keyword
https://read.qxmd.com/read/38479814/the-golgi-checkpoint-golgi-unlinking-during-g2-is-necessary-for-spindle-formation-and-cytokinesis
#1
JOURNAL ARTICLE
Fabiola Mascanzoni, Inmaculada Ayala, Roberta Iannitti, Alberto Luini, Antonino Colanzi
Entry into mitosis requires not only correct DNA replication but also extensive cell reorganization, including the separation of the Golgi ribbon into isolated stacks. To understand the significance of pre-mitotic Golgi reorganization, we devised a strategy to first block Golgi segregation, with the consequent G2-arrest, and then force entry into mitosis. We found that the cells forced to enter mitosis with an intact Golgi ribbon showed remarkable cell division defects, including spindle multipolarity and binucleation...
May 2024: Life Science Alliance
https://read.qxmd.com/read/38466841/the-arabidopsis-bub1-mad3-family-protein-bmf3-requires-bub3-3-to-recruit-cdc20-to-kinetochores-in-spindle-assembly-checkpoint-signaling
#2
JOURNAL ARTICLE
Xingguang Deng, Felicia Lei Peng, Xiaoya Tang, Yuh-Ru Julie Lee, Hong-Hui Lin, Bo Liu
The spindle assembly checkpoint (SAC) ensures faithful chromosome segregation during cell division by monitoring kinetochore-microtubule attachment. Plants produce both sequence-conserved and diverged SAC components, and it has been largely unknown how SAC activation leads to the assembly of these proteins at unattached kinetochores to prevent cells from entering anaphase. In Arabidopsis thaliana , the noncanonical BUB3.3 protein was detected at kinetochores throughout mitosis, unlike MAD1 and the plant-specific BUB1/MAD3 family protein BMF3 that associated with unattached chromosomes only...
March 19, 2024: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/38466053/comprehensive-analysis-indicated-that-nde1-is-a-potential-biomarker-for-pan-cancer-and-promotes-bladder-cancer-progression
#3
JOURNAL ARTICLE
Peihan Wang, Jinzhuo Ning, Wu Chen, Fan Zou, Weimin Yu, Ting Rao, Fan Cheng
BACKGROUND: The nuclear distribution E homologue 1 (NDE1) is a crucial dynein binding partner. The NDE1 protein has the potential to disrupt the normal functioning of centrosomes, leading to a compromised ability to generate spindles and ensure precise separation of chromosomes during cell division. The potential consequences of this phenomenon include genomic instability, malignant transformation and the proliferation of neoplastic growths. However, studies examining the connection between NDE1 and cancer is still very rare...
March 2024: Cancer Medicine
https://read.qxmd.com/read/38456964/kinesin-7-cenp-e-mediates-chromosome-alignment-and-spindle-assembly-checkpoint-in-meiosis-i
#4
JOURNAL ARTICLE
Jing-Lian Zhang, Meng-Fei Xu, Jie Chen, Ya-Lan Wei, Zhen-Yu She
In eukaryotes, meiosis is the genetic basis for sexual reproduction, which is important for chromosome stability and species evolution. The defects in meiosis usually lead to chromosome aneuploidy, reduced gamete number, and genetic diseases, but the pathogenic mechanisms are not well clarified. Kinesin-7 CENP-E is a key regulator in chromosome alignment and spindle assembly checkpoint in cell division. However, the functions and mechanisms of CENP-E in male meiosis remain largely unknown. In this study, we have revealed that the CENP-E gene was highly expressed in the rat testis...
March 8, 2024: Chromosoma
https://read.qxmd.com/read/38443684/retraction-note-the-long-noncoding-rna-crybg3-induces-aneuploidy-by-interfering-with-spindle-assembly-checkpoint-via-direct-binding-with-bub3
#5
Ziyang Guo, Yingchu Dai, Wentao Hu, Yongsheng Zhang, Zhifei Cao, Weiwei Pei, Ningang Liu, Jing Nie, Anqing Wu, Weidong Mao, Lei Chang, Bingyan Li, Hailong Pei, Tom K Hei, Guangming Zhou
No abstract text is available yet for this article.
March 5, 2024: Oncogene
https://read.qxmd.com/read/38410481/a-whole-genome-crispr-screen-identifies-the-spindle-accessory-checkpoint-as-a-locus-of-nab-paclitaxel-resistance-in-pancreatic-cancer-cells
#6
Priya Mondal, George Alyateem, Allison V Mitchell, Michael M Gottesman
Pancreatic adenocarcinoma is one of the most aggressive and lethal forms of cancer. Chemotherapy is the primary treatment for pancreatic cancer, but resistance to the drugs used remains a major challenge. A genome-wide CRISPR interference and knockout screen in the PANC-1 cell line with the drug nab-paclitaxel has identified a group of spindle assembly checkpoint (SAC) genes that enhance survival in nab-paclitaxel. Knockdown of these SAC genes (BUB1B, BUB3, and TTK) attenuates paclitaxel-induced cell death...
