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https://www.readbyqxmd.com/read/28430402/a-removable-virus-vector-suitable-for-plant-genome-editing
#1
Tetsuya Chujo, Manabu Yoshikawa, Hirotaka Ariga, Masaki Endo, Seiichi Toki, Kazuhiro Ishibashi
Plant genome editing is achieved by expression of sequence-specific nucleases (SSNs). RNA virus vector-mediated expression of SSNs is a promising approach for transgene integration-free targeted mutagenesis in plants. However, removal of virus vectors from infected plants is challenging because no antiviral drugs against plant viruses are available. Here, we developed a removable RNA virus vector that carries the target site of tobacco microRNA398 (miR398) whose expression is induced during shoot regeneration...
April 21, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28429856/glycosyltransferase-cascades-for-natural-product-glycosylation-use-of-plant-instead-of-bacterial-sucrose-synthases-improves-the-udp-glucose-recycling-from-sucrose-and-udp
#2
Alexander Gutmann, Alexander Lepak, Margo Diricks, Tom Desmet, Bernd Nidetzky
Natural product glycosylations by Leloir glycosyltransferases (GTs) require expensive nucleotide-activated sugars as substrates. Sucrose synthase (SuSy) converts sucrose and uridine 5'-diphosphate (UDP) into UDP-glucose. Coupling of SuSy and GT reactions in one-pot cascade transformations creates a UDP cycle, which regenerates the UDP-glucose continuously and so makes it an expedient donor for glucoside production. Here we compare SuSys with divergent kinetic characteristics for UDP-glucose recycling in the synthesis of the natural C-glucoside nothofagin...
April 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28428810/recent-achievements-obtained-by-chloroplast-transformation
#3
REVIEW
Muhamed Adem, Dereje Beyene, Tileye Feyissa
Chloroplasts play a great role for sustained wellbeing of life on the planet. They have the power and raw materials that can be used as sophisticated biological factories. They are rich in energy as they have lots of pigment-protein complexes capable of collecting sunlight, in sugar produced by photosynthesis and in minerals imported from the plant cell. Chloroplast genome transformation offers multiple advantages over nuclear genome which among others, include: integration of the transgene via homologus recombination that enables to eliminate gene silencing and position effect, higher level of transgene expression resulting into higher accumulations of foreign proteins, and significant reduction in environmental dispersion of the transgene due to maternal inheritance which helps to minimize the major critic of plant genetic engineering...
2017: Plant Methods
https://www.readbyqxmd.com/read/28424873/the-changes-in-the-reproductive-barrier-between-hexaploid-wheat-triticum-aestivum-l-and-rye-secale-cereale-l-different-states-lead-to-different-fates
#4
Natalia Tikhenko, Twan Rutten, Angelika Senula, Myroslava Rubtsova, E R Joachim Keller, Andreas Börner
The changes in the reproductive barrier between hexaploid wheat ( Triticum aestivum L.) and rye ( Secale cereale L.) can be induced using in situ embryo rescue of abnormal embryos, yielding stable fertile amphidiploid plants. In intergeneric crosses between hexaploid wheat (Triticum aestivum L.) and rye (Secale cereale L.), postzygotic barriers may occur at different stages of hybrid development. One such mechanism is embryo lethality, which is genetically determined by the interaction and expression of two incompatible genes in wheat (Eml-A1) and rye (Eml-R1)...
April 19, 2017: Planta
https://www.readbyqxmd.com/read/28419724/a-reversed-photosynthesis-like-process-for-light-triggered-co2-capture-release-and-conversion
#5
Yapei Wang, Dingguan Wang, Shenglong Liao, Shiming Zhang
Materials for CO2 capture have been extensively exploited for climate governance and gaseous separation. However, their regeneration is facing the problems of high energy cost and secondary CO2 contamination. Herein, a reversed photosynthesis-like process is proposed, which absorbs CO2 in darkness while releases CO2 under light illumination. The process is likely supplementary to natural photosynthesis of plants which on the contrary release CO2 in nights. Remarkably, the material used in this work is able to capture 9...
