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https://www.readbyqxmd.com/read/27909432/volatile-mediated-effects-predominate-in-paraburkholderia-phytofirmans-growth-promotion-and-salt-stress-tolerance-of-arabidopsis-thaliana
#1
Thomas Ledger, Sandy Rojas, Tania Timmermann, Ignacio Pinedo, María J Poupin, Tatiana Garrido, Pablo Richter, Javier Tamayo, Raúl Donoso
Abiotic stress has a growing impact on plant growth and agricultural activity worldwide. Specific plant growth promoting rhizobacteria have been reported to stimulate growth and tolerance to abiotic stress in plants, and molecular mechanisms like phytohormone synthesis and 1-aminocyclopropane-1-carboxylate deamination are usual candidates proposed to mediate these bacterial effects. Paraburkholderia phytofirmans PsJN is able to promote growth of several plant hosts, and improve their tolerance to chilling, drought and salinity...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27899931/melatonin-in-plants-and-plant-culture-systems-variability-stability-and-efficient-quantification
#2
Lauren A E Erland, Abhishek Chattopadhyay, Andrew Maxwell P Jones, Praveen K Saxena
Despite growing evidence of the importance of melatonin and serotonin in the plant life, there is still much debate over the stability of melatonin, with extraction and analysis methods varying greatly from lab to lab with respect to time, temperature, light levels, extraction solvents, and mechanical disruption. The variability in methodology has created conflicting results that confound the comparison of studies to determine the role of melatonin in plant physiology. We here describe a fully validated method for the quantification of melatonin, serotonin and their biosynthetic precursors: tryptophan, tryptamine and N-acetylserotonin by liquid chromatography single quadrupole mass spectrometry (LC-MS) in diverse plant species and tissues...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27898837/global-transcriptome-analysis-reveals-differences-in-gene-expression-patterns-between-nonhyperhydric-and-hyperhydric-peach-leaves
#3
Yakup Bakir, Vahap Eldem, Gokmen Zararsiz, Turgay Unver
Hyperhydricity is a morphophysiological disorder of plants in tissue culture characterized morphologically by the presence of translucent, thick, curled, and fragile leaves as a result of excessive water intake. Since clonal propagation is a major in vitro technique for multiplying plants vegetatively, the emergence of hyperhydricity-related symptoms causes significant economic losses to agriculture and horticulture. Although numerous efforts have been hitherto devoted to the morphological and anatomical responses of plants to hyperhydricity, the underlying molecular mechanism remains largely unknown...
July 2016: Plant Genome
https://www.readbyqxmd.com/read/27864277/molecular-characterization-of-ghpld%C3%AE-1-and-its-relationship-with-secondary-cell-wall-thickening-in-cotton-fibers
#4
Kai Tang, Jin-Yuan Liu
Phospholipase D (PLD) hydrolyzes phospholipids to generate a free polar head group (e.g., choline) and a second messenger phosphatidic acid and plays diverse roles in plant growth and development, including seed germination, leaf senescence, root hair growth, and hypocotyl elongation. However, the function of PLD in cotton remains largely unexplored. Here, the comprehensive molecular characterization of GhPLDα1 was explored with its role in upland cotton (Gossypium hirsutum) fiber development. The GhPLDα1 gene was cloned successfully, and a sequence alignment showed that GhPLDα1 contains one C2 domain and two HKD (HxKxxxxD) domains...
November 17, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27853450/antifungal-streptomyces-spp-associated-with-the-infructescences-of-protea-spp-in-south-africa
#5
Zander R Human, Kyuho Moon, Munhyung Bae, Z Wilhelm de Beer, Sangwon Cha, Michael J Wingfield, Bernard Slippers, Dong-Chan Oh, Stephanus N Venter
Common saprophytic fungi are seldom present in Protea infructescences, which is strange given the abundance of mainly dead plant tissue in this moist protected environment. We hypothesized that the absence of common saprophytic fungi in Protea infructescences could be due to a special symbiosis where the presence of microbes producing antifungal compounds protect the infructescence. Using a culture based survey, employing selective media and in vitro antifungal assays, we isolated antibiotic producing actinomycetes from infructescences of Protea repens and P...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27853060/physical-chemical-and-proteomic-evidence-of-potato-suberin-degradation-by-the-plant-pathogenic-bacterium-streptomyces-scabiei
#6
Carole Beaulieu, Amadou Sidibé, Raoudha Jabloune, Anne-Marie Simao-Beaunoir, Sylvain Lerat, Ernest Monga, Mark A Bernards
Potato peels consist of a tissue called phellem, which is formed by suberized cell layers. The degradation of suberin, a lipidic and recalcitrant polymer, is an ecological process attributed to soil fungal populations; however, previous studies have suggested that Streptomyces scabiei, the causal agent of potato common scab, possesses the ability to degrade suberin. In the present study, S. scabiei was grown in medium containing suberin-enriched potato phellem as the sole carbon source and its secretome was analyzed periodically (10- to 60-d-old cultures) with a special focus on proteins potentially involved in cell wall degradation...