February 15, 2024: bioRxiv
https://read.qxmd.com/read/38402459/plk1-phosphorylation-of-zw10-guides-accurate-chromosome-segregation-in-mitosis
#7
JOURNAL ARTICLE
Sm Faysal Bellah, Fangyuan Xiong, Zhen Dou, Fengrui Yang, Xing Liu, Xuebiao Yao, Xinjiao Gao, Liangyu Zhang
Stable transmission of genetic information during cell division requires faithful chromosome segregation. Mounting evidence has demonstrated that PLK1 dynamics at kinetochores control correct kinetochore-microtubule attachments and subsequent silencing of the spindle checkpoint. However, the mechanisms underlying PLK1-mediated silencing of the spindle checkpoint remain elusive. Here, we identified a regulatory mechanism by which PLK1-elicited ZW10 phosphorylation regulates spindle checkpoint silencing in mitosis...
February 24, 2024: Journal of Molecular Cell Biology
https://read.qxmd.com/read/38396778/chromosome-division-in-early-embryos-is-everything-under-control-and-is-the-cell-size-important
#8
REVIEW
Adela Horakova, Marketa Konecna, Martin Anger
Chromosome segregation in female germ cells and early embryonic blastomeres is known to be highly prone to errors. The resulting aneuploidy is therefore the most frequent cause of termination of early development and embryo loss in mammals. And in specific cases, when the aneuploidy is actually compatible with embryonic and fetal development, it leads to severe developmental disorders. The main surveillance mechanism, which is essential for the fidelity of chromosome segregation, is the Spindle Assembly Checkpoint (SAC)...
February 9, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38393934/upregulation-of-vesicle-associated-membrane-protein-7-in-breast-cancer-tissues
#9
JOURNAL ARTICLE
Yu Huang, Mei Wu, Jian-Di Li, Zhen Qin, Ke-Qiang Huang, Jin-Zhu Cui, Hai-Ling Ou
BACKGROUND: Vesicle-associated membrane protein 7 (VAMP7) plays oncogenic roles in cancers. However, its clinical significance in breast cancer (BC) tissues remains unknown. OBJECTIVE: To elucidate the clinical implications of VAMP7, as well as its involvement in the tumor microenvironment and molecular pathways of breast cancer. METHODS: BC (n=100) and non-cancerous breast tissues (n= 100) were collected for an immunohistochemical experiment (1:200)...
January 30, 2024: Technology and Health Care: Official Journal of the European Society for Engineering and Medicine
https://read.qxmd.com/read/38384136/natural-killer-cell-based-signature-prognostic-analysis-in-head-and-neck-squamous-cell-carcinoma
#10
JOURNAL ARTICLE
Zizhao Guo, Yuxia Zhao, Meng Xu, Long Zhao, Xiaolei Wang
BACKGROUND: Head and neck squamous cell carcinoma (HNSC) is a challenging cancer with significant clinical implications. Natural killer (NK) cells have emerged as important players in tumor immunosurveillance, yet their role and potential as prognostic biomarkers in HNSC remain unclear. METHODS: Quantitative analysis using multiple algorithms identified FCRL1, KIR3DL2 and ZNF541 as molecules significantly associated with local NK cell infiltration and patient survival...
February 2024: Journal of Gene Medicine
https://read.qxmd.com/read/38375594/a-novel-hdac6-inhibitor-interferes-microtubule-dynamics-and-spindle-assembly-checkpoint-and-sensitizes-cisplatin-induced-apoptosis-in-castration-resistant-prostate-cancer
#11
JOURNAL ARTICLE
Pei-Chen Ye, Wohn-Jenn Leu, Tsung-Yu Yeh, Yu-Tung Hsu, Yi-Chin Lin, Zi-Yuan Wei, Yi-Chin Chen, Yi-Chang Chiang, Jui-Ling Hsu, She-Hung Chan, Lih-Ching Hsu, Ji-Wang Chern, Chao-Wu Yu, Jih-Hwa Guh
BACKGROUND: Metastatic castration-resistant prostate cancer (CRPC), the most refractory prostate cancer, inevitably progresses and becomes unresponsive to hormone therapy, revealing a pressing unmet need for this disease. Novel agents targeting HDAC6 and microtubule dynamics can be a potential anti-CRPC strategy. METHODS: Cell proliferation was examined in CRPC PC-3 and DU-145 cells using sulforhodamine B assay and anchorage-dependent colony formation assay. Flow cytometric analysis of propidium iodide staining was used to determine cell-cycle progression...