April 16, 2017: ChemSusChem
https://www.readbyqxmd.com/read/28415512/study-of-epithelial-differentiation-and-protein-expression-of-keratinocyte-mesenchyme-stem-cell-co-cultivation-on-electrospun-nylon-b-vulgaris-extract-composite-scaffold
#6
Simzar Hosseinzadeh, Masoud Soleimani, Manuchehr Vossoughi, Parviz Ranjbarvan, Shokoh Hamedi, Soheila Zamanlui, Matin Mahmoudifard
Employing of the composite electrospun scaffold containing herbal extract in conjugation with co-culturing of cells can open up new window to the design of efficient biomaterials for skin tissue regeneration. Here, we introduce the synergistic effect of composite electrospun nanofibrous scaffold of nylon66 loaded with Beta vulgaris (B. vulgaris) (extract of beet roots, a plants whose widely used in Iranian folk medicine as wound healing medicine) and co-culture of mesenchymal stem-cells (MSCs)-human keratinocyte (H-keratino) differentiation towards epithelial lineage...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28414068/modulation-of-anthraquinones-and-phloroglucinols-biosynthesis-in-hypericum-spp-by-cryogenic-treatment
#7
Katarína Bruňáková, Eva Čellárová
Beside the high post-cryogenic recovery rate, a reinstated scale of secondary metabolites in recovered plant tissues represents another inevitable aspect of an effective cryopreservation protocol for medicinal plants. The current study was aimed at evaluation of the elicitation potential of cryogenic treatment on secondary metabolism of some Hypericum species. In agreement with our assumption, the cold stimuli applied during the pre-cryogenic phase increased the tolerance to low temperatures (-196°C) in H...
April 13, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28409921/mesenchymal-stem-cells-seeded-regenerated-silk-fibroin-complex-matrices-for-liver-regeneration-in-an-animal-model-of-acute-liver-failure
#8
Lijuan Xu, Shufang Wang, Xiang Sui, Yu Wang, Yinan Su, Li Huang, Yunwei Zhang, Zheng Chen, Qianqian Chen, Haitao Du, Yaopeng Zhang, Li Yan
The shortage of organ donation is the main limitation to liver transplantation as a treatment for the end-stage liver disease or acute liver failure. In order to develop an alternative therapy for acute liver failure, mesenchymal stem cells (MSCs) seeded regenerated silk fibroin (RSF) matrices were evaluated in vitro and in vivo. Adipose-derived mesenchymal stem cells (ADSCs) and bone marrow-derived mesenchymal stem cells (BMSCs) were planted and grown on RSF scaffolds to form scaffolds complex, respectively...
April 14, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28403551/cyclic-electron-flow-npq-and-photorespiration-are-crucial-for-the-establishment-of-young-plants-of-ricinus-communis-and-jatropha-curcas-exposed-to-drought
#9
Milton Costa Lima Neto, João Victor A Cerqueira, Juliana Ribeiro da Cunha, Rafael Vasconcelos Ribeiro, Joaquim Albenísio G Silveira
Although plant physiological responses to drought have been widely studied, the interaction between photoprotection, photorespiration and antioxidant metabolism in water-stressed plants is scarcely known. This study aimed to evaluate the physiological adjustments preserving photosynthesis and growth in two plant-species with differential tolerance to drought: Jatropha curcas and Ricinus communis. We measured stress indicators, gas exchange, photochemistry of PSII and PSI, antioxidant enzymes, cyclic electron flow and photorespiradion...
April 12, 2017: Plant Biology
https://www.readbyqxmd.com/read/28403182/functional-analysis-of-the-gmesr1-gene-associated-with-soybean-regeneration
#10
Chao Zhang, Xiaodong Wu, Binbin Zhang, Qingshan Chen, Ming Liu, Dawei Xin, Zhaoming Qi, Sinan Li, Yanlong Ma, Lingshuang Wang, Yangmei Jin, Wenbin Li, Xiaoxia Wu, An-Yu Su
Plant regeneration can occur via in vitro tissue culture through somatic embryogenesis or de novo shoot organogenesis. Transformation of soybean (Glycine max) is difficult, hence optimization of the transformation system for soybean regeneration is required. This study investigated ENHANCER OF SHOOT REGENERATION 1 (GmESR1), a soybean transcription factor that targets regeneration-associated genes. Sequence analysis showed that GmESR1 contained a conserved 57 amino acid APETALA 2 (AP2)/ETHYLENE RESPONSE FACTOR (ERF) DNA-binding domain...
