katarzyna Gurzawska
BACKGROUND: Bioengineered plant-derived Rhamnogalacturonan-Is (RG-Is) from pectins are potential candidates for surface nanocoating of medical devices. It has recently been reported that RG-I nanocoatings may prevent bacterial infection and improve the biocompatibility of implants. The aim of the study was to evaluate in vitro impact of bioengineered RG-I nanocoatings on osteogenic capacity and proinflammatory cytokine response of murine osteoblasts following Porphyromonas gingivalis infection...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28132143/manipulation-of-oat-protoplasts-for-transient-expression-assays
#18
Robyn Roberts, Jincan Zhang, Nicole Mihelich, Danielle Savino, Aurélie M Rakotondrafara
Oat protoplasts are a useful and convenient system to study transient expression using whole cells. Nucleic acid can rapidly be introduced into live cells, and, depending on the assay, results can be collected the same day. Compared to plant tissue, oat cell suspension cultures provide a simple, high yielding, and consistent means to isolate protoplasts. Here, we describe how to generate an oat cell suspension culture from callus grown on solidified medium, and how to maintain the oat cells in cell suspension culture for protoplast preparation...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28124776/colonization-study-of-gfp-tagged-achromobacter-marplatensis-strain-in-sugar-beet
#19
YingWu Shi, Chun Li, HongMei Yang, Tao Zhang, Yan Gao, Min Chu, Jun Zeng, Qing Lin, OuTiKuEr, YuGuo Li, Xiangdong Huo, Kai Lou
This study details the introduction of a gfp marker into an endophytic bacterial strain (Achromobacter marplatensis strain 17, isolated from sugar beet) to monitor its colonization of sugar beet (Beta. vulgaris L.). Stability of the plasmid encoding the gfp was confirmed in vitro for at least 72 h of bacterial growth and after the colonization of tissues, under nonselective conditions. The colonization was observed using fluorescence microscopy and enumeration of culturable endophytes in inoculated sugar beet plants that grew for 10 or 20 days...
January 26, 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28124026/effects-of-exogenous-yeast-and-bacteria-on-the-microbial-population-dynamics-and-outcomes-of-olive-fermentations
#20
Jose Zaragoza, Zachary Bendiks, Charlotte Tyler, Mary E Kable, Thomas R Williams, Yelizaveta Luchkovska, Elaine Chow, Kyria Boundy-Mills, Maria L Marco
In this study, we examined Sicilian-style green olive fermentations upon the addition of Saccharomyces cerevisiae UCDFST 09-448 and/or Pichia kudriazevii UCDFST09-427 or the lactic acid bacteria (LAB) Lactobacillus plantarum AJ11R and Leuconostoc pseudomesenteroides BGM3R. Olives containing S. cerevisiae UCDFST 09-448, a strain able to hydrolyze pectin, but not P. kudriazevii UCDFST 09-427, a nonpectinolytic strain, exhibited excessive tissue damage within 4 weeks. DNA sequencing of fungal internal transcribed spacer (ITS) regions and comparisons to a yeast-specific ITS sequence database remarkably showed that neither S...
January 2017: MSphere
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