February 20, 2024: Prostate
https://read.qxmd.com/read/38372383/c-elegans-spermatocyte-divisions-show-weak-spindle-checkpoint-response
#12
JOURNAL ARTICLE
Shang-Yang Chen, Pu-Wei Cheng, Hsiao-Fang Peng, Jui-Ching Wu
Male meiotic division exhibits two consecutive chromosome separation events without apparent pausing. Several studies showed spermatocyte divisions are not stringently regulated as in mitotic cells. In this study, we investigated the role of the canonical spindle assembly (SAC) pathway in C. elegans spermatogenesis. We found the intensity of chromosome-associated outer kinetochore protein BUB-1 and SAC effector MDF-1 oscillate between two divisions. However, SAC target securin is degraded during the first division and remains undetectable for the second division...
February 19, 2024: Journal of Cell Science
https://read.qxmd.com/read/38370671/phospho-knl-1-recognition-by-a-tpr-domain-targets-the-bub-1-bub-3-complex-to-c-elegans-kinetochores
#13
Jack Houston, Clémence Vissotsky, Amar Deep, Hiro Hakozaki, Enice Crews, Karen Oegema, Kevin D Corbett, Pablo Lara-Gonzalez, Taekyung Kim, Arshad Desai
During mitosis, the Bub1-Bub3 complex concentrates at kinetochores, the microtubule-coupling interfaces on chromosomes, where it contributes to spindle checkpoint activation, kinetochore-spindle microtubule interactions, and protection of centromeric cohesion. Bub1 has a conserved N-terminal tetratricopeptide (TPR) domain followed by a binding motif for its conserved interactor Bub3. The current model for Bub1-Bub3 localization to kinetochores is that Bub3, along with its bound motif from Bub1, recognizes phosphorylated "MELT" motifs in the kinetochore scaffold protein Knl1...
February 11, 2024: bioRxiv
https://read.qxmd.com/read/38369783/pan-cancer-genomic-analysis-of-axl-mutations-reveals-a-novel-recurrent-functionally-activating-axl-w451c-alteration-specific-to-myxofibrosarcoma
#14
JOURNAL ARTICLE
Erik A Williams, Isabella Vegas, Fardous F El-Senduny, Jessica Zhang, Douglas A Mata, Matthew C Hiemenz, Sarah R Hughes, Brianna C Sa, Garrett P Kraft, Nicole Gorbatov, Kathleen Foley-Peres, Edward Z Sanchez, Clara Milikowski, Kevin Jon Williams, Jeffrey S Ross, Razelle Kurzrock, Elizabeth A Montgomery, David B Lombard, Surinder Kumar
Myxofibrosarcoma (MFS) is a common soft tissue sarcoma of the elderly that typically shows low tumor mutational burden, with mutations in TP53 and in genes associated with cell cycle checkpoints (RB1, CDKN2A). Unfortunately, no alterations or markers specific to MFS have been identified and, as a consequence, there are no effective targeted therapies. The receptor tyrosine kinase AXL, which drives cellular proliferation, is targetable by new antibody-based therapeutics. Expression of AXL messenger RNA is elevated in a variety of sarcoma types, with the highest levels reported in MFS, but the pathogenic significance of this finding remains unknown...
February 19, 2024: American Journal of Surgical Pathology
https://read.qxmd.com/read/38367607/chlorothalonil-exposure-compromised-mouse-oocyte-in-vitro-maturation-through-inducing-oxidative-stress-and-activating-mapk-pathway
#15
JOURNAL ARTICLE
Yong-Sheng Wang, Sheng-Ji Yang, Zi-Xuan Wan, Ao Shen, Muhammad Jamil Ahmad, Ming-Yue Chen, Li-Jun Huo, Jun-Hua Pan
Chlorothalonil (CTL) is widely used in agricultural production and antifoulant additive globally due to its broad spectrum and non-systemic properties, resulting in its widespread existence in foods, soil and water. Extensive evidence demonstrated that exposure to CTL induced adverse effects on organisms and in particular its reproductive toxicity has been attracted public concern. However, the influences of CTL on oocyte maturation is mysterious so far. In this study, we documented the toxic effects of CTL on oocyte in vitro maturation and the related underlying mechanisms...
February 16, 2024: Ecotoxicology and Environmental Safety
https://read.qxmd.com/read/38365687/the-dynamic-role-of-nucleoprotein-shcbp1-in-the-cancer-cell-cycle-and-its-potential-as-a-synergistic-target-for-dna-damaging-agents-in-cancer-therapy
#16
JOURNAL ARTICLE
Mei Zhou, Limin Duan, Jiangbin Chen, Yumei Li, Zhengrong Yin, Siwei Song, Yaqi Cao, Ping Luo, Fan Hu, Guanghai Yang, Juanjuan Xu, Tingting Liao, Yang Jin
BACKGROUND: Malignant tumours seriously threaten human life and health, and effective treatments for cancer are still being explored. The ability of SHC SH2 domain-binding protein 1 (SHCBP1) to induce cell cycle disturbance and inhibit tumour growth has been increasingly studied, but its dynamic role in the tumour cell cycle and corresponding effects leading to mitotic catastrophe and DNA damage have rarely been studied. RESULTS: In this paper, we found that the nucleoprotein SHCBP1 exhibits dynamic spatiotemporal expression during the tumour cell cycle, and SHCBP1 knockdown slowed cell cycle progression by inducing spindle disorder, as reflected by premature mitotic entry and multipolar spindle formation...