2017: PloS One
https://www.readbyqxmd.com/read/28402593/a-new-method-for-the-production-of-sterile-colonies-of-lucilia-sericata
#11
N E Gasz, M L Harvey
Maggot debridement therapy (MDT) refers to the use of blowfly larvae to clean or debride an infected wound. Most commonly, larvae of Lucilia sericata (Meigen) (Diptera: Calliphoridae) are used, and are sterilized prior to use to ensure no further bacterial infections are introduced during treatment. Current methods sterilize eggs from laboratory-reared blowfly colonies, after which sterile early second instar maggots can be provided to hospitals for use in treatment. Maggots not required for treatment are used for colony regeneration, in which sterility is not maintained...
April 12, 2017: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/28401464/efficient-regeneration-and-improved-sonication-assisted-agrobacterium-transformation-saat-method-for-catharanthus-roseus
#12
Pravej Alam, Zainul Abdeen Khan, Malik Zainul Abdin, Jawaid A Khan, Parvaiz Ahmad, Shereen F Elkholy, Mahmoud A Sharaf-Eldin
Catharanthus roseus is an important medicinal plant known for its pharmacological qualities such as antimicrobial, anticancerous, antifeedant, antisterility, antidiabetic activities. More than 130 bioactive compounds like vinblastine, vindoline and vincristine have been synthesized in this plant. Extensive studies have been carried out for optimization regeneration and transformation protocols. Most of the protocol described are laborious and time-consuming. Due to sophisticated protocol of regeneration and genetic transformation, the production of these bioactive molecules is less and not feasible to be commercialized worldwide...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28401460/enhanced-production-of-berberine-in-in-vitro-regenerated-cell-of-tinospora-cordifolia-and-its-analysis-through-lcms-qtof
#13
Jitendra Mittal, Madan Mohan Sharma
Tinospora cordifolia is a prioritized medicinal plant and having an immense medicinal importance especially in Indian medicinal system. But this plant needs a regeneration protocol for its rapid propagation. An efficient regeneration protocol was developed for T. cordifolia using nodal explants. High frequency of multiple shoot formation was induced when the nodal segments were cultured on MS medium supplemented with BAP (1.0 mg L(-1)) and 2-iP (0.5 mg L(-1)). The highest mean number of shoots per nodal explant (7...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28398533/quantitative-proteomics-analysis-of-developmental-reprogramming-in-protoplast-of-the-moss-physcomitrella-patens
#14
Xiaoqin Wang, Lu Chen, Aizhen Yang, Chunya Bu, Yikun He
The moss Physcomitrella patens is a model system for studying developmental plant processes. To better understand the biochemical and physiological changes involved in developmental reprogramming, we conducted a quantitative proteomics analysis for protonemata, protoplasts made therefrom and protoplasts regenerated for two days. Using an iTRAQ peptide labelling strategy and liquid chromatography-tandem mass spectrometry (LC-MS/MS), more than 3000 peptides and 1000 proteins were quantified. Of these, 162 proteins were identified as having differential abundances during developmental reprogramming...
April 8, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28391480/plant-regeneration-from-cell-suspension-culture-in-saccharum-officinarum-l-and-ascertaining-of-genetic-fidelity-through-rapd-and-issr-markers
#15
Avinash S Thorat, Nishant A Sonone, Vrushali V Choudhari, Rachayya M Devarumath, K Harinath Babu
The aim of this study was to produce sugarcane plantlets from cell suspension culture and study its genetic fidelity using molecular markers. The study was carried out using sugarcane varieties Co 86032 and Q117. Callus cultures of both the varieties were optimized using six different callus induction media. After screening the growth response of callus on six different callus induction media, it was observed that medium no. VI supplemented with 500 mg l(-1) of each PVP, Casein hydrolysate and MES buffer showed high amounts of callus in Co 86032 (79...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28389923/dispersal-of-remnant-endangered-trees-in-a-fragmented-and-disturbed-forest-by-frugivorous-birds
#16
Ning Li, Bing Bai, Xin-Hai Li, Shu-Qing An, Chang-Hu Lu
Most endangered plant species in a fragmented forest behave as a unique source population, with a high dependence on frugivorous birds for recruitment and persistence. In this study, we combined field data of dispersal behavior of birds and GIS information of patch attributes to estimate how frugivorous birds could affect the effective dispersal pattern of Chinese yew (Taxus chinensis) in a fragmented and disturbed forest. Nine bird species were observed to visit T. chinensis trees, with Urocissa erythrorhyncha, Zoothera dauma and Picus canus being the most common dispersers...