November 17, 2016: Microbes and Environments
https://www.readbyqxmd.com/read/27852870/biological-activities-of-extracellular-vesicles-and-their-cargos-from-bovine-and-human-milk-in-humans-and-implications-for-infants
#7
REVIEW
Janos Zempleni, Ana Aguilar-Lozano, Mahrou Sadri, Sonal Sukreet, Sonia Manca, Di Wu, Fang Zhou, Ezra Mutai
Extracellular vesicles (EVs) in milk harbor a variety of compounds, including lipids, proteins, noncoding RNAs, and mRNAs. Among the various classes of EVs, exosomes are of particular interest, because cargo sorting in exosomes is a regulated, nonrandom process and exosomes play essential roles in cell-to-cell communication. Encapsulation in exosomes confers protection against enzymatic and nonenzymatic degradation of cargos and provides a pathway for cellular uptake of cargos by endocytosis of exosomes. Compelling evidence suggests that exosomes in bovine milk are transported by intestinal cells, vascular endothelial cells, and macrophages in human and rodent cell cultures, and bovine-milk exosomes are delivered to peripheral tissues in mice...
November 16, 2016: Journal of Nutrition
https://www.readbyqxmd.com/read/27843018/phytotoxicity-uptake-and-transformation-of-nano-ceo2-in-sand-cultured-romaine-lettuce
#8
Peng Zhang, Yuhui Ma, Shutong Liu, Guohua Wang, Junzhe Zhang, Xiao He, Jing Zhang, Yukui Rui, Zhiyong Zhang
Toxicity and uptake of nano-CeO2 (nCeO2) in edible vegetables are not yet fully understood. In the present study, we grew romaine lettuce in sand amended with nCeO2. At high concentrations (1000 and 2000 mg/kg), nCeO2 diminished the chlorophyll content by 16.5% and 25.8%, respectively, and significantly inhibited the biomass production. nCeO2 (≥100 mg/kg) altered antioxidant enzymatic activities and malondialdehyde levels in the plants. nCeO2 (≥500 mg/kg) triggered a remarkable increase of nitrate-N level in the shoots, which can be converted to toxic nitrite in humans thereby posed risk to human health...
November 11, 2016: Environmental Pollution
https://www.readbyqxmd.com/read/27837372/development-of-newly-sanguinarine-biosynthetic-capacity-in-in-vitro-rootless-shoots-of-argemone-mexicana-l-mexican-prickly-poppy
#9
Jorge Xool-Tamayo, Germán Serrano-Gamboa, Miriam Monforte-González, Gumersindo Mirón-López, Felipe Vázquez-Flota
OBJECTIVE: To analyze berberine and sanguinarine biosynthetic capacities of both in vitro shoot and root cultures of Argemone mexicana and tissues from entire plants at different developmental stages. RESULTS: Berberine and sanguinarine were equally distributed in roots and aerial tissues of developing plantlet whereas, in juvenile plants, sanguinarine was only detected in roots. This alkaloid distribution was consistent with that of biosynthetic transcripts in juvenile plants...
November 11, 2016: Biotechnology Letters
https://www.readbyqxmd.com/read/27837351/biomanufacturing-history-and-perspective
#10
REVIEW
Yi-Heng Percival Zhang, Jibin Sun, Yanhe Ma
Biomanufacturing is a type of manufacturing that utilizes biological systems (e.g., living microorganisms, resting cells, animal cells, plant cells, tissues, enzymes, or in vitro synthetic (enzymatic) systems) to produce commercially important biomolecules for use in the agricultural, food, material, energy, and pharmaceutical industries. History of biomanufacturing could be classified into the three revolutions in terms of respective product types (mainly), production platforms, and research technologies...
