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https://www.readbyqxmd.com/read/28651306/heme-oxygenase-1-induction-and-anti-inflammatory-actions-of-atractylodes-macrocephala-and-taraxacum-herba-extracts-prevented-colitis-and-was-more-effective-than-sulfasalazine-in-preventing-relapse
#1
Kyu-Hyun Han, Jong-Min Park, Migyeong Jeong, Young-Min Han, Eun-Jin Go, Juyeon Park, Hocheol Kim, Jae Gab Han, Oran Kwon, Ki Baik Hahm
Background/Aims: In inflammatory bowel disease (IBD), repeated bouts of remission and relapse occur in patients and can impose a risk of colitis-associated cancer. We hypothesized that plant extracts of Atractylodes macrocephala (AM) or Taraxacum herba (TH) may be better than sulfasalazine for treating this disease because these extracts can promote additional regeneration. Methods: Murine intestinal epithelial IEC-6 cells were pretreated with AM or TH before a lipopolysaccharide (LPS)-induced challenge...
June 27, 2017: Gut and Liver
https://www.readbyqxmd.com/read/28647832/genetic-dissection-of-adventitious-shoot-regeneration-in-roses-by-employing-genome-wide-association-studies
#2
Thi Hong Nhung Nguyen, Dietmar Schulz, Traud Winkelmann, Thomas Debener
We analysed the capacity to regenerate adventitious shoots in 96 rose genotypes and found 88 SNP markers associated with QTLs, some of which are derived from candidate genes for shoot regeneration. In an association panel of 96 rose genotypes previously analysed for petal colour, we conducted a genome-wide association study on the capacity of leaf petioles for direct shoot regeneration. Shoot regeneration rate and shoot ratio (number of shoots/total number of explants) were used as phenotypic descriptors for regeneration capacity...
June 24, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28640983/multiplexed-crispr-cas9-mediated-metabolic-engineering-of-%C3%AE-aminobutyric-acid-levels-in-solanum-lycopersicum
#3
Rui Li, Ran Li, Xindi Li, Daqi Fu, Benzhong Zhu, Huiqin Tian, Yunbo Luo, Hongliang Zhu
In recent years, the type II CRISPR system has become a widely used and robust technique to implement site-directed mutagenesis in a variety of species including model and crop plants. However, few studies manipulated metabolic pathways in plants using the CRISPR system. Here, we introduced the pYLCRISPR/Cas9 system with one or two single-site guide RNAs to target the tomato phytoene desaturase gene. An obvious albino phenotype was observed in T0 regenerated plants, and more than 61% of the desired target sites were edited...
June 22, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28623383/ethnopharmacological-uses-phytochemistry-biological-activities-and-biotechnological-applications-of-eclipta-prostrata
#4
REVIEW
Ill-Min Chung, Govindasamy Rajakumar, Ji-Hee Lee, Seung-Hyun Kim, Muthu Thiruvengadam
Eclipta prostrata belongs to a family of medicinal plants (Asteraceae) and plays a role in the treatment of several diseases, including infectious hepatitis, snake venom poisoning, gastritis, and respiratory diseases such as a cough and asthma. A number of compounds, including thiophene derivatives, steroids, triterpenes, flavonoids, polyacetylenes, polypeptides, and coumestans, have been isolated from E. prostrata. The plant functional compounds can act as reducing agent in the field of nanoparticle synthesis...
July 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28613372/cleaning-cassava-genotypes-infected-with-cassava-frogskin-disease-via-in-vitro-shoot-tip-culture
#5
M J S Carvalho, E J Oliveira, A S Souza, J S Pereira, M S A S Diamantino, S A S Oliveira
This study aimed to develop a methodology for eliminating cassava frogskin disease (CFSD) from in vitro shoot tip culture by associating thermotherapy and tetracycline. Cuttings from different accessions (BGM0232, BGM0315, BGM0464, BGM584, BGM0841, and BGM1342), infected with CFSD according to visual inspection of the disease symptoms, were used for cleaning. To verify the absence of other diseases, the plants were indexed for Cassava common mosaic virus - CsCMV (by ELISA) and Cassava vein mosaic virus - CsVMV (by polymerase chain reaction, PCR), proving that the accessions were free of these viruses, except for BGM0315 and BGM0464, which were infected with CsVMV...