February 16, 2024: Cell Communication and Signaling: CCS
https://read.qxmd.com/read/38356703/the-significance-of-haus1-and-its-relationship-with-immune-microenvironment-in-hepatocellular-carcinoma
#17
JOURNAL ARTICLE
Lei Tang, Zhonghuo Chen, Chao Wei, Hao Liu, Ben Wang, Taozhi Yu, Xiaofei Tao, Jiale Yang, Jiafu Guan, Jianwei Yi, Hengchang Zhu, Chen Li, Peng Tang, Kai Wang
Background: HAUS Augmin-like complex subunit 1(HAUS1), as a controlling gene, which affected the production of spindle was firstly discovered in Drosophila cells. Although HAUS1 has been intensively studied, but its significance and relationship with the immune microenvironment in Hepatocellular carcinoma (HCC) remain unclear. Materials and Methods: All data of HCC in this paper were obtained from The Cancer Genome Atlas(TCGA), Genotype-Tissue Expression (GTEx), Gene Expression Omnibus (GEO) and the Human Protein Atlas(HPA) database...
2024: Journal of Cancer
https://read.qxmd.com/read/38354735/a-conserved-cenp-e-region-mediates-bubr1-independent-recruitment-to-the-outer-corona-at-mitotic-onset
#18
JOURNAL ARTICLE
Jeraldine Weber, Thibault Legal, Alicia Perez Lezcano, Agata Gluszek-Kustusz, Calum Paterson, Susana Eibes, Marin Barisic, Owen R Davies, Julie P I Welburn
The outer corona plays an essential role at the onset of mitosis by expanding to maximize microtubule attachment to kinetochores.1 , 2 The low-density structure of the corona forms through the expansion of unattached kinetochores. It comprises the RZZ complex, the dynein adaptor Spindly, the plus-end directed microtubule motor centromere protein E (CENP-E), and the Mad1/Mad2 spindle-assembly checkpoint proteins.3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 CENP-E specifically associates with unattached kinetochores to facilitate chromosome congression,11 , 12 , 13 , 14 , 15 , 16 interacting with BubR1 at the kinetochore through its C-terminal region (2091-2358)...
February 8, 2024: Current Biology: CB
https://read.qxmd.com/read/38337031/functional-analysis-of-cdc20-reveals-a-critical-role-of-cry-box-in-mitotic-checkpoint-signaling
#19
JOURNAL ARTICLE
Yuqing Zhang, Rose Young, Dimitriya H Garvanska, Chunlin Song, Yujing Zhai, Ying Wang, Hongfei Jiang, Jing Fang, Jakob Nilsson, Claudio Alfieri, Gang Zhang
Accurate mitosis is coordinated by the spindle assembly checkpoint (SAC) through the mitotic checkpoint complex (MCC), which inhibits the anaphase-promoting complex or cyclosome (APC/C). As an essential regulator, Cdc20 promotes mitotic exit through activating APC/C and monitors kinetochore-microtubule attachment through activating SAC. Cdc20 requires multiple interactions with APC/C and MCC subunits to elicit these functions. Functionally assessing these interactions within cells requires efficient depletion of endogenous Cdc20, which is highly difficult to achieve by RNA interference (RNAi)...
February 9, 2024: Communications Biology
https://read.qxmd.com/read/38324016/co-regulation-of-ndc80-complex-subunits-determines-the-fidelity-of-the-spindle-assembly-checkpoint-and-mitosis
#20
JOURNAL ARTICLE
Se Hong Kim, Thomas T Y Lau, Man Kit Liao, Hoi Tang Ma, Randy Y C Poon
NDC80 complex (NDC80C) is composed of four subunits (SPC24, SPC25, NDC80, and NUF2) and is vital for kinetochore-microtubule (KT-MT) attachment during mitosis. Paradoxically, NDC80C also functions in the activation of the spindle-assembly checkpoint (SAC). This raises an interesting question regarding how mitosis is regulated when NDC80C levels are compromised. Using a degron-mediated depletion system, we found that acute silencing of SPC24 triggered a transient mitotic arrest followed by mitotic slippage. SPC24-deficient cells were unable to sustain SAC activation despite the loss of KT-MT interaction...
February 7, 2024: Molecular Cancer Research: MCR
keyword
keyword
49963
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.