November 11, 2016: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27833912/sacb-sacr-gene-cassette-as-the-negative-selection-marker-to-suppress-agrobacterium-overgrowth-in-agrobacterium-mediated-plant-transformation
#11
Yiming Liu, Jiamin Miao, Sy Traore, Danyu Kong, Yi Liu, Xunzhong Zhang, Zachary L Nimchuk, Zongrang Liu, Bingyu Zhao
Agrobacterium overgrowth is a common problem in Agrobacterium-mediated plant transformation. To suppress the Agrobacterium overgrowth, various antibiotics have been used during plant tissue culture steps. The antibiotics are expensive and may adversely affect plant cell differentiation and reduce plant transformation efficiency. The SacB-SacR proteins are toxic to most Agrobacterium tumefaciens strains when they are grown on culture medium supplemented with sucrose. Therefore, SacB-SacR genes can be used as negative selection markers to suppress the overgrowth of A...
2016: Frontiers in Molecular Biosciences
https://www.readbyqxmd.com/read/27833633/occurrence-and-distribution-of-phytochemicals-in-the-leaves-of-17-in-vitro-cultured-hypericum-spp-adapted-to-outdoor-conditions
#12
Andrea Kucharíková, Souvik Kusari, Selahaddin Sezgin, Michael Spiteller, Eva Čellárová
A plethora of plants belonging to the genus Hypericum have been investigated so far owing to the biological efficacies of pharmacologically important secondary metabolites produced by several Hypericum species. However, there is currently a dearth of information about the localization (accumulation) of these compounds in the plants in situ. In particular, the biosynthetic and ecological consequence of acclimatization of in vitro cultured Hypericum spp. to outdoor conditions is not fully known. Herein, we report an application of matrix-assisted laser desorption/ionization high-resolution mass spectrometry (MALDI-HRMS) to reveal the distribution of major naphthodianthrones hypericin, pseudohypericin, protohypericin, and their proposed precursor emodin as well as emodin anthrone, along with the phloroglucinol derivative hyperforin, the flavonoids quercetin, quercitrin, rutin and hyperoside (and/or isoquercitrin), and chlorogenic acid in Hypericum leaves...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27833620/camp-signaling-regulates-synchronised-growth-of-symbiotic-epichlo%C3%A3-fungi-with-the-host-grass-lolium-perenne
#13
Christine R Voisey, Michael T Christensen, Linda J Johnson, Natasha T Forester, Milan Gagic, Gregory T Bryan, Wayne R Simpson, Damien J Fleetwood, Stuart D Card, John P Koolaard, Paul H Maclean, Richard D Johnson
The seed-transmitted fungal symbiont, Epichloë festucae, colonizes grasses by infecting host tissues as they form on the shoot apical meristem (SAM) of the seedling. How this fungus accommodates the complexities of plant development to successfully colonize the leaves and inflorescences is unclear. Since adenosine 3', 5'-cyclic monophosphate (cAMP)-dependent signaling is often essential for host colonization by fungal pathogens, we disrupted the cAMP cascade by insertional mutagenesis of the E. festucae adenylate cyclase gene (acyA)...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27826796/bioengineering-of-the-plant-culture-of-capsicum-frutescens-with-vanillin-synthase-gene-for-the-production-of-vanillin
#14
Marcus Jenn Yang Chee, Grantley W Lycett, Teng-Jin Khoo, Chiew Foan Chin
Production of vanillin by bioengineering has gained popularity due to consumer demand toward vanillin produced by biological systems. Natural vanillin from vanilla beans is very expensive to produce compared to its synthetic counterpart. Current bioengineering works mainly involve microbial biotechnology. Therefore, alternative means to the current approaches are constantly being explored. This work describes the use of vanillin synthase (VpVAN), to bioconvert ferulic acid to vanillin in a plant system. The VpVAN enzyme had been shown to directly convert ferulic acid and its glucoside into vanillin and its glucoside, respectively...
November 8, 2016: Molecular Biotechnology
https://www.readbyqxmd.com/read/27825487/phytohormone-production-endowed-with-antagonistic-potential-and-plant-growth-promoting-abilities-of-culturable-endophytic-bacteria-isolated-from-clerodendrum-colebrookianum-walp
#15
Ajit Kumar Passari, Vineet Kumar Mishra, Vincent Vineeth Leo, Vijai Kumar Gupta, Bhim Pratap Singh
In this study, culturable endophytic bacterial isolates obtained from an ethnomedicinal plant Clerodendrum colebrookianum Walp., were assessed for their diversity, in vitro screening for their plant growth promoting (PGP) activities and to use them as inoculant for in vivo PGP activities with biocontrol potential. Totally, 73 isolates were recovered from different tissues of C. colebrookianum were identified by 16S rRNA gene sequencing and phylogenetically analyzed by using BOX-PCR fingerprinting. Out of 73 isolates, 52 exhibited varying extents of antagonistic potential were selected for screening for various PGP traits...