May 31, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28606145/three-steps-in-one-pot-biosynthesis-of-4-hydroxycinnamyl-alcohols-using-immobilized-whole-cells-of-two-genetically-engineered-escherichia-coli-strains
#6
Shuxin Liu, Jiabin Liu, Jiayin Hou, Nan Chao, Ying Gai, Xiangning Jiang
BACKGROUND: 4-Hydroxycinnamyl alcohols are a class of natural plant secondary metabolites that include p-coumaryl alcohol, caffeyl alcohol, coniferyl alcohol and sinapyl alcohol, and have physiological, ecological and biomedical significance. While it is necessary to investigate the biological pathways and economic value of these alcohols, research is hindered because of their limited availability and high cost. Traditionally, these alcohols are obtained by chemical synthesis and plant extraction...
June 12, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28604361/synthetic-scaffolds-with-full-pore-interconnectivity-for-bone-regeneration-prepared-by-supercritical-foaming-using-advanced-biofunctional-plasticizers
#7
Aurelio Salerno, Sara Diéguez, Luis Diaz-Gomez, Jose Luis Gomez-Amoza, Beatriz Magariños, Angel Concheiro, Concepcion Domingo, Carmen Alvarez-Lorenzo, Carlos A García-González
Supercritical foaming allows for the solvent-free processing of synthetic scaffolds for bone regeneration. However, the control on the pore interconnectivity and throat pore size with this technique still needs to be improved. The use of plasticizers may help to overcome these limitations. Eugenol, a GRAS natural compound extracted from plants, is proposed in this work as an advanced plasticizer with bioactive properties. Eugenol-containing poly(ɛ-caprolactone) (PCL) scaffolds were obtained by supercritical foaming (20...
June 12, 2017: Biofabrication
https://www.readbyqxmd.com/read/28601354/studies-on-wound-healing-potential-of-polyherbal-formulation-using-in%C3%A2-vitro-and-in%C3%A2-vivo-assays
#8
Yogesh P Talekar, Kishori G Apte, Shubhangi V Paygude, Prasad R Tondare, Pradeep B Parab
BACKGROUND: The use of herbal plant extracts in wound healing is known through decades, but it is necessary to provide scientific data through reverse pharmacology. OBJECTIVE: The aim of the present study is to find the mechanism behind the healing of wounds using in vitro and in vivo assays. METHODS AND MATERIAL: The study was designed to determine proliferation and mobilization of fibroblast and keratinocytes at the site of injury, angiogenesis at the site of healing and reduction in oxidative stress while healing...
June 7, 2017: Journal of Ayurveda and Integrative Medicine
https://www.readbyqxmd.com/read/28597418/influence-of-led-light-spectra-on-in-vitro-somatic-embryogenesis-and-lc-ms-analysis-of-chlorogenic-acid-and-rutin-in-peucedanum-japonicum-thunb-a-medicinal-herb
#9
Chia-Chen Chen, Dinesh Chandra Agrawal, Maw-Rong Lee, Ren-Jye Lee, Chao-Lin Kuo, Chi-Rei Wu, Hsin-Sheng Tsay, Hung-Chi Chang
BACKGROUND: Peucedanum japonicum Thunb, an important medicinal herb is reported to possess pharmacological properties such as anti-obesity, anti-oxidant, anti-inflammatory, anti-bacterial, anti-diabetic and anti-platelet aggregation. The present study aimed to develop an in vitro plant regeneration system of P. japonicum via somatic embryogenesis and to analyse chlorogenic acid and rutin contents in a few commercially available plant products of P. japonicum in Japan and Taiwan markets, and tissue culture plants derived from somatic embryos...
December 2016: Botanical Studies (Taipei, Taiwan)
https://www.readbyqxmd.com/read/28587679/somatic-cell-selection-for-chlorsulfuron-resistant-mutants-in-potato-identification-of-point-mutations-in-the-acetohydroxyacid-synthase-gene
#10
Philippa J Barrell, Julie M Latimer, Samantha J Baldwin, Michelle L Thompson, Jeanne M E Jacobs, Anthony J Conner
BACKGROUND: Somatic cell selection in plants allows the recovery of spontaneous mutants from cell cultures. When coupled with the regeneration of plants it allows an effective approach for the recovery of novel traits in plants. This study undertook somatic cell selection in the potato (Solanum tuberosum L.) cultivar 'Iwa' using the sulfonylurea herbicide, chlorsulfuron, as a positive selection agent. RESULTS: Following 5 days' exposure of potato cell suspension cultures to 20 μg/l chlorsulfuron, rescue selection recovered rare potato cell colonies at a frequency of approximately one event in 2...