April 7, 2017: Journal of Plant Research
https://www.readbyqxmd.com/read/28389585/a-two-step-model-for-de-novo-activation-of-wuschel-during-plant-shoot-regeneration
#17
Tian-Qi Zhang, Heng Lian, Chuan-Miao Zhou, Lin Xu, Yuling Jiao, Jia-Wei Wang
Plant cells are totipotent and competent to regenerate from differentiated organs. It has been known for six decades that cytokinin-rich medium induces shoot regeneration from callus cells. However, the underlying molecular mechanism remains elusive. The homeodomain transcription factor WUSCHEL (WUS) is essential for de novo establishment of the shoot stem cell niche in Arabidopsis thaliana. We found that WUS-positive cells (WUS+) mark the shoot progenitor region during regeneration. A cytokinin-rich environment initially promotes the removal of the repressive histone mark H3K27me3 at the WUS locus in a cell-cycle-dependent manner...
April 7, 2017: Plant Cell
https://www.readbyqxmd.com/read/28384317/decay-and-nutrient-dynamics-of-coarse-woody-debris-in-the-qinling-mountains-china
#18
Jie Yuan, Lin Hou, Xin Wei, Zhengchun Shang, Fei Cheng, Shuoxin Zhang
As an ecological unit, coarse woody debris (CWD) plays an essential role in productivity, nutrient cycling, carbon sequestration, community regeneration and biodiversity. However, thus far, the information on quantification the decomposition and nutrient content of CWD in forest ecosystems remains considerably limited. In this study, we conducted a long-term (1996-2013) study on decay and nutrient dynamics of CWD for evaluating accurately the ecological value of CWD on the Huoditang Experimental Forest Farm in the Qinling Mountains, China...
2017: PloS One
https://www.readbyqxmd.com/read/28376135/a-simple-and-efficient-protocol-for-the-cryopreservation-of-zygotic-embryos-of-macaw-palm-acrocomia-aculeata-jacq-lodd-ex-mart-a-tropical-species-with-a-capacity-for-biofuel-production
#19
Z G Luis, E Scherwinski-Pereira
BACKGROUND: The development of cryopreservation protocols for zygotic embryos has the advantage of direct embryo regeneration into plantlets, an important factor when one´s purpose is to preserve genetic plant resources. OBJECTIVE: Current study developed an efficient and simplified cryopreservation protocol for zygotic embryos of the macaw palm, a type of palm tree with great capacity for biodiesel production. MATERIALS AND METHODS: Embryos were obtained from seeds and rehydrated for 15 h in a 3% sucrose medium...
January 2017: Cryo Letters
https://www.readbyqxmd.com/read/28371610/short-exogenous-peptides-regulate-expression-of-cle-knox1-and-grf-family-genes-in-nicotiana-tabacum
#20
L I Fedoreyeva, T A Dilovarova, V V Ashapkin, Yu Ts Martirosyan, V Kh Khavinson, P N Kharchenko, B F Vanyushin
Exogenous short biologically active peptides epitalon (Ala-Glu-Asp-Gly), bronchogen (Ala-Glu-Asp-Leu), and vilon (Lys-Glu) at concentrations 10(-7)-10(-9) M significantly influence growth, development, and differentiation of tobacco (Nicotiana tabacum) callus cultures. Epitalon and bronchogen, in particular, both increase growth of calluses and stimulate formation and growth of leaves in plant regenerants. Because the regulatory activity of the short peptides appears at low peptide concentrations, their action to some extent is like that of the activity of phytohormones, and it seems to have signaling character and epigenetic nature...
April 2017: Biochemistry. Biokhimii︠a︡
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