December 2016: Microbiological Research
https://www.readbyqxmd.com/read/27818605/the-potential-of-using-biotechnology-to-improve-cassava-a-review
#16
REVIEW
Paul Chavarriaga-Aguirre, Alejandro Brand, Adriana Medina, Mónica Prías, Roosevelt Escobar, Juan Martinez, Paula Díaz, Camilo López, Willy M Roca, Joe Tohme
The importance of cassava as the fourth largest source of calories in the world requires that contributions of biotechnology to improving this crop, advances and current challenges, be periodically reviewed. Plant biotechnology offers a wide range of opportunities that can help cassava become a better crop for a constantly changing world. We therefore review the state of knowledge on the current use of biotechnology applied to cassava cultivars and its implications for breeding the crop into the future. The history of the development of the first transgenic cassava plant serves as the basis to explore molecular aspects of somatic embryogenesis and friable embryogenic callus production...
2016: In Vitro Cellular & Developmental Biology. Plant: Journal of the Tissue Culture Association
https://www.readbyqxmd.com/read/27816659/ethnopharmacological-appraisal-of-culturally-important-medicinal-plants-and-polyherbal-formulas-used-against-communicable-diseases-in-rodrigues-island
#17
Anne Kathie Samoisy, Fawzi Mahomoodally
ETHNOPHARMACOLOGICAL RELEVANCE: The tropical island of Rodrigues is one of three islands that constitute the Mascarene archipelago in the Indian Ocean. Currently considered as a biodiversity hotspot, the island has also a resilient history in the use of traditional medicine. However, there has been no ethnopharmacological study to document quantitatively such traditional practice against communicable diseases (CD), which currently accounts for 6.61% annual death and 30.7% attendances in public hospitals...
November 2, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27815909/detection-of-changes-in-the-medicago-sativa-retinoblastoma-related-protein-msrbr1-phosphorylation-during-cell-cycle-progression-in-synchronized-cell-suspension-culture
#18
Ferhan Ayaydin, Edit Kotogány, Edit Ábrahám, Gábor V Horváth
Deepening our knowledge on the regulation of the plant cell division cycle depends on techniques that allow for the enrichment of cell populations in defined cell cycle phases. Synchronization of cell division can be achieved using different plant tissues; however, well-established cell suspension cultures provide large amount of biological sample for further analyses. Here, we describe the methodology of the establishment, propagation, and analysis of a Medicago sativa suspension culture that can be used for efficient synchronization of the cell division...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27812549/production-of-virus-free-orchid-cymbidium-aloifolium-l-sw-by-various-tissue-culture-techniques
#19
Shreeti Pradhan, Tripti Regmi, Mukunda Ranjit, Bijaya Pant
Orchids are affected by many viruses resulting in poor growth, yield and quality, and an overall decline in population. Cymbidium mosaic virus (CymMV) is one of the common orchid viruses found in Cymbidium species but it infects different orchid genera. In this study Cymbidium aloifolium was propagated in vitro using MS medium at different strength (1.0, ½, and ¼) with or without 0.5 mg/l BAP (6-benzylaminopurine) and 0.5 mg/l NAA (Naphthalene acetic acid). To provide disease-free planting material, source plant for in vitro propagation needs to be screened for pathogenic viruses...
October 2016: Heliyon
https://www.readbyqxmd.com/read/27807644/differential-proteome-analysis-during-early-somatic-embryogenesis-in-musa-spp-aaa-cv-grand-naine
#20
Marimuthu Kumaravel, Subbaraya Uma, Suthanthiram Backiyarani, Marimuthu Somasundaram Saraswathi, Muthu Mayil Vaganan, Muthusamy Muthusamy, Kallu Purayil Sajith
Endogenous hormone secretion proteins along with stress and defense proteins play predominant role in banana embryogenesis. This study reveals the underlying molecular mechanism during transition from vegetative to embryogenic state. Banana (Musa spp.) is well known globally as a food fruit crop for millions. The requirement of quality planting material of banana is enormous. Although mass multiplication through tissue culture is in vogue, high-throughput techniques like somatic embryogenesis (SE) as a mass multiplication tool needs to be improved...
November 2, 2016: Plant Cell Reports
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