June 6, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28578707/meristem-culture-and-subsequent-micropropagation-of-chilean-strawberry-fragaria-chiloensis-l-duch
#11
Karla A Quiroz, Miguel Berríos, Basilio Carrasco, Jorge B Retamales, Peter D S Caligari, Rolando García-Gonzáles
BACKGROUND: Vegetative propagation of Fragaria sp. is traditionally carried out using stolons. This system of propagation, in addition to being slow, can spread plant diseases, particularly serious being viral. In vitro culture of meristems and the establishment of micropropagation protocols are important tools for solving these problems. In recent years, considerable effort has been made to develop in vitro propagation of the commercial strawberry in order to produce virus-free plants of high quality...
June 2, 2017: Biological Research
https://www.readbyqxmd.com/read/28576846/type-b-arabidopsis-response-regulators-is-critical-to-the-specification-of-shoot-stem-cell-niche-by-dual-regulation-of-wuschel
#12
Wenjing Meng, Zhi Juan Cheng, Ya Lin Sang, Miao Miao Zhang, Xiao Fei Rong, Zhi Wei Wang, Ying Ying Tang, Xian Sheng Zhang
Plants are known for their capacity to regenerate the whole body through de novo formation of apical meristems from a mass of proliferating cells named callus. Exogenous cytokinin and auxin determine cell fate for the establishment of the stem cell niche, which is the vital step of shoot regeneration, but the underlying mechanisms remain unclear. Here we show that type-B ARABIDOPSIS RESPONSE REGULATORs (ARRs), critical components of cytokinin signaling, activate the transcription of WUSCHEL (WUS), which encodes a key regulator for maintaining stem cells...
June 2, 2017: Plant Cell
https://www.readbyqxmd.com/read/28573234/evaluation-of-antibacterial-antitumor-antioxidant-activities-and-phenolic-constituents-of-field-grown-and-in-vitro-grown-lysimachia-vulgaris-l
#13
Arzu Birinci Yildirim, Birgul Guner, Fatma Pehlivan Karakas, Arzu Ucar Turker
BACKGROUND: Lysimachia vulgaris L. (Yellow loosestrife) is a medicinal plant in the family Myrsinaceae. It has been used in the treatment of fever, ulcer, diarrhea and wounds in folk medicine. It has also analgesic, expectorant, astringent and anti-inflammatory activities. Two different sources of the plant (field-grown and in vitro-grown) were used to evaluate the biological activities (antibacterial, antitumor and antioxidant) of L. vulgaris. In vitro-grown plant materials were collected from L...
2017: African Journal of Traditional, Complementary, and Alternative Medicines: AJTCAM
https://www.readbyqxmd.com/read/28572673/simple-rapid-and-efficient-transformation-of-genotype-nisqually-1-a-basic-tool-for-the-first-sequenced-model-tree
#14
Shujuan Li, Cheng Zhen, Wenjing Xu, Chong Wang, Yuxiang Cheng
Genotype Nisqually-1 is the first model woody plant with an available well-annotated genome. Nevertheless, a simple and rapid transformation of Nisqually-1 remains to be established. Here, we developed a novel shoot regeneration method for Nisqually-1 using leaf petiole and stem segment explants. Numerous shoots formed in the incision of explants within two weeks. The optimized shoot regeneration medium (SRM) contained 0.03 mg l(-1) 6-benzylaminopurine, 0.02 mg l(-1) indole-3-butyric acid and 0.0008 mg l(-1) thidiazuron...
June 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28567635/in-vitro-regeneration-of-drosera-burmannii-vahl-a-carnivorous-plant-of-north-east-india
#15
J Sureni Yanthan, Mechuselie Kehie, Suman Kumaria, Pramod Tandon
An efficient in vitro regeneration protocol has been developed from shoot tips of Drosera burmannii Vahl., a carnivorous plant of north-east India. Various plant growth regulators were used to study their efficacy in the induction of multiple shoots and roots. Of the various treatments, the maximum number of shoots (28.8 ± 1.5) and roots (9.7 ± 0.6) was observed in one-fourth strength standard medium (MS with 50 mg/l citric acid and 10 mg/l ascorbic acid) supplemented with 4 mg/l 6-benzylaminopurine (BAP) and 4 mg/l α-naphthalene acetic acid (NAA) followed by 26...
June 2017: 3 Biotech
https://www.readbyqxmd.com/read/28565774/effects-of-ankaferd-bloodstopper-on-bone-healing-in-an-ovariectomized-osteoporotic-rat-model
#16
Şeref Ezirganli, Hakki Oğuz Kazancioğlu, Ahmet Hüseyin Acar, Hakan Özdemir, Emre Kuzu, Deniz Şahin İnan
Bone defects resulting from oncological surgical resections, congenital facial anomalies, trauma or infection represent a significant and common clinical problem. The present study aimed to evaluate the effects of a commercially-available medicinal plant extract product, Ankaferd BloodStopper (ABS), on bone healing. The present study was performed on 24 female ovariectomized (OVX) rats. A defect on each rat calvarium was created using a trephine burr prior to the rats being divided into two groups. Defects were grafted with a gelatin sponge soaked with normal saline (control group) or plant extract (experimental group)...
May 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28563902/of-lemurs-and-louse-flies-the-biogeochemical-and-biotic-effects-of-forest-disturbance-on-propithecus-edwardsi-and-its-obligate-ectoparasite-allobosca-crassipes-in-ranomafana-national-park-southeastern-madagascar
#17
Elizabeth McGee, Stanley Vaughn
From alleles to ecosystems and landscapes, anthropogenic activity continues to affect the environment, with particularly adverse effects on biodiversity hotspots such as Madagascar. Selective logging has been proposed as a "win-win" conservation strategy, yet its effects on different components of biodiversity are still not fully understood. Here we examine biotic factors (i.e., dietary differences) that may be driving differences in biogeochemical stocks between disturbed and undisturbed forests. We present the stable nitrogen (δ(15) N) and carbon (δ(13) C) isotope composition of hair from the lemur Propithecus edwardsi and of whole bodies of its obligate ectoparasite, the louse-fly Allobosca crassipes, from sites in Ranomafana National Park (RNP) that are comparable except for the history of logging and subsequent forest regeneration...
May 31, 2017: American Journal of Primatology
https://www.readbyqxmd.com/read/28560765/an-amazonian-rainforest-and-its-fragments-as-a-laboratory-of-global-change
#18
William F Laurance, José L C Camargo, Philip M Fearnside, Thomas E Lovejoy, G Bruce Williamson, Rita C G Mesquita, Christoph F J Meyer, Paulo E D Bobrowiec, Susan G W Laurance
We synthesize findings from one of the world's largest and longest-running experimental investigations, the Biological Dynamics of Forest Fragments Project (BDFFP). Spanning an area of ∼1000 km(2) in central Amazonia, the BDFFP was initially designed to evaluate the effects of fragment area on rainforest biodiversity and ecological processes. However, over its 38-year history to date the project has far transcended its original mission, and now focuses more broadly on landscape dynamics, forest regeneration, regional- and global-change phenomena, and their potential interactions and implications for Amazonian forest conservation...
May 30, 2017: Biological Reviews of the Cambridge Philosophical Society
https://www.readbyqxmd.com/read/28558427/a-data-driven-approach-for-the-study-of-coagulation-phenomena-in-waste-lubricant-oils-and-its-relevance-in-alkaline-regeneration-treatments
#19
C T Pinheiro, V R Ascensão, M S Reis, M J Quina, L M Gando-Ferreira
Coagulation phenomena can occur in certain types of waste lubricant oils (WLO) during regeneration processes involving alkaline treatments, causing plant shutdowns. In this context, this study addresses the nature of the compounds responsible for the coagulation phenomena after the alkaline treatment. For such, an empirical test was developed to assess the coagulation behaviour of WLO, consisting in the addition of KOH to the WLO followed by heating under stirring conditions. This test was performed on 133 samples and four coagulation classes were identified: A; B1; B2 and C...
December 1, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28555622/zooming-in-on-the-phycosphere-the-ecological-interface-for-phytoplankton-bacteria-relationships
#20
REVIEW
Justin R Seymour, Shady A Amin, Jean-Baptiste Raina, Roman Stocker
By controlling nutrient cycling and biomass production at the base of the food web, interactions between phytoplankton and bacteria represent a fundamental ecological relationship in aquatic environments. Although typically studied over large spatiotemporal scales, emerging evidence indicates that this relationship is often governed by microscale interactions played out within the region immediately surrounding individual phytoplankton cells. This microenvironment, known as the phycosphere, is the planktonic analogue of the rhizosphere in plants...
May 30, 2017: Nature Microbiology
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